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Sequences

Sequence MSLN.cAug05

CCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGGTGTGGCCAATCACCCTGCACATCAGAGTTACCCTGGGCAGGGCC CACTGAGACCTGGGAGGGGCCACTCGGGACCTGGAGGGCTGGGGGCTGCCCGGGCGTTAGGGGTAAAGCTCCCTACCCAACTGCGCAGAAGGCCTCAGAG GCCTGGGGGCTGGGCTTCCCCTTTCACATCGCCCTTTAGAGGCCCACGTGTGGGCATTGGCCCGCGATCTGAAAGGGGCTGTCCTGTTCCTCATGGGCGC TGCCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCCCGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCC TGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCT CGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTC TCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCA AGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCC AGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGG CTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGC GCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTGGCGGCAGCCTGA ACGGACCATCCTCCGGCCGCGGTTCCGGCGGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTAC AAGAAGTGGGAGCTGGAAGCCTGCGTGGATGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATCCCCTTCACCTACGAGCAGCTGGACGTCC TAAAGCATAAACTGGATGAGCTCTACCCACAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGCCCTGAGGACATTCG CAAGTGGAATGTGACGTCCCTGGAGACCCTGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTT GTGAAGGGAAGGGGCCAGCTAGACAAAGACACCCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCG TGCCCCCCAGCAGCATCTGGGCGGTCAGGCCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCCCGCCTTGCTTTCCA GAACATGAACGGGTCCGAATACTTCGTGAAGATCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATG GACTTGGCCACGTTCATGAAGCTGCGGACGGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTTCTGGGACCCCACGTGGAGGGCCTGAAGG CGGAGGAGCGGCACCGCCCGGTGCGGGACTGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGCGGCATCCCCAACGG CTACCTGGTCCTAGACCTCAGCATGCAAGGTGGGCGGGGCGGCCAGGCCAGGGCTGGGGGCAGAGCTGGGGGCGTGGAGGTGGGCGCTCTGAGTCACCCC TCTCTCTGTAGAGGCCCTCTCGGGGACGCCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACCCTGGCCTGAGGG CCCCACTCCCTTGCTGGCCCCAGCCCTGCTGGGGATCCCCGCCTGGCCAGGAGCAGGCACGGGTGGTCCCCGTTCCACCCCAAGAGAACTCGCGCTCAGT AAACGGGAACATGCCCCCTGCAGACACGT

MSLN.F11 (forward primer)
MSLN.R5 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

PGSPPPLPN*GNRGRGGSGVANHPAHQSYPGQG PLRPGRGHSGPGGLGAARALGVKLPTQLRRRPQR PGGWASPFTSPFRGPRVGIGPRSERGCPVPHGR CQRHALLFLPGRPLPSAVTRGQRATGGPTVPPS LGSTQTMALPTARPLLGSCGTPALGSLLFLLFSL GWVQPSRTLAGETGQEAAPLDGVLANPPNISSL SPRQLLGFPCAEVSGLSTERVRELAVALAQKNV KLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNP DAFSGPQACTRFFSRITKANVDLLPRGAPERQR LLPAALACWGVRGSLLSEADVRALGGLACDLPG RFVAESAEVLLPRLGIVAAWRQRSSRDPSWRQPE RTILRPRFRREVEKTACPSGKKAREIDESLIFY KKWELEACVDAALLATQMDRVNAIPFTYEQLDV LKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIR KWNVTSLETLKALLEVNKGHEMSPQVATLIDRF VKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSS VPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQ NMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSM DLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLK AEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNG YLVLDLSMQGGRGGQARAGGRAGGVEVGALSHP SLCRGPLGDALPPRTWTCSHRPGTAPSLHPGLR APLPCWPQPCWGSPPGQEQARVVPVPPQENSRSV NGNMPPADT

Frame 1

QALHPHFPIEETEAEEAQVWPITLHIRVTLGRA H*DLGGATRDLEGWGLPGR*G*SSLPNCAEGLR GLGAGLPLSHRPLEAHVWALARDLKGAVLFLMGA ASATHSSFCLAGHSRLL*RADRELPVDPRCLPP WDLHRPWPCQRLDPCWGPVGPPPSAASCSCSSA SDGCSPRGPWLERQGRRLRPWTESWPTHLTFPAS PLANSLASRVRRCPA*ARSVSGSWLWPWHRRMS SSQQSSCAVWLTGSLSPPRTWTPSHWTCCYSST QMRSRGPRPAPVSSPASRRPMWTCSRGGLPSDSG CCLRLWPAGVCGGLC*ARLMCGLWEAWLATCLG ALWPSRPKCCYPGWASWPRGGNAPLGTHPGGSL NGPSSGRGSGGKWRRQPVLQARRPAR*TRASSST RSGSWKPAWMRPCWPPRWTA*TPSPSPTSSWTS *SINWMSSTHKVTPSL*SSTWATSSSR*ALRTF ASGM*RPWRP*RLCLKSTKGTK*VLRWPP*STAL *REGAS*TKTP*TP*PPSTLGTCAPSAPRS*AP CPPAASGRSGPRTWTRVTQGSWTSSIPRPALLS RT*TGPNTS*RSSPSWVGPPRRI*RRSVSRM*AW TWPRS*SCGRMRCCR*LWLRCRNFWDPTWRA*R RRSGTARCGTGSYGSGRTTWTRWGWGYRAASPT ATWS*TSACKVGGAARPGLGAELGAWRWAL*VTP LSVEALSGTPCLLGPGPVLTVLALLLASTLA*G PHSLAGPSPAGDPRLARSRHGWSPFHPKRTRAQ *TGTCPLQTR

Frame 2

RLSTPTSQLRKPRQRRLRCGQSPCTSELPWAGP TETWEGPLGTWRAGGCPGVRGKAPYPTAQKASE AWGLGFPFHIAL*RPTCGHWPAI*KGLSCSSWA LPAPRTPLSAWPATPVCCDARTESYRWTHGASLP GIYTDHGLANGSTPVGVLWDPRPRQPPVPALQP RMGAALEDPGWRDRAGGCAPGRSPGQPT*HFQP LPSPTPWLPVCGGVRPEHGACPGAGCGLGTEECQ ALNRAAALSGSPAL*APRGPGRPPIGPAAIPQP RCVLGAPGLHPFLLPHHEGQCGPAPEGGSRATA AAACGSGLLGCAGVSAERG*CAGSGRPGLRPAWA LCGRVGRSAATPAGHRGRVAATLLSGPILAAA* TDHPPAAVPAGSGEDSLSFRQEGPRDRREPHLL QEVGAGSLRGCGPAGHPDGPRERHPLHLRAAGRP KA*TG*ALPTRLPRVCDPAPGLPLPQDEP*GHS QVECDVPGDPEGFA*SQQRARNESSGGHPDRPL CEGKGPARQRHPRHPDRLLPWVPVLPQPRGAELR APQQHLGGQAPGPGHV*PKAAGRPLSQGPPCFP EHERVRILREDPVLPGWGPHGGFEGAQSAECEH GLGHVHEAADGCGAAVDCG*GAETSGTPRGGPEG GGAAPPGAGLDPTAAAGRPGHAGAGATGRHPQR LPGPRPQHARWAGRPGQGWGQSWGRGGGRSESP LSL*RPSRGRPAS*DLDLFSPSWHCS*PPPWPEG PTPLLAPALLGIPAWPGAGTGGPRSTPRELALS KREHAPCRH

Sequence MSLN.fAug05

CCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGGTGTGGCCAATCACCCTGCACATCAGAGTTACCCTGGGCAGGGCC CACTGAGACCTGGGAGGGGCCACTCGGGACCTGGAGGGCTGGGGGCTGCCCGGGCGTTAGGGGTAAAGCTCCCTACCCAACTGCGCAGAAGGCCTCAGAG GCCTGGGGGCTGGGCTTCCCCTTTCACATCGCCCTTTAGAGGCCCACGTGTGGGCATTGGCCCGCGATCTGAAAGGGGCTGTCCTGTTCCTCATGGGCGC TGCCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCCCGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCC TGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCT CGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTC TCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCA AGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCC AGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGG CTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGC GCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAGGAGGCAGCCAGGGCGGCTCTGCA GGGCGGGGGACCCCCCTACGGCCCCCCGTCGACATGGTCTGTCTCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATCCGC AGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGC GGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTACAAGAAGTGGGAGCTGGAAGCCTGCGTGGA TGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATCCCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTACCCA CAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGCCCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCC TGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTTGTGAAGGGAAGGGGCCAGCTAGACAAAGA CACCCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCCCCCAGCAGCATCTGGGCGGTCAGG CCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCCCGCCTTGCTTTCCAGAACATGAACGGGTCCGAATACTTCGTGA AGATCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATGGACTTGGCCACGTTCATGAAGCTGCGGAC GGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTTCTGGGACCCCACGTGGAGGGCCTGAAGGCGGAGGAGCGGCACCGCCCGGTGCGGGAC TGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGCGGCATCCCCAACGGCTACCTGGTCCTAGACCTCAGCATGCAAG GACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACCCTGGCCTGAGGGCCCCACTCCCTTGCTGGCCCCAGCCCTGCTGGGGATCCCCG CCTGGCCAGGAGCAGGCACGGGTGGTCCCCGTTCCACCCCAAGAGAACTCGCGCTCAGTAAACGGGAACATGCCCCCTGCAGACACGT

MSLN.F11 (forward primer)
MSLN.R5 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

PGSPPPLPN*GNRGRGGSGVANHPAHQSYPGQG PLRPGRGHSGPGGLGAARALGVKLPTQLRRRPQR PGGWASPFTSPFRGPRVGIGPRSERGCPVPHGR CQRHALLFLPGRPLPSAVTRGQRATGGPTVPPS LGSTQTMALPTARPLLGSCGTPALGSLLFLLFSL GWVQPSRTLAGETGQEAAPLDGVLANPPNISSL SPRQLLGFPCAEVSGLSTERVRELAVALAQKNV KLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNP DAFSGPQACTRFFSRITKANVDLLPRGAPERQR LLPAALACWGVRGSLLSEADVRALGGLACDLPG RFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQ GGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIR SIPQGIVAAWRQRSSRDPSWRQPERTILRPRFR REVEKTACPSGKKAREIDESLIFYKKWELEACVD AALLATQMDRVNAIPFTYEQLDVLKHKLDELYP QGYPESVIQHLGYLFLKMSPEDIRKWNVTSLET LKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKD TLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVR PQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFV KIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRT DAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRD WILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQ GPGPVLTVLALLLASTLA*GPHSLAGPSPAGDPR LARSRHGWSPFHPKRTRAQ*TGTCPLQTR

Frame 1

QALHPHFPIEETEAEEAQVWPITLHIRVTLGRA H*DLGGATRDLEGWGLPGR*G*SSLPNCAEGLR GLGAGLPLSHRPLEAHVWALARDLKGAVLFLMGA ASATHSSFCLAGHSRLL*RADRELPVDPRCLPP WDLHRPWPCQRLDPCWGPVGPPPSAASCSCSSA SDGCSPRGPWLERQGRRLRPWTESWPTHLTFPAS PLANSLASRVRRCPA*ARSVSGSWLWPWHRRMS SSQQSSCAVWLTGSLSPPRTWTPSHWTCCYSST QMRSRGPRPAPVSSPASRRPMWTCSRGGLPSDSG CCLRLWPAGVCGGLC*ARLMCGLWEAWLATCLG ALWPSRPKCCYPGW*AARDPWTRTSRRQPGRLC RAGDPPTAPRRHGLSPRWTLCGACCPCWASPSSA ASRRASWPRGGNAPLGTHPGGSLNGPSSGRGSG GKWRRQPVLQARRPAR*TRASSSTRSGSWKPAW MRPCWPPRWTA*TPSPSPTSSWTS*SINWMSSTH KVTPSL*SSTWATSSSR*ALRTFASGM*RPWRP *RLCLKSTKGTK*VLRWPP*STAL*REGAS*TK TP*TP*PPSTLGTCAPSAPRS*APCPPAASGRSG PRTWTRVTQGSWTSSIPRPALLSRT*TGPNTS* RSSPSWVGPPRRI*RRSVSRM*AWTWPRS*SCG RMRCCR*LWLRCRNFWDPTWRA*RRRSGTARCGT GSYGSGRTTWTRWGWGYRAASPTATWS*TSACK DLDLFSPSWHCS*PPPWPEGPTPLLAPALLGIP AWPGAGTGGPRSTPRELALSKREHAPCRH

Frame 2

RLSTPTSQLRKPRQRRLRCGQSPCTSELPWAGP TETWEGPLGTWRAGGCPGVRGKAPYPTAQKASE AWGLGFPFHIAL*RPTCGHWPAI*KGLSCSSWA LPAPRTPLSAWPATPVCCDARTESYRWTHGASLP GIYTDHGLANGSTPVGVLWDPRPRQPPVPALQP RMGAALEDPGWRDRAGGCAPGRSPGQPT*HFQP LPSPTPWLPVCGGVRPEHGACPGAGCGLGTEECQ ALNRAAALSGSPAL*APRGPGRPPIGPAAIPQP RCVLGAPGLHPFLLPHHEGQCGPAPEGGSRATA AAACGSGLLGCAGVSAERG*CAGSGRPGLRPAWA LCGRVGRSAATPAGELPGTPGPGPAGGSQGGSA GRGTPLRPPVDMVCLHDGRSAGPAARAGPAHHP QHPAGHRGRVAATLLSGPILAAA*TDHPPAAVPA GSGEDSLSFRQEGPRDRREPHLLQEVGAGSLRG CGPAGHPDGPRERHPLHLRAAGRPKA*TG*ALP TRLPRVCDPAPGLPLPQDEP*GHSQVECDVPGDP EGFA*SQQRARNESSGGHPDRPLCEGKGPARQR HPRHPDRLLPWVPVLPQPRGAELRAPQQHLGGQ APGPGHV*PKAAGRPLSQGPPCFPEHERVRILRE DPVLPGWGPHGGFEGAQSAECEHGLGHVHEAAD GCGAAVDCG*GAETSGTPRGGPEGGGAAPPGAG LDPTAAAGRPGHAGAGATGRHPQRLPGPRPQHAR TWTCSHRPGTAPSLHPGLRAPLPCWPQPCWGSP PGQEQARVVPVPPQENSRSVNGNMPPADT

Sequence MSLN.eAug05

CCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGGTGTGGCCAATCACCCTGCACATCAGAGTTACCCTGGGCAGGGCC CACTGAGACCTGGGAGGGGCCACTCGGGACCTGGAGGGCTGGGGGCTGCCCGGGCGTTAGGGGTAAAGCTCCCTACCCAACTGCGCAGAAGGCCTCAGAG GCCTGGGGGCTGGGCTTCCCCTTTCACATCGCCCTTTAGAGGCCCACGTGTGGGCATTGGCCCGCGATCTGAAAGGGGCTGTCCTGTTCCTCATGGGCGC TGCCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCCCGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCC TGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCT CGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTC TCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCA AGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCC AGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGG CTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGC GCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAGGAGGCAGCCAGGGCGGCTCTGCA GGGCGGGGGACCCCCCTACGGCCCCCCGTCGACATGGTCTGTCTCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATCCGC AGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGC GGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTACAAGAAGTGGGAGCTGGAAGCCTGCGTGGA TGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATCCCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTACCCA CAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGCCCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCC TGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCCTCAGGCTCCTCGGCGGCCCCTCCCACAGGTGGCCACCCTGATCGACCGCTTTGTGAA GGGAAGGGGCCAGCTAGACAAAGACACCCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCC CCCAGCAGCATCTGGGCGGTCAGGCCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCCCGCCTTGCTTTCCAGAACA TGAACGGGTCCGAATACTTCGTGAAGATCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATGGACTT GGCCACGTTCATGAAGCTGCGGACGGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTTCTGGGACCCCACGTGGAGGGCCTGAAGGCGGAG GAGCGGCACCGCCCGGTGCGGGACTGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGCGGCATCCCCAACGGCTACC TGGTCCTAGACCTCAGCATGCAAGAGGCCCTCTCGGGGACGCCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCAC CCTGGCCTGAGGGCCCCACTCCCTTGCTGGCCCCAGCCCTGCTGGGGATCCCCGCCTGGCCAGGAGCAGGCACGGGTGGTCCCCGTTCCACCCCAAGAGA ACTCGCGCTCAGTAAACGGGAACATGCCCCCTGCAGACACGT

MSLN.F11 (forward primer)
MSLN.R5 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

PGSPPPLPN*GNRGRGGSGVANHPAHQSYPGQG PLRPGRGHSGPGGLGAARALGVKLPTQLRRRPQR PGGWASPFTSPFRGPRVGIGPRSERGCPVPHGR CQRHALLFLPGRPLPSAVTRGQRATGGPTVPPS LGSTQTMALPTARPLLGSCGTPALGSLLFLLFSL GWVQPSRTLAGETGQEAAPLDGVLANPPNISSL SPRQLLGFPCAEVSGLSTERVRELAVALAQKNV KLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNP DAFSGPQACTRFFSRITKANVDLLPRGAPERQR LLPAALACWGVRGSLLSEADVRALGGLACDLPG RFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQ GGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIR SIPQGIVAAWRQRSSRDPSWRQPERTILRPRFR REVEKTACPSGKKAREIDESLIFYKKWELEACVD AALLATQMDRVNAIPFTYEQLDVLKHKLDELYP QGYPESVIQHLGYLFLKMSPEDIRKWNVTSLET LKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVK GRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVP PSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQN MNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDL ATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAE ERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGY LVLDLSMQEALSGTPCLLGPGPVLTVLALLLAST LA*GPHSLAGPSPAGDPRLARSRHGWSPFHPKR TRAQ*TGTCPLQTR

Frame 1

QALHPHFPIEETEAEEAQVWPITLHIRVTLGRA H*DLGGATRDLEGWGLPGR*G*SSLPNCAEGLR GLGAGLPLSHRPLEAHVWALARDLKGAVLFLMGA ASATHSSFCLAGHSRLL*RADRELPVDPRCLPP WDLHRPWPCQRLDPCWGPVGPPPSAASCSCSSA SDGCSPRGPWLERQGRRLRPWTESWPTHLTFPAS PLANSLASRVRRCPA*ARSVSGSWLWPWHRRMS SSQQSSCAVWLTGSLSPPRTWTPSHWTCCYSST QMRSRGPRPAPVSSPASRRPMWTCSRGGLPSDSG CCLRLWPAGVCGGLC*ARLMCGLWEAWLATCLG ALWPSRPKCCYPGW*AARDPWTRTSRRQPGRLC RAGDPPTAPRRHGLSPRWTLCGACCPCWASPSSA ASRRASWPRGGNAPLGTHPGGSLNGPSSGRGSG GKWRRQPVLQARRPAR*TRASSSTRSGSWKPAW MRPCWPPRWTA*TPSPSPTSSWTS*SINWMSSTH KVTPSL*SSTWATSSSR*ALRTFASGM*RPWRP *RLCLKSTKGTK*VLRLLGGPSHRWPP*STAL* REGAS*TKTP*TP*PPSTLGTCAPSAPRS*APCP PAASGRSGPRTWTRVTQGSWTSSIPRPALLSRT *TGPNTS*RSSPSWVGPPRRI*RRSVSRM*AWT WPRS*SCGRMRCCR*LWLRCRNFWDPTWRA*RRR SGTARCGTGSYGSGRTTWTRWGWGYRAASPTAT WS*TSACKRPSRGRPAS*DLDLFSPSWHCS*PP PWPEGPTPLLAPALLGIPAWPGAGTGGPRSTPRE LALSKREHAPCRH

Frame 2

RLSTPTSQLRKPRQRRLRCGQSPCTSELPWAGP TETWEGPLGTWRAGGCPGVRGKAPYPTAQKASE AWGLGFPFHIAL*RPTCGHWPAI*KGLSCSSWA LPAPRTPLSAWPATPVCCDARTESYRWTHGASLP GIYTDHGLANGSTPVGVLWDPRPRQPPVPALQP RMGAALEDPGWRDRAGGCAPGRSPGQPT*HFQP LPSPTPWLPVCGGVRPEHGACPGAGCGLGTEECQ ALNRAAALSGSPAL*APRGPGRPPIGPAAIPQP RCVLGAPGLHPFLLPHHEGQCGPAPEGGSRATA AAACGSGLLGCAGVSAERG*CAGSGRPGLRPAWA LCGRVGRSAATPAGELPGTPGPGPAGGSQGGSA GRGTPLRPPVDMVCLHDGRSAGPAARAGPAHHP QHPAGHRGRVAATLLSGPILAAA*TDHPPAAVPA GSGEDSLSFRQEGPRDRREPHLLQEVGAGSLRG CGPAGHPDGPRERHPLHLRAAGRPKA*TG*ALP TRLPRVCDPAPGLPLPQDEP*GHSQVECDVPGDP EGFA*SQQRARNESSGSSAAPPTGGHPDRPLCE GKGPARQRHPRHPDRLLPWVPVLPQPRGAELRA PQQHLGGQAPGPGHV*PKAAGRPLSQGPPCFPEH ERVRILREDPVLPGWGPHGGFEGAQSAECEHGL GHVHEAADGCGAAVDCG*GAETSGTPRGGPEGG GAAPPGAGLDPTAAAGRPGHAGAGATGRHPQRLP GPRPQHARGPLGDALPPRTWTCSHRPGTAPSLH PGLRAPLPCWPQPCWGSPPGQEQARVVPVPPQE NSRSVNGNMPPADT

Sequence MSLN.dAug05

GGAGGCTGCCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCCTGG GATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCTCGG ATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTCTCC CCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCAAGC TCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCCAGA TGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGGCTG CTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGCGCT TTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAGGAGGCAGCCAGGGCGGCTCTGCAGGG CGGGGGACCCCCCTACGGCCCCCCGTCGACATGGTCTGTCTCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATCCGCAGC ATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGCGGG AAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTACAAGAAGTGGGAGCTGGAAGCCTGCGTGGATGC GGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATCCCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTACCCACAA GGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGCCCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCCTGA AGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTTGTGAAGGGAAGGGGCCAGCTAGACAAAGACAC CCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCCCCCAGCAGCATCTGGGCGGTCAGGCCC CAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCCCGCCTTGCTTTCCAGAACATGAACGGGTCCGAATACTTCGTGAAGA TCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATGGACTTGGCCACGTTCATGAAGCTGCGGACGGA TGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTTCTGGGACCCCACGTGGAGGGCCTGAAGGCGGAGGAGCGGCACCGCCCGGTGCGGGACTGG ATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGCGGCATCCCCAACGGCTACCTGGTCCTAGACCTCAGCATGCAAGAGG CCCTCTCGGGGACGCCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACCCTGGCCTGAGGGCCCCACTCCCTTGC TGGCCCCAGCCCTGCTGGGGATCCCCGCCTGGCCAGGAGCAGGCACGGGTGGTCCCCGTTCCACCCCAAGAGAACTCGCGCTCAGTAAACGGGAACATGC CCCCTGCAGACACGT

MSLN.F11 (forward primer)
MSLN.R5 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

GGCQALHPHFPIEETEAEEAQRATGGPTVPPSL GSTQTMALPTARPLLGSCGTPALGSLLFLLFSLG WVQPSRTLAGETGQEAAPLDGVLANPPNISSLS PRQLLGFPCAEVSGLSTERVRELAVALAQKNVK LSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPD AFSGPQACTRFFSRITKANVDLLPRGAPERQRL LPAALACWGVRGSLLSEADVRALGGLACDLPGR FVAESAEVLLPRLVSCPGPLDQDQQEAARAALQG GGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRS IPQGIVAAWRQRSSRDPSWRQPERTILRPRFRR EVEKTACPSGKKAREIDESLIFYKKWELEACVDA ALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQ GYPESVIQHLGYLFLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDT LDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRP QDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVK IQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTD AVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDW ILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQE ALSGTPCLLGPGPVLTVLALLLASTLA*GPHSLA GPSPAGDPRLARSRHGWSPFHPKRTRAQ*TGTC PLQTR

Frame 1

EAARLSTPTSQLRKPRQRRLRELPVDPRCLPPW DLHRPWPCQRLDPCWGPVGPPPSAASCSCSSAS DGCSPRGPWLERQGRRLRPWTESWPTHLTFPASP LANSLASRVRRCPA*ARSVSGSWLWPWHRRMSS SQQSSCAVWLTGSLSPPRTWTPSHWTCCYSSTQ MRSRGPRPAPVSSPASRRPMWTCSRGGLPSDSGC CLRLWPAGVCGGLC*ARLMCGLWEAWLATCLGA LWPSRPKCCYPGW*AARDPWTRTSRRQPGRLCR AGDPPTAPRRHGLSPRWTLCGACCPCWASPSSAA SRRASWPRGGNAPLGTHPGGSLNGPSSGRGSGG KWRRQPVLQARRPAR*TRASSSTRSGSWKPAWM RPCWPPRWTA*TPSPSPTSSWTS*SINWMSSTHK VTPSL*SSTWATSSSR*ALRTFASGM*RPWRP* RLCLKSTKGTK*VLRWPP*STAL*REGAS*TKT P*TP*PPSTLGTCAPSAPRS*APCPPAASGRSGP RTWTRVTQGSWTSSIPRPALLSRT*TGPNTS*R SSPSWVGPPRRI*RRSVSRM*AWTWPRS*SCGR MRCCR*LWLRCRNFWDPTWRA*RRRSGTARCGTG SYGSGRTTWTRWGWGYRAASPTATWS*TSACKR PSRGRPAS*DLDLFSPSWHCS*PPPWPEGPTPL LAPALLGIPAWPGAGTGGPRSTPRELALSKREHA PCRH

Frame 2

RLPGSPPPLPN*GNRGRGGSESYRWTHGASLPG IYTDHGLANGSTPVGVLWDPRPRQPPVPALQPR MGAALEDPGWRDRAGGCAPGRSPGQPT*HFQPL PSPTPWLPVCGGVRPEHGACPGAGCGLGTEECQA LNRAAALSGSPAL*APRGPGRPPIGPAAIPQPR CVLGAPGLHPFLLPHHEGQCGPAPEGGSRATAA AACGSGLLGCAGVSAERG*CAGSGRPGLRPAWAL CGRVGRSAATPAGELPGTPGPGPAGGSQGGSAG RGTPLRPPVDMVCLHDGRSAGPAARAGPAHHPQ HPAGHRGRVAATLLSGPILAAA*TDHPPAAVPAG SGEDSLSFRQEGPRDRREPHLLQEVGAGSLRGC GPAGHPDGPRERHPLHLRAAGRPKA*TG*ALPT RLPRVCDPAPGLPLPQDEP*GHSQVECDVPGDPE GFA*SQQRARNESSGGHPDRPLCEGKGPARQRH PRHPDRLLPWVPVLPQPRGAELRAPQQHLGGQA PGPGHV*PKAAGRPLSQGPPCFPEHERVRILRED PVLPGWGPHGGFEGAQSAECEHGLGHVHEAADG CGAAVDCG*GAETSGTPRGGPEGGGAAPPGAGL DPTAAAGRPGHAGAGATGRHPQRLPGPRPQHARG PLGDALPPRTWTCSHRPGTAPSLHPGLRAPLPC WPQPCWGSPPGQEQARVVPVPPQENSRSVNGNM PPADT

Sequence MSLN.bAug05

GGAGGCTGCCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCC CGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCCTGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCT GTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCTCGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGG CAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTCTCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCG GCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCAAGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTC TGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCCAGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCC CGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGGCTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTC TGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGCGCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGT GAGCTGCCCGGGACCCCTGGACCAGGACCAGCAGGAGGCAGCCAGGGCGGCTCTGCAGGGCGGGGGACCCCCCTACGGCCCCCCGTCGACATGGTCTGTC TCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATCCGCAGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCT CTCGGGACCCATCCTGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGCGGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCG CGAGATAGACGAGAGCCTCATCTTCTACAAGAAGTGGGAGCTGGAAGCCTGCGTGGATGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATC CCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTACCCACAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCT TCCTCAAGATGAGCCCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCCTGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCC TCAGGTGGCCACCCTGATCGACCGCTTTGTGAAGGGAAGGGGCCAGCTAGACAAAGACACCCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGC TCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCCCCCAGCAGCATCTGGGCGGTCAGGCCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCC TCTATCCCAAGGCCCGCCTTGCTTTCCAGAACATGAACGGGTCCGAATACTTCGTGAAGATCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAA GGCGCTCAGTCAGCAGAATGTGAGCATGGACTTGGCCACGTTCATGAAGCTGCGGACGGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTT CTGGGACCCCACGTGGAGGGCCTGAAGGCGGAGGAGCGGCACCGCCCGGTGCGGGACTGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGC TGGGGCTACAGGGCGGCATCCCCAACGGCTACCTGGTCCTAGACCTCAGCATGCAAGAGGCCCTCTCGGGGACGCCCTGCCTCCTAGGACCTGGACCTGT TCTCACCGTCCTGGCACTGCTCCTAGCCTCCACCCTGGCCTGAGGGCCCCACTCCCTTGCTGGCCCCAGCCCTGCTGGGGATCCCCGCCTGGCCAGGAGC AGGCACGGGTGGTCCCCGTTCCACCCCAAGAGAACTCGCGCTCAGTAAACGGGAACATGCCCCCTGCAGACACGT

MSLN.F11 (forward primer)
MSLN.R5 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

GGCQALHPHFPIEETEAEEAQRHALLFLPGRPL PSAVTRGQRATGGPTVPPSLGSTQTMALPTARPL LGSCGTPALGSLLFLLFSLGWVQPSRTLAGETG QEAAPLDGVLANPPNISSLSPRQLLGFPCAEVS GLSTERVRELAVALAQKNVKLSTEQLRCLAHRLS EPPEDLDALPLDLLLFLNPDAFSGPQACTRFFS RITKANVDLLPRGAPERQRLLPAALACWGVRGS LLSEADVRALGGLACDLPGRFVAESAEVLLPRLV SCPGPLDQDQQEAARAALQGGGPPYGPPSTWSV STMDALRGLLPVLGQPIIRSIPQGIVAAWRQRS SRDPSWRQPERTILRPRFRREVEKTACPSGKKAR EIDESLIFYKKWELEACVDAALLATQMDRVNAI PFTYEQLDVLKHKLDELYPQGYPESVIQHLGYL FLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSP QVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLC SLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDV LYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLK ALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKL LGPHVEGLKAEERHRPVRDWILRQRQDDLDTLG LGLQGGIPNGYLVLDLSMQEALSGTPCLLGPGPV LTVLALLLASTLA*GPHSLAGPSPAGDPRLARS RHGWSPFHPKRTRAQ*TGTCPLQTR

Frame 1

EAARLSTPTSQLRKPRQRRLSATHSSFCLAGHS RLL*RADRELPVDPRCLPPWDLHRPWPCQRLDP CWGPVGPPPSAASCSCSSASDGCSPRGPWLERQG RRLRPWTESWPTHLTFPASPLANSLASRVRRCP A*ARSVSGSWLWPWHRRMSSSQQSSCAVWLTGS LSPPRTWTPSHWTCCYSSTQMRSRGPRPAPVSSP ASRRPMWTCSRGGLPSDSGCCLRLWPAGVCGGL C*ARLMCGLWEAWLATCLGALWPSRPKCCYPGW *AARDPWTRTSRRQPGRLCRAGDPPTAPRRHGLS PRWTLCGACCPCWASPSSAASRRASWPRGGNAP LGTHPGGSLNGPSSGRGSGGKWRRQPVLQARRP AR*TRASSSTRSGSWKPAWMRPCWPPRWTA*TPS PSPTSSWTS*SINWMSSTHKVTPSL*SSTWATS SSR*ALRTFASGM*RPWRP*RLCLKSTKGTK*V LRWPP*STAL*REGAS*TKTP*TP*PPSTLGTCA PSAPRS*APCPPAASGRSGPRTWTRVTQGSWTS SIPRPALLSRT*TGPNTS*RSSPSWVGPPRRI* RRSVSRM*AWTWPRS*SCGRMRCCR*LWLRCRNF WDPTWRA*RRRSGTARCGTGSYGSGRTTWTRWG WGYRAASPTATWS*TSACKRPSRGRPAS*DLDL FSPSWHCS*PPPWPEGPTPLLAPALLGIPAWPGA GTGGPRSTPRELALSKREHAPCRH

Frame 2

RLPGSPPPLPN*GNRGRGGSAPRTPLSAWPATP VCCDARTESYRWTHGASLPGIYTDHGLANGSTP VGVLWDPRPRQPPVPALQPRMGAALEDPGWRDR AGGCAPGRSPGQPT*HFQPLPSPTPWLPVCGGVR PEHGACPGAGCGLGTEECQALNRAAALSGSPAL *APRGPGRPPIGPAAIPQPRCVLGAPGLHPFLL PHHEGQCGPAPEGGSRATAAAACGSGLLGCAGVS AERG*CAGSGRPGLRPAWALCGRVGRSAATPAG ELPGTPGPGPAGGSQGGSAGRGTPLRPPVDMVC LHDGRSAGPAARAGPAHHPQHPAGHRGRVAATLL SGPILAAA*TDHPPAAVPAGSGEDSLSFRQEGP RDRREPHLLQEVGAGSLRGCGPAGHPDGPRERH PLHLRAAGRPKA*TG*ALPTRLPRVCDPAPGLPL PQDEP*GHSQVECDVPGDPEGFA*SQQRARNES SGGHPDRPLCEGKGPARQRHPRHPDRLLPWVPV LPQPRGAELRAPQQHLGGQAPGPGHV*PKAAGRP LSQGPPCFPEHERVRILREDPVLPGWGPHGGFE GAQSAECEHGLGHVHEAADGCGAAVDCG*GAET SGTPRGGPEGGGAAPPGAGLDPTAAAGRPGHAGA GATGRHPQRLPGPRPQHARGPLGDALPPRTWTC SHRPGTAPSLHPGLRAPLPCWPQPCWGSPPGQE QARVVPVPPQENSRSVNGNMPPADT

Sequence MSLN.aAug05

CCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGGTGTGGCCAATCACCCTGCACATCAGAGTTACCCTGGGCAGGGCC CACTGAGACCTGGGAGGGGCCACTCGGGACCTGGAGGGCTGGGGGCTGCCCGGGCGTTAGGGGTAAAGCTCCCTACCCAACTGCGCAGAAGGCCTCAGAG GCCTGGGGGCTGGGCTTCCCCTTTCACATCGCCCTTTAGAGGCCCACGTGTGGGCATTGGCCCGCGATCTGAAAGGGGCTGTCCTGTTCCTCATGGGCGC TGCCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCCCGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCC TGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCT CGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTC TCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCA AGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCC AGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGG CTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGC GCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAGGAGGCAGCCAGGGCGGCTCTGCA GGGCGGGGGACCCCCCTACGGCCCCCCGTCGACATGGTCTGTCTCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATCCGC AGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGC GGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTACAAGAAGTGGGAGCTGGAAGCCTGCGTGGA TGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATCCCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTACCCA CAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGCCCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCC TGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTTGTGAAGGGAAGGGGCCAGCTAGACAAAGA CACCCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCCCCCAGCAGCATCTGGGCGGTCAGG CCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCCCGCCTTGCTTTCCAGAACATGAACGGGTCCGAATACTTCGTGA AGATCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATGGACTTGGCCACGTTCATGAAGCTGCGGAC GGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTTCTGGGACCCCACGTGGAGGGCCTGAAGGCGGAGGAGCGGCACCGCCCGGTGCGGGAC TGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGCGGCATCCCCAACGGCTACCTGGTCCTAGACCTCAGCATGCAAG AGGCCCTCTCGGGGACGCCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACCCTGGCCTGAGGGCCCCACTCCCT TGCTGGCCCCAGCCCTGCTGGGGATCCCCGCCTGGCCAGGAGCAGGCACGGGTGGTCCCCGTTCCACCCCAAGAGAACTCGCGCTCAGTAAACGGGAACA TGCCCCCTGCAGACACGT

MSLN.F11 (forward primer)
MSLN.R5 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

PGSPPPLPN*GNRGRGGSGVANHPAHQSYPGQG PLRPGRGHSGPGGLGAARALGVKLPTQLRRRPQR PGGWASPFTSPFRGPRVGIGPRSERGCPVPHGR CQRHALLFLPGRPLPSAVTRGQRATGGPTVPPS LGSTQTMALPTARPLLGSCGTPALGSLLFLLFSL GWVQPSRTLAGETGQEAAPLDGVLANPPNISSL SPRQLLGFPCAEVSGLSTERVRELAVALAQKNV KLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNP DAFSGPQACTRFFSRITKANVDLLPRGAPERQR LLPAALACWGVRGSLLSEADVRALGGLACDLPG RFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQ GGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIR SIPQGIVAAWRQRSSRDPSWRQPERTILRPRFR REVEKTACPSGKKAREIDESLIFYKKWELEACVD AALLATQMDRVNAIPFTYEQLDVLKHKLDELYP QGYPESVIQHLGYLFLKMSPEDIRKWNVTSLET LKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKD TLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVR PQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFV KIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRT DAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRD WILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQ EALSGTPCLLGPGPVLTVLALLLASTLA*GPHSL AGPSPAGDPRLARSRHGWSPFHPKRTRAQ*TGT CPLQTR

Frame 1

QALHPHFPIEETEAEEAQVWPITLHIRVTLGRA H*DLGGATRDLEGWGLPGR*G*SSLPNCAEGLR GLGAGLPLSHRPLEAHVWALARDLKGAVLFLMGA ASATHSSFCLAGHSRLL*RADRELPVDPRCLPP WDLHRPWPCQRLDPCWGPVGPPPSAASCSCSSA SDGCSPRGPWLERQGRRLRPWTESWPTHLTFPAS PLANSLASRVRRCPA*ARSVSGSWLWPWHRRMS SSQQSSCAVWLTGSLSPPRTWTPSHWTCCYSST QMRSRGPRPAPVSSPASRRPMWTCSRGGLPSDSG CCLRLWPAGVCGGLC*ARLMCGLWEAWLATCLG ALWPSRPKCCYPGW*AARDPWTRTSRRQPGRLC RAGDPPTAPRRHGLSPRWTLCGACCPCWASPSSA ASRRASWPRGGNAPLGTHPGGSLNGPSSGRGSG GKWRRQPVLQARRPAR*TRASSSTRSGSWKPAW MRPCWPPRWTA*TPSPSPTSSWTS*SINWMSSTH KVTPSL*SSTWATSSSR*ALRTFASGM*RPWRP *RLCLKSTKGTK*VLRWPP*STAL*REGAS*TK TP*TP*PPSTLGTCAPSAPRS*APCPPAASGRSG PRTWTRVTQGSWTSSIPRPALLSRT*TGPNTS* RSSPSWVGPPRRI*RRSVSRM*AWTWPRS*SCG RMRCCR*LWLRCRNFWDPTWRA*RRRSGTARCGT GSYGSGRTTWTRWGWGYRAASPTATWS*TSACK RPSRGRPAS*DLDLFSPSWHCS*PPPWPEGPTP LLAPALLGIPAWPGAGTGGPRSTPRELALSKREH APCRH

Frame 2

RLSTPTSQLRKPRQRRLRCGQSPCTSELPWAGP TETWEGPLGTWRAGGCPGVRGKAPYPTAQKASE AWGLGFPFHIAL*RPTCGHWPAI*KGLSCSSWA LPAPRTPLSAWPATPVCCDARTESYRWTHGASLP GIYTDHGLANGSTPVGVLWDPRPRQPPVPALQP RMGAALEDPGWRDRAGGCAPGRSPGQPT*HFQP LPSPTPWLPVCGGVRPEHGACPGAGCGLGTEECQ ALNRAAALSGSPAL*APRGPGRPPIGPAAIPQP RCVLGAPGLHPFLLPHHEGQCGPAPEGGSRATA AAACGSGLLGCAGVSAERG*CAGSGRPGLRPAWA LCGRVGRSAATPAGELPGTPGPGPAGGSQGGSA GRGTPLRPPVDMVCLHDGRSAGPAARAGPAHHP QHPAGHRGRVAATLLSGPILAAA*TDHPPAAVPA GSGEDSLSFRQEGPRDRREPHLLQEVGAGSLRG CGPAGHPDGPRERHPLHLRAAGRPKA*TG*ALP TRLPRVCDPAPGLPLPQDEP*GHSQVECDVPGDP EGFA*SQQRARNESSGGHPDRPLCEGKGPARQR HPRHPDRLLPWVPVLPQPRGAELRAPQQHLGGQ APGPGHV*PKAAGRPLSQGPPCFPEHERVRILRE DPVLPGWGPHGGFEGAQSAECEHGLGHVHEAAD GCGAAVDCG*GAETSGTPRGGPEGGGAAPPGAG LDPTAAAGRPGHAGAGATGRHPQRLPGPRPQHAR GPLGDALPPRTWTCSHRPGTAPSLHPGLRAPLP CWPQPCWGSPPGQEQARVVPVPPQENSRSVNGN MPPADT