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Sequences

Sequence MSLN.cAug05

CCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGGTGTGGCCAATCACCCTGCACATCAGAGTTACCCTGGGCAGGGCC
CACTGAGACCTGGGAGGGGCCACTCGGGACCTGGAGGGCTGGGGGCTGCCCGGGCGTTAGGGGTAAAGCTCCCTACCCAACTGCGCAGAAGGCCTCAGAG
GCCTGGGGGCTGGGCTTCCCCTTTCACATCGCCCTTTAGAGGCCCACGTGTGGGCATTGGCCCGCGATCTGAAAGGGGCTGTCCTGTTCCTCATGGGCGC
TGCCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCCCGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCC
TGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCT
CGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGCCTC
TCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCA
AGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCTGCTGCTATTCCTCAACCC
AGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAGCGG
CTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGC
GCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTGGCGGCAGCCTGA
ACGGACCATCCTCCGGCCGCGGTTCCGGCGGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTAC
AAGAAGTGGGAGCTGGAAGCCTGCGTGGATGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAACGCCATCCCCTTCACCTACGAGCAGCTGGACGTCC
TAAAGCATAAACTGGATGAGCTCTACCCACAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGCCCTGAGGACATTCG
CAAGTGGAATGTGACGTCCCTGGAGACCCTGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTT
GTGAAGGGAAGGGGCCAGCTAGACAAAGACACCCTAGACACCCTGACCGCCTTCTACCCTGGGTACCTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCG
TGCCCCCCAGCAGCATCTGGGCGGTCAGGCCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCCCGCCTTGCTTTCCA
GAACATGAACGGGTCCGAATACTTCGTGAAGATCCAGTCCTTCCTGGGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATG
GACTTGGCCACGTTCATGAAGCTGCGGACGGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAGAAACTTCTGGGACCCCACGTGGAGGGCCTGAAGG
CGGAGGAGCGGCACCGCCCGGTGCGGGACTGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGCGGCATCCCCAACGG
CTACCTGGTCCTAGACCTCAGCATGCAAGGTGGGCGGGGCGGCCAGGCCAGGGCTGGGGGCAGAGCTGGGGGCGTGGAGGTGGGCGCTCTGAGTCACCCC
TCTCTCTGTAGAGGCCCTCTCGGGGACGCCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACCCTGGCCTGAGGG
CCCCACTCCCTTGCTGGCCCCAGCCCTGCTGGGGATCCCCGCCTGGCCAGGAGCAGGCACGGGTGGTCCCCGTTCCACCCCAAGAGAACTCGCGCTCAGT
AAACGGGAACATGCCCCCTGCAGACACGT
  • MSLN.F11 (forward primer)
  • MSLN.R5 (reverse primer)
  • Alternative donor

Frame 0

PGSPPPLPN*GNRGRGGSGVANHPAHQSYPGQG
PLRPGRGHSGPGGLGAARALGVKLPTQLRRRPQR
PGGWASPFTSPFRGPRVGIGPRSERGCPVPHGR
CQRHALLFLPGRPLPSAVTRGQRATGGPTVPPS
LGSTQTMALPTARPLLGSCGTPALGSLLFLLFSL
GWVQPSRTLAGETGQEAAPLDGVLANPPNISSL
SPRQLLGFPCAEVSGLSTERVRELAVALAQKNV
KLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNP
DAFSGPQACTRFFSRITKANVDLLPRGAPERQR
LLPAALACWGVRGSLLSEADVRALGGLACDLPG
RFVAESAEVLLPRLGIVAAWRQRSSRDPSWRQPE
RTILRPRFRREVEKTACPSGKKAREIDESLIFY
KKWELEACVDAALLATQMDRVNAIPFTYEQLDV
LKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIR
KWNVTSLETLKALLEVNKGHEMSPQVATLIDRF
VKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSS
VPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQ
NMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSM
DLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLK
AEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNG
YLVLDLSMQGGRGGQARAGGRAGGVEVGALSHP
SLCRGPLGDALPPRTWTCSHRPGTAPSLHPGLR
APLPCWPQPCWGSPPGQEQARVVPVPPQENSRSV
NGNMPPADT

Frame 1

QALHPHFPIEETEAEEAQVWPITLHIRVTLGRA
H*DLGGATRDLEGWGLPGR*G*SSLPNCAEGLR
GLGAGLPLSHRPLEAHVWALARDLKGAVLFLMGA
ASATHSSFCLAGHSRLL*RADRELPVDPRCLPP
WDLHRPWPCQRLDPCWGPVGPPPSAASCSCSSA
SDGCSPRGPWLERQGRRLRPWTESWPTHLTFPAS
PLANSLASRVRRCPA*ARSVSGSWLWPWHRRMS
SSQQSSCAVWLTGSLSPPRTWTPSHWTCCYSST
QMRSRGPRPAPVSSPASRRPMWTCSRGGLPSDSG
CCLRLWPAGVCGGLC*ARLMCGLWEAWLATCLG
ALWPSRPKCCYPGWASWPRGGNAPLGTHPGGSL
NGPSSGRGSGGKWRRQPVLQARRPAR*TRASSST
RSGSWKPAWMRPCWPPRWTA*TPSPSPTSSWTS
*SINWMSSTHKVTPSL*SSTWATSSSR*ALRTF
ASGM*RPWRP*RLCLKSTKGTK*VLRWPP*STAL
*REGAS*TKTP*TP*PPSTLGTCAPSAPRS*AP
CPPAASGRSGPRTWTRVTQGSWTSSIPRPALLS
RT*TGPNTS*RSSPSWVGPPRRI*RRSVSRM*AW
TWPRS*SCGRMRCCR*LWLRCRNFWDPTWRA*R
RRSGTARCGTGSYGSGRTTWTRWGWGYRAASPT
ATWS*TSACKVGGAARPGLGAELGAWRWAL*VTP
LSVEALSGTPCLLGPGPVLTVLALLLASTLA*G
PHSLAGPSPAGDPRLARSRHGWSPFHPKRTRAQ
*TGTCPLQTR

Frame 2

RLSTPTSQLRKPRQRRLRCGQSPCTSELPWAGP
TETWEGPLGTWRAGGCPGVRGKAPYPTAQKASE
AWGLGFPFHIAL*RPTCGHWPAI*KGLSCSSWA
LPAPRTPLSAWPATPVCCDARTESYRWTHGASLP
GIYTDHGLANGSTPVGVLWDPRPRQPPVPALQP
RMGAALEDPGWRDRAGGCAPGRSPGQPT*HFQP
LPSPTPWLPVCGGVRPEHGACPGAGCGLGTEECQ
ALNRAAALSGSPAL*APRGPGRPPIGPAAIPQP
RCVLGAPGLHPFLLPHHEGQCGPAPEGGSRATA
AAACGSGLLGCAGVSAERG*CAGSGRPGLRPAWA
LCGRVGRSAATPAGHRGRVAATLLSGPILAAA*
TDHPPAAVPAGSGEDSLSFRQEGPRDRREPHLL
QEVGAGSLRGCGPAGHPDGPRERHPLHLRAAGRP
KA*TG*ALPTRLPRVCDPAPGLPLPQDEP*GHS
QVECDVPGDPEGFA*SQQRARNESSGGHPDRPL
CEGKGPARQRHPRHPDRLLPWVPVLPQPRGAELR
APQQHLGGQAPGPGHV*PKAAGRPLSQGPPCFP
EHERVRILREDPVLPGWGPHGGFEGAQSAECEH
GLGHVHEAADGCGAAVDCG*GAETSGTPRGGPEG
GGAAPPGAGLDPTAAAGRPGHAGAGATGRHPQR
LPGPRPQHARWAGRPGQGWGQSWGRGGGRSESP
LSL*RPSRGRPAS*DLDLFSPSWHCS*PPPWPEG
PTPLLAPALLGIPAWPGAGTGGPRSTPRELALS
KREHAPCRH

Sequence MSLN.gAug05

CCAGGCTCTCCACCCCCACTTCCCAATTGAGGAAACCGAGGCAGAGGAGGCTCAGGTGTGGCCAATCACCCTGCACATCAGAGTTACCCTGGGCAGGGCC
CACTGAGACCTGGGAGGGGCCACTCGGGACCTGGAGGGCTGGGGGCTGCCCGGGCGTTAGGGGTAAAGCTCCCTACCCAACTGCGCAGAAGGCCTCAGAG
GCCTGGGGGCTGGGCTTCCCCTTTCACATCGCCCTTTAGAGGCCCACGTGTGGGCATTGGCCCGCGATCTGAAAGGGGCTGTCCTGTTCCTCATGGGCGC
TGCCAGCGCCACGCACTCCTCTTTCTGCCTGGCCGGCCACTCCCGTCTGCTGTGACGCGCGGACAGAGAGCTACCGGTGGACCCACGGTGCCTCCCTCCC
TGGGATCTACACAGACCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCT
CGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAGACAGGGCAGCAGGCCCTGTGTCCCCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCA
CCTAACATTTCCAGCCTCTCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCT
TGGCACAGAAGAATGTCAAGCTCTCAACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTGGACCT
GCTGCTATTCCTCAACCCAGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGG
GCTCCCGAGCGACAGCGGCTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGG
CTTGCGACCTGCCTGGGCGCTTTGTGGCCGAGTCGGCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAGGAGGC
AGCCAGGGCGGCTCTGCAGGGCGGGGGACCCCCCTACGGGTAAGTGAAGGTGTCTGGAACCTCGAAGGCTCACCTGGCGGCGTGGTATCAGCAGCGTGAG
GACACTTGCGGCCATGCCTCAAGGCCCAGGGTCAGTCACCCCCGCCACCACCCCTGCCAATGCCCCCAGCGTCCCCTCCACAGCCAGGGGGTACGGCCTG
GCCTCTTCCCTCTCTGGGGCTGCACGTGGGGGGTCCCTGAGCTGTGTCCCGTGTCTGCACAGCCCCCCGTCGACATGGTCTGTCTCCACGATGGACGCTC
TGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATCCGCAGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCCTG
GCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGCGGGAAGTGGAGAAGACAGCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGC
CTCATCTTCTACAAGAAGTGG
  • MSLN.F11 (forward primer)
  • MSLN.R5 (reverse primer)
  • Alternative donor

Frame 0

PGSPPPLPN*GNRGRGGSGVANHPAHQSYPGQG
PLRPGRGHSGPGGLGAARALGVKLPTQLRRRPQR
PGGWASPFTSPFRGPRVGIGPRSERGCPVPHGR
CQRHALLFLPGRPLPSAVTRGQRATGGPTVPPS
LGSTQTMALPTARPLLGSCGTPALGSLLFLLFSL
GWVQPSRTLAGETGQQALCPQEAAPLDGVLANP
PNISSLSPRQLLGFPCAEVSGLSTERVRELAVA
LAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDL
LLFLNPDAFSGPQACTRFFSRITKANVDLLPRG
APERQRLLPAALACWGVRGSLLSEADVRALGGL
ACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEA
ARAALQGGGPPYG*VKVSGTSKAHLAAWYQQRE
DTCGHASRPRVSHPRHHPCQCPQRPLHSQGVRP
GLFPLWGCTWGVPELCPVSAQPPVDMVCLHDGRS
AGPAARAGPAHHPQHPAGHRGRVAATLLSGPIL
AAA*TDHPPAAVPAGSGEDSLSFRQEGPRDRRE
PHLLQEV

Frame 1

QALHPHFPIEETEAEEAQVWPITLHIRVTLGRA
H*DLGGATRDLEGWGLPGR*G*SSLPNCAEGLR
GLGAGLPLSHRPLEAHVWALARDLKGAVLFLMGA
ASATHSSFCLAGHSRLL*RADRELPVDPRCLPP
WDLHRPWPCQRLDPCWGPVGPPPSAASCSCSSA
SDGCSPRGPWLERQGSRPCVPRRLRPWTESWPTH
LTFPASPLANSLASRVRRCPA*ARSVSGSWLWP
WHRRMSSSQQSSCAVWLTGSLSPPRTWTPSHWT
CCYSSTQMRSRGPRPAPVSSPASRRPMWTCSRGG
LPSDSGCCLRLWPAGVCGGLC*ARLMCGLWEAW
LATCLGALWPSRPKCCYPGW*AARDPWTRTSRR
QPGRLCRAGDPPTGK*RCLEPRRLTWRRGISSVR
TLAAMPQGPGSVTPATTPANAPSVPSTARGYGL
ASSLSGAARGGSLSCVPCLHSPPSTWSVSTMDA
LRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSW
RQPERTILRPRFRREVEKTACPSGKKAREIDES
LIFYKKW

Frame 2

RLSTPTSQLRKPRQRRLRCGQSPCTSELPWAGP
TETWEGPLGTWRAGGCPGVRGKAPYPTAQKASE
AWGLGFPFHIAL*RPTCGHWPAI*KGLSCSSWA
LPAPRTPLSAWPATPVCCDARTESYRWTHGASLP
GIYTDHGLANGSTPVGVLWDPRPRQPPVPALQP
RMGAALEDPGWRDRAAGPVSPGGCAPGRSPGQP
T*HFQPLPSPTPWLPVCGGVRPEHGACPGAGCGL
GTEECQALNRAAALSGSPAL*APRGPGRPPIGP
AAIPQPRCVLGAPGLHPFLLPHHEGQCGPAPEG
GSRATAAAACGSGLLGCAGVSAERG*CAGSGRPG
LRPAWALCGRVGRSAATPAGELPGTPGPGPAGG
SQGGSAGRGTPLRVSEGVWNLEGSPGGVVSAA*
GHLRPCLKAQGQSPPPPPLPMPPASPPQPGGTAW
PLPSLGLHVGGP*AVSRVCTAPRRHGLSPRWTL
CGACCPCWASPSSAASRRASWPRGGNAPLGTHP
GGSLNGPSSGRGSGGKWRRQPVLQARRPAR*TRA
SSSTRS

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