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Sequences

Sequence RNASET2.vaAug05

RNASET2.F13 (forward primer)
RNASET2.R15 (reverse primer)
Multiple exon skipping

Frame 0

RRAARWPCPLASGGGA*RPRG*RWTPSLGERRT TERRNLGSALGAGLRGAGGHARGGASAGPPAGNP LAAVA*GASFEAGER*GGGALACGERTPPGLHR PRPHPIAAQDRRSHIIDVVKAH*PKAGRTAVWS LIPTVVVVTHTCFSSETALPAAGAPTPAPF*SS* SGSA*IVPSFKKKMKYELHFDK*RGCEGQKEQK LPAEMLR*KAQSASWIEKTRPIRVLTSKDSSHT **HP*AQSKGLDKYLLCKWK

Frame 1

DAQRAGPAPWRREAGHRDLGVDAGHRALGSAGP LSAET*GRRWGRGCGARVGTQGAGRRRGLQPAT RWLPSPEARALKPASGEEEAPSRVESGLRLDSTA HGRIPSPHKIAGVILST*LKPTSLKPGGLRCGP *SLRWWW*LTLVSVLKLLFLLLEPPPLLHFEVA DPDQLRLCQVSRKR*NMSCILTNEEDVKGRRSRN YQQRC*DERHRVQVGLKKQDLSEC*LQKTHLIH NDTHRLKVKDWINIYYANGK

Frame 2

TRSALALPPGVGRRGIETSGLTLDTEPWGAPDH *APKLRVGAGGGAAGRGWARKGRGVGGASSRQP VGCRRLRREL*SRRAVRRRRPRVWRADSAWTPP PTAASHRRTRSPESYYRRS*SPLA*SRADCGVVP DPYGGGGNSHLFQF*NCSSCCWSPHPCSILK*L IRISLDCAKFQEKDEI*VAF*QMKRM*RAEGAE TTSRDAKMKGTECKLD*KNKTYPSADFKRLISYI MTPIGSK*RIG*IFIMQMEN

Sequence RNASET2.mAug05

CGACGCGCAGCGCGCTGGCCCTGCCCCCTGGCGTCGGGAGGCGGGGCATAGAGACCTCGGGGTTGACGCTGGACACCGAGCCTTGGGGAGCGCCGGACCA CTGAGCGCCGAAACTTAGGGTCGGCGCTGGGGGCGGGGCTGCGGGGCGCGGGTGGGCACGCAAGGGGCGGGGCGTCGGCGGGGCCTCCAGCCGGCAACCC GTTGGCTGCCGTCGCCTGAGGCGCGAGCTTTGAAGCCGGCGAGCGGTGAGGAGGAGGCGCCCTCGCGTGTGGAGAGCGGACTCCGCCTGGACTCCACCGC CCACGGCCGCATCCCATCGCCGCACAAGATCGCCGGAGTCATATTATCGACGTAGTTAAAGCCCACTAGCCTAAAGCCGGGCGGACTGCGGCTCACTGCA AACTCCGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCTTCTCCAGTAGCTGGAACTACAGACACCAAGAAACTGGACACCCTCCACCGGTAACATAT TTTGGCGAGCCAGCCAGGAGAAGGTGTGGTCCCTGATCCCTACGGTGGTGGTGGTAACTCACACTTGTTTCAGTGACAACCATGAGTGGAAAAAACTAAT TATGGTTCAGCACTGGCCTGAGACAGTATGCGAGAAAATTCAAAACGACTGTAGAGACCCTCCGGATTACTGGACAATACATGGACTATGGCCCGATAAA AGTGAAGGATGTAATAGATCGTGGCCCTTCAATTTAGAAGAGATTAAGGATCTTTTGCCAGAAATGAGGGCATACTGGCCTGACGTAATTCACTCGTTTC CCAATCGCAGCCGCTTCTGGAAGCATGAGTGGGAAAAGCATGGGACCTGCGCCGCCCAGGTGGATGCGCTCAACTCCCAGAAGAAGTACTTTGGCAGAAG CCTGGAACTCTACAGGGAGCTGGACCTCAACAGGTTGCAGATTTTAAAGATGCCCTTGCCAGAGTATATGGAGTGATACCCAAAATCCAGTGCCTTCCAC CAAGCCAGGATGAGGAAGTACAGACAATTGGTCAGATAGAACTGTGCCTCACTAAGCAAGACCAGCAGCTGCAAAACTGCACCGAGCCGGGGGAGCAGCC GTCCCCCAAGCAGGAAGTCTGGCTGGCAAATGGGGCCGCCGAGAGCCGGGGTCTGAGAGTCTGTGAAGATGGCCCAGTCTTCTATCCCCCACCTAAAAAG ACCAAGCATTGATGCCCAAGTTTTGGAAATATTCTGTTTTAAAAAGCAAGAGAAATTCACAAACTGCAGCTTTCTAAAAAACAACTCCAAAGTGAAGTCT GTTTATTTTGCTGTTTCCCCTCCATGCCTGTGAATTGGATGTTGTGGTCCCTGTAGAGTGAGTGCATGTGGCGTACACGGGGAGAAAGGAGCTCTCCCAC GCCTGAGTGGCTGTTTTGTGCTTGGGATAAAGCTCACAGATTGTTTTTTTTTTTCTCTATTAAACTCTTCAGTGCCCTGGTAGATCAGGCAGGCATACTT GGAATTTAGACAGATGGCACCGATTCAGGGGACTCTCTTGCTCGAGTTTTCCCCTCGGAGCCTGTTGCCTTCTTGGCGCAGGGACCCTGTCAAGACGAAG AGCTCCACGCATGGCAGGAGCCCACGCTGATGCCTGGTGGGCCTGGGGGGTGTCTCTAGCTCAGACTGGGTCTGTTCCATGTGACCAGAGCCCCAGGACA TCGTTCCTTAGCCAGCTGCTCCGTTGTTGGAGGCCTGGTGTGGTCACAGGTGTGTCCTAGAACGGTGACTTTGCCCCTCCTGCCCTAGGAGATTTGGGTG TATTCACAGAGCAGCCATCTCCTGTCTCCGTCAGAGCAGTCTCTTCCAGGTTACTGGAAGGTCAGTTTCTGTCAGGCCTCACTTCCTTCTTATCAGAGGG TAGCCAGGTCCCTGCACTGGTATCTATTTAGAAATGTACATGGAGCCCCCAGCGAAATGTGCCTACATTGTGTGTTCCAGAACCGGAGGTACCCTTACCT TCTAGATATGTGATGACATGGCTCTCACGTCAGGCAGACTGCTGCACCCACGCTCTCGGCCTGCCGTGCAGGGATTTCACTCGTGGAGTGAGGCATCTGG GGGCTGTGCAGTGAGGAGCTTTCCATGTGTTGTTAAGACAACCTGTTAGCATTGTCTTCTCCCGCGTCTCCTGGCCTCTTCTCTCTTTAAATCCTTTTCT CTGTTTGTCTTAATCACATTAACGTCCTATTTATGTTTTATTCAATATACATTTAAAATAGAACTTACTATGTGCCAGGCATTGTGTTCAACACGTGAAA TATTTAATCCTTAGAATAACCATATTGTCAGCATAATTTACAA

RNASET2.F13 (forward primer)
RNASET2.R15 (reverse primer)
Multiple exon skipping

Frame 0

RRAARWPCPLASGGGA*RPRG*RWTPSLGERRT TERRNLGSALGAGLRGAGGHARGGASAGPPAGNP LAAVA*GASFEAGER*GGGALACGERTPPGLHR PRPHPIAAQDRRSHIIDVVKAH*PKAGRTAAHC KLRLPGSSDSPASASPVAGTTDTKKLDTLHR*HI LASQPGEGVVPDPYGGGGNSHLFQ*QP*VEKTN YGSALA*DSMRENSKRL*RPSGLLDNTWTMAR* K*RM**IVALQFRRD*GSFARNEGILA*RNSLVS QSQPLLEA*VGKAWDLRRPGGCAQLPEEVLWQK PGTLQGAGPQQVADFKDALARVYGVIPKIQCLP PSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQP SPKQEVWLANGAAESRGLRVCEDGPVFYPPPKK TKH*CPSFGNILF*KAREIHKLQLSKKQLQSEV CLFCCFPSMPVNWMLWSL*SECMWRTRGERSSPT PEWLFCAWDKAHRLFFFFSIKLFSALVDQAGIL GI*TDGTDSGDSLARVFPSEPVAFLAQGPCQDE ELHAWQEPTLMPGGPGGCL*LRLGLFHVTRAPGH RSLASCSVVGGLVWSQVCPRTVTLPLLP*EIWV YSQSSHLLSPSEQSLPGYWKVSFCQASLPSYQR VARSLHWYLFRNVHGAPSEMCLHCVFQNRRYPYL LDM**HGSHVRQTAAPTLSACRAGISLVE*GIW GLCSEELSMCC*DNLLALSSPASPGLFSL*ILF SVCLNHINVLFMFYSIYI*NRTYYVPGIVFNT*N I*SLE*PYCQHNLQ

Frame 1

DAQRAGPAPWRREAGHRDLGVDAGHRALGSAGP LSAET*GRRWGRGCGARVGTQGAGRRRGLQPAT RWLPSPEARALKPASGEEEAPSRVESGLRLDSTA HGRIPSPHKIAGVILST*LKPTSLKPGGLRLTA NSASRVQVILLPQLLQ*LELQTPRNWTPSTGNI FWRASQEKVWSLIPTVVVVTHTCFSDNHEWKKLI MVQHWPETVCEKIQNDCRDPPDYWTIHGLWPDK SEGCNRSWPFNLEEIKDLLPEMRAYWPDVIHSF PNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRS LELYRELDLNRLQILKMPLPEYME*YPKSSAFH QARMRKYRQLVR*NCASLSKTSSCKTAPSRGSS RPPSRKSGWQMGPPRAGV*ESVKMAQSSIPHLKR PSIDAQVLEIFCFKKQEKFTNCSFLKNNSKVKS VYFAVSPPCL*IGCCGPCRVSACGVHGEKGALP RLSGCFVLGIKLTDCFFFSLLNSSVPW*IRQAYL EFRQMAPIQGTLLLEFSPRSLLPSWRRDPVKTK SSTHGRSPR*CLVGLGGVSSSDWVCSM*PEPQD IVP*PAAPLLEAWCGHRCVLER*LCPSCPRRFGC IHRAAISCLRQSSLFQVTGRSVSVRPHFLLIRG *PGPCTGIYLEMYMEPPAKCAYIVCSRTGGTLT F*ICDDMALTSGRLLHPRSRPAVQGFHSWSEASG GCAVRSFPCVVKTTC*HCLLPRLLASSLFKSFS LFVLITLTSYLCFIQYTFKIELTMCQALCSTRE IFNP*NNHIVSIIY

Frame 2

TRSALALPPGVGRRGIETSGLTLDTEPWGAPDH *APKLRVGAGGGAAGRGWARKGRGVGGASSRQP VGCRRLRREL*SRRAVRRRRPRVWRADSAWTPP PTAASHRRTRSPESYYRRS*SPLA*SRADCGSLQ TPPPGFK*FSCLSFSSSWNYRHQETGHPPPVTY FGEPARRRCGP*SLRWWW*LTLVSVTTMSGKN* LWFSTGLRQYARKFKTTVETLRITGQYMDYGPIK VKDVIDRGPSI*KRLRIFCQK*GHTGLT*FTRF PIAAASGSMSGKSMGPAPPRWMRSTPRRSTLAE AWNSTGSWTSTGCRF*RCPCQSIWSDTQNPVPST KPG*GSTDNWSDRTVPH*ARPAAAKLHRAGGAA VPQAGSLAGKWGRREPGSESL*RWPSLLSPT*K DQALMPKFWKYSVLKSKRNSQTAAF*KTTPK*SL FILLFPLHACELDVVVPVE*VHVAYTGRKELSH A*VAVLCLG*SSQIVFFFLY*TLQCPGRSGRHT WNLDRWHRFRGLSCSSFPLGACCLLGAGTLSRRR APRMAGAHADAWWAWGVSLAQTGSVPCDQSPRT SFLSQLLRCWRPGVVTGVS*NGDFAPPALGDLG VFTEQPSPVSVRAVSSRLLEGQFLSGLTSFLSEG SQVPALVSI*KCTWSPQRNVPTLCVPEPEVPLP SRYVMTWLSRQADCCTHALGLPCRDFTRGVRHL GAVQ*GAFHVLLRQPVSIVFSRVSWPLLSLNPFL CLS*SH*RPIYVLFNIHLK*NLLCARHCVQHVK YLILRITILSA*FT