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Sequences

Sequence RSN.cAug05

CTTGGGAAGAATGATGGCGCTGTTGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACAAAGTTACCAAGATTGGCT TCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGTCCGCCAGCCTGAAGCGCAGCCCTTCTGCCTCTTCCCT CAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAGCAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTACGCCAGGAAGATCTCCGGT ACCACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGGAGAGGGCGGAGGTGGCCAAGGCCACGA GCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGACGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAATGGACCAGCTGCGAACAAT GGTGGAAGCTGCTGACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTCCGGGTTGAAGAAGAATCA ATTACCAAAGGTGATCTTGAGACGCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTTGAACAGAGCCTGCTCTTTGAAAAGACCAAAGCTGACAAAC TCCAGAGGGAGTTAGAAGACACTAGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCATTGAGAGTACAGGAAGTAGC TGAGCTCCGAAGAAGGCTAGAGTCCAATAAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCAAGAAAAGTTAGAA GTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTCATCAGAAGGAGATAAAGGCTCTGTATACCG CCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGCTCTATGGAAGTCCAAACT GGAGACTGCCATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCAGAATTTGCTGAACTAAAA ACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTTGCAGAATCAACAAGACTCTGAACGGGCTGCCCATGCTAAAGAGATGGAAG CCTTGAGGGCTAAACTGATGAAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGAAGACCAGCATCTCGTAGA AATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGAAAGAAAAGTTTGCTGAAGCTTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAA GAAACTGTAAATAAGTTACACCAAAAGGAGGAACAGTTTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAAT TTAGAGAGAAAGATGAGAGAGAAGAGCAGCTGATAAAGGCAAAGGAAAAACTGGAAAATGACATTGCAGAAATAATGAAGATGTCAGGAGATAACTCTTC TCAGCTGACAAAAATGAACGATGAATTACGTCTGAAAGAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTG CAAAAAAGTATTGAGGACATGACTGTCAAAGCTGAACAGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGAATTGGAGAGGAAATTGT CGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCAGTGTCAGGAGCTGAAAGCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGA AATCCTACAGAACCTCCAGAAGACGCTGCTGGACACAGAGGACAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGGAGCTG AGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAGCGGAAGATGCCATGCAGATAATGGAACAGATGACCAAAGAGAAGACTGAGACTCTGGCCTCCT TGGAGGACACCAAGCAAACAAATGCAAAACTACAGAATGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGA ACTCCTGACTGTAGAGAATCAAAAAATGGAAGAATTTAGGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCAGCGTTG CAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGAGAAGCAGGGACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATC AGTTGTTAGAAATGAAAAAAAGAGAATCCAAGTTCATAAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTATAACTAGTGCCTTACT CACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAATGAGGTCACAGTGCTCAGGGGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACT CTAGAGTCTGATAAGGTGAAGCTCGAGCTCAAGGTAAAGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAATACAG ACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAGTCAGTTCATCTGCTGCTGACAACACTTTTCTTCCAATCAGCAAATGATTGATTTCCTAAATTC AGTAATAGTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATGGGAACGGGGATGACCTAAACAAT TATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCTCTTCTGTGACATTTGTGACTGCTTTGATCTCCACGACACAGAGGATTGTCCTA CCCAGGCACAGATGTCAGAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAATCTGTGAGATGTTTGGACACTG GGCCACCAACTGCAATGACGACGAAACCTTCTGATGAAGCCTCCAGTGGAGAACTGGGCTTGCTCAGACGCACTCGCATTGACACAACGTAACACCAGCA TTGTGTGTGCAGACTTCAGGAGAACTCATGTTATTTTTTAACCCCGTCAACAAATCTAGGAAAATATTTTGATCTTCAACAAATTGCCCTTTAGTCTCCC CGTATGAGTTAGAATAATAAATATTTAGTAGGTGAGTTTTCACCTCGAATTTTGTTTTCTTGATTTTTACGTTTGAAGACATTGCACCAGATGCCATTAC ATTTATTGGCCCCCCGACCTTGTAGAAAAACCCCTACCCTCACAATACCTTATTTAAGTAACTTTAAATTATGCCGTTACTTTTCATATTTGCACTAAGA TATTTCCAGGCTGCATTTGTATATTTAGATTTTTTGGTTAAGCTTTGACACTGGAATGAGTTGAAAAAATGTGCCATTTTGCATTTTCATCTACTCATTT AAAGTATTTTATTCTTATTCAAAGAAATATCTGAGCTCTTTGCACTACCTGTTATCAGTAGTGCCTTTACTTCAGGCTTGATAATACTTAGGTGTGATTA TAAAATCATGAAGCAGGTAAAGGGAGGGGCAAGCCCCCAAACTGCTGTGGGGACATTTTATAATCTATATGCTGCACCCACTTAATCTACTGTGGTGTTT TGTTTATTAGTTTTGCATAATTTCAGCTTCTATATATTGTATGTATATATTTTTTAAAAATCTATATTTTGGGAAAAAAACATACACAATGTGTCTTTCT TTTTGGACATTTACCTTTTTGAAAAAGAAAACACTTAAAATGATCATTAGGACATAACAGACTAGGCCAGACATAGCATCTTGTGGCTTTGCAACCATTT TCATTTGTTTGTTTTCCTTTTATTTCTTCACCAGATTTAAATAACTGGAGGAATTTTCTCCAATTTTTTTTTCCTTCTCTGGCAGGTATCCCCAGCAGTC AATTAACAATAAGCCAGTATAAAACACCTAAATAACCAATCTACAATCTCCCTTCACAAGTTTTTTTACTGTTTTTAGATGAATGTACGATGAGAAATTC AACGTTAATAATTCTGGATTTTCTTATCACAAAAAAGAAAATGAAGGACCTCAAAGCACCTGAACAGTTTATCGACCAGTTTGAATCTATTTATCTTCAT TTGAATGTCTTCTAGAATATGTAAAAAGTCATAAAATGTATCTTCCATGCTACATGTACAATAAGAACTTCTATAATTGTATATATGCCTTTGATGTATT TTCCCCTCAAGATTATCAACTGTGTGTTCGACAGTGAATATTCAATCTGGTACCAGTTGAAATTTTTGGTTATAAATGTAATACGAATTGTTTCACAAAC AGAAAACATGTAAAGCAGTATTAAAATTTGGCCAAACAAGTGTTCTGTATCTACTTTAATAAATGGTTATTCTTTTTTGTTT

RSN.F35 (forward primer)
RSN.R13 (reverse primer)
Single exon skipping

Frame 0

LGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIG FPSTTPAKAKANAVRRVMATTSASLKRSPSASSL SSMSSVASSVSSRPSRTGLLTETSSRYARKISG TTALQEALKEKQQHIEQLLAERDLERAEVAKAT SHVGEIEQELALARDGHDQHVLELEAKMDQLRTM VEAADREKVELLNQLEEEKRKVEDLQFRVEEES ITKGDLETQTKLEHARIKELEQSLLFEKTKADK LQRELEDTRVATVSEKSRIMELEKDLALRVQEVA ELRRRLESNKPAGDVDMSLSLLQEISSLQEKLE VTRTDHQREITSLKEHFGAREETHQKEIKALYT ATEKLSKENESLKSKLEHANKENSDVIALWKSKL ETAIASHQQAMEELKVSFSKGLGTETAEFAELK TQIEKMRLDYQHEIENLQNQQDSERAAHAKEME ALRAKLMKVIKEKENSLEAIRSKLDKAEDQHLVE MEDTLNKLQEAEIKKEKFAEASEEAVSVQRSMQ ETVNKLHQKEEQFNMLSSDLEKLRENLADMEAK FREKDEREEQLIKAKEKLENDIAEIMKMSGDNSS QLTKMNDELRLKERDVEELQLKLTKANENASFL QKSIEDMTVKAEQSQQEAAKKHEEEKKELERKL SDLEKKMETSHNQCQELKARYERATSETKTKHEE ILQNLQKTLLDTEDKLKGAREENSGLLQELEEL RKQADKAKAAQTAEDAMQIMEQMTKEKTETLAS LEDTKQTNAKLQNELDTLKENNLKNVEELNKSKE LLTVENQKMEEFRKEIETLKQAAAQKSQQLSAL QEENVKLAEELGRSRDEVTSHQKLEEERSVLNN QLLEMKKRESKFIKDADEEKASLQKSISITSALL TEKDAELEKLRNEVTVLRGENASAKSLHSVVQT LESDKVKLELKVKNLELQLKENKRQLSSSSGNT DTQADEDERAQESQFICC*QHFSSNQQMIDFLNS VIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNN YDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCP TQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHW ATNCNDDETF**SLQWRTGLAQTHSH*HNVTPA LCVQTSGELMLFFNPVNKSRKIF*SSTNCPLVS PYELE**IFSR*VFTSNFVFLIFTFEDIAPDAIT FIGPPTL*KNPYPHNTLFK*L*IMPLLFIFALR YFQAAFVYLDFLVKL*HWNELKKCAILHFHLLI *SILFLFKEISELFALPVISSAFTSGLIILRCDY KIMKQVKGGASPQTAVGTFYNLYAAPT*STVVF CLLVLHNFSFYILYVYIF*KSIFWEKNIHNVSF FLDIYLFEKENT*NDH*DITD*ARHSILWLCNHF HLFVFLLFLHQI*ITGGIFSNFFFLLWQVSPAV N*Q*ASIKHLNNQSTISLHKFFYCF*MNVR*EI QR**FWIFLSQKRK*RTSKHLNSLSTSLNLFIFI *MSSRICKKS*NVSSMLHVQ*ELL*LYICL*CI FPSRLSTVCSTVNIQSGTS*NFWL*M*YELFHK QKTCKAVLKFGQTSVLYLL**MVILFC

Frame 1

LGRMMALLLEQGIFSVNPNMACSLLSTKLPRLA SLPLHQPKPRPTQ*GE*WRPRPPA*SAALLPLP SAP*AQWPPL*AAGPVGQDY*LKPPPVTPGRSPV PLPSRRP*RRSSSTLSSCWRNGIWRGRRWPRPR ATWGR*SRS*LWPGTDMTSMSWNWKPKWTSCEQ WWKLLTGRRWSFSTSLKRRKGRLRTFSSGLKKNQ LPKVILRRRPNWSMPALRSLNRACSLKRPKLTN SRGS*KTLGWLQFQKSHV*WNWRKT*H*EYRK* LSSEEG*SPISLLGMWTCHFPFCKR*ALCKKS*K SPVLTTREK*LL*RSILEPGKKLIRRR*RLCIP PRKSFPKRTSH*KASWSMPTKRTQM**LYGSPN WRLPSHPTSRRWKN*RYLSAKGLEQRRQNLLN*K HK*RK*D*ITNTK*KICRINKTLNGLPMLKRWK P*GLN**KLLKKRKTVWKPSGRNWTKQKTSIS* KWKTR*TNYRKLK*RKKSLLKLQRRQSLFREVCK KL*ISYTKRRNSLTCCLLTWRS*EKT*QIWRQN LERKMREKSS**RQRKNWKMTLQK**RCQEITL LS*QK*TMNYV*KKEM*KNYS*NLQRLMKMQVFC KKVLRT*LSKLNRASKKQLKSMRKKRKNWRGNC RTWKRKWKQATTSVRS*KPGMREPLLRQKPSMK KSYRTSRRRCWTQRTS*RAHGRRTVACCRSWRS* ESKPTKPKLLKQRKMPCR*WNR*PKRRLRLWPP WRTPSKQMQNYRMNWTHLKKTT*KMWKS*TNQK NS*L*RIKKWKNLGKK*KP*SRQQLRSPSSFQRC KKRTLNLLRSWGEAGTKSQVIKSWKKKDLCSII SC*K*KKENPSS*KTQMKRKLPCRNPSV*LVPY SQKRMPSWRN*EMRSQCSGEKTPLPSPCIQLFRL *SLIR*SSSSR*RTWSFNSKKTRGSSAAPQVIQ TLRQTRMKEPRRVSSSAADNTFLPISK*LIS*I Q**WTFKGRIKTSR*RWR*CQKQP*MGTGMT*TI MTVMIRRNSPRRNLASSVTFVTALISTTQRIVL PRHRCQRTLPIPHTMAVGVRNAHTVKSVRCLDT GPPTAMTTKPSDEASSGELGLLRRTRIDTT*HQH CVCRLQENSCYFLTPSTNLGKYFDLQQIAL*SP RMS*NNKYLVGEFSPRILFS*FLRLKTLHQMPL HLLAPRPCRKTPTLTIPYLSNFKLCRYFSYLH*D ISRLHLYI*IFWLSFDTGMS*KNVPFCIFIYSF KVFYSYSKKYLSSLHYLLSVVPLLQA**YLGVI IKS*SR*REGQAPKLLWGHFIIYMLHPLNLLWCF VY*FCIISASIYCMYIFFKNLYFGKKTYTMCLS FWTFTFLKKKTLKMIIRT*QTRPDIASCGFATI FICLFSFYFFTRFK*LEEFSPIFFSFSGRYPQQS INNKPV*NT*ITNLQSPFTSFFTVFR*MYDEKF NVNNSGFSYHKKENEGPQST*TVYRPV*IYLSS FECLLEYVKSHKMYLPCYMYNKNFYNCIYAFDVF SPQDYQLCVRQ*IFNLVPVEIFGYKCNTNCFTN RKHVKQY*NLAKQVFCIYFNKWLFFFV

Frame 2

WEE*WRCCWNKVFSVSTQIWLVRSCPQSYQDWL PFHYTSQSQGQRSEASDGDHVRQPEAQPFCLFP QLHELSGLLCEQQAQSDRTID*NLLPLRQEDLR YHCPPGGPEGEAAAH*AAAGGTGSGEGGGGQGHE PRGGDRAGASSGPGRT*PACPGIGSQNGPAANN GGSC*QGEGGASQPA*RGEKEG*GPSVPG*RRI NYQR*S*DADQTGACPH*GA*TEPAL*KDQS*QT PEGVRRH*GGYSFRKVTYNGTGERPSIESTGSS *APKKARVQ*ACWGCGHVTFPFARDKLFARKVR SHPY*PPERNNFSEGAFWSPGRNSSEGDKGSVYR HGKAFQRERVIEKQAGACQQRELRCDSSMEVQT GDCHRIPPAGDGRTEGIFQQRAWNRDGRIC*TK NTNRENETRLPTRNRKFAESTRL*TGCPC*RDGS LEG*TDESY*RKGKQSGSHQVETGQSRRPASRR NGRHVKQITGS*NKERKVC*SFRGGSLCSEKYA RNCK*VTPKGGTV*HAVF*LGEAERKLSRYGGKI *RER*ERRAADKGKGKTGK*HCRNNEDVRR*LF SADKNER*ITSERKRCRRITAKTYKG**KCKFS AKKY*GHDCQS*TEPARSS*KA*GRKERIGEEIV GPGKENGNKPQPVSGAESQV*ESHF*DKNQA*R NPTEPPEDAAGHRGQAEGRTGGEQWLAAGAGGA EKASRQSQSCSNSGRCHADNGTDDQRED*DSGLL GGHQANKCKTTE*IGHT*RKQLEKCGRAEQIKR TPDCRESKNGRI*ERNRNPKAGSSSEVPAAFSV ARRER*TC*GAGEKQGRSHKSSKAGRRKICAQ*S VVRNEKKRIQVHKRRR*RESFLAEIHQYN*CLT HRKGCRAGETEK*GHSAQGRKRLCQVLAFSCSD SRV**GEARAQGKELGASTQRKQEAAQQLLR*YR HSGRRG*KSPGESVHLLLTTLFFQSAND*FPKF SNSGPSKEESRPQDEGGDDVRSSPEWERG*PKQ L*Q**SGETVQEETSPLL*HL*LL*SPRHRGLSY PGTDVRGPSPFHTPWQSG*GTPIL*NL*DVWTL GHQLQ*RRNLLMKPPVENWACSDALALTQRNTS IVCADFRRTHVIF*PRQQI*ENILIFNKLPFSLP V*VRIINI**VSFHLEFCFLDFYV*RHCTRCHY IYWPPDLVEKPLPSQYLI*VTLNYAVTFHICTK IFPGCICIFRFFG*ALTLE*VEKMCHFAFSSTHL KYFILIQRNI*ALCTTCYQ*CLYFRLDNT*V*L *NHEAGKGRGKPPNCCGDIL*SICCTHLIYCGV LFISFA*FQLLYIVCIYFLKIYILGKKHTQCVFL FGHLPF*KRKHLK*SLGHNRLGQT*HLVALQPF SFVCFPFISSPDLNNWRNFLQFFFPSLAGIPSS QLTISQYKTPK*PIYNLPSQVFLLFLDECTMRNS TLIILDFLITKKKMKDLKAPEQFIDQFESIYLH LNVF*NM*KVIKCIFHATCTIRTSIIVYMPLMY FPLKIINCVFDSEYSIWYQLKFLVINVIRIVSQT ENM*SSIKIWPNKCSVSTLINGYSFLF

Sequence RSN.aAug05

GGCGGCCGCGGCGGCTGCTGCTGCTGCAGTGGGACAGGTGGCGGCGACCGGCGGCGTCCGAGGAGATTTAATCCAGAGACTGACTTCACTATAGAACCCA CAGTTGTATCAATGGTTGGGGAAAGATAGTGGCAACAGGCAAAGGAGAAACAGCTCTGACATACAAAGAAAATGAGTATGCTAAAGCCAAGTGGGCTTAA GGCCCCCACCAAGATCCTGAAGCCTGGAAGCACAGCTCTGAAGACACCTACGGCTGTTGTAGCTCCAGTAGAAAAAACCATATCCAGTGAAAAAGCATCA AGCACTCCATCATCTGAGACTCAGGAGGAATTTGTGGATGACTTTCGAGTTGGGGAGCGAGTTTGGGTGAATGGAAATAAGCCTGGATTTATCCAGTTTC TTGGAGAAACCCAGTTTGCACCAGGCCAGTGGGCTGGAATTGTTTTAGATGAACCCATAGGCAAGAACGATGGTTCGGTGGCAGGAGTTCGGTATTTCCA GTGTGAACCTTTAAAGGGCATATTTACCCGACCTTCAAAGTTAACAAGGAAGGTGCAAGCAGAAGATGAAGCTAATGGCCTGCAGACAACGCCCGCCTCC CGAGCTACTTCACCGCTGTGCACTTCTACGGCCAGCATGGTGTCTTCCTCCCCCTCCACCCCTTCAAACATCCCTCAGAAACCATCACAGCCAGCAGCAA AGGAACCTTCAGCTACGCCTCCGATCAGCAACCTTACAAAAACTGCCAGTGAATCTATCTCCAACCTTTCAGAGGCTGGCTCAATCAAGAAAGGAGAAAG AGAGCTCAAAATCGGAGACAGAGTATTGGTTGGTGGCACTAAGGCTGGTGTAGTCCGGTTTCTTGGGGAGACCGACTTTGCCAAGGGGGAGTGGTGTGGC GTGGAGTTAGATGAGCCACTTGGGAAGAATGATGGCGCTGTTGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACA AAGTTACCAAGATTGGCTTCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGTCCGCCAGCCTGAAGCGCAG CCCTTCTGCCTCTTCCCTCAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAGCAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTAC GCCAGGAAGATCTCCGGTACCACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGGAGAGGGCGG AGGTGGCCAAGGCCACGAGCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGACGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAAT GGACCAGCTGCGAACAATGGTGGAAGCTGCTGACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTC CGGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGACGCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTTGAACAGAGCCTGCTCTTTGAAA AGACCAAAGCTGACAAACTCCAGAGGGAGTTAGAAGACACTAGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCATT GAGAGTACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAATAAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCT TTGCAAGAAAAGTTAGAAGTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTCATCAGAAGGAGA TAAAGGCTCTGTATACCGCCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGC TCTATGGAAGTCCAAACTGGAGACTGCCATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCA GAATTTGCTGAACTAAAAACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTTGCAGAATCAACAAGACTCTGAACGGGCTGCCC ATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGATGAAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGA AGACCAGCATCTCGTAGAAATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGGTAAAGGAGCTAGAGGTACTGCAAGCCAAATGCAATGAA CAAACCAAGGTTATTGATAATTTTACATCACAGCTCAAGGCTACTGAAGAAAAGCTCTTGGATCTTGATGCACTTCGGAAAGCCAGTTCCGAAGGTAAAT CGGAAATGAAGAAACTTAGACAGCAGCTTGAGGCAGCTGAGAAACAGATTAAACATTTAGAGATTGAAAAGAATGCTGAAAGTAGCAAGGCTAGTAGCAT TACCAGAGAGCTCCAGGGGAGAGAGCTAAAGCTTACTAACCTTCAGGAAAATTTGAGTGAAGTCAGTCAAGTGAAAGAGACTTTGGAAAAAGAACTTCAG ATTTTGAAAGAAAAGTTTGCTGAAGCTTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAAGAAACTGTAAATAAGTTACACCAAAAGGAGGAACAGT TTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAATTTAGAGAGAAAGATGAGAGAGAAGAGCAGCTGATAAA GGCAAAGGAAAAACTGGAAAATGACATTGCAGAAATAATGAAGATGTCAGGAGATAACTCTTCTCAGCTGACAAAAATGAACGATGAATTACGTCTGAAA GAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTGCAAAAAAGTATTGAGGACATGACTGTCAAAGCTGAAC AGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGAATTGGAGAGGAAATTGTCGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCA GTGTCAGGAGCTGAAAGCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGAAATCCTACAGAACCTCCAGAAGACGCTGCTGGACACA GAGGACAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGGAGCTGAGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAG CGGAAGATGCCATGCAGATAATGGAACAGATGACCAAAGAGAAGACTGAGACTCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAAACTACAGAA TGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGAACTCCTGACTGTAGAGAATCAAAAAATGGAAGAATTT AGGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCAGCGTTGCAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGA GAAGCAGGGACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATCAGTTGTTAGAAATGAAAAAAAGAGAATCCAAGTTCAT AAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTATAACTAGTGCCTTACTCACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAAT GAGGTCACAGTGCTCAGGGGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACTCTAGAGTCTGATAAGGTGAAGCTCGAGCTCAAGGTAA AGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAATACAGACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAG TCAGATTGATTTCCTAAATTCAGTAATAGTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATGGG AACGGGGATGACCTAAACAATTATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCTCTTCTGTGACATTTGTGACTGCTTTGATCTCC ACGACACAGAGGATTGTCCTACCCAGGCACAGATGTCAGAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAAT CTGTGAGATGTTTGGACACTGGGCCACCAACTGCAATGACGACGAAACCTTCTGATGAAGCCTCCAGTGGAGAACTGGGCTTGCTCAGACGCACTCGCAT TGACACAACGTAACACCAGCATTGTGTGTGCAGACTTCAGGAGAACTCATGTTATTTTTTAACCCCGTCAACAAATCTAGGAAAATATTTTGATCTTCAA CAAATTGCCCTTTAGTCTCCCCGTATGAGTTAGAATAATAAATATTTAGTAGGTGAGTTTTCACCTCGAATTTTGTTTTCTTGATTTTTACGTTTGAAGA CATTGCACCAGATGCCATTACATTTATTGGCCCCCCGACCTTGTAGAAAAACCCCTACCCTCACAATACCTTATTTAAGTAACTTTAAATTATGCCGTTA CTTTTCATATTTGCACTAAGATATTTCCAGGCTGCATTTGTATATTTAGATTTTTTGGTTAAGCTTTGACACTGGAATGAGTTGAAAAAATGTGCCATTT TGCATTTTCATCTACTCATTTAAAGTATTTTATTCTTATTCAAAGAAATATCTGAGCTCTTTGCACTACCTGTTATCAGTAGTGCCTTTACTTCAGGCTT GATAATACTTAGGTGTGATTATAAAATCATGAAGCAGGTAAAGGGAGGGGCAAGCCCCCAAACTGCTGTGGGGACATTTTATAATCTATATGCTGCACCC ACTTAATCTACTGTGGTGTTTTGTTTATTAGTTTTGCATAATTTCAGCTTCTATATATTGTATGTATATATTTTTTAAAAATCTATATTTTGGGAAAAAA ACATACACAATGTGTCTTTCTTTTTGGACATTTACCTTTTTGAAAAAGAAAACACTTAAAATGATCATTAGGACATAACAGACTAGGCCAGACATAGCAT CTTGTGGCTTTGCAACCATTTTCATTTGTTTGTTTTCCTTTTATTTCTTCACCAGATTTAAATAACTGGAGGAATTTTCTCCAATTTTTTTTTCCTTCTC TGGCAGGTATCCCCAGCAGTCAATTAACAATAAGCCAGTATAAAACACCTAAATAACCAATCTACAATCTCCCTTCACAAGTTTTTTTACTGTTTTTAGA TGAATGTACGATGAGAAATTCAACGTTAATAATTCTGGATTTTCTTATCACAAAAAAGAAAATGAAGGACCTCAAAGCACCTGAACAGTTTATCGACCAG TTTGAATCTATTTATCTTCATTTGAATGTCTTCTAGAATATGTAAAAAGTCATAAAATGTATCTTCCATGCTACATGTACAATAAGAACTTCTATAATTG TATATATGCCTTTGATGTATTTTCCCCTCAAGATTATCAACTGTGTGTTCGACAGTGAATATTCAATCTGGTACCAGTTGAAATTTTTGGTTATAAATGT AATACGAATTGTTTCACAAACAGAAAACATGTAAAGCAGTATTAAAATTTGGCCAAACAAGTGTTCTGTATCTACTTTAATAAATGGTTATTCTTTTTTG TTT

RSN.F35 (forward primer)
RSN.R13 (reverse primer)
Single exon skipping

Frame 0

GGRGGCCCCSGTGGGDRRRPRRFNPETDFTIEP TVVSMVGER*WQQAKEKQL*HTKKMSMLKPSGLK APTKILKPGSTALKTPTAVVAPVEKTISSEKAS STPSSETQEEFVDDFRVGERVWVNGNKPGFIQF LGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQ CEPLKGIFTRPSKLTRKVQAEDEANGLQTTPAS RATSPLCTSTASMVSSSPSTPSNIPQKPSQPAA KEPSATPPISNLTKTASESISNLSEAGSIKKGER ELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCG VELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVH KVTKIGFPSTTPAKAKANAVRRVMATTSASLKRS PSASSLSSMSSVASSVSSRPSRTGLLTETSSRY ARKISGTTALQEALKEKQQHIEQLLAERDLERA EVAKATSHVGEIEQELALARDGHDQHVLELEAKM DQLRTMVEAADREKVELLNQLEEEKRKVEDLQF RVEEESITKGDLETQTKLEHARIKELEQSLLFE KTKADKLQRELEDTRVATVSEKSRIMELEKDLAL RVQEVAELRRRLESNKPAGDVDMSLSLLQEISS LQEKLEVTRTDHQREITSLKEHFGAREETHQKE IKALYTATEKLSKENESLKSKLEHANKENSDVIA LWKSKLETAIASHQQAMEELKVSFSKGLGTETA EFAELKTQIEKMRLDYQHEIENLQNQQDSERAA HAKEMEALRAKLMKVIKEKENSLEAIRSKLDKAE DQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNE QTKVIDNFTSQLKATEEKLLDLDALRKASSEGK SEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSI TRELQGRELKLTNLQENLSEVSQVKETLEKELQ ILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQ FNMLSSDLEKLRENLADMEAKFREKDEREEQLIK AKEKLENDIAEIMKMSGDNSSQLTKMNDELRLK ERDVEELQLKLTKANENASFLQKSIEDMTVKAE QSQQEAAKKHEEEKKELERKLSDLEKKMETSHNQ CQELKARYERATSETKTKHEEILQNLQKTLLDT EDKLKGAREENSGLLQELEELRKQADKAKAAQT AEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQN ELDTLKENNLKNVEELNKSKELLTVENQKMEEF RKEIETLKQAAAQKSQQLSALQEENVKLAEELG RSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFI KDADEEKASLQKSISITSALLTEKDAELEKLRN EVTVLRGENASAKSLHSVVQTLESDKVKLELKV KNLELQLKENKRQLSSSSGNTDTQADEDERAQES QIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNG NGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDL HDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEI CEMFGHWATNCNDDETF**SLQWRTGLAQTHSH *HNVTPALCVQTSGELMLFFNPVNKSRKIF*SS TNCPLVSPYELE**IFSR*VFTSNFVFLIFTFED IAPDAITFIGPPTL*KNPYPHNTLFK*L*IMPL LFIFALRYFQAAFVYLDFLVKL*HWNELKKCAI LHFHLLI*SILFLFKEISELFALPVISSAFTSGL IILRCDYKIMKQVKGGASPQTAVGTFYNLYAAP T*STVVFCLLVLHNFSFYILYVYIF*KSIFWEK NIHNVSFFLDIYLFEKENT*NDH*DITD*ARHSI LWLCNHFHLFVFLLFLHQI*ITGGIFSNFFFLL WQVSPAVN*Q*ASIKHLNNQSTISLHKFFYCF* MNVR*EIQR**FWIFLSQKRK*RTSKHLNSLSTS LNLFIFI*MSSRICKKS*NVSSMLHVQ*ELL*L YICL*CIFPSRLSTVCSTVNIQSGTS*NFWL*M *YELFHKQKTCKAVLKFGQTSVLYLL**MVILFC

Frame 1

AAAAAAAAAVGQVAATGGVRGDLIQRLTSL*NP QLYQWLGKDSGNRQRRNSSDIQRK*VC*SQVGL RPPPRS*SLEAQL*RHLRLL*LQ*KKPYPVKKHQ ALHHLRLRRNLWMTFELGSEFG*MEISLDLSSF LEKPSLHQASGLELF*MNP*ARTMVRWQEFGIS SVNL*RAYLPDLQS*QGRCKQKMKLMACRQRPPP ELLHRCALLRPAWCLPPPPPLQTSLRNHHSQQQ RNLQLRLRSATLQKLPVNLSPTFQRLAQSRKEK ESSKSETEYWLVALRLV*SGFLGRPTLPRGSGVA WS*MSHLGRMMALLLEQGIFSVNPNMACSLLST KLPRLASLPLHQPKPRPTQ*GE*WRPRPPA*SA ALLPLPSAP*AQWPPL*AAGPVGQDY*LKPPPVT PGRSPVPLPSRRP*RRSSSTLSSCWRNGIWRGR RWPRPRATWGR*SRS*LWPGTDMTSMSWNWKPK WTSCEQWWKLLTGRRWSFSTSLKRRKGRLRTFSS GLKKNQLPKVILRRRPNWSMPALRSLNRACSLK RPKLTNSRGS*KTLGWLQFQKSHV*WNWRKT*H *EYRK*LSSEEG*SPISLLGMWTCHFPFCKR*AL CKKS*KSPVLTTREK*LL*RSILEPGKKLIRRR *RLCIPPRKSFPKRTSH*KASWSMPTKRTQM** LYGSPNWRLPSHPTSRRWKN*RYLSAKGLEQRRQ NLLN*KHK*RK*D*ITNTK*KICRINKTLNGLP MLKRWKP*GLN**KLLKKRKTVWKPSGRNWTKQ KTSIS*KWKTR*TNYRKLK*R*RS*RYCKPNAMN KPRLLIILHHSSRLLKKSSWILMHFGKPVPKVN RK*RNLDSSLRQLRNRLNI*RLKRMLKVARLVA LPESSRGES*SLLTFRKI*VKSVK*KRLWKKNFR F*KKSLLKLQRRQSLFREVCKKL*ISYTKRRNS LTCCLLTWRS*EKT*QIWRQNLERKMREKSS** RQRKNWKMTLQK**RCQEITLLS*QK*TMNYV*K KEM*KNYS*NLQRLMKMQVFCKKVLRT*LSKLN RASKKQLKSMRKKRKNWRGNCRTWKRKWKQATT SVRS*KPGMREPLLRQKPSMKKSYRTSRRRCWTQ RTS*RAHGRRTVACCRSWRS*ESKPTKPKLLKQ RKMPCR*WNR*PKRRLRLWPPWRTPSKQMQNYR MNWTHLKKTT*KMWKS*TNQKNS*L*RIKKWKNL GKK*KP*SRQQLRSPSSFQRCKKRTLNLLRSWG EAGTKSQVIKSWKKKDLCSIISC*K*KKENPSS *KTQMKRKLPCRNPSV*LVPYSQKRMPSWRN*EM RSQCSGEKTPLPSPCIQLFRL*SLIR*SSSSR* RTWSFNSKKTRGSSAAPQVIQTLRQTRMKEPRR VRLIS*IQ**WTFKGRIKTSR*RWR*CQKQP*MG TGMT*TIMTVMIRRNSPRRNLASSVTFVTALIS TTQRIVLPRHRCQRTLPIPHTMAVGVRNAHTVK SVRCLDTGPPTAMTTKPSDEASSGELGLLRRTRI DTT*HQHCVCRLQENSCYFLTPSTNLGKYFDLQ QIAL*SPRMS*NNKYLVGEFSPRILFS*FLRLK TLHQMPLHLLAPRPCRKTPTLTIPYLSNFKLCRY FSYLH*DISRLHLYI*IFWLSFDTGMS*KNVPF CIFIYSFKVFYSYSKKYLSSLHYLLSVVPLLQA **YLGVIIKS*SR*REGQAPKLLWGHFIIYMLHP LNLLWCFVY*FCIISASIYCMYIFFKNLYFGKK TYTMCLSFWTFTFLKKKTLKMIIRT*QTRPDIA SCGFATIFICLFSFYFFTRFK*LEEFSPIFFSFS GRYPQQSINNKPV*NT*ITNLQSPFTSFFTVFR *MYDEKFNVNNSGFSYHKKENEGPQST*TVYRP V*IYLSSFECLLEYVKSHKMYLPCYMYNKNFYNC IYAFDVFSPQDYQLCVRQ*IFNLVPVEIFGYKC NTNCFTNRKHVKQY*NLAKQVFCIYFNKWLFFF V

Frame 2

RPRRLLLLQWDRWRRPAASEEI*SRD*LHYRTH SCINGWGKIVATGKGETALTYKENEYAKAKWA* GPHQDPEAWKHSSEDTYGCCSSSRKNHIQ*KSI KHSII*DSGGICG*LSSWGASLGEWK*AWIYPVS WRNPVCTRPVGWNCFR*THRQERWFGGRSSVFP V*TFKGHIYPTFKVNKEGASRR*S*WPADNARL PSYFTAVHFYGQHGVFLPLHPFKHPSETITASSK GTFSYASDQQPYKNCQ*IYLQPFRGWLNQERRK RAQNRRQSIGWWH*GWCSPVSWGDRLCQGGVVW RGVR*ATWEE*WRCCWNKVFSVSTQIWLVRSCPQ SYQDWLPFHYTSQSQGQRSEASDGDHVRQPEAQ PFCLFPQLHELSGLLCEQQAQSDRTID*NLLPL RQEDLRYHCPPGGPEGEAAAH*AAAGGTGSGEGG GGQGHEPRGGDRAGASSGPGRT*PACPGIGSQN GPAANNGGSC*QGEGGASQPA*RGEKEG*GPSV PG*RRINYQR*S*DADQTGACPH*GA*TEPAL*K DQS*QTPEGVRRH*GGYSFRKVTYNGTGERPSI ESTGSS*APKKARVQ*ACWGCGHVTFPFARDKL FARKVRSHPY*PPERNNFSEGAFWSPGRNSSEGD KGSVYRHGKAFQRERVIEKQAGACQQRELRCDS SMEVQTGDCHRIPPAGDGRTEGIFQQRAWNRDG RIC*TKNTNRENETRLPTRNRKFAESTRL*TGCP C*RDGSLEG*TDESY*RKGKQSGSHQVETGQSR RPASRRNGRHVKQITGS*NKGKGARGTASQMQ* TNQGY**FYITAQGY*RKALGS*CTSESQFRR*I GNEET*TAA*GS*ETD*TFRD*KEC*K*QG**H YQRAPGERAKAY*PSGKFE*SQSSERDFGKRTS DFERKVC*SFRGGSLCSEKYARNCK*VTPKGGTV *HAVF*LGEAERKLSRYGGKI*RER*ERRAADK GKGKTGK*HCRNNEDVRR*LFSADKNER*ITSE RKRCRRITAKTYKG**KCKFSAKKY*GHDCQS*T EPARSS*KA*GRKERIGEEIVGPGKENGNKPQP VSGAESQV*ESHF*DKNQA*RNPTEPPEDAAGH RGQAEGRTGGEQWLAAGAGGAEKASRQSQSCSNS GRCHADNGTDDQRED*DSGLLGGHQANKCKTTE *IGHT*RKQLEKCGRAEQIKRTPDCRESKNGRI *ERNRNPKAGSSSEVPAAFSVARRER*TC*GAGE KQGRSHKSSKAGRRKICAQ*SVVRNEKKRIQVH KRRR*RESFLAEIHQYN*CLTHRKGCRAGETEK *GHSAQGRKRLCQVLAFSCSDSRV**GEARAQGK ELGASTQRKQEAAQQLLR*YRHSGRRG*KSPGE SD*FPKFSNSGPSKEESRPQDEGGDDVRSSPEW ERG*PKQL*Q**SGETVQEETSPLL*HL*LL*SP RHRGLSYPGTDVRGPSPFHTPWQSG*GTPIL*N L*DVWTLGHQLQ*RRNLLMKPPVENWACSDALA LTQRNTSIVCADFRRTHVIF*PRQQI*ENILIFN KLPFSLPV*VRIINI**VSFHLEFCFLDFYV*R HCTRCHYIYWPPDLVEKPLPSQYLI*VTLNYAV TFHICTKIFPGCICIFRFFG*ALTLE*VEKMCHF AFSSTHLKYFILIQRNI*ALCTTCYQ*CLYFRL DNT*V*L*NHEAGKGRGKPPNCCGDIL*SICCT HLIYCGVLFISFA*FQLLYIVCIYFLKIYILGKK HTQCVFLFGHLPF*KRKHLK*SLGHNRLGQT*H LVALQPFSFVCFPFISSPDLNNWRNFLQFFFPS LAGIPSSQLTISQYKTPK*PIYNLPSQVFLLFLD ECTMRNSTLIILDFLITKKKMKDLKAPEQFIDQ FESIYLHLNVF*NM*KVIKCIFHATCTIRTSII VYMPLMYFPLKIINCVFDSEYSIWYQLKFLVINV IRIVSQTENM*SSIKIWPNKCSVSTLINGYSFL F

Sequence RSN.kAug05

CATGTCCTGGAATTGGAAGCCAAAATGGACCAGCTGCGAACAATGGTGGAAGCTGCTGACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGA AAAGGAAGGTTGAGGACCTTCAGTTCCGGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGCAAAAGAGCCAGATTTCTGAAGATCCTGAGAATAC GCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTTGAACAGAGCCTGCTCTTTGAAAAGACCAAAGCTGACAAACTCCAGAGGGAGTTAGAAGACACT AGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCATTGAGAGTACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGT CCAATAAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCAAGAAAAGTTAGAAGTCACCCGTACTGACCACCAGAG AGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTCATCAGAAGGAGATAAAGGCTCTGTATACCGCCACGGAAAAGCTTTCCAAAGAG AACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGCTCTATGGAAGTCCAAACTGGAGACTGCCATCGCATCCCACC AGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCAGAATTTGCTGAACTAAAAACACAAATAGAGAAAATGAGACT AGATTACCAACACGAAATAGAAAATTTGCAGAATCAACAAGACTCTGAACGGGCTGCCCATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGATGAAA GTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGT

RSN.F35 (forward primer)
RSN.R13 (reverse primer)
Single exon skipping

Frame 0

HVLELEAKMDQLRTMVEAADREKVELLNQLEEE KRKVEDLQFRVEEESITKGDLEQKSQISEDPENT QTKLEHARIKELEQSLLFEKTKADKLQRELEDT RVATVSEKSRIMELEKDLALRVQEVAELRRRLE SNKPAGDVDMSLSLLQEISSLQEKLEVTRTDHQR EITSLKEHFGAREETHQKEIKALYTATEKLSKE NESLKSKLEHANKENSDVIALWKSKLETAIASH QQAMEELKVSFSKGLGTETAEFAELKTQIEKMRL DYQHEIENLQNQQDSERAAHAKEMEALRAKLMK VIKEKENSLEAIR

Frame 1

MSWNWKPKWTSCEQWWKLLTGRRWSFSTSLKRR KGRLRTFSSGLKKNQLPKVILSKRARFLKILRI RRPNWSMPALRSLNRACSLKRPKLTNSRGS*KTL GWLQFQKSHV*WNWRKT*H*EYRK*LSSEEG*S PISLLGMWTCHFPFCKR*ALCKKS*KSPVLTTR EK*LL*RSILEPGKKLIRRR*RLCIPPRKSFPKR TSH*KASWSMPTKRTQM**LYGSPNWRLPSHPT SRRWKN*RYLSAKGLEQRRQNLLN*KHK*RK*D *ITNTK*KICRINKTLNGLPMLKRWKP*GLN**K LLKKRKTVWKPSG

Frame 2

CPGIGSQNGPAANNGGSC*QGEGGASQPA*RGE KEG*GPSVPG*RRINYQR*S*AKEPDF*RS*EY ADQTGACPH*GA*TEPAL*KDQS*QTPEGVRRH *GGYSFRKVTYNGTGERPSIESTGSS*APKKARV Q*ACWGCGHVTFPFARDKLFARKVRSHPY*PPE RNNFSEGAFWSPGRNSSEGDKGSVYRHGKAFQR ERVIEKQAGACQQRELRCDSSMEVQTGDCHRIPP AGDGRTEGIFQQRAWNRDGRIC*TKNTNRENET RLPTRNRKFAESTRL*TGCPC*RDGSLEG*TDE SY*RKGKQSGSHQ

Sequence RSN.cAug05

Frame 0

Frame 1

Frame 2

Sequence RSN.aAug05

Frame 0

Frame 1

Frame 2

Sequence RSN.kAug05

Frame 0

Frame 1

Frame 2

Sequence RSN.kAug05.extended

Frame 0

Frame 1

Frame 2