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Sequences

Sequence RSN.cAug05

CTTGGGAAGAATGATGGCGCTGTTGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACAAAGTTACCAAGATTGGCT
TCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGTCCGCCAGCCTGAAGCGCAGCCCTTCTGCCTCTTCCCT
CAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAGCAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTACGCCAGGAAGATCTCCGGT
ACCACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGGAGAGGGCGGAGGTGGCCAAGGCCACGA
GCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGACGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAATGGACCAGCTGCGAACAAT
GGTGGAAGCTGCTGACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTCCGGGTTGAAGAAGAATCA
ATTACCAAAGGTGATCTTGAGACGCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTTGAACAGAGCCTGCTCTTTGAAAAGACCAAAGCTGACAAAC
TCCAGAGGGAGTTAGAAGACACTAGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCATTGAGAGTACAGGAAGTAGC
TGAGCTCCGAAGAAGGCTAGAGTCCAATAAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCAAGAAAAGTTAGAA
GTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTCATCAGAAGGAGATAAAGGCTCTGTATACCG
CCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGCTCTATGGAAGTCCAAACT
GGAGACTGCCATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCAGAATTTGCTGAACTAAAA
ACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTTGCAGAATCAACAAGACTCTGAACGGGCTGCCCATGCTAAAGAGATGGAAG
CCTTGAGGGCTAAACTGATGAAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGAAGACCAGCATCTCGTAGA
AATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGAAAGAAAAGTTTGCTGAAGCTTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAA
GAAACTGTAAATAAGTTACACCAAAAGGAGGAACAGTTTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAAT
TTAGAGAGAAAGATGAGAGAGAAGAGCAGCTGATAAAGGCAAAGGAAAAACTGGAAAATGACATTGCAGAAATAATGAAGATGTCAGGAGATAACTCTTC
TCAGCTGACAAAAATGAACGATGAATTACGTCTGAAAGAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTG
CAAAAAAGTATTGAGGACATGACTGTCAAAGCTGAACAGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGAATTGGAGAGGAAATTGT
CGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCAGTGTCAGGAGCTGAAAGCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGA
AATCCTACAGAACCTCCAGAAGACGCTGCTGGACACAGAGGACAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGGAGCTG
AGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAGCGGAAGATGCCATGCAGATAATGGAACAGATGACCAAAGAGAAGACTGAGACTCTGGCCTCCT
TGGAGGACACCAAGCAAACAAATGCAAAACTACAGAATGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGA
ACTCCTGACTGTAGAGAATCAAAAAATGGAAGAATTTAGGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCAGCGTTG
CAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGAGAAGCAGGGACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATC
AGTTGTTAGAAATGAAAAAAAGAGAATCCAAGTTCATAAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTATAACTAGTGCCTTACT
CACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAATGAGGTCACAGTGCTCAGGGGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACT
CTAGAGTCTGATAAGGTGAAGCTCGAGCTCAAGGTAAAGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAATACAG
ACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAGTCAGTTCATCTGCTGCTGACAACACTTTTCTTCCAATCAGCAAATGATTGATTTCCTAAATTC
AGTAATAGTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATGGGAACGGGGATGACCTAAACAAT
TATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCTCTTCTGTGACATTTGTGACTGCTTTGATCTCCACGACACAGAGGATTGTCCTA
CCCAGGCACAGATGTCAGAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAATCTGTGAGATGTTTGGACACTG
GGCCACCAACTGCAATGACGACGAAACCTTCTGATGAAGCCTCCAGTGGAGAACTGGGCTTGCTCAGACGCACTCGCATTGACACAACGTAACACCAGCA
TTGTGTGTGCAGACTTCAGGAGAACTCATGTTATTTTTTAACCCCGTCAACAAATCTAGGAAAATATTTTGATCTTCAACAAATTGCCCTTTAGTCTCCC
CGTATGAGTTAGAATAATAAATATTTAGTAGGTGAGTTTTCACCTCGAATTTTGTTTTCTTGATTTTTACGTTTGAAGACATTGCACCAGATGCCATTAC
ATTTATTGGCCCCCCGACCTTGTAGAAAAACCCCTACCCTCACAATACCTTATTTAAGTAACTTTAAATTATGCCGTTACTTTTCATATTTGCACTAAGA
TATTTCCAGGCTGCATTTGTATATTTAGATTTTTTGGTTAAGCTTTGACACTGGAATGAGTTGAAAAAATGTGCCATTTTGCATTTTCATCTACTCATTT
AAAGTATTTTATTCTTATTCAAAGAAATATCTGAGCTCTTTGCACTACCTGTTATCAGTAGTGCCTTTACTTCAGGCTTGATAATACTTAGGTGTGATTA
TAAAATCATGAAGCAGGTAAAGGGAGGGGCAAGCCCCCAAACTGCTGTGGGGACATTTTATAATCTATATGCTGCACCCACTTAATCTACTGTGGTGTTT
TGTTTATTAGTTTTGCATAATTTCAGCTTCTATATATTGTATGTATATATTTTTTAAAAATCTATATTTTGGGAAAAAAACATACACAATGTGTCTTTCT
TTTTGGACATTTACCTTTTTGAAAAAGAAAACACTTAAAATGATCATTAGGACATAACAGACTAGGCCAGACATAGCATCTTGTGGCTTTGCAACCATTT
TCATTTGTTTGTTTTCCTTTTATTTCTTCACCAGATTTAAATAACTGGAGGAATTTTCTCCAATTTTTTTTTCCTTCTCTGGCAGGTATCCCCAGCAGTC
AATTAACAATAAGCCAGTATAAAACACCTAAATAACCAATCTACAATCTCCCTTCACAAGTTTTTTTACTGTTTTTAGATGAATGTACGATGAGAAATTC
AACGTTAATAATTCTGGATTTTCTTATCACAAAAAAGAAAATGAAGGACCTCAAAGCACCTGAACAGTTTATCGACCAGTTTGAATCTATTTATCTTCAT
TTGAATGTCTTCTAGAATATGTAAAAAGTCATAAAATGTATCTTCCATGCTACATGTACAATAAGAACTTCTATAATTGTATATATGCCTTTGATGTATT
TTCCCCTCAAGATTATCAACTGTGTGTTCGACAGTGAATATTCAATCTGGTACCAGTTGAAATTTTTGGTTATAAATGTAATACGAATTGTTTCACAAAC
AGAAAACATGTAAAGCAGTATTAAAATTTGGCCAAACAAGTGTTCTGTATCTACTTTAATAAATGGTTATTCTTTTTTGTTT
  • RSN.F10 (forward primer)
  • RSN.R9 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

LGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIG
FPSTTPAKAKANAVRRVMATTSASLKRSPSASSL
SSMSSVASSVSSRPSRTGLLTETSSRYARKISG
TTALQEALKEKQQHIEQLLAERDLERAEVAKAT
SHVGEIEQELALARDGHDQHVLELEAKMDQLRTM
VEAADREKVELLNQLEEEKRKVEDLQFRVEEES
ITKGDLETQTKLEHARIKELEQSLLFEKTKADK
LQRELEDTRVATVSEKSRIMELEKDLALRVQEVA
ELRRRLESNKPAGDVDMSLSLLQEISSLQEKLE
VTRTDHQREITSLKEHFGAREETHQKEIKALYT
ATEKLSKENESLKSKLEHANKENSDVIALWKSKL
ETAIASHQQAMEELKVSFSKGLGTETAEFAELK
TQIEKMRLDYQHEIENLQNQQDSERAAHAKEME
ALRAKLMKVIKEKENSLEAIRSKLDKAEDQHLVE
MEDTLNKLQEAEIKKEKFAEASEEAVSVQRSMQ
ETVNKLHQKEEQFNMLSSDLEKLRENLADMEAK
FREKDEREEQLIKAKEKLENDIAEIMKMSGDNSS
QLTKMNDELRLKERDVEELQLKLTKANENASFL
QKSIEDMTVKAEQSQQEAAKKHEEEKKELERKL
SDLEKKMETSHNQCQELKARYERATSETKTKHEE
ILQNLQKTLLDTEDKLKGAREENSGLLQELEEL
RKQADKAKAAQTAEDAMQIMEQMTKEKTETLAS
LEDTKQTNAKLQNELDTLKENNLKNVEELNKSKE
LLTVENQKMEEFRKEIETLKQAAAQKSQQLSAL
QEENVKLAEELGRSRDEVTSHQKLEEERSVLNN
QLLEMKKRESKFIKDADEEKASLQKSISITSALL
TEKDAELEKLRNEVTVLRGENASAKSLHSVVQT
LESDKVKLELKVKNLELQLKENKRQLSSSSGNT
DTQADEDERAQESQFICC*QHFSSNQQMIDFLNS
VIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNN
YDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCP
TQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHW
ATNCNDDETF**SLQWRTGLAQTHSH*HNVTPA
LCVQTSGELMLFFNPVNKSRKIF*SSTNCPLVS
PYELE**IFSR*VFTSNFVFLIFTFEDIAPDAIT
FIGPPTL*KNPYPHNTLFK*L*IMPLLFIFALR
YFQAAFVYLDFLVKL*HWNELKKCAILHFHLLI
*SILFLFKEISELFALPVISSAFTSGLIILRCDY
KIMKQVKGGASPQTAVGTFYNLYAAPT*STVVF
CLLVLHNFSFYILYVYIF*KSIFWEKNIHNVSF
FLDIYLFEKENT*NDH*DITD*ARHSILWLCNHF
HLFVFLLFLHQI*ITGGIFSNFFFLLWQVSPAV
N*Q*ASIKHLNNQSTISLHKFFYCF*MNVR*EI
QR**FWIFLSQKRK*RTSKHLNSLSTSLNLFIFI
*MSSRICKKS*NVSSMLHVQ*ELL*LYICL*CI
FPSRLSTVCSTVNIQSGTS*NFWL*M*YELFHK
QKTCKAVLKFGQTSVLYLL**MVILFC

Frame 1

LGRMMALLLEQGIFSVNPNMACSLLSTKLPRLA
SLPLHQPKPRPTQ*GE*WRPRPPA*SAALLPLP
SAP*AQWPPL*AAGPVGQDY*LKPPPVTPGRSPV
PLPSRRP*RRSSSTLSSCWRNGIWRGRRWPRPR
ATWGR*SRS*LWPGTDMTSMSWNWKPKWTSCEQ
WWKLLTGRRWSFSTSLKRRKGRLRTFSSGLKKNQ
LPKVILRRRPNWSMPALRSLNRACSLKRPKLTN
SRGS*KTLGWLQFQKSHV*WNWRKT*H*EYRK*
LSSEEG*SPISLLGMWTCHFPFCKR*ALCKKS*K
SPVLTTREK*LL*RSILEPGKKLIRRR*RLCIP
PRKSFPKRTSH*KASWSMPTKRTQM**LYGSPN
WRLPSHPTSRRWKN*RYLSAKGLEQRRQNLLN*K
HK*RK*D*ITNTK*KICRINKTLNGLPMLKRWK
P*GLN**KLLKKRKTVWKPSGRNWTKQKTSIS*
KWKTR*TNYRKLK*RKKSLLKLQRRQSLFREVCK
KL*ISYTKRRNSLTCCLLTWRS*EKT*QIWRQN
LERKMREKSS**RQRKNWKMTLQK**RCQEITL
LS*QK*TMNYV*KKEM*KNYS*NLQRLMKMQVFC
KKVLRT*LSKLNRASKKQLKSMRKKRKNWRGNC
RTWKRKWKQATTSVRS*KPGMREPLLRQKPSMK
KSYRTSRRRCWTQRTS*RAHGRRTVACCRSWRS*
ESKPTKPKLLKQRKMPCR*WNR*PKRRLRLWPP
WRTPSKQMQNYRMNWTHLKKTT*KMWKS*TNQK
NS*L*RIKKWKNLGKK*KP*SRQQLRSPSSFQRC
KKRTLNLLRSWGEAGTKSQVIKSWKKKDLCSII
SC*K*KKENPSS*KTQMKRKLPCRNPSV*LVPY
SQKRMPSWRN*EMRSQCSGEKTPLPSPCIQLFRL
*SLIR*SSSSR*RTWSFNSKKTRGSSAAPQVIQ
TLRQTRMKEPRRVSSSAADNTFLPISK*LIS*I
Q**WTFKGRIKTSR*RWR*CQKQP*MGTGMT*TI
MTVMIRRNSPRRNLASSVTFVTALISTTQRIVL
PRHRCQRTLPIPHTMAVGVRNAHTVKSVRCLDT
GPPTAMTTKPSDEASSGELGLLRRTRIDTT*HQH
CVCRLQENSCYFLTPSTNLGKYFDLQQIAL*SP
RMS*NNKYLVGEFSPRILFS*FLRLKTLHQMPL
HLLAPRPCRKTPTLTIPYLSNFKLCRYFSYLH*D
ISRLHLYI*IFWLSFDTGMS*KNVPFCIFIYSF
KVFYSYSKKYLSSLHYLLSVVPLLQA**YLGVI
IKS*SR*REGQAPKLLWGHFIIYMLHPLNLLWCF
VY*FCIISASIYCMYIFFKNLYFGKKTYTMCLS
FWTFTFLKKKTLKMIIRT*QTRPDIASCGFATI
FICLFSFYFFTRFK*LEEFSPIFFSFSGRYPQQS
INNKPV*NT*ITNLQSPFTSFFTVFR*MYDEKF
NVNNSGFSYHKKENEGPQST*TVYRPV*IYLSS
FECLLEYVKSHKMYLPCYMYNKNFYNCIYAFDVF
SPQDYQLCVRQ*IFNLVPVEIFGYKCNTNCFTN
RKHVKQY*NLAKQVFCIYFNKWLFFFV

Frame 2

WEE*WRCCWNKVFSVSTQIWLVRSCPQSYQDWL
PFHYTSQSQGQRSEASDGDHVRQPEAQPFCLFP
QLHELSGLLCEQQAQSDRTID*NLLPLRQEDLR
YHCPPGGPEGEAAAH*AAAGGTGSGEGGGGQGHE
PRGGDRAGASSGPGRT*PACPGIGSQNGPAANN
GGSC*QGEGGASQPA*RGEKEG*GPSVPG*RRI
NYQR*S*DADQTGACPH*GA*TEPAL*KDQS*QT
PEGVRRH*GGYSFRKVTYNGTGERPSIESTGSS
*APKKARVQ*ACWGCGHVTFPFARDKLFARKVR
SHPY*PPERNNFSEGAFWSPGRNSSEGDKGSVYR
HGKAFQRERVIEKQAGACQQRELRCDSSMEVQT
GDCHRIPPAGDGRTEGIFQQRAWNRDGRIC*TK
NTNRENETRLPTRNRKFAESTRL*TGCPC*RDGS
LEG*TDESY*RKGKQSGSHQVETGQSRRPASRR
NGRHVKQITGS*NKERKVC*SFRGGSLCSEKYA
RNCK*VTPKGGTV*HAVF*LGEAERKLSRYGGKI
*RER*ERRAADKGKGKTGK*HCRNNEDVRR*LF
SADKNER*ITSERKRCRRITAKTYKG**KCKFS
AKKY*GHDCQS*TEPARSS*KA*GRKERIGEEIV
GPGKENGNKPQPVSGAESQV*ESHF*DKNQA*R
NPTEPPEDAAGHRGQAEGRTGGEQWLAAGAGGA
EKASRQSQSCSNSGRCHADNGTDDQRED*DSGLL
GGHQANKCKTTE*IGHT*RKQLEKCGRAEQIKR
TPDCRESKNGRI*ERNRNPKAGSSSEVPAAFSV
ARRER*TC*GAGEKQGRSHKSSKAGRRKICAQ*S
VVRNEKKRIQVHKRRR*RESFLAEIHQYN*CLT
HRKGCRAGETEK*GHSAQGRKRLCQVLAFSCSD
SRV**GEARAQGKELGASTQRKQEAAQQLLR*YR
HSGRRG*KSPGESVHLLLTTLFFQSAND*FPKF
SNSGPSKEESRPQDEGGDDVRSSPEWERG*PKQ
L*Q**SGETVQEETSPLL*HL*LL*SPRHRGLSY
PGTDVRGPSPFHTPWQSG*GTPIL*NL*DVWTL
GHQLQ*RRNLLMKPPVENWACSDALALTQRNTS
IVCADFRRTHVIF*PRQQI*ENILIFNKLPFSLP
V*VRIINI**VSFHLEFCFLDFYV*RHCTRCHY
IYWPPDLVEKPLPSQYLI*VTLNYAVTFHICTK
IFPGCICIFRFFG*ALTLE*VEKMCHFAFSSTHL
KYFILIQRNI*ALCTTCYQ*CLYFRLDNT*V*L
*NHEAGKGRGKPPNCCGDIL*SICCTHLIYCGV
LFISFA*FQLLYIVCIYFLKIYILGKKHTQCVFL
FGHLPF*KRKHLK*SLGHNRLGQT*HLVALQPF
SFVCFPFISSPDLNNWRNFLQFFFPSLAGIPSS
QLTISQYKTPK*PIYNLPSQVFLLFLDECTMRNS
TLIILDFLITKKKMKDLKAPEQFIDQFESIYLH
LNVF*NM*KVIKCIFHATCTIRTSIIVYMPLMY
FPLKIINCVFDSEYSIWYQLKFLVINVIRIVSQT
ENM*SSIKIWPNKCSVSTLINGYSFLF

Sequence RSN.bAug05

GGCGGCCGCGGCGGCTGCTGCTGCTGCAGTGGGACAGGTGGCGGCGACCGGCGGCGTCCGAGGAGATTTAATCCAGAGACTGACTTCACTATAGAACCCA
CAGTTGTATCAATGGTTGGGGAAAGATAGTGGCAACAGGCAAAGGAGAAACAGCTCTGACATACAAAGAAAATGAGTATGCTAAAGCCAAGTGGGCTTAA
GGCCCCCACCAAGATCCTGAAGCCTGGAAGCACAGCTCTGAAGACACCTACGGCTGTTGTAGCTCCAGTAGAAAAAACCATATCCAGTGAAAAAGCATCA
AGCACTCCATCATCTGAGACTCAGGAGGAATTTGTGGATGACTTTCGAGTTGGGGAGCGAGTTTGGGTGAATGGAAATAAGCCTGGATTTATCCAGTTTC
TTGGAGAAACCCAGTTTGCACCAGGCCAGTGGGCTGGAATTGTTTTAGATGAACCCATAGGCAAGAACGATGGTTCGGTGGCAGGAGTTCGGTATTTCCA
GTGTGAACCTTTAAAGGGCATATTTACCCGACCTTCAAAGTTAACAAGGAAGGTGCAAGCAGAAGATGAAGCTAATGGCCTGCAGACAACGCCCGCCTCC
CGAGCTACTTCACCGCTGTGCACTTCTACGGCCAGCATGGTGTCTTCCTCCCCCTCCACCCCTTCAAACATCCCTCAGAAACCATCACAGCCAGCAGCAA
AGGAACCTTCAGCTACGCCTCCGATCAGCAACCTTACAAAAACTGCCAGTGAATCTATCTCCAACCTTTCAGAGGCTGGCTCAATCAAGAAAGGAGAAAG
AGAGCTCAAAATCGGAGACAGAGTATTGGTTGGTGGCACTAAGGCTGGTGTAGTCCGGTTTCTTGGGGAGACCGACTTTGCCAAGGGGGAGTGGTGTGGC
GTGGAGTTAGATGAGCCACTTGGGAAGAATGATGGCGCTGTTGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACA
AAGTTACCAAGATTGGCTTCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGTCCGCCAGCCTGAAGCGCAG
CCCTTCTGCCTCTTCCCTCAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAGCAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTAC
GCCAGGAAGATCTCCGGTACCACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGGAGAGGGCGG
AGGTGGCCAAGGCCACGAGCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGACGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAAT
GGACCAGCTGCGAACAATGGTGGAAGCTGCTGACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTC
CGGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCATTGAGAG
TACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAATAAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCA
AGAAAAGTTAGAAGTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTCATCAGAAGGAGATAAAG
GCTCTGTATACCGCCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGCTCTAT
GGAAGTCCAAACTGGAGACTGCCATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCAGAATT
TGCTGAACTAAAAACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTTGCAGAATCAACAAGACTCTGAACGGGCTGCCCATGCT
AAAGAGATGGAAGCCTTGAGGGCTAAACTGATGAAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGAAGACC
AGCATCTCGTAGAAATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGGTAAAGGAGCTAGAGGTACTGCAAGCCAAATGCAATGAACAAAC
CAAGGTTATTGATAATTTTACATCACAGCTCAAGGCTACTGAAGAAAAGCTCTTGGATCTTGATGCACTTCGGAAAGCCAGTTCCGAAGGTAAATCGGAA
ATGAAGAAACTTAGACAGCAGCTTGAGGCAGCTGAGAAACAGATTAAACATTTAGAGATTGAAAAGAATGCTGAAAGTAGCAAGGCTAGTAGCATTACCA
GAGAGCTCCAGGGGAGAGAGCTAAAGCTTACTAACCTTCAGGAAAATTTGAGTGAAGTCAGTCAAGTGAAAGAGACTTTGGAAAAAGAACTTCAGATTTT
G
AAAGAAAAGTTTGCTGAAGCTTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAAGAAACTGTAAATAAGTTACACCAAAAGGAGGAACAGTTTAAC
ATGCTGTCTTCTGACTTGGAG
AAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAATTTAGAGAGAAAGATGAGAGAGAAGAGCAGCTGATAAAGGCAA
AGGAAAAACTGGAAAATGACATTGCAGAAATAATGAAGATGTCAGGAGATAACTCTTCTCAGCTGACAAAAATGAACGATGAATTACGTCTGAAAGAAAG
AGATGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTGCAAAAAAGTATTGAGGACATGACTGTCAAAGCTGAACAGAGC
CAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGAATTGGAGAGGAAATTGTCGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCAGTGTC
AGGAGCTGAAAGCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGAAATCCTACAGAACCTCCAGAAGACGCTGCTGGACACAGAGGA
CAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGGAGCTGAGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAGCGGAA
GATGCCATGCAGATAATGGAACAGATGACCAAAGAGAAGACTGAGACTCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAAACTACAGAATGAAT
TGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGAACTCCTGACTGTAGAGAATCAAAAAATGGAAGAATTTAGGAA
AGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCAGCGTTGCAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGAGAAGC
AGGGACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATCAGTTGTTAGAAATGAAAAAAAGAGAATCCAAGTTCATAAAAG
ACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTATAACTAGTGCCTTACTCACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAATGAGGT
CACAGTGCTCAGGGGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACTCTAGAGTCTGATAAGGTGAAGCTCGAGCTCAAGGTAAAGAAC
TTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAATACAGACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAGTCAGA
TTGATTTCCTAAATTCAGTAATAGTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATGGGAACGG
GGATGACCTAAACAATTATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCTCTTCTGTGACATTTGTGACTGCTTTGATCTCCACGAC
ACAGAGGATTGTCCTACCCAGGCACAGATGTCAGAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAATCTGTG
AGATGTTTGGACACTGGGCCACCAACTGCAATGACGACGAAACCTTCTGATGAAGCCTCCAGTGGAGAACTGGGCTTGCTCAGACGCACTCGCATTGACA
CAACGTAACACCAGCATTGTGTGTGCAGACTTCAGGAGAACTCATGTTATTTTTTAACCCCGTCAACAAATCTAGGAAAATATTTTGATCTTCAACAAAT
TGCCCTTTAGTCTCCCCGTATGAGTTAGAATAATAAATATTTAGTAGGTGAGTTTTCACCTCGAATTTTGTTTTCTTGATTTTTACGTTTGAAGACATTG
CACCAGATGCCATTACATTTATTGGCCCCCCGACCTTGTAGAAAAACCCCTACCCTCACAATACCTTATTTAAGTAACTTTAAATTATGCCGTTACTTTT
CATATTTGCACTAAGATATTTCCAGGCTGCATTTGTATATTTAGATTTTTTGGTTAAGCTTTGACACTGGAATGAGTTGAAAAAATGTGCCATTTTGCAT
TTTCATCTACTCATTTAAAGTATTTTATTCTTATTCAAAGAAATATCTGAGCTCTTTGCACTACCTGTTATCAGTAGTGCCTTTACTTCAGGCTTGATAA
TACTTAGGTGTGATTATAAAATCATGAAGCAGGTAAAGGGAGGGGCAAGCCCCCAAACTGCTGTGGGGACATTTTATAATCTATATGCTGCACCCACTTA
ATCTACTGTGGTGTTTTGTTTATTAGTTTTGCATAATTTCAGCTTCTATATATTGTATGTATATATTTTTTAAAAATCTATATTTTGGGAAAAAAACATA
CACAATGTGTCTTTCTTTTTGGACATTTACCTTTTTGAAAAAGAAAACACTTAAAATGATCATTAGGACATAACAGACTAGGCCAGACATAGCATCTTGT
GGCTTTGCAACCATTTTCATTTGTTTGTTTTCCTTTTATTTCTTCACCAGATTTAAATAACTGGAGGAATTTTCTCCAATTTTTTTTTCCTTCTCTGGCA
GGTATCCCCAGCAGTCAATTAACAATAAGCCAGTATAAAACACCTAAATAACCAATCTACAATCTCCCTTCACAAGTTTTTTTACTGTTTTTAGATGAAT
GTACGATGAGAAATTCAACGTTAATAATTCTGGATTTTCTTATCACAAAAAAGAAAATGAAGGACCTCAAAGCACCTGAACAGTTTATCGACCAGTTTGA
ATCTATTTATCTTCATTTGAATGTCTTCTAGAATATGTAAAAAGTCATAAAATGTATCTTCCATGCTACATGTACAATAAGAACTTCTATAATTGTATAT
ATGCCTTTGATGTATTTTCCCCTCAAGATTATCAACTGTGTGTTCGACAGTGAATATTCAATCTGGTACCAGTTGAAATTTTTGGTTATAAATGTAATAC
GAATTGTTTCACAAACAGAAAACATGTAAAGCAGTATTAAAATTTGGCCAAACAAGTGTTCTGTATCTACTTTAATAAATGGTTATTCTTTTTTGTTT
  • RSN.F10 (forward primer)
  • RSN.R9 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

GGRGGCCCCSGTGGGDRRRPRRFNPETDFTIEP
TVVSMVGER*WQQAKEKQL*HTKKMSMLKPSGLK
APTKILKPGSTALKTPTAVVAPVEKTISSEKAS
STPSSETQEEFVDDFRVGERVWVNGNKPGFIQF
LGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQ
CEPLKGIFTRPSKLTRKVQAEDEANGLQTTPAS
RATSPLCTSTASMVSSSPSTPSNIPQKPSQPAA
KEPSATPPISNLTKTASESISNLSEAGSIKKGER
ELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCG
VELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVH
KVTKIGFPSTTPAKAKANAVRRVMATTSASLKRS
PSASSLSSMSSVASSVSSRPSRTGLLTETSSRY
ARKISGTTALQEALKEKQQHIEQLLAERDLERA
EVAKATSHVGEIEQELALARDGHDQHVLELEAKM
DQLRTMVEAADREKVELLNQLEEEKRKVEDLQF
RVEEESITKGDLEVATVSEKSRIMELEKDLALR
VQEVAELRRRLESNKPAGDVDMSLSLLQEISSLQ
EKLEVTRTDHQREITSLKEHFGAREETHQKEIK
ALYTATEKLSKENESLKSKLEHANKENSDVIAL
WKSKLETAIASHQQAMEELKVSFSKGLGTETAEF
AELKTQIEKMRLDYQHEIENLQNQQDSERAAHA
KEMEALRAKLMKVIKEKENSLEAIRSKLDKAED
QHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQT
KVIDNFTSQLKATEEKLLDLDALRKASSEGKSE
MKKLRQQLEAAEKQIKHLEIEKNAESSKASSIT
RELQGRELKLTNLQENLSEVSQVKETLEKELQIL
KEKFAEASEEAVSVQRSMQETVNKLHQKEEQFN
MLSSDLE
KLRENLADMEAKFREKDEREEQLIKA
KEKLENDIAEIMKMSGDNSSQLTKMNDELRLKER
DVEELQLKLTKANENASFLQKSIEDMTVKAEQS
QQEAAKKHEEEKKELERKLSDLEKKMETSHNQC
QELKARYERATSETKTKHEEILQNLQKTLLDTED
KLKGAREENSGLLQELEELRKQADKAKAAQTAE
DAMQIMEQMTKEKTETLASLEDTKQTNAKLQNE
LDTLKENNLKNVEELNKSKELLTVENQKMEEFRK
EIETLKQAAAQKSQQLSALQEENVKLAEELGRS
RDEVTSHQKLEEERSVLNNQLLEMKKRESKFIK
DADEEKASLQKSISITSALLTEKDAELEKLRNEV
TVLRGENASAKSLHSVVQTLESDKVKLELKVKN
LELQLKENKRQLSSSSGNTDTQADEDERAQESQ
IDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNG
DDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHD
TEDCPTQAQMSEDPPHSTHHGSRGEERPYCEIC
EMFGHWATNCNDDETF**SLQWRTGLAQTHSH*H
NVTPALCVQTSGELMLFFNPVNKSRKIF*SSTN
CPLVSPYELE**IFSR*VFTSNFVFLIFTFEDI
APDAITFIGPPTL*KNPYPHNTLFK*L*IMPLLF
IFALRYFQAAFVYLDFLVKL*HWNELKKCAILH
FHLLI*SILFLFKEISELFALPVISSAFTSGLI
ILRCDYKIMKQVKGGASPQTAVGTFYNLYAAPT*
STVVFCLLVLHNFSFYILYVYIF*KSIFWEKNI
HNVSFFLDIYLFEKENT*NDH*DITD*ARHSIL
WLCNHFHLFVFLLFLHQI*ITGGIFSNFFFLLWQ
VSPAVN*Q*ASIKHLNNQSTISLHKFFYCF*MN
VR*EIQR**FWIFLSQKRK*RTSKHLNSLSTSL
NLFIFI*MSSRICKKS*NVSSMLHVQ*ELL*LYI
CL*CIFPSRLSTVCSTVNIQSGTS*NFWL*M*Y
ELFHKQKTCKAVLKFGQTSVLYLL**MVILFC

Frame 1

AAAAAAAAAVGQVAATGGVRGDLIQRLTSL*NP
QLYQWLGKDSGNRQRRNSSDIQRK*VC*SQVGL
RPPPRS*SLEAQL*RHLRLL*LQ*KKPYPVKKHQ
ALHHLRLRRNLWMTFELGSEFG*MEISLDLSSF
LEKPSLHQASGLELF*MNP*ARTMVRWQEFGIS
SVNL*RAYLPDLQS*QGRCKQKMKLMACRQRPPP
ELLHRCALLRPAWCLPPPPPLQTSLRNHHSQQQ
RNLQLRLRSATLQKLPVNLSPTFQRLAQSRKEK
ESSKSETEYWLVALRLV*SGFLGRPTLPRGSGVA
WS*MSHLGRMMALLLEQGIFSVNPNMACSLLST
KLPRLASLPLHQPKPRPTQ*GE*WRPRPPA*SA
ALLPLPSAP*AQWPPL*AAGPVGQDY*LKPPPVT
PGRSPVPLPSRRP*RRSSSTLSSCWRNGIWRGR
RWPRPRATWGR*SRS*LWPGTDMTSMSWNWKPK
WTSCEQWWKLLTGRRWSFSTSLKRRKGRLRTFSS
GLKKNQLPKVILRWLQFQKSHV*WNWRKT*H*E
YRK*LSSEEG*SPISLLGMWTCHFPFCKR*ALC
KKS*KSPVLTTREK*LL*RSILEPGKKLIRRR*R
LCIPPRKSFPKRTSH*KASWSMPTKRTQM**LY
GSPNWRLPSHPTSRRWKN*RYLSAKGLEQRRQN
LLN*KHK*RK*D*ITNTK*KICRINKTLNGLPML
KRWKP*GLN**KLLKKRKTVWKPSGRNWTKQKT
SIS*KWKTR*TNYRKLK*R*RS*RYCKPNAMNK
PRLLIILHHSSRLLKKSSWILMHFGKPVPKVNRK
*RNLDSSLRQLRNRLNI*RLKRMLKVARLVALP
ESSRGES*SLLTFRKI*VKSVK*KRLWKKNFRF
*
KKSLLKLQRRQSLFREVCKKL*ISYTKRRNSLT
CCLLTWR
S*EKT*QIWRQNLERKMREKSS**RQ
RKNWKMTLQK**RCQEITLLS*QK*TMNYV*KK
EM*KNYS*NLQRLMKMQVFCKKVLRT*LSKLNRA
SKKQLKSMRKKRKNWRGNCRTWKRKWKQATTSV
RS*KPGMREPLLRQKPSMKKSYRTSRRRCWTQR
TS*RAHGRRTVACCRSWRS*ESKPTKPKLLKQRK
MPCR*WNR*PKRRLRLWPPWRTPSKQMQNYRMN
WTHLKKTT*KMWKS*TNQKNS*L*RIKKWKNLG
KK*KP*SRQQLRSPSSFQRCKKRTLNLLRSWGEA
GTKSQVIKSWKKKDLCSIISC*K*KKENPSS*K
TQMKRKLPCRNPSV*LVPYSQKRMPSWRN*EMR
SQCSGEKTPLPSPCIQLFRL*SLIR*SSSSR*RT
WSFNSKKTRGSSAAPQVIQTLRQTRMKEPRRVR
LIS*IQ**WTFKGRIKTSR*RWR*CQKQP*MGT
GMT*TIMTVMIRRNSPRRNLASSVTFVTALISTT
QRIVLPRHRCQRTLPIPHTMAVGVRNAHTVKSV
RCLDTGPPTAMTTKPSDEASSGELGLLRRTRID
TT*HQHCVCRLQENSCYFLTPSTNLGKYFDLQQI
AL*SPRMS*NNKYLVGEFSPRILFS*FLRLKTL
HQMPLHLLAPRPCRKTPTLTIPYLSNFKLCRYF
SYLH*DISRLHLYI*IFWLSFDTGMS*KNVPFCI
FIYSFKVFYSYSKKYLSSLHYLLSVVPLLQA**
YLGVIIKS*SR*REGQAPKLLWGHFIIYMLHPL
NLLWCFVY*FCIISASIYCMYIFFKNLYFGKKTY
TMCLSFWTFTFLKKKTLKMIIRT*QTRPDIASC
GFATIFICLFSFYFFTRFK*LEEFSPIFFSFSG
RYPQQSINNKPV*NT*ITNLQSPFTSFFTVFR*M
YDEKFNVNNSGFSYHKKENEGPQST*TVYRPV*
IYLSSFECLLEYVKSHKMYLPCYMYNKNFYNCI
YAFDVFSPQDYQLCVRQ*IFNLVPVEIFGYKCNT
NCFTNRKHVKQY*NLAKQVFCIYFNKWLFFFV

Frame 2

RPRRLLLLQWDRWRRPAASEEI*SRD*LHYRTH
SCINGWGKIVATGKGETALTYKENEYAKAKWA*
GPHQDPEAWKHSSEDTYGCCSSSRKNHIQ*KSI
KHSII*DSGGICG*LSSWGASLGEWK*AWIYPVS
WRNPVCTRPVGWNCFR*THRQERWFGGRSSVFP
V*TFKGHIYPTFKVNKEGASRR*S*WPADNARL
PSYFTAVHFYGQHGVFLPLHPFKHPSETITASSK
GTFSYASDQQPYKNCQ*IYLQPFRGWLNQERRK
RAQNRRQSIGWWH*GWCSPVSWGDRLCQGGVVW
RGVR*ATWEE*WRCCWNKVFSVSTQIWLVRSCPQ
SYQDWLPFHYTSQSQGQRSEASDGDHVRQPEAQ
PFCLFPQLHELSGLLCEQQAQSDRTID*NLLPL
RQEDLRYHCPPGGPEGEAAAH*AAAGGTGSGEGG
GGQGHEPRGGDRAGASSGPGRT*PACPGIGSQN
GPAANNGGSC*QGEGGASQPA*RGEKEG*GPSV
PG*RRINYQR*S*GGYSFRKVTYNGTGERPSIES
TGSS*APKKARVQ*ACWGCGHVTFPFARDKLFA
RKVRSHPY*PPERNNFSEGAFWSPGRNSSEGDK
GSVYRHGKAFQRERVIEKQAGACQQRELRCDSSM
EVQTGDCHRIPPAGDGRTEGIFQQRAWNRDGRI
C*TKNTNRENETRLPTRNRKFAESTRL*TGCPC
*RDGSLEG*TDESY*RKGKQSGSHQVETGQSRRP
ASRRNGRHVKQITGS*NKGKGARGTASQMQ*TN
QGY**FYITAQGY*RKALGS*CTSESQFRR*IG
NEET*TAA*GS*ETD*TFRD*KEC*K*QG**HYQ
RAPGERAKAY*PSGKFE*SQSSERDFGKRTSDF
ERKVC*SFRGGSLCSEKYARNCK*VTPKGGTV*
HAVF*LG
EAERKLSRYGGKI*RER*ERRAADKGK
GKTGK*HCRNNEDVRR*LFSADKNER*ITSERK
RCRRITAKTYKG**KCKFSAKKY*GHDCQS*TE
PARSS*KA*GRKERIGEEIVGPGKENGNKPQPVS
GAESQV*ESHF*DKNQA*RNPTEPPEDAAGHRG
QAEGRTGGEQWLAAGAGGAEKASRQSQSCSNSG
RCHADNGTDDQRED*DSGLLGGHQANKCKTTE*I
GHT*RKQLEKCGRAEQIKRTPDCRESKNGRI*E
RNRNPKAGSSSEVPAAFSVARRER*TC*GAGEK
QGRSHKSSKAGRRKICAQ*SVVRNEKKRIQVHKR
RR*RESFLAEIHQYN*CLTHRKGCRAGETEK*G
HSAQGRKRLCQVLAFSCSDSRV**GEARAQGKE
LGASTQRKQEAAQQLLR*YRHSGRRG*KSPGESD
*FPKFSNSGPSKEESRPQDEGGDDVRSSPEWER
G*PKQL*Q**SGETVQEETSPLL*HL*LL*SPR
HRGLSYPGTDVRGPSPFHTPWQSG*GTPIL*NL*
DVWTLGHQLQ*RRNLLMKPPVENWACSDALALT
QRNTSIVCADFRRTHVIF*PRQQI*ENILIFNK
LPFSLPV*VRIINI**VSFHLEFCFLDFYV*RHC
TRCHYIYWPPDLVEKPLPSQYLI*VTLNYAVTF
HICTKIFPGCICIFRFFG*ALTLE*VEKMCHFA
FSSTHLKYFILIQRNI*ALCTTCYQ*CLYFRLDN
T*V*L*NHEAGKGRGKPPNCCGDIL*SICCTHL
IYCGVLFISFA*FQLLYIVCIYFLKIYILGKKH
TQCVFLFGHLPF*KRKHLK*SLGHNRLGQT*HLV
ALQPFSFVCFPFISSPDLNNWRNFLQFFFPSLA
GIPSSQLTISQYKTPK*PIYNLPSQVFLLFLDE
CTMRNSTLIILDFLITKKKMKDLKAPEQFIDQFE
SIYLHLNVF*NM*KVIKCIFHATCTIRTSIIVY
MPLMYFPLKIINCVFDSEYSIWYQLKFLVINVI
RIVSQTENM*SSIKIWPNKCSVSTLINGYSFLF

Sequence RSN.aAug05

GGCGGCCGCGGCGGCTGCTGCTGCTGCAGTGGGACAGGTGGCGGCGACCGGCGGCGTCCGAGGAGATTTAATCCAGAGACTGACTTCACTATAGAACCCA
CAGTTGTATCAATGGTTGGGGAAAGATAGTGGCAACAGGCAAAGGAGAAACAGCTCTGACATACAAAGAAAATGAGTATGCTAAAGCCAAGTGGGCTTAA
GGCCCCCACCAAGATCCTGAAGCCTGGAAGCACAGCTCTGAAGACACCTACGGCTGTTGTAGCTCCAGTAGAAAAAACCATATCCAGTGAAAAAGCATCA
AGCACTCCATCATCTGAGACTCAGGAGGAATTTGTGGATGACTTTCGAGTTGGGGAGCGAGTTTGGGTGAATGGAAATAAGCCTGGATTTATCCAGTTTC
TTGGAGAAACCCAGTTTGCACCAGGCCAGTGGGCTGGAATTGTTTTAGATGAACCCATAGGCAAGAACGATGGTTCGGTGGCAGGAGTTCGGTATTTCCA
GTGTGAACCTTTAAAGGGCATATTTACCCGACCTTCAAAGTTAACAAGGAAGGTGCAAGCAGAAGATGAAGCTAATGGCCTGCAGACAACGCCCGCCTCC
CGAGCTACTTCACCGCTGTGCACTTCTACGGCCAGCATGGTGTCTTCCTCCCCCTCCACCCCTTCAAACATCCCTCAGAAACCATCACAGCCAGCAGCAA
AGGAACCTTCAGCTACGCCTCCGATCAGCAACCTTACAAAAACTGCCAGTGAATCTATCTCCAACCTTTCAGAGGCTGGCTCAATCAAGAAAGGAGAAAG
AGAGCTCAAAATCGGAGACAGAGTATTGGTTGGTGGCACTAAGGCTGGTGTAGTCCGGTTTCTTGGGGAGACCGACTTTGCCAAGGGGGAGTGGTGTGGC
GTGGAGTTAGATGAGCCACTTGGGAAGAATGATGGCGCTGTTGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACA
AAGTTACCAAGATTGGCTTCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGTCCGCCAGCCTGAAGCGCAG
CCCTTCTGCCTCTTCCCTCAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAGCAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTAC
GCCAGGAAGATCTCCGGTACCACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGGAGAGGGCGG
AGGTGGCCAAGGCCACGAGCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGACGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAAT
GGACCAGCTGCGAACAATGGTGGAAGCTGCTGACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTC
CGGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGACGCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTTGAACAGAGCCTGCTCTTTGAAA
AGACCAAAGCTGACAAACTCCAGAGGGAGTTAGAAGACACTAGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCATT
GAGAGTACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAATAAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCT
TTGCAAGAAAAGTTAGAAGTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTCATCAGAAGGAGA
TAAAGGCTCTGTATACCGCCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGC
TCTATGGAAGTCCAAACTGGAGACTGCCATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCA
GAATTTGCTGAACTAAAAACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTTGCAGAATCAACAAGACTCTGAACGGGCTGCCC
ATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGATGAAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGA
AGACCAGCATCTCGTAGAAATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGGTAAAGGAGCTAGAGGTACTGCAAGCCAAATGCAATGAA
CAAACCAAGGTTATTGATAATTTTACATCACAGCTCAAGGCTACTGAAGAAAAGCTCTTGGATCTTGATGCACTTCGGAAAGCCAGTTCCGAAGGTAAAT
CGGAAATGAAGAAACTTAGACAGCAGCTTGAGGCAGCTGAGAAACAGATTAAACATTTAGAGATTGAAAAGAATGCTGAAAGTAGCAAGGCTAGTAGCAT
TACCAGAGAGCTCCAGGGGAGAGAGCTAAAGCTTACTAACCTTCAGGAAAATTTGAGTGAAGTCAGTCAAGTGAAAGAGACTTTGGAAAAAGAACTTCAG
ATTTTG
AAAGAAAAGTTTGCTGAAGCTTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAAGAAACTGTAAATAAGTTACACCAAAAGGAGGAACAGT
TTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAATTTAGAGAGAAAGATGAGAGAGAAGAGCAGCTGATAAA
GGCAAAGGAAAAACTGGAAAATGACATTGCAGAAATAATGAAGATGTCAGGAGATAACTCTTCTCAGCTGACAAAAATGAACGATGAATTACGTCTGAAA
GAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTGCAAAAAAGTATTGAGGACATGACTGTCAAAGCTGAAC
AGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGAATTGGAGAGGAAATTGTCGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCA
GTGTCAGGAGCTGAAAGCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGAAATCCTACAGAACCTCCAGAAGACGCTGCTGGACACA
GAGGACAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGGAGCTGAGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAG
CGGAAGATGCCATGCAGATAATGGAACAGATGACCAAAGAGAAGACTGAGACTCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAAACTACAGAA
TGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGAACTCCTGACTGTAGAGAATCAAAAAATGGAAGAATTT
AGGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCAGCGTTGCAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGA
GAAGCAGGGACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATCAGTTGTTAGAAATGAAAAAAAGAGAATCCAAGTTCAT
AAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTATAACTAGTGCCTTACTCACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAAT
GAGGTCACAGTGCTCAGGGGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACTCTAGAGTCTGATAAGGTGAAGCTCGAGCTCAAGGTAA
AGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAATACAGACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAG
TCAGATTGATTTCCTAAATTCAGTAATAGTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATGGG
AACGGGGATGACCTAAACAATTATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCTCTTCTGTGACATTTGTGACTGCTTTGATCTCC
ACGACACAGAGGATTGTCCTACCCAGGCACAGATGTCAGAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAAT
CTGTGAGATGTTTGGACACTGGGCCACCAACTGCAATGACGACGAAACCTTCTGATGAAGCCTCCAGTGGAGAACTGGGCTTGCTCAGACGCACTCGCAT
TGACACAACGTAACACCAGCATTGTGTGTGCAGACTTCAGGAGAACTCATGTTATTTTTTAACCCCGTCAACAAATCTAGGAAAATATTTTGATCTTCAA
CAAATTGCCCTTTAGTCTCCCCGTATGAGTTAGAATAATAAATATTTAGTAGGTGAGTTTTCACCTCGAATTTTGTTTTCTTGATTTTTACGTTTGAAGA
CATTGCACCAGATGCCATTACATTTATTGGCCCCCCGACCTTGTAGAAAAACCCCTACCCTCACAATACCTTATTTAAGTAACTTTAAATTATGCCGTTA
CTTTTCATATTTGCACTAAGATATTTCCAGGCTGCATTTGTATATTTAGATTTTTTGGTTAAGCTTTGACACTGGAATGAGTTGAAAAAATGTGCCATTT
TGCATTTTCATCTACTCATTTAAAGTATTTTATTCTTATTCAAAGAAATATCTGAGCTCTTTGCACTACCTGTTATCAGTAGTGCCTTTACTTCAGGCTT
GATAATACTTAGGTGTGATTATAAAATCATGAAGCAGGTAAAGGGAGGGGCAAGCCCCCAAACTGCTGTGGGGACATTTTATAATCTATATGCTGCACCC
ACTTAATCTACTGTGGTGTTTTGTTTATTAGTTTTGCATAATTTCAGCTTCTATATATTGTATGTATATATTTTTTAAAAATCTATATTTTGGGAAAAAA
ACATACACAATGTGTCTTTCTTTTTGGACATTTACCTTTTTGAAAAAGAAAACACTTAAAATGATCATTAGGACATAACAGACTAGGCCAGACATAGCAT
CTTGTGGCTTTGCAACCATTTTCATTTGTTTGTTTTCCTTTTATTTCTTCACCAGATTTAAATAACTGGAGGAATTTTCTCCAATTTTTTTTTCCTTCTC
TGGCAGGTATCCCCAGCAGTCAATTAACAATAAGCCAGTATAAAACACCTAAATAACCAATCTACAATCTCCCTTCACAAGTTTTTTTACTGTTTTTAGA
TGAATGTACGATGAGAAATTCAACGTTAATAATTCTGGATTTTCTTATCACAAAAAAGAAAATGAAGGACCTCAAAGCACCTGAACAGTTTATCGACCAG
TTTGAATCTATTTATCTTCATTTGAATGTCTTCTAGAATATGTAAAAAGTCATAAAATGTATCTTCCATGCTACATGTACAATAAGAACTTCTATAATTG
TATATATGCCTTTGATGTATTTTCCCCTCAAGATTATCAACTGTGTGTTCGACAGTGAATATTCAATCTGGTACCAGTTGAAATTTTTGGTTATAAATGT
AATACGAATTGTTTCACAAACAGAAAACATGTAAAGCAGTATTAAAATTTGGCCAAACAAGTGTTCTGTATCTACTTTAATAAATGGTTATTCTTTTTTG
TTT
  • RSN.F10 (forward primer)
  • RSN.R9 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

GGRGGCCCCSGTGGGDRRRPRRFNPETDFTIEP
TVVSMVGER*WQQAKEKQL*HTKKMSMLKPSGLK
APTKILKPGSTALKTPTAVVAPVEKTISSEKAS
STPSSETQEEFVDDFRVGERVWVNGNKPGFIQF
LGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQ
CEPLKGIFTRPSKLTRKVQAEDEANGLQTTPAS
RATSPLCTSTASMVSSSPSTPSNIPQKPSQPAA
KEPSATPPISNLTKTASESISNLSEAGSIKKGER
ELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCG
VELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVH
KVTKIGFPSTTPAKAKANAVRRVMATTSASLKRS
PSASSLSSMSSVASSVSSRPSRTGLLTETSSRY
ARKISGTTALQEALKEKQQHIEQLLAERDLERA
EVAKATSHVGEIEQELALARDGHDQHVLELEAKM
DQLRTMVEAADREKVELLNQLEEEKRKVEDLQF
RVEEESITKGDLETQTKLEHARIKELEQSLLFE
KTKADKLQRELEDTRVATVSEKSRIMELEKDLAL
RVQEVAELRRRLESNKPAGDVDMSLSLLQEISS
LQEKLEVTRTDHQREITSLKEHFGAREETHQKE
IKALYTATEKLSKENESLKSKLEHANKENSDVIA
LWKSKLETAIASHQQAMEELKVSFSKGLGTETA
EFAELKTQIEKMRLDYQHEIENLQNQQDSERAA
HAKEMEALRAKLMKVIKEKENSLEAIRSKLDKAE
DQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNE
QTKVIDNFTSQLKATEEKLLDLDALRKASSEGK
SEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSI
TRELQGRELKLTNLQENLSEVSQVKETLEKELQ
IL
KEKFAEASEEAVSVQRSMQETVNKLHQKEEQ
FNMLSSDLEKLRENLADMEAKFREKDEREEQLIK
AKEKLENDIAEIMKMSGDNSSQLTKMNDELRLK
ERDVEELQLKLTKANENASFLQKSIEDMTVKAE
QSQQEAAKKHEEEKKELERKLSDLEKKMETSHNQ
CQELKARYERATSETKTKHEEILQNLQKTLLDT
EDKLKGAREENSGLLQELEELRKQADKAKAAQT
AEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQN
ELDTLKENNLKNVEELNKSKELLTVENQKMEEF
RKEIETLKQAAAQKSQQLSALQEENVKLAEELG
RSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFI
KDADEEKASLQKSISITSALLTEKDAELEKLRN
EVTVLRGENASAKSLHSVVQTLESDKVKLELKV
KNLELQLKENKRQLSSSSGNTDTQADEDERAQES
QIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNG
NGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDL
HDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEI
CEMFGHWATNCNDDETF**SLQWRTGLAQTHSH
*HNVTPALCVQTSGELMLFFNPVNKSRKIF*SS
TNCPLVSPYELE**IFSR*VFTSNFVFLIFTFED
IAPDAITFIGPPTL*KNPYPHNTLFK*L*IMPL
LFIFALRYFQAAFVYLDFLVKL*HWNELKKCAI
LHFHLLI*SILFLFKEISELFALPVISSAFTSGL
IILRCDYKIMKQVKGGASPQTAVGTFYNLYAAP
T*STVVFCLLVLHNFSFYILYVYIF*KSIFWEK
NIHNVSFFLDIYLFEKENT*NDH*DITD*ARHSI
LWLCNHFHLFVFLLFLHQI*ITGGIFSNFFFLL
WQVSPAVN*Q*ASIKHLNNQSTISLHKFFYCF*
MNVR*EIQR**FWIFLSQKRK*RTSKHLNSLSTS
LNLFIFI*MSSRICKKS*NVSSMLHVQ*ELL*L
YICL*CIFPSRLSTVCSTVNIQSGTS*NFWL*M
*YELFHKQKTCKAVLKFGQTSVLYLL**MVILFC

Frame 1

AAAAAAAAAVGQVAATGGVRGDLIQRLTSL*NP
QLYQWLGKDSGNRQRRNSSDIQRK*VC*SQVGL
RPPPRS*SLEAQL*RHLRLL*LQ*KKPYPVKKHQ
ALHHLRLRRNLWMTFELGSEFG*MEISLDLSSF
LEKPSLHQASGLELF*MNP*ARTMVRWQEFGIS
SVNL*RAYLPDLQS*QGRCKQKMKLMACRQRPPP
ELLHRCALLRPAWCLPPPPPLQTSLRNHHSQQQ
RNLQLRLRSATLQKLPVNLSPTFQRLAQSRKEK
ESSKSETEYWLVALRLV*SGFLGRPTLPRGSGVA
WS*MSHLGRMMALLLEQGIFSVNPNMACSLLST
KLPRLASLPLHQPKPRPTQ*GE*WRPRPPA*SA
ALLPLPSAP*AQWPPL*AAGPVGQDY*LKPPPVT
PGRSPVPLPSRRP*RRSSSTLSSCWRNGIWRGR
RWPRPRATWGR*SRS*LWPGTDMTSMSWNWKPK
WTSCEQWWKLLTGRRWSFSTSLKRRKGRLRTFSS
GLKKNQLPKVILRRRPNWSMPALRSLNRACSLK
RPKLTNSRGS*KTLGWLQFQKSHV*WNWRKT*H
*EYRK*LSSEEG*SPISLLGMWTCHFPFCKR*AL
CKKS*KSPVLTTREK*LL*RSILEPGKKLIRRR
*RLCIPPRKSFPKRTSH*KASWSMPTKRTQM**
LYGSPNWRLPSHPTSRRWKN*RYLSAKGLEQRRQ
NLLN*KHK*RK*D*ITNTK*KICRINKTLNGLP
MLKRWKP*GLN**KLLKKRKTVWKPSGRNWTKQ
KTSIS*KWKTR*TNYRKLK*R*RS*RYCKPNAMN
KPRLLIILHHSSRLLKKSSWILMHFGKPVPKVN
RK*RNLDSSLRQLRNRLNI*RLKRMLKVARLVA
LPESSRGES*SLLTFRKI*VKSVK*KRLWKKNFR
F*
KKSLLKLQRRQSLFREVCKKL*ISYTKRRNS
LTCCLLTWRS*EKT*QIWRQNLERKMREKSS**
RQRKNWKMTLQK**RCQEITLLS*QK*TMNYV*K
KEM*KNYS*NLQRLMKMQVFCKKVLRT*LSKLN
RASKKQLKSMRKKRKNWRGNCRTWKRKWKQATT
SVRS*KPGMREPLLRQKPSMKKSYRTSRRRCWTQ
RTS*RAHGRRTVACCRSWRS*ESKPTKPKLLKQ
RKMPCR*WNR*PKRRLRLWPPWRTPSKQMQNYR
MNWTHLKKTT*KMWKS*TNQKNS*L*RIKKWKNL
GKK*KP*SRQQLRSPSSFQRCKKRTLNLLRSWG
EAGTKSQVIKSWKKKDLCSIISC*K*KKENPSS
*KTQMKRKLPCRNPSV*LVPYSQKRMPSWRN*EM
RSQCSGEKTPLPSPCIQLFRL*SLIR*SSSSR*
RTWSFNSKKTRGSSAAPQVIQTLRQTRMKEPRR
VRLIS*IQ**WTFKGRIKTSR*RWR*CQKQP*MG
TGMT*TIMTVMIRRNSPRRNLASSVTFVTALIS
TTQRIVLPRHRCQRTLPIPHTMAVGVRNAHTVK
SVRCLDTGPPTAMTTKPSDEASSGELGLLRRTRI
DTT*HQHCVCRLQENSCYFLTPSTNLGKYFDLQ
QIAL*SPRMS*NNKYLVGEFSPRILFS*FLRLK
TLHQMPLHLLAPRPCRKTPTLTIPYLSNFKLCRY
FSYLH*DISRLHLYI*IFWLSFDTGMS*KNVPF
CIFIYSFKVFYSYSKKYLSSLHYLLSVVPLLQA
**YLGVIIKS*SR*REGQAPKLLWGHFIIYMLHP
LNLLWCFVY*FCIISASIYCMYIFFKNLYFGKK
TYTMCLSFWTFTFLKKKTLKMIIRT*QTRPDIA
SCGFATIFICLFSFYFFTRFK*LEEFSPIFFSFS
GRYPQQSINNKPV*NT*ITNLQSPFTSFFTVFR
*MYDEKFNVNNSGFSYHKKENEGPQST*TVYRP
V*IYLSSFECLLEYVKSHKMYLPCYMYNKNFYNC
IYAFDVFSPQDYQLCVRQ*IFNLVPVEIFGYKC
NTNCFTNRKHVKQY*NLAKQVFCIYFNKWLFFF
V

Frame 2

RPRRLLLLQWDRWRRPAASEEI*SRD*LHYRTH
SCINGWGKIVATGKGETALTYKENEYAKAKWA*
GPHQDPEAWKHSSEDTYGCCSSSRKNHIQ*KSI
KHSII*DSGGICG*LSSWGASLGEWK*AWIYPVS
WRNPVCTRPVGWNCFR*THRQERWFGGRSSVFP
V*TFKGHIYPTFKVNKEGASRR*S*WPADNARL
PSYFTAVHFYGQHGVFLPLHPFKHPSETITASSK
GTFSYASDQQPYKNCQ*IYLQPFRGWLNQERRK
RAQNRRQSIGWWH*GWCSPVSWGDRLCQGGVVW
RGVR*ATWEE*WRCCWNKVFSVSTQIWLVRSCPQ
SYQDWLPFHYTSQSQGQRSEASDGDHVRQPEAQ
PFCLFPQLHELSGLLCEQQAQSDRTID*NLLPL
RQEDLRYHCPPGGPEGEAAAH*AAAGGTGSGEGG
GGQGHEPRGGDRAGASSGPGRT*PACPGIGSQN
GPAANNGGSC*QGEGGASQPA*RGEKEG*GPSV
PG*RRINYQR*S*DADQTGACPH*GA*TEPAL*K
DQS*QTPEGVRRH*GGYSFRKVTYNGTGERPSI
ESTGSS*APKKARVQ*ACWGCGHVTFPFARDKL
FARKVRSHPY*PPERNNFSEGAFWSPGRNSSEGD
KGSVYRHGKAFQRERVIEKQAGACQQRELRCDS
SMEVQTGDCHRIPPAGDGRTEGIFQQRAWNRDG
RIC*TKNTNRENETRLPTRNRKFAESTRL*TGCP
C*RDGSLEG*TDESY*RKGKQSGSHQVETGQSR
RPASRRNGRHVKQITGS*NKGKGARGTASQMQ*
TNQGY**FYITAQGY*RKALGS*CTSESQFRR*I
GNEET*TAA*GS*ETD*TFRD*KEC*K*QG**H
YQRAPGERAKAY*PSGKFE*SQSSERDFGKRTS
DF
ERKVC*SFRGGSLCSEKYARNCK*VTPKGGTV
*HAVF*LGEAERKLSRYGGKI*RER*ERRAADK
GKGKTGK*HCRNNEDVRR*LFSADKNER*ITSE
RKRCRRITAKTYKG**KCKFSAKKY*GHDCQS*T
EPARSS*KA*GRKERIGEEIVGPGKENGNKPQP
VSGAESQV*ESHF*DKNQA*RNPTEPPEDAAGH
RGQAEGRTGGEQWLAAGAGGAEKASRQSQSCSNS
GRCHADNGTDDQRED*DSGLLGGHQANKCKTTE
*IGHT*RKQLEKCGRAEQIKRTPDCRESKNGRI
*ERNRNPKAGSSSEVPAAFSVARRER*TC*GAGE
KQGRSHKSSKAGRRKICAQ*SVVRNEKKRIQVH
KRRR*RESFLAEIHQYN*CLTHRKGCRAGETEK
*GHSAQGRKRLCQVLAFSCSDSRV**GEARAQGK
ELGASTQRKQEAAQQLLR*YRHSGRRG*KSPGE
SD*FPKFSNSGPSKEESRPQDEGGDDVRSSPEW
ERG*PKQL*Q**SGETVQEETSPLL*HL*LL*SP
RHRGLSYPGTDVRGPSPFHTPWQSG*GTPIL*N
L*DVWTLGHQLQ*RRNLLMKPPVENWACSDALA
LTQRNTSIVCADFRRTHVIF*PRQQI*ENILIFN
KLPFSLPV*VRIINI**VSFHLEFCFLDFYV*R
HCTRCHYIYWPPDLVEKPLPSQYLI*VTLNYAV
TFHICTKIFPGCICIFRFFG*ALTLE*VEKMCHF
AFSSTHLKYFILIQRNI*ALCTTCYQ*CLYFRL
DNT*V*L*NHEAGKGRGKPPNCCGDIL*SICCT
HLIYCGVLFISFA*FQLLYIVCIYFLKIYILGKK
HTQCVFLFGHLPF*KRKHLK*SLGHNRLGQT*H
LVALQPFSFVCFPFISSPDLNNWRNFLQFFFPS
LAGIPSSQLTISQYKTPK*PIYNLPSQVFLLFLD
ECTMRNSTLIILDFLITKKKMKDLKAPEQFIDQ
FESIYLHLNVF*NM*KVIKCIFHATCTIRTSII
VYMPLMYFPLKIINCVFDSEYSIWYQLKFLVINV
IRIVSQTENM*SSIKIWPNKCSVSTLINGYSFL
F

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