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Sequences

Sequence SAT.iAug05

AAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACTGAGAAGAGGACGCTCCC GGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAAATGGCTAAATTCGTGAT CCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGTAGCGGAGAGCCAGAGCTCCTCCCGGGGCGTGGGGTGGAGGTGGCTCCGT TTCCCAGAGTGGGGTGATTCACGTCTTGAGGTCTCGGCCGGTGTGAGAGCCCAGCCTGTTCCCCTTTCTGCGCCCATCCCCGGCTCGGCCGCCACCCCGC CCGCCCGCCCCATTCCGTTCCCCTGAGCTGGCCGGGCCGGAGCCTCATTTTGTTTTCTACTTTTTTCTCTCCTATGTGCATCCCCCCAGGAGCTGGCTAA ATATGAATACATGGAAGAACAAGTAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTG CCGAAAGAGCACTGGACTCCGGAAGGACACAGCATTGTTGGTTTTGCCATGTACTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCG TGATGAGTGATTATAGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGT AGCAGAATGGAATGAACCATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAG TACTTGCTAAAAATGGCAACAGAGGAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATG CTGTTTGTAGTGAAATAATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCT CAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGT GAGTCATTTAAATGTGTACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

KCAALSRGPTGVYPSLAEVPWVMVPA*LRRGRS RETNEEPPPPTVQVQGPGPQREEKQKTKMAKFVI RPATAADCSDILRLIKVAESQSSSRGVGWRWLR FPEWGDSRLEVSAGVRAQPVPLSAPIPGSAATP PARPIPFP*AGRAGASFCFLLFSLLCASPQELAK YEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEV PKEHWTPEGHSIVGFAMYYDPWIGKLLYLEDFF VMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLV AEWNEPSINFYKRRGASDLSSEEGWRLFKIDKE YLLKMATEE*GVLL*MTTSILF*NKFPTSLAFY AVCSEIIE*APIPKLYYQWRCCMFEMRSV*SGNL RCSLESQIFLLEYLSINNKVV*S*YI*KKLHSR ESFKCVQCTHWYLEFLFDSFLINYSLI*LFGVS

Frame 1

NAQLLVAGRLVFIRHSPRFLGSWCQPD*EEDAP GRRMRNHLLLLFKYRGLVRKGKKSKRRKWLNS* SAQPLPPTAVTYCG*SR*RRARAPPGAWGGGGSV SQSGVIHVLRSRPV*EPSLFPFLRPSPARPPPR PPAPFRSPELAGPEPHFVFYFFLSYVHPPRSWL NMNTWKNK*S*LKKIC*KMVLESTPFTTAWLQKC RKSTGLRKDTALLVLPCTMTRGLASYCILRTSS **VIIEALA*DQKF*RI*ARLQ*GVAAAACTSW *QNGMNHPSTSIKEEVLLICPVKRVGDCSRSTRS TC*KWQQRSEECCCR*QPPFYFRINSQLLLLSM LFVVK**NEHPFQSFITSGVVACLK*GLFKVAI SDAVWRVRSFSLNIFR*TTRWCDLNIFEKNFILV SHLNVYNVHTGT*SFCLILF**TTL*FNCLV*A

Frame 2

MRSS*SRADWCLSVTRRGSLGHGASLTEKRTLP GDE*GTTSSYCSSTGAWSAKGRKAKDENG*IRD PPSHCRRLQ*HTAADQGSGEPELLPGRGVEVAP FPRVG*FTS*GLGRCESPACSPFCAHPRLGRHPA RPPHSVPLSWPGRSLILFSTFFSPMCIPPGAG* I*IHGRTSNLN*KRSARRWFWRAPLLPLPGCRS AERALDSGRTQHCWFCHVL*PVDWQVIVS*GLLR DE*L*RLWHRIRNSEESKPGCNEVSLQQHALLG SRME*TIHQLL*KKRCF*SVQ*RGLETVQDRQG VLAKNGNRGVRSAAVDDNLHSILE*IPNFSCFLC CL**NNRMSTHSKALLPVALLHV*NEVCLKWQS QMQFGESDLSP*ISFDKQQGGVILIYLKKTSFS *VI*MCTMYTLVLRVSV*FFFNKLLFDLIVWCK

Sequence SAT.eAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KDTALLVLPCTILPMTRGLASYCILRTSS**VII EALA*DQKF*RI*ARLQ*GVAAAACTSW*QNGM NHPSTSIKEEVLLICPVKRVGDCSRSTRSTCYF RINSQLLLLSMLFVVK**NEHPFQSFITSGVVAC LK*GLFKVAISDAVWRVRSFSLNIFR*TTRWCD LNIFEKNFILVSHLNVYNVHTGT*SFCLILF** TTL*FNCLV*A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG RTQHCWFCHVLFYL*PVDWQVIVS*GLLRDE*L *RLWHRIRNSEESKPGCNEVSLQQHALLGSRME* TIHQLL*KKRCF*SVQ*RGLETVQDRQGVLAIL E*IPNFSCFLCCL**NNRMSTHSKALLPVALLH V*NEVCLKWQSQMQFGESDLSP*ISFDKQQGGVI LIYLKKTSFS*VI*MCTMYTLVLRVSV*FFFNK LLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDY RGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWN EPSINFYKRRGASDLSSEEGWRLFKIDKEYLLF* NKFPTSLAFYAVCSEIIE*APIPKLYYQWRCCM FEMRSV*SGNLRCSLESQIFLLEYLSINNKVV* S*YI*KKLHSRESFKCVQCTHWYLEFLFDSFLIN YSLI*LFGVS

Sequence SAT.dAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SSWLNMNTWKNK* S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLRK DTALLVLPCTILPMTRGLASYCILRTSS**VIIE ALA*DQKF*RI*ARLQ*GVAAAACTSW*QNGMN HPSTSIKEEVLLICPVKRVGDCSRSTRSTC*KW QQRSEECCCR*QPPFYFRINSQLLLLSMLFVVK* *NEHPFQSFITSGVVACLK*GLFKVAISDAVWR VRSFSLNIFR*TTRWCDLNIFEKNFILVSHLNV YNVHTGT*SFCLILF**TTL*FNCLV*A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQAG*I*IHGRTSN LN*KRSARRWFWRAPLLPLPGCRSAERALDSGR TQHCWFCHVLFYL*PVDWQVIVS*GLLRDE*L* RLWHRIRNSEESKPGCNEVSLQQHALLGSRME*T IHQLL*KKRCF*SVQ*RGLETVQDRQGVLAKNG NRGVRSAAVDDNLHSILE*IPNFSCFLCCL**N NRMSTHSKALLPVALLHV*NEVCLKWQSQMQFGE SDLSP*ISFDKQQGGVILIYLKKTSFS*VI*MC TMYTLVLRVSV*FFFNKLLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKLAKYEYMEEQV ILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEG HSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYR GFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNE PSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMA TEE*GVLL*MTTSILF*NKFPTSLAFYAVCSEI IE*APIPKLYYQWRCCMFEMRSV*SGNLRCSLE SQIFLLEYLSINNKVV*S*YI*KKLHSRESFKCV QCTHWYLEFLFDSFLINYSLI*LFGVS

Sequence SAT.cAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KDTALLVLPCTILPMTRGLASYCILRTSS**VII EALA*DQKF*RI*ARLQ*GVAAAACTSW*QNGM NHPSTSIKEEVLLICPVKRVGDCSRSTRSTC*K WQQRSEECCCR*QPPFYFRINSQLLLLSMLFVVK **NEHPFQSFITSGVVACLK*GLFKVAISDAVW RVRSFSLNIFR*TTRWCDLNIFEKNFILVSHLN VYNVHTGT*SFCLILF**TTL*FNCLV*A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG RTQHCWFCHVLFYL*PVDWQVIVS*GLLRDE*L *RLWHRIRNSEESKPGCNEVSLQQHALLGSRME* TIHQLL*KKRCF*SVQ*RGLETVQDRQGVLAKN GNRGVRSAAVDDNLHSILE*IPNFSCFLCCL** NNRMSTHSKALLPVALLHV*NEVCLKWQSQMQFG ESDLSP*ISFDKQQGGVILIYLKKTSFS*VI*M CTMYTLVLRVSV*FFFNKLLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDY RGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWN EPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKM ATEE*GVLL*MTTSILF*NKFPTSLAFYAVCSE IIE*APIPKLYYQWRCCMFEMRSV*SGNLRCSL ESQIFLLEYLSINNKVV*S*YI*KKLHSRESFKC VQCTHWYLEFLFDSFLINYSLI*LFGVS

Sequence SAT.qAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KVTVSSFAMYMALPCTWMGGEVTKRSFTQ*SR*S **MRTQHCWFCHVLFYL*PVDWQVIVS*GLLRD E*L*RLWHRIRNSEESKPGCNEVSLQQHALLGS RME*TIHQLL*KKRCF*SVQ*RGLETVQDRQGVL AILE*IPNFSCFLCCL**NNRMSTHSKALLPVA LLHV*NEVCLKWQSQMQFGESDLSP*ISFDKQQ GGVILIYLKKTSFS*VI*MCTMYTLVLRVSV*FF FNKLLFDLIVWCK

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG RLQSLASPCTWPFRVHGWAGR*LKDPLHNKVDD HDK*GHSIVGFAMYYFTYDPWIGKLLYLEDFFVM SDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVA EWNEPSINFYKRRGASDLSSEEGWRLFKIDKEY LLF*NKFPTSLAFYAVCSEIIE*APIPKLYYQWR CCMFEMRSV*SGNLRCSLESQIFLLEYLSINNK VV*S*YI*KKLHSRESFKCVQCTHWYLEFLFDS FLINYSLI*LFGVS

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GYSL*LRHVHGPSVYMDGRGGN*KILYTIK*MI MINEDTALLVLPCTILPMTRGLASYCILRTSS* *VIIEALA*DQKF*RI*ARLQ*GVAAAACTSW*Q NGMNHPSTSIKEEVLLICPVKRVGDCSRSTRST CYFRINSQLLLLSMLFVVK**NEHPFQSFITSG VVACLK*GLFKVAISDAVWRVRSFSLNIFR*TTR WCDLNIFEKNFILVSHLNVYNVHTGT*SFCLIL F**TTL*FNCLV*A

Sequence SAT.oAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KVTVSSFAMYMALPCTWMGGEVTKRSFTQ*SR*S **MRTQHCWFCHVLFYL*PVDWQVIVS*GLLRD E*L*RLWHRIRNSEESKPGCNEVSLQQHALLGS RME*TIHQLL*KKRCF*SVQ*RGLETVQDRQGVL AKNGNRGVRSAAVDDNLHSILE*IPNFSCFLCC L**NNRMSTHSKALLPVALLHV*NEVCLKWQSQ MQFGESDLSP*ISFDKQQGGVILIYLKKTSFS*V I*MCTMYTLVLRVSV*FFFNKLLFDLIVWCK

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG RLQSLASPCTWPFRVHGWAGR*LKDPLHNKVDD HDK*GHSIVGFAMYYFTYDPWIGKLLYLEDFFVM SDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVA EWNEPSINFYKRRGASDLSSEEGWRLFKIDKEY LLKMATEE*GVLL*MTTSILF*NKFPTSLAFYAV CSEIIE*APIPKLYYQWRCCMFEMRSV*SGNLR CSLESQIFLLEYLSINNKVV*S*YI*KKLHSRE SFKCVQCTHWYLEFLFDSFLINYSLI*LFGVS

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GYSL*LRHVHGPSVYMDGRGGN*KILYTIK*MI MINEDTALLVLPCTILPMTRGLASYCILRTSS* *VIIEALA*DQKF*RI*ARLQ*GVAAAACTSW*Q NGMNHPSTSIKEEVLLICPVKRVGDCSRSTRST C*KWQQRSEECCCR*QPPFYFRINSQLLLLSML FVVK**NEHPFQSFITSGVVACLK*GLFKVAISD AVWRVRSFSLNIFR*TTRWCDLNIFEKNFILVS HLNVYNVHTGT*SFCLILF**TTL*FNCLV*A

Sequence SAT.jAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KVTPRPFQKPERPSVMTQHCWFCHVLFYL*PVDW QVIVS*GLLRDE*L*RLWHRIRNSEESKPGCNE VSLQQHALLGSRME*TIHQLL*KKRCF*SVQ*R GLETVQDRQGVLAKNGNRGVRSAAVDDNLHSILE *IPNFSCFLCCL**NNRMSTHSKALLPVALLHV *NEVCLKWQSQMQFGESDLSP*ISFDKQQGGVI LIYLKKTSFS*VI*MCTMYTLVLRVSV*FFFNKL LFDLIVWCK

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG R*PLALSRSQRDQVL*HSIVGFAMYYFTYDPWI GKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMR CRCSSMHFLVAEWNEPSINFYKRRGASDLSSEE GWRLFKIDKEYLLKMATEE*GVLL*MTTSILF* NKFPTSLAFYAVCSEIIE*APIPKLYYQWRCCMF EMRSV*SGNLRCSLESQIFLLEYLSINNKVV*S *YI*KKLHSRESFKCVQCTHWYLEFLFDSFLIN YSLI*LFGVS

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GNPSPFPEARETKCYDTALLVLPCTILPMTRGL ASYCILRTSS**VIIEALA*DQKF*RI*ARLQ* GVAAAACTSW*QNGMNHPSTSIKEEVLLICPVKR VGDCSRSTRSTC*KWQQRSEECCCR*QPPFYFR INSQLLLLSMLFVVK**NEHPFQSFITSGVVAC LK*GLFKVAISDAVWRVRSFSLNIFR*TTRWCDL NIFEKNFILVSHLNVYNVHTGT*SFCLILF**T TL*FNCLV*A

Sequence SAT.hAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

Frame 1

Frame 2

Sequence SAT.aAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGA AGGTAACCCCTCGCCCTTTCCAGAAGCCAGAGAGACCAAGTGTTATGTAAGAAGTAGTGTCGGCTGTGTAGAACCACTGACTACACAGGCCGAAGTTACT GAGAACTTGGACAGAAAAAATAGCCAGCAAGTGTTCAAACTACTGAGGAAAAAAAAAAATTAGATATGCTGCACTTAAGAATACTAGGGCAGGTTAAAAG AGCTGTTTAAGTAAGTATCAGAGTGCTGTGGAGACTCGGAAGTGTTTAAGCTGCTTAAGTAAGTATAAGTGCTGTGGAGACCCGGAAGAGTTAGATATAA TGTCATTTGTTGTAATTCAGTTTCATAAAATGGTTCTTGTTTGACCCTAACGTAACAGTTTTTGTAATTGTGTTAAATCACATTTTTTTCTTTAATTTGT CCCAATCTTCAGGTTACAGTCTCTAGCTTCGCCATGTACATGGCCCTTCCGTGTACATGGATGGGCGGGGAGGTAACTAAAAGATCCTTTACACAATAAA GTAGATGATCATGATAAATGAGGTAAGGTCCTATTATCACACACTTCAAACACGGTAGATCAGAAACCCACTATGATACTCGCTTCCTGTCTGTTTGCTA AGGAATATAAAATGGCTAGAAAGTTTAATTTGAAACCTTTGCCTCCATTTGGAATAGTAGACACCAGTTAAGAGGGTGTCAGATGCCTTTTTTTTTTTTT TTTTTTTGGCTGGTCCCTGTTGATTGGTCAGAAGACAGCTCAGCTAAAAGGGGAAGTTGTCTGGGTGGTTGCTTTTTTTCTGACGTCTGTTCCTCAGGCT GGAAGAAATGAGCAGAAAACAAGGGATGAGTACTTTTTAGAGTATGTGCATGTTACGTAATACCTGTTTCTGGGCAATGCTGCTTCTTCTGACTCAACAA ATGGGGAGAGCAAATTGATGTTCTGACCTTTAAGGAAGCAAGAGTTTGTAAACTTCCAAATATTTACTATTCTGAACTGCCGTGTAAACCTGACGTATTC CCAAGTCAACATACCAGTATACCAATAGGATGTGAATAATGTGTGTGTTGAGTTTAAAACCATAGCAGTTTTGCTCTGGCAAGTAATGAAAGCGTTCTCG CTTCCTGAGTGTGAGCTCCAGCAGACTGCAGAGTGGCCAGTCCACAGTTGTAGCCTGACTTCAGTGAGTTCTGATGTGTGCTTTTTGCAAATACATGTTC TCAGAACAGTGAGATCATCCAGCAGTGGCCTGGACTGCACTCACATAAAAATCATGAGACAGCCATGGCTACTTGTTTCTGTAATACATGCATGTGTGTT TTTTAAAACCTATGATAGGCCTCTGATTCTGCAGCTGCAACTTTTATGGAATGTTTTCCTTCTCCACATCTCATGTGATGCTCTTATTACAGGACACAGC ATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTATAGAGGCTTTG GCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAACCATCCAT CAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTAAAAATGGCAACAGAG GAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATAATAGAAT GAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGAT CTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGT ACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KVTPRPFQKPERPSVM*EVVSAV*NH*LHRPKLL RTWTEKIASKCSNY*GKKKIRYAALKNTRAG*K SCLSKYQSAVETRKCLSCLSKYKCCGDPEELDI MSFVVIQFHKMVLV*P*RNSFCNCVKSHFFL*FV PIFRLQSLASPCTWPFRVHGWAGR*LKDPLHNK VDDHDK*GKVLLSHTSNTVDQKPTMILASCLFA KEYKMARKFNLKPLPPFGIVDTS*EGVRCLFFFF FFGWSLLIGQKTAQLKGEVVWVVAFFLTSVPQA GRNEQKTRDEYFLEYVHVT*YLFLGNAASSDST NGESKLMF*PLRKQEFVNFQIFTILNCRVNLTYS QVNIPVYQ*DVNNVCVEFKTIAVLLWQVMKAFS LPECELQQTAEWPVHSCSLTSVSSDVCFLQIHV LRTVRSSSSGLDCTHIKIMRQPWLLVSVIHACVF FKTYDRPLILQLQLLWNVFLLHISCDALITGHS IVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGF GIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSI NFYKRRGASDLSSEEGWRLFKIDKEYLLKMATE E*GVLL*MTTSILF*NKFPTSLAFYAVCSEIIE *APIPKLYYQWRCCMFEMRSV*SGNLRCSLESQI FLLEYLSINNKVV*S*YI*KKLHSRESFKCVQC THWYLEFLFDSFLINYSLI*LFGVS

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG R*PLALSRSQRDQVLCKK*CRLCRTTDYTGRSY *ELGQKK*PASVQTTEEKKKLDMLHLRILGQVKR AV*VSIRVLWRLGSV*AA*VSISAVETRKS*I* CHLL*FSFIKWFLFDPNVTVFVIVLNHIFFFNL SQSSGYSL*LRHVHGPSVYMDGRGGN*KILYTIK *MIMINEVRSYYHTLQTR*IRNPL*YSLPVCLL RNIKWLESLI*NLCLHLE**TPVKRVSDAFFFF FFLAGPC*LVRRQLS*KGKLSGWLLFF*RLFLRL EEMSRKQGMSTF*SMCMLRNTCFWAMLLLLTQQ MGRAN*CSDL*GSKSL*TSKYLLF*TAV*T*RI PKSTYQYTNRM*IMCVLSLKP*QFCSGK**KRSR FLSVSSSRLQSGQSTVVA*LQ*VLMCAFCKYMF SEQ*DHPAVAWTALT*KS*DSHGYLFL*YMHVC FLKPMIGL*FCSCNFYGMFSFSTSHVMLLLQDTA LLVLPCTILPMTRGLASYCILRTSS**VIIEAL A*DQKF*RI*ARLQ*GVAAAACTSW*QNGMNHP STSIKEEVLLICPVKRVGDCSRSTRSTC*KWQQR SEECCCR*QPPFYFRINSQLLLLSMLFVVK**N EHPFQSFITSGVVACLK*GLFKVAISDAVWRVR SFSLNIFR*TTRWCDLNIFEKNFILVSHLNVYNV HTGT*SFCLILF**TTL*FNCLV*A

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GNPSPFPEARETKCYVRSSVGCVEPLTTQAEVT ENLDRKNSQQVFKLLRKKKN*ICCT*EY*GRLK ELFK*VSECCGDSEVFKLLK*V*VLWRPGRVRYN VICCNSVS*NGSCLTLT*QFL*LC*ITFFSLIC PNLQVTVSSFAMYMALPCTWMGGEVTKRSFTQ* SR*S**MR*GPIITHFKHGRSETHYDTRFLSVC* GI*NG*KV*FETFASIWNSRHQLRGCQMPFFFF FFWLVPVDWSEDSSAKRGSCLGGCFFSDVCSSG WKK*AENKG*VLFRVCACYVIPVSGQCCFF*LNK WGEQIDVLTFKEARVCKLPNIYYSELPCKPDVF PSQHTSIPIGCE*CVC*V*NHSSFALASNESVL AS*V*APADCRVASPQL*PDFSEF*CVLFANTCS QNSEIIQQWPGLHSHKNHETAMATCFCNTCMCV F*NL**ASDSAAATFMECFPSPHLM*CSYYRTQ HCWFCHVLFYL*PVDWQVIVS*GLLRDE*L*RLW HRIRNSEESKPGCNEVSLQQHALLGSRME*TIH QLL*KKRCF*SVQ*RGLETVQDRQGVLAKNGNR GVRSAAVDDNLHSILE*IPNFSCFLCCL**NNRM STHSKALLPVALLHV*NEVCLKWQSQMQFGESD LSP*ISFDKQQGGVILIYLKKTSFS*VI*MCTM YTLVLRVSV*FFFNKLLFDLIVWCK

Sequence SAT.bAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGAAAAAGGTAATTCAACAGTGGCGGGACGGGGGACAAGCGTTCGGTGCCTGTCACCTTCGCCAAGGCCATTACCGCCCCTGCTCCCCCT TCTTGCAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGAAGGTAACCCC TCGCCCTTTCCAGAAGCCAGAGAGACCAAGTGTTATGTAAGAAGTAGTGTCGGCTGTGTAGAACCACTGACTACACAGGCCGAAGTTACTGAGAACTTGG ACAGAAAAAATAGCCAGCAAGTGTTCAAACTACTGAGGAAAAAAAAAAATTAGATATGCTGCACTTAAGAATACTAGGGCAGGTTAAAAGAGCTGTTTAA GTAAGTATCAGAGTGCTGTGGAGACTCGGAAGTGTTTAAGCTGCTTAAGTAAGTATAAGTGCTGTGGAGACCCGGAAGAGTTAGATATAATGTCATTTGT TGTAATTCAGTTTCATAAAATGGTTCTTGTTTGACCCTAACGTAACAGTTTTTGTAATTGTGTTAAATCACATTTTTTTCTTTAATTTGTCCCAATCTTC AGGTTACAGTCTCTAGCTTCGCCATGTACATGGCCCTTCCGTGTACATGGATGGGCGGGGAGGTAACTAAAAGATCCTTTACACAATAAAGTAGATGATC ATGATAAATGAGGTAAGGTCCTATTATCACACACTTCAAACACGGTAGATCAGAAACCCACTATGATACTCGCTTCCTGTCTGTTTGCTAAGGAATATAA AATGGCTAGAAAGTTTAATTTGAAACCTTTGCCTCCATTTGGAATAGTAGACACCAGTTAAGAGGGTGTCAGATGCCTTTTTTTTTTTTTTTTTTTTGGC TGGTCCCTGTTGATTGGTCAGAAGACAGCTCAGCTAAAAGGGGAAGTTGTCTGGGTGGTTGCTTTTTTTCTGACGTCTGTTCCTCAGGCTGGAAGAAATG AGCAGAAAACAAGGGATGAGTACTTTTTAGAGTATGTGCATGTTACGTAATACCTGTTTCTGGGCAATGCTGCTTCTTCTGACTCAACAAATGGGGAGAG CAAATTGAAAATGCGTAAATTGGAAGGCAAGTTCTGAAATTAAACGTTGTACTTTGGCCTGATGTTCTGACCTTTAAGGAAGCAAGAGTTTGTAAACTTC CAAATATTTACTATTCTGAACTGCCGTGTAAACCTGACGTATTCCCAAGTCAACATACCAGTATACCAATAGGATGTGAATAATGTGTGTGTTGAGTTTA AAACCATAGCAGTTTTGCTCTGGCAAGTAATGAAAGCGTTCTCGCTTCCTGAGTGTGAGCTCCAGCAGACTGCAGAGTGGCCAGTCCACAGTTGTAGCCT GACTTCAGTGAGTTCTGATGTGTGCTTTTTGCAAATACATGTTCTCAGAACAGTGAGATCATCCAGCAGTGGCCTGGACTGCACTCACATAAAAATCATG AGACAGCCATGGCTACTTGTTTCTGTAATACATGCATGTGTGTTTTTTAAAACCTATGATAGGCCTCTGATTCTGCAGCTGCAACTTTTATGGAATGTTT TCCTTCTCCACATCTCATGTGATGCTCTTATTACAGGACACAGCATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATT GTATCTTGAGGACTTCTTCGTGATGAGTGATTATAGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGC AGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAACCATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGAC TGTTCAAGATCGACAAGGAGTACTTGCTAAAAATGGCAACAGAGGAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCC AACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATAATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGG TCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTT GAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGAT TTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Intron retention

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKVIQQWRDGGQAFGACHLRQGHYRPCSP FLQIC*KMVLESTPFTTAWLQKCRKSTGLRKVTP RPFQKPERPSVM*EVVSAV*NH*LHRPKLLRTW TEKIASKCSNY*GKKKIRYAALKNTRAG*KSCL SKYQSAVETRKCLSCLSKYKCCGDPEELDIMSFV VIQFHKMVLV*P*RNSFCNCVKSHFFL*FVPIF RLQSLASPCTWPFRVHGWAGR*LKDPLHNKVDD HDK*GKVLLSHTSNTVDQKPTMILASCLFAKEYK MARKFNLKPLPPFGIVDTS*EGVRCLFFFFFFG WSLLIGQKTAQLKGEVVWVVAFFLTSVPQAGRN EQKTRDEYFLEYVHVT*YLFLGNAASSDSTNGES KLKMRKLEGKF*N*TLYFGLMF*PLRKQEFVNF QIFTILNCRVNLTYSQVNIPVYQ*DVNNVCVEF KTIAVLLWQVMKAFSLPECELQQTAEWPVHSCSL TSVSSDVCFLQIHVLRTVRSSSSGLDCTHIKIM RQPWLLVSVIHACVFFKTYDRPLILQLQLLWNV FLLHISCDALITGHSIVGFAMYYFTYDPWIGKLL YLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRC SSMHFLVAEWNEPSINFYKRRGASDLSSEEGWR LFKIDKEYLLKMATEE*GVLL*MTTSILF*NKFP TSLAFYAVCSEIIE*APIPKLYYQWRCCMFEMR SV*SGNLRCSLESQIFLLEYLSINNKVV*S*YI *KKLHSRESFKCVQCTHWYLEFLFDSFLINYSLI *LFGVS

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KR*FNSGGTGDKRSVPVTFAKAITAPAPP SCRSARRWFWRAPLLPLPGCRSAERALDSGR*P LALSRSQRDQVLCKK*CRLCRTTDYTGRSY*ELG QKK*PASVQTTEEKKKLDMLHLRILGQVKRAV* VSIRVLWRLGSV*AA*VSISAVETRKS*I*CHL L*FSFIKWFLFDPNVTVFVIVLNHIFFFNLSQSS GYSL*LRHVHGPSVYMDGRGGN*KILYTIK*MI MINEVRSYYHTLQTR*IRNPL*YSLPVCLLRNI KWLESLI*NLCLHLE**TPVKRVSDAFFFFFFLA GPC*LVRRQLS*KGKLSGWLLFF*RLFLRLEEM SRKQGMSTF*SMCMLRNTCFWAMLLLLTQQMGR AN*KCVNWKASSEIKRCTLA*CSDL*GSKSL*TS KYLLF*TAV*T*RIPKSTYQYTNRM*IMCVLSL KP*QFCSGK**KRSRFLSVSSSRLQSGQSTVVA *LQ*VLMCAFCKYMFSEQ*DHPAVAWTALT*KS* DSHGYLFL*YMHVCFLKPMIGL*FCSCNFYGMF SFSTSHVMLLLQDTALLVLPCTILPMTRGLASY CILRTSS**VIIEALA*DQKF*RI*ARLQ*GVAA AACTSW*QNGMNHPSTSIKEEVLLICPVKRVGD CSRSTRSTC*KWQQRSEECCCR*QPPFYFRINS QLLLLSMLFVVK**NEHPFQSFITSGVVACLK*G LFKVAISDAVWRVRSFSLNIFR*TTRWCDLNIF EKNFILVSHLNVYNVHTGT*SFCLILF**TTL* FNCLV*A

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKGNSTVAGRGTSVRCLSPSPRPLPPLLPL LADLLEDGFGEHPFYHCLVAEVPKEHWTPEGNP SPFPEARETKCYVRSSVGCVEPLTTQAEVTENL DRKNSQQVFKLLRKKKN*ICCT*EY*GRLKELFK *VSECCGDSEVFKLLK*V*VLWRPGRVRYNVIC CNSVS*NGSCLTLT*QFL*LC*ITFFSLICPNL QVTVSSFAMYMALPCTWMGGEVTKRSFTQ*SR*S **MR*GPIITHFKHGRSETHYDTRFLSVC*GI* NG*KV*FETFASIWNSRHQLRGCQMPFFFFFFW LVPVDWSEDSSAKRGSCLGGCFFSDVCSSGWKK* AENKG*VLFRVCACYVIPVSGQCCFF*LNKWGE QIENA*IGRQVLKLNVVLWPDVLTFKEARVCKL PNIYYSELPCKPDVFPSQHTSIPIGCE*CVC*V* NHSSFALASNESVLAS*V*APADCRVASPQL*P DFSEF*CVLFANTCSQNSEIIQQWPGLHSHKNH ETAMATCFCNTCMCVF*NL**ASDSAAATFMECF PSPHLM*CSYYRTQHCWFCHVLFYL*PVDWQVI VS*GLLRDE*L*RLWHRIRNSEESKPGCNEVSL QQHALLGSRME*TIHQLL*KKRCF*SVQ*RGLET VQDRQGVLAKNGNRGVRSAAVDDNLHSILE*IP NFSCFLCCL**NNRMSTHSKALLPVALLHV*NE VCLKWQSQMQFGESDLSP*ISFDKQQGGVILIYL KKTSFS*VI*MCTMYTLVLRVSV*FFFNKLLFD LIVWCK