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Sequences

Sequence SAT.fAug05

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKDTALLVLPCTILPMTRGLASYCILRTS S**VIIEALA*DQKF*RI*ARLQ*GVAAAACTSW *QNGMNHPSTSIKEEVLLICPVKRVGDCSRSTR STC*KWQQRSEECCCR*QPPFYFRINSQLLLLS MLFVVK**NEHPFQSFITSGVVACLK*GLFKVAI SDAVWRVRSFSLNIFR*TTRWCDLNIFEKNFIL VSHLNVYNVHTGT*SFCLILF**TTL*FNCLV* A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRTQHCWFCHVLFYL*PVDWQVIVS*GLL RDE*L*RLWHRIRNSEESKPGCNEVSLQQHALL GSRME*TIHQLL*KKRCF*SVQ*RGLETVQDRQG VLAKNGNRGVRSAAVDDNLHSILE*IPNFSCFL CCL**NNRMSTHSKALLPVALLHV*NEVCLKWQ SQMQFGESDLSP*ISFDKQQGGVILIYLKKTSFS *VI*MCTMYTLVLRVSV*FFFNKLLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKGHSIVGFAMYYFTYDPWIGKLLYLEDFF VMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFL VAEWNEPSINFYKRRGASDLSSEEGWRLFKIDK EYLLKMATEE*GVLL*MTTSILF*NKFPTSLAFY AVCSEIIE*APIPKLYYQWRCCMFEMRSV*SGN LRCSLESQIFLLEYLSINNKVV*S*YI*KKLHS RESFKCVQCTHWYLEFLFDSFLINYSLI*LFGVS

Sequence SAT.aAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGA AGGTAACCCCTCGCCCTTTCCAGAAGCCAGAGAGACCAAGTGTTATGTAAGAAGTAGTGTCGGCTGTGTAGAACCACTGACTACACAGGCCGAAGTTACT GAGAACTTGGACAGAAAAAATAGCCAGCAAGTGTTCAAACTACTGAGGAAAAAAAAAAATTAGATATGCTGCACTTAAGAATACTAGGGCAGGTTAAAAG AGCTGTTTAAGTAAGTATCAGAGTGCTGTGGAGACTCGGAAGTGTTTAAGCTGCTTAAGTAAGTATAAGTGCTGTGGAGACCCGGAAGAGTTAGATATAA TGTCATTTGTTGTAATTCAGTTTCATAAAATGGTTCTTGTTTGACCCTAACGTAACAGTTTTTGTAATTGTGTTAAATCACATTTTTTTCTTTAATTTGT CCCAATCTTCAGGTTACAGTCTCTAGCTTCGCCATGTACATGGCCCTTCCGTGTACATGGATGGGCGGGGAGGTAACTAAAAGATCCTTTACACAATAAA GTAGATGATCATGATAAATGAGGTAAGGTCCTATTATCACACACTTCAAACACGGTAGATCAGAAACCCACTATGATACTCGCTTCCTGTCTGTTTGCTA AGGAATATAAAATGGCTAGAAAGTTTAATTTGAAACCTTTGCCTCCATTTGGAATAGTAGACACCAGTTAAGAGGGTGTCAGATGCCTTTTTTTTTTTTT TTTTTTTGGCTGGTCCCTGTTGATTGGTCAGAAGACAGCTCAGCTAAAAGGGGAAGTTGTCTGGGTGGTTGCTTTTTTTCTGACGTCTGTTCCTCAGGCT GGAAGAAATGAGCAGAAAACAAGGGATGAGTACTTTTTAGAGTATGTGCATGTTACGTAATACCTGTTTCTGGGCAATGCTGCTTCTTCTGACTCAACAA ATGGGGAGAGCAAATTGATGTTCTGACCTTTAAGGAAGCAAGAGTTTGTAAACTTCCAAATATTTACTATTCTGAACTGCCGTGTAAACCTGACGTATTC CCAAGTCAACATACCAGTATACCAATAGGATGTGAATAATGTGTGTGTTGAGTTTAAAACCATAGCAGTTTTGCTCTGGCAAGTAATGAAAGCGTTCTCG CTTCCTGAGTGTGAGCTCCAGCAGACTGCAGAGTGGCCAGTCCACAGTTGTAGCCTGACTTCAGTGAGTTCTGATGTGTGCTTTTTGCAAATACATGTTC TCAGAACAGTGAGATCATCCAGCAGTGGCCTGGACTGCACTCACATAAAAATCATGAGACAGCCATGGCTACTTGTTTCTGTAATACATGCATGTGTGTT TTTTAAAACCTATGATAGGCCTCTGATTCTGCAGCTGCAACTTTTATGGAATGTTTTCCTTCTCCACATCTCATGTGATGCTCTTATTACAGGACACAGC ATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTATAGAGGCTTTG GCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAACCATCCAT CAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTAAAAATGGCAACAGAG GAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATAATAGAAT GAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGAT CTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGT ACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KVTPRPFQKPERPSVM*EVVSAV*NH*LHRPKLL RTWTEKIASKCSNY*GKKKIRYAALKNTRAG*K SCLSKYQSAVETRKCLSCLSKYKCCGDPEELDI MSFVVIQFHKMVLV*P*RNSFCNCVKSHFFL*FV PIFRLQSLASPCTWPFRVHGWAGR*LKDPLHNK VDDHDK*GKVLLSHTSNTVDQKPTMILASCLFA KEYKMARKFNLKPLPPFGIVDTS*EGVRCLFFFF FFGWSLLIGQKTAQLKGEVVWVVAFFLTSVPQA GRNEQKTRDEYFLEYVHVT*YLFLGNAASSDST NGESKLMF*PLRKQEFVNFQIFTILNCRVNLTYS QVNIPVYQ*DVNNVCVEFKTIAVLLWQVMKAFS LPECELQQTAEWPVHSCSLTSVSSDVCFLQIHV LRTVRSSSSGLDCTHIKIMRQPWLLVSVIHACVF FKTYDRPLILQLQLLWNVFLLHISCDALITGHS IVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGF GIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSI NFYKRRGASDLSSEEGWRLFKIDKEYLLKMATE E*GVLL*MTTSILF*NKFPTSLAFYAVCSEIIE *APIPKLYYQWRCCMFEMRSV*SGNLRCSLESQI FLLEYLSINNKVV*S*YI*KKLHSRESFKCVQC THWYLEFLFDSFLINYSLI*LFGVS

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG R*PLALSRSQRDQVLCKK*CRLCRTTDYTGRSY *ELGQKK*PASVQTTEEKKKLDMLHLRILGQVKR AV*VSIRVLWRLGSV*AA*VSISAVETRKS*I* CHLL*FSFIKWFLFDPNVTVFVIVLNHIFFFNL SQSSGYSL*LRHVHGPSVYMDGRGGN*KILYTIK *MIMINEVRSYYHTLQTR*IRNPL*YSLPVCLL RNIKWLESLI*NLCLHLE**TPVKRVSDAFFFF FFLAGPC*LVRRQLS*KGKLSGWLLFF*RLFLRL EEMSRKQGMSTF*SMCMLRNTCFWAMLLLLTQQ MGRAN*CSDL*GSKSL*TSKYLLF*TAV*T*RI PKSTYQYTNRM*IMCVLSLKP*QFCSGK**KRSR FLSVSSSRLQSGQSTVVA*LQ*VLMCAFCKYMF SEQ*DHPAVAWTALT*KS*DSHGYLFL*YMHVC FLKPMIGL*FCSCNFYGMFSFSTSHVMLLLQDTA LLVLPCTILPMTRGLASYCILRTSS**VIIEAL A*DQKF*RI*ARLQ*GVAAAACTSW*QNGMNHP STSIKEEVLLICPVKRVGDCSRSTRSTC*KWQQR SEECCCR*QPPFYFRINSQLLLLSMLFVVK**N EHPFQSFITSGVVACLK*GLFKVAISDAVWRVR SFSLNIFR*TTRWCDLNIFEKNFILVSHLNVYNV HTGT*SFCLILF**TTL*FNCLV*A

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GNPSPFPEARETKCYVRSSVGCVEPLTTQAEVT ENLDRKNSQQVFKLLRKKKN*ICCT*EY*GRLK ELFK*VSECCGDSEVFKLLK*V*VLWRPGRVRYN VICCNSVS*NGSCLTLT*QFL*LC*ITFFSLIC PNLQVTVSSFAMYMALPCTWMGGEVTKRSFTQ* SR*S**MR*GPIITHFKHGRSETHYDTRFLSVC* GI*NG*KV*FETFASIWNSRHQLRGCQMPFFFF FFWLVPVDWSEDSSAKRGSCLGGCFFSDVCSSG WKK*AENKG*VLFRVCACYVIPVSGQCCFF*LNK WGEQIDVLTFKEARVCKLPNIYYSELPCKPDVF PSQHTSIPIGCE*CVC*V*NHSSFALASNESVL AS*V*APADCRVASPQL*PDFSEF*CVLFANTCS QNSEIIQQWPGLHSHKNHETAMATCFCNTCMCV F*NL**ASDSAAATFMECFPSPHLM*CSYYRTQ HCWFCHVLFYL*PVDWQVIVS*GLLRDE*L*RLW HRIRNSEESKPGCNEVSLQQHALLGSRME*TIH QLL*KKRCF*SVQ*RGLETVQDRQGVLAKNGNR GVRSAAVDDNLHSILE*IPNFSCFLCCL**NNRM STHSKALLPVALLHV*NEVCLKWQSQMQFGESD LSP*ISFDKQQGGVILIYLKKTSFS*VI*MCTM YTLVLRVSV*FFFNKLLFDLIVWCK