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Sequences

Sequence SAT.iAug05

AAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACTGAGAAGAGGACGCTCCC GGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAAATGGCTAAATTCGTGAT CCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGTAGCGGAGAGCCAGAGCTCCTCCCGGGGCGTGGGGTGGAGGTGGCTCCGT TTCCCAGAGTGGGGTGATTCACGTCTTGAGGTCTCGGCCGGTGTGAGAGCCCAGCCTGTTCCCCTTTCTGCGCCCATCCCCGGCTCGGCCGCCACCCCGC CCGCCCGCCCCATTCCGTTCCCCTGAGCTGGCCGGGCCGGAGCCTCATTTTGTTTTCTACTTTTTTCTCTCCTATGTGCATCCCCCCAGGAGCTGGCTAA ATATGAATACATGGAAGAACAAGTAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTG CCGAAAGAGCACTGGACTCCGGAAGGACACAGCATTGTTGGTTTTGCCATGTACTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCG TGATGAGTGATTATAGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGT AGCAGAATGGAATGAACCATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAG TACTTGCTAAAAATGGCAACAGAGGAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATG CTGTTTGTAGTGAAATAATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCT CAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGT GAGTCATTTAAATGTGTACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Alternative donor

Frame 0

KCAALSRGPTGVYPSLAEVPWVMVPA*LRRGRS RETNEEPPPPTVQVQGPGPQREEKQKTKMAKFVI RPATAADCSDILRLIKVAESQSSSRGVGWRWLR FPEWGDSRLEVSAGVRAQPVPLSAPIPGSAATP PARPIPFP*AGRAGASFCFLLFSLLCASPQELAK YEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEV PKEHWTPEGHSIVGFAMYYDPWIGKLLYLEDFF VMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLV AEWNEPSINFYKRRGASDLSSEEGWRLFKIDKE YLLKMATEE*GVLL*MTTSILF*NKFPTSLAFY AVCSEIIE*APIPKLYYQWRCCMFEMRSV*SGNL RCSLESQIFLLEYLSINNKVV*S*YI*KKLHSR ESFKCVQCTHWYLEFLFDSFLINYSLI*LFGVS

Frame 1

NAQLLVAGRLVFIRHSPRFLGSWCQPD*EEDAP GRRMRNHLLLLFKYRGLVRKGKKSKRRKWLNS* SAQPLPPTAVTYCG*SR*RRARAPPGAWGGGGSV SQSGVIHVLRSRPV*EPSLFPFLRPSPARPPPR PPAPFRSPELAGPEPHFVFYFFLSYVHPPRSWL NMNTWKNK*S*LKKIC*KMVLESTPFTTAWLQKC RKSTGLRKDTALLVLPCTMTRGLASYCILRTSS **VIIEALA*DQKF*RI*ARLQ*GVAAAACTSW *QNGMNHPSTSIKEEVLLICPVKRVGDCSRSTRS TC*KWQQRSEECCCR*QPPFYFRINSQLLLLSM LFVVK**NEHPFQSFITSGVVACLK*GLFKVAI SDAVWRVRSFSLNIFR*TTRWCDLNIFEKNFILV SHLNVYNVHTGT*SFCLILF**TTL*FNCLV*A

Frame 2

MRSS*SRADWCLSVTRRGSLGHGASLTEKRTLP GDE*GTTSSYCSSTGAWSAKGRKAKDENG*IRD PPSHCRRLQ*HTAADQGSGEPELLPGRGVEVAP FPRVG*FTS*GLGRCESPACSPFCAHPRLGRHPA RPPHSVPLSWPGRSLILFSTFFSPMCIPPGAG* I*IHGRTSNLN*KRSARRWFWRAPLLPLPGCRS AERALDSGRTQHCWFCHVL*PVDWQVIVS*GLLR DE*L*RLWHRIRNSEESKPGCNEVSLQQHALLG SRME*TIHQLL*KKRCF*SVQ*RGLETVQDRQG VLAKNGNRGVRSAAVDDNLHSILE*IPNFSCFLC CL**NNRMSTHSKALLPVALLHV*NEVCLKWQS QMQFGESDLSP*ISFDKQQGGVILIYLKKTSFS *VI*MCTMYTLVLRVSV*FFFNKLLFDLIVWCK

Sequence SAT.eAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGA AGGACACAGCATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTAT AGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATG AACCATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTATTTTA GAATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATAATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATG TTTGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGAT CTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAA CTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Alternative donor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KDTALLVLPCTILPMTRGLASYCILRTSS**VII EALA*DQKF*RI*ARLQ*GVAAAACTSW*QNGM NHPSTSIKEEVLLICPVKRVGDCSRSTRSTCYF RINSQLLLLSMLFVVK**NEHPFQSFITSGVVAC LK*GLFKVAISDAVWRVRSFSLNIFR*TTRWCD LNIFEKNFILVSHLNVYNVHTGT*SFCLILF** TTL*FNCLV*A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG RTQHCWFCHVLFYL*PVDWQVIVS*GLLRDE*L *RLWHRIRNSEESKPGCNEVSLQQHALLGSRME* TIHQLL*KKRCF*SVQ*RGLETVQDRQGVLAIL E*IPNFSCFLCCL**NNRMSTHSKALLPVALLH V*NEVCLKWQSQMQFGESDLSP*ISFDKQQGGVI LIYLKKTSFS*VI*MCTMYTLVLRVSV*FFFNK LLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDY RGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWN EPSINFYKRRGASDLSSEEGWRLFKIDKEYLLF* NKFPTSLAFYAVCSEIIE*APIPKLYYQWRCCM FEMRSV*SGNLRCSLESQIFLLEYLSINNKVV* S*YI*KKLHSRESFKCVQCTHWYLEFLFDSFLIN YSLI*LFGVS

Sequence SAT.dAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGCTGGCTAAATATGAATACATGGAAGAACAAGTAA TCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGAAGG ACACAGCATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTATAGA GGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAAC CATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTAAAAATGGC AACAGAGGAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATA ATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGA GTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGT ACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Alternative donor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SSWLNMNTWKNK* S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLRK DTALLVLPCTILPMTRGLASYCILRTSS**VIIE ALA*DQKF*RI*ARLQ*GVAAAACTSW*QNGMN HPSTSIKEEVLLICPVKRVGDCSRSTRSTC*KW QQRSEECCCR*QPPFYFRINSQLLLLSMLFVVK* *NEHPFQSFITSGVVACLK*GLFKVAISDAVWR VRSFSLNIFR*TTRWCDLNIFEKNFILVSHLNV YNVHTGT*SFCLILF**TTL*FNCLV*A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQAG*I*IHGRTSN LN*KRSARRWFWRAPLLPLPGCRSAERALDSGR TQHCWFCHVLFYL*PVDWQVIVS*GLLRDE*L* RLWHRIRNSEESKPGCNEVSLQQHALLGSRME*T IHQLL*KKRCF*SVQ*RGLETVQDRQGVLAKNG NRGVRSAAVDDNLHSILE*IPNFSCFLCCL**N NRMSTHSKALLPVALLHV*NEVCLKWQSQMQFGE SDLSP*ISFDKQQGGVILIYLKKTSFS*VI*MC TMYTLVLRVSV*FFFNKLLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKLAKYEYMEEQV ILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEG HSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYR GFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNE PSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMA TEE*GVLL*MTTSILF*NKFPTSLAFYAVCSEI IE*APIPKLYYQWRCCMFEMRSV*SGNLRCSLE SQIFLLEYLSINNKVV*S*YI*KKLHSRESFKCV QCTHWYLEFLFDSFLINYSLI*LFGVS

Sequence SAT.cAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGA AGGACACAGCATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTAT AGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATG AACCATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTAAAAAT GGCAACAGAGGAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAA ATAATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGG AGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATG TGTACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Alternative donor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KDTALLVLPCTILPMTRGLASYCILRTSS**VII EALA*DQKF*RI*ARLQ*GVAAAACTSW*QNGM NHPSTSIKEEVLLICPVKRVGDCSRSTRSTC*K WQQRSEECCCR*QPPFYFRINSQLLLLSMLFVVK **NEHPFQSFITSGVVACLK*GLFKVAISDAVW RVRSFSLNIFR*TTRWCDLNIFEKNFILVSHLN VYNVHTGT*SFCLILF**TTL*FNCLV*A

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG RTQHCWFCHVLFYL*PVDWQVIVS*GLLRDE*L *RLWHRIRNSEESKPGCNEVSLQQHALLGSRME* TIHQLL*KKRCF*SVQ*RGLETVQDRQGVLAKN GNRGVRSAAVDDNLHSILE*IPNFSCFLCCL** NNRMSTHSKALLPVALLHV*NEVCLKWQSQMQFG ESDLSP*ISFDKQQGGVILIYLKKTSFS*VI*M CTMYTLVLRVSV*FFFNKLLFDLIVWCK

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDY RGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWN EPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKM ATEE*GVLL*MTTSILF*NKFPTSLAFYAVCSE IIE*APIPKLYYQWRCCMFEMRSV*SGNLRCSL ESQIFLLEYLSINNKVV*S*YI*KKLHSRESFKC VQCTHWYLEFLFDSFLINYSLI*LFGVS

Sequence SAT.kAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGATGCAGAAGTGCCGAAAGAGCACTGGACTCCGGAAGGACACAGCATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGAT TGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTATAGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTATGTCTT AGTTTTTGGTTTCCAAATTTGTAAGTTTACTGGATTATTTTAATGATGGAATAAAAATTGGGTCTTGAGAGCAGGCTGAAATGTCACTGAGTGTGTGTTT TACTCTCTCATAATAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAACCATCCATCAACTTCTATAAAAGAAGA GGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTAAAAATGGCAACAGAGGAGTGAGGAGTGCTGCTGT AGATGACAACCTCCATTCTATTTTAGAATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATAATAGAATGAGCACCCATTCCAAAGCT TTATTACCAGTGGCGTTGTTGCATGTTTGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTT TCGATAAACAACAAGGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGTACACACTGGTACTTAGAGT TTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Alternative donor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LMQKCRKSTGLRKDTALLVLPCTILPMTRG LASYCILRTSS**VIIEALA*DQKF*RI*ARYVL VFGFQICKFTGLF**WNKNWVLRAG*NVTECVF YSLIIGCNEVSLQQHALLGSRME*TIHQLL*KK RCF*SVQ*RGLETVQDRQGVLAKNGNRGVRSAAV DDNLHSILE*IPNFSCFLCCL**NNRMSTHSKA LLPVALLHV*NEVCLKWQSQMQFGESDLSP*IS FDKQQGGVILIYLKKTSFS*VI*MCTMYTLVLRV SV*FFFNKLLFDL

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*CRSAERALDSGRTQHCWFCHVLFYL*PVD WQVIVS*GLLRDE*L*RLWHRIRNSEESKPGMS *FLVSKFVSLLDYFNDGIKIGS*EQAEMSLSVCF TLS**VAMRCRCSSMHFLVAEWNEPSINFYKRR GASDLSSEEGWRLFKIDKEYLLKMATEE*GVLL *MTTSILF*NKFPTSLAFYAVCSEIIE*APIPKL YYQWRCCMFEMRSV*SGNLRCSLESQIFLLEYL SINNKVV*S*YI*KKLHSRESFKCVQCTHWYLE FLFDSFLINYSLI*

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTDAEVPKEHWTPEGHSIVGFAMYYFTYDPWI GKLLYLEDFFVMSDYRGFGIGSEILKNLSQVCL SFWFPNL*VYWIILMME*KLGLESRLKCH*VCV LLSHNRLQ*GVAAAACTSW*QNGMNHPSTSIKEE VLLICPVKRVGDCSRSTRSTC*KWQQRSEECCC R*QPPFYFRINSQLLLLSMLFVVK**NEHPFQS FITSGVVACLK*GLFKVAISDAVWRVRSFSLNIF R*TTRWCDLNIFEKNFILVSHLNVYNVHTGT*S FCLILF**TTL*F

Sequence SAT.jAug05

GAGCCGGGCCAATGTTCAAATGCGCAGCTCTTAGTCGCGGGCCGACTGGTGTTTATCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACT GAGAAGAGGACGCTCCCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAAA ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAG TAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGA AGGTAACCCCTCGCCCTTTCCAGAAGCCAGAGAGACCAAGTGTTATGACACAGCATTGTTGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTG GCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTATAGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAG GTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAACCATCCATCAACTTCTATAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAG GGTTGGAGACTGTTCAAGATCGACAAGGAGTACTTGCTAAAAATGGCAACAGAGGAGTGAGGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGA ATAAATTCCCAACTTCTCTTGCTTTCTATGCTGTTTGTAGTGAAATAATAGAATGAGCACCCATTCCAAAGCTTTATTACCAGTGGCGTTGTTGCATGTT TGAAATGAGGTCTGTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATCT TAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGTACACACTGGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACT ACTCTTTGATTTAATTGTTTGGTGTAAGCA

SAT.F3 (forward primer)
SAT.R6 (reverse primer)
Alternative donor

Frame 0

EPGQCSNAQLLVAGRLVFIRHSPRFLGSWCQPD *EEDAPGRRMRNHLLLLFKYRGLVRKGKKSKRRK WLNS*SAQPLPPTAVTYCG*SRSWLNMNTWKNK *S*LKKIC*KMVLESTPFTTAWLQKCRKSTGLR KVTPRPFQKPERPSVMTQHCWFCHVLFYL*PVDW QVIVS*GLLRDE*L*RLWHRIRNSEESKPGCNE VSLQQHALLGSRME*TIHQLL*KKRCF*SVQ*R GLETVQDRQGVLAKNGNRGVRSAAVDDNLHSILE *IPNFSCFLCCL**NNRMSTHSKALLPVALLHV *NEVCLKWQSQMQFGESDLSP*ISFDKQQGGVI LIYLKKTSFS*VI*MCTMYTLVLRVSV*FFFNKL LFDLIVWCK

Frame 1

SRANVQMRSS*SRADWCLSVTRRGSLGHGASLT EKRTLPGDE*GTTSSYCSSTGAWSAKGRKAKDE NG*IRDPPSHCRRLQ*HTAADQGAG*I*IHGRTS NLN*KRSARRWFWRAPLLPLPGCRSAERALDSG R*PLALSRSQRDQVL*HSIVGFAMYYFTYDPWI GKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMR CRCSSMHFLVAEWNEPSINFYKRRGASDLSSEE GWRLFKIDKEYLLKMATEE*GVLL*MTTSILF* NKFPTSLAFYAVCSEIIE*APIPKLYYQWRCCMF EMRSV*SGNLRCSLESQIFLLEYLSINNKVV*S *YI*KKLHSRESFKCVQCTHWYLEFLFDSFLIN YSLI*LFGVS

Frame 2

AGPMFKCAALSRGPTGVYPSLAEVPWVMVPA*L RRGRSRETNEEPPPPTVQVQGPGPQREEKQKTK MAKFVIRPATAADCSDILRLIKELAKYEYMEEQ VILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPE GNPSPFPEARETKCYDTALLVLPCTILPMTRGL ASYCILRTSS**VIIEALA*DQKF*RI*ARLQ* GVAAAACTSW*QNGMNHPSTSIKEEVLLICPVKR VGDCSRSTRSTC*KWQQRSEECCCR*QPPFYFR INSQLLLLSMLFVVK**NEHPFQSFITSGVVAC LK*GLFKVAISDAVWRVRSFSLNIFR*TTRWCDL NIFEKNFILVSHLNVYNVHTGT*SFCLILF**T TL*FNCLV*A