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Sequences

Sequence TPD52.bAug05

CGGGCGGCGCCGCCGCGCCCGACGCCTCTGGGGGTGGGGACTTCGCGGGAGTCGCCGGCGGAGGCGAGGAGGAGCTCTGCGCGGCGCGGCGGGCGATCCG AGCCGGGACGGGCTGCAGGCGGGGGTGCTGCAGAGGACACGAGGCGGCGGGCTGGAGACATGGACCGCGGCGAGCAAGGTCTGCTGAGAACAGACCCAGT CCCTGAGGAAGGAGAAGATGTTGCTGCCACGATCAGTGCCACAGAGACCCTCTCGGAAGAGGAGCAGGAAGAGCTAAGAAGAGAACTTGCAAAGGTAGAA GAAGAAATCCAGACTCTGTCTCAAGTGTTAGCAGCAAAAGAGAAGCATCTAGCAGAGATCAAGCGGAAACTTGGAATCAATTCTCTACAGGAACTAAAAC AGAACATTGCCAAAGGGTGGCAAGACGTGACAGCAACATCTGCTTACAAGAAGACATCTGAAACCTTATCCCAGGCTGGACAGAAGGCCTCAGCTGCTTT TTCGTCTGTTGGCTCAGTCATCACCAAAAAGCTGGAAGATGTAAAATTGCAAGCCTTTTCACATTCCTTTAGTATACGTTCCATTCAGCATTCAATTAGC ATGCCTGCTATGAGAAACTCCCCAACTTTTAAATCATTTGAAGAAAAGGTCGAAAACTTAAAGTCTAAAGTAGGGGGAACCAAGCCTGCTGGTGGTGATT TTGGAGAAGTCTTGAATTCGGCTGCAAATGCTAGTGCCACCACCACGGAGCCTCTTCCAGAAAAGACACAGGAGAGCCTGTGAGATTCCTACCTTTGTTC TGCTACCCACTGCCAGATGCTGCAAGCGAGGTCCAAGCACATCTTGTCAACATGCATTGCCATGAATTTCTACCAGATGTGCTTTTATTTAGCTTTACAT ATTCCTTTGACCAAATAGTTTGTGGGTTAAACAAAATGAAAATATCTTCACCTCTATTCTTGGGAAACACCCTTTAGTGTACATTTATGTTCCTTTATTT AGGAAACACCATTATAAAAACACTTATAGTAAATGGGGACATTCACTATAATGATCTAAGAAGCTACAGATTGTCATAGTTGTTTTCCTGCTTTACAAAA TTGCTCCAGATCTGGAATGCCAGTTTGACCTTTGTCTTCTATAATATTTCCTTTTTTTCCCCTCTTTGAATCTCTGTATATTTGATTCTTAACTAAAATT GTTCTCTTAAATATTCTGAATCCTGGTAATTAAAAGTTTGGGTGTATTTTCTTTACCTCCAAGGAAAGAACTACTAGCTACAAAAAATATTTTGGAATAA GCATTGTTTTGGTATAAGGTACATATTTTGGTTGAAGACACCAGACTGAAGTAAACAGCTGTGCATCCAATTTATTATAGTTTTGTAAGTAACAATATGT AATCAAACTTCTAGGTGACTTGAGAGTGGAACCTCCTATATCATTATTTAGCACCGTTTGTGACAGTAACCATTTCAGTGTATTGTTTATTATACCACTT ATATCAACTTATTTTTCACCAGGTTAAAATTTTAATTTCTACAAAATAACATTCTGAATCAAGCACACTGTATGTTCAGTAGGTTGAACTATGAACACTG TCATCAATGTTCAGTTCAAAAGCCTGAAAGTTTAGATCTAGAAGCTGGTAAAAATGACAATATCAATCACATTAGGGGAACCATTGTTGTCTTCACTTAA TCCATTTAGCACTATTTAAAATAAGCACACCAAGTTATATGACTAATATAACTTGAAAATTTTTTATACTGAGGGGTTGGTGATAACTCTTGAGGATGTA ATGCATTAATAAAAATCAACTCATCATTTTCTACTTGTTTTCAATGTGTTGGAAACTGTAAAATGATACTGTAGAACCTGTCTCCTACTTTGAAAACTGA ATGTCAGGGCTGAGTGAATCAAAGTGTCTAGACATATTTGCATAGAGGCCAAGGTATTCTATTCTAATAACTGCTTACTCAACACTACCACCTTTTCCTT ATACTGTATATGATTATGGCCTACAATGTTGTATTTGTTATTTATTAAATTGTGATTGTTTTATTATTGTTTATGCCAAATGTTAACTGCCAAGCTTGGA GTGACCTAAAGCATTTTTTAAAAGCATGGCTAGATTTACTTCAGTATAAATTATCTTATGAAAACCAAATTTTAAAAGCCACAGGTGTTGATTGTTATAA AATAACATGCTGCCATTCTTGATTGCTAGAGTTTTTGTTAGTACTTTGGATGCAATTAAAACTATGTGCTATCACATGTGAAAAGCTTAATAAATTCCAT CTATCAGTAGTATAGGTCTCAATATTTATTATGAGACCAGTGGTCTGGAAACAGCTTGTTGTACCGAATCAACTGGAGTCTATGCTTAAAAAAAAAAAAT TTTTTTTTAACCATCCTTAAATTATTGCTTAATGGTATCATATTAACATATTCTAAATAAGGGCTTTAAGGCACAGGCTGTTGAAGCATTTTCTCAGAGG AGTGGATCTGTAGAAGTCTGTCTTTCTATAGAAATATTGTGCTTACTCAAGTGTTAAATTATTTTTTCTATGAACTAGTCTACTTCTTAAAATTCAAACA TATTCTTTTGATCACATTGTTTCTTGAGCATCCTGCCCTGCTACTAACTTTTCAACAAGGCAAAATGGAGTAAAGTGGCAATTTCTTTAGATGAGTGAAA TACCCTCAAGTCTCTTTTCTGCCCAAAAAGGGAAAAGTGATAGAAATGGGGGTGGCAAGTGGGGTGAGTGGATGAAGGTGGGTATTGGGGGTGGCTGTGA AAGAAAATAATGGAGAATCACTTTTCTAGACATCTACCTATACTTAATCTAAGAAACAAAGTAATCTACTGTAAAGTACTCTGCCCCTTGAAAGAAGTAT TAAAAAGAGTGAGGATGGATTTAGAAAAAAACATGAATTTAGAAATATTCAAAATGGTTTTTGTGGCAGATTCAATATTATGAATTCACAGATATTTAAA GAATGAGAAACATAGTAATTAGTAGAAATGCCAGAAACAGTTCCTGGTTCCTCTTGTGTTTGACACTAAGAAAATAGCAAGAGTGTGAAATCTCAGATAC TTATGAAATCTCACAGATGTAAGGACTCAAGTGTAGAAGAAAATATCCCCTTCTTACAAAAAGAAATGTCAATTTATGGAGTTTGTGGGAAATAGGGCAA GAATTCTTATGCTTATGAGAGCCAAGTAGTCAGTGGAAGAGAGTAGAGCTCAAAACTGGATTATCACCTTAGCAACTTAGAATAGTTTGAAATAGAAAAA AAGTATTTAATTTGGATCTGGATCTGTTAAGATATGCACAGTCTATTTTTTGTATAGTATTGGAAAATAAAAATGCTATAATTTGGGTATGGGTTCTTTT GTATACATTACCTGCCCATTGAGGAAAGGGGCAAGTCCATTATGCAACTTCTCTCCAAACCCTTCATATTCTGGATATGATACTTAAAGAGCCATGAGCA GCTACATTCTGCCTATCAGAAGGCATTTATTAAATGACTGCTTGCTGAAGATGTGTCCAAAGAGATCCTACATTAAGGTCATTTGTCAGAATGATGTTTG TGTTTGTTTAGAGTTGGCTGACCTCCAACTCCTGGGGTCAAGGAATCCTACTGCCTTAGCTTCCCAAATAGCTAGGACTATAGGCATGCACCCGGCCATG TGTTTTATTTATAGCTCTTAAAGCCCAGATGAAGAAATCACATTTTTGCCCATAGTGAAGAAACATTTGGCCATTGATTAGTCCTTATTTTCAGTGACTG TCCTGTTTTCATTAGATTAGAGAGACCCTGTGTGGGCCACAGTTAATATAAACCATTATCACTTTTAAGTAAACCTGCACATCTTAGATTTCATAATTTC CTTATTGTTCTGACTCAAAATGAACTAAGAGCTTTTCACTTTTTGTTTGTAAGTTCTCAGAGAGTCGGGTCTGCAAGTGCTTTTGCCTGCCAGGATTTTG TACTCAAATAAATGCTATTTGGCAACTAAAATTCTTGCCAGCTCTCATGTAATTTTGATGTTACTGAGGTTGGTTGGTAGTTTATTTTATTAATTTTGTG GTTCTTCTATCATATAGAAAAAGCCAAATACAGTTTTGTTTGAATATTTGT

TPD52.F12 (forward primer)
TPD52.R11 (reverse primer)
Alternative donor

Frame 0

RAAPPRPTPLGVGTSRESPAEARRSSARRGGRS EPGRAAGGGAAEDTRRRAGDMDRGEQGLLRTDPV PEEGEDVAATISATETLSEEEQEELRRELAKVE EEIQTLSQVLAAKEKHLAEIKRKLGINSLQELK QNIAKGWQDVTATSAYKKTSETLSQAGQKASAAF SSVGSVITKKLEDVKLQAFSHSFSIRSIQHSIS MPAMRNSPTFKSFEEKVENLKSKVGGTKPAGGD FGEVLNSAANASATTTEPLPEKTQESL*DSYLCS ATHCQMLQARSKHILSTCIAMNFYQMCFYLALH IPLTK*FVG*TK*KYLHLYSWETPFSVHLCSFI *ETPL*KHL**MGTFTIMI*EATDCHSCFPALQN CSRSGMPV*PLSSIIFPFFPLFESLYI*FLTKI VLLNILNPGN*KFGCIFFTSKERTTSYKKYFGI SIVLV*GTYFG*RHQTEVNSCASNLL*FCK*QYV IKLLGDLRVEPPISLFSTVCDSNHFSVLFIIPL ISTYFSPG*NFNFYKITF*IKHTVCSVG*TMNT VINVQFKSLKV*I*KLVKMTISITLGEPLLSSLN PFSTI*NKHTKLYD*YNLKIFYTEGLVITLEDV MH**KSTHHFLLVFNVLETVK*YCRTCLLL*KL NVRAE*IKVSRHICIEAKVFYSNNCLLNTTTFSL YCI*LWPTMLYLLFIKL*LFYYCLCQMLTAKLG VT*SIF*KHG*IYFSINYLMKTKF*KPQVLIVI K*HAAILDC*SFC*YFGCN*NYVLSHVKSLINSI YQ*YRSQYLL*DQWSGNSLLYRINWSLCLKKKN FFLTILKLLLNGIILTYSK*GL*GTGC*SIFSE EWICRSLSFYRNIVLTQVLNYFFYELVYFLKFKH ILLITLFLEHPALLLTFQQGKME*SGNFFR*VK YPQVSFLPKKGKVIEMGVASGVSG*RWVLGVAV KENNGESLF*TSTYT*SKKQSNLL*STLPLERSI KKSEDGFRKKHEFRNIQNGFCGRFNIMNSQIFK E*ET**LVEMPETVPGSSCV*H*ENSKSVKSQI LMKSHRCKDSSVEENIPFLQKEMSIYGVCGK*GK NSYAYESQVVSGRE*SSKLDYHLSNLE*FEIEK KYLIWIWIC*DMHSLFFV*YWKIKML*FGYGFF CIHYLPIEERGKSIMQLLSKPFIFWI*YLKSHEQ LHSAYQKAFIK*LLAEDVSKEILH*GHLSE*CL CLFRVG*PPTPGVKESYCLSFPNS*DYRHAPGH VFYL*LLKPR*RNHIFAHSEETFGH*LVLIFSDC PVFIRLERPCVGHS*YKPLSLLSKPAHLRFHNF LIVLTQNELRAFHFLFVSSQRVGSASAFACQDF VLK*MLFGN*NSCQLSCNFDVTEVGW*FILLILW FFYHIEKAKYSFV*IF

Frame 1

GRRRRARRLWGWGLRGSRRRRRGGALRGAAGDP SRDGLQAGVLQRTRGGGLETWTAASKVC*EQTQ SLRKEKMLLPRSVPQRPSRKRSRKS*EENLQR*K KKSRLCLKC*QQKRSI*QRSSGNLESILYRN*N RTLPKGGKT*QQHLLTRRHLKPYPRLDRRPQLL FRLLAQSSPKSWKM*NCKPFHIPLVYVPFSIQLA CLL*ETPQLLNHLKKRSKT*SLK*GEPSLLVVI LEKS*IRLQMLVPPPRSLFQKRHRRACEIPTFV LLPTARCCKRGPSTSCQHALP*ISTRCAFI*LYI FL*PNSLWVKQNENIFTSILGKHPLVYIYVPLF RKHHYKNTYSKWGHSL**SKKLQIVIVVFLLYK IAPDLECQFDLCLL*YFLFFPSLNLCIFDS*LKL FS*IF*ILVIKSLGVFSLPPRKELLATKNILE* ALFWYKVHILVEDTRLK*TAVHPIYYSFVSNNM *SNF*VT*EWNLLYHYLAPFVTVTISVYCLLYHL YQLIFHQVKILISTK*HSESSTLYVQ*VEL*TL SSMFSSKA*KFRSRSW*K*QYQSH*GNHCCLHL IHLALFKISTPSYMTNIT*KFFILRGW**LLRM* CINKNQLIIFYLFSMCWKL*NDTVEPVSYFEN* MSGLSESKCLDIFA*RPRYSILITAYSTLPPFP YTVYDYGLQCCICYLLNCDCFIIVYAKC*LPSLE *PKAFFKSMARFTSV*IIL*KPNFKSHRC*LL* NNMLPFLIARVFVSTLDAIKTMCYHM*KA**IP SISSIGLNIYYETSGLETACCTESTGVYA*KKKI FF*PSLNYCLMVSY*HILNKGFKAQAVEAFSQR SGSVEVCLSIEILCLLKC*IIFSMN*STS*NSN IFF*SHCFLSILPCY*LFNKAKWSKVAISLDE*N TLKSLFCPKREK**KWGWQVG*VDEGGYWGWL* KKIMENHFSRHLPILNLRNKVIYCKVLCPLKEV LKRVRMDLEKNMNLEIFKMVFVADSIL*IHRYLK NEKHSN**KCQKQFLVPLVFDTKKIARV*NLRY L*NLTDVRTQV*KKISPSYKKKCQFMEFVGNRA RILMLMRAK*SVEESRAQNWIITLAT*NSLK*KK SI*FGSGSVKICTVYFLYSIGK*KCYNLGMGSF VYITCPLRKGASPLCNFSPNPSYSGYDT*RAMS SYILPIRRHLLNDCLLKMCPKRSYIKVICQNDVC VCLELADLQLLGSRNPTALASQIARTIGMHPAM CFIYSS*SPDEEITFLPIVKKHLAID*SLFSVT VLFSLD*RDPVWATVNINHYHF*VNLHILDFIIS LLF*LKMN*ELFTFCL*VLRESGLQVLLPARIL YSNKCYLATKILASSHVILMLLRLVGSLFY*FC GSSII*KKPNTVLFEYL

Frame 2

GGAAAPDASGGGDFAGVAGGGEEELCAARRAIR AGTGCRRGCCRGHEAAGWRHGPRRARSAENRPS P*GRRRCCCHDQCHRDPLGRGAGRAKKRTCKGR RRNPDSVSSVSSKREASSRDQAETWNQFSTGTKT EHCQRVARRDSNICLQEDI*NLIPGWTEGLSCF FVCWLSHHQKAGRCKIASLFTFL*YTFHSAFN* HACYEKLPNF*II*RKGRKLKV*SRGNQACWW*F WRSLEFGCKC*CHHHGASSRKDTGEPVRFLPLF CYPLPDAASEVQAHLVNMHCHEFLPDVLLFSFT YSFDQIVCGLNKMKISSPLFLGNTL*CTFMFLYL GNTIIKTLIVNGDIHYNDLRSYRLS*LFSCFTK LLQIWNASLTFVFYNISFFSPL*ISVYLILN*N CSLKYSESW*LKVWVYFLYLQGKNY*LQKIFWNK HCFGIRYIFWLKTPD*SKQLCIQFIIVL*VTIC NQTSR*LESGTSYIII*HRL*Q*PFQCIVYYTT YINLFFTRLKF*FLQNNILNQAHCMFSRLNYEHC HQCSVQKPESLDLEAGKNDNINHIRGTIVVFT* SI*HYLK*AHQVI*LI*LENFLY*GVGDNS*GC NALIKINSSFSTCFQCVGNCKMIL*NLSPTLKTE CQG*VNQSV*TYLHRGQGILF**LLTQHYHLFL ILYMIMAYNVVFVIY*IVIVLLLFMPNVNCQAW SDLKHFLKAWLDLLQYKLSYENQILKATGVDCYK ITCCHS*LLEFLLVLWMQLKLCAITCEKLNKFH LSVV*VSIFIMRPVVWKQLVVPNQLESMLKKKK FFFNHP*IIA*WYHINIF*IRALRHRLLKHFLRG VDL*KSVFL*KYCAYSSVKLFFL*TSLLLKIQT YSFDHIVS*ASCPATNFSTRQNGVKWQFL*MSE IPSSLFSAQKGKSDRNGGGKWGEWMKVGIGGGCE RK*WRITFLDIYLYLI*ETK*STVKYSAP*KKY *KE*GWI*KKT*I*KYSKWFLWQIQYYEFTDI* RMRNIVISRNARNSSWFLLCLTLRK*QECEISDT YEISQM*GLKCRRKYPLLTKRNVNLWSLWEIGQ EFLCL*EPSSQWKRVELKTGLSP*QLRIV*NRK KVFNLDLDLLRYAQSIFCIVLENKNAIIWVWVLL YTLPAH*GKGQVHYATSLQTLHILDMILKEP*A ATFCLSEGIY*MTAC*RCVQRDPTLRSFVRMMF VFV*SWLTSNSWGQGILLP*LPK*LGL*ACTRPC VLFIALKAQMKKSHFCP**RNIWPLISPYFQ*L SCFH*IRETLCGPQLI*TIITFK*TCTS*IS*F PYCSDSK*TKSFSLFVCKFSESRVCKCFCLPGFC TQINAIWQLKFLPALM*F*CY*GWLVVYFINFV VLLSYRKSQIQFCLNIC

Sequence TPD52.aAug05

AGTGTCTGATGTTTGCCTGAACTGTTTGTACCTCTGGGCCATATTGCAGAACCCTGCCCTTCTTTGTTGACTGAGGAAAGCTCGCTCCCTGCCCAGGTTT TTCATTGTTGATCGAAATTAACACCAGGTGGTGAATAGAGCCCCTCCTAAGGTTGCTCAGGATAAATCATTTATTAAATAGGTCTGCTTATCAGGAGGGG CGTGAAGGCTCCCAAAAGGAAATGCTGGCACCTGGGCCCAGAAGCCAGGGCCTCTAACTCCTGGGGTTGATTTCTTCAGTGAAGTTGCACCCTACAAAGG GAATATGGCCAAAGCGGCACTCAACTGAAGGCTGATATCAGGCGATTAGACAGCCATGCATTCTGCGTTTGTCTGGAATGGATTGTAGAGAGATGGACTT ATATGAGGACTACCAGTCCCCGTTTGATTTTGATGCAGGAGTGAACAAAAGCTATCTCTACTTGTCTCCTAGTGGAAATTCATCTCCACCCGGATCACCT ACTCTTCAGAAATTTGGTCTGCTGAGAACAGACCCAGTCCCTGAGGAAGGAGAAGATGTTGCTGCCACGATCAGTGCCACAGAGACCCTCTCGGAAGAGG AGCAGGAAGAGCTAAGAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTGTTAGCAGCAAAAGAGAAGCATCTAGCAGAGATCAA GCGGAAACTTGGAATCAATTCTCTACAGGAACTAAAACAGAACATTGCCAAAGGGTGGCAAGACGTGACAGCAACATCTGCTTACAAGAAGACATCTGAA ACCTTATCCCAGGCTGGACAGAAGGCCTCAGCTGCTTTTTCGTCTGTTGGCTCAGTCATCACCAAAAAGCTGGAAGATGTAAAATTGCAAGCCTTTTCAC ATTCCTTTAGTATACGTTCCATTCAGCATTCAATTAGCATGCCTGCTATGAGAAACTCCCCAACTTTTAAATCATTTGAAGAAAAGGTCGAAAACTTAAA GTCTAAAGTAGGGGGAACCAAGCCTGCTGGTGGTGATTTTGGAGAAGTCTTGAATTCGGCTGCAAATGCTAGTGCCACCACCACGGAGCCTCTTCCAGAA AAGACACAGGAGAGCCTGTGAGATTCCTACCTTTGTTCTGCTACCCACTGCCAGATGCTGCAAGCGAGGTCCAAGCACATCTTGTCAACATGCATTGCCA TGAATTTCTACCAGATGTGCTTTTATTTAGCTTTACATATTCCTTTGACCAAATAGTTTGTGGGTTAAACAAAATGAAAATATCTTCACCTCTATTCTTG GGAAACACCCTTTAGTGTACATTTATGTTCCTTTATTTAGGAAACACCATTATAAAAACACTTATAGTAAATGGGGACATTCACTATAATGATCTAAGAA GCTACAGATTGTCATAGTTGTTTTCCTGCTTTACAAAATTGCTCCAGATCTGGAATGCCAGTTTGACCTTTGTCTTCTATAATATTTCCTTTTTTTCCCC TCTTTGAATCTCTGTATATTTGATTCTTAACTAAAATTGTTCTCTTAAATATTCTGAATCCTGGTAATTAAAAGTTTGGGTGTATTTTCTTTACCTCCAA GGAAAGAACTACTAGCTACAAAAAATATTTTGGAATAAGCATTGTTTTGGTATAAGGTACATATTTTGGTTGAAGACACCAGACTGAAGTAAACAGCTGT GCATCCAATTTATTATAGTTTTGTAAGTAACAATATGTAATCAAACTTCTAGGTGACTTGAGAGTGGAACCTCCTATATCATTATTTAGCACCGTTTGTG ACAGTAACCATTTCAGTGTATTGTTTATTATACCACTTATATCAACTTATTTTTCACCAGGTTAAAATTTTAATTTCTACAAAATAACATTCTGAATCAA GCACACTGTATGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAGTTCAAAAGCCTGAAAGTTTAGATCTAGAAGCTGGTAAAAATGACAATA TCAATCACATTAGGGGAACCATTGTTGTCTTCACTTAATCCATTTAGCACTATTTAAAATAAGCACACCAAGTTATATGACTAATATAACTTGAAAATTT TTTATACTGAGGGGTTGGTGATAACTCTTGAGGATGTAATGCATTAATAAAAATCAACTCATCATTTTCTACTTGTTTTCAATGTGTTGGAAACTGTAAA ATGATACTGTAGAACCTGTCTCCTACTTTGAAAACTGAATGTCAGGGCTGAGTGAATCAAAGTGTCTAGACATATTTGCATAGAGGCCAAGGTATTCTAT TCTAATAACTGCTTACTCAACACTACCACCTTTTCCTTATACTGTATATGATTATGGCCTACAATGTTGTATTTGTTATTTATTAAATTGTGATTGTTTT ATTATTGTTTATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCATTTTTTAAAAGCATGGCTAGATTTACTTCAGTATAAATTATCTTATGAA AACCAAATTTTAAAAGCCACAGGTGTTGATTGTTATAAAATAACATGCTGCCATTCTTGATTGCTAGAGTTTTTGTTAGTACTTTGGATGCAATTAAAAC TATGTGCTATCACATGTGAAAAGCTTAATAAATTCCATCTATCAGTAGTATAGGTCTCAATATTTATTATGAGACCAGTGGTCTGGAAACAGCTTGTTGT ACCGAATCAACTGGAGTCTATGCTTAAAAAAAAAAAATTTTTTTTTAACCATCCTTAAATTATTGCTTAATGGTATCATATTAACATATTCTAAATAAGG GCTTTAAGGCACAGGCTGTTGAAGCATTTTCTCAGAGGAGTGGATCTGTAGAAGTCTGTCTTTCTATAGAAATATTGTGCTTACTCAAGTGTTAAATTAT TTTTTCTATGAACTAGTCTACTTCTTAAAATTCAAACATATTCTTTTGATCACATTGTTTCTTGAGCATCCTGCCCTGCTACTAACTTTTCAACAAGGCA AAATGGAGTAAAGTGGCAATTTCTTTAGATGAGTGAAATACCCTCAAGTCTCTTTTCTGCCCAAAAAGGGAAAAGTGATAGAAATGGGGGTGGCAAGTGG GGTGAGTGGATGAAGGTGGGTATTGGGGGTGGCTGTGAAAGAAAATAATGGAGAATCACTTTTCTAGACATCTACCTATACTTAATCTAAGAAACAAAGT AATCTACTGTAAAGTACTCTGCCCCTTGAAAGAAGTATTAAAAAGAGTGAGGATGGATTTAGAAAAAAACATGAATTTAGAAATATTCAAAATGGTTTTT GTGGCAGATTCAATATTATGAATTCACAGATATTTAAAGAATGAGAAACATAGTAATTAGTAGAAATGCCAGAAACAGTTCCTGGTTCCTCTTGTGTTTG ACACTAAGAAAATAGCAAGAGTGTGAAATCTCAGATACTTATGAAATCTCACAGATGTAAGGACTCAAGTGTAGAAGAAAATATCCCCTTCTTACAAAAA GAAATGTCAATTTATGGAGTTTGTGGGAAATAGGGCAAGAATTCTTATGCTTATGAGAGCCAAGTAGTCAGTGGAAGAGAGTAGAGCTCAAAACTGGATT ATCACCTTAGCAACTTAGAATAGTTTGAAATAGAAAAAAAGTATTTAATTTGGATCTGGATCTGTTAAGATATGCACAGTCTATTTTTTGTATAGTATTG GAAAATAAAAATGCTATAATTTGGGTATGGGTTCTTTTGTATACATTACCTGCCCATTGAGGAAAGGGGCAAGTCCATTATGCAACTTCTCTCCAAACCC TTCATATTCTGGATATGATACTTAAAGAGCCATGAGCAGCTACATTCTGCCTATCAGAAGGCATTTATTAAATGACTGCTTGCTGAAGATGTGTCCAAAG AGATCCTACATTAAGGTCATTTGTCAGAATGATGTTTGTGTTTGTTTAGAGTTGGCTGACCTCCAACTCCTGGGGTCAAGGAATCCTACTGCCTTAGCTT CCCAAATAGCTAGGACTATAGGCATGCACCCGGCCATGTGTTTTATTTATAGCTCTTAAAGCCCAGATGAAGAAATCACATTTTTGCCCATAGTGAAGAA ACATTTGGCCATTGATTAGTCCTTATTTTCAGTGACTGTCCTGTTTTCATTAGATTAGAGAGACCCTGTGTGGGCCACAGTTAATATAAACCATTATCAC TTTTAAGTAAACCTGCACATCTTAGATTTCATAATTTCCTTATTGTTCTGACTCAAAATGAACTAAGAGCTTTTCACTTTTTGTTTGTAAGTTCTCAGAG AGTCGGGTCTGCAAGTGCTTTTGCCTGCCAGGATTTTGTACTCAAATAAATGCTATTTGGCAACTAAAATTCTTGCCAGCTCTCATGTAATTTTGATGTT ACTGAGGTTGGTTGGTAGTTTATTTTATTAATTTTGTGGTTCTTCTATCATATAGAAAAAGCCAAATACAGTTTTGTTTGAATATTTGT

TPD52.F12 (forward primer)
TPD52.R11 (reverse primer)
Alternative donor

Frame 0

SV*CLPELFVPLGHIAEPCPSLLTEESSLPAQV FHC*SKLTPGGE*SPS*GCSG*IIY*IGLLIRRG VKAPKRKCWHLGPEARASNSWG*FLQ*SCTLQR EYGQSGTQLKADIRRLDSHAFCVCLEWIVERWT YMRTTSPRLILMQE*TKAISTCLLVEIHLHPDHL LFRNLVC*EQTQSLRKEKMLLPRSVPQRPSRKR SRKS*EENLQR*KKKSRLCLKC*QQKRSI*QRS SGNLESILYRN*NRTLPKGGKT*QQHLLTRRHLK PYPRLDRRPQLLFRLLAQSSPKSWKM*NCKPFH IPLVYVPFSIQLACLL*ETPQLLNHLKKRSKT* SLK*GEPSLLVVILEKS*IRLQMLVPPPRSLFQK RHRRACEIPTFVLLPTARCCKRGPSTSCQHALP *ISTRCAFI*LYIFL*PNSLWVKQNENIFTSIL GKHPLVYIYVPLFRKHHYKNTYSKWGHSL**SKK LQIVIVVFLLYKIAPDLECQFDLCLL*YFLFFP SLNLCIFDS*LKLFS*IF*ILVIKSLGVFSLPP RKELLATKNILE*ALFWYKVHILVEDTRLK*TAV HPIYYSFVSNNM*SNF*VT*EWNLLYHYLAPFV TVTISVYCLLYHLYQLIFHQVKILISTK*HSES STLYVQ*VEL*TLSSMFSSKA*KFRSRSW*K*QY QSH*GNHCCLHLIHLALFKISTPSYMTNIT*KF FILRGW**LLRM*CINKNQLIIFYLFSMCWKL* NDTVEPVSYFEN*MSGLSESKCLDIFA*RPRYSI LITAYSTLPPFPYTVYDYGLQCCICYLLNCDCF IIVYAKC*LPSLE*PKAFFKSMARFTSV*IIL* KPNFKSHRC*LL*NNMLPFLIARVFVSTLDAIKT MCYHM*KA**IPSISSIGLNIYYETSGLETACC TESTGVYA*KKKIFF*PSLNYCLMVSY*HILNK GFKAQAVEAFSQRSGSVEVCLSIEILCLLKC*II FSMN*STS*NSNIFF*SHCFLSILPCY*LFNKA KWSKVAISLDE*NTLKSLFCPKREK**KWGWQV G*VDEGGYWGWL*KKIMENHFSRHLPILNLRNKV IYCKVLCPLKEVLKRVRMDLEKNMNLEIFKMVF VADSIL*IHRYLKNEKHSN**KCQKQFLVPLVF DTKKIARV*NLRYL*NLTDVRTQV*KKISPSYKK KCQFMEFVGNRARILMLMRAK*SVEESRAQNWI ITLAT*NSLK*KKSI*FGSGSVKICTVYFLYSI GK*KCYNLGMGSFVYITCPLRKGASPLCNFSPNP SYSGYDT*RAMSSYILPIRRHLLNDCLLKMCPK RSYIKVICQNDVCVCLELADLQLLGSRNPTALA SQIARTIGMHPAMCFIYSS*SPDEEITFLPIVKK HLAID*SLFSVTVLFSLD*RDPVWATVNINHYH F*VNLHILDFIISLLF*LKMN*ELFTFCL*VLR ESGLQVLLPARILYSNKCYLATKILASSHVILML LRLVGSLFY*FCGSSII*KKPNTVLFEYL

Frame 1

VSDVCLNCLYLWAILQNPALLC*LRKARSLPRF FIVDRN*HQVVNRAPPKVAQDKSFIK*VCLSGG A*RLPKGNAGTWAQKPGPLTPGVDFFSEVAPYKG NMAKAALN*RLISGD*TAMHSAFVWNGL*RDGL I*GLPVPV*F*CRSEQKLSLLVS*WKFISTRIT YSSEIWSAENRPSP*GRRRCCCHDQCHRDPLGRG AGRAKKRTCKGRRRNPDSVSSVSSKREASSRDQ AETWNQFSTGTKTEHCQRVARRDSNICLQEDI* NLIPGWTEGLSCFFVCWLSHHQKAGRCKIASLFT FL*YTFHSAFN*HACYEKLPNF*II*RKGRKLK V*SRGNQACWW*FWRSLEFGCKC*CHHHGASSR KDTGEPVRFLPLFCYPLPDAASEVQAHLVNMHCH EFLPDVLLFSFTYSFDQIVCGLNKMKISSPLFL GNTL*CTFMFLYLGNTIIKTLIVNGDIHYNDLR SYRLS*LFSCFTKLLQIWNASLTFVFYNISFFSP L*ISVYLILN*NCSLKYSESW*LKVWVYFLYLQ GKNY*LQKIFWNKHCFGIRYIFWLKTPD*SKQL CIQFIIVL*VTICNQTSR*LESGTSYIII*HRL* Q*PFQCIVYYTTYINLFFTRLKF*FLQNNILNQ AHCMFSRLNYEHCHQCSVQKPESLDLEAGKNDN INHIRGTIVVFT*SI*HYLK*AHQVI*LI*LENF LY*GVGDNS*GCNALIKINSSFSTCFQCVGNCK MIL*NLSPTLKTECQG*VNQSV*TYLHRGQGIL F**LLTQHYHLFLILYMIMAYNVVFVIY*IVIVL LLFMPNVNCQAWSDLKHFLKAWLDLLQYKLSYE NQILKATGVDCYKITCCHS*LLEFLLVLWMQLK LCAITCEKLNKFHLSVV*VSIFIMRPVVWKQLVV PNQLESMLKKKKFFFNHP*IIA*WYHINIF*IR ALRHRLLKHFLRGVDL*KSVFL*KYCAYSSVKL FFL*TSLLLKIQTYSFDHIVS*ASCPATNFSTRQ NGVKWQFL*MSEIPSSLFSAQKGKSDRNGGGKW GEWMKVGIGGGCERK*WRITFLDIYLYLI*ETK *STVKYSAP*KKY*KE*GWI*KKT*I*KYSKWFL WQIQYYEFTDI*RMRNIVISRNARNSSWFLLCL TLRK*QECEISDTYEISQM*GLKCRRKYPLLTK RNVNLWSLWEIGQEFLCL*EPSSQWKRVELKTGL SP*QLRIV*NRKKVFNLDLDLLRYAQSIFCIVL ENKNAIIWVWVLLYTLPAH*GKGQVHYATSLQT LHILDMILKEP*AATFCLSEGIY*MTAC*RCVQR DPTLRSFVRMMFVFV*SWLTSNSWGQGILLP*L PK*LGL*ACTRPCVLFIALKAQMKKSHFCP**R NIWPLISPYFQ*LSCFH*IRETLCGPQLI*TIIT FK*TCTS*IS*FPYCSDSK*TKSFSLFVCKFSE SRVCKCFCLPGFCTQINAIWQLKFLPALM*F*C Y*GWLVVYFINFVVLLSYRKSQIQFCLNIC

Frame 2

CLMFA*TVCTSGPYCRTLPFFVD*GKLAPCPGF SLLIEINTRW*IEPLLRLLRINHLLNRSAYQEG REGSQKEMLAPGPRSQGL*LLGLISSVKLHPTK GIWPKRHSTEG*YQAIRQPCILRLSGMDCREMDL YEDYQSPFDFDAGVNKSYLYLSPSGNSSPPGSP TLQKFGLLRTDPVPEEGEDVAATISATETLSEE EQEELRRELAKVEEEIQTLSQVLAAKEKHLAEIK RKLGINSLQELKQNIAKGWQDVTATSAYKKTSE TLSQAGQKASAAFSSVGSVITKKLEDVKLQAFS HSFSIRSIQHSISMPAMRNSPTFKSFEEKVENLK SKVGGTKPAGGDFGEVLNSAANASATTTEPLPE KTQESL*DSYLCSATHCQMLQARSKHILSTCIA MNFYQMCFYLALHIPLTK*FVG*TK*KYLHLYSW ETPFSVHLCSFI*ETPL*KHL**MGTFTIMI*E ATDCHSCFPALQNCSRSGMPV*PLSSIIFPFFP LFESLYI*FLTKIVLLNILNPGN*KFGCIFFTSK ERTTSYKKYFGISIVLV*GTYFG*RHQTEVNSC ASNLL*FCK*QYVIKLLGDLRVEPPISLFSTVC DSNHFSVLFIIPLISTYFSPG*NFNFYKITF*IK HTVCSVG*TMNTVINVQFKSLKV*I*KLVKMTI SITLGEPLLSSLNPFSTI*NKHTKLYD*YNLKI FYTEGLVITLEDVMH**KSTHHFLLVFNVLETVK *YCRTCLLL*KLNVRAE*IKVSRHICIEAKVFY SNNCLLNTTTFSLYCI*LWPTMLYLLFIKL*LF YYCLCQMLTAKLGVT*SIF*KHG*IYFSINYLMK TKF*KPQVLIVIK*HAAILDC*SFC*YFGCN*N YVLSHVKSLINSIYQ*YRSQYLL*DQWSGNSLL YRINWSLCLKKKNFFLTILKLLLNGIILTYSK*G L*GTGC*SIFSEEWICRSLSFYRNIVLTQVLNY FFYELVYFLKFKHILLITLFLEHPALLLTFQQG KME*SGNFFR*VKYPQVSFLPKKGKVIEMGVASG VSG*RWVLGVAVKENNGESLF*TSTYT*SKKQS NLL*STLPLERSIKKSEDGFRKKHEFRNIQNGF CGRFNIMNSQIFKE*ET**LVEMPETVPGSSCV* H*ENSKSVKSQILMKSHRCKDSSVEENIPFLQK EMSIYGVCGK*GKNSYAYESQVVSGRE*SSKLD YHLSNLE*FEIEKKYLIWIWIC*DMHSLFFV*YW KIKML*FGYGFFCIHYLPIEERGKSIMQLLSKP FIFWI*YLKSHEQLHSAYQKAFIK*LLAEDVSK EILH*GHLSE*CLCLFRVG*PPTPGVKESYCLSF PNS*DYRHAPGHVFYL*LLKPR*RNHIFAHSEE TFGH*LVLIFSDCPVFIRLERPCVGHS*YKPLS LLSKPAHLRFHNFLIVLTQNELRAFHFLFVSSQR VGSASAFACQDFVLK*MLFGN*NSCQLSCNFDV TEVGW*FILLILWFFYHIEKAKYSFV*IF

Sequence TPD52.kAug05

CGGGCGGCGCCGCCGCGCCCGACGCCTCTGGGGGTGGGGACTTCGCGGGAGTCGCCGGCGGAGGCGAGGAGGAGCTCTGCGCGGCGCGGCGGGCGATCCG AGCCGGGACGGGCTGCAGGCGGGGGTGCTGCAGAGGACACGAGGCGGCGGGCTGGAGACATGGACCGCGGCGAGCAAGGTCTGCTGAGAACAGACCCAGT CCCTGAGGAAGGAGAAGATGTTGCTGCCACGATCAGTGCCACAGAGACCCTCTCGGAAGAGGAGCAGGAAGAGCTAAGAAGAGAACTTGCAAAGGTAGAA GAAGAAATCCAGACTCTGTCTCAAGTGTTAGCAGCAAAAGAGAAGCATCTAGCAGAGATCAAGCGGAAACTTGGAATCAATTCTCTACAGGAACTAAAAC AGAACATTGCCAAAGGGTGGCAAGACGTGACAGCAACATCTGCGAGGAGCAAGCTTCTAGCAGCAGAAACCGAACTGCTCTGTCTTCTGTATTGAGAGCC ATCTGCAGAGCTGTTACAAGAAGACATCTGAAACCTTATCCCAGGCTGGACAGAAGGCCTCAGCTGCTTTTTCGTCTGTTGGCTCAGTCATCACCAAAAA GCTGGAAGATGTAAAATTGCAAGCCTTTTCACATTCCTTTAGTATACGTTCCATTCAGCATTCAATTAGCATGCCTGCTATGAGAAACTCCCCAACTTTT AAATCATTTGAAGAAAAGGTCGAAAACTTAAAGTCTAAAGTAGGGGGAACCAAGCCTGCTGGTGGTGATTTTGGAGAAGTCTTGAATTCGGCTGCAAATG CTAGTGCCACCACCACGGAGCCTCTTCCAGAAAAGACACAGGAGAGCCTGTGAGATTCCTACCTTTGTTCTGCTACCCACTGCCAGATGCTGCAAGCGAG GTCCAAGCACATCTTGTCAACATGCATTGCCATGAATTTCTACCAGATGTGCTTTTATTTAGCTTTACATATTCCTTTGACCAAATAGTTTGTGGGTTAA ACAAAATGAAAATATCTTCACCTCTATTCTTGGGAAACACCCTTTAGTGTACATTTATGTTCCTTTATTTAGGAAACACCATTATAAAAACACTTATAGT AAATGGGGACATTCACTATAATGATCTAAGAAGCTACAGATTGTCATAGTTGTTTTCCTGCTTTACAAAATTGCTCCAGATCTGGAATGCCAGTTTGACC TTTGTCTTCTATAATATTTCCTTTTTTTCCCCTCTTTGAATCTCTGTATATTTGATTCTTAACTAAAATTGTTCTCTTAAATATTCTGAATCCTGGTAAT TAAAAGTTTGGGTGTATTTTCTTTACCTCCAAGGAAAGAACTACTAGCTACAAAAAATATTTTGGAATAAGCATTGTTTTGGTATAAGGTACATATTTTG GTTGAAGACACCAGACTGAAGTAAACAGCTGTGCATCCAATTTATTATAGTTTTGTAAGTAACAATATGTAATCAAACTTCTAGGTGACTTGAGAGTGGA ACCTCCTATATCATTATTTAGCACCGTTTGTGACAGTAACCATTTCAGTGTATTGTTTATTATACCACTTATATCAACTTATTTTTCACCAGGTTAAAAT TTTAATTTCTACAAAATAACATTCTGAATCAAGCACACTGTATGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAGTTCAAAAGCCTGAAAG TTTAGATCTAGAAGCTGGTAAAAATGACAATATCAATCACATTAGGGGAACCATTGTTGTCTTCACTTAATCCATTTAGCACTATTTAAAATAAGCACAC CAAGTTATATGACTAATATAACTTGAAAATTTTTTATACTGAGGGGTTGGTGATAACTCTTGAGGATGTAATGCATTAATAAAAATCAACTCATCATTTT CTACTTGTTTTCAATGTGTTGGAAACTGTAAAATGATACTGTAGAACCTGTCTCCTACTTTGAAAACTGAATGTCAGGGCTGAGTGAATCAAAGTGTCTA GACATATTTGCATAGAGGCCAAGGTATTCTATTCTAATAACTGCTTACTCAACACTACCACCTTTTCCTTATACTGTATATGATTATGGCCTACAATGTT GTATTTGTTATTTATTAAATTGTGATTGTTTTATTATTGTTTATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCATTTTTTAAAAGCATGGC TAGATTTACTTCAGTATAAATTATCTTATGAAAACCAAATTTTAAAAGCCACAGGTGTTGATTGTTATAAAATAACATGCTGCCATTCTTGATTGCTAGA GTTTTTGTTAGTACTTTGGATGCAATTAAAACTATGTGCTATCACATGTGAAAAGCTTAATAAATTCCATCTATCAGTAGTATAGGTCTCAATATTTATT ATGAGACCAGTGGTCTGGAAACAGCTTGTTGTACCGAATCAACTGGAGTCTATGCTTAAAAAAAAAAAATTTTTTTTTAACCATCCTTAAATTATTGCTT AATGGTATCATATTAACATATTCTAAATAAGGGCTTTAAGGCACAGGCTGTTGAAGCATTTTCTCAGAGGAGTGGATCTGTAGAAGTCTGTCTTTCTATA GAAATATTGTGCTTACTCAAGTGTTAAATTATTTTTTCTATGAACTAGTCTACTTCTTAAAATTCAAACATATTCTTTTGATCACATTGTTTCTTGAGCA TCCTGCCCTGCTACTAACTTTTCAACAAGGCAAAATGGAGTAAAGTGGCAATTTCTTTAGATGAGTGAAATACCCTCAAGTCTCTTTTCTGCCCAAAAAG GGAAAAGTGATAGAAATGGGGGTGGCAAGTGGGGTGAGTGGATGAAGGTGGGTATTGGGGGTGGCTGTGAAAGAAAATAATGGAGAATCACTTTTCTAGA CATCTACCTATACTTAATCTAAGAAACAAAGTAATCTACTGTAAAGTACTCTGCCCCTTGAAAGAAGTATTAAAAAGAGTGAGGATGGATTTAGAAAAAA ACATGAATTTAGAAATATTCAAAATGGTTTTTGTGGCAGATTCAATATTATGAATTCACAGATATTTAAAGAATGAGAAACATAGTAATTAGTAGAAATG CCAGAAACAGTTCCTGGTTCCTCTTGTGTTTGACACTAAGAAAATAGCAAGAGTGTGAAATCTCAGATACTTATGAAATCTCACAGATGTAAGGACTCAA GTGTAGAAGAAAATATCCCCTTCTTACAAAAAGAAATGTCAATTTATGGAGTTTGTGGGAAATAGGGCAAGAATTCTTATGCTTATGAGAGCCAAGTAGT CAGTGGAAGAGAGTAGAGCTCAAAACTGGATTATCACCTTAGCAACTTAGAATAGTTTGAAATAGAAAAAAAGTATTTAATTTGGATCTGGATCTGTTAA GATATGCACAGTCTATTTTTTGTATAGTATTGGAAAATAAAAATGCTATAATTTGGGTATGGGTTCTTTTGTATACATTACCTGCCCATTGAGGAAAGGG GCAAGTCCATTATGCAACTTCTCTCCAAACCCTTCATATTCTGGATATGATACTTAAAGAGCCATGAGCAGCTACATTCTGCCTATCAGAAGGCATTTAT TAAATGACTGCTTGCTGAAGATGTGTCCAAAGAGATCCTACATTAAGGTCATTTGTCAGAATGATGTTTGTGTTTGTTTAGAGTTGGCTGACCTCCAACT CCTGGGGTCAAGGAATCCTACTGCCTTAGCTTCCCAAATAGCTAGGACTATAGGCATGCACCCGGCCATGTGTTTTATTTATAGCTCTTAAAGCCCAGAT GAAGAAATCACATTTTTGCCCATAGTGAAGAAACATTTGGCCATTGATTAGTCCTTATTTTCAGTGACTGTCCTGTTTTCATTAGATTAGAGAGACCCTG TGTGGGCCACAGTTAATATAAACCATTATCACTTTTAAGTAAACCTGCACATCTTAGATTTCATAATTTCCTTATTGTTCTGACTCAAAATGAACTAAGA GCTTTTCACTTTTTGTTTGTAAGTTCTCAGAGAGTCGGGTCTGCAAGTGCTTTTGCCTGCCAGGATTTTGTACTCAAATAAATGCTATTTGGCAACTAAA ATTCTTGCCAGCTCTCATGTAATTTTGATGTTACTGAGGTTGGTTGGTAGTTTATTTTATTAATTTTGTGGTTCTTCTATCATATAGAAAAAGCCAAATA CAGTTTTGTTTGAATATTTGT

TPD52.F12 (forward primer)
TPD52.R11 (reverse primer)
Alternative donor

Frame 0

RAAPPRPTPLGVGTSRESPAEARRSSARRGGRS EPGRAAGGGAAEDTRRRAGDMDRGEQGLLRTDPV PEEGEDVAATISATETLSEEEQEELRRELAKVE EEIQTLSQVLAAKEKHLAEIKRKLGINSLQELK QNIAKGWQDVTATSARSKLLAAETELLCLLY*EP SAELLQEDI*NLIPGWTEGLSCFFVCWLSHHQK AGRCKIASLFTFL*YTFHSAFN*HACYEKLPNF *II*RKGRKLKV*SRGNQACWW*FWRSLEFGCKC *CHHHGASSRKDTGEPVRFLPLFCYPLPDAASE VQAHLVNMHCHEFLPDVLLFSFTYSFDQIVCGL NKMKISSPLFLGNTL*CTFMFLYLGNTIIKTLIV NGDIHYNDLRSYRLS*LFSCFTKLLQIWNASLT FVFYNISFFSPL*ISVYLILN*NCSLKYSESW* LKVWVYFLYLQGKNY*LQKIFWNKHCFGIRYIFW LKTPD*SKQLCIQFIIVL*VTICNQTSR*LESG TSYIII*HRL*Q*PFQCIVYYTTYINLFFTRLK F*FLQNNILNQAHCMFSRLNYEHCHQCSVQKPES LDLEAGKNDNINHIRGTIVVFT*SI*HYLK*AH QVI*LI*LENFLY*GVGDNS*GCNALIKINSSF STCFQCVGNCKMIL*NLSPTLKTECQG*VNQSV* TYLHRGQGILF**LLTQHYHLFLILYMIMAYNV VFVIY*IVIVLLLFMPNVNCQAWSDLKHFLKAW LDLLQYKLSYENQILKATGVDCYKITCCHS*LLE FLLVLWMQLKLCAITCEKLNKFHLSVV*VSIFI MRPVVWKQLVVPNQLESMLKKKKFFFNHP*IIA *WYHINIF*IRALRHRLLKHFLRGVDL*KSVFL* KYCAYSSVKLFFL*TSLLLKIQTYSFDHIVS*A SCPATNFSTRQNGVKWQFL*MSEIPSSLFSAQK GKSDRNGGGKWGEWMKVGIGGGCERK*WRITFLD IYLYLI*ETK*STVKYSAP*KKY*KE*GWI*KK T*I*KYSKWFLWQIQYYEFTDI*RMRNIVISRN ARNSSWFLLCLTLRK*QECEISDTYEISQM*GLK CRRKYPLLTKRNVNLWSLWEIGQEFLCL*EPSS QWKRVELKTGLSP*QLRIV*NRKKVFNLDLDLL RYAQSIFCIVLENKNAIIWVWVLLYTLPAH*GKG QVHYATSLQTLHILDMILKEP*AATFCLSEGIY *MTAC*RCVQRDPTLRSFVRMMFVFV*SWLTSN SWGQGILLP*LPK*LGL*ACTRPCVLFIALKAQM KKSHFCP**RNIWPLISPYFQ*LSCFH*IRETL CGPQLI*TIITFK*TCTS*IS*FPYCSDSK*TK SFSLFVCKFSESRVCKCFCLPGFCTQINAIWQLK FLPALM*F*CY*GWLVVYFINFVVLLSYRKSQI QFCLNIC

Frame 1

GRRRRARRLWGWGLRGSRRRRRGGALRGAAGDP SRDGLQAGVLQRTRGGGLETWTAASKVC*EQTQ SLRKEKMLLPRSVPQRPSRKRSRKS*EENLQR*K KKSRLCLKC*QQKRSI*QRSSGNLESILYRN*N RTLPKGGKT*QQHLRGASF*QQKPNCSVFCIES HLQSCYKKTSETLSQAGQKASAAFSSVGSVITKK LEDVKLQAFSHSFSIRSIQHSISMPAMRNSPTF KSFEEKVENLKSKVGGTKPAGGDFGEVLNSAAN ASATTTEPLPEKTQESL*DSYLCSATHCQMLQAR SKHILSTCIAMNFYQMCFYLALHIPLTK*FVG* TK*KYLHLYSWETPFSVHLCSFI*ETPL*KHL* *MGTFTIMI*EATDCHSCFPALQNCSRSGMPV*P LSSIIFPFFPLFESLYI*FLTKIVLLNILNPGN *KFGCIFFTSKERTTSYKKYFGISIVLV*GTYF G*RHQTEVNSCASNLL*FCK*QYVIKLLGDLRVE PPISLFSTVCDSNHFSVLFIIPLISTYFSPG*N FNFYKITF*IKHTVCSVG*TMNTVINVQFKSLK V*I*KLVKMTISITLGEPLLSSLNPFSTI*NKHT KLYD*YNLKIFYTEGLVITLEDVMH**KSTHHF LLVFNVLETVK*YCRTCLLL*KLNVRAE*IKVS RHICIEAKVFYSNNCLLNTTTFSLYCI*LWPTML YLLFIKL*LFYYCLCQMLTAKLGVT*SIF*KHG *IYFSINYLMKTKF*KPQVLIVIK*HAAILDC* SFC*YFGCN*NYVLSHVKSLINSIYQ*YRSQYLL *DQWSGNSLLYRINWSLCLKKKNFFLTILKLLL NGIILTYSK*GL*GTGC*SIFSEEWICRSLSFY RNIVLTQVLNYFFYELVYFLKFKHILLITLFLEH PALLLTFQQGKME*SGNFFR*VKYPQVSFLPKK GKVIEMGVASGVSG*RWVLGVAVKENNGESLF* TSTYT*SKKQSNLL*STLPLERSIKKSEDGFRKK HEFRNIQNGFCGRFNIMNSQIFKE*ET**LVEM PETVPGSSCV*H*ENSKSVKSQILMKSHRCKDS SVEENIPFLQKEMSIYGVCGK*GKNSYAYESQVV SGRE*SSKLDYHLSNLE*FEIEKKYLIWIWIC* DMHSLFFV*YWKIKML*FGYGFFCIHYLPIEER GKSIMQLLSKPFIFWI*YLKSHEQLHSAYQKAFI K*LLAEDVSKEILH*GHLSE*CLCLFRVG*PPT PGVKESYCLSFPNS*DYRHAPGHVFYL*LLKPR *RNHIFAHSEETFGH*LVLIFSDCPVFIRLERPC VGHS*YKPLSLLSKPAHLRFHNFLIVLTQNELR AFHFLFVSSQRVGSASAFACQDFVLK*MLFGN* NSCQLSCNFDVTEVGW*FILLILWFFYHIEKAKY SFV*IF

Frame 2

GGAAAPDASGGGDFAGVAGGGEEELCAARRAIR AGTGCRRGCCRGHEAAGWRHGPRRARSAENRPS P*GRRRCCCHDQCHRDPLGRGAGRAKKRTCKGR RRNPDSVSSVSSKREASSRDQAETWNQFSTGTKT EHCQRVARRDSNICEEQASSSRNRTALSSVLRA ICRAVTRRHLKPYPRLDRRPQLLFRLLAQSSPK SWKM*NCKPFHIPLVYVPFSIQLACLL*ETPQLL NHLKKRSKT*SLK*GEPSLLVVILEKS*IRLQM LVPPPRSLFQKRHRRACEIPTFVLLPTARCCKR GPSTSCQHALP*ISTRCAFI*LYIFL*PNSLWVK QNENIFTSILGKHPLVYIYVPLFRKHHYKNTYS KWGHSL**SKKLQIVIVVFLLYKIAPDLECQFD LCLL*YFLFFPSLNLCIFDS*LKLFS*IF*ILVI KSLGVFSLPPRKELLATKNILE*ALFWYKVHIL VEDTRLK*TAVHPIYYSFVSNNM*SNF*VT*EW NLLYHYLAPFVTVTISVYCLLYHLYQLIFHQVKI LISTK*HSESSTLYVQ*VEL*TLSSMFSSKA*K FRSRSW*K*QYQSH*GNHCCLHLIHLALFKIST PSYMTNIT*KFFILRGW**LLRM*CINKNQLIIF YLFSMCWKL*NDTVEPVSYFEN*MSGLSESKCL DIFA*RPRYSILITAYSTLPPFPYTVYDYGLQC CICYLLNCDCFIIVYAKC*LPSLE*PKAFFKSMA RFTSV*IIL*KPNFKSHRC*LL*NNMLPFLIAR VFVSTLDAIKTMCYHM*KA**IPSISSIGLNIY YETSGLETACCTESTGVYA*KKKIFF*PSLNYCL MVSY*HILNKGFKAQAVEAFSQRSGSVEVCLSI EILCLLKC*IIFSMN*STS*NSNIFF*SHCFLS ILPCY*LFNKAKWSKVAISLDE*NTLKSLFCPKR EK**KWGWQVG*VDEGGYWGWL*KKIMENHFSR HLPILNLRNKVIYCKVLCPLKEVLKRVRMDLEK NMNLEIFKMVFVADSIL*IHRYLKNEKHSN**KC QKQFLVPLVFDTKKIARV*NLRYL*NLTDVRTQ V*KKISPSYKKKCQFMEFVGNRARILMLMRAK* SVEESRAQNWIITLAT*NSLK*KKSI*FGSGSVK ICTVYFLYSIGK*KCYNLGMGSFVYITCPLRKG ASPLCNFSPNPSYSGYDT*RAMSSYILPIRRHL LNDCLLKMCPKRSYIKVICQNDVCVCLELADLQL LGSRNPTALASQIARTIGMHPAMCFIYSS*SPD EEITFLPIVKKHLAID*SLFSVTVLFSLD*RDP VWATVNINHYHF*VNLHILDFIISLLF*LKMN*E LFTFCL*VLRESGLQVLLPARILYSNKCYLATK ILASSHVILMLLRLVGSLFY*FCGSSII*KKPN TVLFEYL

Sequence TPD52.qAug05

TAAAACAGAACATTGCCAAAGGGTGGCAAGACGTGACAGCAACATCTGCGAGGAGCAAGCTTCTAGCAGCAGAAACCGAACTGCTCTGTCTTCTGTATTG AGAGCCATCTGCAGAGCTGTTACAAGAAGACATCTGAAACCTTATCCCAGGCTGGACAGAAGGCCTCAGCTGCTTTTTCGTCTGTTGGCTCAGTCATCAC CAAAAAGCTGGAAGATGTAAAATTGCAAGCCTTTTCACATTCCTTTAGGTAAGGAGAGTTTGAACCATCACTGCAGTCCAGCAGTTGTATAAGAAAAGTG TGCTGAGATTAGATTACCATGCTACGTTGGATGATCGTGTTTGTCCATATACGTTCCATTCAGCATTCAATTAGCATGCCTGCTATGAGAAACTCCCCAA CTTTTAAATCATTTGAAGAAAAGGTCGAAAACTTAAAGGCAA

TPD52.F12 (forward primer)
TPD52.R11 (reverse primer)
Alternative donor

Frame 0

*NRTLPKGGKT*QQHLRGASF*QQKPNCSVFCI ESHLQSCYKKTSETLSQAGQKASAAFSSVGSVIT KKLEDVKLQAFSHSFR*GEFEPSLQSSSCIRKV C*D*ITMLRWMIVFVHIRSIQHSISMPAMRNSP TFKSFEEKVENLKA

Frame 1

KTEHCQRVARRDSNICEEQASSSRNRTALSSVL RAICRAVTRRHLKPYPRLDRRPQLLFRLLAQSS PKSWKM*NCKPFHIPLGKESLNHHCSPAVV*EKC AEIRLPCYVG*SCLSIYVPFSIQLACLL*ETPQ LLNHLKKRSKT*RQ

Frame 2

KQNIAKGWQDVTATSARSKLLAAETELLCLLY* EPSAELLQEDI*NLIPGWTEGLSCFFVCWLSHH QKAGRCKIASLFTFL*VRRV*TITAVQQLYKKS VLRLDYHATLDDRVCPYTFHSAFN*HACYEKLPN F*II*RKGRKLKG