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Sequences

Sequence TPD52.fAug05

GGCGAAGGGGCTAGCGTCAGGGGCTGGCGGCCGTGATGCGCTTTGGGTGGAAAGCCGCGTGAAGGGAGCACGGCGCAGCCAGCTCCTGTAAGTGACTGGA
ACCAGGAACAGAGGACAGTGAGTCGCTCTCGTCCCTCCAGGTCTGCTGAGAACAGACCCAGTCCCTGAGGAAGGAGAAGATGTTGCTGCCACGATCAGTG
CCACAGAGACCCTCTCGGAAGAGGAGCAGGAAGAGCTAAGAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTGTTAGCAGCAAA
AGAGAAGCATCTAGCAGAGATCAAGCGGAAACTTGGAATCAATTCTCTACAGGAACTAAAACAGAACATTGCCAAAGGGTGGCAAGACGTGACAGCAACA
TCTGCTTACAAGAAGACATCTGAAACCTTATCCCAGGCTGGACAGAAGGCCTCAGCTGCTTTTTCGTCTGTTGGCTCAGTCATCACCAAAAAGCTGGAAG
ATGTAAATATACGTTCCATTCAGCATTCAATTAGCATGCCTGCTATGAGAAACTCCCCAACTTTTAAATCATTTGAAGAAAAGGTCGAAAACTTAAAGTC
TAAAGTAGGGGGAACCAAGCCTGCTGGTGGTGATTTTGGAGAAGTCTTGAATTCGGCTGCAAATGCTAGTGCCACCACCACGGAGCCTCTTCCAGAAAAG
ACACAGGAGAGCCTGTGAGATTCCTACCTTTGTTCTGCTACCCACTGCCAGATGCTGCAAGCGAGGTCCAAGCACATCTTGTCAACATGCATTGCCATGA
ATTTCTACCAGATGTGCTTTTATTTAGCTTTACATATTCCTTTGACCAAATAGTTTGTGGGTTAAACAAAATGAAAATATCTTCACCTCTATTCTTGGGA
AACACCCTTTAGTGTACATTTATGTTCCTTTATTTAGGAAACACCATTATAAAAACACTTATAGTAAATGGGGACATTCACTATAATGATCTAAGAAGCT
ACAGATTGTCATAGTTGTTTTCCTGCTTTACAAAATTGCTCCAGATCTGGAATGCCAGTTTGACCTTTGTCTTCTATAATATTTCCTTTTTTTCCCCTCT
TTGAATCTCTGTATATTTGATTCTTAACTAAAATTGTTCTCTTAAATATTCTGAATCCTGGTAATTAAAAGTTTGGGTGTATTTTCTTTACCTCCAAGGA
AAGAACTACTAGCTACAAAAAATATTTTGGAATAAGCATTGTTTTGGTATAAGGTACATATTTTGGTTGAAGACACCAGACTGAAGTAAACAGCTGTGCA
TCCAATTTATTATAGTTTTGTAAGTAACAATATGTAATCAAACTTCTAGGTGACTTGAGAGTGGAACCTCCTATATCATTATTTAGCACCGTTTGTGACA
GTAACCATTTCAGTGTATTGTTTATTATACCACTTATATCAACTTATTTTTCACCAGGTTAAAATTTTAATTTCTACAAAATAACATTCTGAATCAAGCA
CACTGTATGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAGTTCAAAAGCCTGAAAGTTTAGATCTAGAAGCTGGTAAAAATGACAATATCA
ATCACATTAGGGGAACCATTGTTGTCTTCACTTAATCCATTTAGCACTATTTAAAATAAGCACACCAAGTTATATGACTAATATAACTTGAAAATTTTTT
ATACTGAGGGGTTGGTGATAACTCTTGAGGATGTAATGCATTAATAAAAATCAACTCATCATTTTCTACTTGTTTTCAATGTGTTGGAAACTGTAAAATG
ATACTGTAGAACCTGTCTCCTACTTTGAAAACTGAATGTCAGGGCTGAGTGAATCAAAGTGTCTAGACATATTTGCATAGAGGCCAAGGTATTCTATTCT
AATAACTGCTTACTCAACACTACCACCTTTTCCTTATACTGTATATGATTATGGCCTACAATGTTGTATTTGTTATTTATTAAATTGTGATTGTTTTATT
ATTGTTTATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCATTTTTTAAAAGCATGGCTAGATTTACTTCAGTATAAATTATCTTATGAAAAC
CAAATTTTAAAAGCCACAGGTGTTGATTGTTATAAAATAACATGCTGCCATTCTTGATTGCTAGAGTTTTTGTTAGTACTTTGGATGCAATTAAAACTAT
GTGCTATCACATGTGAAAAGCTTAATAAATTCCATCTATCAGTAGTATAGGTCTCAATATTTATTATGAGACCAGTGGTCTGGAAACAGCTTGTTGTACC
GAATCAACTGGAGTCTATGCTTAAAAAAAAAAAATTTTTTTTTAACCATCCTTAAATTATTGCTTAATGGTATCATATTAACATATTCTAAATAAGGGCT
TTAAGGCACAGGCTGTTGAAGCATTTTCTCAGAGGAGTGGATCTGTAGAAGTCTGTCTTTCTATAGAAATATTGTGCTTACTCAAGTGTTAAATTATTTT
TTCTATGAACTAGTCTACTTCTTAAAATTCAAACATATTCTTTTGATCACATTGTTTCTTGAGCATCCTGCCCTGCTACTAACTTTTCAACAAGGCAAAA
TGGAGTAAAGTGGCAATTTCTTTAGATGAGTGAAATACCCTCAAGTCTCTTTTCTGCCCAAAAAGGGAAAAGTGATAGAAATGGGGGTGGCAAGTGGGGT
GAGTGGATGAAGGTGGGTATTGGGGGTGGCTGTGAAAGAAAATAATGGAGAATCACTTTTCTAGACATCTACCTATACTTAATCTAAGAAACAAAGTAAT
CTACTGTAAAGTACTCTGCCCCTTGAAAGAAGTATTAAAAAGAGTGAGGATGGATTTAGAAAAAAACATGAATTTAGAAATATTCAAAATGGTTTTTGTG
GCAGATTCAATATTATGAATTCACAGATATTTAAAGAATGAGAAACATAGTAATTAGTAGAAATGCCAGAAACAGTTCCTGGTTCCTCTTGTGTTTGACA
CTAAGAAAATAGCAAGAGTGTGAAATCTCAGATACTTATGAAATCTCACAGATGTAAGGACTCAAGTGTAGAAGAAAATATCCCCTTCTTACAAAAAGAA
ATGTCAATTTATGGAGTTTGTGGGAAATAGGGCAAGAATTCTTATGCTTATGAGAGCCAAGTAGTCAGTGGAAGAGAGTAGAGCTCAAAACTGGATTATC
ACCTTAGCAACTTAGAATAGTTTGAAATAGAAAAAAAGTATTTAATTTGGATCTGGATCTGTTAAGATATGCACAGTCTATTTTTTGTATAGTATTGGAA
AATAAAAATGCTATAATTTGGGTATGGGTTCTTTTGTATACATTACCTGCCCATTGAGGAAAGGGGCAAGTCCATTATGCAACTTCTCTCCAAACCCTTC
ATATTCTGGATATGATACTTAAAGAGCCATGAGCAGCTACATTCTGCCTATCAGAAGGCATTTATTAAATGACTGCTTGCTGAAGATGTGTCCAAAGAGA
TCCTACATTAAGGTCATTTGTCAGAATGATGTTTGTGTTTGTTTAGAGTTGGCTGACCTCCAACTCCTGGGGTCAAGGAATCCTACTGCCTTAGCTTCCC
AAATAGCTAGGACTATAGGCATGCACCCGGCCATGTGTTTTATTTATAGCTCTTAAAGCCCAGATGAAGAAATCACATTTTTGCCCATAGTGAAGAAACA
TTTGGCCATTGATTAGTCCTTATTTTCAGTGACTGTCCTGTTTTCATTAGATTAGAGAGACCCTGTGTGGGCCACAGTTAATATAAACCATTATCACTTT
TAAGTAAACCTGCACATCTTAGATTTCATAATTTCCTTATTGTTCTGACTCAAAATGAACTAAGAGCTTTTCACTTTTTGTTTGTAAGTTCTCAGAGAGT
CGGGTCTGCAAGTGCTTTTGCCTGCCAGGATTTTGTACTCAAATAAATGCTATTTGGCAACTAAAATTCTTGCCAGCTCTCATGTAATTTTGATGTTACT
GAGGTTGGTTGGTAGTTTATTTTATTAATTTTGTGGTTCTTCTATCATATAGAAAAAGCCAAATACAGTTTTGTTTGAATATTTGT
  • TPD52.F12 (forward primer)
  • TPD52.R11 (reverse primer)
  • Single exon skipping

Frame 0

GEGASVRGWRP*CALGGKPREGSTAQPAPVSDW
NQEQRTVSRSRPSRSAENRPSP*GRRRCCCHDQC
HRDPLGRGAGRAKKRTCKGRRRNPDSVSSVSSK
REASSRDQAETWNQFSTGTKTEHCQRVARRDSN
ICLQEDI*NLIPGWTEGLSCFFVCWLSHHQKAGR
CKYTFHSAFN*HACYEKLPNF*II*RKGRKLKV
*SRGNQACWW*FWRSLEFGCKC*CHHHGASSRK
DTGEPVRFLPLFCYPLPDAASEVQAHLVNMHCHE
FLPDVLLFSFTYSFDQIVCGLNKMKISSPLFLG
NTL*CTFMFLYLGNTIIKTLIVNGDIHYNDLRS
YRLS*LFSCFTKLLQIWNASLTFVFYNISFFSPL
*ISVYLILN*NCSLKYSESW*LKVWVYFLYLQG
KNY*LQKIFWNKHCFGIRYIFWLKTPD*SKQLC
IQFIIVL*VTICNQTSR*LESGTSYIII*HRL*Q
*PFQCIVYYTTYINLFFTRLKF*FLQNNILNQA
HCMFSRLNYEHCHQCSVQKPESLDLEAGKNDNI
NHIRGTIVVFT*SI*HYLK*AHQVI*LI*LENFL
Y*GVGDNS*GCNALIKINSSFSTCFQCVGNCKM
IL*NLSPTLKTECQG*VNQSV*TYLHRGQGILF
**LLTQHYHLFLILYMIMAYNVVFVIY*IVIVLL
LFMPNVNCQAWSDLKHFLKAWLDLLQYKLSYEN
QILKATGVDCYKITCCHS*LLEFLLVLWMQLKL
CAITCEKLNKFHLSVV*VSIFIMRPVVWKQLVVP
NQLESMLKKKKFFFNHP*IIA*WYHINIF*IRA
LRHRLLKHFLRGVDL*KSVFL*KYCAYSSVKLF
FL*TSLLLKIQTYSFDHIVS*ASCPATNFSTRQN
GVKWQFL*MSEIPSSLFSAQKGKSDRNGGGKWG
EWMKVGIGGGCERK*WRITFLDIYLYLI*ETK*
STVKYSAP*KKY*KE*GWI*KKT*I*KYSKWFLW
QIQYYEFTDI*RMRNIVISRNARNSSWFLLCLT
LRK*QECEISDTYEISQM*GLKCRRKYPLLTKR
NVNLWSLWEIGQEFLCL*EPSSQWKRVELKTGLS
P*QLRIV*NRKKVFNLDLDLLRYAQSIFCIVLE
NKNAIIWVWVLLYTLPAH*GKGQVHYATSLQTL
HILDMILKEP*AATFCLSEGIY*MTAC*RCVQRD
PTLRSFVRMMFVFV*SWLTSNSWGQGILLP*LP
K*LGL*ACTRPCVLFIALKAQMKKSHFCP**RN
IWPLISPYFQ*LSCFH*IRETLCGPQLI*TIITF
K*TCTS*IS*FPYCSDSK*TKSFSLFVCKFSES
RVCKCFCLPGFCTQINAIWQLKFLPALM*F*CY
*GWLVVYFINFVVLLSYRKSQIQFCLNIC

Frame 1

AKGLASGAGGRDALWVESRVKGARRSQLL*VTG
TRNRGQ*VALVPPGLLRTDPVPEEGEDVAATIS
ATETLSEEEQEELRRELAKVEEEIQTLSQVLAAK
EKHLAEIKRKLGINSLQELKQNIAKGWQDVTAT
SAYKKTSETLSQAGQKASAAFSSVGSVITKKLE
DVNIRSIQHSISMPAMRNSPTFKSFEEKVENLKS
KVGGTKPAGGDFGEVLNSAANASATTTEPLPEK
TQESL*DSYLCSATHCQMLQARSKHILSTCIAM
NFYQMCFYLALHIPLTK*FVG*TK*KYLHLYSWE
TPFSVHLCSFI*ETPL*KHL**MGTFTIMI*EA
TDCHSCFPALQNCSRSGMPV*PLSSIIFPFFPL
FESLYI*FLTKIVLLNILNPGN*KFGCIFFTSKE
RTTSYKKYFGISIVLV*GTYFG*RHQTEVNSCA
SNLL*FCK*QYVIKLLGDLRVEPPISLFSTVCD
SNHFSVLFIIPLISTYFSPG*NFNFYKITF*IKH
TVCSVG*TMNTVINVQFKSLKV*I*KLVKMTIS
ITLGEPLLSSLNPFSTI*NKHTKLYD*YNLKIF
YTEGLVITLEDVMH**KSTHHFLLVFNVLETVK*
YCRTCLLL*KLNVRAE*IKVSRHICIEAKVFYS
NNCLLNTTTFSLYCI*LWPTMLYLLFIKL*LFY
YCLCQMLTAKLGVT*SIF*KHG*IYFSINYLMKT
KF*KPQVLIVIK*HAAILDC*SFC*YFGCN*NY
VLSHVKSLINSIYQ*YRSQYLL*DQWSGNSLLY
RINWSLCLKKKNFFLTILKLLLNGIILTYSK*GL
*GTGC*SIFSEEWICRSLSFYRNIVLTQVLNYF
FYELVYFLKFKHILLITLFLEHPALLLTFQQGK
ME*SGNFFR*VKYPQVSFLPKKGKVIEMGVASGV
SG*RWVLGVAVKENNGESLF*TSTYT*SKKQSN
LL*STLPLERSIKKSEDGFRKKHEFRNIQNGFC
GRFNIMNSQIFKE*ET**LVEMPETVPGSSCV*H
*ENSKSVKSQILMKSHRCKDSSVEENIPFLQKE
MSIYGVCGK*GKNSYAYESQVVSGRE*SSKLDY
HLSNLE*FEIEKKYLIWIWIC*DMHSLFFV*YWK
IKML*FGYGFFCIHYLPIEERGKSIMQLLSKPF
IFWI*YLKSHEQLHSAYQKAFIK*LLAEDVSKE
ILH*GHLSE*CLCLFRVG*PPTPGVKESYCLSFP
NS*DYRHAPGHVFYL*LLKPR*RNHIFAHSEET
FGH*LVLIFSDCPVFIRLERPCVGHS*YKPLSL
LSKPAHLRFHNFLIVLTQNELRAFHFLFVSSQRV
GSASAFACQDFVLK*MLFGN*NSCQLSCNFDVT
EVGW*FILLILWFFYHIEKAKYSFV*IF

Frame 2

RRG*RQGLAAVMRFGWKAA*REHGAASSCK*LE
PGTEDSESLSSLQVC*EQTQSLRKEKMLLPRSV
PQRPSRKRSRKS*EENLQR*KKKSRLCLKC*QQ
KRSI*QRSSGNLESILYRN*NRTLPKGGKT*QQH
LLTRRHLKPYPRLDRRPQLLFRLLAQSSPKSWK
M*IYVPFSIQLACLL*ETPQLLNHLKKRSKT*S
LK*GEPSLLVVILEKS*IRLQMLVPPPRSLFQKR
HRRACEIPTFVLLPTARCCKRGPSTSCQHALP*
ISTRCAFI*LYIFL*PNSLWVKQNENIFTSILG
KHPLVYIYVPLFRKHHYKNTYSKWGHSL**SKKL
QIVIVVFLLYKIAPDLECQFDLCLL*YFLFFPS
LNLCIFDS*LKLFS*IF*ILVIKSLGVFSLPPR
KELLATKNILE*ALFWYKVHILVEDTRLK*TAVH
PIYYSFVSNNM*SNF*VT*EWNLLYHYLAPFVT
VTISVYCLLYHLYQLIFHQVKILISTK*HSESS
TLYVQ*VEL*TLSSMFSSKA*KFRSRSW*K*QYQ
SH*GNHCCLHLIHLALFKISTPSYMTNIT*KFF
ILRGW**LLRM*CINKNQLIIFYLFSMCWKL*N
DTVEPVSYFEN*MSGLSESKCLDIFA*RPRYSIL
ITAYSTLPPFPYTVYDYGLQCCICYLLNCDCFI
IVYAKC*LPSLE*PKAFFKSMARFTSV*IIL*K
PNFKSHRC*LL*NNMLPFLIARVFVSTLDAIKTM
CYHM*KA**IPSISSIGLNIYYETSGLETACCT
ESTGVYA*KKKIFF*PSLNYCLMVSY*HILNKG
FKAQAVEAFSQRSGSVEVCLSIEILCLLKC*IIF
SMN*STS*NSNIFF*SHCFLSILPCY*LFNKAK
WSKVAISLDE*NTLKSLFCPKREK**KWGWQVG
*VDEGGYWGWL*KKIMENHFSRHLPILNLRNKVI
YCKVLCPLKEVLKRVRMDLEKNMNLEIFKMVFV
ADSIL*IHRYLKNEKHSN**KCQKQFLVPLVFD
TKKIARV*NLRYL*NLTDVRTQV*KKISPSYKKK
CQFMEFVGNRARILMLMRAK*SVEESRAQNWII
TLAT*NSLK*KKSI*FGSGSVKICTVYFLYSIG
K*KCYNLGMGSFVYITCPLRKGASPLCNFSPNPS
YSGYDT*RAMSSYILPIRRHLLNDCLLKMCPKR
SYIKVICQNDVCVCLELADLQLLGSRNPTALAS
QIARTIGMHPAMCFIYSS*SPDEEITFLPIVKKH
LAID*SLFSVTVLFSLD*RDPVWATVNINHYHF
*VNLHILDFIISLLF*LKMN*ELFTFCL*VLRE
SGLQVLLPARILYSNKCYLATKILASSHVILMLL
RLVGSLFY*FCGSSII*KKPNTVLFEYL

Sequence TPD52.qAug05

TAAAACAGAACATTGCCAAAGGGTGGCAAGACGTGACAGCAACATCTGCGAGGAGCAAGCTTCTAGCAGCAGAAACCGAACTGCTCTGTCTTCTGTATTG
AGAGCCATCTGCAGAGCTGTTACAAGAAGACATCTGAAACCTTATCCCAGGCTGGACAGAAGGCCTCAGCTGCTTTTTCGTCTGTTGGCTCAGTCATCAC
CAAAAAGCTGGAAGATGTAAAATTGCAAGCCTTTTCACATTCCTTTAGGTAAGGAGAGTTTGAACCATCACTGCAGTCCAGCAGTTGTATAAGAAAAGTG
TGCTGAGATTAGATTACCATGCTACGTTGGATGATCGTGTTTGTCCATATACGTTCCATTCAGCATTCAATTAGCATGCCTGCTATGAGAAACTCCCCAA
CTTTTAAATCATTTGAAGAAAAGGTCGAAAACTTAAAGGCAA
  • TPD52.F12 (forward primer)
  • TPD52.R11 (reverse primer)
  • Single exon skipping

Frame 0

*NRTLPKGGKT*QQHLRGASF*QQKPNCSVFCI
ESHLQSCYKKTSETLSQAGQKASAAFSSVGSVIT
KKLEDVKLQAFSHSFR*GEFEPSLQSSSCIRKV
C*D*ITMLRWMIVFVHIRSIQHSISMPAMRNSP
TFKSFEEKVENLKA

Frame 1

KTEHCQRVARRDSNICEEQASSSRNRTALSSVL
RAICRAVTRRHLKPYPRLDRRPQLLFRLLAQSS
PKSWKM*NCKPFHIPLGKESLNHHCSPAVV*EKC
AEIRLPCYVG*SCLSIYVPFSIQLACLL*ETPQ
LLNHLKKRSKT*RQ

Frame 2

KQNIAKGWQDVTATSARSKLLAAETELLCLLY*
EPSAELLQEDI*NLIPGWTEGLSCFFVCWLSHH
QKAGRCKIASLFTFL*VRRV*TITAVQQLYKKS
VLRLDYHATLDDRVCPYTFHSAFN*HACYEKLPN
F*II*RKGRKLKG

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