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Sequences

Sequence CTSB.qAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGGTCTTGCTTTGTCTCCCAGGCTGGTGTGCAGTGGTGCGACCACGGCTCACGGCAGCCTCAGC CACCCAGATGTAAGCGATCTGGTTCCCACCTCAGCCTCCCGAGTAGATACTTCTGAAAATAGAAATGATGACTCTGGGATGCAAACGTTGGCTGTCCTAT GTATAAGGAGATGGCTTTTCACGCTCCCAGTGACTGAGGAAGTTTCTCCCAGATGGCGCTGCTCTGAGCCTGGTGCAGGGTGTCTTCAGGCCTATGGAGA GCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAGATGATTGGCAGGTGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCTCCC TCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTG GCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTT ACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCT GCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGGGGGATTCGGGGTGGGGACAACTCTGACAA GGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAATGATGGCGAGGATTAGGTGGAGTGGCGGGC AGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCCCGTCCGCAAATACCAGGAGCTCTCACTGC TACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAAATACACGTAACAGAAATGTAATAATCTTA GCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGGGAT ATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGACTACAGGCATGTGCCACCACGCCCAGCTA ATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTGATCTGCCTTCCTCGGCCTCGCAAAATGCT GGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACATTCACAGTGTTGCTCAGCCACCACCACCAT CTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCCTGGTAAGCCCCACTCTACTTTCTGTCTCT ATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTTTTTGTTAAGAAAAAAACACAGCCAGGTAC GGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTGAGACCAACCTGGCCAACATGGTGAAACCC TGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGG AGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCCTCCAGGAAAAAAAAAAAAAACCACACACA CACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGGGGGTGCTTCTCTTGCTTTAAAAGCTGAGT TGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAAATCACTTGAACCCGGG AGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGCCCCAGGAGCGTCCCCTGGTGTTGAGAGAT CAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGACATGGCAGCCTCACCCGGTGGGGCTCGTTC CTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACAT GCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCCTGGGCCCCAGCTTCTCAGTGGAGGGGACT TTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGCCTGGCAAGGCTGCTGCTTCCTGGGAGGTG CCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAATGTGGTGGAATGTCTGGCCCCTGGTCTGG TTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGCGGGGATGGCTCCACCTTAACAACAAGGTG GTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCGGCTGCAGATCCGAAATGCCTGAGGGCTTA AAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGGTGGTGTTTTTAAGTACCTTAGGGAAGTTT AGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGGTATGGACATGAACAGGAGAGCCTCCTGTCTTCTCCCGGAT CTTTGTGGGCAGGGGTGGGGCTGGCGGTTATTCCCTGCAAGCTGTGCTTATCTAGGGAGCGTCCCTTGGAGGGTTTGGGGTCTGGGAGGTCTGCTCGCAC CACTTGCTGCAGCTGGGGGTGGGTCCACGAGTGGCCTCGGGCACTTGGGTAGCACACAGTGGTCTGGAGAGCTGGTGGTGCTTCTCTCAGAGGTTTTCAT TAGAGGCTGTCTTTTCAGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCAT GTAGGTAAGTGTGTCCCCTTGGCCACTTTCTGGCCAGATGGATTGTTTGAGCAATTAACCATCATGGCTTTATTTGCCTTTATAAACTGGGGGTTGAGAC AGAGGGGCTGCTGAGAGGTGCTAGCCAGGTGCACAGGCCTCTGGCAGGACCTGCCTGGCGTCCATGCTGCAGGCACGAGGCTTGCCTTGCCCCAGGTCTT CTCCGTGGGGGCTGTAGGTTGACTCCGCTTTCTCCCGCGTCCCATCAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCG GCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACGGTAAGGGG CCTGGGGCCTGGCCACGGCGCACGTGGAGGCTGGGGAGCTGCTGCATCCCTCCTCACGCTGCAGCGAAGAGGTCAGGGCTGAGGAGCCTTGGGGTCCCGG AACTCTAGGATAGAGGAGGGGGAGTGATGCCCTCTTGCCAGGAGAAGCAGCACACTCTCCACTTTCTGCCTGTTCCACCCTGAACTCAGCCTCAGCCCTT CCAAACTGGAAGGGACCAAAGCCCTCCTTTTACAAGGGAGTGGCGTGTCCTGGAGTCTTGAGGTATCCTGGCCTCTTCCGGGGCCTCGTGCTCATTGCTC CGTTCTCCCATTTCTGAGCTTCCAGCCCCAGCCCTGTCCTTAGTTCTTCAGGGGAGCCTTCCTTGAGCTCCCCTGGCAGGGCAAGTCCCCTTAGATGCAG TCTTCCCCTGGAGGCCACCAGAAATCAGTGACTGGCTGACCGTGGCGTGCCACGAGGCACGGTCACTGCCCTCCCCAACCCCGAGCTCGGTTCATTTTCC AGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGAC TTCCTGCTCTACAAGTCAGGTGCGTGCTGATGGCTGATGGCAATAGAGGGTGGGGGTCGGGAGGGGAGCCTGGGTGCCAGGATGGTTCATGTTGACCAAT AGGGCTGGATTGGGCAGGCAGGTGAGGGGCTGGGGAAGAGGGCTGTGTGGGGCCTTCCATATAGGCTCACTCCTCTGTGGACCAGCCTGGCACCTGACTG TCTTTGTCCAGGTGTTGTGCCAACACACACAATTCCAGAGGCCTCTCCCAGAGCCTCTGGGCTAGTTCCTTCCCATCCATTAGCGTCCAGCTCATACATG TTCTCTGTGGGAACGCCCCCCTCCCTGGTGGCTGGCCACGCCTGCCCTCGCCTTCCTGGCTTTGCATTGCTTTACCAGCATGTTTCTGTTATAATGGCAG GCCGTTCGTGTTTGCACTGGCTGACACATTTTCTTCATCTTTCTCTTTGCAAAGGCCTAGATCGTGCCTCACACCAGTGGATGAACACACAGGGAGGACC TTGCCCTGGGTAGTGCCATAGGGACTCCAACCAAGGAATCTGGACAGTCCCCATCCCCAAGTCCTGTCTTAGAAATCCCTTGCTCCAGATGAACAGTCTT TGCTGACTGCATCTATCCCATTAATATTTTGCTAGATTGGAAACTTGTAAAACAACAGTTTGACAAAGGAAGATGATTCTGGCAAGAAAAAGTTTCTGTG AAGATGGCACTTCTTAGCATAGTCCTCGCCCTTATTATTAAAAGATGCATTTCCACTTTGACAGTGATTTCCCCACCCGAGAGCCTGGTTGGGCAGCCCT GAGCCCCTTAGCCAGTATCTTTCCCATCAGCTAGCATTAGCTGCCACCAGTCCTCCCTTCTCCTCCCTCACCTCTTCAGGGTGTACCTGGAGCAGCTATT AAAACTCTTTAAGCCGTGTAACGCTTTTGACAAACCCTTACTTACATGGAATCCCAGTATAAAGAACAGATGAATCAGTCATCTAGCATTAAATGTTTTA TCAATTAGAATGTATTAGAAATTCTATTTTTAAAGCCAACAAGCACATTTTGTAGGAATCTATTTCAATATTTACTCAACAACTTTGAGTGTGTTGGGCC TTCAAAAATATATTTAATTCCCCAGTATTGTGGGACCCCCCCTGGAGCCTCCTGGTGGAGCAGCAGGTTCCCTCTGGAAGCTGTTTCTCCTTCCCGGAGC ATGACCAGCTGAGTGTGGGGGGTGCTGTGGGGCGTGGGACAGTGGCCCGGTCTCGGTCTCAGCCAGTTCTTCCCTTTTCAGGAGTGTACCAACACGTCAC CGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACTGG GGTGACAATGGTGAGTGGCTGCCCCCTTCCTGCCAAGAACAGTGAATTGTGAGCCACACCCCGTGGCCATCTCGGCTTTCTCTGTTCTACCCACCGCACA GCCTTAAACCCTGGACCTACGGCCAGGCTGTGAGCTCCTCCTAAGTGCCAGGCAGTCAGGAGTTCCCTTTTGCTGTGAGGGCAGACTCTGAGCAGCTTCA GAGCCAACGCCTGCAACAGTTCCCACAGCATCGCGCCAGATCCTGTGATGGGAAGGGTGACCGGGCAGGGGGCTTGCCCGTGGAGGTGTGCCCCACGGCT CCAGAAGCCTTGTGGTGTTGAGGACTGTGCTAGTGGGCCAACAGCAGGAGTGGCCAGGGATGAGTGACTTAAGGTCTTTTAAGGATGAGTCTGACTATAT TGGTTGACCCTTGTCACACTTTAAAAGCACCTTACTTTTTATTCCCAGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGT GGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGGTAGAAATGCA TTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTG GCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTA CCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGA AAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAAC GCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCG TGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAG TGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACC CTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTT GTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCC ATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCA AAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCA ACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTT TAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG GCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCT CACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAAT TTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTA GAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAA GTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCT CTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGT TCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGG AGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCG TCTCAATAATT

CTSB.F6 (forward primer)
CTSB.R2 (reverse primer)
Alternative donor

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC AQAGLQALGCSAGVLLCLPGWCAVVRPRLTAASA TQM*AIWFPPQPPE*ILLKIEMMTLGCKRWLSY V*GDGFSRSQ*LRKFLPDGAALSLVQGVFRPME SSLRGLGLQYLVCIDDWQVDLGSGFQHVAALGLP LLPAGVGQCPEQALFPSPVG*AGQLCQQTEYHV AGRAQLLQRGHELLEEAMWYLPGWAQATPESYV YRGPEAACKLRCTGTMATVSHHQRDQRPGLLWLL LGKALLAGGEALERKWEQHWRVLGDSGWGQL*Q GKL*KLSESQFPSVQKSQSLWP*MMARIRWSGG QSIQQIASPRVQVAKQLPFPDMLARPQIPGALTA TLLQESIPLVSVSCLNFVI*LW*NTRNRNVIIL AIFFCFLFLSFFFFFFFGDGVLLCHPGWSAVVG YWLTATSASWVQAILLPQSPQ*LRLQACATTPS* FLYF**RWGFAMLDSLVSNASPVDLPSSASQNA GITGVSHRAQQLFSSVQFCSIKYIHSVAQPPPP SVPRTLFQLARQKLCPH*HQYCSPW*APLYFLSL *I*LLGTSYKWITTVFIFWVSCVFC*EKNTARY GSSRL*SQHFGRLRQADHLRSGV*DQPGQHGET LSLLKRQNLAGPGGRHL*SQLL*RLRQENCLNPG GVGCSELRLRHCIPAWATKVKLRLQEKKKKPHT HIQTNTTHFADFRNEPSFQAEVHGGASLALKAE LGKSLRQESGASGSSRL*SQHFGRPRWANHLNPG VQDQSGQRSKTPFLPKKIKAEFEPQERPLVLRD QLPTRSEVPLWPFEGTAAEGPARTWQPHPVGLV PAGLRGCGSHL*PDLHPHQCARQRGGVGGGPAHM LWQHVWGRVSQAVLPQRV*C*ETLGPSFSVEGT LRTSWDCCESEVFRGDSESLAGRAWQGCCFLGG A*TKAGPAELSEGWHPSKTVSFRKCGGMSGPWSG LCHRMFLFPAWGTSLGHFLMCS*RGWLHLNNKV VAQNFLLQLPAASPWFRSSGVHWRLQIRNA*GL KKMHGLLP*LVKWESLAILISENWWCF*VP*GSL ASAQLRISVLPGRALVAWSGMDMNRRASCLLPD LCGQGWGWRLFPASCAYLGSVPWRVWGLGGLLA PLAAAGGGSTSGLGHLGSTQWSGELVVLLSEVFI RGCLFSCNGGYPAEAWNFWTRKGLVSGGLYESH VGKCVPLATFWPDGLFEQLTIMALFAFINWGLR QRGC*EVLARCTGLWQDLPGVHAAGTRLALPQVF SVGAVG*LRFLPRPIRVQTVLHPSL*APRQRLP APMHGGGRYPQV*QDL*AWLQPDLQTGQALR*G AWGLATAHVEAGELLHPSSRCSEEVRAEEPWGPG TLG*RRGSDALLPGEAAHSPLSACSTLNSASAL PNWKGPKPSFYKGVACPGVLRYPGLFRGLVLIA PFSHF*ASSPSPVLSSSGEPSLSSPGRASPLRCS LPLEATRNQ*LADRGVPRGTVTALPNPELGSFS RIQFLQRLQ*REGHHGRDLQKRPRGGSFLCVFG LPALQVRCVLMADGNRGWGSGGEPGCQDGSC*PI GLDWAGR*GAGEEGCVGPSI*AHSSVDQPGT*L SLSRCCANTHNSRGLSQSLWASSFPSISVQLIH VLCGNAPLPGGWPRLPSPSWLCIALPACFCYNGR PFVFALADTFSSSFSLQRPRSCLTPVDEHTGRT LPWVVP*GLQPRNLDSPHPQVLS*KSLAPDEQS LLTASIPLIFC*IGNL*NNSLTKEDDSGKKKFL* RWHFLA*SSPLLLKDAFPL*Q*FPHPRAWLGSP EPLSQYLSHQLALAATSPPFSSLTSSGCTWSSY *NSLSRVTLLTNPYLHGIPV*RTDESVI*H*MFY QLECIRNSIFKANKHIL*ESISIFTQQL*VCWA FKNIFNSPVLWDPPWSLLVEQQVPSGSCFSFPE HDQLSVGGAVGRGTVARSRSQPVLPFSGVYQHVT GEMMGGHAIRILGWGVENGTPYWLVANSWNTDW GDNGEWLPPSCQEQ*IVSHTPWPSRLSLFYPPH SLKPWTYGQAVSSS*VPGSQEFPFAVRADSEQLQ SQRLQQFPQHRARSCDGKGDRAGGLPVEVCPTA PEALWC*GLC*WANSRSGQG*VT*GLLRMSLTI LVDPCHTLKAPYFLFPGFFKILRGQDHCGIESEV VAGIPRTDQYWEKI*SAVGLSCQSWGRDRGRNA FYSLSSRKIQVSDRV*RTGLAKHQTCLPRRPSP GYIPACGYSADRPCEPPLPAQSVLPPVD*CRREY LLPQLTVAPSVIHPSPGSKTETQEWKAEFLTG* KFPHQFPQYLQASSFPHLSQKSEERRCWEPFGE RQSPRPPASIEFAMSQPL*SCAQHDSLIEVLFFR ALC*SCG*ASGTAGDLC*FYRLPPYDAAQKETK WSGVVSDPLISTTTRKIV*EKPAFTVFEKLQLH PVKLTRNACANKSFLQLEVYSDGISDPLSLPIDL *LLSLFVPAENHVLELHVLATLGTSS*LLAAPA MFSTMASLPQLVPCHPRQPSL*VPGKLALA*EL KT*LCSIFLLLL*LTE*DSVSQAGVQWRLLSSLQ PAAS*IQAILLLQPSE*LG*QALTNMPG*FLYF *VHTGFHHVGQASFKLPASGGLPASASQSVGIT GVSHWALPVFFISHKSSNKLRIQLIKQAWCLGDL T*PRCYPVGNHIPLDALQPWFGLESGPVYSILN LYATGLHCWSGTLVLCIALV*KHVIAVLGIEQT RRTSNHSTVLPRYTSVLPFPTEVV*G*LFPKAFK FGF*CDSEFRNQMLDQISSENLRNIVGKVC*AS LKCHDEHNSRPSWLTPVIPALWEAKVGG*QGQE FKTSLANMLKPHLY*KYKN*LGMVAHACNPSYLG G*GRRIA*TREAEVAVSQDSASALQPR*QRKAP SQ*

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA RRLGCRLSAAALGSCFVSQAGVQWCDHGSRQPQ PPRCKRSGSHLSLPSRYF*K*K**LWDANVGCPM YKEMAFHAPSD*GSFSQMALL*AWCRVSSGLWR AACVGWACSTWFA*MIGRWI*DPASNMWQLWAS LCCLLVLANARSRPSFHPLSDELVNYVNKRNTTW QAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMF TEDLKLPASFDAREQWPQCPTIKEIRDQGSCGS CWVRPCWLAGKRWRESGSNTGESWGIRGGDNSDK ASYRNFLSPSFHQYKNHNPSGHE*WRGLGGVAG RASSRLQVHVYRWRSSSLSLTCWPVRKYQELSL LLCFKKASL*CQ*AVLILSFNCGKIHVTEM**S* QSSFVFFFFLFFFFFFLEMESCSVTQAGVQWWD IGSLQPLPPGFKQFSCLSLPSS*DYRHVPPRPA NFCIFSRDGVLPCWTAWSQTPHLLICLPRPRKML GLQA*ATVPSNYFQVYSSVALSTFTVLLSHHHH LSPELFFSSQDRNSVPINTNIVAPGKPHSTFCL YEFDS*GPHTSGSQQYLFSG*AVFFVKKKTQPGT VAHACNLNTLGG*GRRIT*GQEFETNLANMVKP CLY*KDKT*LGLVAGTYNPSYYRG*GRRIA*TQ EA*VAVS*DCATAFQPGQQK*NSASRKKKKNHTH TYKLILHILQISEMNPVSRQRFTGVLLLL*KLS WANR*GRRVGQVAHHASNPSTLGGQGGQIT*TR EFKTSLANVARPHFYQRK*KLSLSPRSVPWC*EI SCLQGLRCPCGPLRGLLQRAQPGHGSLTRWGSF LQAFGAVEAISDRICIHTNAHVSVEVSAEDLLT CCGSMCGDG*VRLCFHSGFSAERPWAPASQWRGL *GLPGTAASQKCFGETPRVWQAGPGKAAASWEV PEPRLARQSCLRGGIPAKQSVSENVVECLAPGL VCVIACSFFLLGEPVWGISLCVVSGDGSTLTTRW WHRTFCSSCLQPRLGSAVAVFTGGCRSEMPEGL KKCMGFSPN*LNGNHWRFSFQRTGGVFKYLREV *RLLS*ESVFYPEGHS*PGLVWT*TGEPPVFSRI FVGRGGAGGYSLQAVLI*GASLGGFGVWEVCSH HLLQLGVGPRVASGTWVAHSGLESWWCFSQRFS LEAVFSAVMVAILLKLGTSGQEKAWFLVASMNPM *VSVSPWPLSGQMDCLSN*PSWLYLPL*TGG*D RGAAERC*PGAQASGRTCLASMLQARGLPCPRS SPWGL*VDSAFSRVPSGCRPYSIPPCEHHVNGSR PPCTGEGDTPKCSKICEPGYSPTYKQDKHYGKG PGAWPRRTWRLGSCCIPPHAAAKRSGLRSLGVP EL*DRGGGVMPSCQEKQHTLHFLPVPP*TQPQPF QTGRDQSPPFTREWRVLES*GILASSGASCSLL RSPISELPAPALSLVLQGSLP*APLAGQVPLDA VFPWRPPEISDWLTVACHEARSLPSPTPSSVHFP GYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSD FLLYKSGAC*WLMAIEGGGREGSLGARMVHVDQ *GWIGQAGEGLGKRAVWGLPYRLTPLWTSLAPDC LCPGVVPTHTIPEASPRASGLVPSHPLASSSYM FSVGTPPSLVAGHACPRLPGFALLYQHVSVIMA GRSCLHWLTHFLHLSLCKGLDRASHQWMNTQGGP CPG*CHRDSNQGIWTVPIPKSCLRNPLLQMNSL C*LHLSH*YFARLETCKTTV*QRKMILARKSFC EDGTS*HSPRPYY*KMHFHFDSDFPTREPGWAAL SPLASIFPIS*H*LPPVLPSPPSPLQGVPGAAI KTL*AV*RF*QTLTYMESQYKEQMNQSSSIKCF IN*NVLEILFLKPTSTFCRNLFQYLLNNFECVGP SKIYLIPQYCGTPPGASWWSSRFPLEAVSPSRS MTS*VWGVLWGVGQWPGLGLSQFFPFQECTNTS PER*WVAMPSASWAGEWRMAHPTGWLPTPGTLTG VTMVSGCPLPAKNSEL*ATPRGHLGFLCSTHRT ALNPGPTARL*APPKCQAVRSSLLL*GQTLSSF RANACNSSHSIAPDPVMGRVTGQGACPWRCAPRL QKPCGVEDCASGPTAGVARDE*LKVF*G*V*LY WLTLVTL*KHLTFYSQASLKYSEDRITVESNQK WWLEFHAPISTGKRSNLPWACRASPGGEIGVEMH FIL*VHVRYKFQTGSEGLDWPNIRPVFQGDQVL ATSQPVVTVQTGHVSHRCQHRASFPL*TSAVGS TCCPS*LWPPP*SIHLQGARQRRRNGKRSS*QDE SSPISSPSTSKQVAFHICHRNQRRDGVGSPLEN ASLPGPLHLSSLQCHNLSDLVLSMIL**KFYFF VHSANHVGEPVEQRETCASFTDCLLMTRLKRKPS GQELFLTH*SLLPQGK*FRRNQLLLFLKNYSFT LSS*QGMPVPIKVFSNLKSTLMGSQILCHCL*T CSCCLSLSLQRITSWNCMFLRLLGLHLNFSLPQP CFQPWHPSPN*FPVILVNLLCKCLVSLPLLKNS KHSCALFFCCCCD*QSEIPSPRLECSGAFSAHC NLQPPRFKRFSCFSLPSSWDDRHSPICLGNFCIF KYIQDFTMLARLVSNSRPQVVCLPQPPKVLGLQ A*ATGPCLYFLSATNPATS*GFSS*NRLGVLVI SHNQDATPWGTTSPWMPSSLGLGWSQGLYTVF*I CMPLVCIAGQEL*CFA*PWFRNML*QFLV*SKL EEPAIIPLSCQGTPQYSPSQLKWYEASSFQKHS SLASDVTQNLGTRC*IK*ALKI*GTL*ERFVKHL LSAMMSITAGRRGSRL*SQHFGRPRWEDDKVRS SRPAWPTC*NLTSTKNTKISWAWWHMPVIPATW EAEAGESLEPGRRRLQ*AKTVPVHSSLGDSARLR LNN

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR AGWAAGSRLQRWGLALSPRLVCSGATTAHGSLS HPDVSDLVPTSASRVDTSENRNDDSGMQTLAVL CIRRWLFTLPVTEEVSPRWRCSEPGAGCLQAYGE QLAWAGPAVPGLHR*LAGGSRIRLPTCGSSGPP SAACWCWPMPGAGPLSIPCRMSWSTMSTNGIPR GRPGTTSTTWT*AT*RGYVVPSWVGPSHPRELCL PRT*SCLQASMHGNNGHSVPPSKRSETRAPVAP AG*GPAGWRGSAGEKVGATLESLGGFGVGTTLT RQVIETF*VPVSISTKITIPLAMNDGED*VEWRA EHPADCKSTCTGGEAAPFP*HAGPSANTRSSHC YSASRKHPFSVSELS*FCHLIVVKYT*QKCNNL SNLLLFSFSFFFFFFFFWRWSLALSPRLECSGGI LAHCNLCLLGSSNSPASVSPVAKTTGMCHHAQL IFVFLVEMGFCHVGQPGLKRLTC*SAFLGLAKC WDYRREPPCPATIFKCTVL*H*VHSQCCSATTTI CPPNSFSARKTETLSPLTPIL*PLVSPTLLSVS MNLTPRDLIQVDHNSIYFLGELCFLLRKKHSQV R*LTPVISTLWEAEAGGSPEVRSLRPTWPTW*NP VSTKKTKLSWAWWQAPIIPATIEAEAGELLEPR RRRLQ*AEIAPLHSSLGNKSETPPPGKKKKTTH THTN*YYTFCRFQK*TQFPGRGSRGCFSCFKS*V GQIVEAGEWGKWLITPLIPALWEAKVGKSLEPG SSRPVWPT*QDPISTKENKS*V*APGASPGVER SVAYKV*GAPVAL*GDCCRGPSPDMAASPGGARS CRPSGLWKPSLTGSASTPMRTSAWRCRRRTCSH AVAACVGTGESGCASTAGLVLRDPGPQLLSGGD FEDFLGLLRVRSVSGRLRESGRQGLARLLLPGRC LNQGWPGRAV*GVASQQNSQFQKMWWNVWPLVW FVSSHVPFSCLGNQFGAFPYV*LAGMAPP*QQG GGTELSAPAACSLALVPQ*RCSLAAADPKCLRA* KNAWASPLTS*MGITGDSHFRELVVFLSTLGKF SVCSVENQCSTRKGTRSLVWYGHEQESLLSSPG SLWAGVGLAVIPCKLCLSRERPLEGLGSGRSART TCCSWGWVHEWPRALG*HTVVWRAGGASLRGFH *RLSFQL*WWLSC*SLELLDKKRPGFWWPL*IP CR*VCPLGHFLARWIV*AINHHGFICLYKLGVET EGLLRGASQVHRPLAGPAWRPCCRHEACLAPGL LRGGCRLTPLSPASHQGADRTPSLPVSTTSTAP GPHARGREIPPSVARSVSLATARPTNRTSTTVRG LGPGHGARGGWGAAASLLTLQRRGQG*GALGSR NSRIEEGE*CPLARRSSTLSTFCLFHPELSLSP SKLEGTKALLLQGSGVSWSLEVSWPLPGPRAHCS VLPFLSFQPQPCP*FFRGAFLELPWQGKSP*MQ SSPGGHQKSVTG*PWRATRHGHCPPQPRARFIF QDTIPTASPIARRTSWPRSTKTAPWRELSLCIRT SCSTSQVRADG*WQ*RVGVGRGAWVPGWFMLTN RAGLGRQVRGWGRGLCGAFHIGSLLCGPAWHLT VFVQVLCQHTQFQRPLPEPLG*FLPIH*RPAHTC SLWERPPPWWLATPALAFLALHCFTSMFLL*WQ AVRVCTG*HIFFIFLFAKA*IVPHTSG*THRED LALGSAIGTPTKESGQSPSPSPVLEIPCSR*TVF ADCIYPINILLDWKLVKQQFDKGR*FWQEKVSV KMALLSIVLALIIKRCISTLTVISPPESLVGQP *AP*PVSFPSASISCHQSSLLLPHLFRVYLEQLL KLFKPCNAFDKPLLTWNPSIKNR*ISHLALNVL SIRMY*KFYF*SQQAHFVGIYFNIYSTTLSVLG LQKYI*FPSIVGPPLEPPGGAAGSLWKLFLLPGA *PAECGGCCGAWDSGPVSVSASSSLFRSVPTRH RRDDGWPCHPHPGLGSGEWHTLLAGCQLLEH*L G*QW*VAAPFLPRTVNCEPHPVAISAFSVLPTAQ P*TLDLRPGCELLLSARQSGVPFCCEGRL*AAS EPTPATVPTASRQIL*WEG*PGRGLARGGVPHG SRSLVVLRTVLVGQQQEWPGMSDLRSFKDESDYI G*PLSHFKSTLLFIPRLL*NTQRTGSLWNRIRS GGWNSTHRSVLGKDLICRGPVVPVLGARSG*KC ILFFKFT*DTSFRQGLKDWIGQTSDLSSKETKSW LHPSLWLQCRQAM*ATAASTERPSPCRLVP*GV PAAPADCGPLRDPSISREQDRDAGMESGVPNRM KVPPSVPPVPPSK*LSTFVTEIRGETVLGALWRT PVSQAPCIYRVCNVTTSLILCSA*FFNRSFIFS CTLLIMWVSQWNSGRPVLVLQIASL*RGSKGNQ VVRSCF*PTDLYYHKENSLGETSFYCF*KITASP CQVNKECLCQ*KFSPT*SLL*WDLRSFVTAYRL VAAVSLCPCRESRPGTACSCDSWDFILTSRCPS HVFNHGIPPPISSLSSSSTFSVSAW*ACPCLRTQ NIAVLYFFVVVVTDRVRFRLPGWSAVAPSQLTA TCSLLDSSDSPASAFRVAGMTGTHQYAWVIFVF LSTYRISPCWPG*FQTPGLRWSACLSLPKCWDYR REPLGPACIFYQPQIQQQAEDSAHKTGLVSW*S HITKMLPRGEPHPPGCPPALVWAGVRACIQYFE FVCHWFALLVRNSSALHSPGLETCYSSSWYRAN* KNQQSFHCPAKVHLSTPLPN*SGMRLALSKSIQ VWLLM*LRI*EPDARSNKL*KSEEHCRKGLLSI S*VP**A*QPAVVAHACNPSTLGGQGGRMTRSGV QDQPGQHAETSPLLKIQKLAGHGGTCL*SQLLG RLRQENRLNPGGGGCSEPRQCQCTPASVTAQGS VSII

Sequence CTSB.cAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGTGTCTTCAGGCCTATGGAGAGCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAG ATGATTGGCAGGTGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCT CTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTT GAAGAGGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAA CAATGGCCACAGTGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCT GCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCC TGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAG CACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGG ACAAGCACTACGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGT GTATTCGGACTTCCTGCTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAG AATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCG AATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGG TAGAAATGCATTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGA CCAAGTCCTGGCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCC GTAGGGAGTACCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCT AACAGGATGAAAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCC TTTGGAGAACGCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTT TATTTTTTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAA GGAAACCAAGTGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTA CAGCTTCACCCTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGC CTATAGACTTGTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGC TGCCCCAGCCATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCT TAAGAACTCAAAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCA GCTCACTGCAACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATT TTTGTATTTTTAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTT GGGATTACAGGCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTC TTGGTGATCTCACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAG TATTTTGAATTTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATA GAGCAAACTAGAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAA AAGCATTCAAGTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTG TTAAGCATCTCTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAA GGTCAGGAGTTCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCA GCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCG CAAGGCTCCGTCTCAATAATTAAAAAAAAAAAAAAAAAAAAAAAGGCCGGGCGCAGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGG CAGATCACCTGAGGTCAGGAGTTTTGAGATCAGCCTTGGCAACACGGTGAAACCCCATCTCTACTAAAAATACAAAATTAGCCAAGCATGCTGGCACATG CCTGTAATCCCAGCTACTCGGGAGGCTGAGGTACGAGAATCGCTTGAACCTGGGAGGCAGAGGATGCAGTGAGCCGAGATCACGCCATTGCACTCCAGCC TGGGGGACAAGAGTGAATCTGTGTCTCACCAAAAAAAAAAAGAAAAAGAAAGATGCTTAACAAAGGTTACCATAAGCCACAAATTCATAACCACTTATCC TTCCAGTTTCAAGTAGAATATATTCATAACCTCAATAAAGTTCTCCCTGCTCCCAAACTGAGTTGATTCATTCCATGCGCACTGTCTCCCTCACCTCCAT CGTATACGCACCTGCAGCTCCTTGCCTGCTTCTAGCATGGAGCCAGCGGGGATGTGCTGGTGAGTGACAAGGAATTGTGGGGCATGTCGACATCAGCTTA AGGCTTTGTTAGAAACACCTGCCTTGCAGTGTTTGTGACTGGGAAAGAGCTCAGTGTTTTAATACACCTCAGATCACCACATTGATCATAACTTTGTCAC CTCAGTTCAATAGTTTCTCCTGGATTCTTGTCCAAGTGAGATGACAGGTGGACGTAACAATGCCTTTTCAGTCTTTACCTAGCCTCTCCAGAGAATCACT CCAGAATAGAAATTAGAGTATAGGTAGGCAGTCCAACCTCTGCATTT

CTSB.F6 (forward primer)
CTSB.R2 (reverse primer)
Alternative donor

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC AQAGLQALGCSAGCLQAYGEQLAWAGPAVPGLHR *LAGGSRIRLPTCGSSGPPSAACWCWPMPGAGP LSIPCRMSWSTMSTNGIPRGRPGTTSTTWT*AT *RGYVVPSWVGPSHPRELCLPRT*SCLQASMHGN NGHSVPPSKRSETRAPVAPAGPSGLWKPSLTGS ASTPMRTSAWRCRRRTCSHAVAACVGTAVMVAI LLKLGTSGQEKAWFLVASMNPM*GADRTPSLPVS TTSTAPGPHARGREIPPSVARSVSLATARPTNR TSTTDTIPTASPIARRTSWPRSTKTAPWRELSL CIRTSCSTSQECTNTSPER*WVAMPSASWAGEWR MAHPTGWLPTPGTLTGVTMASLKYSEDRITVES NQKWWLEFHAPISTGKRSNLPWACRASPGGEIG VEMHFIL*VHVRYKFQTGSEGLDWPNIRPVFQGD QVLATSQPVVTVQTGHVSHRCQHRASFPL*TSA VGSTCCPS*LWPPP*SIHLQGARQRRRNGKRSS *QDESSPISSPSTSKQVAFHICHRNQRRDGVGSP LENASLPGPLHLSSLQCHNLSDLVLSMIL**KF YFFVHSANHVGEPVEQRETCASFTDCLLMTRLK RKPSGQELFLTH*SLLPQGK*FRRNQLLLFLKNY SFTLSS*QGMPVPIKVFSNLKSTLMGSQILCHC L*TCSCCLSLSLQRITSWNCMFLRLLGLHLNFS LPQPCFQPWHPSPN*FPVILVNLLCKCLVSLPLL KNSKHSCALFFCCCCD*QSEIPSPRLECSGAFS AHCNLQPPRFKRFSCFSLPSSWDDRHSPICLGN FCIFKYIQDFTMLARLVSNSRPQVVCLPQPPKVL GLQA*ATGPCLYFLSATNPATS*GFSS*NRLGV LVISHNQDATPWGTTSPWMPSSLGLGWSQGLYT VF*ICMPLVCIAGQEL*CFA*PWFRNML*QFLV* SKLEEPAIIPLSCQGTPQYSPSQLKWYEASSFQ KHSSLASDVTQNLGTRC*IK*ALKI*GTL*ERF VKHLLSAMMSITAGRRGSRL*SQHFGRPRWEDDK VRSSRPAWPTC*NLTSTKNTKISWAWWHMPVIP ATWEAEAGESLEPGRRRLQ*AKTVPVHSSLGDS ARLRLNN*KKKKKKKGRAQWLKPVIPALWEAEAG RSPEVRSFEISLGNTVKPHLY*KYKISQACWHM PVIPATREAEVRESLEPGRQRMQ*AEITPLHSS LGDKSESVSHQKKKEKERCLTKVTISHKFITTYP SSFK*NIFITSIKFSLLPN*VDSFHAHCLPHLH RIRTCSSLPASSMEPAGMCW*VTRNCGACRHQL KALLETPALQCL*LGKSSVF*YTSDHHIDHNFVT SVQ*FLLDSCPSEMTGGRNNAFSVFT*PLQRIT PE*KLEYR*AVQPLH

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA RRLGCRLSAAALGVFRPMESSLRGLGLQYLVCI DDWQVDLGSGFQHVAALGLPLLPAGVGQCPEQAL FPSPVG*AGQLCQQTEYHVAGRAQLLQRGHELL EEAMWYLPGWAQATPESYVYRGPEAACKLRCTG TMATVSHHQRDQRPGLLWLLLGLRGCGSHL*PDL HPHQCARQRGGVGGGPAHMLWQHVWGRL*WWLS C*SLELLDKKRPGFWWPL*IPCRVQTVLHPSL* APRQRLPAPMHGGGRYPQV*QDL*AWLQPDLQTG QALRIQFLQRLQ*REGHHGRDLQKRPRGGSFLC VFGLPALQVRSVPTRHRRDDGWPCHPHPGLGSG EWHTLLAGCQLLEH*LG*QWLL*NTQRTGSLWNR IRSGGWNSTHRSVLGKDLICRGPVVPVLGARSG *KCILFFKFT*DTSFRQGLKDWIGQTSDLSSKE TKSWLHPSLWLQCRQAM*ATAASTERPSPCRLVP *GVPAAPADCGPLRDPSISREQDRDAGMESGVP NRMKVPPSVPPVPPSK*LSTFVTEIRGETVLGA LWRTPVSQAPCIYRVCNVTTSLILCSA*FFNRSF IFSCTLLIMWVSQWNSGRPVLVLQIASL*RGSK GNQVVRSCF*PTDLYYHKENSLGETSFYCF*KI TASPCQVNKECLCQ*KFSPT*SLL*WDLRSFVTA YRLVAAVSLCPCRESRPGTACSCDSWDFILTSR CPSHVFNHGIPPPISSLSSSSTFSVSAW*ACPC LRTQNIAVLYFFVVVVTDRVRFRLPGWSAVAPSQ LTATCSLLDSSDSPASAFRVAGMTGTHQYAWVI FVFLSTYRISPCWPG*FQTPGLRWSACLSLPKC WDYRREPLGPACIFYQPQIQQQAEDSAHKTGLVS W*SHITKMLPRGEPHPPGCPPALVWAGVRACIQ YFEFVCHWFALLVRNSSALHSPGLETCYSSSWY RAN*KNQQSFHCPAKVHLSTPLPN*SGMRLALSK SIQVWLLM*LRI*EPDARSNKL*KSEEHCRKGL LSIS*VP**A*QPAVVAHACNPSTLGGQGGRMT RSGVQDQPGQHAETSPLLKIQKLAGHGGTCL*SQ LLGRLRQENRLNPGGGGCSEPRQCQCTPASVTA QGSVSIIKKKKKKKKAGRSGSSL*SQHFGRLRR ADHLRSGVLRSALATR*NPISTKNTKLAKHAGTC L*SQLLGRLRYENRLNLGGRGCSEPRSRHCTPA WGTRVNLCLTKKKKKKKDA*QRLP*ATNS*PLI LPVSSRIYS*PQ*SSPCSQTELIHSMRTVSLTSI VYAPAAPCLLLAWSQRGCAGE*QGIVGHVDISL RLC*KHLPCSVCDWERAQCFNTPQITTLIITLS PQFNSFSWILVQVR*QVDVTMPFQSLPSLSRESL QNRN*SIGRQSNLCI

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR AGWAAGSRLQRWVSSGLWRAACVGWACSTWFA* MIGRWI*DPASNMWQLWASLCCLLVLANARSRP SFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYL KRLCGTFLGGPKPPQRVMFTEDLKLPASFDARE QWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRI CIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYP AEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCE HHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQ DKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSV YSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVE NGTPYWLVANSWNTDWGDNGFFKILRGQDHCGI ESEVVAGIPRTDQYWEKI*SAVGLSCQSWGRDRG RNAFYSLSSRKIQVSDRV*RTGLAKHQTCLPRR PSPGYIPACGYSADRPCEPPLPAQSVLPPVD*C RREYLLPQLTVAPSVIHPSPGSKTETQEWKAEFL TG*KFPHQFPQYLQASSFPHLSQKSEERRCWEP FGERQSPRPPASIEFAMSQPL*SCAQHDSLIEV LFFRALC*SCG*ASGTAGDLC*FYRLPPYDAAQK ETKWSGVVSDPLISTTTRKIV*EKPAFTVFEKL QLHPVKLTRNACANKSFLQLEVYSDGISDPLSL PIDL*LLSLFVPAENHVLELHVLATLGTSS*LLA APAMFSTMASLPQLVPCHPRQPSL*VPGKLALA *ELKT*LCSIFLLLL*LTE*DSVSQAGVQWRLL SSLQPAAS*IQAILLLQPSE*LG*QALTNMPG*F LYF*VHTGFHHVGQASFKLPASGGLPASASQSV GITGVSHWALPVFFISHKSSNKLRIQLIKQAWC LGDLT*PRCYPVGNHIPLDALQPWFGLESGPVYS ILNLYATGLHCWSGTLVLCIALV*KHVIAVLGI EQTRRTSNHSTVLPRYTSVLPFPTEVV*G*LFP KAFKFGF*CDSEFRNQMLDQISSENLRNIVGKVC *ASLKCHDEHNSRPSWLTPVIPALWEAKVGG*Q GQEFKTSLANMLKPHLY*KYKN*LGMVAHACNP SYLGG*GRRIA*TREAEVAVSQDSASALQPR*QR KAPSQ*LKKKKKKKRPGAVAQACNPSTLGG*GG QIT*GQEF*DQPWQHGETPSLLKIQN*PSMLAH ACNPSYSGG*GTRIA*TWEAEDAVSRDHAIALQP GGQE*ICVSPKKKRKRKMLNKGYHKPQIHNHLS FQFQVEYIHNLNKVLPAPKLS*FIPCALSPSPP SYTHLQLLACF*HGASGDVLVSDKELWGMSTSA* GFVRNTCLAVFVTGKELSVLIHLRSPH*S*LCH LSSIVSPGFLSK*DDRWT*QCLFSLYLASPENH SRIEIRV*VGSPTSAF

Sequence CTSB.bAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGCTGGTGTGCAGTGGTGCGACCACGGCTCACGGCAGCCTCAGCCACCCAGATGTAAGCGATCT GGTTCCCACCTCAGCCTCCCGAGTAGTGTCTTCAGGCCTATGGAGAGCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAGATGATTGGCAGG TGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCCATCCCC TGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATG TGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAG TGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCA ATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCCTGCTGAAGCTTG GAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAAC GGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACG GATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGACTT CCTGCTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCC TACTGGCTGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGTGG TGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGGTAGAAATGCATT TTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTGGC TACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTACC TGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGAAA GTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAACGC CAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCGTG CACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAGTG GTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACCCT GTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTTGT AGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCCAT GTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCAAA ACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCAAC CTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTTTA AGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGC GTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCTCA CATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAATTT GTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGA AGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAAGT TTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCTCT TAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGTTC AAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAG GCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCGTC TCAATAATTAAAAAAAAAAAAAAAAAAAAAAAGGCCGGGCGCAGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGA GGTCAGGAGTTTTGAGATCAGCCTTGGCAACACGGTGAAACCCCATCTCTACTAAAAATACAAAATTAGCCAAGCATGCTGGCACATGCCTGTAATCCCA GCTACTCGGGAGGCTGAGGTACGAGAATCGCTTGAACCTGGGAGGCAGAGGATGCAGTGAGCCGAGATCACGCCATTGCACTCCAGCCTGGGGGACAAGA GTGAATCTGTGTCTCACCAAAAAAAAAAAGAAAAAGAAAGATGCTTAACAAAGGTTACCATAAGCCACAAATTCATAACCACTTATCCTTCCAGTTTCAA GTAGAATATATTCATAACCTCAATAAAGTTCTCCCTGCTCCCAAACTGAGTTGATTCATTCCATGCGCACTGTCTCCCTCACCTCCATCGTATACGCACC TGCAGCTCCTTGCCTGCTTCTAGCATGGAGCCAGCGGGGATGTGCTGGTGAGTGACAAGGAATTGTGGGGCATGTCGACATCAGCTTAAGGCTTTGTTAG AAACACCTGCCTTGCAGTGTTTGTGACTGGGAAAGAGCTCAGTGTTTTAATACACCTCAGATCACCACATTGATCATAACTTTGTCACCTCAGTTCAATA GTTTCTCCTGGATTCTTGTCCAAGTGAGATGACAGGTGGACGTAACAATGCCTTTTCAGTCTTTACCTAGCCTCTCCAGAGAATCACTCCAGAATAGAAA TTAGAGTATAGGTAGGCAGTCCAACCTCTGCATTT

CTSB.F6 (forward primer)
CTSB.R2 (reverse primer)
Alternative donor

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC AQAGLQALGCSAGLVCSGATTAHGSLSHPDVSDL VPTSASRVVSSGLWRAACVGWACSTWFA*MIGR WI*DPASNMWQLWASLCCLLVLANARSRPSFHP LSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLC GTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQ CPTIKEIRDQGSCGSCWAFGAVEAISDRICIHT NAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAW NFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVN GSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHY GYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDF LLYKSGVYQHVTGEMMGGHAIRILGWGVENGTP YWLVANSWNTDWGDNGFFKILRGQDHCGIESEV VAGIPRTDQYWEKI*SAVGLSCQSWGRDRGRNAF YSLSSRKIQVSDRV*RTGLAKHQTCLPRRPSPG YIPACGYSADRPCEPPLPAQSVLPPVD*CRREY LLPQLTVAPSVIHPSPGSKTETQEWKAEFLTG*K FPHQFPQYLQASSFPHLSQKSEERRCWEPFGER QSPRPPASIEFAMSQPL*SCAQHDSLIEVLFFR ALC*SCG*ASGTAGDLC*FYRLPPYDAAQKETKW SGVVSDPLISTTTRKIV*EKPAFTVFEKLQLHP VKLTRNACANKSFLQLEVYSDGISDPLSLPIDL *LLSLFVPAENHVLELHVLATLGTSS*LLAAPAM FSTMASLPQLVPCHPRQPSL*VPGKLALA*ELK T*LCSIFLLLL*LTE*DSVSQAGVQWRLLSSLQ PAAS*IQAILLLQPSE*LG*QALTNMPG*FLYF* VHTGFHHVGQASFKLPASGGLPASASQSVGITG VSHWALPVFFISHKSSNKLRIQLIKQAWCLGDL T*PRCYPVGNHIPLDALQPWFGLESGPVYSILNL YATGLHCWSGTLVLCIALV*KHVIAVLGIEQTR RTSNHSTVLPRYTSVLPFPTEVV*G*LFPKAFK FGF*CDSEFRNQMLDQISSENLRNIVGKVC*ASL KCHDEHNSRPSWLTPVIPALWEAKVGG*QGQEF KTSLANMLKPHLY*KYKN*LGMVAHACNPSYLG G*GRRIA*TREAEVAVSQDSASALQPR*QRKAPS Q*LKKKKKKKRPGAVAQACNPSTLGG*GGQIT* GQEF*DQPWQHGETPSLLKIQN*PSMLAHACNP SYSGG*GTRIA*TWEAEDAVSRDHAIALQPGGQE *ICVSPKKKRKRKMLNKGYHKPQIHNHLSFQFQ VEYIHNLNKVLPAPKLS*FIPCALSPSPPSYTH LQLLACF*HGASGDVLVSDKELWGMSTSA*GFVR NTCLAVFVTGKELSVLIHLRSPH*S*LCHLSSI VSPGFLSK*DDRWT*QCLFSLYLASPENHSRIE IRV*VGSPTSAF

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA RRLGCRLSAAALGWCAVVRPRLTAASATQM*AI WFPPQPPE*CLQAYGEQLAWAGPAVPGLHR*LAG GSRIRLPTCGSSGPPSAACWCWPMPGAGPLSIP CRMSWSTMSTNGIPRGRPGTTSTTWT*AT*RGY VVPSWVGPSHPRELCLPRT*SCLQASMHGNNGHS VPPSKRSETRAPVAPAGPSGLWKPSLTGSASTP MRTSAWRCRRRTCSHAVAACVGTAVMVAILLKL GTSGQEKAWFLVASMNPM*GADRTPSLPVSTTST APGPHARGREIPPSVARSVSLATARPTNRTSTT DTIPTASPIARRTSWPRSTKTAPWRELSLCIRT SCSTSQECTNTSPER*WVAMPSASWAGEWRMAHP TGWLPTPGTLTGVTMASLKYSEDRITVESNQKW WLEFHAPISTGKRSNLPWACRASPGGEIGVEMH FIL*VHVRYKFQTGSEGLDWPNIRPVFQGDQVLA TSQPVVTVQTGHVSHRCQHRASFPL*TSAVGST CCPS*LWPPP*SIHLQGARQRRRNGKRSS*QDE SSPISSPSTSKQVAFHICHRNQRRDGVGSPLENA SLPGPLHLSSLQCHNLSDLVLSMIL**KFYFFV HSANHVGEPVEQRETCASFTDCLLMTRLKRKPS GQELFLTH*SLLPQGK*FRRNQLLLFLKNYSFTL SS*QGMPVPIKVFSNLKSTLMGSQILCHCL*TC SCCLSLSLQRITSWNCMFLRLLGLHLNFSLPQP CFQPWHPSPN*FPVILVNLLCKCLVSLPLLKNSK HSCALFFCCCCD*QSEIPSPRLECSGAFSAHCN LQPPRFKRFSCFSLPSSWDDRHSPICLGNFCIF KYIQDFTMLARLVSNSRPQVVCLPQPPKVLGLQA *ATGPCLYFLSATNPATS*GFSS*NRLGVLVIS HNQDATPWGTTSPWMPSSLGLGWSQGLYTVF*I CMPLVCIAGQEL*CFA*PWFRNML*QFLV*SKLE EPAIIPLSCQGTPQYSPSQLKWYEASSFQKHSS LASDVTQNLGTRC*IK*ALKI*GTL*ERFVKHL LSAMMSITAGRRGSRL*SQHFGRPRWEDDKVRSS RPAWPTC*NLTSTKNTKISWAWWHMPVIPATWE AEAGESLEPGRRRLQ*AKTVPVHSSLGDSARLR LNN*KKKKKKKGRAQWLKPVIPALWEAEAGRSPE VRSFEISLGNTVKPHLY*KYKISQACWHMPVIP ATREAEVRESLEPGRQRMQ*AEITPLHSSLGDK SESVSHQKKKEKERCLTKVTISHKFITTYPSSFK *NIFITSIKFSLLPN*VDSFHAHCLPHLHRIRT CSSLPASSMEPAGMCW*VTRNCGACRHQLKALL ETPALQCL*LGKSSVF*YTSDHHIDHNFVTSVQ* FLLDSCPSEMTGGRNNAFSVFT*PLQRITPE*K LEYR*AVQPLH

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR AGWAAGSRLQRWAGVQWCDHGSRQPQPPRCKRS GSHLSLPSSVFRPMESSLRGLGLQYLVCIDDWQ VDLGSGFQHVAALGLPLLPAGVGQCPEQALFPSP VG*AGQLCQQTEYHVAGRAQLLQRGHELLEEAM WYLPGWAQATPESYVYRGPEAACKLRCTGTMAT VSHHQRDQRPGLLWLLLGLRGCGSHL*PDLHPHQ CARQRGGVGGGPAHMLWQHVWGRL*WWLSC*SL ELLDKKRPGFWWPL*IPCRVQTVLHPSL*APRQ RLPAPMHGGGRYPQV*QDL*AWLQPDLQTGQALR IQFLQRLQ*REGHHGRDLQKRPRGGSFLCVFGL PALQVRSVPTRHRRDDGWPCHPHPGLGSGEWHT LLAGCQLLEH*LG*QWLL*NTQRTGSLWNRIRSG GWNSTHRSVLGKDLICRGPVVPVLGARSG*KCI LFFKFT*DTSFRQGLKDWIGQTSDLSSKETKSW LHPSLWLQCRQAM*ATAASTERPSPCRLVP*GVP AAPADCGPLRDPSISREQDRDAGMESGVPNRMK VPPSVPPVPPSK*LSTFVTEIRGETVLGALWRT PVSQAPCIYRVCNVTTSLILCSA*FFNRSFIFSC TLLIMWVSQWNSGRPVLVLQIASL*RGSKGNQV VRSCF*PTDLYYHKENSLGETSFYCF*KITASP CQVNKECLCQ*KFSPT*SLL*WDLRSFVTAYRLV AAVSLCPCRESRPGTACSCDSWDFILTSRCPSH VFNHGIPPPISSLSSSSTFSVSAW*ACPCLRTQ NIAVLYFFVVVVTDRVRFRLPGWSAVAPSQLTAT CSLLDSSDSPASAFRVAGMTGTHQYAWVIFVFL STYRISPCWPG*FQTPGLRWSACLSLPKCWDYR REPLGPACIFYQPQIQQQAEDSAHKTGLVSW*SH ITKMLPRGEPHPPGCPPALVWAGVRACIQYFEF VCHWFALLVRNSSALHSPGLETCYSSSWYRAN* KNQQSFHCPAKVHLSTPLPN*SGMRLALSKSIQV WLLM*LRI*EPDARSNKL*KSEEHCRKGLLSIS *VP**A*QPAVVAHACNPSTLGGQGGRMTRSGV QDQPGQHAETSPLLKIQKLAGHGGTCL*SQLLGR LRQENRLNPGGGGCSEPRQCQCTPASVTAQGSV SIIKKKKKKKKAGRSGSSL*SQHFGRLRRADHL RSGVLRSALATR*NPISTKNTKLAKHAGTCL*SQ LLGRLRYENRLNLGGRGCSEPRSRHCTPAWGTR VNLCLTKKKKKKKDA*QRLP*ATNS*PLILPVS SRIYS*PQ*SSPCSQTELIHSMRTVSLTSIVYAP AAPCLLLAWSQRGCAGE*QGIVGHVDISLRLC* KHLPCSVCDWERAQCFNTPQITTLIITLSPQFN SFSWILVQVR*QVDVTMPFQSLPSLSRESLQNRN *SIGRQSNLCI

Sequence CTSB.eAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGTGTCTTCAGGCCTATGGAGAGCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAG ATGATTGGCAGGTGGGCAGCACGGGGAAGGACCTGTGAGTGGCCAACCTGGTTCAGGTGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCT CCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCAC GTGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATG TTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCT CCTGCTGGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCAC ATGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTAT GAATCCCATGTAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGT GTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCAT GGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGACTTCCTGCTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAG ATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACTGGGGTGACA ATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTA ATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGGTAGAAATGCATTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTC TGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTGGCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGC CACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTACCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATC TCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGAAAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTT CCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAACGCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCAC AACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGA CCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAGTGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGG AAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACCCTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCC AACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTTGTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGA ACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCCATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCAT CCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCAAAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAG AGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCAACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTC CGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTTTAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAA CTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAAT CCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCTCACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGA TGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAATTTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTT TGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAG TACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAAGTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGA TCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCTCTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACG CCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAA AATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGC AGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCGTCTCAATAATTAAAAAAAAAAAAAAAAAAAAAAAGGCCGGGCGCA GTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGAGGTCAGGAGTTTTGAGATCAGCCTTGGCAACACGGTGAAACCC CATCTCTACTAAAAATACAAAATTAGCCAAGCATGCTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGTACGAGAATCGCTTGAACCTGGGA GGCAGAGGATGCAGTGAGCCGAGATCACGCCATTGCACTCCAGCCTGGGGGACAAGAGTGAATCTGTGTCTCACCAAAAAAAAAAAGAAAAAGAAAGATG CTTAACAAAGGTTACCATAAGCCACAAATTCATAACCACTTATCCTTCCAGTTTCAAGTAGAATATATTCATAACCTCAATAAAGTTCTCCCTGCTCCCA AACTGAGTTGATTCATTCCATGCGCACTGTCTCCCTCACCTCCATCGTATACGCACCTGCAGCTCCTTGCCTGCTTCTAGCATGGAGCCAGCGGGGATGT GCTGGTGAGTGACAAGGAATTGTGGGGCATGTCGACATCAGCTTAAGGCTTTGTTAGAAACACCTGCCTTGCAGTGTTTGTGACTGGGAAAGAGCTCAGT GTTTTAATACACCTCAGATCACCACATTGATCATAACTTTGTCACCTCAGTTCAATAGTTTCTCCTGGATTCTTGTCCAAGTGAGATGACAGGTGGACGT AACAATGCCTTTTCAGTCTTTACCTAGCCTCTCCAGAGAATCACTCCAGAATAGAAATTAGAGTATAGGTAGGCAGTCCAACCTCTGCATTT

CTSB.F6 (forward primer)
CTSB.R2 (reverse primer)
Alternative donor

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC AQAGLQALGCSAGCLQAYGEQLAWAGPAVPGLHR *LAGGQHGEGPVSGQPGSGGSRIRLPTCGSSGP PSAACWCWPMPGAGPLSIPCRMSWSTMSTNGIP RGRPGTTSTTWT*AT*RGYVVPSWVGPSHPRELC LPRT*SCLQASMHGNNGHSVPPSKRSETRAPVA PAGPSGLWKPSLTGSASTPMRTSAWRCRRRTCS HAVAACVGTAVMVAILLKLGTSGQEKAWFLVASM NPM*GADRTPSLPVSTTSTAPGPHARGREIPPS VARSVSLATARPTNRTSTTDTIPTASPIARRTS WPRSTKTAPWRELSLCIRTSCSTSQECTNTSPER *WVAMPSASWAGEWRMAHPTGWLPTPGTLTGVT MASLKYSEDRITVESNQKWWLEFHAPISTGKRS NLPWACRASPGGEIGVEMHFIL*VHVRYKFQTGS EGLDWPNIRPVFQGDQVLATSQPVVTVQTGHVS HRCQHRASFPL*TSAVGSTCCPS*LWPPP*SIH LQGARQRRRNGKRSS*QDESSPISSPSTSKQVAF HICHRNQRRDGVGSPLENASLPGPLHLSSLQCH NLSDLVLSMIL**KFYFFVHSANHVGEPVEQRE TCASFTDCLLMTRLKRKPSGQELFLTH*SLLPQG K*FRRNQLLLFLKNYSFTLSS*QGMPVPIKVFS NLKSTLMGSQILCHCL*TCSCCLSLSLQRITSW NCMFLRLLGLHLNFSLPQPCFQPWHPSPN*FPVI LVNLLCKCLVSLPLLKNSKHSCALFFCCCCD*Q SEIPSPRLECSGAFSAHCNLQPPRFKRFSCFSL PSSWDDRHSPICLGNFCIFKYIQDFTMLARLVSN SRPQVVCLPQPPKVLGLQA*ATGPCLYFLSATN PATS*GFSS*NRLGVLVISHNQDATPWGTTSPW MPSSLGLGWSQGLYTVF*ICMPLVCIAGQEL*CF A*PWFRNML*QFLV*SKLEEPAIIPLSCQGTPQ YSPSQLKWYEASSFQKHSSLASDVTQNLGTRC* IK*ALKI*GTL*ERFVKHLLSAMMSITAGRRGSR L*SQHFGRPRWEDDKVRSSRPAWPTC*NLTSTK NTKISWAWWHMPVIPATWEAEAGESLEPGRRRL Q*AKTVPVHSSLGDSARLRLNN*KKKKKKKGRAQ WLKPVIPALWEAEAGRSPEVRSFEISLGNTVKP HLY*KYKISQACWHMPVIPATREAEVRESLEPG RQRMQ*AEITPLHSSLGDKSESVSHQKKKEKERC LTKVTISHKFITTYPSSFK*NIFITSIKFSLLP N*VDSFHAHCLPHLHRIRTCSSLPASSMEPAGM CW*VTRNCGACRHQLKALLETPALQCL*LGKSSV F*YTSDHHIDHNFVTSVQ*FLLDSCPSEMTGGR NNAFSVFT*PLQRITPE*KLEYR*AVQPLH

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA RRLGCRLSAAALGVFRPMESSLRGLGLQYLVCI DDWQVGSTGKDL*VANLVQVDLGSGFQHVAALGL PLLPAGVGQCPEQALFPSPVG*AGQLCQQTEYH VAGRAQLLQRGHELLEEAMWYLPGWAQATPESY VYRGPEAACKLRCTGTMATVSHHQRDQRPGLLWL LLGLRGCGSHL*PDLHPHQCARQRGGVGGGPAH MLWQHVWGRL*WWLSC*SLELLDKKRPGFWWPL *IPCRVQTVLHPSL*APRQRLPAPMHGGGRYPQV *QDL*AWLQPDLQTGQALRIQFLQRLQ*REGHH GRDLQKRPRGGSFLCVFGLPALQVRSVPTRHRR DDGWPCHPHPGLGSGEWHTLLAGCQLLEH*LG*Q WLL*NTQRTGSLWNRIRSGGWNSTHRSVLGKDL ICRGPVVPVLGARSG*KCILFFKFT*DTSFRQG LKDWIGQTSDLSSKETKSWLHPSLWLQCRQAM*A TAASTERPSPCRLVP*GVPAAPADCGPLRDPSI SREQDRDAGMESGVPNRMKVPPSVPPVPPSK*L STFVTEIRGETVLGALWRTPVSQAPCIYRVCNVT TSLILCSA*FFNRSFIFSCTLLIMWVSQWNSGR PVLVLQIASL*RGSKGNQVVRSCF*PTDLYYHK ENSLGETSFYCF*KITASPCQVNKECLCQ*KFSP T*SLL*WDLRSFVTAYRLVAAVSLCPCRESRPG TACSCDSWDFILTSRCPSHVFNHGIPPPISSLS SSSTFSVSAW*ACPCLRTQNIAVLYFFVVVVTDR VRFRLPGWSAVAPSQLTATCSLLDSSDSPASAF RVAGMTGTHQYAWVIFVFLSTYRISPCWPG*FQ TPGLRWSACLSLPKCWDYRREPLGPACIFYQPQI QQQAEDSAHKTGLVSW*SHITKMLPRGEPHPPG CPPALVWAGVRACIQYFEFVCHWFALLVRNSSA LHSPGLETCYSSSWYRAN*KNQQSFHCPAKVHLS TPLPN*SGMRLALSKSIQVWLLM*LRI*EPDAR SNKL*KSEEHCRKGLLSIS*VP**A*QPAVVAH ACNPSTLGGQGGRMTRSGVQDQPGQHAETSPLLK IQKLAGHGGTCL*SQLLGRLRQENRLNPGGGGC SEPRQCQCTPASVTAQGSVSIIKKKKKKKKAGR SGSSL*SQHFGRLRRADHLRSGVLRSALATR*NP ISTKNTKLAKHAGTCL*SQLLGRLRYENRLNLG GRGCSEPRSRHCTPAWGTRVNLCLTKKKKKKKD A*QRLP*ATNS*PLILPVSSRIYS*PQ*SSPCSQ TELIHSMRTVSLTSIVYAPAAPCLLLAWSQRGC AGE*QGIVGHVDISLRLC*KHLPCSVCDWERAQ CFNTPQITTLIITLSPQFNSFSWILVQVR*QVDV TMPFQSLPSLSRESLQNRN*SIGRQSNLCI

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR AGWAAGSRLQRWVSSGLWRAACVGWACSTWFA* MIGRWAARGRTCEWPTWFRWI*DPASNMWQLWA SLCCLLVLANARSRPSFHPLSDELVNYVNKRNTT WQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVM FTEDLKLPASFDAREQWPQCPTIKEIRDQGSCG SCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLT CCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLY ESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPK CSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIM AEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGE MMGGHAIRILGWGVENGTPYWLVANSWNTDWGD NGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI* SAVGLSCQSWGRDRGRNAFYSLSSRKIQVSDRV *RTGLAKHQTCLPRRPSPGYIPACGYSADRPCE PPLPAQSVLPPVD*CRREYLLPQLTVAPSVIHPS PGSKTETQEWKAEFLTG*KFPHQFPQYLQASSF PHLSQKSEERRCWEPFGERQSPRPPASIEFAMS QPL*SCAQHDSLIEVLFFRALC*SCG*ASGTAGD LC*FYRLPPYDAAQKETKWSGVVSDPLISTTTR KIV*EKPAFTVFEKLQLHPVKLTRNACANKSFL QLEVYSDGISDPLSLPIDL*LLSLFVPAENHVLE LHVLATLGTSS*LLAAPAMFSTMASLPQLVPCH PRQPSL*VPGKLALA*ELKT*LCSIFLLLL*LT E*DSVSQAGVQWRLLSSLQPAAS*IQAILLLQPS E*LG*QALTNMPG*FLYF*VHTGFHHVGQASFK LPASGGLPASASQSVGITGVSHWALPVFFISHK SSNKLRIQLIKQAWCLGDLT*PRCYPVGNHIPLD ALQPWFGLESGPVYSILNLYATGLHCWSGTLVL CIALV*KHVIAVLGIEQTRRTSNHSTVLPRYTS VLPFPTEVV*G*LFPKAFKFGF*CDSEFRNQMLD QISSENLRNIVGKVC*ASLKCHDEHNSRPSWLT PVIPALWEAKVGG*QGQEFKTSLANMLKPHLY* KYKN*LGMVAHACNPSYLGG*GRRIA*TREAEVA VSQDSASALQPR*QRKAPSQ*LKKKKKKKRPGA VAQACNPSTLGG*GGQIT*GQEF*DQPWQHGET PSLLKIQN*PSMLAHACNPSYSGG*GTRIA*TWE AEDAVSRDHAIALQPGGQE*ICVSPKKKRKRKM LNKGYHKPQIHNHLSFQFQVEYIHNLNKVLPAP KLS*FIPCALSPSPPSYTHLQLLACF*HGASGDV LVSDKELWGMSTSA*GFVRNTCLAVFVTGKELS VLIHLRSPH*S*LCHLSSIVSPGFLSK*DDRWT *QCLFSLYLASPENHSRIEIRV*VGSPTSAF