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Sequences

Sequence CTSB.bAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG
CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGCTGGTGTGCAGTGGTGCGACCACGGCTCACGGCAGCCTCAGCCACCCAGATGTAAGCGATCT
GGTTCCCACCTCAGCCTCCCGAGTAGTGTCTTCAGGCCTATGGAGAGCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAGATGATTGGCAGG
TGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCCATCCCC
TGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATG
TGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAG
TGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCA
ATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCCTGCTGAAGCTTG
GAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAAC
GGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACG
GATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGACTT
CCTGCTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCC
TACTGGCTGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGTGG
TGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGGTAGAAATGCATT
TTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTGGC
TACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTACC
TGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGAAA
GTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAACGC
CAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCGTG
CACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAGTG
GTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACCCT
GTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTTGT
AGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCCAT
GTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCAAA
ACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCAAC
CTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTTTA
AGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGC
GTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCTCA
CATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAATTT
GTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGA
AGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAAGT
TTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCTCT
TAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGTTC
AAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAG
GCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCGTC
TCAATAATTAAAAAAAAAAAAAAAAAAAAAAAGGCCGGGCGCAGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGA
GGTCAGGAGTTTTGAGATCAGCCTTGGCAACACGGTGAAACCCCATCTCTACTAAAAATACAAAATTAGCCAAGCATGCTGGCACATGCCTGTAATCCCA
GCTACTCGGGAGGCTGAGGTACGAGAATCGCTTGAACCTGGGAGGCAGAGGATGCAGTGAGCCGAGATCACGCCATTGCACTCCAGCCTGGGGGACAAGA
GTGAATCTGTGTCTCACCAAAAAAAAAAAGAAAAAGAAAGATGCTTAACAAAGGTTACCATAAGCCACAAATTCATAACCACTTATCCTTCCAGTTTCAA
GTAGAATATATTCATAACCTCAATAAAGTTCTCCCTGCTCCCAAACTGAGTTGATTCATTCCATGCGCACTGTCTCCCTCACCTCCATCGTATACGCACC
TGCAGCTCCTTGCCTGCTTCTAGCATGGAGCCAGCGGGGATGTGCTGGTGAGTGACAAGGAATTGTGGGGCATGTCGACATCAGCTTAAGGCTTTGTTAG
AAACACCTGCCTTGCAGTGTTTGTGACTGGGAAAGAGCTCAGTGTTTTAATACACCTCAGATCACCACATTGATCATAACTTTGTCACCTCAGTTCAATA
GTTTCTCCTGGATTCTTGTCCAAGTGAGATGACAGGTGGACGTAACAATGCCTTTTCAGTCTTTACCTAGCCTCTCCAGAGAATCACTCCAGAATAGAAA
TTAGAGTATAGGTAGGCAGTCCAACCTCTGCATTT
  • CTSB.F2 (forward primer)
  • CTSB.R6 (reverse primer)
  • Single exon skipping

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC
AQAGLQALGCSAGLVCSGATTAHGSLSHPDVSDL
VPTSASRVVSSGLWRAACVGWACSTWFA*MIGR
WI*DPASNMWQLWASLCCLLVLANARSRPSFHP
LSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLC
GTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQ
CPTIKEIRDQGSCGSCWAFGAVEAISDRICIHT
NAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAW
NFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVN
GSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHY
GYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDF
LLYKSGVYQHVTGEMMGGHAIRILGWGVENGTP
YWLVANSWNTDWGDNGFFKILRGQDHCGIESEV
VAGIPRTDQYWEKI*SAVGLSCQSWGRDRGRNAF
YSLSSRKIQVSDRV*RTGLAKHQTCLPRRPSPG
YIPACGYSADRPCEPPLPAQSVLPPVD*CRREY
LLPQLTVAPSVIHPSPGSKTETQEWKAEFLTG*K
FPHQFPQYLQASSFPHLSQKSEERRCWEPFGER
QSPRPPASIEFAMSQPL*SCAQHDSLIEVLFFR
ALC*SCG*ASGTAGDLC*FYRLPPYDAAQKETKW
SGVVSDPLISTTTRKIV*EKPAFTVFEKLQLHP
VKLTRNACANKSFLQLEVYSDGISDPLSLPIDL
*LLSLFVPAENHVLELHVLATLGTSS*LLAAPAM
FSTMASLPQLVPCHPRQPSL*VPGKLALA*ELK
T*LCSIFLLLL*LTE*DSVSQAGVQWRLLSSLQ
PAAS*IQAILLLQPSE*LG*QALTNMPG*FLYF*
VHTGFHHVGQASFKLPASGGLPASASQSVGITG
VSHWALPVFFISHKSSNKLRIQLIKQAWCLGDL
T*PRCYPVGNHIPLDALQPWFGLESGPVYSILNL
YATGLHCWSGTLVLCIALV*KHVIAVLGIEQTR
RTSNHSTVLPRYTSVLPFPTEVV*G*LFPKAFK
FGF*CDSEFRNQMLDQISSENLRNIVGKVC*ASL
KCHDEHNSRPSWLTPVIPALWEAKVGG*QGQEF
KTSLANMLKPHLY*KYKN*LGMVAHACNPSYLG
G*GRRIA*TREAEVAVSQDSASALQPR*QRKAPS
Q*LKKKKKKKRPGAVAQACNPSTLGG*GGQIT*
GQEF*DQPWQHGETPSLLKIQN*PSMLAHACNP
SYSGG*GTRIA*TWEAEDAVSRDHAIALQPGGQE
*ICVSPKKKRKRKMLNKGYHKPQIHNHLSFQFQ
VEYIHNLNKVLPAPKLS*FIPCALSPSPPSYTH
LQLLACF*HGASGDVLVSDKELWGMSTSA*GFVR
NTCLAVFVTGKELSVLIHLRSPH*S*LCHLSSI
VSPGFLSK*DDRWT*QCLFSLYLASPENHSRIE
IRV*VGSPTSAF

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA
RRLGCRLSAAALGWCAVVRPRLTAASATQM*AI
WFPPQPPE*CLQAYGEQLAWAGPAVPGLHR*LAG
GSRIRLPTCGSSGPPSAACWCWPMPGAGPLSIP
CRMSWSTMSTNGIPRGRPGTTSTTWT*AT*RGY
VVPSWVGPSHPRELCLPRT*SCLQASMHGNNGHS
VPPSKRSETRAPVAPAGPSGLWKPSLTGSASTP
MRTSAWRCRRRTCSHAVAACVGTAVMVAILLKL
GTSGQEKAWFLVASMNPM*GADRTPSLPVSTTST
APGPHARGREIPPSVARSVSLATARPTNRTSTT
DTIPTASPIARRTSWPRSTKTAPWRELSLCIRT
SCSTSQECTNTSPER*WVAMPSASWAGEWRMAHP
TGWLPTPGTLTGVTMASLKYSEDRITVESNQKW
WLEFHAPISTGKRSNLPWACRASPGGEIGVEMH
FIL*VHVRYKFQTGSEGLDWPNIRPVFQGDQVLA
TSQPVVTVQTGHVSHRCQHRASFPL*TSAVGST
CCPS*LWPPP*SIHLQGARQRRRNGKRSS*QDE
SSPISSPSTSKQVAFHICHRNQRRDGVGSPLENA
SLPGPLHLSSLQCHNLSDLVLSMIL**KFYFFV
HSANHVGEPVEQRETCASFTDCLLMTRLKRKPS
GQELFLTH*SLLPQGK*FRRNQLLLFLKNYSFTL
SS*QGMPVPIKVFSNLKSTLMGSQILCHCL*TC
SCCLSLSLQRITSWNCMFLRLLGLHLNFSLPQP
CFQPWHPSPN*FPVILVNLLCKCLVSLPLLKNSK
HSCALFFCCCCD*QSEIPSPRLECSGAFSAHCN
LQPPRFKRFSCFSLPSSWDDRHSPICLGNFCIF
KYIQDFTMLARLVSNSRPQVVCLPQPPKVLGLQA
*ATGPCLYFLSATNPATS*GFSS*NRLGVLVIS
HNQDATPWGTTSPWMPSSLGLGWSQGLYTVF*I
CMPLVCIAGQEL*CFA*PWFRNML*QFLV*SKLE
EPAIIPLSCQGTPQYSPSQLKWYEASSFQKHSS
LASDVTQNLGTRC*IK*ALKI*GTL*ERFVKHL
LSAMMSITAGRRGSRL*SQHFGRPRWEDDKVRSS
RPAWPTC*NLTSTKNTKISWAWWHMPVIPATWE
AEAGESLEPGRRRLQ*AKTVPVHSSLGDSARLR
LNN*KKKKKKKGRAQWLKPVIPALWEAEAGRSPE
VRSFEISLGNTVKPHLY*KYKISQACWHMPVIP
ATREAEVRESLEPGRQRMQ*AEITPLHSSLGDK
SESVSHQKKKEKERCLTKVTISHKFITTYPSSFK
*NIFITSIKFSLLPN*VDSFHAHCLPHLHRIRT
CSSLPASSMEPAGMCW*VTRNCGACRHQLKALL
ETPALQCL*LGKSSVF*YTSDHHIDHNFVTSVQ*
FLLDSCPSEMTGGRNNAFSVFT*PLQRITPE*K
LEYR*AVQPLH

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR
AGWAAGSRLQRWAGVQWCDHGSRQPQPPRCKRS
GSHLSLPSSVFRPMESSLRGLGLQYLVCIDDWQ
VDLGSGFQHVAALGLPLLPAGVGQCPEQALFPSP
VG*AGQLCQQTEYHVAGRAQLLQRGHELLEEAM
WYLPGWAQATPESYVYRGPEAACKLRCTGTMAT
VSHHQRDQRPGLLWLLLGLRGCGSHL*PDLHPHQ
CARQRGGVGGGPAHMLWQHVWGRL*WWLSC*SL
ELLDKKRPGFWWPL*IPCRVQTVLHPSL*APRQ
RLPAPMHGGGRYPQV*QDL*AWLQPDLQTGQALR
IQFLQRLQ*REGHHGRDLQKRPRGGSFLCVFGL
PALQVRSVPTRHRRDDGWPCHPHPGLGSGEWHT
LLAGCQLLEH*LG*QWLL*NTQRTGSLWNRIRSG
GWNSTHRSVLGKDLICRGPVVPVLGARSG*KCI
LFFKFT*DTSFRQGLKDWIGQTSDLSSKETKSW
LHPSLWLQCRQAM*ATAASTERPSPCRLVP*GVP
AAPADCGPLRDPSISREQDRDAGMESGVPNRMK
VPPSVPPVPPSK*LSTFVTEIRGETVLGALWRT
PVSQAPCIYRVCNVTTSLILCSA*FFNRSFIFSC
TLLIMWVSQWNSGRPVLVLQIASL*RGSKGNQV
VRSCF*PTDLYYHKENSLGETSFYCF*KITASP
CQVNKECLCQ*KFSPT*SLL*WDLRSFVTAYRLV
AAVSLCPCRESRPGTACSCDSWDFILTSRCPSH
VFNHGIPPPISSLSSSSTFSVSAW*ACPCLRTQ
NIAVLYFFVVVVTDRVRFRLPGWSAVAPSQLTAT
CSLLDSSDSPASAFRVAGMTGTHQYAWVIFVFL
STYRISPCWPG*FQTPGLRWSACLSLPKCWDYR
REPLGPACIFYQPQIQQQAEDSAHKTGLVSW*SH
ITKMLPRGEPHPPGCPPALVWAGVRACIQYFEF
VCHWFALLVRNSSALHSPGLETCYSSSWYRAN*
KNQQSFHCPAKVHLSTPLPN*SGMRLALSKSIQV
WLLM*LRI*EPDARSNKL*KSEEHCRKGLLSIS
*VP**A*QPAVVAHACNPSTLGGQGGRMTRSGV
QDQPGQHAETSPLLKIQKLAGHGGTCL*SQLLGR
LRQENRLNPGGGGCSEPRQCQCTPASVTAQGSV
SIIKKKKKKKKAGRSGSSL*SQHFGRLRRADHL
RSGVLRSALATR*NPISTKNTKLAKHAGTCL*SQ
LLGRLRYENRLNLGGRGCSEPRSRHCTPAWGTR
VNLCLTKKKKKKKDA*QRLP*ATNS*PLILPVS
SRIYS*PQ*SSPCSQTELIHSMRTVSLTSIVYAP
AAPCLLLAWSQRGCAGE*QGIVGHVDISLRLC*
KHLPCSVCDWERAQCFNTPQITTLIITLSPQFN
SFSWILVQVR*QVDVTMPFQSLPSLSRESLQNRN
*SIGRQSNLCI

Sequence CTSB.qAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG
CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGGTCTTGCTTTGTCTCCCAGGCTGGTGTGCAGTGGTGCGACCACGGCTCACGGCAGCCTCAGC
CACCCAGATGTAAGCGATCTGGTTCCCACCTCAGCCTCCCGAGTAGATACTTCTGAAAATAGAAATGATGACTCTGGGATGCAAACGTTGGCTGTCCTAT
GTATAAGGAGATGGCTTTTCACGCTCCCAGTGACTGAGGAAGTTTCTCCCAGATGGCGCTGCTCTGAGCCTGGTGCAGG
GTGTCTTCAGGCCTATGGAGA
GCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAGATGATTGGCAGGTGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCTCCC
TCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTG
GCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTT
ACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCT
GCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGGGGGATTCGGGGTGGGGACAACTCTGACAA
GGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAATGATGGCGAGGATTAGGTGGAGTGGCGGGC
AGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCCCGTCCGCAAATACCAGGAGCTCTCACTGC
TACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAAATACACGTAACAGAAATGTAATAATCTTA
GCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGGGAT
ATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGACTACAGGCATGTGCCACCACGCCCAGCTA
ATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTGATCTGCCTTCCTCGGCCTCGCAAAATGCT
GGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACATTCACAGTGTTGCTCAGCCACCACCACCAT
CTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCCTGGTAAGCCCCACTCTACTTTCTGTCTCT
ATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTTTTTGTTAAGAAAAAAACACAGCCAGGTAC
GGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTGAGACCAACCTGGCCAACATGGTGAAACCC
TGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGG
AGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCCTCCAGGAAAAAAAAAAAAAACCACACACA
CACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGGGGGTGCTTCTCTTGCTTTAAAAGCTGAGT
TGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAAATCACTTGAACCCGGG
AGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGCCCCAGGAGCGTCCCCTGGTGTTGAGAGAT
CAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGACATGGCAGCCTCACCCGGTGGGGCTCGTTC
CTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACAT
GCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCCTGGGCCCCAGCTTCTCAGTGGAGGGGACT
TTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGCCTGGCAAGGCTGCTGCTTCCTGGGAGGTG
CCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAATGTGGTGGAATGTCTGGCCCCTGGTCTGG
TTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGCGGGGATGGCTCCACCTTAACAACAAGGTG
GTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCGGCTGCAGATCCGAAATGCCTGAGGGCTTA
AAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGGTGGTGTTTTTAAGTACCTTAGGGAAGTTT
AGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGGTATGGACATGAACAGGAGAGCCTCCTGTCTTCTCCCGGAT
CTTTGTGGGCAGGGGTGGGGCTGGCGGTTATTCCCTGCAAGCTGTGCTTATCTAGGGAGCGTCCCTTGGAGGGTTTGGGGTCTGGGAGGTCTGCTCGCAC
CACTTGCTGCAGCTGGGGGTGGGTCCACGAGTGGCCTCGGGCACTTGGGTAGCACACAGTGGTCTGGAGAGCTGGTGGTGCTTCTCTCAGAGGTTTTCAT
TAGAGGCTGTCTTTTCAGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCAT
GTAGGTAAGTGTGTCCCCTTGGCCACTTTCTGGCCAGATGGATTGTTTGAGCAATTAACCATCATGGCTTTATTTGCCTTTATAAACTGGGGGTTGAGAC
AGAGGGGCTGCTGAGAGGTGCTAGCCAGGTGCACAGGCCTCTGGCAGGACCTGCCTGGCGTCCATGCTGCAGGCACGAGGCTTGCCTTGCCCCAGGTCTT
CTCCGTGGGGGCTGTAGGTTGACTCCGCTTTCTCCCGCGTCCCATCAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCG
GCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACGGTAAGGGG
CCTGGGGCCTGGCCACGGCGCACGTGGAGGCTGGGGAGCTGCTGCATCCCTCCTCACGCTGCAGCGAAGAGGTCAGGGCTGAGGAGCCTTGGGGTCCCGG
AACTCTAGGATAGAGGAGGGGGAGTGATGCCCTCTTGCCAGGAGAAGCAGCACACTCTCCACTTTCTGCCTGTTCCACCCTGAACTCAGCCTCAGCCCTT
CCAAACTGGAAGGGACCAAAGCCCTCCTTTTACAAGGGAGTGGCGTGTCCTGGAGTCTTGAGGTATCCTGGCCTCTTCCGGGGCCTCGTGCTCATTGCTC
CGTTCTCCCATTTCTGAGCTTCCAGCCCCAGCCCTGTCCTTAGTTCTTCAGGGGAGCCTTCCTTGAGCTCCCCTGGCAGGGCAAGTCCCCTTAGATGCAG
TCTTCCCCTGGAGGCCACCAGAAATCAGTGACTGGCTGACCGTGGCGTGCCACGAGGCACGGTCACTGCCCTCCCCAACCCCGAGCTCGGTTCATTTTCC
AGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGAC
TTCCTGCTCTACAAGTCAGGTGCGTGCTGATGGCTGATGGCAATAGAGGGTGGGGGTCGGGAGGGGAGCCTGGGTGCCAGGATGGTTCATGTTGACCAAT
AGGGCTGGATTGGGCAGGCAGGTGAGGGGCTGGGGAAGAGGGCTGTGTGGGGCCTTCCATATAGGCTCACTCCTCTGTGGACCAGCCTGGCACCTGACTG
TCTTTGTCCAGGTGTTGTGCCAACACACACAATTCCAGAGGCCTCTCCCAGAGCCTCTGGGCTAGTTCCTTCCCATCCATTAGCGTCCAGCTCATACATG
TTCTCTGTGGGAACGCCCCCCTCCCTGGTGGCTGGCCACGCCTGCCCTCGCCTTCCTGGCTTTGCATTGCTTTACCAGCATGTTTCTGTTATAATGGCAG
GCCGTTCGTGTTTGCACTGGCTGACACATTTTCTTCATCTTTCTCTTTGCAAAGGCCTAGATCGTGCCTCACACCAGTGGATGAACACACAGGGAGGACC
TTGCCCTGGGTAGTGCCATAGGGACTCCAACCAAGGAATCTGGACAGTCCCCATCCCCAAGTCCTGTCTTAGAAATCCCTTGCTCCAGATGAACAGTCTT
TGCTGACTGCATCTATCCCATTAATATTTTGCTAGATTGGAAACTTGTAAAACAACAGTTTGACAAAGGAAGATGATTCTGGCAAGAAAAAGTTTCTGTG
AAGATGGCACTTCTTAGCATAGTCCTCGCCCTTATTATTAAAAGATGCATTTCCACTTTGACAGTGATTTCCCCACCCGAGAGCCTGGTTGGGCAGCCCT
GAGCCCCTTAGCCAGTATCTTTCCCATCAGCTAGCATTAGCTGCCACCAGTCCTCCCTTCTCCTCCCTCACCTCTTCAGGGTGTACCTGGAGCAGCTATT
AAAACTCTTTAAGCCGTGTAACGCTTTTGACAAACCCTTACTTACATGGAATCCCAGTATAAAGAACAGATGAATCAGTCATCTAGCATTAAATGTTTTA
TCAATTAGAATGTATTAGAAATTCTATTTTTAAAGCCAACAAGCACATTTTGTAGGAATCTATTTCAATATTTACTCAACAACTTTGAGTGTGTTGGGCC
TTCAAAAATATATTTAATTCCCCAGTATTGTGGGACCCCCCCTGGAGCCTCCTGGTGGAGCAGCAGGTTCCCTCTGGAAGCTGTTTCTCCTTCCCGGAGC
ATGACCAGCTGAGTGTGGGGGGTGCTGTGGGGCGTGGGACAGTGGCCCGGTCTCGGTCTCAGCCAGTTCTTCCCTTTTCAGGAGTGTACCAACACGTCAC
CGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACTGG
GGTGACAATGGTGAGTGGCTGCCCCCTTCCTGCCAAGAACAGTGAATTGTGAGCCACACCCCGTGGCCATCTCGGCTTTCTCTGTTCTACCCACCGCACA
GCCTTAAACCCTGGACCTACGGCCAGGCTGTGAGCTCCTCCTAAGTGCCAGGCAGTCAGGAGTTCCCTTTTGCTGTGAGGGCAGACTCTGAGCAGCTTCA
GAGCCAACGCCTGCAACAGTTCCCACAGCATCGCGCCAGATCCTGTGATGGGAAGGGTGACCGGGCAGGGGGCTTGCCCGTGGAGGTGTGCCCCACGGCT
CCAGAAGCCTTGTGGTGTTGAGGACTGTGCTAGTGGGCCAACAGCAGGAGTGGCCAGGGATGAGTGACTTAAGGTCTTTTAAGGATGAGTCTGACTATAT
TGGTTGACCCTTGTCACACTTTAAAAGCACCTTACTTTTTATTCCCAGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGT
GGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGGTAGAAATGCA
TTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTG
GCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTA
CCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGA
AAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAAC
GCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCG
TGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAG
TGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACC
CTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTT
GTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCC
ATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCA
AAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCA
ACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTT
TAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG
GCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCT
CACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAAT
TTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTA
GAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAA
GTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCT
CTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGT
TCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGG
AGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCG
TCTCAATAATT
  • CTSB.F2 (forward primer)
  • CTSB.R6 (reverse primer)
  • Single exon skipping

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC
AQAGLQALGCSAGVLLCLPGWCAVVRPRLTAASA
TQM*AIWFPPQPPE*ILLKIEMMTLGCKRWLSY
V*GDGFSRSQ*LRKFLPDGAALSLVQ
GVFRPME
SSLRGLGLQYLVCIDDWQVDLGSGFQHVAALGLP
LLPAGVGQCPEQALFPSPVG*AGQLCQQTEYHV
AGRAQLLQRGHELLEEAMWYLPGWAQATPESYV
YRGPEAACKLRCTGTMATVSHHQRDQRPGLLWLL
LGKALLAGGEALERKWEQHWRVLGDSGWGQL*Q
GKL*KLSESQFPSVQKSQSLWP*MMARIRWSGG
QSIQQIASPRVQVAKQLPFPDMLARPQIPGALTA
TLLQESIPLVSVSCLNFVI*LW*NTRNRNVIIL
AIFFCFLFLSFFFFFFFGDGVLLCHPGWSAVVG
YWLTATSASWVQAILLPQSPQ*LRLQACATTPS*
FLYF**RWGFAMLDSLVSNASPVDLPSSASQNA
GITGVSHRAQQLFSSVQFCSIKYIHSVAQPPPP
SVPRTLFQLARQKLCPH*HQYCSPW*APLYFLSL
*I*LLGTSYKWITTVFIFWVSCVFC*EKNTARY
GSSRL*SQHFGRLRQADHLRSGV*DQPGQHGET
LSLLKRQNLAGPGGRHL*SQLL*RLRQENCLNPG
GVGCSELRLRHCIPAWATKVKLRLQEKKKKPHT
HIQTNTTHFADFRNEPSFQAEVHGGASLALKAE
LGKSLRQESGASGSSRL*SQHFGRPRWANHLNPG
VQDQSGQRSKTPFLPKKIKAEFEPQERPLVLRD
QLPTRSEVPLWPFEGTAAEGPARTWQPHPVGLV
PAGLRGCGSHL*PDLHPHQCARQRGGVGGGPAHM
LWQHVWGRVSQAVLPQRV*C*ETLGPSFSVEGT
LRTSWDCCESEVFRGDSESLAGRAWQGCCFLGG
A*TKAGPAELSEGWHPSKTVSFRKCGGMSGPWSG
LCHRMFLFPAWGTSLGHFLMCS*RGWLHLNNKV
VAQNFLLQLPAASPWFRSSGVHWRLQIRNA*GL
KKMHGLLP*LVKWESLAILISENWWCF*VP*GSL
ASAQLRISVLPGRALVAWSGMDMNRRASCLLPD
LCGQGWGWRLFPASCAYLGSVPWRVWGLGGLLA
PLAAAGGGSTSGLGHLGSTQWSGELVVLLSEVFI
RGCLFSCNGGYPAEAWNFWTRKGLVSGGLYESH
VGKCVPLATFWPDGLFEQLTIMALFAFINWGLR
QRGC*EVLARCTGLWQDLPGVHAAGTRLALPQVF
SVGAVG*LRFLPRPIRVQTVLHPSL*APRQRLP
APMHGGGRYPQV*QDL*AWLQPDLQTGQALR*G
AWGLATAHVEAGELLHPSSRCSEEVRAEEPWGPG
TLG*RRGSDALLPGEAAHSPLSACSTLNSASAL
PNWKGPKPSFYKGVACPGVLRYPGLFRGLVLIA
PFSHF*ASSPSPVLSSSGEPSLSSPGRASPLRCS
LPLEATRNQ*LADRGVPRGTVTALPNPELGSFS
RIQFLQRLQ*REGHHGRDLQKRPRGGSFLCVFG
LPALQVRCVLMADGNRGWGSGGEPGCQDGSC*PI
GLDWAGR*GAGEEGCVGPSI*AHSSVDQPGT*L
SLSRCCANTHNSRGLSQSLWASSFPSISVQLIH
VLCGNAPLPGGWPRLPSPSWLCIALPACFCYNGR
PFVFALADTFSSSFSLQRPRSCLTPVDEHTGRT
LPWVVP*GLQPRNLDSPHPQVLS*KSLAPDEQS
LLTASIPLIFC*IGNL*NNSLTKEDDSGKKKFL*
RWHFLA*SSPLLLKDAFPL*Q*FPHPRAWLGSP
EPLSQYLSHQLALAATSPPFSSLTSSGCTWSSY
*NSLSRVTLLTNPYLHGIPV*RTDESVI*H*MFY
QLECIRNSIFKANKHIL*ESISIFTQQL*VCWA
FKNIFNSPVLWDPPWSLLVEQQVPSGSCFSFPE
HDQLSVGGAVGRGTVARSRSQPVLPFSGVYQHVT
GEMMGGHAIRILGWGVENGTPYWLVANSWNTDW
GDNGEWLPPSCQEQ*IVSHTPWPSRLSLFYPPH
SLKPWTYGQAVSSS*VPGSQEFPFAVRADSEQLQ
SQRLQQFPQHRARSCDGKGDRAGGLPVEVCPTA
PEALWC*GLC*WANSRSGQG*VT*GLLRMSLTI
LVDPCHTLKAPYFLFPGFFKILRGQDHCGIESEV
VAGIPRTDQYWEKI*SAVGLSCQSWGRDRGRNA
FYSLSSRKIQVSDRV*RTGLAKHQTCLPRRPSP
GYIPACGYSADRPCEPPLPAQSVLPPVD*CRREY
LLPQLTVAPSVIHPSPGSKTETQEWKAEFLTG*
KFPHQFPQYLQASSFPHLSQKSEERRCWEPFGE
RQSPRPPASIEFAMSQPL*SCAQHDSLIEVLFFR
ALC*SCG*ASGTAGDLC*FYRLPPYDAAQKETK
WSGVVSDPLISTTTRKIV*EKPAFTVFEKLQLH
PVKLTRNACANKSFLQLEVYSDGISDPLSLPIDL
*LLSLFVPAENHVLELHVLATLGTSS*LLAAPA
MFSTMASLPQLVPCHPRQPSL*VPGKLALA*EL
KT*LCSIFLLLL*LTE*DSVSQAGVQWRLLSSLQ
PAAS*IQAILLLQPSE*LG*QALTNMPG*FLYF
*VHTGFHHVGQASFKLPASGGLPASASQSVGIT
GVSHWALPVFFISHKSSNKLRIQLIKQAWCLGDL
T*PRCYPVGNHIPLDALQPWFGLESGPVYSILN
LYATGLHCWSGTLVLCIALV*KHVIAVLGIEQT
RRTSNHSTVLPRYTSVLPFPTEVV*G*LFPKAFK
FGF*CDSEFRNQMLDQISSENLRNIVGKVC*AS
LKCHDEHNSRPSWLTPVIPALWEAKVGG*QGQE
FKTSLANMLKPHLY*KYKN*LGMVAHACNPSYLG
G*GRRIA*TREAEVAVSQDSASALQPR*QRKAP
SQ*

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA
RRLGCRLSAAALGSCFVSQAGVQWCDHGSRQPQ
PPRCKRSGSHLSLPSRYF*K*K**LWDANVGCPM
YKEMAFHAPSD*GSFSQMALL*AWCR
VSSGLWR
AACVGWACSTWFA*MIGRWI*DPASNMWQLWAS
LCCLLVLANARSRPSFHPLSDELVNYVNKRNTTW
QAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMF
TEDLKLPASFDAREQWPQCPTIKEIRDQGSCGS
CWVRPCWLAGKRWRESGSNTGESWGIRGGDNSDK
ASYRNFLSPSFHQYKNHNPSGHE*WRGLGGVAG
RASSRLQVHVYRWRSSSLSLTCWPVRKYQELSL
LLCFKKASL*CQ*AVLILSFNCGKIHVTEM**S*
QSSFVFFFFLFFFFFFLEMESCSVTQAGVQWWD
IGSLQPLPPGFKQFSCLSLPSS*DYRHVPPRPA
NFCIFSRDGVLPCWTAWSQTPHLLICLPRPRKML
GLQA*ATVPSNYFQVYSSVALSTFTVLLSHHHH
LSPELFFSSQDRNSVPINTNIVAPGKPHSTFCL
YEFDS*GPHTSGSQQYLFSG*AVFFVKKKTQPGT
VAHACNLNTLGG*GRRIT*GQEFETNLANMVKP
CLY*KDKT*LGLVAGTYNPSYYRG*GRRIA*TQ
EA*VAVS*DCATAFQPGQQK*NSASRKKKKNHTH
TYKLILHILQISEMNPVSRQRFTGVLLLL*KLS
WANR*GRRVGQVAHHASNPSTLGGQGGQIT*TR
EFKTSLANVARPHFYQRK*KLSLSPRSVPWC*EI
SCLQGLRCPCGPLRGLLQRAQPGHGSLTRWGSF
LQAFGAVEAISDRICIHTNAHVSVEVSAEDLLT
CCGSMCGDG*VRLCFHSGFSAERPWAPASQWRGL
*GLPGTAASQKCFGETPRVWQAGPGKAAASWEV
PEPRLARQSCLRGGIPAKQSVSENVVECLAPGL
VCVIACSFFLLGEPVWGISLCVVSGDGSTLTTRW
WHRTFCSSCLQPRLGSAVAVFTGGCRSEMPEGL
KKCMGFSPN*LNGNHWRFSFQRTGGVFKYLREV
*RLLS*ESVFYPEGHS*PGLVWT*TGEPPVFSRI
FVGRGGAGGYSLQAVLI*GASLGGFGVWEVCSH
HLLQLGVGPRVASGTWVAHSGLESWWCFSQRFS
LEAVFSAVMVAILLKLGTSGQEKAWFLVASMNPM
*VSVSPWPLSGQMDCLSN*PSWLYLPL*TGG*D
RGAAERC*PGAQASGRTCLASMLQARGLPCPRS
SPWGL*VDSAFSRVPSGCRPYSIPPCEHHVNGSR
PPCTGEGDTPKCSKICEPGYSPTYKQDKHYGKG
PGAWPRRTWRLGSCCIPPHAAAKRSGLRSLGVP
EL*DRGGGVMPSCQEKQHTLHFLPVPP*TQPQPF
QTGRDQSPPFTREWRVLES*GILASSGASCSLL
RSPISELPAPALSLVLQGSLP*APLAGQVPLDA
VFPWRPPEISDWLTVACHEARSLPSPTPSSVHFP
GYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSD
FLLYKSGAC*WLMAIEGGGREGSLGARMVHVDQ
*GWIGQAGEGLGKRAVWGLPYRLTPLWTSLAPDC
LCPGVVPTHTIPEASPRASGLVPSHPLASSSYM
FSVGTPPSLVAGHACPRLPGFALLYQHVSVIMA
GRSCLHWLTHFLHLSLCKGLDRASHQWMNTQGGP
CPG*CHRDSNQGIWTVPIPKSCLRNPLLQMNSL
C*LHLSH*YFARLETCKTTV*QRKMILARKSFC
EDGTS*HSPRPYY*KMHFHFDSDFPTREPGWAAL
SPLASIFPIS*H*LPPVLPSPPSPLQGVPGAAI
KTL*AV*RF*QTLTYMESQYKEQMNQSSSIKCF
IN*NVLEILFLKPTSTFCRNLFQYLLNNFECVGP
SKIYLIPQYCGTPPGASWWSSRFPLEAVSPSRS
MTS*VWGVLWGVGQWPGLGLSQFFPFQECTNTS
PER*WVAMPSASWAGEWRMAHPTGWLPTPGTLTG
VTMVSGCPLPAKNSEL*ATPRGHLGFLCSTHRT
ALNPGPTARL*APPKCQAVRSSLLL*GQTLSSF
RANACNSSHSIAPDPVMGRVTGQGACPWRCAPRL
QKPCGVEDCASGPTAGVARDE*LKVF*G*V*LY
WLTLVTL*KHLTFYSQASLKYSEDRITVESNQK
WWLEFHAPISTGKRSNLPWACRASPGGEIGVEMH
FIL*VHVRYKFQTGSEGLDWPNIRPVFQGDQVL
ATSQPVVTVQTGHVSHRCQHRASFPL*TSAVGS
TCCPS*LWPPP*SIHLQGARQRRRNGKRSS*QDE
SSPISSPSTSKQVAFHICHRNQRRDGVGSPLEN
ASLPGPLHLSSLQCHNLSDLVLSMIL**KFYFF
VHSANHVGEPVEQRETCASFTDCLLMTRLKRKPS
GQELFLTH*SLLPQGK*FRRNQLLLFLKNYSFT
LSS*QGMPVPIKVFSNLKSTLMGSQILCHCL*T
CSCCLSLSLQRITSWNCMFLRLLGLHLNFSLPQP
CFQPWHPSPN*FPVILVNLLCKCLVSLPLLKNS
KHSCALFFCCCCD*QSEIPSPRLECSGAFSAHC
NLQPPRFKRFSCFSLPSSWDDRHSPICLGNFCIF
KYIQDFTMLARLVSNSRPQVVCLPQPPKVLGLQ
A*ATGPCLYFLSATNPATS*GFSS*NRLGVLVI
SHNQDATPWGTTSPWMPSSLGLGWSQGLYTVF*I
CMPLVCIAGQEL*CFA*PWFRNML*QFLV*SKL
EEPAIIPLSCQGTPQYSPSQLKWYEASSFQKHS
SLASDVTQNLGTRC*IK*ALKI*GTL*ERFVKHL
LSAMMSITAGRRGSRL*SQHFGRPRWEDDKVRS
SRPAWPTC*NLTSTKNTKISWAWWHMPVIPATW
EAEAGESLEPGRRRLQ*AKTVPVHSSLGDSARLR
LNN

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR
AGWAAGSRLQRWGLALSPRLVCSGATTAHGSLS
HPDVSDLVPTSASRVDTSENRNDDSGMQTLAVL
CIRRWLFTLPVTEEVSPRWRCSEPGAG
CLQAYGE
QLAWAGPAVPGLHR*LAGGSRIRLPTCGSSGPP
SAACWCWPMPGAGPLSIPCRMSWSTMSTNGIPR
GRPGTTSTTWT*AT*RGYVVPSWVGPSHPRELCL
PRT*SCLQASMHGNNGHSVPPSKRSETRAPVAP
AG*GPAGWRGSAGEKVGATLESLGGFGVGTTLT
RQVIETF*VPVSISTKITIPLAMNDGED*VEWRA
EHPADCKSTCTGGEAAPFP*HAGPSANTRSSHC
YSASRKHPFSVSELS*FCHLIVVKYT*QKCNNL
SNLLLFSFSFFFFFFFFWRWSLALSPRLECSGGI
LAHCNLCLLGSSNSPASVSPVAKTTGMCHHAQL
IFVFLVEMGFCHVGQPGLKRLTC*SAFLGLAKC
WDYRREPPCPATIFKCTVL*H*VHSQCCSATTTI
CPPNSFSARKTETLSPLTPIL*PLVSPTLLSVS
MNLTPRDLIQVDHNSIYFLGELCFLLRKKHSQV
R*LTPVISTLWEAEAGGSPEVRSLRPTWPTW*NP
VSTKKTKLSWAWWQAPIIPATIEAEAGELLEPR
RRRLQ*AEIAPLHSSLGNKSETPPPGKKKKTTH
THTN*YYTFCRFQK*TQFPGRGSRGCFSCFKS*V
GQIVEAGEWGKWLITPLIPALWEAKVGKSLEPG
SSRPVWPT*QDPISTKENKS*V*APGASPGVER
SVAYKV*GAPVAL*GDCCRGPSPDMAASPGGARS
CRPSGLWKPSLTGSASTPMRTSAWRCRRRTCSH
AVAACVGTGESGCASTAGLVLRDPGPQLLSGGD
FEDFLGLLRVRSVSGRLRESGRQGLARLLLPGRC
LNQGWPGRAV*GVASQQNSQFQKMWWNVWPLVW
FVSSHVPFSCLGNQFGAFPYV*LAGMAPP*QQG
GGTELSAPAACSLALVPQ*RCSLAAADPKCLRA*
KNAWASPLTS*MGITGDSHFRELVVFLSTLGKF
SVCSVENQCSTRKGTRSLVWYGHEQESLLSSPG
SLWAGVGLAVIPCKLCLSRERPLEGLGSGRSART
TCCSWGWVHEWPRALG*HTVVWRAGGASLRGFH
*RLSFQL*WWLSC*SLELLDKKRPGFWWPL*IP
CR*VCPLGHFLARWIV*AINHHGFICLYKLGVET
EGLLRGASQVHRPLAGPAWRPCCRHEACLAPGL
LRGGCRLTPLSPASHQGADRTPSLPVSTTSTAP
GPHARGREIPPSVARSVSLATARPTNRTSTTVRG
LGPGHGARGGWGAAASLLTLQRRGQG*GALGSR
NSRIEEGE*CPLARRSSTLSTFCLFHPELSLSP
SKLEGTKALLLQGSGVSWSLEVSWPLPGPRAHCS
VLPFLSFQPQPCP*FFRGAFLELPWQGKSP*MQ
SSPGGHQKSVTG*PWRATRHGHCPPQPRARFIF
QDTIPTASPIARRTSWPRSTKTAPWRELSLCIRT
SCSTSQVRADG*WQ*RVGVGRGAWVPGWFMLTN
RAGLGRQVRGWGRGLCGAFHIGSLLCGPAWHLT
VFVQVLCQHTQFQRPLPEPLG*FLPIH*RPAHTC
SLWERPPPWWLATPALAFLALHCFTSMFLL*WQ
AVRVCTG*HIFFIFLFAKA*IVPHTSG*THRED
LALGSAIGTPTKESGQSPSPSPVLEIPCSR*TVF
ADCIYPINILLDWKLVKQQFDKGR*FWQEKVSV
KMALLSIVLALIIKRCISTLTVISPPESLVGQP
*AP*PVSFPSASISCHQSSLLLPHLFRVYLEQLL
KLFKPCNAFDKPLLTWNPSIKNR*ISHLALNVL
SIRMY*KFYF*SQQAHFVGIYFNIYSTTLSVLG
LQKYI*FPSIVGPPLEPPGGAAGSLWKLFLLPGA
*PAECGGCCGAWDSGPVSVSASSSLFRSVPTRH
RRDDGWPCHPHPGLGSGEWHTLLAGCQLLEH*L
G*QW*VAAPFLPRTVNCEPHPVAISAFSVLPTAQ
P*TLDLRPGCELLLSARQSGVPFCCEGRL*AAS
EPTPATVPTASRQIL*WEG*PGRGLARGGVPHG
SRSLVVLRTVLVGQQQEWPGMSDLRSFKDESDYI
G*PLSHFKSTLLFIPRLL*NTQRTGSLWNRIRS
GGWNSTHRSVLGKDLICRGPVVPVLGARSG*KC
ILFFKFT*DTSFRQGLKDWIGQTSDLSSKETKSW
LHPSLWLQCRQAM*ATAASTERPSPCRLVP*GV
PAAPADCGPLRDPSISREQDRDAGMESGVPNRM
KVPPSVPPVPPSK*LSTFVTEIRGETVLGALWRT
PVSQAPCIYRVCNVTTSLILCSA*FFNRSFIFS
CTLLIMWVSQWNSGRPVLVLQIASL*RGSKGNQ
VVRSCF*PTDLYYHKENSLGETSFYCF*KITASP
CQVNKECLCQ*KFSPT*SLL*WDLRSFVTAYRL
VAAVSLCPCRESRPGTACSCDSWDFILTSRCPS
HVFNHGIPPPISSLSSSSTFSVSAW*ACPCLRTQ
NIAVLYFFVVVVTDRVRFRLPGWSAVAPSQLTA
TCSLLDSSDSPASAFRVAGMTGTHQYAWVIFVF
LSTYRISPCWPG*FQTPGLRWSACLSLPKCWDYR
REPLGPACIFYQPQIQQQAEDSAHKTGLVSW*S
HITKMLPRGEPHPPGCPPALVWAGVRACIQYFE
FVCHWFALLVRNSSALHSPGLETCYSSSWYRAN*
KNQQSFHCPAKVHLSTPLPN*SGMRLALSKSIQ
VWLLM*LRI*EPDARSNKL*KSEEHCRKGLLSI
S*VP**A*QPAVVAHACNPSTLGGQGGRMTRSGV
QDQPGQHAETSPLLKIQKLAGHGGTCL*SQLLG
RLRQENRLNPGGGGCSEPRQCQCTPASVTAQGS
VSII

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