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Sequences

Sequence CTSB.lAug05

CACCCCCAGTGTTTTCTATTGTGCTTCTCCAAGGTGTCTCAGCCGCAGATGTGACAGACAAGGTCTGGGTCCCCACTATGTGGGCAGACTCAATCTTGAG TAATTTAAGGGAAATCTAAGAAAAGCCAGATGAGGCCAAGTATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGATGCCAAGGCAAGCGGATCACCTG AGGTCAGGAGTTCGAGACCAGTCTGACCAACATGATGAAACCCCGTCTCTACTAAAAATAAAAAACTTTGCCAGGCGTGGTGGCGGGCGCCTGTAATCCC AGCTACTCGGGAGGCTGAGGCTAGAGAATCGCATGAACCCGGGAGGCAGAGGTTGCGGTGAGCTGAGATGATGCCACTTCCCACGTGAGGATGGGGTAGT TAAGTTGCACGACACTGCCTTCTGCACAATGGGGATGATATGAGAAATGATGAGAGGATCCTGGGCAGAGGTTGAGGCAGGAGAGCAGGGCAAGAAACAC TATCTGGACTGAATAAACTTATTTAAAAATGAGTTTATAATTTGTGGATAAATTCATCTTAAAAAATCCATTACATAAGCCTAGAAAGAAGCGTACAATA TCGCAAGTCAGGCCAGGTGTGGTGGATCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCATTTGAAGCCAGGAGTTTAAGACCAGCC TGGCCAACATGGTGAAACCCTGTCTTTACTAAAAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAC GAGAATCGATTGAACTCAGGAGGCAGAGGTTGCAGTGGGCAGAGATCTCGCCACTGCACTCCAGCCTGGATGACACAGTGAAATTCTGTCCACTCCCACC CTCCCGTCCCACCCAAAAAAAAAATCAAAAGGTGCATTGAGCAACAGAATTTCCTTTTGTTTTATGAAATTACCAAGGACACCTGTCTGCAGCCTTCTTC GGCATGTCTTGTGCTCATTTCCTTGTTAGGGGAGTGTGGCCCAGCCAAAGTGGCCTTTGCAGGGATTGGGGAGGGAGTTGTGGGATCAGAGCTTGTAATG AAGACCTTCCTTTATCCAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGA GATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGG GGGATTCGGGGTGGGGACAACTCTGACAAGGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAAT GATGGCGAGGATTAGGTGGAGTGGCGGGCAGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCC CGTCCGCAAATACCAGGAGCTCTCACTGCTACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAA ATACACGTAACAGAAATGTAATAATCTTAGCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTG TCACCCAGGCTGGAGTGCAGTGGTGGGATATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGA CTACAGGCATGTGCCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTG ATCTGCCTTCCTCGGCCTCGCAAAATGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACAT TCACAGTGTTGCTCAGCCACCACCACCATCTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCC TGGTAAGCCCCACTCTACTTTCTGTCTCTATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTT TTTGTTAAGAAAAAAACACAGCCAGGTACGGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTG AGACCAACCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGG CTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCC TCCAGGAAAAAAAAAAAAAACCACACACACACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGG GGGTGCTTCTCTTGCTTTAAAAGCTGAGTTGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGG CCAAGGTGGGCAAATCACTTGAACCCGGGAGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGC CCCAGGAGCGTCCCCTGGTGTTGAGAGATCAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGAC ATGGCAGCCTCACCCGGTGGGGCTCGTTCCTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGT GGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCC TGGGCCCCAGCTTCTCAGTGGAGGGGACTTTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGC CTGGCAAGGCTGCTGCTTCCTGGGAGGTGCCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAA TGTGGTGGAATGTCTGGCCCCTGGTCTGGTTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGC GGGGATGGCTCCACCTTAACAACAAGGTGGTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCG GCTGCAGATCCGAAATGCCTGAGGGCTTAAAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGG TGGTGTTTTTAAGTACCTTAGGGAAGTTTAGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGCTGTAATGGTGG CTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGGTGCAGACCGTACTCCATCCCTCCC TGTGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACA AACAGGACAAGCACTACGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTT CTCTGTGTATTCGGACTTCCTGCTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGA GTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTG GAATCGAATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGGTAGAAATGCATTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGA CAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTGGCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCA TGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTACCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCC ATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGAAAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAG TAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAACGCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCA ATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAG CGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAGTGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTAC CACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACCCTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGT TTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTTGTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACG TCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCCATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCC CTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCAAAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGA CTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCAACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTC AGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTTTAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAG TTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGC CACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCTCACATAACCAAGATGCTACCCCGTGGGGAACCACATCC CCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAATTTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCT AGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTAC ACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAAGTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGA TGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCTCTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTG GCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTC TACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGG AGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCGTCTCAATAATTAAAAAAAAAAAAAAAAAAAAAAAGGCC GGGCGCAGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGAGGTCAGGAGTTTTGAGATCAGCCTTGGCAACACGGT GAAACCCCATCTCTACTAAAAATACAAAATTAGCCAAGCATGCTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGTACGAGAATCGCTTGAA CCTGGGAGGCAGAGGATGCAGTGAGCCGAGATCACGCCATTGCACTCCAGCCTGGGGGACAAGAGTGAATCTGTGTCTCACCAAAAAAAAAAAGAAAAAG AAAGATGCTTAACAAAGGTTACCATAAGCCACAAATTCATAACCACTTATCCTTCCAGTTTCAAGTAGAATATATTCATAACCTCAATAAAGTTCTCCCT GCTCCCAAACTGAGTTGATTCATTCCATGCGCACTGTCTCCCTCACCTCCATCGTATACGCACCTGCAGCTCCTTGCCTGCTTCTAGCATGGAGCCAGCG GGGATGTGCTGGTGAGTGACAAGGAATTGTGGGGCATGTCGACATCAGCTTAAGGCTTTGTTAGAAACACCTGCCTTGCAGTGTTTGTGACTGGGAAAGA GCTCAGTGTTTTAATACACCTCAGATCACCACATTGATCATAACTTTGTCACCTCAGTTCAATAGTTTCTCCTGGATTCTTGTCCAAGTGAGATGACAGG TGGACGTAACAATGCCTTTTCAGTCTTTACCTAGCCTCTCCAGAGAATCACTCCAGAATAGAAATTAGAGTATAGGTAGGCAGTCCAACCTCTGCATTT

CTSB.F10 (forward primer)
CTSB.R7 (reverse primer)
Intron retention

Frame 0

HPQCFLLCFSKVSQPQM*QTRSGSPLCGQTQS* VI*GKSKKSQMRPSMVAHACNPSTLGCQGKRIT* GQEFETSLTNMMKPRLY*K*KTLPGVVAGACNP SYSGG*G*RIA*TREAEVAVS*DDATSHVRMG* LSCTTLPSAQWG*YEK**EDPGQRLRQESRARNT IWTE*TYLKMSL*FVDKFILKNPLHKPRKKRTI SQVRPGVVDHACNPSTLGGQGRQII*SQEFKTS LANMVKPCLY*KYKN*PGVVVHACNPSYSGG*GT RID*TQEAEVAVGRDLATALQPG*HSEILSTPT LPSHPKKKSKGALSNRISFCFMKLPRTPVCSLL RHVLCSFPC*GSVAQPKWPLQGLGRELWDQSL** RPSFIQSYVYRGPEAACKLRCTGTMATVSHHQR DQRPGLLWLLLGKALLAGGEALERKWEQHWRVL GDSGWGQL*QGKL*KLSESQFPSVQKSQSLWP*M MARIRWSGGQSIQQIASPRVQVAKQLPFPDMLA RPQIPGALTATLLQESIPLVSVSCLNFVI*LW* NTRNRNVIILAIFFCFLFLSFFFFFFFGDGVLLC HPGWSAVVGYWLTATSASWVQAILLPQSPQ*LR LQACATTPS*FLYF**RWGFAMLDSLVSNASPV DLPSSASQNAGITGVSHRAQQLFSSVQFCSIKYI HSVAQPPPPSVPRTLFQLARQKLCPH*HQYCSP W*APLYFLSL*I*LLGTSYKWITTVFIFWVSCV FC*EKNTARYGSSRL*SQHFGRLRQADHLRSGV* DQPGQHGETLSLLKRQNLAGPGGRHL*SQLL*R LRQENCLNPGGVGCSELRLRHCIPAWATKVKLR LQEKKKKPHTHIQTNTTHFADFRNEPSFQAEVHG GASLALKAELGKSLRQESGASGSSRL*SQHFGR PRWANHLNPGVQDQSGQRSKTPFLPKKIKAEFE PQERPLVLRDQLPTRSEVPLWPFEGTAAEGPART WQPHPVGLVPAGLRGCGSHL*PDLHPHQCARQR GGVGGGPAHMLWQHVWGRVSQAVLPQRV*C*ET LGPSFSVEGTLRTSWDCCESEVFRGDSESLAGRA WQGCCFLGGA*TKAGPAELSEGWHPSKTVSFRK CGGMSGPWSGLCHRMFLFPAWGTSLGHFLMCS* RGWLHLNNKVVAQNFLLQLPAASPWFRSSGVHWR LQIRNA*GLKKMHGLLP*LVKWESLAILISENW WCF*VP*GSLASAQLRISVLPGRALVAWSAVMV AILLKLGTSGQEKAWFLVASMNPM*GADRTPSLP VSTTSTAPGPHARGREIPPSVARSVSLATARPT NRTSTTDTIPTASPIARRTSWPRSTKTAPWREL SLCIRTSCSTSQECTNTSPER*WVAMPSASWAGE WRMAHPTGWLPTPGTLTGVTMASLKYSEDRITV ESNQKWWLEFHAPISTGVEMHFIL*VHVRYKFQ TGSEGLDWPNIRPVFQGDQVLATSQPVVTVQTGH VSHRCQHRASFPL*TSAVGSTCCPS*LWPPP*S IHLQGARQRRRNGKRSS*QDESSPISSPSTSKQ VAFHICHRNQRRDGVGSPLENASLPGPLHLSSLQ CHNLSDLVLSMIL**KFYFFVHSANHVGEPVEQ RETCASFTDCLLMTRLKRKPSGQELFLTH*SLL PQGK*FRRNQLLLFLKNYSFTLSS*QGMPVPIKV FSNLKSTLMGSQILCHCL*TCSCCLSLSLQRIT SWNCMFLRLLGLHLNFSLPQPCFQPWHPSPN*F PVILVNLLCKCLVSLPLLKNSKHSCALFFCCCCD *QSEIPSPRLECSGAFSAHCNLQPPRFKRFSCF SLPSSWDDRHSPICLGNFCIFKYIQDFTMLARL VSNSRPQVVCLPQPPKVLGLQA*ATGPCLYFLSA TNPATS*GFSS*NRLGVLVISHNQDATPWGTTS PWMPSSLGLGWSQGLYTVF*ICMPLVCIAGQEL *CFA*PWFRNML*QFLV*SKLEEPAIIPLSCQGT PQYSPSQLKWYEASSFQKHSSLASDVTQNLGTR C*IK*ALKI*GTL*ERFVKHLLSAMMSITAGRR GSRL*SQHFGRPRWEDDKVRSSRPAWPTC*NLTS TKNTKISWAWWHMPVIPATWEAEAGESLEPGRR RLQ*AKTVPVHSSLGDSARLRLNN*KKKKKKKG RAQWLKPVIPALWEAEAGRSPEVRSFEISLGNTV KPHLY*KYKISQACWHMPVIPATREAEVRESLE PGRQRMQ*AEITPLHSSLGDKSESVSHQKKKEK ERCLTKVTISHKFITTYPSSFK*NIFITSIKFSL LPN*VDSFHAHCLPHLHRIRTCSSLPASSMEPA GMCW*VTRNCGACRHQLKALLETPALQCL*LGK SSVF*YTSDHHIDHNFVTSVQ*FLLDSCPSEMTG GRNNAFSVFT*PLQRITPE*KLEYR*AVQPLH

Frame 1

TPSVFYCASPRCLSRRCDRQGLGPHYVGRLNLE *FKGNLRKAR*GQVWWLTPVIPALWDAKASGSP EVRSSRPV*PT**NPVSTKNKKLCQAWWRAPVIP ATREAEARESHEPGRQRLR*AEMMPLPT*GWGS *VARHCLLHNGDDMRNDERILGRG*GRRAGQET LSGLNKLI*K*VYNLWINSS*KIHYISLERSVQY RKSGQVWWIMPVIPALWEAKAGRSFEARSLRPA WPTW*NPVFTKNTKISRVWWCMPVIPATQEAEA RESIELRRQRLQWAEISPLHSSLDDTVKFCPLPP SRPTQKKNQKVH*ATEFPFVL*NYQGHLSAAFF GMSCAHFLVRGVWPSQSGLCRDWGGSCGIRACN EDLPLSRVMFTEDLKLPASFDAREQWPQCPTIKE IRDQGSCGSCWVRPCWLAGKRWRESGSNTGESW GIRGGDNSDKASYRNFLSPSFHQYKNHNPSGHE *WRGLGGVAGRASSRLQVHVYRWRSSSLSLTCWP VRKYQELSLLLCFKKASL*CQ*AVLILSFNCGK IHVTEM**S*QSSFVFFFFLFFFFFFLEMESCS VTQAGVQWWDIGSLQPLPPGFKQFSCLSLPSS*D YRHVPPRPANFCIFSRDGVLPCWTAWSQTPHLL ICLPRPRKMLGLQA*ATVPSNYFQVYSSVALST FTVLLSHHHHLSPELFFSSQDRNSVPINTNIVAP GKPHSTFCLYEFDS*GPHTSGSQQYLFSG*AVF FVKKKTQPGTVAHACNLNTLGG*GRRIT*GQEF ETNLANMVKPCLY*KDKT*LGLVAGTYNPSYYRG *GRRIA*TQEA*VAVS*DCATAFQPGQQK*NSA SRKKKKNHTHTYKLILHILQISEMNPVSRQRFT GVLLLL*KLSWANR*GRRVGQVAHHASNPSTLGG QGGQIT*TREFKTSLANVARPHFYQRK*KLSLS PRSVPWC*EISCLQGLRCPCGPLRGLLQRAQPG HGSLTRWGSFLQAFGAVEAISDRICIHTNAHVSV EVSAEDLLTCCGSMCGDG*VRLCFHSGFSAERP WAPASQWRGL*GLPGTAASQKCFGETPRVWQAG PGKAAASWEVPEPRLARQSCLRGGIPAKQSVSEN VVECLAPGLVCVIACSFFLLGEPVWGISLCVVS GDGSTLTTRWWHRTFCSSCLQPRLGSAVAVFTG GCRSEMPEGLKKCMGFSPN*LNGNHWRFSFQRTG GVFKYLREV*RLLS*ESVFYPEGHS*PGLL*WW LSC*SLELLDKKRPGFWWPL*IPCRVQTVLHPS L*APRQRLPAPMHGGGRYPQV*QDL*AWLQPDLQ TGQALRIQFLQRLQ*REGHHGRDLQKRPRGGSF LCVFGLPALQVRSVPTRHRRDDGWPCHPHPGLG SGEWHTLLAGCQLLEH*LG*QWLL*NTQRTGSLW NRIRSGGWNSTHRSVLG*KCILFFKFT*DTSFR QGLKDWIGQTSDLSSKETKSWLHPSLWLQCRQA M*ATAASTERPSPCRLVP*GVPAAPADCGPLRDP SISREQDRDAGMESGVPNRMKVPPSVPPVPPSK *LSTFVTEIRGETVLGALWRTPVSQAPCIYRVC NVTTSLILCSA*FFNRSFIFSCTLLIMWVSQWNS GRPVLVLQIASL*RGSKGNQVVRSCF*PTDLYY HKENSLGETSFYCF*KITASPCQVNKECLCQ*K FSPT*SLL*WDLRSFVTAYRLVAAVSLCPCRESR PGTACSCDSWDFILTSRCPSHVFNHGIPPPISS LSSSSTFSVSAW*ACPCLRTQNIAVLYFFVVVV TDRVRFRLPGWSAVAPSQLTATCSLLDSSDSPAS AFRVAGMTGTHQYAWVIFVFLSTYRISPCWPG* FQTPGLRWSACLSLPKCWDYRREPLGPACIFYQ PQIQQQAEDSAHKTGLVSW*SHITKMLPRGEPHP PGCPPALVWAGVRACIQYFEFVCHWFALLVRNS SALHSPGLETCYSSSWYRAN*KNQQSFHCPAKV HLSTPLPN*SGMRLALSKSIQVWLLM*LRI*EPD ARSNKL*KSEEHCRKGLLSIS*VP**A*QPAVV AHACNPSTLGGQGGRMTRSGVQDQPGQHAETSP LLKIQKLAGHGGTCL*SQLLGRLRQENRLNPGGG GCSEPRQCQCTPASVTAQGSVSIIKKKKKKKKA GRSGSSL*SQHFGRLRRADHLRSGVLRSALATR *NPISTKNTKLAKHAGTCL*SQLLGRLRYENRLN LGGRGCSEPRSRHCTPAWGTRVNLCLTKKKKKK KDA*QRLP*ATNS*PLILPVSSRIYS*PQ*SSP CSQTELIHSMRTVSLTSIVYAPAAPCLLLAWSQR GCAGE*QGIVGHVDISLRLC*KHLPCSVCDWER AQCFNTPQITTLIITLSPQFNSFSWILVQVR*Q VDVTMPFQSLPSLSRESLQNRN*SIGRQSNLCI

Frame 2

PPVFSIVLLQGVSAADVTDKVWVPTMWADSILS NLREI*EKPDEAKYGGSRL*SQHFGMPRQADHL RSGVRDQSDQHDETPSLLKIKNFARRGGGRL*S QLLGRLRLENRMNPGGRGCGELR*CHFPREDGVV KLHDTAFCTMGMI*EMMRGSWAEVEAGEQGKKH YLD*INLFKNEFIICG*IHLKKSIT*A*KEAYN IASQARCGGSCL*SQHFGRPRQADHLKPGV*DQP GQHGETLSLLKIQKLAGCGGACL*SQLLRRLRH ENRLNSGGRGCSGQRSRHCTPAWMTQ*NSVHSH PPVPPKKKIKRCIEQQNFLLFYEITKDTCLQPSS ACLVLISLLGECGPAKVAFAGIGEGVVGSELVM KTFLYPELCLPRT*SCLQASMHGNNGHSVPPSK RSETRAPVAPAG*GPAGWRGSAGEKVGATLESLG GFGVGTTLTRQVIETF*VPVSISTKITIPLAMN DGED*VEWRAEHPADCKSTCTGGEAAPFP*HAG PSANTRSSHCYSASRKHPFSVSELS*FCHLIVVK YT*QKCNNLSNLLLFSFSFFFFFFFFWRWSLAL SPRLECSGGILAHCNLCLLGSSNSPASVSPVAK TTGMCHHAQLIFVFLVEMGFCHVGQPGLKRLTC* SAFLGLAKCWDYRREPPCPATIFKCTVL*H*VH SQCCSATTTICPPNSFSARKTETLSPLTPIL*P LVSPTLLSVSMNLTPRDLIQVDHNSIYFLGELCF LLRKKHSQVR*LTPVISTLWEAEAGGSPEVRSL RPTWPTW*NPVSTKKTKLSWAWWQAPIIPATIE AEAGELLEPRRRRLQ*AEIAPLHSSLGNKSETPP PGKKKKTTHTHTN*YYTFCRFQK*TQFPGRGSR GCFSCFKS*VGQIVEAGEWGKWLITPLIPALWE AKVGKSLEPGSSRPVWPT*QDPISTKENKS*V*A PGASPGVERSVAYKV*GAPVAL*GDCCRGPSPD MAASPGGARSCRPSGLWKPSLTGSASTPMRTSA WRCRRRTCSHAVAACVGTGESGCASTAGLVLRDP GPQLLSGGDFEDFLGLLRVRSVSGRLRESGRQG LARLLLPGRCLNQGWPGRAV*GVASQQNSQFQK MWWNVWPLVWFVSSHVPFSCLGNQFGAFPYV*LA GMAPP*QQGGGTELSAPAACSLALVPQ*RCSLA AADPKCLRA*KNAWASPLTS*MGITGDSHFREL VVFLSTLGKFSVCSVENQCSTRKGTRSLVCCNGG YPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPP CEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTY KQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAF SVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWG VENGTPYWLVANSWNTDWGDNGFFKILRGQDHC GIESEVVAGIPRTDQYWGRNAFYSLSSRKIQVSD RV*RTGLAKHQTCLPRRPSPGYIPACGYSADRP CEPPLPAQSVLPPVD*CRREYLLPQLTVAPSVI HPSPGSKTETQEWKAEFLTG*KFPHQFPQYLQAS SFPHLSQKSEERRCWEPFGERQSPRPPASIEFA MSQPL*SCAQHDSLIEVLFFRALC*SCG*ASGT AGDLC*FYRLPPYDAAQKETKWSGVVSDPLISTT TRKIV*EKPAFTVFEKLQLHPVKLTRNACANKS FLQLEVYSDGISDPLSLPIDL*LLSLFVPAENH VLELHVLATLGTSS*LLAAPAMFSTMASLPQLVP CHPRQPSL*VPGKLALA*ELKT*LCSIFLLLL* LTE*DSVSQAGVQWRLLSSLQPAAS*IQAILLL QPSE*LG*QALTNMPG*FLYF*VHTGFHHVGQAS FKLPASGGLPASASQSVGITGVSHWALPVFFIS HKSSNKLRIQLIKQAWCLGDLT*PRCYPVGNHI PLDALQPWFGLESGPVYSILNLYATGLHCWSGTL VLCIALV*KHVIAVLGIEQTRRTSNHSTVLPRY TSVLPFPTEVV*G*LFPKAFKFGF*CDSEFRNQ MLDQISSENLRNIVGKVC*ASLKCHDEHNSRPSW LTPVIPALWEAKVGG*QGQEFKTSLANMLKPHL Y*KYKN*LGMVAHACNPSYLGG*GRRIA*TREA EVAVSQDSASALQPR*QRKAPSQ*LKKKKKKKRP GAVAQACNPSTLGG*GGQIT*GQEF*DQPWQHG ETPSLLKIQN*PSMLAHACNPSYSGG*GTRIA* TWEAEDAVSRDHAIALQPGGQE*ICVSPKKKRKR KMLNKGYHKPQIHNHLSFQFQVEYIHNLNKVLP APKLS*FIPCALSPSPPSYTHLQLLACF*HGAS GDVLVSDKELWGMSTSA*GFVRNTCLAVFVTGKE LSVLIHLRSPH*S*LCHLSSIVSPGFLSK*DDR WT*QCLFSLYLASPENHSRIEIRV*VGSPTSAF

Sequence CTSB.kAug05

CTSB.F10 (forward primer)
CTSB.R7 (reverse primer)
Intron retention

Frame 0

Frame 1

Frame 2

Sequence CTSB.tAug05

CACCCCCAGTGTTTTCTATTGTGCTTCTCCAAGGTGTCTCAGCCGCAGATGTGACAGACAAGGTCTGGGTCCCCACTATGTGGGCAGACTCAATCTTGAG TAATTTAAGGGAAATCTAAGAAAAGCCAGATGAGGCCAAGTATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGATGCCAAGGCAAGCGGATCACCTG AGGTCAGGAGTTCGAGACCAGTCTGACCAACATGATGAAACCCCGTCTCTACTAAAAATAAAAAACTTTGCCAGGCGTGGTGGCGGGCGCCTGTAATCCC AGCTACTCGGGAGGCTGAGGCTAGAGAATCGCATGAACCCGGGAGGCAGAGGTTGCGGTGAGCTGAGATGATGCCACTTCCCACGTGAGGATGGGGTAGT TAAGTTGCACGACACTGCCTTCTGCACAATGGGGATGATATGAGAAATGATGAGAGGATCCTGGGCAGAGGTTGAGGCAGGAGAGCAGGGCAAGAAACAC TATCTGGACTGAATAAACTTATTTAAAAATGAGTTTATAATTTGTGGATAAATTCATCTTAAAAAATCCATTACATAAGCCTAGAAAGAAGCGTACAATA TCGCAAGTCAGGCCAGGTGTGGTGGATCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCATTTGAAGCCAGGAGTTTAAGACCAGCC TGGCCAACATGGTGAAACCCTGTCTTTACTAAAAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAC GAGAATCGATTGAACTCAGGAGGCAGAGGTTGCAGTGGGCAGAGATCTCGCCACTGCACTCCAGCCTGGATGACACAGTGAAATTCTGTCCACTCCCACC CTCCCGTCCCACCCAAAAAAAAAATCAAAAGGTGCATTGAGCAACAGAATTTCCTTTTGTTTTATGAAATTACCAAGGACACCTGTCTGCAGCCTTCTTC GGCATGTCTTGTGCTCATTTCCTTGTTAGGGGAGTGTGGCCCAGCCAAAGTGGCCTTTGCAGGGATTGGGGAGGGAGTTGTGGGATCAGAGCTTGTAATG AAGACCTTCCTTTATCCAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGA GATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGG GGGATTCGGGGTGGGGACAACTCTGACAAGGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAAT GATGGCGAGGATTAGGTGGAGTGGCGGGCAGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCC CGTCCGCAAATACCAGGAGCTCTCACTGCTACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAA ATACACGTAACAGAAATGTAATAATCTTAGCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTG TCACCCAGGCTGGAGTGCAGTGGTGGGATATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGA CTACAGGCATGTGCCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTG ATCTGCCTTCCTCGGCCTCGCAAAATGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACAT TCACAGTGTTGCTCAGCCACCACCACCATCTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCC TGGTAAGCCCCACTCTACTTTCTGTCTCTATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTT TTTGTTAAGAAAAAAACACAGCCAGGTACGGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTG AGACCAACCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGG CTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCC TCCAGGAAAAAAAAAAAAAACCACACACACACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGG GGGTGCTTCTCTTGCTTTAAAAGCTGAGTTGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGG CCAAGGTGGGCAAATCACTTGAACCCGGGAGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGC CCCAGGAGCGTCCCCTGGTGTTGAGAGATCAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGAC ATGGCAGCCTCACCCGGTGGGGCTCGTTCCTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGT GGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCC TGGGCCCCAGCTTCTCAGTGGAGGGGACTTTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGC CTGGCAAGGCTGCTGCTTCCTGGGAGGTGCCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAA TGTGGTGGAATGTCTGGCCCCTGGTCTGGTTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGC GGGGATGGCTCCACCTTAACAACAAGGTGGTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCG GCTGCAGATCCGAAATGCCTGAGGGCTTAAAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGG TGGTGTTTTTAAGTACCTTAGGGAAGTTTAGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGGTATGGACATGA ACAGGAGAGCCTCCTGTCTTCTCCCGGATCTTTGTGGGCAGGGGTGGGGCTGGCGGTTATTCCCTGCAAGCTGTGCTTATCTAGGGAGCGTCCCTTGGAG GGTTTGGGGTCTGGGAGGTCTGCTCGCACCACTTGCTGCAGCTGGGGGTGGGTCCACGAGTGGCCTCGGGCACTTGGGTAGCACACAGTGGTCTGGAGAG CTGGTGGTGCTTCTCTCAGAGGTTTTCATTAGAGGCTGTCTTTTCAGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCC TGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGTAAGTGTGTCCCCTTGGCCACTTTCTGGCCAGATGGATTGTTTGAGCAATTAACCATCATGGCTTT ATTTGCCTTTATAAACTGGGGGTTGAGACAGAGGGGCTGCTGAGAGGTGCTAGCCAGGTGCACAGGCCTCTGGCAGGACCTGCCTGGCGTCCATGCTGCA GGCACGAGGCTTGCCTTGCCCCAGGTCTTCTCCGTGGGGGCTGTAGGTTGACTCCGCTTTCTCCCGCGTCCCATCAGGGTGCAGACCGTACTCCATCCCT CCCTGTGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCT ACAAACAGGACAAGCACTACGGTAAGGGGCCTGGGGCCTGGCCACGGCGCACGTGGAGGCTGGGGAGCTGCTGCATCCCTCCTCACGCTGCAGCGAAGAG GTCAGGGCTGAGGAGCCTTGGGGTCCCGGAACTCTAGGATAGAGGAGGGGGAGTGATGCCCTCTTGCCAGGAGAAGCAGCACACTCTCCACTTTCTGCCT GTTCCACCCTGAACTCAGCCTCAGCCCTTCCAAACTGGAAGGGACCAAAGCCCTCCTTTTACAAGGGAGTGGCGTGTCCTGGAGTCTTGAGGTATCCTGG CCTCTTCCGGGGCCTCGTGCTCATTGCTCCGTTCTCCCATTTCTGAGCTTCCAGCCCCAGCCCTGTCCTTAGTTCTTCAGGGGAGCCTTCCTTGAGCTCC CCTGGCAGGGCAAGTCCCCTTAGATGCAGTCTTCCCCTGGAGGCCACCAGAAATCAGTGACTGGCTGACCGTGGCGTGCCACGAGGCACGGTCACTGCCC TCCCCAACCCCGAGCTCGGTTCATTTTCCAGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCG TGGAGGGAGCTTTCTCTGTGTATTCGGACTTCCTGCTCTACAAGTCAGGTGCGTGCTGATGGCTGATGGCAATAGAGGGTGGGGGTCGGGAGGGGAGCCT GGGTGCCAGGATGGTTCATGTTGACCAATAGGGCTGGATTGGGCAGGCAGGTGAGGGGCTGGGGAAGAGGGCTGTGTGGGGCCTTCCATATAGGCTCACT CCTCTGTGGACCAGCCTGGCACCTGACTGTCTTTGTCCAGGTGTTGTGCCAACACACACAATTCCAGAGGCCTCTCCCAGAGCCTCTGGGCTAGTTCCTT CCCATCCATTAGCGTCCAGCTCATACATGTTCTCTGTGGGAACGCCCCCCTCCCTGGTGGCTGGCCACGCCTGCCCTCGCCTTCCTGGCTTTGCATTGCT TTACCAGCATGTTTCTGTTATAATGGCAGGCCGTTCGTGTTTGCACTGGCTGACACATTTTCTTCATCTTTCTCTTTGCAAAGGCCTAGATCGTGCCTCA CACCAGTGGATGAACACACAGGGAGGACCTTGCCCTGGGTAGTGCCATAGGGACTCCAACCAAGGAATCTGGACAGTCCCCATCCCCAAGTCCTGTCTTA GAAATCCCTTGCTCCAGATGAACAGTCTTTGCTGACTGCATCTATCCCATTAATATTTTGCTAGATTGGAAACTTGTAAAACAACAGTTTGACAAAGGAA GATGATTCTGGCAAGAAAAAGTTTCTGTGAAGATGGCACTTCTTAGCATAGTCCTCGCCCTTATTATTAAAAGATGCATTTCCACTTTGACAGTGATTTC CCCACCCGAGAGCCTGGTTGGGCAGCCCTGAGCCCCTTAGCCAGTATCTTTCCCATCAGCTAGCATTAGCTGCCACCAGTCCTCCCTTCTCCTCCCTCAC CTCTTCAGGGTGTACCTGGAGCAGCTATTAAAACTCTTTAAGCCGTGTAACGCTTTTGACAAACCCTTACTTACATGGAATCCCAGTATAAAGAACAGAT GAATCAGTCATCTAGCATTAAATGTTTTATCAATTAGAATGTATTAGAAATTCTATTTTTAAAGCCAACAAGCACATTTTGTAGGAATCTATTTCAATAT TTACTCAACAACTTTGAGTGTGTTGGGCCTTCAAAAATATATTTAATTCCCCAGTATTGTGGGACCCCCCCTGGAGCCTCCTGGTGGAGCAGCAGGTTCC CTCTGGAAGCTGTTTCTCCTTCCCGGAGCATGACCAGCTGAGTGTGGGGGGTGCTGTGGGGCGTGGGACAGTGGCCCGGTCTCGGTCTCAGCCAGTTCTT CCCTTTTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGC TGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGTGAGTGGCTGCCCCCTTCCTGCCAAGAACAGTGAATTGTGAGCCACACCCCGTGGCCATC TCGGCTTTCTCTGTTCTACCCACCGCACAGCCTTAAACCCTGGACCTACGGCCAGGCTGTGAGCTCCTCCTAAGTGCCAGGCAGTCAGGAGTTCCCTTTT GCTGTGAGGGCAGACTCTGAGCAGCTTCAGAGCCAACGCCTGCAACAGTTCCCACAGCATCGCGCCAGATCCTGTGATGGGAAGGGTGACCGGGCAGGGG GCTTGCCCGTGGAGGTGTGCCCCACGGCTCCAGAAGCCTTGTGGTGTTGAGGACTGTGCTAGTGGGCCAACAGCAGGAGTGGCCAGGGATGAGTGACTTA AGGTCTTTTAAGGATGAGTCTGACTATATTGGTTGACCCTTGTCACACTTTAAAAGCACCTTACTTTTTATTCCCAGGCTTCTTTAAAATACTCAGAGGA CAGGATCACTGTGGAATCGAATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGT CCTGGGGGCGAGATCGGGGTAGAAATGCATTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCA GACCTGTCTTCCAAGGAGACCAAGTCCTGGCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTT CCCCCTGTAGACTAGTGCCGTAGGGAGTACCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGG AATGGAAAGCGGAGTTCCTAACAGGATGAAAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGA GAGACGGTGTTGGGAGCCCTTTGGAGAACGCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCAT GATTCTTTAATAGAAGTTTTATTTTTTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTC CTTATGACGCGGCTCAAAAGGAAACCAAGTGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTT TACTGTTTTTGAAAAATTACAGCTTCACCCTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATC TCAGATCCTTTGTCACTGCCTATAGACTTGTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGA CTTCATCTTAACTTCTCGCTGCCCCAGCCATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCC TGGTAAGCTTGCCCTTGCTTAAGAACTCAAAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGA GTGCAGTGGCGCCTTCTCAGCTCACTGCAACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCA CCAATATGCCTGGGTAATTTTTGTATTTTTAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGC CTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTC ATAAAACAGGCTTGGTGTCTTGGTGATCTCACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGG AGTCAGGGCCTGTATACAGTATTTTGAATTTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGT TATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTA TGAGGCTAGCTCTTTCCAAAAGCATTCAAGTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGA ACATTGTAGGAAAGGTTTGTTAAGCATCTCTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC CAAGGTGGGAGGATGACAAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTG GCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCAC TCCAGCCTCGGTGACAGCGCAAGGCTCCGTCTCAATAATT

CTSB.F10 (forward primer)
CTSB.R7 (reverse primer)
Intron retention

Frame 0

HPQCFLLCFSKVSQPQM*QTRSGSPLCGQTQS* VI*GKSKKSQMRPSMVAHACNPSTLGCQGKRIT* GQEFETSLTNMMKPRLY*K*KTLPGVVAGACNP SYSGG*G*RIA*TREAEVAVS*DDATSHVRMG* LSCTTLPSAQWG*YEK**EDPGQRLRQESRARNT IWTE*TYLKMSL*FVDKFILKNPLHKPRKKRTI SQVRPGVVDHACNPSTLGGQGRQII*SQEFKTS LANMVKPCLY*KYKN*PGVVVHACNPSYSGG*GT RID*TQEAEVAVGRDLATALQPG*HSEILSTPT LPSHPKKKSKGALSNRISFCFMKLPRTPVCSLL RHVLCSFPC*GSVAQPKWPLQGLGRELWDQSL** RPSFIQSYVYRGPEAACKLRCTGTMATVSHHQR DQRPGLLWLLLGKALLAGGEALERKWEQHWRVL GDSGWGQL*QGKL*KLSESQFPSVQKSQSLWP*M MARIRWSGGQSIQQIASPRVQVAKQLPFPDMLA RPQIPGALTATLLQESIPLVSVSCLNFVI*LW* NTRNRNVIILAIFFCFLFLSFFFFFFFGDGVLLC HPGWSAVVGYWLTATSASWVQAILLPQSPQ*LR LQACATTPS*FLYF**RWGFAMLDSLVSNASPV DLPSSASQNAGITGVSHRAQQLFSSVQFCSIKYI HSVAQPPPPSVPRTLFQLARQKLCPH*HQYCSP W*APLYFLSL*I*LLGTSYKWITTVFIFWVSCV FC*EKNTARYGSSRL*SQHFGRLRQADHLRSGV* DQPGQHGETLSLLKRQNLAGPGGRHL*SQLL*R LRQENCLNPGGVGCSELRLRHCIPAWATKVKLR LQEKKKKPHTHIQTNTTHFADFRNEPSFQAEVHG GASLALKAELGKSLRQESGASGSSRL*SQHFGR PRWANHLNPGVQDQSGQRSKTPFLPKKIKAEFE PQERPLVLRDQLPTRSEVPLWPFEGTAAEGPART WQPHPVGLVPAGLRGCGSHL*PDLHPHQCARQR GGVGGGPAHMLWQHVWGRVSQAVLPQRV*C*ET LGPSFSVEGTLRTSWDCCESEVFRGDSESLAGRA WQGCCFLGGA*TKAGPAELSEGWHPSKTVSFRK CGGMSGPWSGLCHRMFLFPAWGTSLGHFLMCS* RGWLHLNNKVVAQNFLLQLPAASPWFRSSGVHWR LQIRNA*GLKKMHGLLP*LVKWESLAILISENW WCF*VP*GSLASAQLRISVLPGRALVAWSGMDM NRRASCLLPDLCGQGWGWRLFPASCAYLGSVPWR VWGLGGLLAPLAAAGGGSTSGLGHLGSTQWSGE LVVLLSEVFIRGCLFSCNGGYPAEAWNFWTRKG LVSGGLYESHVGKCVPLATFWPDGLFEQLTIMAL FAFINWGLRQRGC*EVLARCTGLWQDLPGVHAA GTRLALPQVFSVGAVG*LRFLPRPIRVQTVLHP SL*APRQRLPAPMHGGGRYPQV*QDL*AWLQPDL QTGQALR*GAWGLATAHVEAGELLHPSSRCSEE VRAEEPWGPGTLG*RRGSDALLPGEAAHSPLSA CSTLNSASALPNWKGPKPSFYKGVACPGVLRYPG LFRGLVLIAPFSHF*ASSPSPVLSSSGEPSLSS PGRASPLRCSLPLEATRNQ*LADRGVPRGTVTA LPNPELGSFSRIQFLQRLQ*REGHHGRDLQKRPR GGSFLCVFGLPALQVRCVLMADGNRGWGSGGEP GCQDGSC*PIGLDWAGR*GAGEEGCVGPSI*AH SSVDQPGT*LSLSRCCANTHNSRGLSQSLWASSF PSISVQLIHVLCGNAPLPGGWPRLPSPSWLCIA LPACFCYNGRPFVFALADTFSSSFSLQRPRSCL TPVDEHTGRTLPWVVP*GLQPRNLDSPHPQVLS* KSLAPDEQSLLTASIPLIFC*IGNL*NNSLTKE DDSGKKKFL*RWHFLA*SSPLLLKDAFPL*Q*F PHPRAWLGSPEPLSQYLSHQLALAATSPPFSSLT SSGCTWSSY*NSLSRVTLLTNPYLHGIPV*RTD ESVI*H*MFYQLECIRNSIFKANKHIL*ESISI FTQQL*VCWAFKNIFNSPVLWDPPWSLLVEQQVP SGSCFSFPEHDQLSVGGAVGRGTVARSRSQPVL PFSGVYQHVTGEMMGGHAIRILGWGVENGTPYW LVANSWNTDWGDNGEWLPPSCQEQ*IVSHTPWPS RLSLFYPPHSLKPWTYGQAVSSS*VPGSQEFPF AVRADSEQLQSQRLQQFPQHRARSCDGKGDRAG GLPVEVCPTAPEALWC*GLC*WANSRSGQG*VT* GLLRMSLTILVDPCHTLKAPYFLFPGFFKILRG QDHCGIESEVVAGIPRTDQYWEKI*SAVGLSCQ SWGRDRGRNAFYSLSSRKIQVSDRV*RTGLAKHQ TCLPRRPSPGYIPACGYSADRPCEPPLPAQSVL PPVD*CRREYLLPQLTVAPSVIHPSPGSKTETQ EWKAEFLTG*KFPHQFPQYLQASSFPHLSQKSEE RRCWEPFGERQSPRPPASIEFAMSQPL*SCAQH DSLIEVLFFRALC*SCG*ASGTAGDLC*FYRLP PYDAAQKETKWSGVVSDPLISTTTRKIV*EKPAF TVFEKLQLHPVKLTRNACANKSFLQLEVYSDGI SDPLSLPIDL*LLSLFVPAENHVLELHVLATLG TSS*LLAAPAMFSTMASLPQLVPCHPRQPSL*VP GKLALA*ELKT*LCSIFLLLL*LTE*DSVSQAG VQWRLLSSLQPAAS*IQAILLLQPSE*LG*QAL TNMPG*FLYF*VHTGFHHVGQASFKLPASGGLPA SASQSVGITGVSHWALPVFFISHKSSNKLRIQL IKQAWCLGDLT*PRCYPVGNHIPLDALQPWFGL ESGPVYSILNLYATGLHCWSGTLVLCIALV*KHV IAVLGIEQTRRTSNHSTVLPRYTSVLPFPTEVV *G*LFPKAFKFGF*CDSEFRNQMLDQISSENLR NIVGKVC*ASLKCHDEHNSRPSWLTPVIPALWEA KVGG*QGQEFKTSLANMLKPHLY*KYKN*LGMV AHACNPSYLGG*GRRIA*TREAEVAVSQDSASA LQPR*QRKAPSQ*

Frame 1

TPSVFYCASPRCLSRRCDRQGLGPHYVGRLNLE *FKGNLRKAR*GQVWWLTPVIPALWDAKASGSP EVRSSRPV*PT**NPVSTKNKKLCQAWWRAPVIP ATREAEARESHEPGRQRLR*AEMMPLPT*GWGS *VARHCLLHNGDDMRNDERILGRG*GRRAGQET LSGLNKLI*K*VYNLWINSS*KIHYISLERSVQY RKSGQVWWIMPVIPALWEAKAGRSFEARSLRPA WPTW*NPVFTKNTKISRVWWCMPVIPATQEAEA RESIELRRQRLQWAEISPLHSSLDDTVKFCPLPP SRPTQKKNQKVH*ATEFPFVL*NYQGHLSAAFF GMSCAHFLVRGVWPSQSGLCRDWGGSCGIRACN EDLPLSRVMFTEDLKLPASFDAREQWPQCPTIKE IRDQGSCGSCWVRPCWLAGKRWRESGSNTGESW GIRGGDNSDKASYRNFLSPSFHQYKNHNPSGHE *WRGLGGVAGRASSRLQVHVYRWRSSSLSLTCWP VRKYQELSLLLCFKKASL*CQ*AVLILSFNCGK IHVTEM**S*QSSFVFFFFLFFFFFFLEMESCS VTQAGVQWWDIGSLQPLPPGFKQFSCLSLPSS*D YRHVPPRPANFCIFSRDGVLPCWTAWSQTPHLL ICLPRPRKMLGLQA*ATVPSNYFQVYSSVALST FTVLLSHHHHLSPELFFSSQDRNSVPINTNIVAP GKPHSTFCLYEFDS*GPHTSGSQQYLFSG*AVF FVKKKTQPGTVAHACNLNTLGG*GRRIT*GQEF ETNLANMVKPCLY*KDKT*LGLVAGTYNPSYYRG *GRRIA*TQEA*VAVS*DCATAFQPGQQK*NSA SRKKKKNHTHTYKLILHILQISEMNPVSRQRFT GVLLLL*KLSWANR*GRRVGQVAHHASNPSTLGG QGGQIT*TREFKTSLANVARPHFYQRK*KLSLS PRSVPWC*EISCLQGLRCPCGPLRGLLQRAQPG HGSLTRWGSFLQAFGAVEAISDRICIHTNAHVSV EVSAEDLLTCCGSMCGDG*VRLCFHSGFSAERP WAPASQWRGL*GLPGTAASQKCFGETPRVWQAG PGKAAASWEVPEPRLARQSCLRGGIPAKQSVSEN VVECLAPGLVCVIACSFFLLGEPVWGISLCVVS GDGSTLTTRWWHRTFCSSCLQPRLGSAVAVFTG GCRSEMPEGLKKCMGFSPN*LNGNHWRFSFQRTG GVFKYLREV*RLLS*ESVFYPEGHS*PGLVWT* TGEPPVFSRIFVGRGGAGGYSLQAVLI*GASLG GFGVWEVCSHHLLQLGVGPRVASGTWVAHSGLES WWCFSQRFSLEAVFSAVMVAILLKLGTSGQEKA WFLVASMNPM*VSVSPWPLSGQMDCLSN*PSWL YLPL*TGG*DRGAAERC*PGAQASGRTCLASMLQ ARGLPCPRSSPWGL*VDSAFSRVPSGCRPYSIP PCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPT YKQDKHYGKGPGAWPRRTWRLGSCCIPPHAAAKR SGLRSLGVPEL*DRGGGVMPSCQEKQHTLHFLP VPP*TQPQPFQTGRDQSPPFTREWRVLES*GIL ASSGASCSLLRSPISELPAPALSLVLQGSLP*AP LAGQVPLDAVFPWRPPEISDWLTVACHEARSLP SPTPSSVHFPGYNSYSVSNSEKDIMAEIYKNGP VEGAFSVYSDFLLYKSGAC*WLMAIEGGGREGSL GARMVHVDQ*GWIGQAGEGLGKRAVWGLPYRLT PLWTSLAPDCLCPGVVPTHTIPEASPRASGLVP SHPLASSSYMFSVGTPPSLVAGHACPRLPGFALL YQHVSVIMAGRSCLHWLTHFLHLSLCKGLDRAS HQWMNTQGGPCPG*CHRDSNQGIWTVPIPKSCL RNPLLQMNSLC*LHLSH*YFARLETCKTTV*QRK MILARKSFCEDGTS*HSPRPYY*KMHFHFDSDF PTREPGWAALSPLASIFPIS*H*LPPVLPSPPS PLQGVPGAAIKTL*AV*RF*QTLTYMESQYKEQM NQSSSIKCFIN*NVLEILFLKPTSTFCRNLFQY LLNNFECVGPSKIYLIPQYCGTPPGASWWSSRF PLEAVSPSRSMTS*VWGVLWGVGQWPGLGLSQFF PFQECTNTSPER*WVAMPSASWAGEWRMAHPTG WLPTPGTLTGVTMVSGCPLPAKNSEL*ATPRGH LGFLCSTHRTALNPGPTARL*APPKCQAVRSSLL L*GQTLSSFRANACNSSHSIAPDPVMGRVTGQG ACPWRCAPRLQKPCGVEDCASGPTAGVARDE*L KVF*G*V*LYWLTLVTL*KHLTFYSQASLKYSED RITVESNQKWWLEFHAPISTGKRSNLPWACRAS PGGEIGVEMHFIL*VHVRYKFQTGSEGLDWPNI RPVFQGDQVLATSQPVVTVQTGHVSHRCQHRASF PL*TSAVGSTCCPS*LWPPP*SIHLQGARQRRR NGKRSS*QDESSPISSPSTSKQVAFHICHRNQR RDGVGSPLENASLPGPLHLSSLQCHNLSDLVLSM IL**KFYFFVHSANHVGEPVEQRETCASFTDCL LMTRLKRKPSGQELFLTH*SLLPQGK*FRRNQL LLFLKNYSFTLSS*QGMPVPIKVFSNLKSTLMGS QILCHCL*TCSCCLSLSLQRITSWNCMFLRLLG LHLNFSLPQPCFQPWHPSPN*FPVILVNLLCKC LVSLPLLKNSKHSCALFFCCCCD*QSEIPSPRLE CSGAFSAHCNLQPPRFKRFSCFSLPSSWDDRHS PICLGNFCIFKYIQDFTMLARLVSNSRPQVVCL PQPPKVLGLQA*ATGPCLYFLSATNPATS*GFSS *NRLGVLVISHNQDATPWGTTSPWMPSSLGLGW SQGLYTVF*ICMPLVCIAGQEL*CFA*PWFRNM L*QFLV*SKLEEPAIIPLSCQGTPQYSPSQLKWY EASSFQKHSSLASDVTQNLGTRC*IK*ALKI*G TL*ERFVKHLLSAMMSITAGRRGSRL*SQHFGR PRWEDDKVRSSRPAWPTC*NLTSTKNTKISWAWW HMPVIPATWEAEAGESLEPGRRRLQ*AKTVPVH SSLGDSARLRLNN

Frame 2

PPVFSIVLLQGVSAADVTDKVWVPTMWADSILS NLREI*EKPDEAKYGGSRL*SQHFGMPRQADHL RSGVRDQSDQHDETPSLLKIKNFARRGGGRL*S QLLGRLRLENRMNPGGRGCGELR*CHFPREDGVV KLHDTAFCTMGMI*EMMRGSWAEVEAGEQGKKH YLD*INLFKNEFIICG*IHLKKSIT*A*KEAYN IASQARCGGSCL*SQHFGRPRQADHLKPGV*DQP GQHGETLSLLKIQKLAGCGGACL*SQLLRRLRH ENRLNSGGRGCSGQRSRHCTPAWMTQ*NSVHSH PPVPPKKKIKRCIEQQNFLLFYEITKDTCLQPSS ACLVLISLLGECGPAKVAFAGIGEGVVGSELVM KTFLYPELCLPRT*SCLQASMHGNNGHSVPPSK RSETRAPVAPAG*GPAGWRGSAGEKVGATLESLG GFGVGTTLTRQVIETF*VPVSISTKITIPLAMN DGED*VEWRAEHPADCKSTCTGGEAAPFP*HAG PSANTRSSHCYSASRKHPFSVSELS*FCHLIVVK YT*QKCNNLSNLLLFSFSFFFFFFFFWRWSLAL SPRLECSGGILAHCNLCLLGSSNSPASVSPVAK TTGMCHHAQLIFVFLVEMGFCHVGQPGLKRLTC* SAFLGLAKCWDYRREPPCPATIFKCTVL*H*VH SQCCSATTTICPPNSFSARKTETLSPLTPIL*P LVSPTLLSVSMNLTPRDLIQVDHNSIYFLGELCF LLRKKHSQVR*LTPVISTLWEAEAGGSPEVRSL RPTWPTW*NPVSTKKTKLSWAWWQAPIIPATIE AEAGELLEPRRRRLQ*AEIAPLHSSLGNKSETPP PGKKKKTTHTHTN*YYTFCRFQK*TQFPGRGSR GCFSCFKS*VGQIVEAGEWGKWLITPLIPALWE AKVGKSLEPGSSRPVWPT*QDPISTKENKS*V*A PGASPGVERSVAYKV*GAPVAL*GDCCRGPSPD MAASPGGARSCRPSGLWKPSLTGSASTPMRTSA WRCRRRTCSHAVAACVGTGESGCASTAGLVLRDP GPQLLSGGDFEDFLGLLRVRSVSGRLRESGRQG LARLLLPGRCLNQGWPGRAV*GVASQQNSQFQK MWWNVWPLVWFVSSHVPFSCLGNQFGAFPYV*LA GMAPP*QQGGGTELSAPAACSLALVPQ*RCSLA AADPKCLRA*KNAWASPLTS*MGITGDSHFREL VVFLSTLGKFSVCSVENQCSTRKGTRSLVWYGHE QESLLSSPGSLWAGVGLAVIPCKLCLSRERPLE GLGSGRSARTTCCSWGWVHEWPRALG*HTVVWR AGGASLRGFH*RLSFQL*WWLSC*SLELLDKKRP GFWWPL*IPCR*VCPLGHFLARWIV*AINHHGF ICLYKLGVETEGLLRGASQVHRPLAGPAWRPCC RHEACLAPGLLRGGCRLTPLSPASHQGADRTPSL PVSTTSTAPGPHARGREIPPSVARSVSLATARP TNRTSTTVRGLGPGHGARGGWGAAASLLTLQRR GQG*GALGSRNSRIEEGE*CPLARRSSTLSTFCL FHPELSLSPSKLEGTKALLLQGSGVSWSLEVSW PLPGPRAHCSVLPFLSFQPQPCP*FFRGAFLEL PWQGKSP*MQSSPGGHQKSVTG*PWRATRHGHCP PQPRARFIFQDTIPTASPIARRTSWPRSTKTAP WRELSLCIRTSCSTSQVRADG*WQ*RVGVGRGA WVPGWFMLTNRAGLGRQVRGWGRGLCGAFHIGSL LCGPAWHLTVFVQVLCQHTQFQRPLPEPLG*FL PIH*RPAHTCSLWERPPPWWLATPALAFLALHC FTSMFLL*WQAVRVCTG*HIFFIFLFAKA*IVPH TSG*THREDLALGSAIGTPTKESGQSPSPSPVL EIPCSR*TVFADCIYPINILLDWKLVKQQFDKG R*FWQEKVSVKMALLSIVLALIIKRCISTLTVIS PPESLVGQP*AP*PVSFPSASISCHQSSLLLPH LFRVYLEQLLKLFKPCNAFDKPLLTWNPSIKNR *ISHLALNVLSIRMY*KFYF*SQQAHFVGIYFNI YSTTLSVLGLQKYI*FPSIVGPPLEPPGGAAGS LWKLFLLPGA*PAECGGCCGAWDSGPVSVSASS SLFRSVPTRHRRDDGWPCHPHPGLGSGEWHTLLA GCQLLEH*LG*QW*VAAPFLPRTVNCEPHPVAI SAFSVLPTAQP*TLDLRPGCELLLSARQSGVPF CCEGRL*AASEPTPATVPTASRQIL*WEG*PGRG LARGGVPHGSRSLVVLRTVLVGQQQEWPGMSDL RSFKDESDYIG*PLSHFKSTLLFIPRLL*NTQR TGSLWNRIRSGGWNSTHRSVLGKDLICRGPVVPV LGARSG*KCILFFKFT*DTSFRQGLKDWIGQTS DLSSKETKSWLHPSLWLQCRQAM*ATAASTERP SPCRLVP*GVPAAPADCGPLRDPSISREQDRDAG MESGVPNRMKVPPSVPPVPPSK*LSTFVTEIRG ETVLGALWRTPVSQAPCIYRVCNVTTSLILCSA *FFNRSFIFSCTLLIMWVSQWNSGRPVLVLQIAS L*RGSKGNQVVRSCF*PTDLYYHKENSLGETSF YCF*KITASPCQVNKECLCQ*KFSPT*SLL*WD LRSFVTAYRLVAAVSLCPCRESRPGTACSCDSWD FILTSRCPSHVFNHGIPPPISSLSSSSTFSVSA W*ACPCLRTQNIAVLYFFVVVVTDRVRFRLPGW SAVAPSQLTATCSLLDSSDSPASAFRVAGMTGTH QYAWVIFVFLSTYRISPCWPG*FQTPGLRWSAC LSLPKCWDYRREPLGPACIFYQPQIQQQAEDSA HKTGLVSW*SHITKMLPRGEPHPPGCPPALVWAG VRACIQYFEFVCHWFALLVRNSSALHSPGLETC YSSSWYRAN*KNQQSFHCPAKVHLSTPLPN*SG MRLALSKSIQVWLLM*LRI*EPDARSNKL*KSEE HCRKGLLSIS*VP**A*QPAVVAHACNPSTLGG QGGRMTRSGVQDQPGQHAETSPLLKIQKLAGHG GTCL*SQLLGRLRQENRLNPGGGGCSEPRQCQCT PASVTAQGSVSII

Sequence CTSB.shed4.aAug05

CACCCCCAGTGTTTTCTATTGTGCTTCTCCAAGGTGTCTCAGCCGCAGATGTGACAGACAAGGTCTGGGTCCCCACTATGTGGGCAGACTCAATCTTGAG TAATTTAAGGGAAATCTAAGAAAAGCCAGATGAGGCCAAGTATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGATGCCAAGGCAAGCGGATCACCTG AGGTCAGGAGTTCGAGACCAGTCTGACCAACATGATGAAACCCCGTCTCTACTAAAAATAAAAAACTTTGCCAGGCGTGGTGGCGGGCGCCTGTAATCCC AGCTACTCGGGAGGCTGAGGCTAGAGAATCGCATGAACCCGGGAGGCAGAGGTTGCGGTGAGCTGAGATGATGCCACTTCCCACGTGAGGATGGGGTAGT TAAGTTGCACGACACTGCCTTCTGCACAATGGGGATGATATGAGAAATGATGAGAGGATCCTGGGCAGAGGTTGAGGCAGGAGAGCAGGGCAAGAAACAC TATCTGGACTGAATAAACTTATTTAAAAATGAGTTTATAATTTGTGGATAAATTCATCTTAAAAAATCCATTACATAAGCCTAGAAAGAAGCGTACAATA TCGCAAGTCAGGCCAGGTGTGGTGGATCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCATTTGAAGCCAGGAGTTTAAGACCAGCC TGGCCAACATGGTGAAACCCTGTCTTTACTAAAAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAC GAGAATCGATTGAACTCAGGAGGCAGAGGTTGCAGTGGGCAGAGATCTCGCCACTGCACTCCAGCCTGGATGACACAGTGAAATTCTGTCCACTCCCACC CTCCCGTCCCACCCAAAAAAAAAATCAAAAGGTGCATTGAGCAACAGAATTTCCTTTTGTTTTATGAAATTACCAAGGACACCTGTCTGCAGCCTTCTTC GGCATGTCTTGTGCTCATTTCCTTGTTAGGGGAGTGTGGCCCAGCCAAAGTGGCCTTTGCAGGGATTGGGGAGGGAGTTGTGGGATCAGAGCTTGTAATG AAGACCTTCCTTTATCCAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGA GATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGG GGGATTCGGGGTGGGGACAACTCTGACAAGGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAAT GATGGCGAGGATTAGGTGGAGTGGCGGGCAGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCC CGTCCGCAAATACCAGGAGCTCTCACTGCTACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAA ATACACGTAACAGAAATGTAATAATCTTAGCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTG TCACCCAGGCTGGAGTGCAGTGGTGGGATATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGA CTACAGGCATGTGCCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTG ATCTGCCTTCCTCGGCCTCGCAAAATGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACAT TCACAGTGTTGCTCAGCCACCACCACCATCTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCC TGGTAAGCCCCACTCTACTTTCTGTCTCTATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTT TTTGTTAAGAAAAAAACACAGCCAGGTACGGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTG AGACCAACCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGG CTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCC TCCAGGAAAAAAAAAAAAAACCACACACACACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGG GGGTGCTTCTCTTGCTTTAAAAGCTGAGTTGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGG CCAAGGTGGGCAAATCACTTGAACCCGGGAGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGC CCCAGGAGCGTCCCCTGGTGTTGAGAGATCAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGAC ATGGCAGCCTCACCCGGTGGGGCTCGTTCCTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGT GGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCC TGGGCCCCAGCTTCTCAGTGGAGGGGACTTTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGC CTGGCAAGGCTGCTGCTTCCTGGGAGGTGCCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAA TGTGGTGGAATGTCTGGCCCCTGGTCTGGTTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGC GGGGATGGCTCCACCTTAACAACAAGGTGGTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCG GCTGCAGATCCGAAATGCCTGAGGGCTTAAAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGG TGGTGTTTTTAAGTACCTTAGGGAAGTTTAGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGGTATGGACATGA ACAGGAGAGCCTCCTGTCTTCTCCCGGATCTTTGTGGGCAGGGGTGGGGCTGGCGGTTATTCCCTGCAAGCTGTGCTTATCTAGGGAGCGTCCCTTGGAG GGTTTGGGGTCTGGGAGGTCTGCTCGCACCACTTGCTGCAGCTGGGGGTGGGTCCACGAGTGGCCTCGGGCACTTGGGTAGCACACAGTGGTCTGGAGAG CTGGTGGTGCTTCTCTCAGAGGTTTTCATTAGAGGCTGTCTTTTCAGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCC TGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGTAAGTGTGTCCCCTTGGCCACTTTCTGGCCAGATGGATTGTTTGAGCAATTAACCATCATGGCTTT ATTTGCCTTTATAAACTGGGGGTTGAGACAGAGGGGCTGCTGAGAGGTGCTAGCCAGGTGCACAGGCCTCTGGCAGGACCTGCCTGGCGTCCATGCTGCA GGCACGAGGCTTGCCTTGCCCCAGGTCTTCTCCGTGGGGGCTGTAGGTTGACTCCGCTTTCTCCCGCGTCCCATCAGGGTGCAGACCGTACTCCATCCCT CCCTGTGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCT ACAAACAGGACAAGCACTACGGTAAGGGGCCTGGGGCCTGGCCACGGCGCACGTGGAGGCTGGGGAGCTGCTGCATCCCTCCTCACGCTGCAGCGAAGAG GTCAGGGCTGAGGAGCCTTGGGGTCCCGGAACTCTAGGATAGAGGAGGGGGAGTGATGCCCTCTTGCCAGGAGAAGCAGCACACTCTCCACTTTCTGCCT GTTCCACCCTGAACTCAGCCTCAGCCCTTCCAAACTGGAAGGGACCAAAGCCCTCCTTTTACAAGGGAGTGGCGTGTCCTGGAGTCTTGAGGTATCCTGG CCTCTTCCGGGGCCTCGTGCTCATTGCTCCGTTCTCCCATTTCTGAGCTTCCAGCCCCAGCCCTGTCCTTAGTTCTTCAGGGGAGCCTTCCTTGAGCTCC CCTGGCAGGGCAAGTCCCCTTAGATGCAGTCTTCCCCTGGAGGCCACCAGAAATCAGTGACTGGCTGACCGTGGCGTGCCACGAGGCACGGTCACTGCCC TCCCCAACCCCGAGCTCGGTTCATTTTCCAGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCG TGGAGGGAGCTTTCTCTGTGTATTCGGACTTCCTGCTCTACAAGTCAGGTGCGTGCTGATGGCTGATGGCAATAGAGGGTGGGGGTCGGGAGGGGAGCCT GGGTGCCAGGATGGTTCATGTTGACCAATAGGGCTGGATTGGGCAGGCAGGTGAGGGGCTGGGGAAGAGGGCTGTGTGGGGCCTTCCATATAGGCTCACT CCTCTGTGGACCAGCCTGGCACCTGACTGTCTTTGTCCAGGTGTTGTGCCAACACACACAATTCCAGAGGCCTCTCCCAGAGCCTCTGGGCTAGTTCCTT CCCATCCATTAGCGTCCAGCTCATACATGTTCTCTGTGGGAACGCCCCCCTCCCTGGTGGCTGGCCACGCCTGCCCTCGCCTTCCTGGCTTTGCATTGCT TTACCAGCATGTTTCTGTTATAATGGCAGGCCGTTCGTGTTTGCACTGGCTGACACATTTTCTTCATCTTTCTCTTTGCAAAGGCCTAGATCGTGCCTCA CACCAGTGGATGAACACACAGGGAGGACCTTGCCCTGGGTAGTGCCATAGGGACTCCAACCAAGGAATCTGGACAGTCCCCATCCCCAAGTCCTGTCTTA GAAATCCCTTGCTCCAGATGAACAGTCTTTGCTGACTGCATCTATCCCATTAATATTTTGCTAGATTGGAAACTTGTAAAACAACAGTTTGACAAAGGAA GATGATTCTGGCAAGAAAAAGTTTCTGTGAAGATGGCACTTCTTAGCATAGTCCTCGCCCTTATTATTAAAAGATGCATTTCCACTTTGACAGTGATTTC CCCACCCGAGAGCCTGGTTGGGCAGCCCTGAGCCCCTTAGCCAGTATCTTTCCCATCAGCTAGCATTAGCTGCCACCAGTCCTCCCTTCTCCTCCCTCAC CTCTTCAGGGTGTACCTGGAGCAGCTATTAAAACTCTTTAAGCCGTGTAACGCTTTTGACAAACCCTTACTTACATGGAATCCCAGTATAAAGAACAGAT GAATCAGTCATCTAGCATTAAATGTTTTATCAATTAGAATGTATTAGAAATTCTATTTTTAAAGCCAACAAGCACATTTTGTAGGAATCTATTTCAATAT TTACTCAACAACTTTGAGTGTGTTGGGCCTTCAAAAATATATTTAATTCCCCAGTATTGTGGGACCCCCCCTGGAGCCTCCTGGTGGAGCAGCAGGTTCC CTCTGGAAGCTGTTTCTCCTTCCCGGAGCATGACCAGCTGAGTGTGGGGGGTGCTGTGGGGCGTGGGACAGTGGCCCGGTCTCGGTCTCAGCCAGTTCTT CCCTTTTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGC TGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGTGAGTGGCTGCCCCCTTCCTGCCAAGAACAGTGAATTGTGAGCCACACCCCGTGGCCATC TCGGCTTTCTCTGTTCTACCCACCGCACAGCCTTAAACCCTGGACCTACGGCCAGGCTGTGAGCTCCTCCTAAGTGCCAGGCAGTCAGGAGTTCCCTTTT GCTGTGAGGGCAGACTCTGAGCAGCTTCAGAGCCAACGCCTGCAACAGTTCCCACAGCATCGCGCCAGATCCTGTGATGGGAAGGGTGACCGGGCAGGGG GCTTGCCCGTGGAGGTGTGCCCCACGGCTCCAGAAGCCTTGTGGTGTTGAGGACTGTGCTAGTGGGCCAACAGCAGGAGTGGCCAGGGATGAGTGACTTA AGGTCTTTTAAGGATGAGTCTGACTATATTGGTTGACCCTTGTCACACTTTAAAAGCACCTTACTTTTTATTCCCAGGCTTCTTTAAAATACTCAGAGGA CAGGATCACTGTGGAATCGAATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGT CCTGGGGGCGAGATCGGGGTAGAAATGCATTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCA GACCTGTCTTCCAAGGAGACCAAGTCCTGGCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTT CCCCCTGTAGACTAGTGCCGTAGGGAGTACCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGG AATGGAAAGCGGAGTTCCTAACAGGATGAAAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGA GAGACGGTGTTGGGAGCCCTTTGGAGAACGCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCAT GATTCTTTAATAGAAGTTTTATTTTTTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTC CTTATGACGCGGCTCAAAAGGAAACCAAGTGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTT TACTGTTTTTGAAAAATTACAGCTTCACCCTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATC TCAGATCCTTTGTCACTGCCTATAGACTTGTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGA CTTCATCTTAACTTCTCGCTGCCCCAGCCATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCC TGGTAAGCTTGCCCTTGCTTAAGAACTCAAAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGTGCACT CCAGCCTCGGTGACAGCGCAAGGCTCCGTCTCAATAATTAAAAAAAAAAAAAAAAAAAAAAAGGCCGGGCGCAGTGGCTCAAGCCTGTAATCCCAGCACT TTGGGAGGCTGAGGCGGGCAGATCACCTGAGGTCAGGAGTTTTGAGATCAGCCTTGGCAACACGGTGAAACCCCATCTCTACTAAAAATACAAAATTAGC CAAGCATGCTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGTACGAGAATCGCTTGAACCTGGGAGGCAGAGGATGCAGTGAGCCGAGATCA CGCCATTGCACTCCAGCCTGGGGGACAAGAGTGAATCTGTGTCTCACCAAAAAAAAAAAGAAAAAGAAAGATGCTTAACAAAGGTTACCATAAGCCACAA ATTCATAACCACTTATCCTTCCAGTTTCAAGTAGAATATATTCATAACCTCAATAAAGTTCTCCCTGCTCCCAAACTGAGTTGATTCATTCCATGCGCAC TGTCTCCCTCACCTCCATCGTATACGCACCTGCAGCTCCTTGCCTGCTTCTAGCATGGAGCCAGCGGGGATGTGCTGGTGAGTGACAAGGAATTGTGGGG CATGTCGACATCAGCTTAAGGCTTTGTTAGAAACACCTGCCTTGCAGTGTTTGTGACTGGGAAAGAGCTCAGTGTTTTAATACACCTCAGATCACCACAT TGATCATAACTTTGTCACCTCAGTTCAATAGTTTCTCCTGGATTCTTGTCCAAGTGAGATGACAGGTGGACGTAACAATGCCTTTTCAGTCTTTACCTAG CCTCTCCAGAGAATCACTCCAGAATAGAAATTAGAGTATAGGTAGGCAGTCCAACCTCTGCATTT

CTSB.F10 (forward primer)
CTSB.R7 (reverse primer)
Intron retention

Frame 0

HPQCFLLCFSKVSQPQM*QTRSGSPLCGQTQS* VI*GKSKKSQMRPSMVAHACNPSTLGCQGKRIT* GQEFETSLTNMMKPRLY*K*KTLPGVVAGACNP SYSGG*G*RIA*TREAEVAVS*DDATSHVRMG* LSCTTLPSAQWG*YEK**EDPGQRLRQESRARNT IWTE*TYLKMSL*FVDKFILKNPLHKPRKKRTI SQVRPGVVDHACNPSTLGGQGRQII*SQEFKTS LANMVKPCLY*KYKN*PGVVVHACNPSYSGG*GT RID*TQEAEVAVGRDLATALQPG*HSEILSTPT LPSHPKKKSKGALSNRISFCFMKLPRTPVCSLL RHVLCSFPC*GSVAQPKWPLQGLGRELWDQSL** RPSFIQSYVYRGPEAACKLRCTGTMATVSHHQR DQRPGLLWLLLGKALLAGGEALERKWEQHWRVL GDSGWGQL*QGKL*KLSESQFPSVQKSQSLWP*M MARIRWSGGQSIQQIASPRVQVAKQLPFPDMLA RPQIPGALTATLLQESIPLVSVSCLNFVI*LW* NTRNRNVIILAIFFCFLFLSFFFFFFFGDGVLLC HPGWSAVVGYWLTATSASWVQAILLPQSPQ*LR LQACATTPS*FLYF**RWGFAMLDSLVSNASPV DLPSSASQNAGITGVSHRAQQLFSSVQFCSIKYI HSVAQPPPPSVPRTLFQLARQKLCPH*HQYCSP W*APLYFLSL*I*LLGTSYKWITTVFIFWVSCV FC*EKNTARYGSSRL*SQHFGRLRQADHLRSGV* DQPGQHGETLSLLKRQNLAGPGGRHL*SQLL*R LRQENCLNPGGVGCSELRLRHCIPAWATKVKLR LQEKKKKPHTHIQTNTTHFADFRNEPSFQAEVHG GASLALKAELGKSLRQESGASGSSRL*SQHFGR PRWANHLNPGVQDQSGQRSKTPFLPKKIKAEFE PQERPLVLRDQLPTRSEVPLWPFEGTAAEGPART WQPHPVGLVPAGLRGCGSHL*PDLHPHQCARQR GGVGGGPAHMLWQHVWGRVSQAVLPQRV*C*ET LGPSFSVEGTLRTSWDCCESEVFRGDSESLAGRA WQGCCFLGGA*TKAGPAELSEGWHPSKTVSFRK CGGMSGPWSGLCHRMFLFPAWGTSLGHFLMCS* RGWLHLNNKVVAQNFLLQLPAASPWFRSSGVHWR LQIRNA*GLKKMHGLLP*LVKWESLAILISENW WCF*VP*GSLASAQLRISVLPGRALVAWSGMDM NRRASCLLPDLCGQGWGWRLFPASCAYLGSVPWR VWGLGGLLAPLAAAGGGSTSGLGHLGSTQWSGE LVVLLSEVFIRGCLFSCNGGYPAEAWNFWTRKG LVSGGLYESHVGKCVPLATFWPDGLFEQLTIMAL FAFINWGLRQRGC*EVLARCTGLWQDLPGVHAA GTRLALPQVFSVGAVG*LRFLPRPIRVQTVLHP SL*APRQRLPAPMHGGGRYPQV*QDL*AWLQPDL QTGQALR*GAWGLATAHVEAGELLHPSSRCSEE VRAEEPWGPGTLG*RRGSDALLPGEAAHSPLSA CSTLNSASALPNWKGPKPSFYKGVACPGVLRYPG LFRGLVLIAPFSHF*ASSPSPVLSSSGEPSLSS PGRASPLRCSLPLEATRNQ*LADRGVPRGTVTA LPNPELGSFSRIQFLQRLQ*REGHHGRDLQKRPR GGSFLCVFGLPALQVRCVLMADGNRGWGSGGEP GCQDGSC*PIGLDWAGR*GAGEEGCVGPSI*AH SSVDQPGT*LSLSRCCANTHNSRGLSQSLWASSF PSISVQLIHVLCGNAPLPGGWPRLPSPSWLCIA LPACFCYNGRPFVFALADTFSSSFSLQRPRSCL TPVDEHTGRTLPWVVP*GLQPRNLDSPHPQVLS* KSLAPDEQSLLTASIPLIFC*IGNL*NNSLTKE DDSGKKKFL*RWHFLA*SSPLLLKDAFPL*Q*F PHPRAWLGSPEPLSQYLSHQLALAATSPPFSSLT SSGCTWSSY*NSLSRVTLLTNPYLHGIPV*RTD ESVI*H*MFYQLECIRNSIFKANKHIL*ESISI FTQQL*VCWAFKNIFNSPVLWDPPWSLLVEQQVP SGSCFSFPEHDQLSVGGAVGRGTVARSRSQPVL PFSGVYQHVTGEMMGGHAIRILGWGVENGTPYW LVANSWNTDWGDNGEWLPPSCQEQ*IVSHTPWPS RLSLFYPPHSLKPWTYGQAVSSS*VPGSQEFPF AVRADSEQLQSQRLQQFPQHRARSCDGKGDRAG GLPVEVCPTAPEALWC*GLC*WANSRSGQG*VT* GLLRMSLTILVDPCHTLKAPYFLFPGFFKILRG QDHCGIESEVVAGIPRTDQYWEKI*SAVGLSCQ SWGRDRGRNAFYSLSSRKIQVSDRV*RTGLAKHQ TCLPRRPSPGYIPACGYSADRPCEPPLPAQSVL PPVD*CRREYLLPQLTVAPSVIHPSPGSKTETQ EWKAEFLTG*KFPHQFPQYLQASSFPHLSQKSEE RRCWEPFGERQSPRPPASIEFAMSQPL*SCAQH DSLIEVLFFRALC*SCG*ASGTAGDLC*FYRLP PYDAAQKETKWSGVVSDPLISTTTRKIV*EKPAF TVFEKLQLHPVKLTRNACANKSFLQLEVYSDGI SDPLSLPIDL*LLSLFVPAENHVLELHVLATLG TSS*LLAAPAMFSTMASLPQLVPCHPRQPSL*VP GKLALA*ELKT*LCSIFLLLL*LTE*DSVSQCT PASVTAQGSVSIIKKKKKKKKAGRSGSSL*SQH FGRLRRADHLRSGVLRSALATR*NPISTKNTKLA KHAGTCL*SQLLGRLRYENRLNLGGRGCSEPRS RHCTPAWGTRVNLCLTKKKKKKKDA*QRLP*AT NS*PLILPVSSRIYS*PQ*SSPCSQTELIHSMRT VSLTSIVYAPAAPCLLLAWSQRGCAGE*QGIVG HVDISLRLC*KHLPCSVCDWERAQCFNTPQITT LIITLSPQFNSFSWILVQVR*QVDVTMPFQSLPS LSRESLQNRN*SIGRQSNLCI

Frame 1

TPSVFYCASPRCLSRRCDRQGLGPHYVGRLNLE *FKGNLRKAR*GQVWWLTPVIPALWDAKASGSP EVRSSRPV*PT**NPVSTKNKKLCQAWWRAPVIP ATREAEARESHEPGRQRLR*AEMMPLPT*GWGS *VARHCLLHNGDDMRNDERILGRG*GRRAGQET LSGLNKLI*K*VYNLWINSS*KIHYISLERSVQY RKSGQVWWIMPVIPALWEAKAGRSFEARSLRPA WPTW*NPVFTKNTKISRVWWCMPVIPATQEAEA RESIELRRQRLQWAEISPLHSSLDDTVKFCPLPP SRPTQKKNQKVH*ATEFPFVL*NYQGHLSAAFF GMSCAHFLVRGVWPSQSGLCRDWGGSCGIRACN EDLPLSRVMFTEDLKLPASFDAREQWPQCPTIKE IRDQGSCGSCWVRPCWLAGKRWRESGSNTGESW GIRGGDNSDKASYRNFLSPSFHQYKNHNPSGHE *WRGLGGVAGRASSRLQVHVYRWRSSSLSLTCWP VRKYQELSLLLCFKKASL*CQ*AVLILSFNCGK IHVTEM**S*QSSFVFFFFLFFFFFFLEMESCS VTQAGVQWWDIGSLQPLPPGFKQFSCLSLPSS*D YRHVPPRPANFCIFSRDGVLPCWTAWSQTPHLL ICLPRPRKMLGLQA*ATVPSNYFQVYSSVALST FTVLLSHHHHLSPELFFSSQDRNSVPINTNIVAP GKPHSTFCLYEFDS*GPHTSGSQQYLFSG*AVF FVKKKTQPGTVAHACNLNTLGG*GRRIT*GQEF ETNLANMVKPCLY*KDKT*LGLVAGTYNPSYYRG *GRRIA*TQEA*VAVS*DCATAFQPGQQK*NSA SRKKKKNHTHTYKLILHILQISEMNPVSRQRFT GVLLLL*KLSWANR*GRRVGQVAHHASNPSTLGG QGGQIT*TREFKTSLANVARPHFYQRK*KLSLS PRSVPWC*EISCLQGLRCPCGPLRGLLQRAQPG HGSLTRWGSFLQAFGAVEAISDRICIHTNAHVSV EVSAEDLLTCCGSMCGDG*VRLCFHSGFSAERP WAPASQWRGL*GLPGTAASQKCFGETPRVWQAG PGKAAASWEVPEPRLARQSCLRGGIPAKQSVSEN VVECLAPGLVCVIACSFFLLGEPVWGISLCVVS GDGSTLTTRWWHRTFCSSCLQPRLGSAVAVFTG GCRSEMPEGLKKCMGFSPN*LNGNHWRFSFQRTG GVFKYLREV*RLLS*ESVFYPEGHS*PGLVWT* TGEPPVFSRIFVGRGGAGGYSLQAVLI*GASLG GFGVWEVCSHHLLQLGVGPRVASGTWVAHSGLES WWCFSQRFSLEAVFSAVMVAILLKLGTSGQEKA WFLVASMNPM*VSVSPWPLSGQMDCLSN*PSWL YLPL*TGG*DRGAAERC*PGAQASGRTCLASMLQ ARGLPCPRSSPWGL*VDSAFSRVPSGCRPYSIP PCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPT YKQDKHYGKGPGAWPRRTWRLGSCCIPPHAAAKR SGLRSLGVPEL*DRGGGVMPSCQEKQHTLHFLP VPP*TQPQPFQTGRDQSPPFTREWRVLES*GIL ASSGASCSLLRSPISELPAPALSLVLQGSLP*AP LAGQVPLDAVFPWRPPEISDWLTVACHEARSLP SPTPSSVHFPGYNSYSVSNSEKDIMAEIYKNGP VEGAFSVYSDFLLYKSGAC*WLMAIEGGGREGSL GARMVHVDQ*GWIGQAGEGLGKRAVWGLPYRLT PLWTSLAPDCLCPGVVPTHTIPEASPRASGLVP SHPLASSSYMFSVGTPPSLVAGHACPRLPGFALL YQHVSVIMAGRSCLHWLTHFLHLSLCKGLDRAS HQWMNTQGGPCPG*CHRDSNQGIWTVPIPKSCL RNPLLQMNSLC*LHLSH*YFARLETCKTTV*QRK MILARKSFCEDGTS*HSPRPYY*KMHFHFDSDF PTREPGWAALSPLASIFPIS*H*LPPVLPSPPS PLQGVPGAAIKTL*AV*RF*QTLTYMESQYKEQM NQSSSIKCFIN*NVLEILFLKPTSTFCRNLFQY LLNNFECVGPSKIYLIPQYCGTPPGASWWSSRF PLEAVSPSRSMTS*VWGVLWGVGQWPGLGLSQFF PFQECTNTSPER*WVAMPSASWAGEWRMAHPTG WLPTPGTLTGVTMVSGCPLPAKNSEL*ATPRGH LGFLCSTHRTALNPGPTARL*APPKCQAVRSSLL L*GQTLSSFRANACNSSHSIAPDPVMGRVTGQG ACPWRCAPRLQKPCGVEDCASGPTAGVARDE*L KVF*G*V*LYWLTLVTL*KHLTFYSQASLKYSED RITVESNQKWWLEFHAPISTGKRSNLPWACRAS PGGEIGVEMHFIL*VHVRYKFQTGSEGLDWPNI RPVFQGDQVLATSQPVVTVQTGHVSHRCQHRASF PL*TSAVGSTCCPS*LWPPP*SIHLQGARQRRR NGKRSS*QDESSPISSPSTSKQVAFHICHRNQR RDGVGSPLENASLPGPLHLSSLQCHNLSDLVLSM IL**KFYFFVHSANHVGEPVEQRETCASFTDCL LMTRLKRKPSGQELFLTH*SLLPQGK*FRRNQL LLFLKNYSFTLSS*QGMPVPIKVFSNLKSTLMGS QILCHCL*TCSCCLSLSLQRITSWNCMFLRLLG LHLNFSLPQPCFQPWHPSPN*FPVILVNLLCKC LVSLPLLKNSKHSCALFFCCCCD*QSEIPSPSAL QPR*QRKAPSQ*LKKKKKKKRPGAVAQACNPST LGG*GGQIT*GQEF*DQPWQHGETPSLLKIQN* PSMLAHACNPSYSGG*GTRIA*TWEAEDAVSRDH AIALQPGGQE*ICVSPKKKRKRKMLNKGYHKPQ IHNHLSFQFQVEYIHNLNKVLPAPKLS*FIPCA LSPSPPSYTHLQLLACF*HGASGDVLVSDKELWG MSTSA*GFVRNTCLAVFVTGKELSVLIHLRSPH *S*LCHLSSIVSPGFLSK*DDRWT*QCLFSLYL ASPENHSRIEIRV*VGSPTSAF

Frame 2

PPVFSIVLLQGVSAADVTDKVWVPTMWADSILS NLREI*EKPDEAKYGGSRL*SQHFGMPRQADHL RSGVRDQSDQHDETPSLLKIKNFARRGGGRL*S QLLGRLRLENRMNPGGRGCGELR*CHFPREDGVV KLHDTAFCTMGMI*EMMRGSWAEVEAGEQGKKH YLD*INLFKNEFIICG*IHLKKSIT*A*KEAYN IASQARCGGSCL*SQHFGRPRQADHLKPGV*DQP GQHGETLSLLKIQKLAGCGGACL*SQLLRRLRH ENRLNSGGRGCSGQRSRHCTPAWMTQ*NSVHSH PPVPPKKKIKRCIEQQNFLLFYEITKDTCLQPSS ACLVLISLLGECGPAKVAFAGIGEGVVGSELVM KTFLYPELCLPRT*SCLQASMHGNNGHSVPPSK RSETRAPVAPAG*GPAGWRGSAGEKVGATLESLG GFGVGTTLTRQVIETF*VPVSISTKITIPLAMN DGED*VEWRAEHPADCKSTCTGGEAAPFP*HAG PSANTRSSHCYSASRKHPFSVSELS*FCHLIVVK YT*QKCNNLSNLLLFSFSFFFFFFFFWRWSLAL SPRLECSGGILAHCNLCLLGSSNSPASVSPVAK TTGMCHHAQLIFVFLVEMGFCHVGQPGLKRLTC* SAFLGLAKCWDYRREPPCPATIFKCTVL*H*VH SQCCSATTTICPPNSFSARKTETLSPLTPIL*P LVSPTLLSVSMNLTPRDLIQVDHNSIYFLGELCF LLRKKHSQVR*LTPVISTLWEAEAGGSPEVRSL RPTWPTW*NPVSTKKTKLSWAWWQAPIIPATIE AEAGELLEPRRRRLQ*AEIAPLHSSLGNKSETPP PGKKKKTTHTHTN*YYTFCRFQK*TQFPGRGSR GCFSCFKS*VGQIVEAGEWGKWLITPLIPALWE AKVGKSLEPGSSRPVWPT*QDPISTKENKS*V*A PGASPGVERSVAYKV*GAPVAL*GDCCRGPSPD MAASPGGARSCRPSGLWKPSLTGSASTPMRTSA WRCRRRTCSHAVAACVGTGESGCASTAGLVLRDP GPQLLSGGDFEDFLGLLRVRSVSGRLRESGRQG LARLLLPGRCLNQGWPGRAV*GVASQQNSQFQK MWWNVWPLVWFVSSHVPFSCLGNQFGAFPYV*LA GMAPP*QQGGGTELSAPAACSLALVPQ*RCSLA AADPKCLRA*KNAWASPLTS*MGITGDSHFREL VVFLSTLGKFSVCSVENQCSTRKGTRSLVWYGHE QESLLSSPGSLWAGVGLAVIPCKLCLSRERPLE GLGSGRSARTTCCSWGWVHEWPRALG*HTVVWR AGGASLRGFH*RLSFQL*WWLSC*SLELLDKKRP GFWWPL*IPCR*VCPLGHFLARWIV*AINHHGF ICLYKLGVETEGLLRGASQVHRPLAGPAWRPCC RHEACLAPGLLRGGCRLTPLSPASHQGADRTPSL PVSTTSTAPGPHARGREIPPSVARSVSLATARP TNRTSTTVRGLGPGHGARGGWGAAASLLTLQRR GQG*GALGSRNSRIEEGE*CPLARRSSTLSTFCL FHPELSLSPSKLEGTKALLLQGSGVSWSLEVSW PLPGPRAHCSVLPFLSFQPQPCP*FFRGAFLEL PWQGKSP*MQSSPGGHQKSVTG*PWRATRHGHCP PQPRARFIFQDTIPTASPIARRTSWPRSTKTAP WRELSLCIRTSCSTSQVRADG*WQ*RVGVGRGA WVPGWFMLTNRAGLGRQVRGWGRGLCGAFHIGSL LCGPAWHLTVFVQVLCQHTQFQRPLPEPLG*FL PIH*RPAHTCSLWERPPPWWLATPALAFLALHC FTSMFLL*WQAVRVCTG*HIFFIFLFAKA*IVPH TSG*THREDLALGSAIGTPTKESGQSPSPSPVL EIPCSR*TVFADCIYPINILLDWKLVKQQFDKG R*FWQEKVSVKMALLSIVLALIIKRCISTLTVIS PPESLVGQP*AP*PVSFPSASISCHQSSLLLPH LFRVYLEQLLKLFKPCNAFDKPLLTWNPSIKNR *ISHLALNVLSIRMY*KFYF*SQQAHFVGIYFNI YSTTLSVLGLQKYI*FPSIVGPPLEPPGGAAGS LWKLFLLPGA*PAECGGCCGAWDSGPVSVSASS SLFRSVPTRHRRDDGWPCHPHPGLGSGEWHTLLA GCQLLEH*LG*QW*VAAPFLPRTVNCEPHPVAI SAFSVLPTAQP*TLDLRPGCELLLSARQSGVPF CCEGRL*AASEPTPATVPTASRQIL*WEG*PGRG LARGGVPHGSRSLVVLRTVLVGQQQEWPGMSDL RSFKDESDYIG*PLSHFKSTLLFIPRLL*NTQR TGSLWNRIRSGGWNSTHRSVLGKDLICRGPVVPV LGARSG*KCILFFKFT*DTSFRQGLKDWIGQTS DLSSKETKSWLHPSLWLQCRQAM*ATAASTERP SPCRLVP*GVPAAPADCGPLRDPSISREQDRDAG MESGVPNRMKVPPSVPPVPPSK*LSTFVTEIRG ETVLGALWRTPVSQAPCIYRVCNVTTSLILCSA *FFNRSFIFSCTLLIMWVSQWNSGRPVLVLQIAS L*RGSKGNQVVRSCF*PTDLYYHKENSLGETSF YCF*KITASPCQVNKECLCQ*KFSPT*SLL*WD LRSFVTAYRLVAAVSLCPCRESRPGTACSCDSWD FILTSRCPSHVFNHGIPPPISSLSSSSTFSVSA W*ACPCLRTQNIAVLYFFVVVVTDRVRFRLPVH SSLGDSARLRLNN*KKKKKKKGRAQWLKPVIPAL WEAEAGRSPEVRSFEISLGNTVKPHLY*KYKIS QACWHMPVIPATREAEVRESLEPGRQRMQ*AEI TPLHSSLGDKSESVSHQKKKEKERCLTKVTISHK FITTYPSSFK*NIFITSIKFSLLPN*VDSFHAH CLPHLHRIRTCSSLPASSMEPAGMCW*VTRNCG ACRHQLKALLETPALQCL*LGKSSVF*YTSDHHI DHNFVTSVQ*FLLDSCPSEMTGGRNNAFSVFT* PLQRITPE*KLEYR*AVQPLH

Sequence CTSB.shed1.aAug05

CACCCCCAGTGTTTTCTATTGTGCTTCTCCAAGGTGTCTCAGCCGCAGATGTGACAGACAAGGTCTGGGTCCCCACTATGTGGGCAGACTCAATCTTGAG TAATTTAAGGGAAATCTAAGAAAAGCCAGATGAGGCCAAGTATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGATGCCAAGGCAAGCGGATCACCTG AGGTCAGGAGTTCGAGACCAGTCTGACCAACATGATGAAACCCCGTCTCTACTAAAAATAAAAAACTTTGCCAGGCGTGGTGGCGGGCGCCTGTAATCCC AGCTACTCGGGAGGCTGAGGCTAGAGAATCGCATGAACCCGGGAGGCAGAGGTTGCGGTGAGCTGAGATGATGCCACTTCCCACGTGAGGATGGGGTAGT TAAGTTGCACGACACTGCCTTCTGCACAATGGGGATGATATGAGAAATGATGAGAGGATCCTGGGCAGAGGTTGAGGCAGGAGAGCAGGGCAAGAAACAC TATCTGGACTGAATAAACTTATTTAAAAATGAGTTTATAATTTGTGGATAAATTCATCTTAAAAAATCCATTACATAAGCCTAGAAAGAAGCGTACAATA TCGCAAGTCAGGCCAGGTGTGGTGGATCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCATTTGAAGCCAGGAGTTTAAGACCAGCC TGGCCAACATGGTGAAACCCTGTCTTTACTAAAAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAC GAGAATCGATTGAACTCAGGAGGCAGAGGTTGCAGTGGGCAGAGATCTCGCCACTGCACTCCAGCCTGGATGACACAGTGAAATTCTGTCCACTCCCACC CTCCCGTCCCACCCAAAAAAAAAATCAAAAGGTGCATTGAGCAACAGAATTTCCTTTTGTTTTATGAAATTACCAAGGACACCTGTCTGCAGCCTTCTTC GGCATGTCTTGTGCTCATTTCCTTGTTAGGGGAGTGTGGCCCAGCCAAAGTGGCCTTTGCAGGGATTGGGGAGGGAGTTGTGGGATCAGAGCTTGTAATG AAGACCTTCCTTTATCCAGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGA GATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGG GGGATTCGGGGTGGGGACAACTCTGACAAGGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAAT GATGGCGAGGATTAGGTGGAGTGGCGGGCAGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCC CGTCCGCAAATACCAGGAGCTCTCACTGCTACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAA ATACACGTAACAGAAATGTAATAATCTTAGCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTG TCACCCAGGCTGGAGTGCAGTGGTGGGATATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGA CTACAGGCATGTGCCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTG ATCTGCCTTCCTCGGCCTCGCAAAATGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACAT TCACAGTGTTGCTCAGCCACCACCACCATCTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCC TGGTAAGCCCCACTCTACTTTCTGTCTCTATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTT TTTGTTAAGAAAAAAACACAGCCAGGTACGGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTG AGACCAACCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGG CTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCC TCCAGGAAAAAAAAAAAAAACCACACACACACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGG GGGTGCTTCTCTTGCTTTAAAAGCTGAGTTGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGG CCAAGGTGGGCAAATCACTTGAACCCGGGAGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGC CCCAGGAGCGTCCCCTGGTGTTGAGAGATCAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGAC ATGGCAGCCTCACCCGGTGGGGCTCGTTCCTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGT GGAGGTGTCGGCGGAGGACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCC TGGGCCCCAGCTTCTCAGTGGAGGGGACTTTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGC CTGGCAAGGCTGCTGCTTCCTGGGAGGTGCCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAA TGTGGTGGAATGTCTGGCCCCTGGTCTGGTTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGC GGGGATGGCTCCACCTTAACAACAAGGTGGTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCG GCTGCAGATCCGAAATGCCTGAGGGCTTAAAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGG TGGTGTTTTTAAGTACCTTAGGGAAGTTTAGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGGTATGGACATGA ACAGGAGAGCCTCCTGTCTTCTCCCGGATCTTTGTGGGCAGGGGTGGGGCTGGCGGTTATTCCCTGCAAGCTGTGCTTATCTAGGGAGCGTCCCTTGGAG GGTTTGGGGTCTGGGAGGTCTGCTCGCACCACTTGCTGCAGCTGGGGGTGGGTCCACGAGTGGCCTCGGGCACTTGGGTAGCACACAGTGGTCTGGAGAG CTGGTGGTGCTTCTCTCAGAGGTTTTCATTAGAGGCTGTCTTTTCAGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCC TGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGTAAGTGTGTCCCCTTGGCCACTTTCTGGCCAGATGGATTGTTTGAGCAATTAACCATCATGGCTTT ATTTGCCTTTATAAACTGGGGGTTGAGACAGAGGGGCTGCTGAGAGGTGCTAGCCAGGTGCACAGGCCTCTGGCAGGACCTGCCTGGCGTCCATGCTGCA GGCACGAGGCTTGCCTTGCCCCAGGTCTTCTCCGTGGGGGCTGTAGGTTGACTCCGCTTTCTCCCGCGTCCCATCAGGGTGCAGACCGTACTCCATCCCT CCCTGTGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCT ACAAACAGGACAAGCACTACGGTAAGGGGCCTGGGGCCTGGCCACGGCGCACGTGGAGGCTGGGGAGCTGCTGCATCCCTCCTCACGCTGCAGCGAAGAG GTCAGGGCTGAGGAGCCTTGGGGTCCCGGAACTCTAGGATAGAGGAGGGGGAGTGATGCCCTCTTGCCAGGAGAAGCAGCACACTCTCCACTTTCTGCCT GTTCCACCCTGAACTCAGCCTCAGCCCTTCCAAACTGGAAGGGACCAAAGCCCTCCTTTTACAAGGGAGTGGCGTGTCCTGGAGTCTTGAGGTATCCTGG CCTCTTCCGGGGCCTCGTGCTCATTGCTCCGTTCTCCCATTTCTGAGCTTCCAGCCCCAGCCCTGTCCTTAGTTCTTCAGGGGAGCCTTCCTTGAGCTCC CCTGGCAGGGCAAGTCCCCTTAGATGCAGTCTTCCCCTGGAGGCCACCAGAAATCAGTGACTGGCTGACCGTGGCGTGCCACGAGGCACGGTCACTGCCC TCCCCAACCCCGAGCTCGGTTCATTTTCCAGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCG TGGAGGGAGCTTTCTCTGTGTATTCGGACTTCCTGCTCTACAAGTCAGGTGCGTGCTGATGGCTGATGGCAATAGAGGGTGGGGGTCGGGAGGGGAGCCT GGGTGCCAGGATGGTTCATGTTGACCAATAGGGCTGGATTGGGCAGGCAGGTGAGGGGCTGGGGAAGAGGGCTGTGTGGGGCCTTCCATATAGGCTCACT CCTCTGTGGACCAGCCTGGCACCTGACTGTCTTTGTCCAGGTGTTGTGCCAACACACACAATTCCAGAGGCCTCTCCCAGAGCCTCTGGGCTAGTTCCTT CCCATCCATTAGCGTCCAGCTCATACATGTTCTCTGTGGGAACGCCCCCCTCCCTGGTGGCTGGCCACGCCTGCCCTCGCCTTCCTGGCTTTGCATTGCT TTACCAGCATGTTTCTGTTATAATGGCAGGCCGTTCGTGTTTGCACTGGCTGACACATTTTCTTCATCTTTCTCTTTGCAAAGGCCTAGATCGTGCCTCA CACCAGTGGATGAACACACAGGGAGGACCTTGCCCTGGGTAGTGCCATAGGGACTCCAACCAAGGAATCTGGACAGTCCCCATCCCCAAGTCCTGTCTTA GAAATCCCTTGCTCCAGATGAACAGTCTTTGCTGACTGCATCTATCCCATTAATATTTTGCTAGATTGGAAACTTGTAAAACAACAGTTTGACAAAGGAA GATGATTCTGGCAAGAAAAAGTTTCTGTGAAGATGGCACTTCTTAGCATAGTCCTCGCCCTTATTATTAAAAGATGCATTTCCACTTTGACAGTGATTTC CCCACCCGAGAGCCTGGTTGGGCAGCCCTGAGCCCCTTAGCCAGTATCTTTCCCATCAGCTAGCATTAGCTGCCACCAGTCCTCCCTTCTCCTCCCTCAC CTCTTCAGGGTGTACCTGGAGCAGCTATTAAAACTCTTTAAGCCGTGTAACGCTTTTGACAAACCCTTACTTACATGGAATCCCAGTATAAAGAACAGAT GAATCAGTCATCTAGCATTAAATGTTTTATCAATTAGAATGTATTAGAAATTCTATTTTTAAAGCCAACAAGCACATTTTGTAGGAATCTATTTCAATAT TTACTCAACAACTTTGAGTGTGTTGGGCCTTCAAAAATATATTTAATTCCCCAGTATTGTGGGACCCCCCCTGGAGCCTCCTGGTGGAGCAGCAGGTTCC CTCTGGAAGCTGTTTCTCCTTCCCGGAGCATGACCAGCTGAGTGTGGGGGGTGCTGTGGGGCGTGGGACAGTGGCCCGGTCTCGGTCTCAGCCAGTTCTT CCCTTTTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGC TGGTTGCCAACTCCTGGAACACTGACTGGGGTGACAATGGTGAGTGGCTGCCCCCTTCCTGCCAAGAACAGTGAATTGTGAGCCACACCCCGTGGCCATC TCGGCTTTCTCTGTTCTACCCACCGCACAGCCTTAAACCCTGGACCTACGGCCAGGCTGTGAGCTCCTCCTAAGTGCCAGGCAGTCAGGAGTTCCCTTTT GCTGTGAGGGCAGACTCTGAGCAGCTTCAGAGCCAACGCCTGCAACAGTTCCCACAGCATCGCGCCAGATCCTGTGATGGGAAGGGTGACCGGGCAGGGG GCTTGCCCGTGGAGGTGTGCCCCACGGCTCCAGAAGCCTTGTGGTGTTGAGGACTGTGCTAGTGGGCCAACAGCAGGAGTGGCCAGGGATGAGTGACTTA AGGTCTTTTAAGGATGAGTCTGACTATATTGGTTGACCCTTGTCACACTTTAAAAGCACCTTACTTTTTATTCCCAGGCTTCTTTAAAATACTCAGAGGA CAGGATCACTGTGGAATCGAATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGT CCTGGGGGCGAGATCGGGGTAGAAATGCATTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCA GACCTGTCTTCCAAGGAGACCAAGTCCTGGCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTT CCCCCTGTAGACTAGTGCCGTAGGGAGTACCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGG AATGGAAAGCGGAGTTCCTAACAGGATGAAAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGA GAGACGGTGTTGGGAGCCCTTTGGAGAACGCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCAT GATTCTTTAATAGAAGTTTTATTTTTTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTC CTTATGACGCGGCTCAAAAGGAAACCAAGTGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTT TACTGTTTTTGAAAAATTACAGCTTCACCCTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATC TCAGATCCTTTGTCACTGCCTATAGACTTGTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGA CTTCATCTTAACTTCTCGCTGCCCCAGCCATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCC TGGTAAGCTTGCCCTTGCTTAAGAACTCAAAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGA GTGCAGTGGCGCCTTCTCAGCTCACTGCAACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCA CCAATATGCCTGGGTAATTTTTGTATTTTTAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGC CTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTC ATAAAACAGGCTTGGTGTCTTGGTGATCTCACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGG AGTCAGGGCCTGTATACAGTATTTTGAATTTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGT TATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTA TGAGGCTAGCTCTTTCCAAAAGCATTCAAGTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGA ACATTGTAGGAAAGGTTTGTTAAGCATCTCTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC CAAGGTGGGAGGATGACAAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTG GCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCAC TCCAGCCTCGGTGACAGCGCAAGGCTCCGTCTCAATAATT

CTSB.F10 (forward primer)
CTSB.R7 (reverse primer)
Intron retention

Frame 0

HPQCFLLCFSKVSQPQM*QTRSGSPLCGQTQS* VI*GKSKKSQMRPSMVAHACNPSTLGCQGKRIT* GQEFETSLTNMMKPRLY*K*KTLPGVVAGACNP SYSGG*G*RIA*TREAEVAVS*DDATSHVRMG* LSCTTLPSAQWG*YEK**EDPGQRLRQESRARNT IWTE*TYLKMSL*FVDKFILKNPLHKPRKKRTI SQVRPGVVDHACNPSTLGGQGRQII*SQEFKTS LANMVKPCLY*KYKN*PGVVVHACNPSYSGG*GT RID*TQEAEVAVGRDLATALQPG*HSEILSTPT LPSHPKKKSKGALSNRISFCFMKLPRTPVCSLL RHVLCSFPC*GSVAQPKWPLQGLGRELWDQSL** RPSFIQSYVYRGPEAACKLRCTGTMATVSHHQR DQRPGLLWLLLGKALLAGGEALERKWEQHWRVL GDSGWGQL*QGKL*KLSESQFPSVQKSQSLWP*M MARIRWSGGQSIQQIASPRVQVAKQLPFPDMLA RPQIPGALTATLLQESIPLVSVSCLNFVI*LW* NTRNRNVIILAIFFCFLFLSFFFFFFFGDGVLLC HPGWSAVVGYWLTATSASWVQAILLPQSPQ*LR LQACATTPS*FLYF**RWGFAMLDSLVSNASPV DLPSSASQNAGITGVSHRAQQLFSSVQFCSIKYI HSVAQPPPPSVPRTLFQLARQKLCPH*HQYCSP W*APLYFLSL*I*LLGTSYKWITTVFIFWVSCV FC*EKNTARYGSSRL*SQHFGRLRQADHLRSGV* DQPGQHGETLSLLKRQNLAGPGGRHL*SQLL*R LRQENCLNPGGVGCSELRLRHCIPAWATKVKLR LQEKKKKPHTHIQTNTTHFADFRNEPSFQAEVHG GASLALKAELGKSLRQESGASGSSRL*SQHFGR PRWANHLNPGVQDQSGQRSKTPFLPKKIKAEFE PQERPLVLRDQLPTRSEVPLWPFEGTAAEGPART WQPHPVGLVPAGLRGCGSHL*PDLHPHQCARQR GGVGGGPAHMLWQHVWGRVSQAVLPQRV*C*ET LGPSFSVEGTLRTSWDCCESEVFRGDSESLAGRA WQGCCFLGGA*TKAGPAELSEGWHPSKTVSFRK CGGMSGPWSGLCHRMFLFPAWGTSLGHFLMCS* RGWLHLNNKVVAQNFLLQLPAASPWFRSSGVHWR LQIRNA*GLKKMHGLLP*LVKWESLAILISENW WCF*VP*GSLASAQLRISVLPGRALVAWSGMDM NRRASCLLPDLCGQGWGWRLFPASCAYLGSVPWR VWGLGGLLAPLAAAGGGSTSGLGHLGSTQWSGE LVVLLSEVFIRGCLFSCNGGYPAEAWNFWTRKG LVSGGLYESHVGKCVPLATFWPDGLFEQLTIMAL FAFINWGLRQRGC*EVLARCTGLWQDLPGVHAA GTRLALPQVFSVGAVG*LRFLPRPIRVQTVLHP SL*APRQRLPAPMHGGGRYPQV*QDL*AWLQPDL QTGQALR*GAWGLATAHVEAGELLHPSSRCSEE VRAEEPWGPGTLG*RRGSDALLPGEAAHSPLSA CSTLNSASALPNWKGPKPSFYKGVACPGVLRYPG LFRGLVLIAPFSHF*ASSPSPVLSSSGEPSLSS PGRASPLRCSLPLEATRNQ*LADRGVPRGTVTA LPNPELGSFSRIQFLQRLQ*REGHHGRDLQKRPR GGSFLCVFGLPALQVRCVLMADGNRGWGSGGEP GCQDGSC*PIGLDWAGR*GAGEEGCVGPSI*AH SSVDQPGT*LSLSRCCANTHNSRGLSQSLWASSF PSISVQLIHVLCGNAPLPGGWPRLPSPSWLCIA LPACFCYNGRPFVFALADTFSSSFSLQRPRSCL TPVDEHTGRTLPWVVP*GLQPRNLDSPHPQVLS* KSLAPDEQSLLTASIPLIFC*IGNL*NNSLTKE DDSGKKKFL*RWHFLA*SSPLLLKDAFPL*Q*F PHPRAWLGSPEPLSQYLSHQLALAATSPPFSSLT SSGCTWSSY*NSLSRVTLLTNPYLHGIPV*RTD ESVI*H*MFYQLECIRNSIFKANKHIL*ESISI FTQQL*VCWAFKNIFNSPVLWDPPWSLLVEQQVP SGSCFSFPEHDQLSVGGAVGRGTVARSRSQPVL PFSGVYQHVTGEMMGGHAIRILGWGVENGTPYW LVANSWNTDWGDNGEWLPPSCQEQ*IVSHTPWPS RLSLFYPPHSLKPWTYGQAVSSS*VPGSQEFPF AVRADSEQLQSQRLQQFPQHRARSCDGKGDRAG GLPVEVCPTAPEALWC*GLC*WANSRSGQG*VT* GLLRMSLTILVDPCHTLKAPYFLFPGFFKILRG QDHCGIESEVVAGIPRTDQYWEKI*SAVGLSCQ SWGRDRGRNAFYSLSSRKIQVSDRV*RTGLAKHQ TCLPRRPSPGYIPACGYSADRPCEPPLPAQSVL PPVD*CRREYLLPQLTVAPSVIHPSPGSKTETQ EWKAEFLTG*KFPHQFPQYLQASSFPHLSQKSEE RRCWEPFGERQSPRPPASIEFAMSQPL*SCAQH DSLIEVLFFRALC*SCG*ASGTAGDLC*FYRLP PYDAAQKETKWSGVVSDPLISTTTRKIV*EKPAF TVFEKLQLHPVKLTRNACANKSFLQLEVYSDGI SDPLSLPIDL*LLSLFVPAENHVLELHVLATLG TSS*LLAAPAMFSTMASLPQLVPCHPRQPSL*VP GKLALA*ELKT*LCSIFLLLL*LTE*DSVSQAG VQWRLLSSLQPAAS*IQAILLLQPSE*LG*QAL TNMPG*FLYF*VHTGFHHVGQASFKLPASGGLPA SASQSVGITGVSHWALPVFFISHKSSNKLRIQL IKQAWCLGDLT*PRCYPVGNHIPLDALQPWFGL ESGPVYSILNLYATGLHCWSGTLVLCIALV*KHV IAVLGIEQTRRTSNHSTVLPRYTSVLPFPTEVV *G*LFPKAFKFGF*CDSEFRNQMLDQISSENLR NIVGKVC*ASLKCHDEHNSRPSWLTPVIPALWEA KVGG*QGQEFKTSLANMLKPHLY*KYKN*LGMV AHACNPSYLGG*GRRIA*TREAEVAVSQDSASA LQPR*QRKAPSQ*

Frame 1

TPSVFYCASPRCLSRRCDRQGLGPHYVGRLNLE *FKGNLRKAR*GQVWWLTPVIPALWDAKASGSP EVRSSRPV*PT**NPVSTKNKKLCQAWWRAPVIP ATREAEARESHEPGRQRLR*AEMMPLPT*GWGS *VARHCLLHNGDDMRNDERILGRG*GRRAGQET LSGLNKLI*K*VYNLWINSS*KIHYISLERSVQY RKSGQVWWIMPVIPALWEAKAGRSFEARSLRPA WPTW*NPVFTKNTKISRVWWCMPVIPATQEAEA RESIELRRQRLQWAEISPLHSSLDDTVKFCPLPP SRPTQKKNQKVH*ATEFPFVL*NYQGHLSAAFF GMSCAHFLVRGVWPSQSGLCRDWGGSCGIRACN EDLPLSRVMFTEDLKLPASFDAREQWPQCPTIKE IRDQGSCGSCWVRPCWLAGKRWRESGSNTGESW GIRGGDNSDKASYRNFLSPSFHQYKNHNPSGHE *WRGLGGVAGRASSRLQVHVYRWRSSSLSLTCWP VRKYQELSLLLCFKKASL*CQ*AVLILSFNCGK IHVTEM**S*QSSFVFFFFLFFFFFFLEMESCS VTQAGVQWWDIGSLQPLPPGFKQFSCLSLPSS*D YRHVPPRPANFCIFSRDGVLPCWTAWSQTPHLL ICLPRPRKMLGLQA*ATVPSNYFQVYSSVALST FTVLLSHHHHLSPELFFSSQDRNSVPINTNIVAP GKPHSTFCLYEFDS*GPHTSGSQQYLFSG*AVF FVKKKTQPGTVAHACNLNTLGG*GRRIT*GQEF ETNLANMVKPCLY*KDKT*LGLVAGTYNPSYYRG *GRRIA*TQEA*VAVS*DCATAFQPGQQK*NSA SRKKKKNHTHTYKLILHILQISEMNPVSRQRFT GVLLLL*KLSWANR*GRRVGQVAHHASNPSTLGG QGGQIT*TREFKTSLANVARPHFYQRK*KLSLS PRSVPWC*EISCLQGLRCPCGPLRGLLQRAQPG HGSLTRWGSFLQAFGAVEAISDRICIHTNAHVSV EVSAEDLLTCCGSMCGDG*VRLCFHSGFSAERP WAPASQWRGL*GLPGTAASQKCFGETPRVWQAG PGKAAASWEVPEPRLARQSCLRGGIPAKQSVSEN VVECLAPGLVCVIACSFFLLGEPVWGISLCVVS GDGSTLTTRWWHRTFCSSCLQPRLGSAVAVFTG GCRSEMPEGLKKCMGFSPN*LNGNHWRFSFQRTG GVFKYLREV*RLLS*ESVFYPEGHS*PGLVWT* TGEPPVFSRIFVGRGGAGGYSLQAVLI*GASLG GFGVWEVCSHHLLQLGVGPRVASGTWVAHSGLES WWCFSQRFSLEAVFSAVMVAILLKLGTSGQEKA WFLVASMNPM*VSVSPWPLSGQMDCLSN*PSWL YLPL*TGG*DRGAAERC*PGAQASGRTCLASMLQ ARGLPCPRSSPWGL*VDSAFSRVPSGCRPYSIP PCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPT YKQDKHYGKGPGAWPRRTWRLGSCCIPPHAAAKR SGLRSLGVPEL*DRGGGVMPSCQEKQHTLHFLP VPP*TQPQPFQTGRDQSPPFTREWRVLES*GIL ASSGASCSLLRSPISELPAPALSLVLQGSLP*AP LAGQVPLDAVFPWRPPEISDWLTVACHEARSLP SPTPSSVHFPGYNSYSVSNSEKDIMAEIYKNGP VEGAFSVYSDFLLYKSGAC*WLMAIEGGGREGSL GARMVHVDQ*GWIGQAGEGLGKRAVWGLPYRLT PLWTSLAPDCLCPGVVPTHTIPEASPRASGLVP SHPLASSSYMFSVGTPPSLVAGHACPRLPGFALL YQHVSVIMAGRSCLHWLTHFLHLSLCKGLDRAS HQWMNTQGGPCPG*CHRDSNQGIWTVPIPKSCL RNPLLQMNSLC*LHLSH*YFARLETCKTTV*QRK MILARKSFCEDGTS*HSPRPYY*KMHFHFDSDF PTREPGWAALSPLASIFPIS*H*LPPVLPSPPS PLQGVPGAAIKTL*AV*RF*QTLTYMESQYKEQM NQSSSIKCFIN*NVLEILFLKPTSTFCRNLFQY LLNNFECVGPSKIYLIPQYCGTPPGASWWSSRF PLEAVSPSRSMTS*VWGVLWGVGQWPGLGLSQFF PFQECTNTSPER*WVAMPSASWAGEWRMAHPTG WLPTPGTLTGVTMVSGCPLPAKNSEL*ATPRGH LGFLCSTHRTALNPGPTARL*APPKCQAVRSSLL L*GQTLSSFRANACNSSHSIAPDPVMGRVTGQG ACPWRCAPRLQKPCGVEDCASGPTAGVARDE*L KVF*G*V*LYWLTLVTL*KHLTFYSQASLKYSED RITVESNQKWWLEFHAPISTGKRSNLPWACRAS PGGEIGVEMHFIL*VHVRYKFQTGSEGLDWPNI RPVFQGDQVLATSQPVVTVQTGHVSHRCQHRASF PL*TSAVGSTCCPS*LWPPP*SIHLQGARQRRR NGKRSS*QDESSPISSPSTSKQVAFHICHRNQR RDGVGSPLENASLPGPLHLSSLQCHNLSDLVLSM IL**KFYFFVHSANHVGEPVEQRETCASFTDCL LMTRLKRKPSGQELFLTH*SLLPQGK*FRRNQL LLFLKNYSFTLSS*QGMPVPIKVFSNLKSTLMGS QILCHCL*TCSCCLSLSLQRITSWNCMFLRLLG LHLNFSLPQPCFQPWHPSPN*FPVILVNLLCKC LVSLPLLKNSKHSCALFFCCCCD*QSEIPSPRLE CSGAFSAHCNLQPPRFKRFSCFSLPSSWDDRHS PICLGNFCIFKYIQDFTMLARLVSNSRPQVVCL PQPPKVLGLQA*ATGPCLYFLSATNPATS*GFSS *NRLGVLVISHNQDATPWGTTSPWMPSSLGLGW SQGLYTVF*ICMPLVCIAGQEL*CFA*PWFRNM L*QFLV*SKLEEPAIIPLSCQGTPQYSPSQLKWY EASSFQKHSSLASDVTQNLGTRC*IK*ALKI*G TL*ERFVKHLLSAMMSITAGRRGSRL*SQHFGR PRWEDDKVRSSRPAWPTC*NLTSTKNTKISWAWW HMPVIPATWEAEAGESLEPGRRRLQ*AKTVPVH SSLGDSARLRLNN

Frame 2

PPVFSIVLLQGVSAADVTDKVWVPTMWADSILS NLREI*EKPDEAKYGGSRL*SQHFGMPRQADHL RSGVRDQSDQHDETPSLLKIKNFARRGGGRL*S QLLGRLRLENRMNPGGRGCGELR*CHFPREDGVV KLHDTAFCTMGMI*EMMRGSWAEVEAGEQGKKH YLD*INLFKNEFIICG*IHLKKSIT*A*KEAYN IASQARCGGSCL*SQHFGRPRQADHLKPGV*DQP GQHGETLSLLKIQKLAGCGGACL*SQLLRRLRH ENRLNSGGRGCSGQRSRHCTPAWMTQ*NSVHSH PPVPPKKKIKRCIEQQNFLLFYEITKDTCLQPSS ACLVLISLLGECGPAKVAFAGIGEGVVGSELVM KTFLYPELCLPRT*SCLQASMHGNNGHSVPPSK RSETRAPVAPAG*GPAGWRGSAGEKVGATLESLG GFGVGTTLTRQVIETF*VPVSISTKITIPLAMN DGED*VEWRAEHPADCKSTCTGGEAAPFP*HAG PSANTRSSHCYSASRKHPFSVSELS*FCHLIVVK YT*QKCNNLSNLLLFSFSFFFFFFFFWRWSLAL SPRLECSGGILAHCNLCLLGSSNSPASVSPVAK TTGMCHHAQLIFVFLVEMGFCHVGQPGLKRLTC* SAFLGLAKCWDYRREPPCPATIFKCTVL*H*VH SQCCSATTTICPPNSFSARKTETLSPLTPIL*P LVSPTLLSVSMNLTPRDLIQVDHNSIYFLGELCF LLRKKHSQVR*LTPVISTLWEAEAGGSPEVRSL RPTWPTW*NPVSTKKTKLSWAWWQAPIIPATIE AEAGELLEPRRRRLQ*AEIAPLHSSLGNKSETPP PGKKKKTTHTHTN*YYTFCRFQK*TQFPGRGSR GCFSCFKS*VGQIVEAGEWGKWLITPLIPALWE AKVGKSLEPGSSRPVWPT*QDPISTKENKS*V*A PGASPGVERSVAYKV*GAPVAL*GDCCRGPSPD MAASPGGARSCRPSGLWKPSLTGSASTPMRTSA WRCRRRTCSHAVAACVGTGESGCASTAGLVLRDP GPQLLSGGDFEDFLGLLRVRSVSGRLRESGRQG LARLLLPGRCLNQGWPGRAV*GVASQQNSQFQK MWWNVWPLVWFVSSHVPFSCLGNQFGAFPYV*LA GMAPP*QQGGGTELSAPAACSLALVPQ*RCSLA AADPKCLRA*KNAWASPLTS*MGITGDSHFREL VVFLSTLGKFSVCSVENQCSTRKGTRSLVWYGHE QESLLSSPGSLWAGVGLAVIPCKLCLSRERPLE GLGSGRSARTTCCSWGWVHEWPRALG*HTVVWR AGGASLRGFH*RLSFQL*WWLSC*SLELLDKKRP GFWWPL*IPCR*VCPLGHFLARWIV*AINHHGF ICLYKLGVETEGLLRGASQVHRPLAGPAWRPCC RHEACLAPGLLRGGCRLTPLSPASHQGADRTPSL PVSTTSTAPGPHARGREIPPSVARSVSLATARP TNRTSTTVRGLGPGHGARGGWGAAASLLTLQRR GQG*GALGSRNSRIEEGE*CPLARRSSTLSTFCL FHPELSLSPSKLEGTKALLLQGSGVSWSLEVSW PLPGPRAHCSVLPFLSFQPQPCP*FFRGAFLEL PWQGKSP*MQSSPGGHQKSVTG*PWRATRHGHCP PQPRARFIFQDTIPTASPIARRTSWPRSTKTAP WRELSLCIRTSCSTSQVRADG*WQ*RVGVGRGA WVPGWFMLTNRAGLGRQVRGWGRGLCGAFHIGSL LCGPAWHLTVFVQVLCQHTQFQRPLPEPLG*FL PIH*RPAHTCSLWERPPPWWLATPALAFLALHC FTSMFLL*WQAVRVCTG*HIFFIFLFAKA*IVPH TSG*THREDLALGSAIGTPTKESGQSPSPSPVL EIPCSR*TVFADCIYPINILLDWKLVKQQFDKG R*FWQEKVSVKMALLSIVLALIIKRCISTLTVIS PPESLVGQP*AP*PVSFPSASISCHQSSLLLPH LFRVYLEQLLKLFKPCNAFDKPLLTWNPSIKNR *ISHLALNVLSIRMY*KFYF*SQQAHFVGIYFNI YSTTLSVLGLQKYI*FPSIVGPPLEPPGGAAGS LWKLFLLPGA*PAECGGCCGAWDSGPVSVSASS SLFRSVPTRHRRDDGWPCHPHPGLGSGEWHTLLA GCQLLEH*LG*QW*VAAPFLPRTVNCEPHPVAI SAFSVLPTAQP*TLDLRPGCELLLSARQSGVPF CCEGRL*AASEPTPATVPTASRQIL*WEG*PGRG LARGGVPHGSRSLVVLRTVLVGQQQEWPGMSDL RSFKDESDYIG*PLSHFKSTLLFIPRLL*NTQR TGSLWNRIRSGGWNSTHRSVLGKDLICRGPVVPV LGARSG*KCILFFKFT*DTSFRQGLKDWIGQTS DLSSKETKSWLHPSLWLQCRQAM*ATAASTERP SPCRLVP*GVPAAPADCGPLRDPSISREQDRDAG MESGVPNRMKVPPSVPPVPPSK*LSTFVTEIRG ETVLGALWRTPVSQAPCIYRVCNVTTSLILCSA *FFNRSFIFSCTLLIMWVSQWNSGRPVLVLQIAS L*RGSKGNQVVRSCF*PTDLYYHKENSLGETSF YCF*KITASPCQVNKECLCQ*KFSPT*SLL*WD LRSFVTAYRLVAAVSLCPCRESRPGTACSCDSWD FILTSRCPSHVFNHGIPPPISSLSSSSTFSVSA W*ACPCLRTQNIAVLYFFVVVVTDRVRFRLPGW SAVAPSQLTATCSLLDSSDSPASAFRVAGMTGTH QYAWVIFVFLSTYRISPCWPG*FQTPGLRWSAC LSLPKCWDYRREPLGPACIFYQPQIQQQAEDSA HKTGLVSW*SHITKMLPRGEPHPPGCPPALVWAG VRACIQYFEFVCHWFALLVRNSSALHSPGLETC YSSSWYRAN*KNQQSFHCPAKVHLSTPLPN*SG MRLALSKSIQVWLLM*LRI*EPDARSNKL*KSEE HCRKGLLSIS*VP**A*QPAVVAHACNPSTLGG QGGRMTRSGVQDQPGQHAETSPLLKIQKLAGHG GTCL*SQLLGRLRQENRLNPGGGGCSEPRQCQCT PASVTAQGSVSII

Sequence CTSB.qAug05

GGGGCGGGGCCGGGAGGGTACTTAGGGCCGGGGCTGGCCCAGGCTACGGCGGCTGCAGGGCTCCGGCAACCGCTCCGGCAACGCCAACCGCTCCGCTGCG CGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGGTCTTGCTTTGTCTCCCAGGCTGGTGTGCAGTGGTGCGACCACGGCTCACGGCAGCCTCAGC CACCCAGATGTAAGCGATCTGGTTCCCACCTCAGCCTCCCGAGTAGATACTTCTGAAAATAGAAATGATGACTCTGGGATGCAAACGTTGGCTGTCCTAT GTATAAGGAGATGGCTTTTCACGCTCCCAGTGACTGAGGAAGTTTCTCCCAGATGGCGCTGCTCTGAGCCTGGTGCAGGGTGTCTTCAGGCCTATGGAGA GCAGCTTGCGTGGGCTGGGCCTGCAGTACCTGGTTTGCATAGATGATTGGCAGGTGGATCTAGGATCCGGCTTCCAACATGTGGCAGCTCTGGGCCTCCC TCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTG GCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTT ACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCT GCTGGGTAAGGCCCTGCTGGCTGGCGGGGAAGCGCTGGAGAGAAAGTGGGAGCAACACTGGAGAGTCTTGGGGGATTCGGGGTGGGGACAACTCTGACAA GGCAAGTTATAGAAACTTTCTGAGTCCCAGTTTCCATCAGTACAAAAATCACAATCCCTCTGGCCATGAATGATGGCGAGGATTAGGTGGAGTGGCGGGC AGAGCATCCAGCAGATTGCAAGTCCACGTGTACAGGTGGCGAAGCAGCTCCCTTTCCCTGACATGCTGGCCCGTCCGCAAATACCAGGAGCTCTCACTGC TACTCTGCTTCAAGAAAGCATCCCTTTAGTGTCAGTGAGCTGTCTTAATTTTGTCATTTAATTGTGGTAAAATACACGTAACAGAAATGTAATAATCTTA GCAATCTTCTTTTGTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGGGAT ATTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGTCTCCCCAGTAGCTAAGACTACAGGCATGTGCCACCACGCCCAGCTA ATTTTTGTATTTTTAGTAGAGATGGGGTTTTGCCATGTTGGACAGCCTGGTCTCAAACGCCTCACCTGTTGATCTGCCTTCCTCGGCCTCGCAAAATGCT GGGATTACAGGCGTGAGCCACCGTGCCCAGCAACTATTTTCAAGTGTACAGTTCTGTAGCATTAAGTACATTCACAGTGTTGCTCAGCCACCACCACCAT CTGTCCCCCGAACTCTTTTTCAGCTCGCAAGACAGAAACTCTGTCCCCATTAACACCAATATTGTAGCCCCTGGTAAGCCCCACTCTACTTTCTGTCTCT ATGAATTTGACTCCTAGGGACCTCATACAAGTGGATCACAACAGTATTTATTTTCTGGGTGAGCTGTGTTTTTTGTTAAGAAAAAAACACAGCCAGGTAC GGTAGCTCACGCCTGTAATCTCAACACTTTGGGAGGCTGAGGCAGGCGGATCACCTGAGGTCAGGAGTTTGAGACCAACCTGGCCAACATGGTGAAACCC TGTCTCTACTAAAAAGACAAAACTTAGCTGGGCCTGGTGGCAGGCACCTATAATCCCAGCTACTATAGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGG AGGCGTAGGTTGCAGTGAGCTGAGATTGCGCCACTGCATTCCAGCCTGGGCAACAAAAGTGAAACTCCGCCTCCAGGAAAAAAAAAAAAAACCACACACA CACATACAAACTAATACTACACATTTTGCAGATTTCAGAAATGAACCCAGTTTCCAGGCAGAGGTTCACGGGGGTGCTTCTCTTGCTTTAAAAGCTGAGT TGGGCAAATCGTTGAGGCAGGAGAGTGGGGCAAGTGGCTCATCACGCCTCTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAAATCACTTGAACCCGGG AGTTCAAGACCAGTCTGGCCAACGTAGCAAGACCCCATTTCTACCAAAGAAAATAAAAGCTGAGTTTGAGCCCCAGGAGCGTCCCCTGGTGTTGAGAGAT CAGTTGCCTACAAGGTCTGAGGTGCCCCTGTGGCCCTTTGAGGGGACTGCTGCAGAGGGCCCAGCCCGGACATGGCAGCCTCACCCGGTGGGGCTCGTTC CTGCAGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACAT GCTGTGGCAGCATGTGTGGGGACGGGTGAGTCAGGCTGTGCTTCCACAGCGGGTTTAGTGCTGAGAGACCCTGGGCCCCAGCTTCTCAGTGGAGGGGACT TTGAGGACTTCCTGGGACTGCTGCGAGTCAGAAGTGTTTCGGGGAGACTCCGAGAGTCTGGCAGGCAGGGCCTGGCAAGGCTGCTGCTTCCTGGGAGGTG CCTGAACCAAGGCTGGCCCGGCAGAGCTGTCTGAGGGGTGGCATCCCAGCAAAACAGTCAGTTTCAGAAAATGTGGTGGAATGTCTGGCCCCTGGTCTGG TTTGTGTCATCGCATGTTCCTTTTTCCTGCTTGGGGAACCAGTTTGGGGCATTTCCTTATGTGTAGTTAGCGGGGATGGCTCCACCTTAACAACAAGGTG GTGGCACAGAACTTTCTGCTCCAGCTGCCTGCAGCCTCGCCTTGGTTCCGCAGTAGCGGTGTTCACTGGCGGCTGCAGATCCGAAATGCCTGAGGGCTTA AAAAAATGCATGGGCTTCTCCCCTAACTAGTTAAATGGGAATCACTGGCGATTCTCATTTCAGAGAACTGGTGGTGTTTTTAAGTACCTTAGGGAAGTTT AGCGTCTGCTCAGTTGAGAATCAGTGTTCTACCCGGAAGGGCACTCGTAGCCTGGTCTGGTATGGACATGAACAGGAGAGCCTCCTGTCTTCTCCCGGAT CTTTGTGGGCAGGGGTGGGGCTGGCGGTTATTCCCTGCAAGCTGTGCTTATCTAGGGAGCGTCCCTTGGAGGGTTTGGGGTCTGGGAGGTCTGCTCGCAC CACTTGCTGCAGCTGGGGGTGGGTCCACGAGTGGCCTCGGGCACTTGGGTAGCACACAGTGGTCTGGAGAGCTGGTGGTGCTTCTCTCAGAGGTTTTCAT TAGAGGCTGTCTTTTCAGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCAT GTAGGTAAGTGTGTCCCCTTGGCCACTTTCTGGCCAGATGGATTGTTTGAGCAATTAACCATCATGGCTTTATTTGCCTTTATAAACTGGGGGTTGAGAC AGAGGGGCTGCTGAGAGGTGCTAGCCAGGTGCACAGGCCTCTGGCAGGACCTGCCTGGCGTCCATGCTGCAGGCACGAGGCTTGCCTTGCCCCAGGTCTT CTCCGTGGGGGCTGTAGGTTGACTCCGCTTTCTCCCGCGTCCCATCAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCG GCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACGGTAAGGGG CCTGGGGCCTGGCCACGGCGCACGTGGAGGCTGGGGAGCTGCTGCATCCCTCCTCACGCTGCAGCGAAGAGGTCAGGGCTGAGGAGCCTTGGGGTCCCGG AACTCTAGGATAGAGGAGGGGGAGTGATGCCCTCTTGCCAGGAGAAGCAGCACACTCTCCACTTTCTGCCTGTTCCACCCTGAACTCAGCCTCAGCCCTT CCAAACTGGAAGGGACCAAAGCCCTCCTTTTACAAGGGAGTGGCGTGTCCTGGAGTCTTGAGGTATCCTGGCCTCTTCCGGGGCCTCGTGCTCATTGCTC CGTTCTCCCATTTCTGAGCTTCCAGCCCCAGCCCTGTCCTTAGTTCTTCAGGGGAGCCTTCCTTGAGCTCCCCTGGCAGGGCAAGTCCCCTTAGATGCAG TCTTCCCCTGGAGGCCACCAGAAATCAGTGACTGGCTGACCGTGGCGTGCCACGAGGCACGGTCACTGCCCTCCCCAACCCCGAGCTCGGTTCATTTTCC AGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGAC TTCCTGCTCTACAAGTCAGGTGCGTGCTGATGGCTGATGGCAATAGAGGGTGGGGGTCGGGAGGGGAGCCTGGGTGCCAGGATGGTTCATGTTGACCAAT AGGGCTGGATTGGGCAGGCAGGTGAGGGGCTGGGGAAGAGGGCTGTGTGGGGCCTTCCATATAGGCTCACTCCTCTGTGGACCAGCCTGGCACCTGACTG TCTTTGTCCAGGTGTTGTGCCAACACACACAATTCCAGAGGCCTCTCCCAGAGCCTCTGGGCTAGTTCCTTCCCATCCATTAGCGTCCAGCTCATACATG TTCTCTGTGGGAACGCCCCCCTCCCTGGTGGCTGGCCACGCCTGCCCTCGCCTTCCTGGCTTTGCATTGCTTTACCAGCATGTTTCTGTTATAATGGCAG GCCGTTCGTGTTTGCACTGGCTGACACATTTTCTTCATCTTTCTCTTTGCAAAGGCCTAGATCGTGCCTCACACCAGTGGATGAACACACAGGGAGGACC TTGCCCTGGGTAGTGCCATAGGGACTCCAACCAAGGAATCTGGACAGTCCCCATCCCCAAGTCCTGTCTTAGAAATCCCTTGCTCCAGATGAACAGTCTT TGCTGACTGCATCTATCCCATTAATATTTTGCTAGATTGGAAACTTGTAAAACAACAGTTTGACAAAGGAAGATGATTCTGGCAAGAAAAAGTTTCTGTG AAGATGGCACTTCTTAGCATAGTCCTCGCCCTTATTATTAAAAGATGCATTTCCACTTTGACAGTGATTTCCCCACCCGAGAGCCTGGTTGGGCAGCCCT GAGCCCCTTAGCCAGTATCTTTCCCATCAGCTAGCATTAGCTGCCACCAGTCCTCCCTTCTCCTCCCTCACCTCTTCAGGGTGTACCTGGAGCAGCTATT AAAACTCTTTAAGCCGTGTAACGCTTTTGACAAACCCTTACTTACATGGAATCCCAGTATAAAGAACAGATGAATCAGTCATCTAGCATTAAATGTTTTA TCAATTAGAATGTATTAGAAATTCTATTTTTAAAGCCAACAAGCACATTTTGTAGGAATCTATTTCAATATTTACTCAACAACTTTGAGTGTGTTGGGCC TTCAAAAATATATTTAATTCCCCAGTATTGTGGGACCCCCCCTGGAGCCTCCTGGTGGAGCAGCAGGTTCCCTCTGGAAGCTGTTTCTCCTTCCCGGAGC ATGACCAGCTGAGTGTGGGGGGTGCTGTGGGGCGTGGGACAGTGGCCCGGTCTCGGTCTCAGCCAGTTCTTCCCTTTTCAGGAGTGTACCAACACGTCAC CGGAGAGATGATGGGTGGCCATGCCATCCGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACTGG GGTGACAATGGTGAGTGGCTGCCCCCTTCCTGCCAAGAACAGTGAATTGTGAGCCACACCCCGTGGCCATCTCGGCTTTCTCTGTTCTACCCACCGCACA GCCTTAAACCCTGGACCTACGGCCAGGCTGTGAGCTCCTCCTAAGTGCCAGGCAGTCAGGAGTTCCCTTTTGCTGTGAGGGCAGACTCTGAGCAGCTTCA GAGCCAACGCCTGCAACAGTTCCCACAGCATCGCGCCAGATCCTGTGATGGGAAGGGTGACCGGGCAGGGGGCTTGCCCGTGGAGGTGTGCCCCACGGCT CCAGAAGCCTTGTGGTGTTGAGGACTGTGCTAGTGGGCCAACAGCAGGAGTGGCCAGGGATGAGTGACTTAAGGTCTTTTAAGGATGAGTCTGACTATAT TGGTTGACCCTTGTCACACTTTAAAAGCACCTTACTTTTTATTCCCAGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGT GGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAATCTGCCGTGGGCCTGTCGTGCCAGTCCTGGGGGCGAGATCGGGGTAGAAATGCA TTTTATTCTTTAAGTTCACGTAAGATACAAGTTTCAGACAGGGTCTGAAGGACTGGATTGGCCAAACATCAGACCTGTCTTCCAAGGAGACCAAGTCCTG GCTACATCCCAGCCTGTGGTTACAGTGCAGACAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCCCCTGTAGACTAGTGCCGTAGGGAGTA CCTGCTGCCCCAGCTGACTGTGGCCCCCTCCGTGATCCATCCATCTCCAGGGAGCAAGACAGAGACGCAGGAATGGAAAGCGGAGTTCCTAACAGGATGA AAGTTCCCCCATCAGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCACAGAAATCAGAGGAGAGACGGTGTTGGGAGCCCTTTGGAGAAC GCCAGTCTCCCAGGCCCCCTGCATCTATCGAGTTTGCAATGTCACAACCTCTCTGATCTTGTGCTCAGCATGATTCTTTAATAGAAGTTTTATTTTTTCG TGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACAGATTGCCTCCTTATGACGCGGCTCAAAAGGAAACCAAG TGGTCAGGAGTTGTTTCTGACCCACTGATCTCTACTACCACAAGGAAAATAGTTTAGGAGAAACCAGCTTTTACTGTTTTTGAAAAATTACAGCTTCACC CTGTCAAGTTAACAAGGAATGCCTGTGCCAATAAAAGTTTTCTCCAACTTGAAGTCTACTCTGATGGGATCTCAGATCCTTTGTCACTGCCTATAGACTT GTAGCTGCTGTCTCTCTTTGTCCCTGCAGAGAATCACGTCCTGGAACTGCATGTTCTTGCGACTCTTGGGACTTCATCTTAACTTCTCGCTGCCCCAGCC ATGTTTTCAACCATGGCATCCCTCCCCCAATTAGTTCCCTGTCATCCTCGTCAACCTTCTCTGTAAGTGCCTGGTAAGCTTGCCCTTGCTTAAGAACTCA AAACATAGCTGTGCTCTATTTTTTTGTTGTTGTTGTGACTGACAGAGTGAGATTCCGTCTCCCAGGCTGGAGTGCAGTGGCGCCTTCTCAGCTCACTGCA ACCTGCAGCCTCCTAGATTCAAGCGATTCTCCTGCTTCAGCCTTCCGAGTAGCTGGGATGACAGGCACTCACCAATATGCCTGGGTAATTTTTGTATTTT TAAGTACATACAGGATTTCACCATGTTGGCCAGGCTAGTTTCAAACTCCCGGCCTCAGGTGGTCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG GCGTGAGCCACTGGGCCCTGCCTGTATTTTTTATCAGCCACAAATCCAGCAACAAGCTGAGGATTCAGCTCATAAAACAGGCTTGGTGTCTTGGTGATCT CACATAACCAAGATGCTACCCCGTGGGGAACCACATCCCCCTGGATGCCCTCCAGCCTTGGTTTGGGCTGGAGTCAGGGCCTGTATACAGTATTTTGAAT TTGTATGCCACTGGTTTGCATTGCTGGTCAGGAACTCTAGTGCTTTGCATAGCCCTGGTTTAGAAACATGTTATAGCAGTTCTTGGTATAGAGCAAACTA GAAGAACCAGCAATCATTCCACTGTCCTGCCAAGGTACACCTCAGTACTCCCCTTCCCAACTGAAGTGGTATGAGGCTAGCTCTTTCCAAAAGCATTCAA GTTTGGCTTCTGATGTGACTCAGAATTTAGGAACCAGATGCTAGATCAAATAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAAGCATCT CTTAAGTGCCATGATGAGCATAACAGCCGGCCGTCGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATGACAAGGTCAGGAGT TCAAGACCAGCCTGGCCAACATGCTGAAACCTCACCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGG AGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGACAGTGCCAGTGCACTCCAGCCTCGGTGACAGCGCAAGGCTCCG TCTCAATAATT

CTSB.F10 (forward primer)
CTSB.R7 (reverse primer)
Intron retention

Frame 0

GAGPGGYLGPGLAQATAAAGLRQPLRQRQPLRC AQAGLQALGCSAGVLLCLPGWCAVVRPRLTAASA TQM*AIWFPPQPPE*ILLKIEMMTLGCKRWLSY V*GDGFSRSQ*LRKFLPDGAALSLVQGVFRPME SSLRGLGLQYLVCIDDWQVDLGSGFQHVAALGLP LLPAGVGQCPEQALFPSPVG*AGQLCQQTEYHV AGRAQLLQRGHELLEEAMWYLPGWAQATPESYV YRGPEAACKLRCTGTMATVSHHQRDQRPGLLWLL LGKALLAGGEALERKWEQHWRVLGDSGWGQL*Q GKL*KLSESQFPSVQKSQSLWP*MMARIRWSGG QSIQQIASPRVQVAKQLPFPDMLARPQIPGALTA TLLQESIPLVSVSCLNFVI*LW*NTRNRNVIIL AIFFCFLFLSFFFFFFFGDGVLLCHPGWSAVVG YWLTATSASWVQAILLPQSPQ*LRLQACATTPS* FLYF**RWGFAMLDSLVSNASPVDLPSSASQNA GITGVSHRAQQLFSSVQFCSIKYIHSVAQPPPP SVPRTLFQLARQKLCPH*HQYCSPW*APLYFLSL *I*LLGTSYKWITTVFIFWVSCVFC*EKNTARY GSSRL*SQHFGRLRQADHLRSGV*DQPGQHGET LSLLKRQNLAGPGGRHL*SQLL*RLRQENCLNPG GVGCSELRLRHCIPAWATKVKLRLQEKKKKPHT HIQTNTTHFADFRNEPSFQAEVHGGASLALKAE LGKSLRQESGASGSSRL*SQHFGRPRWANHLNPG VQDQSGQRSKTPFLPKKIKAEFEPQERPLVLRD QLPTRSEVPLWPFEGTAAEGPARTWQPHPVGLV PAGLRGCGSHL*PDLHPHQCARQRGGVGGGPAHM LWQHVWGRVSQAVLPQRV*C*ETLGPSFSVEGT LRTSWDCCESEVFRGDSESLAGRAWQGCCFLGG A*TKAGPAELSEGWHPSKTVSFRKCGGMSGPWSG LCHRMFLFPAWGTSLGHFLMCS*RGWLHLNNKV VAQNFLLQLPAASPWFRSSGVHWRLQIRNA*GL KKMHGLLP*LVKWESLAILISENWWCF*VP*GSL ASAQLRISVLPGRALVAWSGMDMNRRASCLLPD LCGQGWGWRLFPASCAYLGSVPWRVWGLGGLLA PLAAAGGGSTSGLGHLGSTQWSGELVVLLSEVFI RGCLFSCNGGYPAEAWNFWTRKGLVSGGLYESH VGKCVPLATFWPDGLFEQLTIMALFAFINWGLR QRGC*EVLARCTGLWQDLPGVHAAGTRLALPQVF SVGAVG*LRFLPRPIRVQTVLHPSL*APRQRLP APMHGGGRYPQV*QDL*AWLQPDLQTGQALR*G AWGLATAHVEAGELLHPSSRCSEEVRAEEPWGPG TLG*RRGSDALLPGEAAHSPLSACSTLNSASAL PNWKGPKPSFYKGVACPGVLRYPGLFRGLVLIA PFSHF*ASSPSPVLSSSGEPSLSSPGRASPLRCS LPLEATRNQ*LADRGVPRGTVTALPNPELGSFS RIQFLQRLQ*REGHHGRDLQKRPRGGSFLCVFG LPALQVRCVLMADGNRGWGSGGEPGCQDGSC*PI GLDWAGR*GAGEEGCVGPSI*AHSSVDQPGT*L SLSRCCANTHNSRGLSQSLWASSFPSISVQLIH VLCGNAPLPGGWPRLPSPSWLCIALPACFCYNGR PFVFALADTFSSSFSLQRPRSCLTPVDEHTGRT LPWVVP*GLQPRNLDSPHPQVLS*KSLAPDEQS LLTASIPLIFC*IGNL*NNSLTKEDDSGKKKFL* RWHFLA*SSPLLLKDAFPL*Q*FPHPRAWLGSP EPLSQYLSHQLALAATSPPFSSLTSSGCTWSSY *NSLSRVTLLTNPYLHGIPV*RTDESVI*H*MFY QLECIRNSIFKANKHIL*ESISIFTQQL*VCWA FKNIFNSPVLWDPPWSLLVEQQVPSGSCFSFPE HDQLSVGGAVGRGTVARSRSQPVLPFSGVYQHVT GEMMGGHAIRILGWGVENGTPYWLVANSWNTDW GDNGEWLPPSCQEQ*IVSHTPWPSRLSLFYPPH SLKPWTYGQAVSSS*VPGSQEFPFAVRADSEQLQ SQRLQQFPQHRARSCDGKGDRAGGLPVEVCPTA PEALWC*GLC*WANSRSGQG*VT*GLLRMSLTI LVDPCHTLKAPYFLFPGFFKILRGQDHCGIESEV VAGIPRTDQYWEKI*SAVGLSCQSWGRDRGRNA FYSLSSRKIQVSDRV*RTGLAKHQTCLPRRPSP GYIPACGYSADRPCEPPLPAQSVLPPVD*CRREY LLPQLTVAPSVIHPSPGSKTETQEWKAEFLTG* KFPHQFPQYLQASSFPHLSQKSEERRCWEPFGE RQSPRPPASIEFAMSQPL*SCAQHDSLIEVLFFR ALC*SCG*ASGTAGDLC*FYRLPPYDAAQKETK WSGVVSDPLISTTTRKIV*EKPAFTVFEKLQLH PVKLTRNACANKSFLQLEVYSDGISDPLSLPIDL *LLSLFVPAENHVLELHVLATLGTSS*LLAAPA MFSTMASLPQLVPCHPRQPSL*VPGKLALA*EL KT*LCSIFLLLL*LTE*DSVSQAGVQWRLLSSLQ PAAS*IQAILLLQPSE*LG*QALTNMPG*FLYF *VHTGFHHVGQASFKLPASGGLPASASQSVGIT GVSHWALPVFFISHKSSNKLRIQLIKQAWCLGDL T*PRCYPVGNHIPLDALQPWFGLESGPVYSILN LYATGLHCWSGTLVLCIALV*KHVIAVLGIEQT RRTSNHSTVLPRYTSVLPFPTEVV*G*LFPKAFK FGF*CDSEFRNQMLDQISSENLRNIVGKVC*AS LKCHDEHNSRPSWLTPVIPALWEAKVGG*QGQE FKTSLANMLKPHLY*KYKN*LGMVAHACNPSYLG G*GRRIA*TREAEVAVSQDSASALQPR*QRKAP SQ*

Frame 1

GRGREGT*GRGWPRLRRLQGSGNRSGNANRSAA RRLGCRLSAAALGSCFVSQAGVQWCDHGSRQPQ PPRCKRSGSHLSLPSRYF*K*K**LWDANVGCPM YKEMAFHAPSD*GSFSQMALL*AWCRVSSGLWR AACVGWACSTWFA*MIGRWI*DPASNMWQLWAS LCCLLVLANARSRPSFHPLSDELVNYVNKRNTTW QAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMF TEDLKLPASFDAREQWPQCPTIKEIRDQGSCGS CWVRPCWLAGKRWRESGSNTGESWGIRGGDNSDK ASYRNFLSPSFHQYKNHNPSGHE*WRGLGGVAG RASSRLQVHVYRWRSSSLSLTCWPVRKYQELSL LLCFKKASL*CQ*AVLILSFNCGKIHVTEM**S* QSSFVFFFFLFFFFFFLEMESCSVTQAGVQWWD IGSLQPLPPGFKQFSCLSLPSS*DYRHVPPRPA NFCIFSRDGVLPCWTAWSQTPHLLICLPRPRKML GLQA*ATVPSNYFQVYSSVALSTFTVLLSHHHH LSPELFFSSQDRNSVPINTNIVAPGKPHSTFCL YEFDS*GPHTSGSQQYLFSG*AVFFVKKKTQPGT VAHACNLNTLGG*GRRIT*GQEFETNLANMVKP CLY*KDKT*LGLVAGTYNPSYYRG*GRRIA*TQ EA*VAVS*DCATAFQPGQQK*NSASRKKKKNHTH TYKLILHILQISEMNPVSRQRFTGVLLLL*KLS WANR*GRRVGQVAHHASNPSTLGGQGGQIT*TR EFKTSLANVARPHFYQRK*KLSLSPRSVPWC*EI SCLQGLRCPCGPLRGLLQRAQPGHGSLTRWGSF LQAFGAVEAISDRICIHTNAHVSVEVSAEDLLT CCGSMCGDG*VRLCFHSGFSAERPWAPASQWRGL *GLPGTAASQKCFGETPRVWQAGPGKAAASWEV PEPRLARQSCLRGGIPAKQSVSENVVECLAPGL VCVIACSFFLLGEPVWGISLCVVSGDGSTLTTRW WHRTFCSSCLQPRLGSAVAVFTGGCRSEMPEGL KKCMGFSPN*LNGNHWRFSFQRTGGVFKYLREV *RLLS*ESVFYPEGHS*PGLVWT*TGEPPVFSRI FVGRGGAGGYSLQAVLI*GASLGGFGVWEVCSH HLLQLGVGPRVASGTWVAHSGLESWWCFSQRFS LEAVFSAVMVAILLKLGTSGQEKAWFLVASMNPM *VSVSPWPLSGQMDCLSN*PSWLYLPL*TGG*D RGAAERC*PGAQASGRTCLASMLQARGLPCPRS SPWGL*VDSAFSRVPSGCRPYSIPPCEHHVNGSR PPCTGEGDTPKCSKICEPGYSPTYKQDKHYGKG PGAWPRRTWRLGSCCIPPHAAAKRSGLRSLGVP EL*DRGGGVMPSCQEKQHTLHFLPVPP*TQPQPF QTGRDQSPPFTREWRVLES*GILASSGASCSLL RSPISELPAPALSLVLQGSLP*APLAGQVPLDA VFPWRPPEISDWLTVACHEARSLPSPTPSSVHFP GYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSD FLLYKSGAC*WLMAIEGGGREGSLGARMVHVDQ *GWIGQAGEGLGKRAVWGLPYRLTPLWTSLAPDC LCPGVVPTHTIPEASPRASGLVPSHPLASSSYM FSVGTPPSLVAGHACPRLPGFALLYQHVSVIMA GRSCLHWLTHFLHLSLCKGLDRASHQWMNTQGGP CPG*CHRDSNQGIWTVPIPKSCLRNPLLQMNSL C*LHLSH*YFARLETCKTTV*QRKMILARKSFC EDGTS*HSPRPYY*KMHFHFDSDFPTREPGWAAL SPLASIFPIS*H*LPPVLPSPPSPLQGVPGAAI KTL*AV*RF*QTLTYMESQYKEQMNQSSSIKCF IN*NVLEILFLKPTSTFCRNLFQYLLNNFECVGP SKIYLIPQYCGTPPGASWWSSRFPLEAVSPSRS MTS*VWGVLWGVGQWPGLGLSQFFPFQECTNTS PER*WVAMPSASWAGEWRMAHPTGWLPTPGTLTG VTMVSGCPLPAKNSEL*ATPRGHLGFLCSTHRT ALNPGPTARL*APPKCQAVRSSLLL*GQTLSSF RANACNSSHSIAPDPVMGRVTGQGACPWRCAPRL QKPCGVEDCASGPTAGVARDE*LKVF*G*V*LY WLTLVTL*KHLTFYSQASLKYSEDRITVESNQK WWLEFHAPISTGKRSNLPWACRASPGGEIGVEMH FIL*VHVRYKFQTGSEGLDWPNIRPVFQGDQVL ATSQPVVTVQTGHVSHRCQHRASFPL*TSAVGS TCCPS*LWPPP*SIHLQGARQRRRNGKRSS*QDE SSPISSPSTSKQVAFHICHRNQRRDGVGSPLEN ASLPGPLHLSSLQCHNLSDLVLSMIL**KFYFF VHSANHVGEPVEQRETCASFTDCLLMTRLKRKPS GQELFLTH*SLLPQGK*FRRNQLLLFLKNYSFT LSS*QGMPVPIKVFSNLKSTLMGSQILCHCL*T CSCCLSLSLQRITSWNCMFLRLLGLHLNFSLPQP CFQPWHPSPN*FPVILVNLLCKCLVSLPLLKNS KHSCALFFCCCCD*QSEIPSPRLECSGAFSAHC NLQPPRFKRFSCFSLPSSWDDRHSPICLGNFCIF KYIQDFTMLARLVSNSRPQVVCLPQPPKVLGLQ A*ATGPCLYFLSATNPATS*GFSS*NRLGVLVI SHNQDATPWGTTSPWMPSSLGLGWSQGLYTVF*I CMPLVCIAGQEL*CFA*PWFRNML*QFLV*SKL EEPAIIPLSCQGTPQYSPSQLKWYEASSFQKHS SLASDVTQNLGTRC*IK*ALKI*GTL*ERFVKHL LSAMMSITAGRRGSRL*SQHFGRPRWEDDKVRS SRPAWPTC*NLTSTKNTKISWAWWHMPVIPATW EAEAGESLEPGRRRLQ*AKTVPVHSSLGDSARLR LNN

Frame 2

GGAGRVLRAGAGPGYGGCRAPATAPATPTAPLR AGWAAGSRLQRWGLALSPRLVCSGATTAHGSLS HPDVSDLVPTSASRVDTSENRNDDSGMQTLAVL CIRRWLFTLPVTEEVSPRWRCSEPGAGCLQAYGE QLAWAGPAVPGLHR*LAGGSRIRLPTCGSSGPP SAACWCWPMPGAGPLSIPCRMSWSTMSTNGIPR GRPGTTSTTWT*AT*RGYVVPSWVGPSHPRELCL PRT*SCLQASMHGNNGHSVPPSKRSETRAPVAP AG*GPAGWRGSAGEKVGATLESLGGFGVGTTLT RQVIETF*VPVSISTKITIPLAMNDGED*VEWRA EHPADCKSTCTGGEAAPFP*HAGPSANTRSSHC YSASRKHPFSVSELS*FCHLIVVKYT*QKCNNL SNLLLFSFSFFFFFFFFWRWSLALSPRLECSGGI LAHCNLCLLGSSNSPASVSPVAKTTGMCHHAQL IFVFLVEMGFCHVGQPGLKRLTC*SAFLGLAKC WDYRREPPCPATIFKCTVL*H*VHSQCCSATTTI CPPNSFSARKTETLSPLTPIL*PLVSPTLLSVS MNLTPRDLIQVDHNSIYFLGELCFLLRKKHSQV R*LTPVISTLWEAEAGGSPEVRSLRPTWPTW*NP VSTKKTKLSWAWWQAPIIPATIEAEAGELLEPR RRRLQ*AEIAPLHSSLGNKSETPPPGKKKKTTH THTN*YYTFCRFQK*TQFPGRGSRGCFSCFKS*V GQIVEAGEWGKWLITPLIPALWEAKVGKSLEPG SSRPVWPT*QDPISTKENKS*V*APGASPGVER SVAYKV*GAPVAL*GDCCRGPSPDMAASPGGARS CRPSGLWKPSLTGSASTPMRTSAWRCRRRTCSH AVAACVGTGESGCASTAGLVLRDPGPQLLSGGD FEDFLGLLRVRSVSGRLRESGRQGLARLLLPGRC LNQGWPGRAV*GVASQQNSQFQKMWWNVWPLVW FVSSHVPFSCLGNQFGAFPYV*LAGMAPP*QQG GGTELSAPAACSLALVPQ*RCSLAAADPKCLRA* KNAWASPLTS*MGITGDSHFRELVVFLSTLGKF SVCSVENQCSTRKGTRSLVWYGHEQESLLSSPG SLWAGVGLAVIPCKLCLSRERPLEGLGSGRSART TCCSWGWVHEWPRALG*HTVVWRAGGASLRGFH *RLSFQL*WWLSC*SLELLDKKRPGFWWPL*IP CR*VCPLGHFLARWIV*AINHHGFICLYKLGVET EGLLRGASQVHRPLAGPAWRPCCRHEACLAPGL LRGGCRLTPLSPASHQGADRTPSLPVSTTSTAP GPHARGREIPPSVARSVSLATARPTNRTSTTVRG LGPGHGARGGWGAAASLLTLQRRGQG*GALGSR NSRIEEGE*CPLARRSSTLSTFCLFHPELSLSP SKLEGTKALLLQGSGVSWSLEVSWPLPGPRAHCS VLPFLSFQPQPCP*FFRGAFLELPWQGKSP*MQ SSPGGHQKSVTG*PWRATRHGHCPPQPRARFIF QDTIPTASPIARRTSWPRSTKTAPWRELSLCIRT SCSTSQVRADG*WQ*RVGVGRGAWVPGWFMLTN RAGLGRQVRGWGRGLCGAFHIGSLLCGPAWHLT VFVQVLCQHTQFQRPLPEPLG*FLPIH*RPAHTC SLWERPPPWWLATPALAFLALHCFTSMFLL*WQ AVRVCTG*HIFFIFLFAKA*IVPHTSG*THRED LALGSAIGTPTKESGQSPSPSPVLEIPCSR*TVF ADCIYPINILLDWKLVKQQFDKGR*FWQEKVSV KMALLSIVLALIIKRCISTLTVISPPESLVGQP *AP*PVSFPSASISCHQSSLLLPHLFRVYLEQLL KLFKPCNAFDKPLLTWNPSIKNR*ISHLALNVL SIRMY*KFYF*SQQAHFVGIYFNIYSTTLSVLG LQKYI*FPSIVGPPLEPPGGAAGSLWKLFLLPGA *PAECGGCCGAWDSGPVSVSASSSLFRSVPTRH RRDDGWPCHPHPGLGSGEWHTLLAGCQLLEH*L G*QW*VAAPFLPRTVNCEPHPVAISAFSVLPTAQ P*TLDLRPGCELLLSARQSGVPFCCEGRL*AAS EPTPATVPTASRQIL*WEG*PGRGLARGGVPHG SRSLVVLRTVLVGQQQEWPGMSDLRSFKDESDYI G*PLSHFKSTLLFIPRLL*NTQRTGSLWNRIRS GGWNSTHRSVLGKDLICRGPVVPVLGARSG*KC ILFFKFT*DTSFRQGLKDWIGQTSDLSSKETKSW LHPSLWLQCRQAM*ATAASTERPSPCRLVP*GV PAAPADCGPLRDPSISREQDRDAGMESGVPNRM KVPPSVPPVPPSK*LSTFVTEIRGETVLGALWRT PVSQAPCIYRVCNVTTSLILCSA*FFNRSFIFS CTLLIMWVSQWNSGRPVLVLQIASL*RGSKGNQ VVRSCF*PTDLYYHKENSLGETSFYCF*KITASP CQVNKECLCQ*KFSPT*SLL*WDLRSFVTAYRL VAAVSLCPCRESRPGTACSCDSWDFILTSRCPS HVFNHGIPPPISSLSSSSTFSVSAW*ACPCLRTQ NIAVLYFFVVVVTDRVRFRLPGWSAVAPSQLTA TCSLLDSSDSPASAFRVAGMTGTHQYAWVIFVF LSTYRISPCWPG*FQTPGLRWSACLSLPKCWDYR REPLGPACIFYQPQIQQQAEDSAHKTGLVSW*S HITKMLPRGEPHPPGCPPALVWAGVRACIQYFE FVCHWFALLVRNSSALHSPGLETCYSSSWYRAN* KNQQSFHCPAKVHLSTPLPN*SGMRLALSKSIQ VWLLM*LRI*EPDARSNKL*KSEEHCRKGLLSI S*VP**A*QPAVVAHACNPSTLGGQGGRMTRSGV QDQPGQHAETSPLLKIQKLAGHGGTCL*SQLLG RLRQENRLNPGGGGCSEPRQCQCTPASVTAQGS VSII