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Sequences

Sequence DPH1.aAug05

CTGCGCTCTCCGCGTTCTTCCAGCGCTGTCTTTTTAGTACCACATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGC
CGAGACGGCCCTGGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCAGCAATCCGGG
TCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCT
CCTCTTTGCCTGTACCATTGTGGATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGCTGTGTGGATGAC
TTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTGATTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACG
TCTTTGTGGACATCCGGATAGACACTACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTT
TGTGTCGACCTTGCAGGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGC
ACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCG
CTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTC
AGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCC
TTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTG
ACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTA
CGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCC
GCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTG
CCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAA
TCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCC
TGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCA
AAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCC
ATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCA
AGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCA
GAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGA
CTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAA
AGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCA
GCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTG
AAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTA
GTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACT
ATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

LRSPRSSSAVFLVPHAQAGDGGAGRIRGSGAGR
PRRPWQRSGPSGPRGQSDPP*DPEEPSAAGSNPG
PAFQLQL*DPQDHLEDPTSPGQEGGLANAGRPP
PLCLYHCGYLGKVHGGRSDGDG*RDLRGLLCG*
LHSEGPGS*LLGALRPQLPDSHGHLGPRLPGAVR
LCGHPDRHYTPPGLSPPHLSPSHCPCPGQHHSV
CVDLAGSRPGAESRVSCECPTVQAPVPWRDPGL
HIPPTVQRGGGRCVSWRWPLPSGVCHDCQPQCPR
LPV*PI*QSPIQRTL*PPAHAGCSPRSHSHCPL
S*VLGPYSGHFGPPGQS*DPGAPGISTPSLGPF
LCEAAAL*DLPQQA*PTS*GGCVGAGGMSTSLH*
LGHSLPQAAADTL*GGRGSEGHFLAAALPDGLL
RWQLLGALDGEPRPGPPSPRPGPARAGEGAGGV
RASPFGRGLRGLQLQGREGGAAGSLTRSRASGSC
PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEE
SLGMVAQALNRLGPFDGLLGFSQGAALAALVCA
LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ
RPLSLPSLHVFGDTDKVIPSQESVQLASQFPGA
ITLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KI
KKCLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQ
NQLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH*
LVKTQT*IWLESPGLGG*HGEGRGWHHGDCHII
KW*FGF*ASFSWYTQKNIL*WKSVSWPST*FSGQ
RQAGRCLQL*VLGQAAAWVHILTLLVEVLSPKL
KGRRPRWHNQGGIYI*EVLGRHHLARLPEPQ*F
SLPLSRSLRVVCWSSLKPWMCYLLLPKCSNLAEL
*A*RVGRCQTLGGGQRQYRINSFFLN*P

Frame 1

CALRVLPALSF*YHMRRQVMAALVVSGAAEQGG
RDGPGRGRAPRGRVANQIPPEILKNPQLQAAIR
VLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLL
LFACTIVDILERFTEAEVMVMGDVTYGACCVDD
FTARALGADFLVHYGHSCLIPMDTSAQDFRVLY
VFVDIRIDTTHLLDSLRLTFPPATALALVSTIQF
VSTLQAAAQELKAEYRVSVPQCKPLSPGEILGC
TSPRLSKEVEAVVYLGDGRFHLESVMIANPNVP
AYRYDPYSKVLSREHYDHQRMQAARQEAIATARS
AKSWGLILGTLGRQGSPKILEHLESRLRALGLS
FVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSI
DWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFY
AGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGS
ARPPSAVACEDCSCRDEKVAPLAP*RAPGPQGP
ALRRSSLEAGGFQSRRPTFSPHWKSPPSAGAWRN
HWGWWHRH*TGWGLLTAFLVSAKGLR*QPLCVP
WARQAIPASPCHGLSSWCLVSVPGALGSRNPSC
KGPCHCLRSMFLGTLTKSSPLRRVCNWPANFPEP
SPSPTLVATSFQQLHPSVRPTSSSWTSLQSERS
RNVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLT
RTS*ETTINS*DPNYKGPALY*RKGEHKALMDID
L*KRKHEYGWRALD*EGDMGKAEAGTMVTAT**
SGDLDFEHLFPGTHRKTFYNGSRFLGHLHSFLG
SAKQGGVSSCESSDRLLHGCTYSRYWWKF*VPN*
REGGLGGTIREEYTSEKFWEDITWQGCLNHSNL
VFLYPDH*ELCAGLLSNPGCVTCFSLSALTSLN
YEPEGWVDARP*VEAKDSTE*TASS*IDP

Frame 2

ALSAFFQRCLFSTTCAGR*WRRWSYPGQRSRAA
ETALAEVGPLGAAWPIRSPLRS*RTLSCRQQSG
SCLPTTTLRSPRPSGGSNKPRPRRWPCKCRKAS
SSLPVPLWISWKGSRRPK*W*WVT*PTGLAVWMT
SQRGPWELTSWCTTATVA*FPWTPRPKTSGCCT
SLWTSG*TLHTSWTLSASPFPQPLPLPWSAPFS
LCRPCRQPPRS*KPSIV*VSHSASPCPLERSWAA
HPPDCPKRWRPLCILEMAASIWSLS*LPTPMSP
LTGMTHIAKSYPENTMTTSACRLLAKKP*PLPA
QLSPGALFWALWAARAVLRSWSTWNLDSEPWAFP
L*GCCSLRSSPASLAYFLRWMCGCRWHVHVSPL
TGAQPSPSRC*HPMRRPWL*GTFPGSSPTRWTS
TLAAPWGPGR*TTARTAVPTPRAGPRGGRCRRGP
RVPLRPWLARTAAAGTRRWRRWLLDALPGLRVL
PSGGAASRLVVFRAGGRRFLRIGRARRLQGPGG
ITGDGGTGTEQAGAF*RPSWFQPRGCASSPCVCP
GPGRRSPLPLATVYPLGVWFLSPGHWVQGIHPA
KALVIAFAPCFWGH*QSHPLSGECATGQPISRS
HHPHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQ
EMSLLLHPAPLGAASVIHALPGPSTHCCECASP
EPVKRQLSILETQIIRALPCTEEKGSTRP*WTL
TCENANMNMVGEPWIRRVTWGRQRLAPW*LPHNK
VVIWILSIFFLVHTEKHFIMEVGFLAIYIVFWA
APSREVSPVVSPRTGCCMGAHTHVIGGSFKSQT
EGKEA*VAQSGRNIHLRSFGKTSPGKAA*TTVI*
SSSIQITESCVLVFSQTLDVLPASP*VL*PR*T
MSLKGGSMPDLRWRPKTVQNKQLLPKLTP

Sequence DPH1.cAug05

CTGCGCTCTCCGCGTTCTTCCAGCGCTGTCTTTTTAGTACCACATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGC
CGAGACGGCCCTGGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCAGCAATCCGGG
TCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCT
CCTCTTTGCCTGTACCATTGTGGATATCTTGGAAAGTTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACGTCTTTGTGGACATCCGGATAGA
CACTACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTTTGTGTCGACCTTGCAGGCAGCC
GCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAG
AGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAG
CAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATT
CTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTG
AGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAA
GCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCC
TGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGG
CTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGA
GGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGG
CACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCG
CTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCG
CTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTG
GCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACAT
CCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTAT
CAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAA
TATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGC
ATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTC
CAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGC
ACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACT
GAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGA
TGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

LRSPRSSSAVFLVPHAQAGDGGAGRIRGSGAGR
PRRPWQRSGPSGPRGQSDPP*DPEEPSAAGSNPG
PAFQLQL*DPQDHLEDPTSPGQEGGLANAGRPP
PLCLYHCGYLGKFPWTPRPKTSGCCTSLWTSG*
TLHTSWTLSASPFPQPLPLPWSAPFSLCRPCRQP
PRS*KPSIV*VSHSASPCPLERSWAAHPPDCPK
RWRPLCILEMAASIWSLS*LPTPMSPLTGMTHI
AKSYPENTMTTSACRLLAKKP*PLPAQLSPGALF
WALWAARAVLRSWSTWNLDSEPWAFPL*GCCSL
RSSPASLAYFLRWMCGCRWHVHVSPLTGAQPSP
SRC*HPMRRPWL*GTFPGSSPTRWTSTLAAPWGP
GR*TTARTAVPTPRAGPRGGRCRRGPRVPLRPW
LARTAAAGTRRWRRWLLDALPGLRVLPSGGAAS
RLVVFRAGGRRFLRIGRARRLQGPGGITGDGGTG
TEQAGAF*RPSWFQPRGCASSPCVCPGPGRRSP
LPLATVYPLGVWFLSPGHWVQGIHPAKALVIAF
APCFWGH*QSHPLSGECATGQPISRSHHPHPLWW
PLHSSSCTPASGLPQVLGPVCRVKDQEMSLLLH
PAPLGAASVIHALPGPSTHCCECASPEPVKRQL
SILETQIIRALPCTEEKGSTRP*WTLTCENANMN
MVGEPWIRRVTWGRQRLAPW*LPHNKVVIWILS
IFFLVHTEKHFIMEVGFLAIYIVFWAAPSREVS
PVVSPRTGCCMGAHTHVIGGSFKSQTEGKEA*VA
QSGRNIHLRSFGKTSPGKAA*TTVI*SSSIQIT
ESCVLVFSQTLDVLPASP*VL*PR*TMSLKGGS
MPDLRWRPKTVQNKQLLPKLTP

Frame 1

CALRVLPALSF*YHMRRQVMAALVVSGAAEQGG
RDGPGRGRAPRGRVANQIPPEILKNPQLQAAIR
VLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLL
LFACTIVDILESSHGHLGPRLPGAVRLCGHPDR
HYTPPGLSPPHLSPSHCPCPGQHHSVCVDLAGS
RPGAESRVSCECPTVQAPVPWRDPGLHIPPTVQR
GGGRCVSWRWPLPSGVCHDCQPQCPRLPV*PI*
QSPIQRTL*PPAHAGCSPRSHSHCPLS*VLGPY
SGHFGPPGQS*DPGAPGISTPSLGPFLCEAAAL*
DLPQQA*PTS*GGCVGAGGMSTSLH*LGHSLPQ
AAADTL*GGRGSEGHFLAAALPDGLLRWQLLGA
LDGEPRPGPPSPRPGPARAGEGAGGVRASPFGRG
LRGLQLQGREGGAAGSLTRSRASGSCPPEEQPR
GWWFSEQEADVFSALEEPAVCRGLEESLGMVAQ
ALNRLGPFDGLLGFSQGAALAALVCALGQAGDPR
FPLPRFILLVSGFCPRGIGFKESILQRPLSLPS
LHVFGDTDKVIPSQESVQLASQFPGAITLTHSG
GHFIPAAAPQRQAYLKFLDQFAE*KIKKCLCSYI
QLL*GQPPSSMPSQDPPLTAVSAPHQNQLRDNY
QFLRPKL*GPCPVLKKRGAQGLNGH*LVKTQT*
IWLESPGLGG*HGEGRGWHHGDCHIIKW*FGF*A
SFSWYTQKNIL*WKSVSWPST*FSGQRQAGRCL
QL*VLGQAAAWVHILTLLVEVLSPKLKGRRPRW
HNQGGIYI*EVLGRHHLARLPEPQ*FSLPLSRSL
RVVCWSSLKPWMCYLLLPKCSNLAEL*A*RVGR
CQTLGGGQRQYRINSFFLN*P

Frame 2

ALSAFFQRCLFSTTCAGR*WRRWSYPGQRSRAA
ETALAEVGPLGAAWPIRSPLRS*RTLSCRQQSG
SCLPTTTLRSPRPSGGSNKPRPRRWPCKCRKAS
SSLPVPLWISWKVPMDTSAQDFRVLYVFVDIRID
TTHLLDSLRLTFPPATALALVSTIQFVSTLQAA
AQELKAEYRVSVPQCKPLSPGEILGCTSPRLSK
EVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYS
KVLSREHYDHQRMQAARQEAIATARSAKSWGLI
LGTLGRQGSPKILEHLESRLRALGLSFVRLLLS
EIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPK
PLLTPYEAAVALRDISWQQPYPMDFYAGSSLGP
WTVNHGQDRRPHAPGRPARGKVQEGSARPPSAV
ACEDCSCRDEKVAPLAP*RAPGPQGPALRRSSLE
AGGFQSRRPTFSPHWKSPPSAGAWRNHWGWWHR
H*TGWGLLTAFLVSAKGLR*QPLCVPWARQAIP
ASPCHGLSSWCLVSVPGALGSRNPSCKGPCHCLR
SMFLGTLTKSSPLRRVCNWPANFPEPSPSPTLV
ATSFQQLHPSVRPTSSSWTSLQSERSRNVSAPT
SSSSRGSLRHPCPPRTLHSLL*VRLTRTS*ETTI
NS*DPNYKGPALY*RKGEHKALMDIDL*KRKHE
YGWRALD*EGDMGKAEAGTMVTAT**SGDLDFE
HLFPGTHRKTFYNGSRFLGHLHSFLGSAKQGGVS
SCESSDRLLHGCTYSRYWWKF*VPN*REGGLGG
TIREEYTSEKFWEDITWQGCLNHSNLVFLYPDH
*ELCAGLLSNPGCVTCFSLSALTSLNYEPEGWVD
ARP*VEAKDSTE*TASS*IDP

Sequence DPH1.hAug05

TTCACACGTGAGAAAACCAAGGCTCCAAGAGGTGAAGTGAAGGCTTGTCCCAGTTTACACAGAGGCACAGCACAGCCAGCTGTGAACTCAGTCCTCTGGA
CTTTTTTTTTTTTTTGAGACAGAGTCTTGTTCTGTCGCCCAGGCTGGAGTGCAGAGGTGCGATCTCGGCTCACTGCAGCCTCTGCCTCCCGGGTTCAAGT
GATTATCCTGCTTCAGCCTCCCCAGTAGCTGGGACTACAGGTGCCCGCCACCACGCCCAGCTAAGTTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGT
GTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACTGCACCTGGCAGCCC
TGTGGACTTCTTTTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAAGCTACG
CCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGTCACCACACCCAGCTAAGTTTTTGTATTTTTAGTAGA
GACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCCGCCCGCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA
CCGCGCCCGGCCAGCCCTGTGGACTTCTAAGCCAGCGAGTGCCTCTTCTGCTGCCCTAAACCACACTTAGCCTCCTTTCCACCCCTGCAGGTATCTTGGA
GATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATG
ACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAG
TCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTA
CTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGG
CCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCG
CCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGAC
GAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGC
CGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGAC
GGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCT
TGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAA
AGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCC
CAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGT
CATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGG
GCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGA
GGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAA
ACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGC
TGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGA
GAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCA
AACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAG
ACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

FTREKTKAPRGEVKACPSLHRGTAQPAVNSVLW
TFFFF*DRVLFCRPGWSAEVRSRLTAASASRVQV
IILLQPPQ*LGLQVPATTPS*VFVFLVETGFHR
VSQDGLDLLTS*STRLGLPKCWDYRHEPLHLAA
LWTSFFFFFFLRRSLTLSPRLECSGAISAHCKLR
LPGSRHSPASASRVAGTTGARHHTQLSFCIFSR
DGVSLC*PGWSRSPDLMIRPPRSPKVLGLQA*A
TAPGQPCGLLSQRVPLLLP*TTLSLLSTPAGILE
MAASIWSLS*LPTPMSPLTGMTHIAKSYPENTM
TTSACRLLAKKP*PLPAQLSPGALFWALWAARA
VLRSWSTWNLDSEPWAFPL*GCCSLRSSPASLAY
FLRWMCGCRWHVHVSPLTGAQPSPSRC*HPMRR
PWL*GTFPGSSPTRWTSTLAAPWGPGR*TTART
AVPTPRAGPRGGRCRRGPRVPLRPWLARTAAAGT
RRWRRWLLDALPGLRVLPSGGAASRLVVFRAGG
RRFLRIGRARRLQGPGGITGDGGTGTEQAGAF*
RPSWFQPRGCASSPCVCPGPGRRSPLPLATVYPL
GVWFLSPGHWVQGIHPAKALVIAFAPCFWGH*Q
SHPLSGECATGQPISRSHHPHPLWWPLHSSSCT
PASGLPQVLGPVCRVKDQEMSLLLHPAPLGAASV
IHALPGPSTHCCECASPEPVKRQLSILETQIIR
ALPCTEEKGSTRP*WTLTCENANMNMVGEPWIR
RVTWGRQRLAPW*LPHNKVVIWILSIFFLVHTEK
HFIMEVGFLAIYIVFWAAPSREVSPVVSPRTGC
CMGAHTHVIGGSFKSQTEGKEA*VAQSGRNIHL
RSFGKTSPGKAA*TTVI*SSSIQITESCVLVFSQ
TLDVLPASP*VL*PR*TMSLKGGSMPDLRWRPK
TVQNKQLLPKLTP

Frame 1

SHVRKPRLQEVK*RLVPVYTEAQHSQL*TQSSG
LFFFFETESCSVAQAGVQRCDLGSLQPLPPGFK
*LSCFSLPSSWDYRCPPPRPAKFLYF**RQGFTV
LARMVLIS*PRDPPASASQSVGITGMSHCTWQP
CGLLFFFFSF*DGVSLCHPGWSAVARSQLTASY
ASRVHAILLPQPPE*LGLQAPVTTPS*VFVFLVE
TGFHCVSQDGLDLLTS*SARLGLPKCWDYRREP
PRPASPVDF*ASECLFCCPKPHLASFPPLQVSW
RWPLPSGVCHDCQPQCPRLPV*PI*QSPIQRTL*
PPAHAGCSPRSHSHCPLS*VLGPYSGHFGPPGQ
S*DPGAPGISTPSLGPFLCEAAAL*DLPQQA*P
TS*GGCVGAGGMSTSLH*LGHSLPQAAADTL*GG
RGSEGHFLAAALPDGLLRWQLLGALDGEPRPGP
PSPRPGPARAGEGAGGVRASPFGRGLRGLQLQG
REGGAAGSLTRSRASGSCPPEEQPRGWWFSEQEA
DVFSALEEPAVCRGLEESLGMVAQALNRLGPFD
GLLGFSQGAALAALVCALGQAGDPRFPLPRFIL
LVSGFCPRGIGFKESILQRPLSLPSLHVFGDTDK
VIPSQESVQLASQFPGAITLTHSGGHFIPAAAP
QRQAYLKFLDQFAE*KIKKCLCSYIQLL*GQPP
SSMPSQDPPLTAVSAPHQNQLRDNYQFLRPKL*G
PCPVLKKRGAQGLNGH*LVKTQT*IWLESPGLG
G*HGEGRGWHHGDCHIIKW*FGF*ASFSWYTQK
NIL*WKSVSWPST*FSGQRQAGRCLQL*VLGQAA
AWVHILTLLVEVLSPKLKGRRPRWHNQGGIYI*
EVLGRHHLARLPEPQ*FSLPLSRSLRVVCWSSL
KPWMCYLLLPKCSNLAEL*A*RVGRCQTLGGGQR
QYRINSFFLN*P

Frame 2

HT*ENQGSKR*SEGLSQFTQRHSTASCELSPLD
FFFFLRQSLVLSPRLECRGAISAHCSLCLPGSS
DYPASASPVAGTTGARHHAQLSFCIFSRDRVSP
C*PGWS*SPDLVIHPPRPPKVLGLQA*ATAPGSP
VDFFFFFFLFETESHSVTQAGVQWRDLSSLQAT
PPGFTPFSCLSLPSSWDYRRPSPHPAKFLYF**
RRGFTVLARMVSIS*PHDPPASVSQSAGITGVSH
RARPALWTSKPASASSAALNHT*PPFHPCRYLG
DGRFHLESVMIANPNVPAYRYDPYSKVLSREHY
DHQRMQAARQEAIATARSAKSWGLILGTLGRQGS
PKILEHLESRLRALGLSFVRLLLSEIFPSKLSL
LPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEA
AVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDR
RPHAPGRPARGKVQEGSARPPSAVACEDCSCRD
EKVAPLAP*RAPGPQGPALRRSSLEAGGFQSRR
PTFSPHWKSPPSAGAWRNHWGWWHRH*TGWGLLT
AFLVSAKGLR*QPLCVPWARQAIPASPCHGLSS
WCLVSVPGALGSRNPSCKGPCHCLRSMFLGTLT
KSSPLRRVCNWPANFPEPSPSPTLVATSFQQLHP
SVRPTSSSWTSLQSERSRNVSAPTSSSSRGSLR
HPCPPRTLHSLL*VRLTRTS*ETTINS*DPNYK
GPALY*RKGEHKALMDIDL*KRKHEYGWRALD*E
GDMGKAEAGTMVTAT**SGDLDFEHLFPGTHRK
TFYNGSRFLGHLHSFLGSAKQGGVSSCESSDRL
LHGCTYSRYWWKF*VPN*REGGLGGTIREEYTSE
KFWEDITWQGCLNHSNLVFLYPDH*ELCAGLLS
NPGCVTCFSLSALTSLNYEPEGWVDARP*VEAK
DSTE*TASS*IDP

Sequence DPH1.kAug05

TCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGCAGATGTTATTGTCCCTCCCT
CCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGA
ACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTAGGCGGGGGCTTCCAGGAGGGAGGAGAGACCGCGCCTGGGCACTG
GCCGCCGCCCTGCGACCGCGACGGGAAACGCACAGGAGCGGAGCGGAGGGAAACGCAGGGTCGCAGGAGCGAGACCCTAACCAAAGTCTGCGACCTCAGG
TGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGG
GCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCA
GGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGC
CCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAG
GAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCC
AATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGC
AGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTG
AGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCC
TTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGA
CTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACA
TAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTT
TAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGA
ACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCT
AACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCC
A
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

SVAGDSVPFTGRRED*IQ*SRGRKTLADVIVPP
SPPLGGRGSEGHFLAAALPDGLLRWQLLGALDGE
PRPGPPSPRPGPARAGEGRRGLPGGRRDRAWAL
AAALRPRRETHRSGAEGNAGSQERDPNQSLRPQ
VQEGSARPPSAVACEDCSCRDEKVAPLAP*RAPG
PQGPALRRSSLEAGGFQSRRPTFSPHWKSPPSA
GAWRNHWGWWHRH*TGWGLLTAFLVSAKGLR*Q
PLCVPWARQAIPASPCHGLSSWCLVSVPGALGSR
NPSCKGPCHCLRSMFLGTLTKSSPLRRVCNWPA
NFPEPSPSPTLVATSFQQLHPSVRPTSSSWTSL
QSERSRNVSAPTSSSSRGSLRHPCPPRTLHSLL*
VRLTRTS*ETTINS*DPNYKGPALY*RKGEHKA
LMDIDL*KRKHEYGWRALD*EGDMGKAEAGTMV
TAT**SGDLDFEHLFPGTHRKTFYNGSRFLGHLH
SFLGSAKQGGVSSCESSDRLLHGCTYSRYWWKF
*VPN*REGGLGGTIREEYTSEKFWEDITWQGCL
NHSNLVFLYPDH*ELCAGLLSNPGCVTCFSLSAL
TSLNYEPEGWVDARP*VEAKDSTE*TASS*IDP

Frame 1

LWQGTVCPSQAVVRTEFSNPGAEKHWQMLLSLP
PLP*AAVALRDISWQQPYPMDFYAGSSLGPWTV
NHGQDRRPHAPGRPARGKVGGGFQEGGETAPGHW
PPPCDRDGKRTGAERRETQGRRSETLTKVCDLR
CRRGPRVPLRPWLARTAAAGTRRWRRWLLDALP
GLRVLPSGGAASRLVVFRAGGRRFLRIGRARRLQ
GPGGITGDGGTGTEQAGAF*RPSWFQPRGCASS
PCVCPGPGRRSPLPLATVYPLGVWFLSPGHWVQ
GIHPAKALVIAFAPCFWGH*QSHPLSGECATGQP
ISRSHHPHPLWWPLHSSSCTPASGLPQVLGPVC
RVKDQEMSLLLHPAPLGAASVIHALPGPSTHCC
ECASPEPVKRQLSILETQIIRALPCTEEKGSTRP
*WTLTCENANMNMVGEPWIRRVTWGRQRLAPW*
LPHNKVVIWILSIFFLVHTEKHFIMEVGFLAIY
IVFWAAPSREVSPVVSPRTGCCMGAHTHVIGGSF
KSQTEGKEA*VAQSGRNIHLRSFGKTSPGKAA*
TTVI*SSSIQITESCVLVFSQTLDVLPASP*VL
*PR*TMSLKGGSMPDLRWRPKTVQNKQLLPKLTP

Frame 2

CGRGQCALHRPS*GLNSVIQGQKNTGRCYCPSL
PSPRRPWL*GTFPGSSPTRWTSTLAAPWGPGR*
TTARTAVPTPRAGPRGGR*AGASRREERPRLGT
GRRPATATGNAQERSGGKRRVAGARP*PKSATSG
AGGVRASPFGRGLRGLQLQGREGGAAGSLTRSR
ASGSCPPEEQPRGWWFSEQEADVFSALEEPAVC
RGLEESLGMVAQALNRLGPFDGLLGFSQGAALAA
LVCALGQAGDPRFPLPRFILLVSGFCPRGIGFK
ESILQRPLSLPSLHVFGDTDKVIPSQESVQLAS
QFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQFA
E*KIKKCLCSYIQLL*GQPPSSMPSQDPPLTAV
SAPHQNQLRDNYQFLRPKL*GPCPVLKKRGAQG
LNGH*LVKTQT*IWLESPGLGG*HGEGRGWHHGD
CHIIKW*FGF*ASFSWYTQKNIL*WKSVSWPST
*FSGQRQAGRCLQL*VLGQAAAWVHILTLLVEV
LSPKLKGRRPRWHNQGGIYI*EVLGRHHLARLPE
PQ*FSLPLSRSLRVVCWSSLKPWMCYLLLPKCS
NLAEL*A*RVGRCQTLGGGQRQYRINSFFLN*P

Sequence DPH1.lAug05

TTCACACGTGAGAAAACCAAGGCTCCAAGAGGTGAAGTGAAGGCTTGTCCCAGTTTACACAGAGGCACAGCACAGCCAGCTGTGAACTCAGTCCTCTGGA
CTTTTTTTTTTTTTTGAGACAGAGTCTTGTTCTGTCGCCCAGGCTGGAGTGCAGAGGTGCGATCTCGGCTCACTGCAGCCTCTGCCTCCCGGGTTCAAGT
GATTATCCTGCTTCAGCCTCCCCAGTAGCTGGGACTACAGGTGCCCGCCACCACGCCCAGCTAAGTTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGT
GTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACTGCACCTGGCAGCCC
TGTGGACTTCTTTTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAAGCTACG
CCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGTCACCACACCCAGCTAAGTTTTTGTATTTTTAGTAGA
GACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCCGCCCGCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA
CCGCGCCCGGCCAGCCCTGTGGACTTCTAAGCCAGCGAGTGCCTCTTCTGCTGCCCTAAACCACACTTAGCCTCCTTTCCACCCCTGCAGGTATCTTGGA
GATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATG
ACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCACACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCT
GCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA
GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCT
CCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCC
TTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGG
AGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGAT
GGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCA
GGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGT
CATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCAC
CCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTC
TGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAA
GAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAAC
GCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTT
GGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAG
GGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAG
GCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTA
TCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAA
GGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

FTREKTKAPRGEVKACPSLHRGTAQPAVNSVLW
TFFFF*DRVLFCRPGWSAEVRSRLTAASASRVQV
IILLQPPQ*LGLQVPATTPS*VFVFLVETGFHR
VSQDGLDLLTS*STRLGLPKCWDYRHEPLHLAA
LWTSFFFFFFLRRSLTLSPRLECSGAISAHCKLR
LPGSRHSPASASRVAGTTGARHHTQLSFCIFSR
DGVSLC*PGWSRSPDLMIRPPRSPKVLGLQA*A
TAPGQPCGLLSQRVPLLLP*TTLSLLSTPAGILE
MAASIWSLS*LPTPMSPLTGMTHIAKSYPENTM
TTSACRLLAKKP*PLPAHTWNLDSEPWAFPL*G
CCSLRSSPASLAYFLRWMCGCRWHVHVSPLTGAQ
PSPSRC*HPMRRPWL*GTFPGSSPTRWTSTLAA
PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVP
LRPWLARTAAAGTRRWRRWLLDALPGLRVLPSGG
AASRLVVFRAGGRRFLRIGRARRLQGPGGITGD
GGTGTEQAGAF*RPSWFQPRGCASSPCVCPGPG
RRSPLPLATVYPLGVWFLSPGHWVQGIHPAKALV
IAFAPCFWGH*QSHPLSGECATGQPISRSHHPH
PLWWPLHSSSCTPASGLPQVLGPVCRVKDQEMS
LLLHPAPLGAASVIHALPGPSTHCCECASPEPVK
RQLSILETQIIRALPCTEEKGSTRP*WTLTCEN
ANMNMVGEPWIRRVTWGRQRLAPW*LPHNKVVI
WILSIFFLVHTEKHFIMEVGFLAIYIVFWAAPSR
EVSPVVSPRTGCCMGAHTHVIGGSFKSQTEGKE
A*VAQSGRNIHLRSFGKTSPGKAA*TTVI*SSS
IQITESCVLVFSQTLDVLPASP*VL*PR*TMSLK
GGSMPDLRWRPKTVQNKQLLPKLTP

Frame 1

SHVRKPRLQEVK*RLVPVYTEAQHSQL*TQSSG
LFFFFETESCSVAQAGVQRCDLGSLQPLPPGFK
*LSCFSLPSSWDYRCPPPRPAKFLYF**RQGFTV
LARMVLIS*PRDPPASASQSVGITGMSHCTWQP
CGLLFFFFSF*DGVSLCHPGWSAVARSQLTASY
ASRVHAILLPQPPE*LGLQAPVTTPS*VFVFLVE
TGFHCVSQDGLDLLTS*SARLGLPKCWDYRREP
PRPASPVDF*ASECLFCCPKPHLASFPPLQVSW
RWPLPSGVCHDCQPQCPRLPV*PI*QSPIQRTL*
PPAHAGCSPRSHSHCPLTPGISTPSLGPFLCEA
AAL*DLPQQA*PTS*GGCVGAGGMSTSLH*LGH
SLPQAAADTL*GGRGSEGHFLAAALPDGLLRWQL
LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP
FGRGLRGLQLQGREGGAAGSLTRSRASGSCPPE
EQPRGWWFSEQEADVFSALEEPAVCRGLEESLGM
VAQALNRLGPFDGLLGFSQGAALAALVCALGQA
GDPRFPLPRFILLVSGFCPRGIGFKESILQRPL
SLPSLHVFGDTDKVIPSQESVQLASQFPGAITLT
HSGGHFIPAAAPQRQAYLKFLDQFAE*KIKKCL
CSYIQLL*GQPPSSMPSQDPPLTAVSAPHQNQL
RDNYQFLRPKL*GPCPVLKKRGAQGLNGH*LVKT
QT*IWLESPGLGG*HGEGRGWHHGDCHIIKW*F
GF*ASFSWYTQKNIL*WKSVSWPST*FSGQRQA
GRCLQL*VLGQAAAWVHILTLLVEVLSPKLKGRR
PRWHNQGGIYI*EVLGRHHLARLPEPQ*FSLPL
SRSLRVVCWSSLKPWMCYLLLPKCSNLAEL*A*
RVGRCQTLGGGQRQYRINSFFLN*P

Frame 2

HT*ENQGSKR*SEGLSQFTQRHSTASCELSPLD
FFFFLRQSLVLSPRLECRGAISAHCSLCLPGSS
DYPASASPVAGTTGARHHAQLSFCIFSRDRVSP
C*PGWS*SPDLVIHPPRPPKVLGLQA*ATAPGSP
VDFFFFFFLFETESHSVTQAGVQWRDLSSLQAT
PPGFTPFSCLSLPSSWDYRRPSPHPAKFLYF**
RRGFTVLARMVSIS*PHDPPASVSQSAGITGVSH
RARPALWTSKPASASSAALNHT*PPFHPCRYLG
DGRFHLESVMIANPNVPAYRYDPYSKVLSREHY
DHQRMQAARQEAIATARSHLESRLRALGLSFVRL
LLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGT
AFPKPLLTPYEAAVALRDISWQQPYPMDFYAGS
SLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP
SAVACEDCSCRDEKVAPLAP*RAPGPQGPALRR
SSLEAGGFQSRRPTFSPHWKSPPSAGAWRNHWG
WWHRH*TGWGLLTAFLVSAKGLR*QPLCVPWARQ
AIPASPCHGLSSWCLVSVPGALGSRNPSCKGPC
HCLRSMFLGTLTKSSPLRRVCNWPANFPEPSPS
PTLVATSFQQLHPSVRPTSSSWTSLQSERSRNVS
APTSSSSRGSLRHPCPPRTLHSLL*VRLTRTS*
ETTINS*DPNYKGPALY*RKGEHKALMDIDL*K
RKHEYGWRALD*EGDMGKAEAGTMVTAT**SGDL
DFEHLFPGTHRKTFYNGSRFLGHLHSFLGSAKQ
GGVSSCESSDRLLHGCTYSRYWWKF*VPN*REG
GLGGTIREEYTSEKFWEDITWQGCLNHSNLVFLY
PDH*ELCAGLLSNPGCVTCFSLSALTSLNYEPE
GWVDARP*VEAKDSTE*TASS*IDP

Sequence DPH1.mAug05

TCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCA
TGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCA
AGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTC
AGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAGGTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCT
GCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCC
TACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGC
GGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGAC
CGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGG
ACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAG
GCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTG
ACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCT
CTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGAC
AAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCAC
CCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCC
GTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAA
GGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAG
GAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAA
AAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCT
GCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCT
GAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCT
CAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAA
AGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

SPGEILGCTSPRLSKEVEAVVYLGDGRFHLESV
MIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQ
EAIATARSAKSWGLILGTLGRQGSPKILEVSGL
RTASGGGK*LGTQLGKPVGHRFSFGCLTR*PPP
CLPLPTAPGISTPSLGPFLCEAAAL*DLPQQA*P
TS*GGCVGAGGMSTSLH*LGHSLPQAAADTL*G
GRGSEGHFLAAALPDGLLRWQLLGALDGEPRPG
PPSPRPGPARAGEGAGGVRASPFGRGLRGLQLQG
REGGAAGSLTRSRASGSCPPEEQPRGWWFSEQE
ADVFSALEEPAVCRGLEESLGMVAQALNRLGPF
DGLLGFSQGAALAALVCALGQAGDPRFPLPRFIL
LVSGFCPRGIGFKESILQRPLSLPSLHVFGDTD
KVIPSQESVQLASQFPGAITLTHSGGHFIPAAA
PQRQAYLKFLDQFAE*KIKKCLCSYIQLL*GQPP
SSMPSQDPPLTAVSAPHQNQLRDNYQFLRPKL*
GPCPVLKKRGAQGLNGH*LVKTQT*IWLESPGL
GG*HGEGRGWHHGDCHIIKW*FGF*ASFSWYTQK
NIL*WKSVSWPST*FSGQRQAGRCLQL*VLGQA
AAWVHILTLLVEVLSPKLKGRRPRWHNQGGIYI
*EVLGRHHLARLPEPQ*FSLPLSRSLRVVCWSSL
KPWMCYLLLPKCSNLAEL*A*RVGRCQTLGGGQ
RQYRINSFFLN*P

Frame 1

PLERSWAAHPPDCPKRWRPLCILEMAASIWSLS
*LPTPMSPLTGMTHIAKSYPENTMTTSACRLLA
KKP*PLPAQLSPGALFWALWAARAVLRSWRSVGS
GQPLEEGSDWEHSWENQ*ATGSALAA*PGDPHP
ASLSQQHLESRLRALGLSFVRLLLSEIFPSKLS
LLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEA
AVALRDISWQQPYPMDFYAGSSLGPWTVNHGQD
RRPHAPGRPARGKVQEGSARPPSAVACEDCSCR
DEKVAPLAP*RAPGPQGPALRRSSLEAGGFQSRR
PTFSPHWKSPPSAGAWRNHWGWWHRH*TGWGLL
TAFLVSAKGLR*QPLCVPWARQAIPASPCHGLS
SWCLVSVPGALGSRNPSCKGPCHCLRSMFLGTLT
KSSPLRRVCNWPANFPEPSPSPTLVATSFQQLH
PSVRPTSSSWTSLQSERSRNVSAPTSSSSRGSL
RHPCPPRTLHSLL*VRLTRTS*ETTINS*DPNYK
GPALY*RKGEHKALMDIDL*KRKHEYGWRALD*
EGDMGKAEAGTMVTAT**SGDLDFEHLFPGTHR
KTFYNGSRFLGHLHSFLGSAKQGGVSSCESSDRL
LHGCTYSRYWWKF*VPN*REGGLGGTIREEYTS
EKFWEDITWQGCLNHSNLVFLYPDH*ELCAGLL
SNPGCVTCFSLSALTSLNYEPEGWVDARP*VEAK
DSTE*TASS*IDP

Frame 2

PWRDPGLHIPPTVQRGGGRCVSWRWPLPSGVCH
DCQPQCPRLPV*PI*QSPIQRTL*PPAHAGCSP
RSHSHCPLS*VLGPYSGHFGPPGQS*DPGGQWA
QDSLWRREVTGNTAGKTSRPQVQLWLLDQVTPTL
PPSPNSTWNLDSEPWAFPL*GCCSLRSSPASLA
YFLRWMCGCRWHVHVSPLTGAQPSPSRC*HPMR
RPWL*GTFPGSSPTRWTSTLAAPWGPGR*TTART
AVPTPRAGPRGGRCRRGPRVPLRPWLARTAAAG
TRRWRRWLLDALPGLRVLPSGGAASRLVVFRAG
GRRFLRIGRARRLQGPGGITGDGGTGTEQAGAF*
RPSWFQPRGCASSPCVCPGPGRRSPLPLATVYP
LGVWFLSPGHWVQGIHPAKALVIAFAPCFWGH*
QSHPLSGECATGQPISRSHHPHPLWWPLHSSSCT
PASGLPQVLGPVCRVKDQEMSLLLHPAPLGAAS
VIHALPGPSTHCCECASPEPVKRQLSILETQII
RALPCTEEKGSTRP*WTLTCENANMNMVGEPWIR
RVTWGRQRLAPW*LPHNKVVIWILSIFFLVHTE
KHFIMEVGFLAIYIVFWAAPSREVSPVVSPRTG
CCMGAHTHVIGGSFKSQTEGKEA*VAQSGRNIHL
RSFGKTSPGKAA*TTVI*SSSIQITESCVLVFS
QTLDVLPASP*VL*PR*TMSLKGGSMPDLRWRP
KTVQNKQLLPKLTP

Sequence DPH1.eAug05

CTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTT
CCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTG
CTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGG
TGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGA
GGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGGGTGCGCCCCGCCTTTTG
CCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCTTCCGCTCGGGGAAAGAC
CGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCGGCAGAGCGAGCGGGGCT
TCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCCGACCCCCCGGGCCCCGA
GGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGACACCCTTCAGCATCCCCC
ACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGCCTGTCCTCCCAGGCTTC
CGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCTCGATTCCCTTTTTCTCT
CTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCATATCGCTGTAGGGTCCTG
CCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAA
TCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCC
TGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCA
AAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCC
ATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCA
AGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCA
GAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGA
CTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAA
AGTGGTGATTTGG
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

LWAARAVLRSWSTWNLDSEPWAFPL*GCCSLRS
SPASLAYFLRWMCGCRWHVHVSPLTGAQPSPSRC
*HPMRRPWL*GTFPGSSPTRWTSTLAAPWGPGR
*TTARTAVPTPRAGPRGGRCRRGPRVPLRPWLA
RTAAAGTRRWRRWLLDALPGLRYQPPLWVRPAFC
RCHGNALALRGPPSGRC*RSVPPRALCFRSGKD
RFRCFRRSLPGIMAAQRPLRVLCLAGFRQSERG
FREKTGALRKALRGRAELVCLSGPHPVPDPPGPE
GARSDFGETSPAPETHSVSSILFRPPRHPSASP
TLRPPAFLPPSPHPSFLATHTLFAQTAACPPRL
PPSPSSSAVSCSGQSTHFPPLISPRLLPRFPFSL
CHLEPSCPFSFLSFLEPPMP*SHPFSSYRCRVL
PSGGAASRLVVFRAGGRRFLRIGRARRLQGPGG
ITGDGGTGTEQAGAF*RPSWFQPRGCASSPCVCP
GPGRRSPLPLATVYPLGVWFLSPGHWVQGIHPA
KALVIAFAPCFWGH*QSHPLSGECATGQPISRS
HHPHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQ
EMSLLLHPAPLGAASVIHALPGPSTHCCECASP
EPVKRQLSILETQIIRALPCTEEKGSTRP*WTL
TCENANMNMVGEPWIRRVTWGRQRLAPW*LPHNK
VVIW

Frame 1

FGPPGQS*DPGAPGISTPSLGPFLCEAAAL*DL
PQQA*PTS*GGCVGAGGMSTSLH*LGHSLPQAA
ADTL*GGRGSEGHFLAAALPDGLLRWQLLGALDG
EPRPGPPSPRPGPARAGEGAGGVRASPFGRGLR
GLQLQGREGGAAGSLTRSRASGISPRSGCAPPF
AVVMGTPWRSEARPRGGADVRFRPVHCASARGKT
ASGASVAPCRA*WPRSDPCGSCAWRASGRASGA
SVRRPGR*GRRCGVAPSSCASAARTRSPTPRAP
RAPDQTSVRQAPLRKRTQFPPSCFVPLDTLQHPP
PCVHPHSSHPVHTLHFSRPIPSLLKLQPVLPGF
RLHRLPPLSPVQANPLISPLSFPPDFCLDSLFL
SVISNPPALSHFFPSWSLPCPNPILFPHIAVGSC
PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEE
SLGMVAQALNRLGPFDGLLGFSQGAALAALVCA
LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ
RPLSLPSLHVFGDTDKVIPSQESVQLASQFPGA
ITLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KI
KKCLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQ
NQLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH*
LVKTQT*IWLESPGLGG*HGEGRGWHHGDCHII
KW*F

Frame 2

LGRQGSPKILEHLESRLRALGLSFVRLLLSEIF
PSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPL
LTPYEAAVALRDISWQQPYPMDFYAGSSLGPWT
VNHGQDRRPHAPGRPARGKVQEGSARPPSAVACE
DCSCRDEKVAPLAP*RAPGPQVSAPALGAPRLL
PLSWERLGAPRPALGAVLTFGSAPCIVLPLGER
PLPVLPSLLAGHNGRAATPAGPVPGGLPAERAGL
P*EDRGAEEGAAGSRRARVPQRPAPGPRPPGPR
GRQIRLR*DKPRSGNALSFLHLVSSPSTPFSIP
HPASTRIPPTQSTPFISRDPYPLCSNCSLSSQAS
AFTVFLRCLLFRPIHSFPPSHFPQTFASIPFFS
LSSRTLLPFLISFLPGASHALIPSFFLISL*GP
ALRRSSLEAGGFQSRRPTFSPHWKSPPSAGAWRN
HWGWWHRH*TGWGLLTAFLVSAKGLR*QPLCVP
WARQAIPASPCHGLSSWCLVSVPGALGSRNPSC
KGPCHCLRSMFLGTLTKSSPLRRVCNWPANFPEP
SPSPTLVATSFQQLHPSVRPTSSSWTSLQSERS
RNVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLT
RTS*ETTINS*DPNYKGPALY*RKGEHKALMDID
L*KRKHEYGWRALD*EGDMGKAEAGTMVTAT**
SGDL

Sequence DPH1.fAug05

GCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTCAGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAG
GTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCTGCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAG
GCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGAGTATCTGCCTGGCTATGACTGGCTAAAGAAGCTTAGAAG
CTGGGTCTTGCCCAGCTCGTCTTGAAGGCTGGAGCCAACAGGAATTGCTTCCATGGTCATGGAATTTTACTGTTTACAGAGACCTCCCTGGGAGGGCTAA
CTGGGTCATACCTGCTTTGGGACTTTGGGGCTCAGAGGCAATGGCAACATCACATTTGTCCTTGTGAGATGAGTTTGAGCGGTGGCTGACACCTGCCCTG
TACCAGGTACTAAATGTGGTTCTGGGGATACTATCACCAGTGAGCTGCCCTGGAAAGCTCATTGTCGGCAGAGGAAACAGGAGAAAACACGCAACAATAC
AGTATTCCAAACAGCAGTGGGTCACTGTCTTTACTTTAGGATTCATAAAGCCTGTTAATGAATGGGTGTGCTGGGGGCACTGTCATGTTCTTCAGCATGC
TGCCTGGGCGACGAGGAGGCCCTTCCTAGGGTCTGACCTGGCTTCCCTTCCCAGGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA
GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGTAACACCAAGCTCTGGGAGAGAGTGGGCTTTGGACGTGGTTCTCAAAGGTGAGGTCTGGAGTGGAG
TGGGGTGGGTGGGCAGCACTTCGTTATGTTCGTAGATTCCGGAGTCTGTCTCGATTTCTCCAGCGGAGTCACTTCCTAGCTGTGTAGCCTTAGGCAAGGC
CCTGAACCACACAGTTCCCTTCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGC
AGATGTTATTGTCCCTCCCTCCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTC
CTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCT
TCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGG
GTGCGCCCCGCCTTTTGCCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCT
TCCGCTCGGGGAAAGACCGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCG
GCAGAGCGAGCGGGGCTTCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCC
GACCCCCCGGGCCCCGAGGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGAC
ACCCTTCAGCATCCCCCACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGC
CTGTCCTCCCAGGCTTCCGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCT
CGATTCCCTTTTTCTCTCTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCAT
ATCGCTGTAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCT
GCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGC
AGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTC
AAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCA
GCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTT
TGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCT
GTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAG
GCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGG
TGACTGCCACATAATAAAGTGGTGATTTGG
  • DPH1.F1 (forward primer)
  • DPH1.R1 (reverse primer)
  • Intron retention

Frame 0

ARAVLRSWRSVGSGQPLEEGSDWEHSWENQ*AT
GSALAA*PGDPHPASLSQQHLESRLRALGLSFVR
LLLSEIFPSKLSLLPEVDV*VSAWL*LAKEA*K
LGLAQLVLKAGANRNCFHGHGILLFTETSLGGL
TGSYLLWDFGAQRQWQHHICPCEMSLSGG*HLPC
TRY*MWFWGYYHQ*AALESSLSAEETGENTQQY
SIPNSSGSLSLL*DS*SLLMNGCAGGTVMFFSM
LPGRRGGPS*GLTWLPFPGGCRWHVHVSPLTGAQ
PSPSRC*HPMR*HQALGESGLWTWFSKVRSGVE
WGGWAALRYVRRFRSLSRFLQRSHFLAV*P*AR
P*TTQFPSVAGDSVPFTGRRED*IQ*SRGRKTLA
DVIVPPSPPLGGRGSEGHFLAAALPDGLLRWQL
LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP
FGRGLRGLQLQGREGGAAGSLTRSRASGISPRSG
CAPPFAVVMGTPWRSEARPRGGADVRFRPVHCA
SARGKTASGASVAPCRA*WPRSDPCGSCAWRAS
GRASGASVRRPGR*GRRCGVAPSSCASAARTRSP
TPRAPRAPDQTSVRQAPLRKRTQFPPSCFVPLD
TLQHPPPCVHPHSSHPVHTLHFSRPIPSLLKLQ
PVLPGFRLHRLPPLSPVQANPLISPLSFPPDFCL
DSLFLSVISNPPALSHFFPSWSLPCPNPILFPH
IAVGSCPPEEQPRGWWFSEQEADVFSALEEPAV
CRGLEESLGMVAQALNRLGPFDGLLGFSQGAALA
ALVCALGQAGDPRFPLPRFILLVSGFCPRGIGF
KESILQRPLSLPSLHVFGDTDKVIPSQESVQLA
SQFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQF
AE*KIKKCLCSYIQLL*GQPPSSMPSQDPPLTA
VSAPHQNQLRDNYQFLRPKL*GPCPVLKKRGAQ
GLNGH*LVKTQT*IWLESPGLGG*HGEGRGWHHG
DCHIIKW*F

Frame 1

PGQS*DPGGQWAQDSLWRREVTGNTAGKTSRPQ
VQLWLLDQVTPTLPPSPNSTWNLDSEPWAFPL*
GCCSLRSSPASLAYFLRWMCEYLPGYDWLKKLRS
WVLPSSS*RLEPTGIASMVMEFYCLQRPPWEG*
LGHTCFGTLGLRGNGNITFVLVR*V*AVADTCP
VPGTKCGSGDTITSELPWKAHCRQRKQEKTRNNT
VFQTAVGHCLYFRIHKAC**MGVLGALSCSSAC
CLGDEEALPRV*PGFPSQVGAGGMSTSLH*LGH
SLPQAAADTL*GNTKLWERVGFGRGSQR*GLEWS
GVGGQHFVMFVDSGVCLDFSSGVTS*LCSLRQG
PEPHSSLLWQGTVCPSQAVVRTEFSNPGAEKHW
QMLLSLPPLP*AAVALRDISWQQPYPMDFYAGSS
LGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP
SAVACEDCSCRDEKVAPLAP*RAPGPQVSAPAL
GAPRLLPLSWERLGAPRPALGAVLTFGSAPCIVL
PLGERPLPVLPSLLAGHNGRAATPAGPVPGGLP
AERAGLP*EDRGAEEGAAGSRRARVPQRPAPGP
RPPGPRGRQIRLR*DKPRSGNALSFLHLVSSPST
PFSIPHPASTRIPPTQSTPFISRDPYPLCSNCS
LSSQASAFTVFLRCLLFRPIHSFPPSHFPQTFA
SIPFFSLSSRTLLPFLISFLPGASHALIPSFFLI
SL*GPALRRSSLEAGGFQSRRPTFSPHWKSPPS
AGAWRNHWGWWHRH*TGWGLLTAFLVSAKGLR*
QPLCVPWARQAIPASPCHGLSSWCLVSVPGALGS
RNPSCKGPCHCLRSMFLGTLTKSSPLRRVCNWP
ANFPEPSPSPTLVATSFQQLHPSVRPTSSSWTS
LQSERSRNVSAPTSSSSRGSLRHPCPPRTLHSLL
*VRLTRTS*ETTINS*DPNYKGPALY*RKGEHK
ALMDIDL*KRKHEYGWRALD*EGDMGKAEAGTM
VTAT**SGDL

Frame 2

QGSPKILEVSGLRTASGGGK*LGTQLGKPVGHR
FSFGCLTR*PPPCLPLPTAPGISTPSLGPFLCE
AAAL*DLPQQA*PTS*GGCVSICLAMTG*RSLE
AGSCPARLEGWSQQELLPWSWNFTVYRDLPGRAN
WVIPALGLWGSEAMATSHLSL*DEFERWLTPAL
YQVLNVVLGILSPVSCPGKLIVGRGNRRKHATI
QYSKQQWVTVFTLGFIKPVNEWVCWGHCHVLQHA
AWATRRPFLGSDLASLPRWVQVACPRLSIDWGT
AFPKPLLTPYEVTPSSGREWALDVVLKGEVWSG
VGWVGSTSLCS*IPESVSISPAESLPSCVALGKA
LNHTVPFCGRGQCALHRPS*GLNSVIQGQKNTG
RCYCPSLPSPRRPWL*GTFPGSSPTRWTSTLAA
PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVPL
RPWLARTAAAGTRRWRRWLLDALPGLRYQPPLW
VRPAFCRCHGNALALRGPPSGRC*RSVPPRALC
FRSGKDRFRCFRRSLPGIMAAQRPLRVLCLAGFR
QSERGFREKTGALRKALRGRAELVCLSGPHPVP
DPPGPEGARSDFGETSPAPETHSVSSILFRPPR
HPSASPTLRPPAFLPPSPHPSFLATHTLFAQTAA
CPPRLPPSPSSSAVSCSGQSTHFPPLISPRLLP
RFPFSLCHLEPSCPFSFLSFLEPPMP*SHPFSS
YRCRVLPSGGAASRLVVFRAGGRRFLRIGRARRL
QGPGGITGDGGTGTEQAGAF*RPSWFQPRGCAS
SPCVCPGPGRRSPLPLATVYPLGVWFLSPGHWV
QGIHPAKALVIAFAPCFWGH*QSHPLSGECATGQ
PISRSHHPHPLWWPLHSSSCTPASGLPQVLGPV
CRVKDQEMSLLLHPAPLGAASVIHALPGPSTHC
CECASPEPVKRQLSILETQIIRALPCTEEKGSTR
P*WTLTCENANMNMVGEPWIRRVTWGRQRLAPW
*LPHNKVVIW

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