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Sequences

Sequence DPH1.gAug05

GCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTCAGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAG GTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCTGCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAG GCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGAGTATCTGCCTGGCTATGACTGGCTAAAGAAGCTTAGAAG CTGGGTCTTGCCCAGCTCGTCTTGAAGGCTGGAGCCAACAGGAATTGCTTCCATGGTCATGGAATTTTACTGTTTACAGAGACCTCCCTGGGAGGGCTAA CTGGGTCATACCTGCTTTGGGACTTTGGGGCTCAGAGGCAATGGCAACATCACATTTGTCCTTGTGAGATGAGTTTGAGCGGTGGCTGACACCTGCCCTG TACCAGGTACTAAATGTGGTTCTGGGGATACTATCACCAGTGAGCTGCCCTGGAAAGCTCATTGTCGGCAGAGGAAACAGGAGAAAACACGCAACAATAC AGTATTCCAAACAGCAGTGGGTCACTGTCTTTACTTTAGGATTCATAAAGCCTGTTAATGAATGGGTGTGCTGGGGGCACTGTCATGTTCTTCAGCATGC TGCCTGGGCGACGAGGAGGCCCTTCCTAGGGTCTGACCTGGCTTCCCTTCCCAGGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGTAACACCAAGCTCTGGGAGAGAGTGGGCTTTGGACGTGGTTCTCAAAGGTGAGGTCTGGAGTGGAG TGGGGTGGGTGGGCAGCACTTCGTTATGTTCGTAGATTCCGGAGTCTGTCTCGATTTCTCCAGCGGAGTCACTTCCTAGCTGTGTAGCCTTAGGCAAGGC CCTGAACCACACAGTTCCCTTCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGC AGATGTTATTGTCCCTCCCTCCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTC CTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCT TCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGG GTGCGCCCCGCCTTTTGCCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCT TCCGCTCGGGGAAAGACCGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCG GCAGAGCGAGCGGGGCTTCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCC GACCCCCCGGGCCCCGAGGGCGCCAGATCAGACTTCGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTT TCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCT TGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCT GGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCC CCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCA GGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATG CCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCC CTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACA TGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTA TAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGG TGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTT GGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGG ATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACA GAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F1 (forward primer)
DPH1.R1 (reverse primer)
Intron retention

Frame 0

ARAVLRSWRSVGSGQPLEEGSDWEHSWENQ*AT GSALAA*PGDPHPASLSQQHLESRLRALGLSFVR LLLSEIFPSKLSLLPEVDV*VSAWL*LAKEA*K LGLAQLVLKAGANRNCFHGHGILLFTETSLGGL TGSYLLWDFGAQRQWQHHICPCEMSLSGG*HLPC TRY*MWFWGYYHQ*AALESSLSAEETGENTQQY SIPNSSGSLSLL*DS*SLLMNGCAGGTVMFFSM LPGRRGGPS*GLTWLPFPGGCRWHVHVSPLTGAQ PSPSRC*HPMR*HQALGESGLWTWFSKVRSGVE WGGWAALRYVRRFRSLSRFLQRSHFLAV*P*AR P*TTQFPSVAGDSVPFTGRRED*IQ*SRGRKTLA DVIVPPSPPLGGRGSEGHFLAAALPDGLLRWQL LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP FGRGLRGLQLQGREGGAAGSLTRSRASGISPRSG CAPPFAVVMGTPWRSEARPRGGADVRFRPVHCA SARGKTASGASVAPCRA*WPRSDPCGSCAWRAS GRASGASVRRPGR*GRRCGVAPSSCASAARTRSP TPRAPRAPDQTSGPALRRSSLEAGGFQSRRPTF SPHWKSPPSAGAWRNHWGWWHRH*TGWGLLTAF LVSAKGLR*QPLCVPWARQAIPASPCHGLSSWCL VSVPGALGSRNPSCKGPCHCLRSMFLGTLTKSS PLRRVCNWPANFPEPSPSPTLVATSFQQLHPSV RPTSSSWTSLQSERSRNVSAPTSSSSRGSLRHPC PPRTLHSLL*VRLTRTS*ETTINS*DPNYKGPA LY*RKGEHKALMDIDL*KRKHEYGWRALD*EGD MGKAEAGTMVTAT**SGDLDFEHLFPGTHRKTFY NGSRFLGHLHSFLGSAKQGGVSSCESSDRLLHG CTYSRYWWKF*VPN*REGGLGGTIREEYTSEKF WEDITWQGCLNHSNLVFLYPDH*ELCAGLLSNPG CVTCFSLSALTSLNYEPEGWVDARP*VEAKDST E*TASS*IDP

Frame 1

PGQS*DPGGQWAQDSLWRREVTGNTAGKTSRPQ VQLWLLDQVTPTLPPSPNSTWNLDSEPWAFPL* GCCSLRSSPASLAYFLRWMCEYLPGYDWLKKLRS WVLPSSS*RLEPTGIASMVMEFYCLQRPPWEG* LGHTCFGTLGLRGNGNITFVLVR*V*AVADTCP VPGTKCGSGDTITSELPWKAHCRQRKQEKTRNNT VFQTAVGHCLYFRIHKAC**MGVLGALSCSSAC CLGDEEALPRV*PGFPSQVGAGGMSTSLH*LGH SLPQAAADTL*GNTKLWERVGFGRGSQR*GLEWS GVGGQHFVMFVDSGVCLDFSSGVTS*LCSLRQG PEPHSSLLWQGTVCPSQAVVRTEFSNPGAEKHW QMLLSLPPLP*AAVALRDISWQQPYPMDFYAGSS LGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP SAVACEDCSCRDEKVAPLAP*RAPGPQVSAPAL GAPRLLPLSWERLGAPRPALGAVLTFGSAPCIVL PLGERPLPVLPSLLAGHNGRAATPAGPVPGGLP AERAGLP*EDRGAEEGAAGSRRARVPQRPAPGP RPPGPRGRQIRLRVLPSGGAASRLVVFRAGGRRF LRIGRARRLQGPGGITGDGGTGTEQAGAF*RPS WFQPRGCASSPCVCPGPGRRSPLPLATVYPLGV WFLSPGHWVQGIHPAKALVIAFAPCFWGH*QSHP LSGECATGQPISRSHHPHPLWWPLHSSSCTPAS GLPQVLGPVCRVKDQEMSLLLHPAPLGAASVIH ALPGPSTHCCECASPEPVKRQLSILETQIIRALP CTEEKGSTRP*WTLTCENANMNMVGEPWIRRVT WGRQRLAPW*LPHNKVVIWILSIFFLVHTEKHF IMEVGFLAIYIVFWAAPSREVSPVVSPRTGCCMG AHTHVIGGSFKSQTEGKEA*VAQSGRNIHLRSF GKTSPGKAA*TTVI*SSSIQITESCVLVFSQTL DVLPASP*VL*PR*TMSLKGGSMPDLRWRPKTVQ NKQLLPKLTP

Frame 2

QGSPKILEVSGLRTASGGGK*LGTQLGKPVGHR FSFGCLTR*PPPCLPLPTAPGISTPSLGPFLCE AAAL*DLPQQA*PTS*GGCVSICLAMTG*RSLE AGSCPARLEGWSQQELLPWSWNFTVYRDLPGRAN WVIPALGLWGSEAMATSHLSL*DEFERWLTPAL YQVLNVVLGILSPVSCPGKLIVGRGNRRKHATI QYSKQQWVTVFTLGFIKPVNEWVCWGHCHVLQHA AWATRRPFLGSDLASLPRWVQVACPRLSIDWGT AFPKPLLTPYEVTPSSGREWALDVVLKGEVWSG VGWVGSTSLCS*IPESVSISPAESLPSCVALGKA LNHTVPFCGRGQCALHRPS*GLNSVIQGQKNTG RCYCPSLPSPRRPWL*GTFPGSSPTRWTSTLAA PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVPL RPWLARTAAAGTRRWRRWLLDALPGLRYQPPLW VRPAFCRCHGNALALRGPPSGRC*RSVPPRALC FRSGKDRFRCFRRSLPGIMAAQRPLRVLCLAGFR QSERGFREKTGALRKALRGRAELVCLSGPHPVP DPPGPEGARSDFGSCPPEEQPRGWWFSEQEADV FSALEEPAVCRGLEESLGMVAQALNRLGPFDGLL GFSQGAALAALVCALGQAGDPRFPLPRFILLVS GFCPRGIGFKESILQRPLSLPSLHVFGDTDKVI PSQESVQLASQFPGAITLTHSGGHFIPAAAPQRQ AYLKFLDQFAE*KIKKCLCSYIQLL*GQPPSSM PSQDPPLTAVSAPHQNQLRDNYQFLRPKL*GPC PVLKKRGAQGLNGH*LVKTQT*IWLESPGLGG*H GEGRGWHHGDCHIIKW*FGF*ASFSWYTQKNIL *WKSVSWPST*FSGQRQAGRCLQL*VLGQAAAW VHILTLLVEVLSPKLKGRRPRWHNQGGIYI*EVL GRHHLARLPEPQ*FSLPLSRSLRVVCWSSLKPW MCYLLLPKCSNLAEL*A*RVGRCQTLGGGQRQY RINSFFLN*P

Sequence DPH1.eAug05

CTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTT CCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTG CTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGG TGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGA GGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGGGTGCGCCCCGCCTTTTG CCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCTTCCGCTCGGGGAAAGAC CGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCGGCAGAGCGAGCGGGGCT TCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCCGACCCCCCGGGCCCCGA GGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGACACCCTTCAGCATCCCCC ACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGCCTGTCCTCCCAGGCTTC CGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCTCGATTCCCTTTTTCTCT CTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCATATCGCTGTAGGGTCCTG CCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAA TCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCC TGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCA AAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCC ATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCA AGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCA GAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGA CTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAA AGTGGTGATTTGG

DPH1.F1 (forward primer)
DPH1.R1 (reverse primer)
Intron retention

Frame 0

LWAARAVLRSWSTWNLDSEPWAFPL*GCCSLRS SPASLAYFLRWMCGCRWHVHVSPLTGAQPSPSRC *HPMRRPWL*GTFPGSSPTRWTSTLAAPWGPGR *TTARTAVPTPRAGPRGGRCRRGPRVPLRPWLA RTAAAGTRRWRRWLLDALPGLRYQPPLWVRPAFC RCHGNALALRGPPSGRC*RSVPPRALCFRSGKD RFRCFRRSLPGIMAAQRPLRVLCLAGFRQSERG FREKTGALRKALRGRAELVCLSGPHPVPDPPGPE GARSDFGETSPAPETHSVSSILFRPPRHPSASP TLRPPAFLPPSPHPSFLATHTLFAQTAACPPRL PPSPSSSAVSCSGQSTHFPPLISPRLLPRFPFSL CHLEPSCPFSFLSFLEPPMP*SHPFSSYRCRVL PSGGAASRLVVFRAGGRRFLRIGRARRLQGPGG ITGDGGTGTEQAGAF*RPSWFQPRGCASSPCVCP GPGRRSPLPLATVYPLGVWFLSPGHWVQGIHPA KALVIAFAPCFWGH*QSHPLSGECATGQPISRS HHPHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQ EMSLLLHPAPLGAASVIHALPGPSTHCCECASP EPVKRQLSILETQIIRALPCTEEKGSTRP*WTL TCENANMNMVGEPWIRRVTWGRQRLAPW*LPHNK VVIW

Frame 1

FGPPGQS*DPGAPGISTPSLGPFLCEAAAL*DL PQQA*PTS*GGCVGAGGMSTSLH*LGHSLPQAA ADTL*GGRGSEGHFLAAALPDGLLRWQLLGALDG EPRPGPPSPRPGPARAGEGAGGVRASPFGRGLR GLQLQGREGGAAGSLTRSRASGISPRSGCAPPF AVVMGTPWRSEARPRGGADVRFRPVHCASARGKT ASGASVAPCRA*WPRSDPCGSCAWRASGRASGA SVRRPGR*GRRCGVAPSSCASAARTRSPTPRAP RAPDQTSVRQAPLRKRTQFPPSCFVPLDTLQHPP PCVHPHSSHPVHTLHFSRPIPSLLKLQPVLPGF RLHRLPPLSPVQANPLISPLSFPPDFCLDSLFL SVISNPPALSHFFPSWSLPCPNPILFPHIAVGSC PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEE SLGMVAQALNRLGPFDGLLGFSQGAALAALVCA LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ RPLSLPSLHVFGDTDKVIPSQESVQLASQFPGA ITLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KI KKCLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQ NQLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH* LVKTQT*IWLESPGLGG*HGEGRGWHHGDCHII KW*F

Frame 2

LGRQGSPKILEHLESRLRALGLSFVRLLLSEIF PSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPL LTPYEAAVALRDISWQQPYPMDFYAGSSLGPWT VNHGQDRRPHAPGRPARGKVQEGSARPPSAVACE DCSCRDEKVAPLAP*RAPGPQVSAPALGAPRLL PLSWERLGAPRPALGAVLTFGSAPCIVLPLGER PLPVLPSLLAGHNGRAATPAGPVPGGLPAERAGL P*EDRGAEEGAAGSRRARVPQRPAPGPRPPGPR GRQIRLR*DKPRSGNALSFLHLVSSPSTPFSIP HPASTRIPPTQSTPFISRDPYPLCSNCSLSSQAS AFTVFLRCLLFRPIHSFPPSHFPQTFASIPFFS LSSRTLLPFLISFLPGASHALIPSFFLISL*GP ALRRSSLEAGGFQSRRPTFSPHWKSPPSAGAWRN HWGWWHRH*TGWGLLTAFLVSAKGLR*QPLCVP WARQAIPASPCHGLSSWCLVSVPGALGSRNPSC KGPCHCLRSMFLGTLTKSSPLRRVCNWPANFPEP SPSPTLVATSFQQLHPSVRPTSSSWTSLQSERS RNVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLT RTS*ETTINS*DPNYKGPALY*RKGEHKALMDID L*KRKHEYGWRALD*EGDMGKAEAGTMVTAT** SGDL

Sequence DPH1.fAug05

GCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTCAGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAG GTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCTGCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAG GCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGAGTATCTGCCTGGCTATGACTGGCTAAAGAAGCTTAGAAG CTGGGTCTTGCCCAGCTCGTCTTGAAGGCTGGAGCCAACAGGAATTGCTTCCATGGTCATGGAATTTTACTGTTTACAGAGACCTCCCTGGGAGGGCTAA CTGGGTCATACCTGCTTTGGGACTTTGGGGCTCAGAGGCAATGGCAACATCACATTTGTCCTTGTGAGATGAGTTTGAGCGGTGGCTGACACCTGCCCTG TACCAGGTACTAAATGTGGTTCTGGGGATACTATCACCAGTGAGCTGCCCTGGAAAGCTCATTGTCGGCAGAGGAAACAGGAGAAAACACGCAACAATAC AGTATTCCAAACAGCAGTGGGTCACTGTCTTTACTTTAGGATTCATAAAGCCTGTTAATGAATGGGTGTGCTGGGGGCACTGTCATGTTCTTCAGCATGC TGCCTGGGCGACGAGGAGGCCCTTCCTAGGGTCTGACCTGGCTTCCCTTCCCAGGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGTAACACCAAGCTCTGGGAGAGAGTGGGCTTTGGACGTGGTTCTCAAAGGTGAGGTCTGGAGTGGAG TGGGGTGGGTGGGCAGCACTTCGTTATGTTCGTAGATTCCGGAGTCTGTCTCGATTTCTCCAGCGGAGTCACTTCCTAGCTGTGTAGCCTTAGGCAAGGC CCTGAACCACACAGTTCCCTTCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGC AGATGTTATTGTCCCTCCCTCCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTC CTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCT TCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGG GTGCGCCCCGCCTTTTGCCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCT TCCGCTCGGGGAAAGACCGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCG GCAGAGCGAGCGGGGCTTCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCC GACCCCCCGGGCCCCGAGGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGAC ACCCTTCAGCATCCCCCACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGC CTGTCCTCCCAGGCTTCCGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCT CGATTCCCTTTTTCTCTCTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCAT ATCGCTGTAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCT GCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGC AGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTC AAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCA GCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTT TGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCT GTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAG GCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGG TGACTGCCACATAATAAAGTGGTGATTTGG

DPH1.F1 (forward primer)
DPH1.R1 (reverse primer)
Intron retention

Frame 0

ARAVLRSWRSVGSGQPLEEGSDWEHSWENQ*AT GSALAA*PGDPHPASLSQQHLESRLRALGLSFVR LLLSEIFPSKLSLLPEVDV*VSAWL*LAKEA*K LGLAQLVLKAGANRNCFHGHGILLFTETSLGGL TGSYLLWDFGAQRQWQHHICPCEMSLSGG*HLPC TRY*MWFWGYYHQ*AALESSLSAEETGENTQQY SIPNSSGSLSLL*DS*SLLMNGCAGGTVMFFSM LPGRRGGPS*GLTWLPFPGGCRWHVHVSPLTGAQ PSPSRC*HPMR*HQALGESGLWTWFSKVRSGVE WGGWAALRYVRRFRSLSRFLQRSHFLAV*P*AR P*TTQFPSVAGDSVPFTGRRED*IQ*SRGRKTLA DVIVPPSPPLGGRGSEGHFLAAALPDGLLRWQL LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP FGRGLRGLQLQGREGGAAGSLTRSRASGISPRSG CAPPFAVVMGTPWRSEARPRGGADVRFRPVHCA SARGKTASGASVAPCRA*WPRSDPCGSCAWRAS GRASGASVRRPGR*GRRCGVAPSSCASAARTRSP TPRAPRAPDQTSVRQAPLRKRTQFPPSCFVPLD TLQHPPPCVHPHSSHPVHTLHFSRPIPSLLKLQ PVLPGFRLHRLPPLSPVQANPLISPLSFPPDFCL DSLFLSVISNPPALSHFFPSWSLPCPNPILFPH IAVGSCPPEEQPRGWWFSEQEADVFSALEEPAV CRGLEESLGMVAQALNRLGPFDGLLGFSQGAALA ALVCALGQAGDPRFPLPRFILLVSGFCPRGIGF KESILQRPLSLPSLHVFGDTDKVIPSQESVQLA SQFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQF AE*KIKKCLCSYIQLL*GQPPSSMPSQDPPLTA VSAPHQNQLRDNYQFLRPKL*GPCPVLKKRGAQ GLNGH*LVKTQT*IWLESPGLGG*HGEGRGWHHG DCHIIKW*F

Frame 1

PGQS*DPGGQWAQDSLWRREVTGNTAGKTSRPQ VQLWLLDQVTPTLPPSPNSTWNLDSEPWAFPL* GCCSLRSSPASLAYFLRWMCEYLPGYDWLKKLRS WVLPSSS*RLEPTGIASMVMEFYCLQRPPWEG* LGHTCFGTLGLRGNGNITFVLVR*V*AVADTCP VPGTKCGSGDTITSELPWKAHCRQRKQEKTRNNT VFQTAVGHCLYFRIHKAC**MGVLGALSCSSAC CLGDEEALPRV*PGFPSQVGAGGMSTSLH*LGH SLPQAAADTL*GNTKLWERVGFGRGSQR*GLEWS GVGGQHFVMFVDSGVCLDFSSGVTS*LCSLRQG PEPHSSLLWQGTVCPSQAVVRTEFSNPGAEKHW QMLLSLPPLP*AAVALRDISWQQPYPMDFYAGSS LGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP SAVACEDCSCRDEKVAPLAP*RAPGPQVSAPAL GAPRLLPLSWERLGAPRPALGAVLTFGSAPCIVL PLGERPLPVLPSLLAGHNGRAATPAGPVPGGLP AERAGLP*EDRGAEEGAAGSRRARVPQRPAPGP RPPGPRGRQIRLR*DKPRSGNALSFLHLVSSPST PFSIPHPASTRIPPTQSTPFISRDPYPLCSNCS LSSQASAFTVFLRCLLFRPIHSFPPSHFPQTFA SIPFFSLSSRTLLPFLISFLPGASHALIPSFFLI SL*GPALRRSSLEAGGFQSRRPTFSPHWKSPPS AGAWRNHWGWWHRH*TGWGLLTAFLVSAKGLR* QPLCVPWARQAIPASPCHGLSSWCLVSVPGALGS RNPSCKGPCHCLRSMFLGTLTKSSPLRRVCNWP ANFPEPSPSPTLVATSFQQLHPSVRPTSSSWTS LQSERSRNVSAPTSSSSRGSLRHPCPPRTLHSLL *VRLTRTS*ETTINS*DPNYKGPALY*RKGEHK ALMDIDL*KRKHEYGWRALD*EGDMGKAEAGTM VTAT**SGDL

Frame 2

QGSPKILEVSGLRTASGGGK*LGTQLGKPVGHR FSFGCLTR*PPPCLPLPTAPGISTPSLGPFLCE AAAL*DLPQQA*PTS*GGCVSICLAMTG*RSLE AGSCPARLEGWSQQELLPWSWNFTVYRDLPGRAN WVIPALGLWGSEAMATSHLSL*DEFERWLTPAL YQVLNVVLGILSPVSCPGKLIVGRGNRRKHATI QYSKQQWVTVFTLGFIKPVNEWVCWGHCHVLQHA AWATRRPFLGSDLASLPRWVQVACPRLSIDWGT AFPKPLLTPYEVTPSSGREWALDVVLKGEVWSG VGWVGSTSLCS*IPESVSISPAESLPSCVALGKA LNHTVPFCGRGQCALHRPS*GLNSVIQGQKNTG RCYCPSLPSPRRPWL*GTFPGSSPTRWTSTLAA PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVPL RPWLARTAAAGTRRWRRWLLDALPGLRYQPPLW VRPAFCRCHGNALALRGPPSGRC*RSVPPRALC FRSGKDRFRCFRRSLPGIMAAQRPLRVLCLAGFR QSERGFREKTGALRKALRGRAELVCLSGPHPVP DPPGPEGARSDFGETSPAPETHSVSSILFRPPR HPSASPTLRPPAFLPPSPHPSFLATHTLFAQTAA CPPRLPPSPSSSAVSCSGQSTHFPPLISPRLLP RFPFSLCHLEPSCPFSFLSFLEPPMP*SHPFSS YRCRVLPSGGAASRLVVFRAGGRRFLRIGRARRL QGPGGITGDGGTGTEQAGAF*RPSWFQPRGCAS SPCVCPGPGRRSPLPLATVYPLGVWFLSPGHWV QGIHPAKALVIAFAPCFWGH*QSHPLSGECATGQ PISRSHHPHPLWWPLHSSSCTPASGLPQVLGPV CRVKDQEMSLLLHPAPLGAASVIHALPGPSTHC CECASPEPVKRQLSILETQIIRALPCTEEKGSTR P*WTLTCENANMNMVGEPWIRRVTWGRQRLAPW *LPHNKVVIW