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Sequences

Sequence DPH1.bAug05

CTGCGCTCTCCGCGTTCTTCCAGCGCTGTCTTTTTAGTACCACATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGC CGAGACGGCCCTGGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCAGCAATCCGGG TCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCT CCTCTTTGCCTGTACCATTGTGGATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGCTGTGTGGATGAC TTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTGATTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACG TCTTTGTGGACATCCGGATAGACACTACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGCAGCCGCCCAGGAGCT GAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGAGGCC GTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTAT CCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTT GGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCC AGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGA CACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAA CCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGAC TGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCA CCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAA ATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAAC CAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTG TGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTG GTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGC CAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGG GAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCT TCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGA GCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

LRSPRSSSAVFLVPHAQAGDGGAGRIRGSGAGR PRRPWQRSGPSGPRGQSDPP*DPEEPSAAGSNPG PAFQLQL*DPQDHLEDPTSPGQEGGLANAGRPP PLCLYHCGYLGKVHGGRSDGDG*RDLRGLLCG* LHSEGPGS*LLGALRPQLPDSHGHLGPRLPGAVR LCGHPDRHYTPPGLSPPHLSPSHCPCPGSRPGA ESRVSCECPTVQAPVPWRDPGLHIPPTVQRGGG RCVSWRWPLPSGVCHDCQPQCPRLPV*PI*QSPI QRTL*PPAHAGCSPRSHSHCPLS*VLGPYSGHF GPPGQS*DPGAPGISTPSLGPFLCEAAAL*DLP QQA*PTS*GGCVGAGGMSTSLH*LGHSLPQAAAD TL*GGRGSEGHFLAAALPDGLLRWQLLGALDGE PRPGPPSPRPGPARAGEGAGGVRASPFGRGLRG LQLQGREGGAAGSLTRSRASGECATGQPISRSHH PHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQE MSLLLHPAPLGAASVIHALPGPSTHCCECASPE PVKRQLSILETQIIRALPCTEEKGSTRP*WTLTC ENANMNMVGEPWIRRVTWGRQRLAPW*LPHNKV VIWILSIFFLVHTEKHFIMEVGFLAIYIVFWAA PSREVSPVVSPRTGCCMGAHTHVIGGSFKSQTEG KEA*VAQSGRNIHLRSFGKTSPGKAA*TTVI*S SSIQITESCVLVFSQTLDVLPASP*VL*PR*TM SLKGGSMPDLRWRPKTVQNKQLLPKLTP

Frame 1

CALRVLPALSF*YHMRRQVMAALVVSGAAEQGG RDGPGRGRAPRGRVANQIPPEILKNPQLQAAIR VLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLL LFACTIVDILERFTEAEVMVMGDVTYGACCVDD FTARALGADFLVHYGHSCLIPMDTSAQDFRVLY VFVDIRIDTTHLLDSLRLTFPPATALALAAAQEL KAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEA VVYLGDGRFHLESVMIANPNVPAYRYDPYSKVL SREHYDHQRMQAARQEAIATARSAKSWGLILGTL GRQGSPKILEHLESRLRALGLSFVRLLLSEIFP SKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLL TPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVN HGQDRRPHAPGRPARGKVQEGSARPPSAVACED CSCRDEKVAPLAP*RAPGPQESVQLASQFPGAI TLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KIKK CLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQN QLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH*L VKTQT*IWLESPGLGG*HGEGRGWHHGDCHIIKW *FGF*ASFSWYTQKNIL*WKSVSWPST*FSGQR QAGRCLQL*VLGQAAAWVHILTLLVEVLSPKLK GRRPRWHNQGGIYI*EVLGRHHLARLPEPQ*FSL PLSRSLRVVCWSSLKPWMCYLLLPKCSNLAEL* A*RVGRCQTLGGGQRQYRINSFFLN*P

Frame 2

ALSAFFQRCLFSTTCAGR*WRRWSYPGQRSRAA ETALAEVGPLGAAWPIRSPLRS*RTLSCRQQSG SCLPTTTLRSPRPSGGSNKPRPRRWPCKCRKAS SSLPVPLWISWKGSRRPK*W*WVT*PTGLAVWMT SQRGPWELTSWCTTATVA*FPWTPRPKTSGCCT SLWTSG*TLHTSWTLSASPFPQPLPLPWQPPRS *KPSIV*VSHSASPCPLERSWAAHPPDCPKRWRP LCILEMAASIWSLS*LPTPMSPLTGMTHIAKSY PENTMTTSACRLLAKKP*PLPAQLSPGALFWAL WAARAVLRSWSTWNLDSEPWAFPL*GCCSLRSSP ASLAYFLRWMCGCRWHVHVSPLTGAQPSPSRC* HPMRRPWL*GTFPGSSPTRWTSTLAAPWGPGR* TTARTAVPTPRAGPRGGRCRRGPRVPLRPWLART AAAGTRRWRRWLLDALPGLRRVCNWPANFPEPS PSPTLVATSFQQLHPSVRPTSSSWTSLQSERSR NVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLTRT S*ETTINS*DPNYKGPALY*RKGEHKALMDIDL *KRKHEYGWRALD*EGDMGKAEAGTMVTAT**S GDLDFEHLFPGTHRKTFYNGSRFLGHLHSFLGSA KQGGVSSCESSDRLLHGCTYSRYWWKF*VPN*R EGGLGGTIREEYTSEKFWEDITWQGCLNHSNLV FLYPDH*ELCAGLLSNPGCVTCFSLSALTSLNYE PEGWVDARP*VEAKDSTE*TASS*IDP

Sequence DPH1.dAug05

CTGCGCTCTCCGCGTTCTTCCAGCGCTGTCTTTTTAGTACCACATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGC CGAGACGGCCCTGGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCAGCAATCCGGG TCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCT CCTCTTTGCCTGTACCATTGTGGATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGCTGTGTGGATGAC TTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTGATTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACG TCTTTGTGGACATCCGGATAGACACTACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTT TGTGTCGACCTTGCAGGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGC ACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCG CTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTC AGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCC TTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCA GCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGG GGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCG CTCCCGGGCCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCA GCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCA TCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGC CCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGG GTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAAC ATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTG CATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGA AGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAA CCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGAC AGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

LRSPRSSSAVFLVPHAQAGDGGAGRIRGSGAGR PRRPWQRSGPSGPRGQSDPP*DPEEPSAAGSNPG PAFQLQL*DPQDHLEDPTSPGQEGGLANAGRPP PLCLYHCGYLGKVHGGRSDGDG*RDLRGLLCG* LHSEGPGS*LLGALRPQLPDSHGHLGPRLPGAVR LCGHPDRHYTPPGLSPPHLSPSHCPCPGQHHSV CVDLAGSRPGAESRVSCECPTVQAPVPWRDPGL HIPPTVQRGGGRCVSWRWPLPSGVCHDCQPQCPR LPV*PI*QSPIQRTL*PPAHAGCSPRSHSHCPL S*VLGPYSGHFGPPGQS*DPGAPGISTPSLGPF LCEAAAL*DLPQQA*PTS*GGCAAVALRDISWQQ PYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPAR GKVQEGSARPPSAVACEDCSCRDEKVAPLAP*R APGPQESVQLASQFPGAITLTHSGGHFIPAAAPQ RQAYLKFLDQFAE*KIKKCLCSYIQLL*GQPPS SMPSQDPPLTAVSAPHQNQLRDNYQFLRPKL*G PCPVLKKRGAQGLNGH*LVKTQT*IWLESPGLGG *HGEGRGWHHGDCHIIKW*FGF*ASFSWYTQKN IL*WKSVSWPST*FSGQRQAGRCLQL*VLGQAA AWVHILTLLVEVLSPKLKGRRPRWHNQGGIYI*E VLGRHHLARLPEPQ*FSLPLSRSLRVVCWSSLK PWMCYLLLPKCSNLAEL*A*RVGRCQTLGGGQR QYRINSFFLN*P

Frame 1

CALRVLPALSF*YHMRRQVMAALVVSGAAEQGG RDGPGRGRAPRGRVANQIPPEILKNPQLQAAIR VLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLL LFACTIVDILERFTEAEVMVMGDVTYGACCVDD FTARALGADFLVHYGHSCLIPMDTSAQDFRVLY VFVDIRIDTTHLLDSLRLTFPPATALALVSTIQF VSTLQAAAQELKAEYRVSVPQCKPLSPGEILGC TSPRLSKEVEAVVYLGDGRFHLESVMIANPNVP AYRYDPYSKVLSREHYDHQRMQAARQEAIATARS AKSWGLILGTLGRQGSPKILEHLESRLRALGLS FVRLLLSEIFPSKLSLLPEVDVRPWL*GTFPGS SPTRWTSTLAAPWGPGR*TTARTAVPTPRAGPRG GRCRRGPRVPLRPWLARTAAAGTRRWRRWLLDA LPGLRRVCNWPANFPEPSPSPTLVATSFQQLHP SVRPTSSSWTSLQSERSRNVSAPTSSSSRGSLRH PCPPRTLHSLL*VRLTRTS*ETTINS*DPNYKG PALY*RKGEHKALMDIDL*KRKHEYGWRALD*E GDMGKAEAGTMVTAT**SGDLDFEHLFPGTHRKT FYNGSRFLGHLHSFLGSAKQGGVSSCESSDRLL HGCTYSRYWWKF*VPN*REGGLGGTIREEYTSE KFWEDITWQGCLNHSNLVFLYPDH*ELCAGLLSN PGCVTCFSLSALTSLNYEPEGWVDARP*VEAKD STE*TASS*IDP

Frame 2

ALSAFFQRCLFSTTCAGR*WRRWSYPGQRSRAA ETALAEVGPLGAAWPIRSPLRS*RTLSCRQQSG SCLPTTTLRSPRPSGGSNKPRPRRWPCKCRKAS SSLPVPLWISWKGSRRPK*W*WVT*PTGLAVWMT SQRGPWELTSWCTTATVA*FPWTPRPKTSGCCT SLWTSG*TLHTSWTLSASPFPQPLPLPWSAPFS LCRPCRQPPRS*KPSIV*VSHSASPCPLERSWAA HPPDCPKRWRPLCILEMAASIWSLS*LPTPMSP LTGMTHIAKSYPENTMTTSACRLLAKKP*PLPA QLSPGALFWALWAARAVLRSWSTWNLDSEPWAFP L*GCCSLRSSPASLAYFLRWMCGRGSEGHFLAA ALPDGLLRWQLLGALDGEPRPGPPSPRPGPARA GEGAGGVRASPFGRGLRGLQLQGREGGAAGSLTR SRASGECATGQPISRSHHPHPLWWPLHSSSCTP ASGLPQVLGPVCRVKDQEMSLLLHPAPLGAASV IHALPGPSTHCCECASPEPVKRQLSILETQIIRA LPCTEEKGSTRP*WTLTCENANMNMVGEPWIRR VTWGRQRLAPW*LPHNKVVIWILSIFFLVHTEK HFIMEVGFLAIYIVFWAAPSREVSPVVSPRTGCC MGAHTHVIGGSFKSQTEGKEA*VAQSGRNIHLR SFGKTSPGKAA*TTVI*SSSIQITESCVLVFSQ TLDVLPASP*VL*PR*TMSLKGGSMPDLRWRPKT VQNKQLLPKLTP

Sequence DPH1.eAug05

CTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTT CCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTG CTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGG TGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGA GGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGGGTGCGCCCCGCCTTTTG CCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCTTCCGCTCGGGGAAAGAC CGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCGGCAGAGCGAGCGGGGCT TCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCCGACCCCCCGGGCCCCGA GGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGACACCCTTCAGCATCCCCC ACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGCCTGTCCTCCCAGGCTTC CGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCTCGATTCCCTTTTTCTCT CTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCATATCGCTGTAGGGTCCTG CCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAA TCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCC TGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCA AAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCC ATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCA AGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCA GAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGA CTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAA AGTGGTGATTTGG

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

LWAARAVLRSWSTWNLDSEPWAFPL*GCCSLRS SPASLAYFLRWMCGCRWHVHVSPLTGAQPSPSRC *HPMRRPWL*GTFPGSSPTRWTSTLAAPWGPGR *TTARTAVPTPRAGPRGGRCRRGPRVPLRPWLA RTAAAGTRRWRRWLLDALPGLRYQPPLWVRPAFC RCHGNALALRGPPSGRC*RSVPPRALCFRSGKD RFRCFRRSLPGIMAAQRPLRVLCLAGFRQSERG FREKTGALRKALRGRAELVCLSGPHPVPDPPGPE GARSDFGETSPAPETHSVSSILFRPPRHPSASP TLRPPAFLPPSPHPSFLATHTLFAQTAACPPRL PPSPSSSAVSCSGQSTHFPPLISPRLLPRFPFSL CHLEPSCPFSFLSFLEPPMP*SHPFSSYRCRVL PSGGAASRLVVFRAGGRRFLRIGRARRLQGPGG ITGDGGTGTEQAGAF*RPSWFQPRGCASSPCVCP GPGRRSPLPLATVYPLGVWFLSPGHWVQGIHPA KALVIAFAPCFWGH*QSHPLSGECATGQPISRS HHPHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQ EMSLLLHPAPLGAASVIHALPGPSTHCCECASP EPVKRQLSILETQIIRALPCTEEKGSTRP*WTL TCENANMNMVGEPWIRRVTWGRQRLAPW*LPHNK VVIW

Frame 1

FGPPGQS*DPGAPGISTPSLGPFLCEAAAL*DL PQQA*PTS*GGCVGAGGMSTSLH*LGHSLPQAA ADTL*GGRGSEGHFLAAALPDGLLRWQLLGALDG EPRPGPPSPRPGPARAGEGAGGVRASPFGRGLR GLQLQGREGGAAGSLTRSRASGISPRSGCAPPF AVVMGTPWRSEARPRGGADVRFRPVHCASARGKT ASGASVAPCRA*WPRSDPCGSCAWRASGRASGA SVRRPGR*GRRCGVAPSSCASAARTRSPTPRAP RAPDQTSVRQAPLRKRTQFPPSCFVPLDTLQHPP PCVHPHSSHPVHTLHFSRPIPSLLKLQPVLPGF RLHRLPPLSPVQANPLISPLSFPPDFCLDSLFL SVISNPPALSHFFPSWSLPCPNPILFPHIAVGSC PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEE SLGMVAQALNRLGPFDGLLGFSQGAALAALVCA LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ RPLSLPSLHVFGDTDKVIPSQESVQLASQFPGA ITLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KI KKCLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQ NQLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH* LVKTQT*IWLESPGLGG*HGEGRGWHHGDCHII KW*F

Frame 2

LGRQGSPKILEHLESRLRALGLSFVRLLLSEIF PSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPL LTPYEAAVALRDISWQQPYPMDFYAGSSLGPWT VNHGQDRRPHAPGRPARGKVQEGSARPPSAVACE DCSCRDEKVAPLAP*RAPGPQVSAPALGAPRLL PLSWERLGAPRPALGAVLTFGSAPCIVLPLGER PLPVLPSLLAGHNGRAATPAGPVPGGLPAERAGL P*EDRGAEEGAAGSRRARVPQRPAPGPRPPGPR GRQIRLR*DKPRSGNALSFLHLVSSPSTPFSIP HPASTRIPPTQSTPFISRDPYPLCSNCSLSSQAS AFTVFLRCLLFRPIHSFPPSHFPQTFASIPFFS LSSRTLLPFLISFLPGASHALIPSFFLISL*GP ALRRSSLEAGGFQSRRPTFSPHWKSPPSAGAWRN HWGWWHRH*TGWGLLTAFLVSAKGLR*QPLCVP WARQAIPASPCHGLSSWCLVSVPGALGSRNPSC KGPCHCLRSMFLGTLTKSSPLRRVCNWPANFPEP SPSPTLVATSFQQLHPSVRPTSSSWTSLQSERS RNVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLT RTS*ETTINS*DPNYKGPALY*RKGEHKALMDID L*KRKHEYGWRALD*EGDMGKAEAGTMVTAT** SGDL

Sequence DPH1.fAug05

GCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTCAGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAG GTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCTGCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAG GCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGAGTATCTGCCTGGCTATGACTGGCTAAAGAAGCTTAGAAG CTGGGTCTTGCCCAGCTCGTCTTGAAGGCTGGAGCCAACAGGAATTGCTTCCATGGTCATGGAATTTTACTGTTTACAGAGACCTCCCTGGGAGGGCTAA CTGGGTCATACCTGCTTTGGGACTTTGGGGCTCAGAGGCAATGGCAACATCACATTTGTCCTTGTGAGATGAGTTTGAGCGGTGGCTGACACCTGCCCTG TACCAGGTACTAAATGTGGTTCTGGGGATACTATCACCAGTGAGCTGCCCTGGAAAGCTCATTGTCGGCAGAGGAAACAGGAGAAAACACGCAACAATAC AGTATTCCAAACAGCAGTGGGTCACTGTCTTTACTTTAGGATTCATAAAGCCTGTTAATGAATGGGTGTGCTGGGGGCACTGTCATGTTCTTCAGCATGC TGCCTGGGCGACGAGGAGGCCCTTCCTAGGGTCTGACCTGGCTTCCCTTCCCAGGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGTAACACCAAGCTCTGGGAGAGAGTGGGCTTTGGACGTGGTTCTCAAAGGTGAGGTCTGGAGTGGAG TGGGGTGGGTGGGCAGCACTTCGTTATGTTCGTAGATTCCGGAGTCTGTCTCGATTTCTCCAGCGGAGTCACTTCCTAGCTGTGTAGCCTTAGGCAAGGC CCTGAACCACACAGTTCCCTTCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGC AGATGTTATTGTCCCTCCCTCCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTC CTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCT TCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGG GTGCGCCCCGCCTTTTGCCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCT TCCGCTCGGGGAAAGACCGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCG GCAGAGCGAGCGGGGCTTCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCC GACCCCCCGGGCCCCGAGGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGAC ACCCTTCAGCATCCCCCACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGC CTGTCCTCCCAGGCTTCCGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCT CGATTCCCTTTTTCTCTCTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCAT ATCGCTGTAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCT GCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGC AGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTC AAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCA GCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTT TGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCT GTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAG GCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGG TGACTGCCACATAATAAAGTGGTGATTTGG

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

ARAVLRSWRSVGSGQPLEEGSDWEHSWENQ*AT GSALAA*PGDPHPASLSQQHLESRLRALGLSFVR LLLSEIFPSKLSLLPEVDV*VSAWL*LAKEA*K LGLAQLVLKAGANRNCFHGHGILLFTETSLGGL TGSYLLWDFGAQRQWQHHICPCEMSLSGG*HLPC TRY*MWFWGYYHQ*AALESSLSAEETGENTQQY SIPNSSGSLSLL*DS*SLLMNGCAGGTVMFFSM LPGRRGGPS*GLTWLPFPGGCRWHVHVSPLTGAQ PSPSRC*HPMR*HQALGESGLWTWFSKVRSGVE WGGWAALRYVRRFRSLSRFLQRSHFLAV*P*AR P*TTQFPSVAGDSVPFTGRRED*IQ*SRGRKTLA DVIVPPSPPLGGRGSEGHFLAAALPDGLLRWQL LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP FGRGLRGLQLQGREGGAAGSLTRSRASGISPRSG CAPPFAVVMGTPWRSEARPRGGADVRFRPVHCA SARGKTASGASVAPCRA*WPRSDPCGSCAWRAS GRASGASVRRPGR*GRRCGVAPSSCASAARTRSP TPRAPRAPDQTSVRQAPLRKRTQFPPSCFVPLD TLQHPPPCVHPHSSHPVHTLHFSRPIPSLLKLQ PVLPGFRLHRLPPLSPVQANPLISPLSFPPDFCL DSLFLSVISNPPALSHFFPSWSLPCPNPILFPH IAVGSCPPEEQPRGWWFSEQEADVFSALEEPAV CRGLEESLGMVAQALNRLGPFDGLLGFSQGAALA ALVCALGQAGDPRFPLPRFILLVSGFCPRGIGF KESILQRPLSLPSLHVFGDTDKVIPSQESVQLA SQFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQF AE*KIKKCLCSYIQLL*GQPPSSMPSQDPPLTA VSAPHQNQLRDNYQFLRPKL*GPCPVLKKRGAQ GLNGH*LVKTQT*IWLESPGLGG*HGEGRGWHHG DCHIIKW*F

Frame 1

PGQS*DPGGQWAQDSLWRREVTGNTAGKTSRPQ VQLWLLDQVTPTLPPSPNSTWNLDSEPWAFPL* GCCSLRSSPASLAYFLRWMCEYLPGYDWLKKLRS WVLPSSS*RLEPTGIASMVMEFYCLQRPPWEG* LGHTCFGTLGLRGNGNITFVLVR*V*AVADTCP VPGTKCGSGDTITSELPWKAHCRQRKQEKTRNNT VFQTAVGHCLYFRIHKAC**MGVLGALSCSSAC CLGDEEALPRV*PGFPSQVGAGGMSTSLH*LGH SLPQAAADTL*GNTKLWERVGFGRGSQR*GLEWS GVGGQHFVMFVDSGVCLDFSSGVTS*LCSLRQG PEPHSSLLWQGTVCPSQAVVRTEFSNPGAEKHW QMLLSLPPLP*AAVALRDISWQQPYPMDFYAGSS LGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP SAVACEDCSCRDEKVAPLAP*RAPGPQVSAPAL GAPRLLPLSWERLGAPRPALGAVLTFGSAPCIVL PLGERPLPVLPSLLAGHNGRAATPAGPVPGGLP AERAGLP*EDRGAEEGAAGSRRARVPQRPAPGP RPPGPRGRQIRLR*DKPRSGNALSFLHLVSSPST PFSIPHPASTRIPPTQSTPFISRDPYPLCSNCS LSSQASAFTVFLRCLLFRPIHSFPPSHFPQTFA SIPFFSLSSRTLLPFLISFLPGASHALIPSFFLI SL*GPALRRSSLEAGGFQSRRPTFSPHWKSPPS AGAWRNHWGWWHRH*TGWGLLTAFLVSAKGLR* QPLCVPWARQAIPASPCHGLSSWCLVSVPGALGS RNPSCKGPCHCLRSMFLGTLTKSSPLRRVCNWP ANFPEPSPSPTLVATSFQQLHPSVRPTSSSWTS LQSERSRNVSAPTSSSSRGSLRHPCPPRTLHSLL *VRLTRTS*ETTINS*DPNYKGPALY*RKGEHK ALMDIDL*KRKHEYGWRALD*EGDMGKAEAGTM VTAT**SGDL

Frame 2

QGSPKILEVSGLRTASGGGK*LGTQLGKPVGHR FSFGCLTR*PPPCLPLPTAPGISTPSLGPFLCE AAAL*DLPQQA*PTS*GGCVSICLAMTG*RSLE AGSCPARLEGWSQQELLPWSWNFTVYRDLPGRAN WVIPALGLWGSEAMATSHLSL*DEFERWLTPAL YQVLNVVLGILSPVSCPGKLIVGRGNRRKHATI QYSKQQWVTVFTLGFIKPVNEWVCWGHCHVLQHA AWATRRPFLGSDLASLPRWVQVACPRLSIDWGT AFPKPLLTPYEVTPSSGREWALDVVLKGEVWSG VGWVGSTSLCS*IPESVSISPAESLPSCVALGKA LNHTVPFCGRGQCALHRPS*GLNSVIQGQKNTG RCYCPSLPSPRRPWL*GTFPGSSPTRWTSTLAA PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVPL RPWLARTAAAGTRRWRRWLLDALPGLRYQPPLW VRPAFCRCHGNALALRGPPSGRC*RSVPPRALC FRSGKDRFRCFRRSLPGIMAAQRPLRVLCLAGFR QSERGFREKTGALRKALRGRAELVCLSGPHPVP DPPGPEGARSDFGETSPAPETHSVSSILFRPPR HPSASPTLRPPAFLPPSPHPSFLATHTLFAQTAA CPPRLPPSPSSSAVSCSGQSTHFPPLISPRLLP RFPFSLCHLEPSCPFSFLSFLEPPMP*SHPFSS YRCRVLPSGGAASRLVVFRAGGRRFLRIGRARRL QGPGGITGDGGTGTEQAGAF*RPSWFQPRGCAS SPCVCPGPGRRSPLPLATVYPLGVWFLSPGHWV QGIHPAKALVIAFAPCFWGH*QSHPLSGECATGQ PISRSHHPHPLWWPLHSSSCTPASGLPQVLGPV CRVKDQEMSLLLHPAPLGAASVIHALPGPSTHC CECASPEPVKRQLSILETQIIRALPCTEEKGSTR P*WTLTCENANMNMVGEPWIRRVTWGRQRLAPW *LPHNKVVIW