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Sequences

Sequence DPH1.aAug05

CTGCGCTCTCCGCGTTCTTCCAGCGCTGTCTTTTTAGTACCACATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGC CGAGACGGCCCTGGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCAGCAATCCGGG TCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCT CCTCTTTGCCTGTACCATTGTGGATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGCTGTGTGGATGAC TTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTGATTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACG TCTTTGTGGACATCCGGATAGACACTACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTT TGTGTCGACCTTGCAGGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGC ACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCG CTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTC AGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCC TTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTG ACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTA CGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCC GCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTG CCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAA TCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCC TGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCA AAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCC ATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCA AGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCA GAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGA CTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAA AGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCA GCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTG AAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTA GTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACT ATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

LRSPRSSSAVFLVPHAQAGDGGAGRIRGSGAGR PRRPWQRSGPSGPRGQSDPP*DPEEPSAAGSNPG PAFQLQL*DPQDHLEDPTSPGQEGGLANAGRPP PLCLYHCGYLGKVHGGRSDGDG*RDLRGLLCG* LHSEGPGS*LLGALRPQLPDSHGHLGPRLPGAVR LCGHPDRHYTPPGLSPPHLSPSHCPCPGQHHSV CVDLAGSRPGAESRVSCECPTVQAPVPWRDPGL HIPPTVQRGGGRCVSWRWPLPSGVCHDCQPQCPR LPV*PI*QSPIQRTL*PPAHAGCSPRSHSHCPL S*VLGPYSGHFGPPGQS*DPGAPGISTPSLGPF LCEAAAL*DLPQQA*PTS*GGCVGAGGMSTSLH* LGHSLPQAAADTL*GGRGSEGHFLAAALPDGLL RWQLLGALDGEPRPGPPSPRPGPARAGEGAGGV RASPFGRGLRGLQLQGREGGAAGSLTRSRASGSC PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEE SLGMVAQALNRLGPFDGLLGFSQGAALAALVCA LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ RPLSLPSLHVFGDTDKVIPSQESVQLASQFPGA ITLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KI KKCLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQ NQLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH* LVKTQT*IWLESPGLGG*HGEGRGWHHGDCHII KW*FGF*ASFSWYTQKNIL*WKSVSWPST*FSGQ RQAGRCLQL*VLGQAAAWVHILTLLVEVLSPKL KGRRPRWHNQGGIYI*EVLGRHHLARLPEPQ*F SLPLSRSLRVVCWSSLKPWMCYLLLPKCSNLAEL *A*RVGRCQTLGGGQRQYRINSFFLN*P

Frame 1

CALRVLPALSF*YHMRRQVMAALVVSGAAEQGG RDGPGRGRAPRGRVANQIPPEILKNPQLQAAIR VLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLL LFACTIVDILERFTEAEVMVMGDVTYGACCVDD FTARALGADFLVHYGHSCLIPMDTSAQDFRVLY VFVDIRIDTTHLLDSLRLTFPPATALALVSTIQF VSTLQAAAQELKAEYRVSVPQCKPLSPGEILGC TSPRLSKEVEAVVYLGDGRFHLESVMIANPNVP AYRYDPYSKVLSREHYDHQRMQAARQEAIATARS AKSWGLILGTLGRQGSPKILEHLESRLRALGLS FVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSI DWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFY AGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGS ARPPSAVACEDCSCRDEKVAPLAP*RAPGPQGP ALRRSSLEAGGFQSRRPTFSPHWKSPPSAGAWRN HWGWWHRH*TGWGLLTAFLVSAKGLR*QPLCVP WARQAIPASPCHGLSSWCLVSVPGALGSRNPSC KGPCHCLRSMFLGTLTKSSPLRRVCNWPANFPEP SPSPTLVATSFQQLHPSVRPTSSSWTSLQSERS RNVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLT RTS*ETTINS*DPNYKGPALY*RKGEHKALMDID L*KRKHEYGWRALD*EGDMGKAEAGTMVTAT** SGDLDFEHLFPGTHRKTFYNGSRFLGHLHSFLG SAKQGGVSSCESSDRLLHGCTYSRYWWKF*VPN* REGGLGGTIREEYTSEKFWEDITWQGCLNHSNL VFLYPDH*ELCAGLLSNPGCVTCFSLSALTSLN YEPEGWVDARP*VEAKDSTE*TASS*IDP

Frame 2

ALSAFFQRCLFSTTCAGR*WRRWSYPGQRSRAA ETALAEVGPLGAAWPIRSPLRS*RTLSCRQQSG SCLPTTTLRSPRPSGGSNKPRPRRWPCKCRKAS SSLPVPLWISWKGSRRPK*W*WVT*PTGLAVWMT SQRGPWELTSWCTTATVA*FPWTPRPKTSGCCT SLWTSG*TLHTSWTLSASPFPQPLPLPWSAPFS LCRPCRQPPRS*KPSIV*VSHSASPCPLERSWAA HPPDCPKRWRPLCILEMAASIWSLS*LPTPMSP LTGMTHIAKSYPENTMTTSACRLLAKKP*PLPA QLSPGALFWALWAARAVLRSWSTWNLDSEPWAFP L*GCCSLRSSPASLAYFLRWMCGCRWHVHVSPL TGAQPSPSRC*HPMRRPWL*GTFPGSSPTRWTS TLAAPWGPGR*TTARTAVPTPRAGPRGGRCRRGP RVPLRPWLARTAAAGTRRWRRWLLDALPGLRVL PSGGAASRLVVFRAGGRRFLRIGRARRLQGPGG ITGDGGTGTEQAGAF*RPSWFQPRGCASSPCVCP GPGRRSPLPLATVYPLGVWFLSPGHWVQGIHPA KALVIAFAPCFWGH*QSHPLSGECATGQPISRS HHPHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQ EMSLLLHPAPLGAASVIHALPGPSTHCCECASP EPVKRQLSILETQIIRALPCTEEKGSTRP*WTL TCENANMNMVGEPWIRRVTWGRQRLAPW*LPHNK VVIWILSIFFLVHTEKHFIMEVGFLAIYIVFWA APSREVSPVVSPRTGCCMGAHTHVIGGSFKSQT EGKEA*VAQSGRNIHLRSFGKTSPGKAA*TTVI* SSSIQITESCVLVFSQTLDVLPASP*VL*PR*T MSLKGGSMPDLRWRPKTVQNKQLLPKLTP

Sequence DPH1.cAug05

CTGCGCTCTCCGCGTTCTTCCAGCGCTGTCTTTTTAGTACCACATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGC CGAGACGGCCCTGGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCAGCAATCCGGG TCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCT CCTCTTTGCCTGTACCATTGTGGATATCTTGGAAAGTTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACGTCTTTGTGGACATCCGGATAGA CACTACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTTTGTGTCGACCTTGCAGGCAGCC GCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAG AGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAG CAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATT CTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTG AGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAA GCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCC TGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGG CTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGA GGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGG CACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCG CTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCG CTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTG GCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACAT CCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTAT CAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAA TATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGC ATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTC CAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGC ACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACT GAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGA TGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

LRSPRSSSAVFLVPHAQAGDGGAGRIRGSGAGR PRRPWQRSGPSGPRGQSDPP*DPEEPSAAGSNPG PAFQLQL*DPQDHLEDPTSPGQEGGLANAGRPP PLCLYHCGYLGKFPWTPRPKTSGCCTSLWTSG* TLHTSWTLSASPFPQPLPLPWSAPFSLCRPCRQP PRS*KPSIV*VSHSASPCPLERSWAAHPPDCPK RWRPLCILEMAASIWSLS*LPTPMSPLTGMTHI AKSYPENTMTTSACRLLAKKP*PLPAQLSPGALF WALWAARAVLRSWSTWNLDSEPWAFPL*GCCSL RSSPASLAYFLRWMCGCRWHVHVSPLTGAQPSP SRC*HPMRRPWL*GTFPGSSPTRWTSTLAAPWGP GR*TTARTAVPTPRAGPRGGRCRRGPRVPLRPW LARTAAAGTRRWRRWLLDALPGLRVLPSGGAAS RLVVFRAGGRRFLRIGRARRLQGPGGITGDGGTG TEQAGAF*RPSWFQPRGCASSPCVCPGPGRRSP LPLATVYPLGVWFLSPGHWVQGIHPAKALVIAF APCFWGH*QSHPLSGECATGQPISRSHHPHPLWW PLHSSSCTPASGLPQVLGPVCRVKDQEMSLLLH PAPLGAASVIHALPGPSTHCCECASPEPVKRQL SILETQIIRALPCTEEKGSTRP*WTLTCENANMN MVGEPWIRRVTWGRQRLAPW*LPHNKVVIWILS IFFLVHTEKHFIMEVGFLAIYIVFWAAPSREVS PVVSPRTGCCMGAHTHVIGGSFKSQTEGKEA*VA QSGRNIHLRSFGKTSPGKAA*TTVI*SSSIQIT ESCVLVFSQTLDVLPASP*VL*PR*TMSLKGGS MPDLRWRPKTVQNKQLLPKLTP

Frame 1

CALRVLPALSF*YHMRRQVMAALVVSGAAEQGG RDGPGRGRAPRGRVANQIPPEILKNPQLQAAIR VLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLL LFACTIVDILESSHGHLGPRLPGAVRLCGHPDR HYTPPGLSPPHLSPSHCPCPGQHHSVCVDLAGS RPGAESRVSCECPTVQAPVPWRDPGLHIPPTVQR GGGRCVSWRWPLPSGVCHDCQPQCPRLPV*PI* QSPIQRTL*PPAHAGCSPRSHSHCPLS*VLGPY SGHFGPPGQS*DPGAPGISTPSLGPFLCEAAAL* DLPQQA*PTS*GGCVGAGGMSTSLH*LGHSLPQ AAADTL*GGRGSEGHFLAAALPDGLLRWQLLGA LDGEPRPGPPSPRPGPARAGEGAGGVRASPFGRG LRGLQLQGREGGAAGSLTRSRASGSCPPEEQPR GWWFSEQEADVFSALEEPAVCRGLEESLGMVAQ ALNRLGPFDGLLGFSQGAALAALVCALGQAGDPR FPLPRFILLVSGFCPRGIGFKESILQRPLSLPS LHVFGDTDKVIPSQESVQLASQFPGAITLTHSG GHFIPAAAPQRQAYLKFLDQFAE*KIKKCLCSYI QLL*GQPPSSMPSQDPPLTAVSAPHQNQLRDNY QFLRPKL*GPCPVLKKRGAQGLNGH*LVKTQT* IWLESPGLGG*HGEGRGWHHGDCHIIKW*FGF*A SFSWYTQKNIL*WKSVSWPST*FSGQRQAGRCL QL*VLGQAAAWVHILTLLVEVLSPKLKGRRPRW HNQGGIYI*EVLGRHHLARLPEPQ*FSLPLSRSL RVVCWSSLKPWMCYLLLPKCSNLAEL*A*RVGR CQTLGGGQRQYRINSFFLN*P

Frame 2

ALSAFFQRCLFSTTCAGR*WRRWSYPGQRSRAA ETALAEVGPLGAAWPIRSPLRS*RTLSCRQQSG SCLPTTTLRSPRPSGGSNKPRPRRWPCKCRKAS SSLPVPLWISWKVPMDTSAQDFRVLYVFVDIRID TTHLLDSLRLTFPPATALALVSTIQFVSTLQAA AQELKAEYRVSVPQCKPLSPGEILGCTSPRLSK EVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYS KVLSREHYDHQRMQAARQEAIATARSAKSWGLI LGTLGRQGSPKILEHLESRLRALGLSFVRLLLS EIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPK PLLTPYEAAVALRDISWQQPYPMDFYAGSSLGP WTVNHGQDRRPHAPGRPARGKVQEGSARPPSAV ACEDCSCRDEKVAPLAP*RAPGPQGPALRRSSLE AGGFQSRRPTFSPHWKSPPSAGAWRNHWGWWHR H*TGWGLLTAFLVSAKGLR*QPLCVPWARQAIP ASPCHGLSSWCLVSVPGALGSRNPSCKGPCHCLR SMFLGTLTKSSPLRRVCNWPANFPEPSPSPTLV ATSFQQLHPSVRPTSSSWTSLQSERSRNVSAPT SSSSRGSLRHPCPPRTLHSLL*VRLTRTS*ETTI NS*DPNYKGPALY*RKGEHKALMDIDL*KRKHE YGWRALD*EGDMGKAEAGTMVTAT**SGDLDFE HLFPGTHRKTFYNGSRFLGHLHSFLGSAKQGGVS SCESSDRLLHGCTYSRYWWKF*VPN*REGGLGG TIREEYTSEKFWEDITWQGCLNHSNLVFLYPDH *ELCAGLLSNPGCVTCFSLSALTSLNYEPEGWVD ARP*VEAKDSTE*TASS*IDP

Sequence DPH1.hAug05

TTCACACGTGAGAAAACCAAGGCTCCAAGAGGTGAAGTGAAGGCTTGTCCCAGTTTACACAGAGGCACAGCACAGCCAGCTGTGAACTCAGTCCTCTGGA CTTTTTTTTTTTTTTGAGACAGAGTCTTGTTCTGTCGCCCAGGCTGGAGTGCAGAGGTGCGATCTCGGCTCACTGCAGCCTCTGCCTCCCGGGTTCAAGT GATTATCCTGCTTCAGCCTCCCCAGTAGCTGGGACTACAGGTGCCCGCCACCACGCCCAGCTAAGTTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGT GTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACTGCACCTGGCAGCCC TGTGGACTTCTTTTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAAGCTACG CCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGTCACCACACCCAGCTAAGTTTTTGTATTTTTAGTAGA GACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCCGCCCGCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA CCGCGCCCGGCCAGCCCTGTGGACTTCTAAGCCAGCGAGTGCCTCTTCTGCTGCCCTAAACCACACTTAGCCTCCTTTCCACCCCTGCAGGTATCTTGGA GATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATG ACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAG TCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTA CTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGG CCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCG CCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGAC GAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGC CGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGAC GGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCT TGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAA AGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCC CAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGT CATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGG GCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGA GGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAA ACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGC TGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGA GAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCA AACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAG ACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

FTREKTKAPRGEVKACPSLHRGTAQPAVNSVLW TFFFF*DRVLFCRPGWSAEVRSRLTAASASRVQV IILLQPPQ*LGLQVPATTPS*VFVFLVETGFHR VSQDGLDLLTS*STRLGLPKCWDYRHEPLHLAA LWTSFFFFFFLRRSLTLSPRLECSGAISAHCKLR LPGSRHSPASASRVAGTTGARHHTQLSFCIFSR DGVSLC*PGWSRSPDLMIRPPRSPKVLGLQA*A TAPGQPCGLLSQRVPLLLP*TTLSLLSTPAGILE MAASIWSLS*LPTPMSPLTGMTHIAKSYPENTM TTSACRLLAKKP*PLPAQLSPGALFWALWAARA VLRSWSTWNLDSEPWAFPL*GCCSLRSSPASLAY FLRWMCGCRWHVHVSPLTGAQPSPSRC*HPMRR PWL*GTFPGSSPTRWTSTLAAPWGPGR*TTART AVPTPRAGPRGGRCRRGPRVPLRPWLARTAAAGT RRWRRWLLDALPGLRVLPSGGAASRLVVFRAGG RRFLRIGRARRLQGPGGITGDGGTGTEQAGAF* RPSWFQPRGCASSPCVCPGPGRRSPLPLATVYPL GVWFLSPGHWVQGIHPAKALVIAFAPCFWGH*Q SHPLSGECATGQPISRSHHPHPLWWPLHSSSCT PASGLPQVLGPVCRVKDQEMSLLLHPAPLGAASV IHALPGPSTHCCECASPEPVKRQLSILETQIIR ALPCTEEKGSTRP*WTLTCENANMNMVGEPWIR RVTWGRQRLAPW*LPHNKVVIWILSIFFLVHTEK HFIMEVGFLAIYIVFWAAPSREVSPVVSPRTGC CMGAHTHVIGGSFKSQTEGKEA*VAQSGRNIHL RSFGKTSPGKAA*TTVI*SSSIQITESCVLVFSQ TLDVLPASP*VL*PR*TMSLKGGSMPDLRWRPK TVQNKQLLPKLTP

Frame 1

SHVRKPRLQEVK*RLVPVYTEAQHSQL*TQSSG LFFFFETESCSVAQAGVQRCDLGSLQPLPPGFK *LSCFSLPSSWDYRCPPPRPAKFLYF**RQGFTV LARMVLIS*PRDPPASASQSVGITGMSHCTWQP CGLLFFFFSF*DGVSLCHPGWSAVARSQLTASY ASRVHAILLPQPPE*LGLQAPVTTPS*VFVFLVE TGFHCVSQDGLDLLTS*SARLGLPKCWDYRREP PRPASPVDF*ASECLFCCPKPHLASFPPLQVSW RWPLPSGVCHDCQPQCPRLPV*PI*QSPIQRTL* PPAHAGCSPRSHSHCPLS*VLGPYSGHFGPPGQ S*DPGAPGISTPSLGPFLCEAAAL*DLPQQA*P TS*GGCVGAGGMSTSLH*LGHSLPQAAADTL*GG RGSEGHFLAAALPDGLLRWQLLGALDGEPRPGP PSPRPGPARAGEGAGGVRASPFGRGLRGLQLQG REGGAAGSLTRSRASGSCPPEEQPRGWWFSEQEA DVFSALEEPAVCRGLEESLGMVAQALNRLGPFD GLLGFSQGAALAALVCALGQAGDPRFPLPRFIL LVSGFCPRGIGFKESILQRPLSLPSLHVFGDTDK VIPSQESVQLASQFPGAITLTHSGGHFIPAAAP QRQAYLKFLDQFAE*KIKKCLCSYIQLL*GQPP SSMPSQDPPLTAVSAPHQNQLRDNYQFLRPKL*G PCPVLKKRGAQGLNGH*LVKTQT*IWLESPGLG G*HGEGRGWHHGDCHIIKW*FGF*ASFSWYTQK NIL*WKSVSWPST*FSGQRQAGRCLQL*VLGQAA AWVHILTLLVEVLSPKLKGRRPRWHNQGGIYI* EVLGRHHLARLPEPQ*FSLPLSRSLRVVCWSSL KPWMCYLLLPKCSNLAEL*A*RVGRCQTLGGGQR QYRINSFFLN*P

Frame 2

HT*ENQGSKR*SEGLSQFTQRHSTASCELSPLD FFFFLRQSLVLSPRLECRGAISAHCSLCLPGSS DYPASASPVAGTTGARHHAQLSFCIFSRDRVSP C*PGWS*SPDLVIHPPRPPKVLGLQA*ATAPGSP VDFFFFFFLFETESHSVTQAGVQWRDLSSLQAT PPGFTPFSCLSLPSSWDYRRPSPHPAKFLYF** RRGFTVLARMVSIS*PHDPPASVSQSAGITGVSH RARPALWTSKPASASSAALNHT*PPFHPCRYLG DGRFHLESVMIANPNVPAYRYDPYSKVLSREHY DHQRMQAARQEAIATARSAKSWGLILGTLGRQGS PKILEHLESRLRALGLSFVRLLLSEIFPSKLSL LPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEA AVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDR RPHAPGRPARGKVQEGSARPPSAVACEDCSCRD EKVAPLAP*RAPGPQGPALRRSSLEAGGFQSRR PTFSPHWKSPPSAGAWRNHWGWWHRH*TGWGLLT AFLVSAKGLR*QPLCVPWARQAIPASPCHGLSS WCLVSVPGALGSRNPSCKGPCHCLRSMFLGTLT KSSPLRRVCNWPANFPEPSPSPTLVATSFQQLHP SVRPTSSSWTSLQSERSRNVSAPTSSSSRGSLR HPCPPRTLHSLL*VRLTRTS*ETTINS*DPNYK GPALY*RKGEHKALMDIDL*KRKHEYGWRALD*E GDMGKAEAGTMVTAT**SGDLDFEHLFPGTHRK TFYNGSRFLGHLHSFLGSAKQGGVSSCESSDRL LHGCTYSRYWWKF*VPN*REGGLGGTIREEYTSE KFWEDITWQGCLNHSNLVFLYPDH*ELCAGLLS NPGCVTCFSLSALTSLNYEPEGWVDARP*VEAK DSTE*TASS*IDP

Sequence DPH1.kAug05

TCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGCAGATGTTATTGTCCCTCCCT CCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGA ACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTAGGCGGGGGCTTCCAGGAGGGAGGAGAGACCGCGCCTGGGCACTG GCCGCCGCCCTGCGACCGCGACGGGAAACGCACAGGAGCGGAGCGGAGGGAAACGCAGGGTCGCAGGAGCGAGACCCTAACCAAAGTCTGCGACCTCAGG TGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGG GCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCA GGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGC CCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAG GAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCC AATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGC AGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTG AGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCC TTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGA CTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACA TAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTT TAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGA ACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCT AACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCC A

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

SVAGDSVPFTGRRED*IQ*SRGRKTLADVIVPP SPPLGGRGSEGHFLAAALPDGLLRWQLLGALDGE PRPGPPSPRPGPARAGEGRRGLPGGRRDRAWAL AAALRPRRETHRSGAEGNAGSQERDPNQSLRPQ VQEGSARPPSAVACEDCSCRDEKVAPLAP*RAPG PQGPALRRSSLEAGGFQSRRPTFSPHWKSPPSA GAWRNHWGWWHRH*TGWGLLTAFLVSAKGLR*Q PLCVPWARQAIPASPCHGLSSWCLVSVPGALGSR NPSCKGPCHCLRSMFLGTLTKSSPLRRVCNWPA NFPEPSPSPTLVATSFQQLHPSVRPTSSSWTSL QSERSRNVSAPTSSSSRGSLRHPCPPRTLHSLL* VRLTRTS*ETTINS*DPNYKGPALY*RKGEHKA LMDIDL*KRKHEYGWRALD*EGDMGKAEAGTMV TAT**SGDLDFEHLFPGTHRKTFYNGSRFLGHLH SFLGSAKQGGVSSCESSDRLLHGCTYSRYWWKF *VPN*REGGLGGTIREEYTSEKFWEDITWQGCL NHSNLVFLYPDH*ELCAGLLSNPGCVTCFSLSAL TSLNYEPEGWVDARP*VEAKDSTE*TASS*IDP

Frame 1

LWQGTVCPSQAVVRTEFSNPGAEKHWQMLLSLP PLP*AAVALRDISWQQPYPMDFYAGSSLGPWTV NHGQDRRPHAPGRPARGKVGGGFQEGGETAPGHW PPPCDRDGKRTGAERRETQGRRSETLTKVCDLR CRRGPRVPLRPWLARTAAAGTRRWRRWLLDALP GLRVLPSGGAASRLVVFRAGGRRFLRIGRARRLQ GPGGITGDGGTGTEQAGAF*RPSWFQPRGCASS PCVCPGPGRRSPLPLATVYPLGVWFLSPGHWVQ GIHPAKALVIAFAPCFWGH*QSHPLSGECATGQP ISRSHHPHPLWWPLHSSSCTPASGLPQVLGPVC RVKDQEMSLLLHPAPLGAASVIHALPGPSTHCC ECASPEPVKRQLSILETQIIRALPCTEEKGSTRP *WTLTCENANMNMVGEPWIRRVTWGRQRLAPW* LPHNKVVIWILSIFFLVHTEKHFIMEVGFLAIY IVFWAAPSREVSPVVSPRTGCCMGAHTHVIGGSF KSQTEGKEA*VAQSGRNIHLRSFGKTSPGKAA* TTVI*SSSIQITESCVLVFSQTLDVLPASP*VL *PR*TMSLKGGSMPDLRWRPKTVQNKQLLPKLTP

Frame 2

CGRGQCALHRPS*GLNSVIQGQKNTGRCYCPSL PSPRRPWL*GTFPGSSPTRWTSTLAAPWGPGR* TTARTAVPTPRAGPRGGR*AGASRREERPRLGT GRRPATATGNAQERSGGKRRVAGARP*PKSATSG AGGVRASPFGRGLRGLQLQGREGGAAGSLTRSR ASGSCPPEEQPRGWWFSEQEADVFSALEEPAVC RGLEESLGMVAQALNRLGPFDGLLGFSQGAALAA LVCALGQAGDPRFPLPRFILLVSGFCPRGIGFK ESILQRPLSLPSLHVFGDTDKVIPSQESVQLAS QFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQFA E*KIKKCLCSYIQLL*GQPPSSMPSQDPPLTAV SAPHQNQLRDNYQFLRPKL*GPCPVLKKRGAQG LNGH*LVKTQT*IWLESPGLGG*HGEGRGWHHGD CHIIKW*FGF*ASFSWYTQKNIL*WKSVSWPST *FSGQRQAGRCLQL*VLGQAAAWVHILTLLVEV LSPKLKGRRPRWHNQGGIYI*EVLGRHHLARLPE PQ*FSLPLSRSLRVVCWSSLKPWMCYLLLPKCS NLAEL*A*RVGRCQTLGGGQRQYRINSFFLN*P

Sequence DPH1.lAug05

TTCACACGTGAGAAAACCAAGGCTCCAAGAGGTGAAGTGAAGGCTTGTCCCAGTTTACACAGAGGCACAGCACAGCCAGCTGTGAACTCAGTCCTCTGGA CTTTTTTTTTTTTTTGAGACAGAGTCTTGTTCTGTCGCCCAGGCTGGAGTGCAGAGGTGCGATCTCGGCTCACTGCAGCCTCTGCCTCCCGGGTTCAAGT GATTATCCTGCTTCAGCCTCCCCAGTAGCTGGGACTACAGGTGCCCGCCACCACGCCCAGCTAAGTTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGT GTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACTGCACCTGGCAGCCC TGTGGACTTCTTTTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAAGCTACG CCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGTCACCACACCCAGCTAAGTTTTTGTATTTTTAGTAGA GACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCCGCCCGCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA CCGCGCCCGGCCAGCCCTGTGGACTTCTAAGCCAGCGAGTGCCTCTTCTGCTGCCCTAAACCACACTTAGCCTCCTTTCCACCCCTGCAGGTATCTTGGA GATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATG ACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGCCCGCTCACACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCT GCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCT CCTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCC TTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGG AGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGAT GGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCA GGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGT CATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCAC CCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTC TGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAA GAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAAC GCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTT GGATTTTGAGCATCTTTTTCCTGGTACACACAGAAAAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAG GGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCTGCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAG GCCTAGGTGGCACAATCAGGGAGGAATATACATCTGAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTA TCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCTCAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAA GGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAAAGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

FTREKTKAPRGEVKACPSLHRGTAQPAVNSVLW TFFFF*DRVLFCRPGWSAEVRSRLTAASASRVQV IILLQPPQ*LGLQVPATTPS*VFVFLVETGFHR VSQDGLDLLTS*STRLGLPKCWDYRHEPLHLAA LWTSFFFFFFLRRSLTLSPRLECSGAISAHCKLR LPGSRHSPASASRVAGTTGARHHTQLSFCIFSR DGVSLC*PGWSRSPDLMIRPPRSPKVLGLQA*A TAPGQPCGLLSQRVPLLLP*TTLSLLSTPAGILE MAASIWSLS*LPTPMSPLTGMTHIAKSYPENTM TTSACRLLAKKP*PLPAHTWNLDSEPWAFPL*G CCSLRSSPASLAYFLRWMCGCRWHVHVSPLTGAQ PSPSRC*HPMRRPWL*GTFPGSSPTRWTSTLAA PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVP LRPWLARTAAAGTRRWRRWLLDALPGLRVLPSGG AASRLVVFRAGGRRFLRIGRARRLQGPGGITGD GGTGTEQAGAF*RPSWFQPRGCASSPCVCPGPG RRSPLPLATVYPLGVWFLSPGHWVQGIHPAKALV IAFAPCFWGH*QSHPLSGECATGQPISRSHHPH PLWWPLHSSSCTPASGLPQVLGPVCRVKDQEMS LLLHPAPLGAASVIHALPGPSTHCCECASPEPVK RQLSILETQIIRALPCTEEKGSTRP*WTLTCEN ANMNMVGEPWIRRVTWGRQRLAPW*LPHNKVVI WILSIFFLVHTEKHFIMEVGFLAIYIVFWAAPSR EVSPVVSPRTGCCMGAHTHVIGGSFKSQTEGKE A*VAQSGRNIHLRSFGKTSPGKAA*TTVI*SSS IQITESCVLVFSQTLDVLPASP*VL*PR*TMSLK GGSMPDLRWRPKTVQNKQLLPKLTP

Frame 1

SHVRKPRLQEVK*RLVPVYTEAQHSQL*TQSSG LFFFFETESCSVAQAGVQRCDLGSLQPLPPGFK *LSCFSLPSSWDYRCPPPRPAKFLYF**RQGFTV LARMVLIS*PRDPPASASQSVGITGMSHCTWQP CGLLFFFFSF*DGVSLCHPGWSAVARSQLTASY ASRVHAILLPQPPE*LGLQAPVTTPS*VFVFLVE TGFHCVSQDGLDLLTS*SARLGLPKCWDYRREP PRPASPVDF*ASECLFCCPKPHLASFPPLQVSW RWPLPSGVCHDCQPQCPRLPV*PI*QSPIQRTL* PPAHAGCSPRSHSHCPLTPGISTPSLGPFLCEA AAL*DLPQQA*PTS*GGCVGAGGMSTSLH*LGH SLPQAAADTL*GGRGSEGHFLAAALPDGLLRWQL LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP FGRGLRGLQLQGREGGAAGSLTRSRASGSCPPE EQPRGWWFSEQEADVFSALEEPAVCRGLEESLGM VAQALNRLGPFDGLLGFSQGAALAALVCALGQA GDPRFPLPRFILLVSGFCPRGIGFKESILQRPL SLPSLHVFGDTDKVIPSQESVQLASQFPGAITLT HSGGHFIPAAAPQRQAYLKFLDQFAE*KIKKCL CSYIQLL*GQPPSSMPSQDPPLTAVSAPHQNQL RDNYQFLRPKL*GPCPVLKKRGAQGLNGH*LVKT QT*IWLESPGLGG*HGEGRGWHHGDCHIIKW*F GF*ASFSWYTQKNIL*WKSVSWPST*FSGQRQA GRCLQL*VLGQAAAWVHILTLLVEVLSPKLKGRR PRWHNQGGIYI*EVLGRHHLARLPEPQ*FSLPL SRSLRVVCWSSLKPWMCYLLLPKCSNLAEL*A* RVGRCQTLGGGQRQYRINSFFLN*P

Frame 2

HT*ENQGSKR*SEGLSQFTQRHSTASCELSPLD FFFFLRQSLVLSPRLECRGAISAHCSLCLPGSS DYPASASPVAGTTGARHHAQLSFCIFSRDRVSP C*PGWS*SPDLVIHPPRPPKVLGLQA*ATAPGSP VDFFFFFFLFETESHSVTQAGVQWRDLSSLQAT PPGFTPFSCLSLPSSWDYRRPSPHPAKFLYF** RRGFTVLARMVSIS*PHDPPASVSQSAGITGVSH RARPALWTSKPASASSAALNHT*PPFHPCRYLG DGRFHLESVMIANPNVPAYRYDPYSKVLSREHY DHQRMQAARQEAIATARSHLESRLRALGLSFVRL LLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGT AFPKPLLTPYEAAVALRDISWQQPYPMDFYAGS SLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP SAVACEDCSCRDEKVAPLAP*RAPGPQGPALRR SSLEAGGFQSRRPTFSPHWKSPPSAGAWRNHWG WWHRH*TGWGLLTAFLVSAKGLR*QPLCVPWARQ AIPASPCHGLSSWCLVSVPGALGSRNPSCKGPC HCLRSMFLGTLTKSSPLRRVCNWPANFPEPSPS PTLVATSFQQLHPSVRPTSSSWTSLQSERSRNVS APTSSSSRGSLRHPCPPRTLHSLL*VRLTRTS* ETTINS*DPNYKGPALY*RKGEHKALMDIDL*K RKHEYGWRALD*EGDMGKAEAGTMVTAT**SGDL DFEHLFPGTHRKTFYNGSRFLGHLHSFLGSAKQ GGVSSCESSDRLLHGCTYSRYWWKF*VPN*REG GLGGTIREEYTSEKFWEDITWQGCLNHSNLVFLY PDH*ELCAGLLSNPGCVTCFSLSALTSLNYEPE GWVDARP*VEAKDSTE*TASS*IDP

Sequence DPH1.mAug05

TCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCA TGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCA AGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTC AGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAGGTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCT GCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCC TACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGC GGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAACCACGGCCAGGAC CGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGG ACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAG GCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTG ACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCT CTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGAC AAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCAC CCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCC GTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAA GGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAG GAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAAAGTGGTGATTTGGATTTTGAGCATCTTTTTCCTGGTACACACAGAA AAACATTTTATAATGGAAGTCGGTTTCTTGGCCATCTACATAGTTTTCTGGGCAGCGCCAAGCAGGGAGGTGTCTCCAGTTGTGAGTCCTCGGACAGGCT GCTGCATGGGTGCACATACTCACGTTATTGGTGGAAGTTTTAAGTCCCAAACTGAAGGGAAGGAGGCCTAGGTGGCACAATCAGGGAGGAATATACATCT GAGAAGTTTTGGGAAGACATCACCTGGCAAGGCTGCCTGAACCACAGTAATTTAGTCTTCCTCTATCCAGATCACTGAGAGTTGTGTGCTGGTCTTCTCT CAAACCCTGGATGTGTTACCTGCTTCTCCCTAAGTGCTCTAACCTCGCTGAACTATGAGCCTGAAGGGTGGGTCGATGCCAGACCTTAGGTGGAGGCCAA AGACAGTACAGAATAAACAGCTTCTTCCTAAATTGACCCCA

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

SPGEILGCTSPRLSKEVEAVVYLGDGRFHLESV MIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQ EAIATARSAKSWGLILGTLGRQGSPKILEVSGL RTASGGGK*LGTQLGKPVGHRFSFGCLTR*PPP CLPLPTAPGISTPSLGPFLCEAAAL*DLPQQA*P TS*GGCVGAGGMSTSLH*LGHSLPQAAADTL*G GRGSEGHFLAAALPDGLLRWQLLGALDGEPRPG PPSPRPGPARAGEGAGGVRASPFGRGLRGLQLQG REGGAAGSLTRSRASGSCPPEEQPRGWWFSEQE ADVFSALEEPAVCRGLEESLGMVAQALNRLGPF DGLLGFSQGAALAALVCALGQAGDPRFPLPRFIL LVSGFCPRGIGFKESILQRPLSLPSLHVFGDTD KVIPSQESVQLASQFPGAITLTHSGGHFIPAAA PQRQAYLKFLDQFAE*KIKKCLCSYIQLL*GQPP SSMPSQDPPLTAVSAPHQNQLRDNYQFLRPKL* GPCPVLKKRGAQGLNGH*LVKTQT*IWLESPGL GG*HGEGRGWHHGDCHIIKW*FGF*ASFSWYTQK NIL*WKSVSWPST*FSGQRQAGRCLQL*VLGQA AAWVHILTLLVEVLSPKLKGRRPRWHNQGGIYI *EVLGRHHLARLPEPQ*FSLPLSRSLRVVCWSSL KPWMCYLLLPKCSNLAEL*A*RVGRCQTLGGGQ RQYRINSFFLN*P

Frame 1

PLERSWAAHPPDCPKRWRPLCILEMAASIWSLS *LPTPMSPLTGMTHIAKSYPENTMTTSACRLLA KKP*PLPAQLSPGALFWALWAARAVLRSWRSVGS GQPLEEGSDWEHSWENQ*ATGSALAA*PGDPHP ASLSQQHLESRLRALGLSFVRLLLSEIFPSKLS LLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEA AVALRDISWQQPYPMDFYAGSSLGPWTVNHGQD RRPHAPGRPARGKVQEGSARPPSAVACEDCSCR DEKVAPLAP*RAPGPQGPALRRSSLEAGGFQSRR PTFSPHWKSPPSAGAWRNHWGWWHRH*TGWGLL TAFLVSAKGLR*QPLCVPWARQAIPASPCHGLS SWCLVSVPGALGSRNPSCKGPCHCLRSMFLGTLT KSSPLRRVCNWPANFPEPSPSPTLVATSFQQLH PSVRPTSSSWTSLQSERSRNVSAPTSSSSRGSL RHPCPPRTLHSLL*VRLTRTS*ETTINS*DPNYK GPALY*RKGEHKALMDIDL*KRKHEYGWRALD* EGDMGKAEAGTMVTAT**SGDLDFEHLFPGTHR KTFYNGSRFLGHLHSFLGSAKQGGVSSCESSDRL LHGCTYSRYWWKF*VPN*REGGLGGTIREEYTS EKFWEDITWQGCLNHSNLVFLYPDH*ELCAGLL SNPGCVTCFSLSALTSLNYEPEGWVDARP*VEAK DSTE*TASS*IDP

Frame 2

PWRDPGLHIPPTVQRGGGRCVSWRWPLPSGVCH DCQPQCPRLPV*PI*QSPIQRTL*PPAHAGCSP RSHSHCPLS*VLGPYSGHFGPPGQS*DPGGQWA QDSLWRREVTGNTAGKTSRPQVQLWLLDQVTPTL PPSPNSTWNLDSEPWAFPL*GCCSLRSSPASLA YFLRWMCGCRWHVHVSPLTGAQPSPSRC*HPMR RPWL*GTFPGSSPTRWTSTLAAPWGPGR*TTART AVPTPRAGPRGGRCRRGPRVPLRPWLARTAAAG TRRWRRWLLDALPGLRVLPSGGAASRLVVFRAG GRRFLRIGRARRLQGPGGITGDGGTGTEQAGAF* RPSWFQPRGCASSPCVCPGPGRRSPLPLATVYP LGVWFLSPGHWVQGIHPAKALVIAFAPCFWGH* QSHPLSGECATGQPISRSHHPHPLWWPLHSSSCT PASGLPQVLGPVCRVKDQEMSLLLHPAPLGAAS VIHALPGPSTHCCECASPEPVKRQLSILETQII RALPCTEEKGSTRP*WTLTCENANMNMVGEPWIR RVTWGRQRLAPW*LPHNKVVIWILSIFFLVHTE KHFIMEVGFLAIYIVFWAAPSREVSPVVSPRTG CCMGAHTHVIGGSFKSQTEGKEA*VAQSGRNIHL RSFGKTSPGKAA*TTVI*SSSIQITESCVLVFS QTLDVLPASP*VL*PR*TMSLKGGSMPDLRWRP KTVQNKQLLPKLTP

Sequence DPH1.eAug05

CTTTGGGCCGCCAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGAGATCTT CCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTG CTGACACCCTATGAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGG TGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGA GGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGGGTGCGCCCCGCCTTTTG CCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCTTCCGCTCGGGGAAAGAC CGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCGGCAGAGCGAGCGGGGCT TCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCCGACCCCCCGGGCCCCGA GGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGACACCCTTCAGCATCCCCC ACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGCCTGTCCTCCCAGGCTTC CGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCTCGATTCCCTTTTTCTCT CTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCATATCGCTGTAGGGTCCTG CCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCTGCAGGGGCCTGGAGGAA TCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGCAGCCCTTGTGTGTGCCC TGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTCAAGGAATCCATCCTGCA AAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCC ATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTTTGCAGAGTGAAAGATCA AGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCTGTGAGTGCGCCTCACCA GAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGA CTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGGTGACTGCCACATAATAA AGTGGTGATTTGG

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

LWAARAVLRSWSTWNLDSEPWAFPL*GCCSLRS SPASLAYFLRWMCGCRWHVHVSPLTGAQPSPSRC *HPMRRPWL*GTFPGSSPTRWTSTLAAPWGPGR *TTARTAVPTPRAGPRGGRCRRGPRVPLRPWLA RTAAAGTRRWRRWLLDALPGLRYQPPLWVRPAFC RCHGNALALRGPPSGRC*RSVPPRALCFRSGKD RFRCFRRSLPGIMAAQRPLRVLCLAGFRQSERG FREKTGALRKALRGRAELVCLSGPHPVPDPPGPE GARSDFGETSPAPETHSVSSILFRPPRHPSASP TLRPPAFLPPSPHPSFLATHTLFAQTAACPPRL PPSPSSSAVSCSGQSTHFPPLISPRLLPRFPFSL CHLEPSCPFSFLSFLEPPMP*SHPFSSYRCRVL PSGGAASRLVVFRAGGRRFLRIGRARRLQGPGG ITGDGGTGTEQAGAF*RPSWFQPRGCASSPCVCP GPGRRSPLPLATVYPLGVWFLSPGHWVQGIHPA KALVIAFAPCFWGH*QSHPLSGECATGQPISRS HHPHPLWWPLHSSSCTPASGLPQVLGPVCRVKDQ EMSLLLHPAPLGAASVIHALPGPSTHCCECASP EPVKRQLSILETQIIRALPCTEEKGSTRP*WTL TCENANMNMVGEPWIRRVTWGRQRLAPW*LPHNK VVIW

Frame 1

FGPPGQS*DPGAPGISTPSLGPFLCEAAAL*DL PQQA*PTS*GGCVGAGGMSTSLH*LGHSLPQAA ADTL*GGRGSEGHFLAAALPDGLLRWQLLGALDG EPRPGPPSPRPGPARAGEGAGGVRASPFGRGLR GLQLQGREGGAAGSLTRSRASGISPRSGCAPPF AVVMGTPWRSEARPRGGADVRFRPVHCASARGKT ASGASVAPCRA*WPRSDPCGSCAWRASGRASGA SVRRPGR*GRRCGVAPSSCASAARTRSPTPRAP RAPDQTSVRQAPLRKRTQFPPSCFVPLDTLQHPP PCVHPHSSHPVHTLHFSRPIPSLLKLQPVLPGF RLHRLPPLSPVQANPLISPLSFPPDFCLDSLFL SVISNPPALSHFFPSWSLPCPNPILFPHIAVGSC PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEE SLGMVAQALNRLGPFDGLLGFSQGAALAALVCA LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ RPLSLPSLHVFGDTDKVIPSQESVQLASQFPGA ITLTHSGGHFIPAAAPQRQAYLKFLDQFAE*KI KKCLCSYIQLL*GQPPSSMPSQDPPLTAVSAPHQ NQLRDNYQFLRPKL*GPCPVLKKRGAQGLNGH* LVKTQT*IWLESPGLGG*HGEGRGWHHGDCHII KW*F

Frame 2

LGRQGSPKILEHLESRLRALGLSFVRLLLSEIF PSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPL LTPYEAAVALRDISWQQPYPMDFYAGSSLGPWT VNHGQDRRPHAPGRPARGKVQEGSARPPSAVACE DCSCRDEKVAPLAP*RAPGPQVSAPALGAPRLL PLSWERLGAPRPALGAVLTFGSAPCIVLPLGER PLPVLPSLLAGHNGRAATPAGPVPGGLPAERAGL P*EDRGAEEGAAGSRRARVPQRPAPGPRPPGPR GRQIRLR*DKPRSGNALSFLHLVSSPSTPFSIP HPASTRIPPTQSTPFISRDPYPLCSNCSLSSQAS AFTVFLRCLLFRPIHSFPPSHFPQTFASIPFFS LSSRTLLPFLISFLPGASHALIPSFFLISL*GP ALRRSSLEAGGFQSRRPTFSPHWKSPPSAGAWRN HWGWWHRH*TGWGLLTAFLVSAKGLR*QPLCVP WARQAIPASPCHGLSSWCLVSVPGALGSRNPSC KGPCHCLRSMFLGTLTKSSPLRRVCNWPANFPEP SPSPTLVATSFQQLHPSVRPTSSSWTSLQSERS RNVSAPTSSSSRGSLRHPCPPRTLHSLL*VRLT RTS*ETTINS*DPNYKGPALY*RKGEHKALMDID L*KRKHEYGWRALD*EGDMGKAEAGTMVTAT** SGDL

Sequence DPH1.fAug05

GCCAGGGCAGTCCTAAGATCCTGGAGGTCAGTGGGCTCAGGACAGCCTCTGGAGGAGGGAAGTGACTGGGAACACAGCTGGGAAAACCAGTAGGCCACAG GTTCAGCTTTGGCTGCTTGACCAGGTGACCCCCACCCTGCCTCCCTCTCCCAACAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAG GCTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGAGTATCTGCCTGGCTATGACTGGCTAAAGAAGCTTAGAAG CTGGGTCTTGCCCAGCTCGTCTTGAAGGCTGGAGCCAACAGGAATTGCTTCCATGGTCATGGAATTTTACTGTTTACAGAGACCTCCCTGGGAGGGCTAA CTGGGTCATACCTGCTTTGGGACTTTGGGGCTCAGAGGCAATGGCAACATCACATTTGTCCTTGTGAGATGAGTTTGAGCGGTGGCTGACACCTGCCCTG TACCAGGTACTAAATGTGGTTCTGGGGATACTATCACCAGTGAGCTGCCCTGGAAAGCTCATTGTCGGCAGAGGAAACAGGAGAAAACACGCAACAATAC AGTATTCCAAACAGCAGTGGGTCACTGTCTTTACTTTAGGATTCATAAAGCCTGTTAATGAATGGGTGTGCTGGGGGCACTGTCATGTTCTTCAGCATGC TGCCTGGGCGACGAGGAGGCCCTTCCTAGGGTCTGACCTGGCTTCCCTTCCCAGGTGGGTGCAGGTGGCATGTCCACGTCTCTCCATTGACTGGGGCACA GCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGTAACACCAAGCTCTGGGAGAGAGTGGGCTTTGGACGTGGTTCTCAAAGGTGAGGTCTGGAGTGGAG TGGGGTGGGTGGGCAGCACTTCGTTATGTTCGTAGATTCCGGAGTCTGTCTCGATTTCTCCAGCGGAGTCACTTCCTAGCTGTGTAGCCTTAGGCAAGGC CCTGAACCACACAGTTCCCTTCTGTGGCAGGGGACAGTGTGCCCTTCACAGGCCGTCGTGAGGACTGAATTCAGTAATCCAGGGGCAGAAAAACACTGGC AGATGTTATTGTCCCTCCCTCCCCTCCCCTAGGCGGCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTC CTTGGGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCGCGTCCCCCT TCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTTGACGCGCTCCCGGGCCTCAGGTATCAGCCCCCGCTCTGG GTGCGCCCCGCCTTTTGCCGTTGTCATGGGAACGCCTTGGCGCTCCGAGGCCCGCCCTCGGGGCGGTGCTGACGTTCGGTTCCGCCCCGTGCATTGTGCT TCCGCTCGGGGAAAGACCGCTTCCGGTGCTTCCGTCGCTCCTTGCCGGGCATAATGGCCGCGCAGCGACCCCTGCGGGTCCTGTGCCTGGCGGGCTTCCG GCAGAGCGAGCGGGGCTTCCGTGAGAAGACCGGGGCGCTGAGGAAGGCGCTGCGGGGTCGCGCCGAGCTCGTGTGCCTCAGCGGCCCGCACCCGGTCCCC GACCCCCCGGGCCCCGAGGGCGCCAGATCAGACTTCGGTGAGACAAGCCCCGCTCCGGAAACGCACTCAGTTTCCTCCATCTTGTTTCGTCCCCCTCGAC ACCCTTCAGCATCCCCCACCCTGCGTCCACCCGCATTCCTCCCACCCAGTCCACACCCTTCATTTCTCGCGACCCATACCCTCTTTGCTCAAACTGCAGC CTGTCCTCCCAGGCTTCCGCCTTCACCGTCTTCCTCCGCTGTCTCCTGTTCAGGCCAATCCACTCATTTCCCCCCTCTCATTTCCCCCAGACTTTTGCCT CGATTCCCTTTTTCTCTCTGTCATCTCGAACCCTCCTGCCCTTTCTCATTTCTTTCCTTCCTGGAGCCTCCCATGCCCTAATCCCATCCTTTTTCCTCAT ATCGCTGTAGGGTCCTGCCCTCCGGAGGAGCAGCCTCGAGGCTGGTGGTTTTCAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCCGTCT GCAGGGGCCTGGAGGAATCACTGGGGATGGTGGCACAGGCACTGAACAGGCTGGGGCCTTTTGACGGCCTTCTTGGTTTCAGCCAAGGGGCTGCGCTAGC AGCCCTTGTGTGTGCCCTGGGCCAGGCAGGCGATCCCCGCTTCCCCTTGCCACGGTTTATCCTCTTGGTGTCTGGTTTCTGTCCCCGGGGCATTGGGTTC AAGGAATCCATCCTGCAAAGGCCCTTGTCATTGCCTTCGCTCCATGTTTTTGGGGACACTGACAAAGTCATCCCCTCTCAGGAGAGTGTGCAACTGGCCA GCCAATTTCCCGGAGCCATCACCCTCACCCACTCTGGTGGCCACTTCATTCCAGCAGCTGCACCCCAGCGTCAGGCCTACCTCAAGTTCTTGGACCAGTT TGCAGAGTGAAAGATCAAGAAATGTCTCTGCTCCTACATCCAGCTCCTCTAGGGGCAGCCTCCGTCATCCATGCCCTCCCAGGACCCTCCACTCACTGCT GTGAGTGCGCCTCACCAGAACCAGTTAAGAGACAACTATCAATTCTTGAGACCCAAATTATAAGGGCCCTGCCCTGTACTGAAGAAAAGGGGAGCACAAG GCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAGCCCTGGATTAGGAGGGTGACATGGGGAAGGCAGAGGCTGGCACCATGG TGACTGCCACATAATAAAGTGGTGATTTGG

DPH1.F2 (forward primer)
DPH1.R2 (reverse primer)
Intron retention

Frame 0

ARAVLRSWRSVGSGQPLEEGSDWEHSWENQ*AT GSALAA*PGDPHPASLSQQHLESRLRALGLSFVR LLLSEIFPSKLSLLPEVDV*VSAWL*LAKEA*K LGLAQLVLKAGANRNCFHGHGILLFTETSLGGL TGSYLLWDFGAQRQWQHHICPCEMSLSGG*HLPC TRY*MWFWGYYHQ*AALESSLSAEETGENTQQY SIPNSSGSLSLL*DS*SLLMNGCAGGTVMFFSM LPGRRGGPS*GLTWLPFPGGCRWHVHVSPLTGAQ PSPSRC*HPMR*HQALGESGLWTWFSKVRSGVE WGGWAALRYVRRFRSLSRFLQRSHFLAV*P*AR P*TTQFPSVAGDSVPFTGRRED*IQ*SRGRKTLA DVIVPPSPPLGGRGSEGHFLAAALPDGLLRWQL LGALDGEPRPGPPSPRPGPARAGEGAGGVRASP FGRGLRGLQLQGREGGAAGSLTRSRASGISPRSG CAPPFAVVMGTPWRSEARPRGGADVRFRPVHCA SARGKTASGASVAPCRA*WPRSDPCGSCAWRAS GRASGASVRRPGR*GRRCGVAPSSCASAARTRSP TPRAPRAPDQTSVRQAPLRKRTQFPPSCFVPLD TLQHPPPCVHPHSSHPVHTLHFSRPIPSLLKLQ PVLPGFRLHRLPPLSPVQANPLISPLSFPPDFCL DSLFLSVISNPPALSHFFPSWSLPCPNPILFPH IAVGSCPPEEQPRGWWFSEQEADVFSALEEPAV CRGLEESLGMVAQALNRLGPFDGLLGFSQGAALA ALVCALGQAGDPRFPLPRFILLVSGFCPRGIGF KESILQRPLSLPSLHVFGDTDKVIPSQESVQLA SQFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQF AE*KIKKCLCSYIQLL*GQPPSSMPSQDPPLTA VSAPHQNQLRDNYQFLRPKL*GPCPVLKKRGAQ GLNGH*LVKTQT*IWLESPGLGG*HGEGRGWHHG DCHIIKW*F

Frame 1

PGQS*DPGGQWAQDSLWRREVTGNTAGKTSRPQ VQLWLLDQVTPTLPPSPNSTWNLDSEPWAFPL* GCCSLRSSPASLAYFLRWMCEYLPGYDWLKKLRS WVLPSSS*RLEPTGIASMVMEFYCLQRPPWEG* LGHTCFGTLGLRGNGNITFVLVR*V*AVADTCP VPGTKCGSGDTITSELPWKAHCRQRKQEKTRNNT VFQTAVGHCLYFRIHKAC**MGVLGALSCSSAC CLGDEEALPRV*PGFPSQVGAGGMSTSLH*LGH SLPQAAADTL*GNTKLWERVGFGRGSQR*GLEWS GVGGQHFVMFVDSGVCLDFSSGVTS*LCSLRQG PEPHSSLLWQGTVCPSQAVVRTEFSNPGAEKHW QMLLSLPPLP*AAVALRDISWQQPYPMDFYAGSS LGPWTVNHGQDRRPHAPGRPARGKVQEGSARPP SAVACEDCSCRDEKVAPLAP*RAPGPQVSAPAL GAPRLLPLSWERLGAPRPALGAVLTFGSAPCIVL PLGERPLPVLPSLLAGHNGRAATPAGPVPGGLP AERAGLP*EDRGAEEGAAGSRRARVPQRPAPGP RPPGPRGRQIRLR*DKPRSGNALSFLHLVSSPST PFSIPHPASTRIPPTQSTPFISRDPYPLCSNCS LSSQASAFTVFLRCLLFRPIHSFPPSHFPQTFA SIPFFSLSSRTLLPFLISFLPGASHALIPSFFLI SL*GPALRRSSLEAGGFQSRRPTFSPHWKSPPS AGAWRNHWGWWHRH*TGWGLLTAFLVSAKGLR* QPLCVPWARQAIPASPCHGLSSWCLVSVPGALGS RNPSCKGPCHCLRSMFLGTLTKSSPLRRVCNWP ANFPEPSPSPTLVATSFQQLHPSVRPTSSSWTS LQSERSRNVSAPTSSSSRGSLRHPCPPRTLHSLL *VRLTRTS*ETTINS*DPNYKGPALY*RKGEHK ALMDIDL*KRKHEYGWRALD*EGDMGKAEAGTM VTAT**SGDL

Frame 2

QGSPKILEVSGLRTASGGGK*LGTQLGKPVGHR FSFGCLTR*PPPCLPLPTAPGISTPSLGPFLCE AAAL*DLPQQA*PTS*GGCVSICLAMTG*RSLE AGSCPARLEGWSQQELLPWSWNFTVYRDLPGRAN WVIPALGLWGSEAMATSHLSL*DEFERWLTPAL YQVLNVVLGILSPVSCPGKLIVGRGNRRKHATI QYSKQQWVTVFTLGFIKPVNEWVCWGHCHVLQHA AWATRRPFLGSDLASLPRWVQVACPRLSIDWGT AFPKPLLTPYEVTPSSGREWALDVVLKGEVWSG VGWVGSTSLCS*IPESVSISPAESLPSCVALGKA LNHTVPFCGRGQCALHRPS*GLNSVIQGQKNTG RCYCPSLPSPRRPWL*GTFPGSSPTRWTSTLAA PWGPGR*TTARTAVPTPRAGPRGGRCRRGPRVPL RPWLARTAAAGTRRWRRWLLDALPGLRYQPPLW VRPAFCRCHGNALALRGPPSGRC*RSVPPRALC FRSGKDRFRCFRRSLPGIMAAQRPLRVLCLAGFR QSERGFREKTGALRKALRGRAELVCLSGPHPVP DPPGPEGARSDFGETSPAPETHSVSSILFRPPR HPSASPTLRPPAFLPPSPHPSFLATHTLFAQTAA CPPRLPPSPSSSAVSCSGQSTHFPPLISPRLLP RFPFSLCHLEPSCPFSFLSFLEPPMP*SHPFSS YRCRVLPSGGAASRLVVFRAGGRRFLRIGRARRL QGPGGITGDGGTGTEQAGAF*RPSWFQPRGCAS SPCVCPGPGRRSPLPLATVYPLGVWFLSPGHWV QGIHPAKALVIAFAPCFWGH*QSHPLSGECATGQ PISRSHHPHPLWWPLHSSSCTPASGLPQVLGPV CRVKDQEMSLLLHPAPLGAASVIHALPGPSTHC CECASPEPVKRQLSILETQIIRALPCTEEKGSTR P*WTLTCENANMNMVGEPWIRRVTWGRQRLAPW *LPHNKVVIW