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Sequences

Sequence ECGF1.bAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGAGCGATGGCA GCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGC TCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGC ACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTG GAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCAT GTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAG CCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGA GATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGT TCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGC GCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTA AGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACG ACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCG GCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCAC GAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCC CCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGC CCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGC TGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCA TGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTT CCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGG GTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAG CAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGC TGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACC TCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAA GCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCG GGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCT CTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAA TGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGT GTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAG ATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATC CATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTG AGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGT TCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGG CCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGC CCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTG TGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCA GGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCT GACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTT GAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPQTPDVSGIPAGPERWQ P**PREPGPHPRLVTSPGKGARDFPTLRQSPSS SRS*SA*SETEAA*AKRTSGASWPLW*MGARRA HRSGPC*WPSDFGAWIWRRPRC*PRPWLSRDSSW SGQRPGASSLWTSIPQGVWVTRSAWSSHLPWRH VAARCQ*SADVVWGTQEAPWISWSLFLDSMSSR AQSRCKCCWTRRAAVSWVRVSSWFLRTESYMQPE M*QPPWTACHSSQPPFSVRNSWRGCPLWWWTLS SEGPPSSPTRSRPGSWQRRWLAWEPA*GFGSRQ R*PPWTSPWVAAWATPWRWRRRCSAWTAQARQT* GTWSPRSGAPCSGSADTRGLRPRALPGWPRRWT TARPLAASSGCWRRRAWIPVWPEPCAREVPQNA GSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWCCT SSGPGAAALGSRSAWGWAQSCWSTWVRGCAVGP PGSACTGTAPRSAARRAAPCRRRSYSPTARHSP PPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGSRA AEAGAVSMNT*PPAGSAFRARAESLSAAAPVCA CAAPGTPAPCL*ACGARFP*AGGAERRELEVGA SSRAWSTDARPRREVRTPGLGGEEVALRTRWTPG *FPSSGYCAGLAGHTPGRAGGRSEAGAPVGGSQ QAPVFSRPSTVITRSRGASSGPGTFLATRALAL LGRAQTQNRPGGGAVSPREGALSRRGQ*PGVLRP PPSKPSAGARGKGSKVTNAKAGRRL*ARRSCWE AWM*GGWGFVAGGSVVRLCQERLNTAGGQSLSS GGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNRLL SGIASQRVTHLLLRNGFIANR*RG*FRQGMHLE CFPSARGSPC*GAGAGQGAVTGW*SQVEDRSDR CGCPGVDVWWRWSSELR*AAANAC*A*TWAGSFR WVAGLKSQGQPRHCPGPSLLAPDACACFQEHQI HAAADSEPHSLAQALSAQASGPPWDPGRPGPAS EVLAFVKAGPCRDRWAGPAPGPWASNPAADHRPV RGWTRWGLAGPEG*EGEAAAAKANRSPAPGSCG PGRLPPAGSQRPGSLQG*LPGPVGADVLWLHSL P*HLPRRAGEAGAGGAAAGSRAWFASSAACLHHC GPRAGRR*SHGPLRPGLPPKTVGSDRLHQTGCP G*SQLPRVLQCRPQG*GPGLHRGPLHCHLPAQP *RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCPVL SHCSLGPIIKRAAF

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGRRPRTCQASPQARSDG SLDDPGNRGPTRAW*LLRGREPGTSRPFARAQAA PGADPHEARRRPPERSGHQGLRGRCGEWERAGR TDRGHADGHPTSGHGSGGDLGADPGPGSVGTAA GVARGLAPAACGQAFHRGCG*QGQPGPRTCPGGM WLQGANDQRTWSGAHRRHLG*AGVYSWIQCHPE PRADASAAGPGGLLYRGSE*AAGSCGRNPICSQ RCDSHRGQPATHHSLHSQ*ETRGGAVRSGGGR*V RRGRRLPQPGAGPGAGKDAGWRGSQPRASGRGS ADRHGQAPGSLRGPRPGGGGGAALHGRRRPARL KGPGHHARGRPALAQRTRGDSGPGRCPGGRGAGR RLGPWPLRADAGGAGRGSRSGPSPVLGKSRRTP AAAASRPGAGGAAGARRWHRGAGPGAAAGAGAA RARGRAQPRWGAAPPGGGRRAAGRRGSEAAPWDP LAPRAPGRPRAQRPAEPRPAGGARTLRPRAIRR PLALRRARSAAAAIKLLCRETLSVLGRERKDPG LRRRGPSR*TRDPRRAPPSAHALRACQRLRPCAH AQLRGRLRPACERVAPAFPEPAGQSAGSWRSAL PLVLGPLTRGPAARCGRRGWEGRR*P*GLAGLR GSFPAPATARGWRGTPQGALEAGARLGRPWEAPS RHRCSRGQAQL*RARAALRVVLEPFWPRGRWPC WGGHKPRTARAGVQ*VLGRVPSAGGGSDRES*D LHLANLLRGPVGKAQRSPTQRQGVGCEPGGAAGK PGCEEGGVLWRVEVSCVSARRG*TQPAGRV*AP GVGRAGFLLGVWRKAAVG*SGWR*QESVGGIGC SRGLQARELPTSF*EMGLLQIDNVVSSGKACTWN AFRQQEVHLAEVQVQGRVR*QAGDPR*RTGVTG VVAQVWMFGGGGVLSSGEQLQMPVKPERGLGPS DGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSIRS MLLLTRSPTAWHRLSQLKPRVLPGTLGGQALHL RSWLLSRQGPAETGGQGQPQGPGLRTRLLITGL FGAGLGGAWLALRAEKERLQQQKRTEALRQAAVG QGDFHLLDHRGRARCKADFRGQWVLMYFGFTHC PDICPDELEKLVQVVRQLEAEPGLPPVQPVFIT VDPERDDVEAMARYVQDFHPRLLGLTGSTKQVAQ ASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLNP DGLFTDYYGRSRSAEQISDSVRRHMAAFRSVLS *ATAVWAPSLNGLRL

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQAADPGRVRHPRRPGAMA ALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQ LPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGA QIGAMLMAIRLRGMDLEETSVLTQALAQSGQQL EWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAA CGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQS PEQMQVLLDQAGCCIVGQSEQLVPADGILYAAR DVTATVDSLPLITASILSKKLVEGLSALVVDVK FGGAAVFPNQEQARELAKTLVGVGASLGLRVAAA LTAMDKPLGRCVGHALEVEEALLCMDGAGPPDL RDLVTTLGGALLWLSGHAGTQAQGAARVAAALD DGSALGRFERMLAAQGVDPGLARALCSGSPAERR QLLPRAREQEELLAPADGTVELVRALPLALVLH ELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGT PWLRVHRDGPALSGPQSRALQEALVLSDRAPFAA PSPFAELVLPPQQ*SSFAAKPCQCLGGSGRIQG CGGGGRLDEHVTPGGLRLPRTR*EPVSGCARVR MRSSGDACALPVSVWRPLSLSRRGRAQGAGGRRF LSCLVH*RAAPPRGADAGAGRGGGSPEDSLDSG VVSQLRLLRGAGGAHPRARWRPERGWGARGRLP AGTGVLAAKHSYNALARRFEWSWNLSGHEGAGLA GEGTNPEPPGRGCSESSGGCPQQEGAVTGSPET ST*QTFCGGPWERLKGHQRKGRASAVSPEELLG SLDVRRVGFCGGWKCRASLPGEVKHSRRAESELR G*VVQVFCWECGGRPRLVEVAGGNRKVLEESVA LGDCKPESYPPPFKKWVYCK*ITWLVQARHALG MLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE*QV WLPRCGCLVEVEF*AQVSSCKCLLSLNVGWVLQ MGGWSQVAGAAQALSWAFPSGS*RLCLFPGASD PCCC*LGAPQLGTGSLSSSLGSSLGPWEARPCI* GPGFCQGRALQRQVGRASPRALGFEPGC*SQAC SGLDSVGPGWP*GLRRRGCSSKSEQKPCARQLW ARATSTCWITEAGLAARLTSGASGC*CTLASLTA LTSAQTSWRSWCRWCGSWKQSLVCLQCSLSSSL WTPSGTTLKPWPATSRTSTQDCWV*PAPPNRLP RLVTVTACTTMQAPRMRTRTTSWTTPLPSTCSTL TASSRITTAGADRLSRSQTVCGGTWRLSAVSCL EPLQSGPHH*TGCV*

Sequence ECGF1.oAug05

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVD KHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPPRPYQGFS PPCSP

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFPD GSLYRTCSNQSIHRQLPTSSLAQSLQDPTKASP HPAP

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRGHADGHPTS GHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKASKTLPRLLP TLLP

Sequence ECGF1.gAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGC GCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGC CGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTC GGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGA CAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGT CTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGG GCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCAT CACAGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGT GGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCG GGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATC CCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGG TGAGCGTCGGGGGAGTCCCCGTCCTTCCGCCTCCGCCATCCCCTTCCCTTCCCGAGGCCCCGCCCCTTCCCGAGCCCGCGCCTCTCAGCCCCTCTCCCCG CAGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGT GGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGC CGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCT TTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCG CCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCG CTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACG CCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAG GGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGG CGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCT CGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCA CCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCG TCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGT TGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGT TTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCA GGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGG TGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGC CTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGC TCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGG CCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAG AGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGG CTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCA GCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGAC TTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGG ACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGAT CTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVD KHSTGGVGDKVSLVLAPALAACGCKVPMISGRG LGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQA GCCIVGQSEQLVPADGILYAARDVTATVDSLPLI TGWRGSQPRASGRGSADRHGQAPGSLRGPRPGG GGGAALHGRRRPARLKGPGHHARGRPALAQRTR GDSGPGRCPGGRGAGRRLGPWPLRADAGGAGRGS RSGPSPVLGKSRRTPAAAASRPGAGGAAGARRW *ASGESPSFRLRHPLPFPRPRPFPSPRLSAPLP AGTVELVRALPLALVLHELGAGRSRAGEPLRLGV GAELLVDVGQRLRRGTPWLRVHRDGPALSGPQS RALQEALVLSDRAPFAAPSPFAELVLPPQQ*SS FAAKPCQCLGGSGRIQGCGGGGRLDEHVTPGGLR LPRTR*EPVSGCARVRMRSSGDACALPVSVWRP LSLSRRGRAQGAGGRRFLSCLVH*RAAPPRGAD AGAGRGGGSPEDSLDSGVVSQLRLLRGAGGAHPR ARWRPERGWGARGRLPAGTGVLAAKHSYNALAR RFEWSWNLSGHEGAGLAGEGTNPEPPGRGCSES SGGCPQQEGAVTGSPETST*QTFCGGPWERLKGH QRKGRASAVSPEELLGSLDVRRVGFCGGWKCRA SLPGEVKHSRRAESELRG*VVQVFCWECGGRPR LVEVAGGNRKVLEESVALGDCKPESYPPPFKKWV YCK*ITWLVQARHALGMLSVSKRFTLLRCRCRA GCGDRLVIPGRGQE*QVWLPRCGCLVEVEF*AQ VSSCKCLLSLNVGWVLQMGGWSQVAGAAQALSWA FPSGS*RLCLFPGASDPCCC*LGAPQLGTGSLS SSLGSSLGPWEARPCI*GPGFCQGRALQRQVGR ASPRALGFEPGC*SQACSGLDSVGPGWP*GLRRR GCSSKSEQKPCARQLWARATSTCWITEAGLAAR LTSGASGC*CTLASLTALTSAQTSWRSWCRWCG SWKQSLVCLQCSLSSSLWTPSGTTLKPWPATSRT STQDCWV*PAPPNRLPRLVTVTACTTMQAPRMR TRTTSWTTPLPSTCSTLTASSRITTAGADRLSR SQTVCGGTWRLSAVSCLEPLQSGPHH*TGCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARCQ*SADVV WGTQEAPWISWSLFLDSMSSRAQSRCKCCWTRR AAVSWVRVSSWFLRTESYMQPEM*QPPWTACHS SQVGVGASLGLRVAAALTAMDKPLGRCVGHALEV EEALLCMDGAGPPDLRDLVTTLGGALLWLSGHA GTQAQGAARVAAALDDGSALGRFERMLAAQGVD PGLARALCSGSPAERRQLLPRAREQEELLAPADG ERRGSPRPSASAIPFPSRGPAPSRARASQPLSP QAPWSWSGRCRWRWCCTSSGPGAAALGSRSAWG WAQSCWSTWVRGCAVGPPGSACTGTAPRSAARRA APCRRRSYSPTARHSPPPRPSQSSFCRRSNKAP LPRNLVSAWAGAEGSRAAEAGAVSMNT*PPAGS AFRARAESLSAAAPVCACAAPGTPAPCL*ACGAR FP*AGGAERRELEVGASSRAWSTDARPRREVRT PGLGGEEVALRTRWTPG*FPSSGYCAGLAGHTP GRAGGRSEAGAPVGGSQQAPVFSRPSTVITRSRG ASSGPGTFLATRALALLGRAQTQNRPGGGAVSP REGALSRRGQ*PGVLRPPPSKPSAGARGKGSKV TNAKAGRRL*ARRSCWEAWM*GGWGFVAGGSVVR LCQERLNTAGGQSLSSGGRSCRFSAGSVEEGRG WLKWLEVTGKCWRNRLLSGIASQRVTHLLLRNG FIANR*RG*FRQGMHLECFPSARGSPC*GAGAGQ GAVTGW*SQVEDRSDRCGCPGVDVWWRWSSELR *AAANAC*A*TWAGSFRWVAGLKSQGQPRHCPG PSLLAPDACACFQEHQIHAAADSEPHSLAQALSA QASGPPWDPGRPGPASEVLAFVKAGPCRDRWAG PAPGPWASNPAADHRPVRGWTRWGLAGPEG*EG EAAAAKANRSPAPGSCGPGRLPPAGSQRPGSLQG *LPGPVGADVLWLHSLP*HLPRRAGEAGAGGAA AGSRAWFASSAACLHHCGPRAGRR*SHGPLRPG LPPKTVGSDRLHQTGCPG*SQLPRVLQCRPQG*G PGLHRGPLHCHLPAQP*RPLHGLLRPEQIG*AD LRQCAAAHGGFPQCPVLSHCSLGPIIKRAAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRGHADGHPTS GHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQGANDQRTW SGAHRRHLG*AGVYSWIQCHPEPRADASAAGPGG LLYRGSE*AAGSCGRNPICSQRCDSHRGQPATH HRLAWEPA*GFGSRQR*PPWTSPWVAAWATPWR WRRRCSAWTAQARQT*GTWSPRSGAPCSGSADTR GLRPRALPGWPRRWTTARPLAASSGCWRRRAWI PVWPEPCAREVPQNAGSCCLAPGSRRSCWRPQM VSVGGVPVLPPPPSPSLPEAPPLPEPAPLSPSPR RHRGAGPGAAAGAGAARARGRAQPRWGAAPPGG GRRAAGRRGSEAAPWDPLAPRAPGRPRAQRPAE PRPAGGARTLRPRAIRRPLALRRARSAAAAIKLL CRETLSVLGRERKDPGLRRRGPSR*TRDPRRAP PSAHALRACQRLRPCAHAQLRGRLRPACERVAP AFPEPAGQSAGSWRSALPLVLGPLTRGPAARCGR RGWEGRR*P*GLAGLRGSFPAPATARGWRGTPQ GALEAGARLGRPWEAPSRHRCSRGQAQL*RARA ALRVVLEPFWPRGRWPCWGGHKPRTARAGVQ*VL GRVPSAGGGSDRES*DLHLANLLRGPVGKAQRS PTQRQGVGCEPGGAAGKPGCEEGGVLWRVEVSC VSARRG*TQPAGRV*APGVGRAGFLLGVWRKAAV G*SGWR*QESVGGIGCSRGLQARELPTSF*EMG LLQIDNVVSSGKACTWNAFRQQEVHLAEVQVQG RVR*QAGDPR*RTGVTGVVAQVWMFGGGGVLSSG EQLQMPVKPERGLGPSDGWLVSSRRGSPGTVLG LPFWLLTPVLVSRSIRSMLLLTRSPTAWHRLSQ LKPRVLPGTLGGQALHLRSWLLSRQGPAETGGQG QPQGPGLRTRLLITGLFGAGLGGAWLALRAEKE RLQQQKRTEALRQAAVGQGDFHLLDHRGRARCK ADFRGQWVLMYFGFTHCPDICPDELEKLVQVVRQ LEAEPGLPPVQPVFITVDPERDDVEAMARYVQD FHPRLLGLTGSTKQVAQASHSYRVYYNAGPKDE DQDYIVDHSIAIYLLNPDGLFTDYYGRSRSAEQI SDSVRRHMAAFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.eAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGC GCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGC CGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTC GGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGA CAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGATGCAAGTGCTGCTGGACCAGGCG GGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCA TCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCA GGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGC GTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGC TCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCT GGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAG CTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGC CGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCT CAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCG CAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGA CCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGT GAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCG CCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTG GCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTA TAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGG GGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGG AAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGG GTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGT GGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTC CTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAG GTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTG GAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCC AGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGG CACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAG AGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCT GAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGC CGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGG TGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGC CCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCA GGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCA GATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGG GCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVD KHSTGGVGDKVSLVLAPALAACGCKMQVLLDQA GCCIVGQSEQLVPADGILYAARDVTATVDSLPL ITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQ ARELAKTLVGVGASLGLRVAAALTAMDKPLGRC VGHALEVEEALLCMDGAGPPDLRDLVTTLGGAL LWLSGHAGTQAQGAARVAAALDDGSALGRFERML AAQGVDPGLARALCSGSPAERRQLLPRAREQEE LLAPADGTVELVRALPLALVLHELGAGRSRAGE PLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPAL SGPQSRALQEALVLSDRAPFAAPSPFAELVLPP QQ*SSFAAKPCQCLGGSGRIQGCGGGGRLDEHV TPGGLRLPRTR*EPVSGCARVRMRSSGDACALPV SVWRPLSLSRRGRAQGAGGRRFLSCLVH*RAAP PRGADAGAGRGGGSPEDSLDSGVVSQLRLLRGA GGAHPRARWRPERGWGARGRLPAGTGVLAAKHSY NALARRFEWSWNLSGHEGAGLAGEGTNPEPPGR GCSESSGGCPQQEGAVTGSPETST*QTFCGGPW ERLKGHQRKGRASAVSPEELLGSLDVRRVGFCGG WKCRASLPGEVKHSRRAESELRG*VVQVFCWEC GGRPRLVEVAGGNRKVLEESVALGDCKPESYPP PFKKWVYCK*ITWLVQARHALGMLSVSKRFTLLR CRCRAGCGDRLVIPGRGQE*QVWLPRCGCLVEV EF*AQVSSCKCLLSLNVGWVLQMGGWSQVAGAA QALSWAFPSGS*RLCLFPGASDPCCC*LGAPQLG TGSLSSSLGSSLGPWEARPCI*GPGFCQGRALQ RQVGRASPRALGFEPGC*SQACSGLDSVGPGWP *GLRRRGCSSKSEQKPCARQLWARATSTCWITEA GLAARLTSGASGC*CTLASLTALTSAQTSWRSW CRWCGSWKQSLVCLQCSLSSSLWTPSGTTLKPW PATSRTSTQDCWV*PAPPNRLPRLVTVTACTTMQ APRMRTRTTSWTTPLPSTCSTLTASSRITTAGA DRLSRSQTVCGGTWRLSAVSCLEPLQSGPHH*T GCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARCKCCWTRR AAVSWVRVSSWFLRTESYMQPEM*QPPWTACHS SQPPFSVRNSWRGCPLWWWTLSSEGPPSSPTRS RPGSWQRRWLAWEPA*GFGSRQR*PPWTSPWVAA WATPWRWRRRCSAWTAQARQT*GTWSPRSGAPC SGSADTRGLRPRALPGWPRRWTTARPLAASSGC WRRRAWIPVWPEPCAREVPQNAGSCCLAPGSRRS CWRPQMAPWSWSGRCRWRWCCTSSGPGAAALGS RSAWGWAQSCWSTWVRGCAVGPPGSACTGTAPR SAARRAAPCRRRSYSPTARHSPPPRPSQSSFCRR SNKAPLPRNLVSAWAGAEGSRAAEAGAVSMNT* PPAGSAFRARAESLSAAAPVCACAAPGTPAPCL *ACGARFP*AGGAERRELEVGASSRAWSTDARPR REVRTPGLGGEEVALRTRWTPG*FPSSGYCAGL AGHTPGRAGGRSEAGAPVGGSQQAPVFSRPSTV ITRSRGASSGPGTFLATRALALLGRAQTQNRPGG GAVSPREGALSRRGQ*PGVLRPPPSKPSAGARG KGSKVTNAKAGRRL*ARRSCWEAWM*GGWGFVA GGSVVRLCQERLNTAGGQSLSSGGRSCRFSAGSV EEGRGWLKWLEVTGKCWRNRLLSGIASQRVTHL LLRNGFIANR*RG*FRQGMHLECFPSARGSPC* GAGAGQGAVTGW*SQVEDRSDRCGCPGVDVWWRW SSELR*AAANAC*A*TWAGSFRWVAGLKSQGQP RHCPGPSLLAPDACACFQEHQIHAAADSEPHSL AQALSAQASGPPWDPGRPGPASEVLAFVKAGPCR DRWAGPAPGPWASNPAADHRPVRGWTRWGLAGP EG*EGEAAAAKANRSPAPGSCGPGRLPPAGSQR PGSLQG*LPGPVGADVLWLHSLP*HLPRRAGEAG AGGAAAGSRAWFASSAACLHHCGPRAGRR*SHG PLRPGLPPKTVGSDRLHQTGCPG*SQLPRVLQC RPQG*GPGLHRGPLHCHLPAQP*RPLHGLLRPEQ IG*ADLRQCAAAHGGFPQCPVLSHCSLGPIIKR AAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRGHADGHPTS GHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQDASAAGPG GLLYRGSE*AAGSCGRNPICSQRCDSHRGQPATH HSLHSQ*ETRGGAVRSGGGR*VRRGRRLPQPGA GPGAGKDAGWRGSQPRASGRGSADRHGQAPGSL RGPRPGGGGGAALHGRRRPARLKGPGHHARGRPA LAQRTRGDSGPGRCPGGRGAGRRLGPWPLRADA GGAGRGSRSGPSPVLGKSRRTPAAAASRPGAGG AAGARRWHRGAGPGAAAGAGAARARGRAQPRWGA APPGGGRRAAGRRGSEAAPWDPLAPRAPGRPRA QRPAEPRPAGGARTLRPRAIRRPLALRRARSAA AAIKLLCRETLSVLGRERKDPGLRRRGPSR*TRD PRRAPPSAHALRACQRLRPCAHAQLRGRLRPAC ERVAPAFPEPAGQSAGSWRSALPLVLGPLTRGP AARCGRRGWEGRR*P*GLAGLRGSFPAPATARGW RGTPQGALEAGARLGRPWEAPSRHRCSRGQAQL *RARAALRVVLEPFWPRGRWPCWGGHKPRTARA GVQ*VLGRVPSAGGGSDRES*DLHLANLLRGPVG KAQRSPTQRQGVGCEPGGAAGKPGCEEGGVLWR VEVSCVSARRG*TQPAGRV*APGVGRAGFLLGV WRKAAVG*SGWR*QESVGGIGCSRGLQARELPTS F*EMGLLQIDNVVSSGKACTWNAFRQQEVHLAE VQVQGRVR*QAGDPR*RTGVTGVVAQVWMFGGG GVLSSGEQLQMPVKPERGLGPSDGWLVSSRRGSP GTVLGLPFWLLTPVLVSRSIRSMLLLTRSPTAW HRLSQLKPRVLPGTLGGQALHLRSWLLSRQGPA ETGGQGQPQGPGLRTRLLITGLFGAGLGGAWLAL RAEKERLQQQKRTEALRQAAVGQGDFHLLDHRG RARCKADFRGQWVLMYFGFTHCPDICPDELEKL VQVVRQLEAEPGLPPVQPVFITVDPERDDVEAMA RYVQDFHPRLLGLTGSTKQVAQASHSYRVYYNA GPKDEDQDYIVDHSIAIYLLNPDGLFTDYYGRS RSAEQISDSVRRHMAAFRSVLS*ATAVWAPSLNG LRL

Sequence ECGF1.dAug05

TTCTTTTTTTTACATTTTGTTTGCCTAGAATTGTATTTGATTGTAATCAAATCATGTGTCACATCTTTTTTTCAATTTGTGTGATTATTTTGTGGCCCAA TACATGATTTTTTTTGCGAATATTTCATGGACTTAAATAGATAAGTCCTCTCTCTACACATAATTAAATGGAGTGTGTGCTTTGCATTATTCAGTTCCTT TATTCCCATCCTTGCTTTTGTCTGCAGATTTGTCCTATTCCAGAATGGGTATACTGAAACTTGGGTAAGGGTGAGGCCAGCGTTCTGGGTGGCCCAGGCT ACTATCCCACCCGTGCCACAGAATCCACACAGAGGCCCAGCGGCGGCACTGGGTGGTGGGGAAGGGGGAGGCGGACCCAGGTTGGGGGGGCAGGCAGTCA GTGCGGTTGCCAGGAAGCGGGAAAGGGCCACAATGGGGTCTGGGAGGTGGGCGGGGCGGAGCGGGGATGTCCAGCCACGTCGCTTTGTTTTCCCACGCTA GGAGCTACCACAACAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGG CCCCATCGCGGCGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCC TTCACCTTCGGCGCGCGCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCG CCCCCGCCTACTCCATCTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTA CCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGGTGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTG GGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCCATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGACCCCGGGCC CCTGCGCCTATCAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGGCGCGGACTTCGCTCCCCCAAGACAACACTCGGAA GCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCGGCACTCGGACTACCTGGCCCCGCTGGTGACCGAC GCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCATCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGT CCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCGGGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGC GCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGACGCCCGAGACCCACGCCGGGCGGGACCGGCCAGGG ATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGGGATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGG GGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAAC CCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCCGGAGCGAT GGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAG CAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGG GCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCA GCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCG GCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCC AGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGC CAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTT AAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGG CAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGA CTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTG GACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAAC GCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCT GCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGG ACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCG CCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCA GGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTG CGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGC GCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTC CGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTC CCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCC TTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGA GACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTG GGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGC TCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGT TGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTT GGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGAC AGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCT TCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCA GATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCA TCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGC CTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTG TGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCA CTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATC ACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTG CCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAA CCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTG TCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

FFFLHFVCLELYLIVIKSCVTSFFQFV*LFCGP IHDFFCEYFMDLNR*VLSLHIIKWSVCFALFSSF IPILAFVCRFVLFQNGYTETWVRVRPAFWVAQA TIPPVPQNPHRGPAAALGGGEGGGGPRLGGQAV SAVARKRERATMGSGRWAGRSGDVQPRRFVFPR* ELPQQVLRAVSAPHPRYGLGRLGGPLAATPAPR PHRGALRRPRAQIQAAAQHRLRPA*PVAAARPR LHLRRALPHAADDVRPRARPPGARSHDRARHRRR PRLLHLRPPTPLSALPHSGTWQVLPGASGERDV PQCASAHHCSPKLGCPGGTAEPRSRGLYGALAL GSARHRQSLRPNLLHLRPQSGWQFLRGPQQDPGP LRLSGRESRGLQVPGPPVHDSGADFAPPRQHSE ARARGLQRGSAPEAPRLEFRDPALGLPGPAGDR RGQLTRQAGAAPHVFA*SLRVRIVSASLSLSLRV LAQGLGWSHGWGRVPTPNPAGRGPSVGRGRDPS AAPRPNVETPPRAGGGLSGATDARDPRRAGPAR DHPRRRPRAPTARKFRVSGGTGGLAGARRARLPG GGDCRAPPSRRPHPPAVLGRRRPAAGSGPLCAN PEPYGPDPRARPGTWAGIRSKRARAAP*AGPER WQP**PREPGPHPRLVTSPGKGARDFPTLRQSPS SSRS*SA*SETEAA*AKRTSGASWPLW*MGARR AHRSGPC*WPSDFGAWIWRRPRC*PRPWLSRDS SWSGQRPGASSLWTSIPQGVWVTRSAWSSHLPWR HVAARCQ*SADVVWGTQEAPWISWSLFLDSMSS RAQSRCKCCWTRRAAVSWVRVSSWFLRTESYMQ PEM*QPPWTACHSSQPPFSVRNSWRGCPLWWWTL SSEGPPSSPTRSRPGSWQRRWLAWEPA*GFGSR QR*PPWTSPWVAAWATPWRWRRRCSAWTAQARQ T*GTWSPRSGAPCSGSADTRGLRPRALPGWPRRW TTARPLAASSGCWRRRAWIPVWPEPCAREVPQN AGSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWC CTSSGPGAAALGSRSAWGWAQSCWSTWVRGCAVG PPGSACTGTAPRSAARRAAPCRRRSYSPTARHS PPPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGS RAAEAGAVSMNT*PPAGSAFRARAESLSAAAPVC ACAAPGTPAPCL*ACGARFP*AGGAERRELEVG ASSRAWSTDARPRREVRTPGLGGEEVALRTRWT PG*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGGS QQAPVFSRPSTVITRSRGASSGPGTFLATRALA LLGRAQTQNRPGGGAVSPREGALSRRGQ*PGVL RPPPSKPSAGARGKGSKVTNAKAGRRL*ARRSCW EAWM*GGWGFVAGGSVVRLCQERLNTAGGQSLS SGGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNR LLSGIASQRVTHLLLRNGFIANR*RG*FRQGMHL ECFPSARGSPC*GAGAGQGAVTGW*SQVEDRSD RCGCPGVDVWWRWSSELR*AAANAC*A*TWAGS FRWVAGLKSQGQPRHCPGPSLLAPDACACFQEHQ IHAAADSEPHSLAQALSAQASGPPWDPGRPGPA SEVLAFVKAGPCRDRWAGPAPGPWASNPAADHR PVRGWTRWGLAGPEG*EGEAAAAKANRSPAPGSC GPGRLPPAGSQRPGSLQG*LPGPVGADVLWLHS LP*HLPRRAGEAGAGGAAAGSRAWFASSAACLH HCGPRAGRR*SHGPLRPGLPPKTVGSDRLHQTGC PG*SQLPRVLQCRPQG*GPGLHRGPLHCHLPAQ P*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCP VLSHCSLGPIIKRAAF

Frame 1

SFFYILFA*NCI*L*SNHVSHLFFNLCDYFVAQ YMIFFANISWT*IDKSSLYT*LNGVCALHYSVP LFPSLLLSADLSYSRMGILKLG*G*GQRSGWPRL LSHPCHRIHTEAQRRHWVVGKGEADPGWGGRQS VRLPGSGKGPQWGLGGGRGGAGMSSHVALFSHA RSYHNRCCEP*APPTRAMGSDAWVGLWRPHRPRG PIAAHYGGPGPKYKLPPNTGYALHDPSRPRAPA FTFGARFPTQQTTCGPGPGHLVPARMTVRGTDG APAYSIYGRPRRSAPFLTPGPGRYFPERAGNATY PSAPRHTIAPRNWGVQAEQQSPGPAAYTVPSLL GPRVIGKVSAPTCSIYGRRAAGSFFEDLSKTPG PCAYQVVSPGVYKSRAPQFTILARTSLPQDNTRK PGPAAYNVDQHRKPRGWSFGIRHSDYLAPLVTD ADN*PARRERPHTCLLKVCESASCPPLSLSLCA SWRKAWGGATAGAVSQLRTQRGGARASGEAGTPA LRRVRTSRPHRGREGDSREPQTPETHAGRDRPG ITPADGPGPRRPGSSACPGAPGDWPGRGVQGFP GAATAELRPPGGPTRLPSWGAAAPPPAVDRCART LNPTVPTRGRGRVPGLGSGASGRGQRPKQARSD GSLDDPGNRGPTRAW*LLRGREPGTSRPFARAQ AAPGADPHEARRRPPERSGHQGLRGRCGEWERAG RTDRGHADGHPTSGHGSGGDLGADPGPGSVGTA AGVARGLAPAACGQAFHRGCG*QGQPGPRTCPG GMWLQGANDQRTWSGAHRRHLG*AGVYSWIQCHP EPRADASAAGPGGLLYRGSE*AAGSCGRNPICS QRCDSHRGQPATHHSLHSQ*ETRGGAVRSGGGR *VRRGRRLPQPGAGPGAGKDAGWRGSQPRASGRG SADRHGQAPGSLRGPRPGGGGGAALHGRRRPAR LKGPGHHARGRPALAQRTRGDSGPGRCPGGRGA GRRLGPWPLRADAGGAGRGSRSGPSPVLGKSRRT PAAAASRPGAGGAAGARRWHRGAGPGAAAGAGA ARARGRAQPRWGAAPPGGGRRAAGRRGSEAAPW DPLAPRAPGRPRAQRPAEPRPAGGARTLRPRAIR RPLALRRARSAAAAIKLLCRETLSVLGRERKDP GLRRRGPSR*TRDPRRAPPSAHALRACQRLRPC AHAQLRGRLRPACERVAPAFPEPAGQSAGSWRSA LPLVLGPLTRGPAARCGRRGWEGRR*P*GLAGL RGSFPAPATARGWRGTPQGALEAGARLGRPWEA PSRHRCSRGQAQL*RARAALRVVLEPFWPRGRWP CWGGHKPRTARAGVQ*VLGRVPSAGGGSDRES* DLHLANLLRGPVGKAQRSPTQRQGVGCEPGGAA GKPGCEEGGVLWRVEVSCVSARRG*TQPAGRV*A PGVGRAGFLLGVWRKAAVG*SGWR*QESVGGIG CSRGLQARELPTSF*EMGLLQIDNVVSSGKACT WNAFRQQEVHLAEVQVQGRVR*QAGDPR*RTGVT GVVAQVWMFGGGGVLSSGEQLQMPVKPERGLGP SDGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSI RSMLLLTRSPTAWHRLSQLKPRVLPGTLGGQALH LRSWLLSRQGPAETGGQGQPQGPGLRTRLLITG LFGAGLGGAWLALRAEKERLQQQKRTEALRQAA VGQGDFHLLDHRGRARCKADFRGQWVLMYFGFTH CPDICPDELEKLVQVVRQLEAEPGLPPVQPVFI TVDPERDDVEAMARYVQDFHPRLLGLTGSTKQV AQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLN PDGLFTDYYGRSRSAEQISDSVRRHMAAFRSVL S*ATAVWAPSLNGLRL

Frame 2

LFFTFCLPRIVFDCNQIMCHIFFSICVIILWPN T*FFLRIFHGLK*ISPLSTHN*MECVLCIIQFL YSHPCFCLQICPIPEWVY*NLGKGEASVLGGPG YYPTRATESTQRPSGGTGWWGRGRRTQVGGAGSQ CGCQEAGKGHNGVWEVGGAERGCPATSLCFPTL GATTTGAASRKRPPPALWARTPGWAFGGHTGPA APSRRTTEAPGPNTSCRPTPATPCMTRRGRAPPP SPSARASPRSRRRAAPGQATWCPLA*PCAAPTA PPPTPSTAAHAAQRPSSLRDLAGTSRSERGTRR TPVRLGTPLLPETGVSRRNSRAQVPRPIRCPRSW VRASSAKSPPQLAPSTAAERLAVSSRTSARPRA PAPIRS*VQGSTSPGPPSSRFWRGLRSPKTTLG SQGPRPTTWISTGSPAAGVSGSGTRTTWPRW*PT RTTDPPGGSGPTRVCLKSASPHRVRLSLSLSAR PGARPGVEPRLGPCPNSEPSGAGPERRARPGPQ RCAASERRDPTEGGRGTLGSHRRPRPTPGGTGQG SPPPTAPGPDGPEVPRVRGHRGIGRGAACKASR GRRLPSSALQAAPPACRPGAPPPRRRQWTAVRE P*TLRSRPAGEAGYLGWDPEQAGEGSALSRPGAM AALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPK QLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQ GAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQ LEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALA ACGCKVPMISGRGLGHTGGTLDKLESIPGFNVI QSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAA RDVTATVDSLPLITASILSKKLVEGLSALVVDV KFGGAAVFPNQEQARELAKTLVGVGASLGLRVA AALTAMDKPLGRCVGHALEVEEALLCMDGAGPPD LRDLVTTLGGALLWLSGHAGTQAQGAARVAAAL DDGSALGRFERMLAAQGVDPGLARALCSGSPAE RRQLLPRAREQEELLAPADGTVELVRALPLALVL HELGAGRSRAGEPLRLGVGAELLVDVGQRLRRG TPWLRVHRDGPALSGPQSRALQEALVLSDRAPF AAPSPFAELVLPPQQ*SSFAAKPCQCLGGSGRIQ GCGGGGRLDEHVTPGGLRLPRTR*EPVSGCARV RMRSSGDACALPVSVWRPLSLSRRGRAQGAGGR RFLSCLVH*RAAPPRGADAGAGRGGGSPEDSLDS GVVSQLRLLRGAGGAHPRARWRPERGWGARGRL PAGTGVLAAKHSYNALARRFEWSWNLSGHEGAG LAGEGTNPEPPGRGCSESSGGCPQQEGAVTGSPE TST*QTFCGGPWERLKGHQRKGRASAVSPEELL GSLDVRRVGFCGGWKCRASLPGEVKHSRRAESE LRG*VVQVFCWECGGRPRLVEVAGGNRKVLEESV ALGDCKPESYPPPFKKWVYCK*ITWLVQARHAL GMLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE* QVWLPRCGCLVEVEF*AQVSSCKCLLSLNVGWVL QMGGWSQVAGAAQALSWAFPSGS*RLCLFPGAS DPCCC*LGAPQLGTGSLSSSLGSSLGPWEARPC I*GPGFCQGRALQRQVGRASPRALGFEPGC*SQA CSGLDSVGPGWP*GLRRRGCSSKSEQKPCARQL WARATSTCWITEAGLAARLTSGASGC*CTLASL TALTSAQTSWRSWCRWCGSWKQSLVCLQCSLSSS LWTPSGTTLKPWPATSRTSTQDCWV*PAPPNRL PRLVTVTACTTMQAPRMRTRTTSWTTPLPSTCS TLTASSRITTAGADRLSRSQTVCGGTWRLSAVSC LEPLQSGPHH*TGCV*

Sequence ECGF1.aAug05

AACAGCACGAAAATCTCCTGCCCCCACCCCACCCCCAGGTGATTCCCTTCACCAAGTCTGGGTCTAGACTTGGCCCCCGCGAGTTCACGTCCCTGCCTAA TGCGCCTGGAGTCCGGGTGATGGCCCTGGATCCGCTCCCGGCCCCAAGCTCCACACAGCCGGGAAGGAAGCTCGCGTTTGTTTTGCTGGCGCCAGGGATA CGGTCCAGGCCCCGCCCCGCCCCGCGCTGATCCCAGGGTAGTGGGAAAGTGCGCTCCCCAAGAATTTGCGGTGCAGGGGCCGCGGCCACCGCCTTCTGTC CGCAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGGCCCCATCGCGG CGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGTAGAAGGGGCAGGCCCCGGGATTGAGGGGTGGGACGGCGCCTGGGGGAGGG AAGGGGAAGAGACCAGGGAGGCCTGCGCTCGGGGCTGAACCGTGCCTGCTAGAGGGGAGAGGGGGTCTGTGCCGGGGGAGGGTCCGTGTTGCAGATGGGG CTTGGGGCTGGGCCCGGCCAGACGCTAACTCGGATGCTCCCAGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCCTTCACCTTCGGCGCGC GCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCGCCCCCGCCTACTCCAT CTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGTCAGGACCCCCGGGCCCCTGGCCACCCCAACGCCGAACTGCCTCCAGGGAGG CCCACCTGGGAACCCCCGACCTGAACCCCGAGTCCCCCTCGGATACCCTAACACCGCATATTCGGTACCCCCATATCCGGATCTCAAATCCCAAACCCCG AACCCCACGGGGCTTTGATAAATCGTGGCTCAGACTCCCCACTAGTCCCAGGACCCCATCTCGGGTACCCACCAGGCTCCCACGCAGTTCTAGCCCCCCA CACCCTTGATCCGCCCCGCAGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTACCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGG TGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTGGGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCC ATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGG CGCGGACTTCGCTCCCCCAAGACAACACTCGGAAGCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCG GCACTCGGACTACCTGGCCCCGCTGGTGACCGACGCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCA TCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGTCCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCG GGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGCGCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGAC GCCCGAGACCCACGCCGGGCGGGACCGGCCAGGGATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGG GATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGGGGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCG CCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGG GCGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAG GGGCGGACACCGGAGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCC CCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGAC CCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGG TGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCT GGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTC CTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTC CTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGA CGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCC GCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCC TAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGA CGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCC CGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCT CGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCT GCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGT CAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCT CCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGG CGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTC AGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGC AGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCC TGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGG GGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTG GCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGC AGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTG AGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCC GGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAA AGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTT CAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAG GTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCT GAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGC TTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCT GGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACC CGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAG CCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGAT GTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCA GTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCG GCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCAT TGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCG GCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

NSTKISCPHPTPR*FPSPSLGLDLAPASSRPCL MRLESG*WPWIRSRPQAPHSREGSSRLFCWRQGY GPGPAPPRADPRVVGKCAPQEFAVQGPRPPPSV RRCCEP*APPTRAMGSDAWVGLWRPHRPRGPIA AHYGGPGPKYKLPPNTGRRGRPRD*GVGRRLGEG RGRDQGGLRSGLNRAC*RGEGVCAGGGSVLQMG LGAGPGQTLTRMLPGYALHDPSRPRAPAFTFGA RFPTQQTTCGPGPGHLVPARMTVRGTDGAPAYSI YGRPRRSAPFLTPGPGQDPRAPGHPNAELPPGR PTWEPPT*TPSPPRIP*HRIFGTPISGSQIPNP EPHGALINRGSDSPLVPGPHLGYPPGSHAVLAPH TLDPPRRQVLPGASGERDVPQCASAHHCSPKLG CPGGTAEPRSRGLYGALALGSARHRQSLRPNLL HLRPQSGWQFLRGPQQGRESRGLQVPGPPVHDSG ADFAPPRQHSEARARGLQRGSAPEAPRLEFRDP ALGLPGPAGDRRGQLTRQAGAAPHVFA*SLRVR IVSASLSLSLRVLAQGLGWSHGWGRVPTPNPAGR GPSVGRGRDPSAAPRPNVETPPRAGGGLSGATD ARDPRRAGPARDHPRRRPRAPTARKFRVSGGTG GLAGARRARLPGGGDCRAPPSRRPHPPAVLGRRR PAAGSGPLCANPEPYGPDPRARPGTWAGIRSKR ARAAP*AGTGGAQVARRGSGRQTRTRATQTRDR GADTGETRERGRDRSTWLRHRRREAADPGRVRHP RRPGAMAALMTPGTGAPPAPGDFSGEGSQGLPD PSPEPKQLPELIRMKRDGGRLSEADIRGFVAAV VNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVPMISGRGLGHTGGTLDKLESI PGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAD GILYAARDVTATVDSLPLITASILSKKLVEGLS ALVVDVKFGGAAVFPNQEQARELAKTLVGVGAS LGLRVAAALTAMDKPLGRCVGHALEVEEALLCMD GAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAA RVAAALDDGSALGRFERMLAAQGVDPGLARALC SGSPAERRQLLPRAREQEELLAPADGTVELVRAL PLALVLHELGAGRSRAGEPLRLGVGAELLVDVG QRLRRGTPWLRVHRDGPALSGPQSRALQEALVL SDRAPFAAPSPFAELVLPPQQ*SSFAAKPCQCLG GSGRIQGCGGGGRLDEHVTPGGLRLPRTR*EPV SGCARVRMRSSGDACALPVSVWRPLSLSRRGRA QGAGGRRFLSCLVH*RAAPPRGADAGAGRGGGSP EDSLDSGVVSQLRLLRGAGGAHPRARWRPERGW GARGRLPAGTGVLAAKHSYNALARRFEWSWNLS GHEGAGLAGEGTNPEPPGRGCSESSGGCPQQEGA VTGSPETST*QTFCGGPWERLKGHQRKGRASAV SPEELLGSLDVRRVGFCGGWKCRASLPGEVKHS RRAESELRG*VVQVFCWECGGRPRLVEVAGGNRK VLEESVALGDCKPESYPPPFKKWVYCK*ITWLV QARHALGMLSVSKRFTLLRCRCRAGCGDRLVIP GRGQE*QVWLPRCGCLVEVEF*AQVSSCKCLLSL NVGWVLQMGGWSQVAGAAQALSWAFPSGS*RLC LFPGASDPCCC*LGAPQLGTGSLSSSLGSSLGP WEARPCI*GPGFCQGRALQRQVGRASPRALGFEP GC*SQACSGLDSVGPGWP*GLRRRGCSSKSEQK PCARQLWARATSTCWITEAGLAARLTSGASGC* CTLASLTALTSAQTSWRSWCRWCGSWKQSLVCLQ CSLSSSLWTPSGTTLKPWPATSRTSTQDCWV*P APPNRLPRLVTVTACTTMQAPRMRTRTTSWTTP LPSTCSTLTASSRITTAGADRLSRSQTVCGGTWR LSAVSCLEPLQSGPHH*TGCV*

Frame 1

TARKSPAPTPPPGDSLHQVWV*TWPPRVHVPA* CAWSPGDGPGSAPGPKLHTAGKEARVCFAGARD TVQAPPRPALIPG*WESALPKNLRCRGRGHRLLS AGAASRKRPPPALWARTPGWAFGGHTGPAAPSR RTTEAPGPNTSCRPTPVEGAGPGIEGWDGAWGR EGEETREACARG*TVPARGERGSVPGEGPCCRWG LGLGPARR*LGCSQATPCMTRRGRAPPPSPSAR ASPRSRRRAAPGQATWCPLA*PCAAPTAPPPTP STAAHAAQRPSSLRDLVRTPGPLATPTPNCLQGG PPGNPRPEPRVPLGYPNTAYSVPPYPDLKSQTP NPTGL**IVAQTPH*SQDPISGTHQAPTQF*PP TPLIRPAGRYFPERAGNATYPSAPRHTIAPRNWG VQAEQQSPGPAAYTVPSLLGPRVIGKVSAPTCS IYGRRAAGSFFEDLSKVVSPGVYKSRAPQFTIL ARTSLPQDNTRKPGPAAYNVDQHRKPRGWSFGIR HSDYLAPLVTDADN*PARRERPHTCLLKVCESA SCPPLSLSLCASWRKAWGGATAGAVSQLRTQRG GARASGEAGTPALRRVRTSRPHRGREGDSREPQT PETHAGRDRPGITPADGPGPRRPGSSACPGAPG DWPGRGVQGFPGAATAELRPPGGPTRLPSWGAA APPPAVDRCARTLNPTVPTRGRGRVPGLGSGASG RGQRPKQVRAGLKSRGGEAGGRHGRGRHRHGTE GRTPERHGKGVGTGARGSDTDAGRPQTPDVSGI PAGPERWQP**PREPGPHPRLVTSPGKGARDFPT LRQSPSSSRS*SA*SETEAA*AKRTSGASWPLW *MGARRAHRSGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARCQ*SADVVWGTQEAPWISWSLF LDSMSSRAQSRCKCCWTRRAAVSWVRVSSWFLR TESYMQPEM*QPPWTACHSSQPPFSVRNSWRGCP LWWWTLSSEGPPSSPTRSRPGSWQRRWLAWEPA *GFGSRQR*PPWTSPWVAAWATPWRWRRRCSAW TAQARQT*GTWSPRSGAPCSGSADTRGLRPRALP GWPRRWTTARPLAASSGCWRRRAWIPVWPEPCA REVPQNAGSCCLAPGSRRSCWRPQMAPWSWSGR CRWRWCCTSSGPGAAALGSRSAWGWAQSCWSTWV RGCAVGPPGSACTGTAPRSAARRAAPCRRRSYS PTARHSPPPRPSQSSFCRRSNKAPLPRNLVSAW AGAEGSRAAEAGAVSMNT*PPAGSAFRARAESLS AAAPVCACAAPGTPAPCL*ACGARFP*AGGAER RELEVGASSRAWSTDARPRREVRTPGLGGEEVA LRTRWTPG*FPSSGYCAGLAGHTPGRAGGRSEAG APVGGSQQAPVFSRPSTVITRSRGASSGPGTFL ATRALALLGRAQTQNRPGGGAVSPREGALSRRG Q*PGVLRPPPSKPSAGARGKGSKVTNAKAGRRL* ARRSCWEAWM*GGWGFVAGGSVVRLCQERLNTA GGQSLSSGGRSCRFSAGSVEEGRGWLKWLEVTG KCWRNRLLSGIASQRVTHLLLRNGFIANR*RG*F RQGMHLECFPSARGSPC*GAGAGQGAVTGW*SQ VEDRSDRCGCPGVDVWWRWSSELR*AAANAC*A *TWAGSFRWVAGLKSQGQPRHCPGPSLLAPDACA CFQEHQIHAAADSEPHSLAQALSAQASGPPWDP GRPGPASEVLAFVKAGPCRDRWAGPAPGPWASN PAADHRPVRGWTRWGLAGPEG*EGEAAAAKANRS PAPGSCGPGRLPPAGSQRPGSLQG*LPGPVGAD VLWLHSLP*HLPRRAGEAGAGGAAAGSRAWFAS SAACLHHCGPRAGRR*SHGPLRPGLPPKTVGSDR LHQTGCPG*SQLPRVLQCRPQG*GPGLHRGPLH CHLPAQP*RPLHGLLRPEQIG*ADLRQCAAAHG GFPQCPVLSHCSLGPIIKRAAF

Frame 2

QHENLLPPPHPQVIPFTKSGSRLGPREFTSLPN APGVRVMALDPLPAPSSTQPGRKLAFVLLAPGI RSRPRPAPR*SQGSGKVRSPRICGAGAAATAFC PQVLRAVSAPHPRYGLGRLGGPLAATPAPRPHRG ALRRPRAQIQAAAQHR*KGQAPGLRGGTAPGGG KGKRPGRPALGAEPCLLEGRGGLCRGRVRVADG AWGWARPDANSDAPRLRPA*PVAAARPRLHLRRA LPHAADDVRPRARPPGARSHDRARHRRRPRLLH LRPPTPLSALPHSGTWSGPPGPWPPQRRTASRE AHLGTPDLNPESPSDTLTPHIRYPHIRISNPKPR TPRGFDKSWLRLPTSPRTPSRVPTRLPRSSSPP HP*SAPQAGTSRSERGTRRTPVRLGTPLLPETG VSRRNSRAQVPRPIRCPRSWVRASSAKSPPQLAP STAAERLAVSSRTSARS*VQGSTSPGPPSSRFW RGLRSPKTTLGSQGPRPTTWISTGSPAAGVSGS GTRTTWPRW*PTRTTDPPGGSGPTRVCLKSASPH RVRLSLSLSARPGARPGVEPRLGPCPNSEPSGA GPERRARPGPQRCAASERRDPTEGGRGTLGSHR RPRPTPGGTGQGSPPPTAPGPDGPEVPRVRGHRG IGRGAACKASRGRRLPSSALQAAPPACRPGAPP PRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQA GEGSALSRYGRGSSREAGKREADTDEGDTDTGPR GGHRRDTGKGSGQEHVAQTPTPGGRRPRTCQAS PQARSDGSLDDPGNRGPTRAW*LLRGREPGTSR PFARAQAAPGADPHEARRRPPERSGHQGLRGRCG EWERAGRTDRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQGANDQRTWSGAHRRHLG*AGVYS WIQCHPEPRADASAAGPGGLLYRGSE*AAGSCG RNPICSQRCDSHRGQPATHHSLHSQ*ETRGGAV RSGGGR*VRRGRRLPQPGAGPGAGKDAGWRGSQP RASGRGSADRHGQAPGSLRGPRPGGGGGAALHG RRRPARLKGPGHHARGRPALAQRTRGDSGPGRC PGGRGAGRRLGPWPLRADAGGAGRGSRSGPSPVL GKSRRTPAAAASRPGAGGAAGARRWHRGAGPGA AAGAGAARARGRAQPRWGAAPPGGGRRAAGRRG SEAAPWDPLAPRAPGRPRAQRPAEPRPAGGARTL RPRAIRRPLALRRARSAAAAIKLLCRETLSVLG RERKDPGLRRRGPSR*TRDPRRAPPSAHALRAC QRLRPCAHAQLRGRLRPACERVAPAFPEPAGQSA GSWRSALPLVLGPLTRGPAARCGRRGWEGRR*P *GLAGLRGSFPAPATARGWRGTPQGALEAGARL GRPWEAPSRHRCSRGQAQL*RARAALRVVLEPFW PRGRWPCWGGHKPRTARAGVQ*VLGRVPSAGGG SDRES*DLHLANLLRGPVGKAQRSPTQRQGVGC EPGGAAGKPGCEEGGVLWRVEVSCVSARRG*TQP AGRV*APGVGRAGFLLGVWRKAAVG*SGWR*QE SVGGIGCSRGLQARELPTSF*EMGLLQIDNVVS SGKACTWNAFRQQEVHLAEVQVQGRVR*QAGDPR *RTGVTGVVAQVWMFGGGGVLSSGEQLQMPVKP ERGLGPSDGWLVSSRRGSPGTVLGLPFWLLTPV LVSRSIRSMLLLTRSPTAWHRLSQLKPRVLPGTL GGQALHLRSWLLSRQGPAETGGQGQPQGPGLRT RLLITGLFGAGLGGAWLALRAEKERLQQQKRTE ALRQAAVGQGDFHLLDHRGRARCKADFRGQWVLM YFGFTHCPDICPDELEKLVQVVRQLEAEPGLPP VQPVFITVDPERDDVEAMARYVQDFHPRLLGLT GSTKQVAQASHSYRVYYNAGPKDEDQDYIVDHSI AIYLLNPDGLFTDYYGRSRSAEQISDSVRRHMA AFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.cAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGG CCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCA GCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATC AGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGC TGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAG CCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCC AACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCC TGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGG GGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTC GAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGG AGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCG CGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGAC GGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCG TTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGA TGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGC GCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGAC GCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACT GCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCC AAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAAC CGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGG GGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGG TTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCT GCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAG TTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTC ACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTT TGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGC AGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCC CCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCA GGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCC TGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGG ATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCT GGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTT GAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGT ACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTA CGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCA TCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK RDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLM AIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQ QLVDKHSTGGVGDKVSLVLAPALAACGCKVPMI SGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVL LDQAGCCIVGQSEQLVPADGILYAARDVTATVDS LPLITASILSKKLVEGLSALVVDVKFGGAAVFP NQEQARELAKTLVGVGASLGLRVAAALTAMDKP LGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLG GALLWLSGHAGTQAQGAARVAAALDDGSALGRF ERMLAAQGVDPGLARALCSGSPAERRQLLPRAR EQEELLAPADGTVELVRALPLALVLHELGAGRSR AGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRD GPALSGPQSRALQEALVLSDRAPFAAPSPFAEL VLPPQQ*SSFAAKPCQCLGGSGRIQGCGGGGRLD EHVTPGGLRLPRTR*EPVSGCARVRMRSSGDAC ALPVSVWRPLSLSRRGRAQGAGGRRFLSCLVH* RAAPPRGADAGAGRGGGSPEDSLDSGVVSQLRLL RGAGGAHPRARWRPERGWGARGRLPAGTGVLAA KHSYNALARRFEWSWNLSGHEGAGLAGEGTNPE PPGRGCSESSGGCPQQEGAVTGSPETST*QTFCG GPWERLKGHQRKGRASAVSPEELLGSLDVRRVG FCGGWKCRASLPGEVKHSRRAESELRG*VVQVF CWECGGRPRLVEVAGGNRKVLEESVALGDCKPES YPPPFKKWVYCK*ITWLVQARHALGMLSVSKRF TLLRCRCRAGCGDRLVIPGRGQE*QVWLPRCGC LVEVEF*AQVSSCKCLLSLNVGWVLQMGGWSQVA GAAQALSWAFPSGS*RLCLFPGASDPCCC*LGA PQLGTGSLSSSLGSSLGPWEARPCI*GPGFCQG RALQRQVGRASPRALGFEPGC*SQACSGLDSVGP GWP*GLRRRGCSSKSEQKPCARQLWARATSTCW ITEAGLAARLTSGASGC*CTLASLTALTSAQTS WRSWCRWCGSWKQSLVCLQCSLSSSLWTPSGTTL KPWPATSRTSTQDCWV*PAPPNRLPRLVTVTAC TTMQAPRMRTRTTSWTTPLPSTCSTLTASSRIT TAGADRLSRSQTVCGGTWRLSAVSCLEPLQSGPH H*TGCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGIPAGPERWQP**PREP GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S ETEAA*AKRTSGASWPLW*MGARRAHRSGPC*W PSDFGAWIWRRPRC*PRPWLSRDSSWSGQRPGA SSLWTSIPQGVWVTRSAWSSHLPWRHVAARCQ*S ADVVWGTQEAPWISWSLFLDSMSSRAQSRCKCC WTRRAAVSWVRVSSWFLRTESYMQPEM*QPPWT ACHSSQPPFSVRNSWRGCPLWWWTLSSEGPPSSP TRSRPGSWQRRWLAWEPA*GFGSRQR*PPWTSP WVAAWATPWRWRRRCSAWTAQARQT*GTWSPRS GAPCSGSADTRGLRPRALPGWPRRWTTARPLAAS SGCWRRRAWIPVWPEPCAREVPQNAGSCCLAPG SRRSCWRPQMAPWSWSGRCRWRWCCTSSGPGAA ALGSRSAWGWAQSCWSTWVRGCAVGPPGSACTGT APRSAARRAAPCRRRSYSPTARHSPPPRPSQSS FCRRSNKAPLPRNLVSAWAGAEGSRAAEAGAVS MNT*PPAGSAFRARAESLSAAAPVCACAAPGTPA PCL*ACGARFP*AGGAERRELEVGASSRAWSTD ARPRREVRTPGLGGEEVALRTRWTPG*FPSSGY CAGLAGHTPGRAGGRSEAGAPVGGSQQAPVFSRP STVITRSRGASSGPGTFLATRALALLGRAQTQN RPGGGAVSPREGALSRRGQ*PGVLRPPPSKPSA GARGKGSKVTNAKAGRRL*ARRSCWEAWM*GGWG FVAGGSVVRLCQERLNTAGGQSLSSGGRSCRFS AGSVEEGRGWLKWLEVTGKCWRNRLLSGIASQR VTHLLLRNGFIANR*RG*FRQGMHLECFPSARGS PC*GAGAGQGAVTGW*SQVEDRSDRCGCPGVDV WWRWSSELR*AAANAC*A*TWAGSFRWVAGLKS QGQPRHCPGPSLLAPDACACFQEHQIHAAADSEP HSLAQALSAQASGPPWDPGRPGPASEVLAFVKA GPCRDRWAGPAPGPWASNPAADHRPVRGWTRWG LAGPEG*EGEAAAAKANRSPAPGSCGPGRLPPAG SQRPGSLQG*LPGPVGADVLWLHSLP*HLPRRA GEAGAGGAAAGSRAWFASSAACLHHCGPRAGRR *SHGPLRPGLPPKTVGSDRLHQTGCPG*SQLPRV LQCRPQG*GPGLHRGPLHCHLPAQP*RPLHGLL RPEQIG*ADLRQCAAAHGGFPQCPVLSHCSLGP IIKRAAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE ARRRPPERSGHQGLRGRCGEWERAGRTDRGHADG HPTSGHGSGGDLGADPGPGSVGTAAGVARGLAP AACGQAFHRGCG*QGQPGPRTCPGGMWLQGAND QRTWSGAHRRHLG*AGVYSWIQCHPEPRADASAA GPGGLLYRGSE*AAGSCGRNPICSQRCDSHRGQ PATHHSLHSQ*ETRGGAVRSGGGR*VRRGRRLP QPGAGPGAGKDAGWRGSQPRASGRGSADRHGQAP GSLRGPRPGGGGGAALHGRRRPARLKGPGHHAR GRPALAQRTRGDSGPGRCPGGRGAGRRLGPWPL RADAGGAGRGSRSGPSPVLGKSRRTPAAAASRPG AGGAAGARRWHRGAGPGAAAGAGAARARGRAQP RWGAAPPGGGRRAAGRRGSEAAPWDPLAPRAPG RPRAQRPAEPRPAGGARTLRPRAIRRPLALRRAR SAAAAIKLLCRETLSVLGRERKDPGLRRRGPSR *TRDPRRAPPSAHALRACQRLRPCAHAQLRGRL RPACERVAPAFPEPAGQSAGSWRSALPLVLGPLT RGPAARCGRRGWEGRR*P*GLAGLRGSFPAPAT ARGWRGTPQGALEAGARLGRPWEAPSRHRCSRG QAQL*RARAALRVVLEPFWPRGRWPCWGGHKPRT ARAGVQ*VLGRVPSAGGGSDRES*DLHLANLLR GPVGKAQRSPTQRQGVGCEPGGAAGKPGCEEGG VLWRVEVSCVSARRG*TQPAGRV*APGVGRAGFL LGVWRKAAVG*SGWR*QESVGGIGCSRGLQARE LPTSF*EMGLLQIDNVVSSGKACTWNAFRQQEV HLAEVQVQGRVR*QAGDPR*RTGVTGVVAQVWMF GGGGVLSSGEQLQMPVKPERGLGPSDGWLVSSR RGSPGTVLGLPFWLLTPVLVSRSIRSMLLLTRS PTAWHRLSQLKPRVLPGTLGGQALHLRSWLLSRQ GPAETGGQGQPQGPGLRTRLLITGLFGAGLGGA WLALRAEKERLQQQKRTEALRQAAVGQGDFHLL DHRGRARCKADFRGQWVLMYFGFTHCPDICPDEL EKLVQVVRQLEAEPGLPPVQPVFITVDPERDDV EAMARYVQDFHPRLLGLTGSTKQVAQASHSYRV YYNAGPKDEDQDYIVDHSIAIYLLNPDGLFTDYY GRSRSAEQISDSVRRHMAAFRSVLS*ATAVWAP SLNGLRL

Sequence ECGF1.fAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCC TGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGG CATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAG CATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGG GGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTG CTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACA GCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACG TTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGC GGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCA GACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGC TGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGA ACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTG CTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTG GGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATT CGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATC CAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTG TGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCG GCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGAC TCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGC TCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGG CCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCT GAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGC TGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGA GCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCG GTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCAC TTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTG ACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTC CTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCAT CAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTG CATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAG GCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGC TGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACT CACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCA TCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGT TGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTC AACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCC TGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRAQAAPGADPHEARRRPP ERSGHQGLRGRCGEWERAGRTDRGHADGHPTSG HGSGGDLGADPGPGSVGTAAGVARGLAPAACGQ AFHRGCG*QGQPGPRTCPGGMWLQGANDQRTWSG AHRRHLG*AGVYSWIQCHPEPRADASAAGPGGL LYRGSE*AAGSCGRNPICSQRCDSHRGQPATHH SSRFSIVSNLLCLPQSLHSQ*ETRGGAVRSGGGR *VRRGRRLPQPGAGPGAGKDAGWRGSQPRASGR GSADRHGQAPGSLRGPRPGGGGGAALHGRRRPA RLKGPGHHARGRPALAQRTRGDSGPGRCPGGRGA GRRLGPWPLRADAGGAGRGSRSGPSPVLGKSRR TPAAAASRPGAGGAAGARRWHRGAGPGAAAGAG AARARGRAQPRWGAAPPGGGRRAAGRRGSEAAPW DPLAPRAPGRPRAQRPAEPRPAGGARTLRPRAI RRPLALRRARSAAAAIKLLCRETLSVLGRERKD PGLRRRGPSR*TRDPRRAPPSAHALRACQRLRPC AHAQLRGRLRPACERVAPAFPEPAGQSAGSWRS ALPLVLGPLTRGPAARCGRRGWEGRR*P*GLAG LRGSFPAPATARGWRGTPQGALEAGARLGRPWEA PSRHRCSRGQAQL*RARAALRVVLEPFWPRGRW PCWGGHKPRTARAGVQ*VLGRVPSAGGGSDRES *DLHLANLLRGPVGKAQRSPTQRQGVGCEPGGAA GKPGCEEGGVLWRVEVSCVSARRG*TQPAGRV* APGVGRAGFLLGVWRKAAVG*SGWR*QESVGGI GCSRGLQARELPTSF*EMGLLQIDNVVSSGKACT WNAFRQQEVHLAEVQVQGRVR*QAGDPR*RTGV TGVVAQVWMFGGGGVLSSGEQLQMPVKPERGLG PSDGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSI RSMLLLTRSPTAWHRLSQLKPRVLPGTLGGQAL HLRSWLLSRQGPAETGGQGQPQGPGLRTRLLIT GLFGAGLGGAWLALRAEKERLQQQKRTEALRQAA VGQGDFHLLDHRGRARCKADFRGQWVLMYFGFT HCPDICPDELEKLVQVVRQLEAEPGLPPVQPVF ITVDPERDDVEAMARYVQDFHPRLLGLTGSTKQV AQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLL NPDGLFTDYYGRSRSAEQISDSVRRHMAAFRSV LS*ATAVWAPSLNGLRL

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AEPKQLPELIRMKRDGGR LSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRG MDLEETSVLTQALAQSGQQLEWPEAWRQQLVDK HSTGGVGDKVSLVLAPALAACGCKVPMISGRGL GHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGC CIVGQSEQLVPADGILYAARDVTATVDSLPLIT APGSPLSPTSSASPKASILSKKLVEGLSALVVD VKFGGAAVFPNQEQARELAKTLVGVGASLGLRVA AALTAMDKPLGRCVGHALEVEEALLCMDGAGPP DLRDLVTTLGGALLWLSGHAGTQAQGAARVAAA LDDGSALGRFERMLAAQGVDPGLARALCSGSPAE RRQLLPRAREQEELLAPADGTVELVRALPLALV LHELGAGRSRAGEPLRLGVGAELLVDVGQRLRR GTPWLRVHRDGPALSGPQSRALQEALVLSDRAPF AAPSPFAELVLPPQQ*SSFAAKPCQCLGGSGRI QGCGGGGRLDEHVTPGGLRLPRTR*EPVSGCAR VRMRSSGDACALPVSVWRPLSLSRRGRAQGAGGR RFLSCLVH*RAAPPRGADAGAGRGGGSPEDSLD SGVVSQLRLLRGAGGAHPRARWRPERGWGARGR LPAGTGVLAAKHSYNALARRFEWSWNLSGHEGAG LAGEGTNPEPPGRGCSESSGGCPQQEGAVTGSP ETST*QTFCGGPWERLKGHQRKGRASAVSPEEL LGSLDVRRVGFCGGWKCRASLPGEVKHSRRAESE LRG*VVQVFCWECGGRPRLVEVAGGNRKVLEES VALGDCKPESYPPPFKKWVYCK*ITWLVQARHA LGMLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE* QVWLPRCGCLVEVEF*AQVSSCKCLLSLNVGWV LQMGGWSQVAGAAQALSWAFPSGS*RLCLFPGA SDPCCC*LGAPQLGTGSLSSSLGSSLGPWEARPC I*GPGFCQGRALQRQVGRASPRALGFEPGC*SQ ACSGLDSVGPGWP*GLRRRGCSSKSEQKPCARQ LWARATSTCWITEAGLAARLTSGASGC*CTLASL TALTSAQTSWRSWCRWCGSWKQSLVCLQCSLSS SLWTPSGTTLKPWPATSRTSTQDCWV*PAPPNR LPRLVTVTACTTMQAPRMRTRTTSWTTPLPSTCS TLTASSRITTAGADRLSRSQTVCGGTWRLSAVS CLEPLQSGPHH*TGCV*

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQSPSSSRS*SA*SETEAA *AKRTSGASWPLW*MGARRAHRSGPC*WPSDFG AWIWRRPRC*PRPWLSRDSSWSGQRPGASSLWTS IPQGVWVTRSAWSSHLPWRHVAARCQ*SADVVW GTQEAPWISWSLFLDSMSSRAQSRCKCCWTRRA AVSWVRVSSWFLRTESYMQPEM*QPPWTACHSSQ LQVLHCLQPPLPPPKPPFSVRNSWRGCPLWWWT LSSEGPPSSPTRSRPGSWQRRWLAWEPA*GFGS RQR*PPWTSPWVAAWATPWRWRRRCSAWTAQARQ T*GTWSPRSGAPCSGSADTRGLRPRALPGWPRR WTTARPLAASSGCWRRRAWIPVWPEPCAREVPQ NAGSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWC CTSSGPGAAALGSRSAWGWAQSCWSTWVRGCAV GPPGSACTGTAPRSAARRAAPCRRRSYSPTARH SPPPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGS RAAEAGAVSMNT*PPAGSAFRARAESLSAAAPV CACAAPGTPAPCL*ACGARFP*AGGAERRELEV GASSRAWSTDARPRREVRTPGLGGEEVALRTRWT PG*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGG SQQAPVFSRPSTVITRSRGASSGPGTFLATRAL ALLGRAQTQNRPGGGAVSPREGALSRRGQ*PGVL RPPPSKPSAGARGKGSKVTNAKAGRRL*ARRSC WEAWM*GGWGFVAGGSVVRLCQERLNTAGGQSL SSGGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNR LLSGIASQRVTHLLLRNGFIANR*RG*FRQGMH LECFPSARGSPC*GAGAGQGAVTGW*SQVEDRS DRCGCPGVDVWWRWSSELR*AAANAC*A*TWAGS FRWVAGLKSQGQPRHCPGPSLLAPDACACFQEH QIHAAADSEPHSLAQALSAQASGPPWDPGRPGP ASEVLAFVKAGPCRDRWAGPAPGPWASNPAADHR PVRGWTRWGLAGPEG*EGEAAAAKANRSPAPGS CGPGRLPPAGSQRPGSLQG*LPGPVGADVLWLH SLP*HLPRRAGEAGAGGAAAGSRAWFASSAACLH HCGPRAGRR*SHGPLRPGLPPKTVGSDRLHQTG CPG*SQLPRVLQCRPQG*GPGLHRGPLHCHLPA QP*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCP VLSHCSLGPIIKRAAF

Sequence ECGF1.kAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGTGCGTGGGGAGGGT TGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGCCCCACTACCAGCACCGGCCCCAGGGTGCCCCA CCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCT GACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAG GTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAGACGGGAGCCTATACCGCACATGCAGCAACCAG TCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAAGACCCTACCAAGGCTTCTCCCCACCCTGCTCCCCAGCACAGTTCTCCCCACC CCGTTCCCCAGCACAGCGCTTGGGGCCCCTCTGGCTCCAGACCAGGCCCCTTGGAGCAGGAAAAAGATCCACTGATGGAATTCAGACCCCTTTCCCCTTG GGTCCCCAGACAGCTCCCCCAAGGGAGGAGCTGAGGACTTTCCTCCCTCTGCCCCAAGCCTTGTTTCCCCAAGGACAGGTACCAACCTCCTCCCCTACTG ACACTTCTCAACCAAGAAAACTTCCTTTCCATTCCCTCACCAGCTGGGCACCCCTATAGCTGCTTAAATACTTTCCAAATCCAGCTGCACTCCTAGCCAG GGAAGGTGAAGGGATGCACAGAGGTGGGGGAGGGGTACTGTGCAGGGTACTCAGCATCCCTGACCACCAGGTGCCAATGATCAGCGGACGTGGTCTGGGG CACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGGTACGGGGCGCCACGGATCAGTCATTAATCC AGGTTGATGATGGAGACCCTGGCCAGAATCACTAAAAGATCACTGGTGGATCATTAGGGTCACTAATGAGAACACTGGTCAAGGTTACTCATGAGTCACT GGGCCTGGGCCGAAATCATCAGTGGAACTTTGATTAGGATCATAAAATGGGAAGTTGGTCAAAATCACAGATGGCTGGCGGGGCACGGTGGCTCACACCT GTAGTCCTAGCACTTGGGGAGGCCGAAGAGGGCAGATCCCTTGAACCCAGGAGTTCAAAACCAGCCTGGATAACACGGCAAAACCCCATCTCTACAAAAT AGTTCGCTGCGTGTGGTGGTGCACGCATGTGGTTCCAGCTACTCAGGAGGCTGAGGCAGGAGGATCACTTGAGCCTGGGAGGTCTAGGCTGCAGTGAGCC GGGACGATGCCACTGCACTCCAGCCTGGGCAACAGAGTGAGACCCTGTCCCAGCACTCTGGGAGGCAGAGGAGCCCAGTTGGAGATCAGCCTGGGTAATA TAGTGAAACTTGATCTCTACAAAAAAAAGAAGAAAAAAAAAAGCCGCGTGTGGTGGTGCGCACCTGTAGTCCCAGCTACTGGGAAGCTGAGGTGGGAGGA TCACTTAAGCCCAGGAGGCAGAGGTCACAATGAGCCGAAATTGTGCCAACTGCACTCCAGCCTGGGCAACAGAGGAAGACTCTTCACAGAAAAAAAAAAA AAAAAAAAAAAAGCTGCTAAGTCATTTACCATAAGTCACTGAGAACAGGGGATGTCTGACCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCG TGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGGTGACCT GACTCCATGGCCTGCTTCTGCATGTTCACAGGCTCCTGACCTCCAAACTCAAGTCAAGGGCCTCTCGTTAGGAGTTACCCGTCACCTGACCGTGTGCCCC CCTACCCCCATCACAAGATGCCTGACCACCACCATGTGGGGTGGCCTGATACTCAACCCACCAGGTGCTGCCACCCCCATAATAAGGGACTTGACCCTCA ATGCTCAGGGCCCCTGACCCCAAAGTCGGCATCCCCGAACTCTCCCAAGAAGCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCT CCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGA GCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK RDGGRLSEADIRGFVAAVVNGSAQGAQIGAWGG LGVPDPDWEVSPRDFGSLGVRRCPTTSTGPRVPH RCGLPPSRVPPHTRGHADGHPTSGHGSGGDLGA DPGPGSVGTAAGVARGLAPAACGQAFHRGCG*Q GQPGPRTCPGGMWLQG*KPPPFQTGAYTAHAATS PSTGSSQPQAWPKASKTLPRLLPTLLPSTVLPT PFPSTALGAPLAPDQAPWSRKKIH*WNSDPFPL GSPDSSPKGGAEDFPPSAPSLVSPRTGTNLLPY* HFSTKKTSFPFPHQLGTPIAA*ILSKSSCTPSQ GR*RDAQRWGRGTVQGTQHP*PPGANDQRTWSG AHRRHLG*AGVYSWIQCHPEPRAGTGRHGSVINP G**WRPWPESLKDHWWIIRVTNENTGQGYS*VT GPGPKSSVEL*LGS*NGKLVKITDGWRGTVAHT CSPSTWGGRRGQIP*TQEFKTSLDNTAKPHLYKI VRCVWWCTHVVPATQEAEAGGSLEPGRSRLQ*A GTMPLHSSLGNRVRPCPSTLGGRGAQLEISLGN IVKLDLYKKKKKKKSRVWWCAPVVPATGKLRWED HLSPGGRGHNEPKLCQLHSSLGNRGRLFTEKKK KKKKAAKSFTISH*EQGMSDQMQVLLDQAGCCI VGQSEQLVPADGILYAARDVTATVDSLPLITGDL TPWPASACSQAPDLQTQVKGLSLGVTRHLTVCP PTPITRCLTTTMWGGLILNPPGAATPIIRDLTL NAQGP*PQSRHPRTLPRSSRFSIVSNLLCLPQSL HSQ*ETRGGAVRSGGGR*VRRGRRLPQPGAGPG AGKDAGWRGSQPRASGRGSADRHG

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGIPAGPERWQP**PREP GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S ETEAA*AKRTSGASWPLW*MGARRAHRSVRGEG WAFLTPTGRSARETLGPWGCDGAPLPAPAPGCP TAVGCHPHAYPHIPGAMLMAIRLRGMDLEETSVL TQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDK VSLVLAPALAACGCKVRNHLLSRREPIPHMQQP VHPQAAPNLKPGPKPPRPYQGFSPPCSPAQFSPP RSPAQRLGPLWLQTRPLGAGKRSTDGIQTPFPL GPQTAPPREELRTFLPLPQALFPQGQVPTSSPT DTSQPRKLPFHSLTSWAPL*LLKYFPNPAALLAR EGEGMHRGGGGVLCRVLSIPDHQVPMISGRGLG HTGGTLDKLESIPGFNVIQSPEQVRGATDQSLI QVDDGDPGQNH*KITGGSLGSLMRTLVKVTHESL GLGRNHQWNFD*DHKMGSWSKSQMAGGARWLTP VVLALGEAEEGRSLEPRSSKPAWITRQNPISTK *FAACGGARMWFQLLRRLRQEDHLSLGGLGCSEP GRCHCTPAWATE*DPVPALWEAEEPSWRSAWVI **NLISTKKRRKKKAACGGAHL*SQLLGS*GGR IT*AQEAEVTMSRNCANCTPAWATEEDSSQKKKK KKKKLLSHLP*VTENRGCLTRCKCCWTRRAAVS WVRVSSWFLRTESYMQPEM*QPPWTACHSSQVT *LHGLLLHVHRLLTSKLKSRASR*ELPVT*PCAP LPPSQDA*PPPCGVA*YSTHQVLPPP**GT*PS MLRAPDPKVGIPELSQEAPGSPLSPTSSASPKA SILSKKLVEGLSALVVDVKFGGAAVFPNQEQARE LAKTLVGVGASLGLRVAAALTAM

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE ARRRPPERSGHQGLRGRCGEWERAGRTDRCVGRV GRS*PRLGGQPERLWVPGGATVPHYQHRPQGAP PLWAATLTRTPTYQGPC*WPSDFGAWIWRRPRC *PRPWLSRDSSWSGQRPGASSLWTSIPQGVWVTR SAWSSHLPWRHVAARLETTSFPDGSLYRTCSNQ SIHRQLPTSSLAQSLQDPTKASPHPAPQHSSPH PVPQHSAWGPSGSRPGPLEQEKDPLMEFRPLSPW VPRQLPQGRS*GLSSLCPKPCFPKDRYQPPPLL TLLNQENFLSIPSPAGHPYSCLNTFQIQLHS*P GKVKGCTEVGEGYCAGYSASLTTRCQ*SADVVWG TQEAPWISWSLFLDSMSSRAQSRYGAPRISH*S RLMMETLARITKRSLVDH*GH**EHWSRLLMSH WAWAEIISGTLIRIIKWEVGQNHRWLAGHGGSHL *S*HLGRPKRADPLNPGVQNQPG*HGKTPSLQN SSLRVVVHACGSSYSGG*GRRIT*AWEV*AAVS RDDATALQPGQQSETLSQHSGRQRSPVGDQPG*Y SET*SLQKKEEKKKPRVVVRTCSPSYWEAEVGG SLKPRRQRSQ*AEIVPTALQPGQQRKTLHRKKK KKKKSC*VIYHKSLRTGDV*PDASAAGPGGLLYR GSE*AAGSCGRNPICSQRCDSHRGQPATHHR*P DSMACFCMFTGS*PPNSSQGPLVRSYPSPDRVP PYPHHKMPDHHHVGWPDTQPTRCCHPHNKGLDPQ CSGPLTPKSASPNSPKKLQVLHCLQPPLPPPKP PFSVRNSWRGCPLWWWTLSSEGPPSSPTRSRPG SWQRRWLAWEPA*GFGSRQR*PPW

Sequence ECGF1.sAug05

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAWGGLGVP DPDWEVSPRDFGSLGVRRCPTTSTGPRVPHRCGL PPSRVPPHTRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQG*KPPPFQTGAYTAHAATSPSTG SSQPQAWPKAS

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSVRGEGWAFL TPTGRSARETLGPWGCDGAPLPAPAPGCPTAVG CHPHAYPHIPGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVRNHLLSRREPIPHMQQPVHPQ AAPNLKPGPKPP

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRCVGRVGRS* PRLGGQPERLWVPGGATVPHYQHRPQGAPPLWA ATLTRTPTYQGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARLETTSFPDGSLYRTCSNQSIHR QLPTSSLAQSLQ

Sequence ECGF1.shed1.aAug05

AGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGC CCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGG AGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGA GCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGC CCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGC GCAGGGCGCACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGC CCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTT CGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGG ACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAG ACGGGAGCCTATACCGCACATGCAGCAACCAGTCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

SGPLCANPEPYGPDPRARPGTWAGIRSKRARAA P*AGTGGAQVARRGSGRQTRTRATQTRDRGADTG ETRERGRDRSTWLRHRRREAADPGRVRHPRRPG AMAALMTPGTGAPPAPGDFSGEGSQGLPDPSPE PKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSA QGAQIGAWGGLGVPDPDWEVSPRDFGSLGVRRC PTTSTGPRVPHRCGLPPSRVPPHTRGHADGHPT SGHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKAS

Frame 1

VDRCARTLNPTVPTRGRGRVPGLGSGASGRGQR PKQVRAGLKSRGGEAGGRHGRGRHRHGTEGRTP ERHGKGVGTGARGSDTDAGRPQTPDVSGIPAGPE RWQP**PREPGPHPRLVTSPGKGARDFPTLRQS PSSSRS*SA*SETEAA*AKRTSGASWPLW*MGA RRAHRSVRGEGWAFLTPTGRSARETLGPWGCDGA PLPAPAPGCPTAVGCHPHAYPHIPGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLV DKHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPP

Frame 2

WTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSA LSRYGRGSSREAGKREADTDEGDTDTGPRGGHR RDTGKGSGQEHVAQTPTPGGRRPRTCQASPQAR SDGSLDDPGNRGPTRAW*LLRGREPGTSRPFARA QAAPGADPHEARRRPPERSGHQGLRGRCGEWER AGRTDRCVGRVGRS*PRLGGQPERLWVPGGATV PHYQHRPQGAPPLWAATLTRTPTYQGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFP DGSLYRTCSNQSIHRQLPTSSLAQSLQ