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Sequences

Sequence ECGF1.bAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGAGCGATGGCA GCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGC TCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGC ACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTG GAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCAT GTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAG CCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGA GATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGT TCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGC GCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTA AGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACG ACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCG GCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCAC GAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCC CCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGC CCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGC TGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCA TGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTT CCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGG GTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAG CAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGC TGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACC TCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAA GCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCG GGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCT CTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAA TGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGT GTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAG ATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATC CATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTG AGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGT TCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGG CCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGC CCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTG TGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCA GGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCT GACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTT GAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPQTPDVSGIPAGPERWQ P**PREPGPHPRLVTSPGKGARDFPTLRQSPSS SRS*SA*SETEAA*AKRTSGASWPLW*MGARRA HRSGPC*WPSDFGAWIWRRPRC*PRPWLSRDSSW SGQRPGASSLWTSIPQGVWVTRSAWSSHLPWRH VAARCQ*SADVVWGTQEAPWISWSLFLDSMSSR AQSRCKCCWTRRAAVSWVRVSSWFLRTESYMQPE M*QPPWTACHSSQPPFSVRNSWRGCPLWWWTLS SEGPPSSPTRSRPGSWQRRWLAWEPA*GFGSRQ R*PPWTSPWVAAWATPWRWRRRCSAWTAQARQT* GTWSPRSGAPCSGSADTRGLRPRALPGWPRRWT TARPLAASSGCWRRRAWIPVWPEPCAREVPQNA GSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWCCT SSGPGAAALGSRSAWGWAQSCWSTWVRGCAVGP PGSACTGTAPRSAARRAAPCRRRSYSPTARHSP PPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGSRA AEAGAVSMNT*PPAGSAFRARAESLSAAAPVCA CAAPGTPAPCL*ACGARFP*AGGAERRELEVGA SSRAWSTDARPRREVRTPGLGGEEVALRTRWTPG *FPSSGYCAGLAGHTPGRAGGRSEAGAPVGGSQ QAPVFSRPSTVITRSRGASSGPGTFLATRALAL LGRAQTQNRPGGGAVSPREGALSRRGQ*PGVLRP PPSKPSAGARGKGSKVTNAKAGRRL*ARRSCWE AWM*GGWGFVAGGSVVRLCQERLNTAGGQSLSS GGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNRLL SGIASQRVTHLLLRNGFIANR*RG*FRQGMHLE CFPSARGSPC*GAGAGQGAVTGW*SQVEDRSDR CGCPGVDVWWRWSSELR*AAANAC*A*TWAGSFR WVAGLKSQGQPRHCPGPSLLAPDACACFQEHQI HAAADSEPHSLAQALSAQASGPPWDPGRPGPAS EVLAFVKAGPCRDRWAGPAPGPWASNPAADHRPV RGWTRWGLAGPEG*EGEAAAAKANRSPAPGSCG PGRLPPAGSQRPGSLQG*LPGPVGADVLWLHSL P*HLPRRAGEAGAGGAAAGSRAWFASSAACLHHC GPRAGRR*SHGPLRPGLPPKTVGSDRLHQTGCP G*SQLPRVLQCRPQG*GPGLHRGPLHCHLPAQP *RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCPVL SHCSLGPIIKRAAF

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGRRPRTCQASPQARSDG SLDDPGNRGPTRAW*LLRGREPGTSRPFARAQAA PGADPHEARRRPPERSGHQGLRGRCGEWERAGR TDRGHADGHPTSGHGSGGDLGADPGPGSVGTAA GVARGLAPAACGQAFHRGCG*QGQPGPRTCPGGM WLQGANDQRTWSGAHRRHLG*AGVYSWIQCHPE PRADASAAGPGGLLYRGSE*AAGSCGRNPICSQ RCDSHRGQPATHHSLHSQ*ETRGGAVRSGGGR*V RRGRRLPQPGAGPGAGKDAGWRGSQPRASGRGS ADRHGQAPGSLRGPRPGGGGGAALHGRRRPARL KGPGHHARGRPALAQRTRGDSGPGRCPGGRGAGR RLGPWPLRADAGGAGRGSRSGPSPVLGKSRRTP AAAASRPGAGGAAGARRWHRGAGPGAAAGAGAA RARGRAQPRWGAAPPGGGRRAAGRRGSEAAPWDP LAPRAPGRPRAQRPAEPRPAGGARTLRPRAIRR PLALRRARSAAAAIKLLCRETLSVLGRERKDPG LRRRGPSR*TRDPRRAPPSAHALRACQRLRPCAH AQLRGRLRPACERVAPAFPEPAGQSAGSWRSAL PLVLGPLTRGPAARCGRRGWEGRR*P*GLAGLR GSFPAPATARGWRGTPQGALEAGARLGRPWEAPS RHRCSRGQAQL*RARAALRVVLEPFWPRGRWPC WGGHKPRTARAGVQ*VLGRVPSAGGGSDRES*D LHLANLLRGPVGKAQRSPTQRQGVGCEPGGAAGK PGCEEGGVLWRVEVSCVSARRG*TQPAGRV*AP GVGRAGFLLGVWRKAAVG*SGWR*QESVGGIGC SRGLQARELPTSF*EMGLLQIDNVVSSGKACTWN AFRQQEVHLAEVQVQGRVR*QAGDPR*RTGVTG VVAQVWMFGGGGVLSSGEQLQMPVKPERGLGPS DGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSIRS MLLLTRSPTAWHRLSQLKPRVLPGTLGGQALHL RSWLLSRQGPAETGGQGQPQGPGLRTRLLITGL FGAGLGGAWLALRAEKERLQQQKRTEALRQAAVG QGDFHLLDHRGRARCKADFRGQWVLMYFGFTHC PDICPDELEKLVQVVRQLEAEPGLPPVQPVFIT VDPERDDVEAMARYVQDFHPRLLGLTGSTKQVAQ ASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLNP DGLFTDYYGRSRSAEQISDSVRRHMAAFRSVLS *ATAVWAPSLNGLRL

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQAADPGRVRHPRRPGAMA ALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQ LPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGA QIGAMLMAIRLRGMDLEETSVLTQALAQSGQQL EWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAA CGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQS PEQMQVLLDQAGCCIVGQSEQLVPADGILYAAR DVTATVDSLPLITASILSKKLVEGLSALVVDVK FGGAAVFPNQEQARELAKTLVGVGASLGLRVAAA LTAMDKPLGRCVGHALEVEEALLCMDGAGPPDL RDLVTTLGGALLWLSGHAGTQAQGAARVAAALD DGSALGRFERMLAAQGVDPGLARALCSGSPAERR QLLPRAREQEELLAPADGTVELVRALPLALVLH ELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGT PWLRVHRDGPALSGPQSRALQEALVLSDRAPFAA PSPFAELVLPPQQ*SSFAAKPCQCLGGSGRIQG CGGGGRLDEHVTPGGLRLPRTR*EPVSGCARVR MRSSGDACALPVSVWRPLSLSRRGRAQGAGGRRF LSCLVH*RAAPPRGADAGAGRGGGSPEDSLDSG VVSQLRLLRGAGGAHPRARWRPERGWGARGRLP AGTGVLAAKHSYNALARRFEWSWNLSGHEGAGLA GEGTNPEPPGRGCSESSGGCPQQEGAVTGSPET ST*QTFCGGPWERLKGHQRKGRASAVSPEELLG SLDVRRVGFCGGWKCRASLPGEVKHSRRAESELR G*VVQVFCWECGGRPRLVEVAGGNRKVLEESVA LGDCKPESYPPPFKKWVYCK*ITWLVQARHALG MLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE*QV WLPRCGCLVEVEF*AQVSSCKCLLSLNVGWVLQ MGGWSQVAGAAQALSWAFPSGS*RLCLFPGASD PCCC*LGAPQLGTGSLSSSLGSSLGPWEARPCI* GPGFCQGRALQRQVGRASPRALGFEPGC*SQAC SGLDSVGPGWP*GLRRRGCSSKSEQKPCARQLW ARATSTCWITEAGLAARLTSGASGC*CTLASLTA LTSAQTSWRSWCRWCGSWKQSLVCLQCSLSSSL WTPSGTTLKPWPATSRTSTQDCWV*PAPPNRLP RLVTVTACTTMQAPRMRTRTTSWTTPLPSTCSTL TASSRITTAGADRLSRSQTVCGGTWRLSAVSCL EPLQSGPHH*TGCV*

Sequence ECGF1.aAug05

AACAGCACGAAAATCTCCTGCCCCCACCCCACCCCCAGGTGATTCCCTTCACCAAGTCTGGGTCTAGACTTGGCCCCCGCGAGTTCACGTCCCTGCCTAA TGCGCCTGGAGTCCGGGTGATGGCCCTGGATCCGCTCCCGGCCCCAAGCTCCACACAGCCGGGAAGGAAGCTCGCGTTTGTTTTGCTGGCGCCAGGGATA CGGTCCAGGCCCCGCCCCGCCCCGCGCTGATCCCAGGGTAGTGGGAAAGTGCGCTCCCCAAGAATTTGCGGTGCAGGGGCCGCGGCCACCGCCTTCTGTC CGCAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGGCCCCATCGCGG CGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGTAGAAGGGGCAGGCCCCGGGATTGAGGGGTGGGACGGCGCCTGGGGGAGGG AAGGGGAAGAGACCAGGGAGGCCTGCGCTCGGGGCTGAACCGTGCCTGCTAGAGGGGAGAGGGGGTCTGTGCCGGGGGAGGGTCCGTGTTGCAGATGGGG CTTGGGGCTGGGCCCGGCCAGACGCTAACTCGGATGCTCCCAGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCCTTCACCTTCGGCGCGC GCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCGCCCCCGCCTACTCCAT CTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGTCAGGACCCCCGGGCCCCTGGCCACCCCAACGCCGAACTGCCTCCAGGGAGG CCCACCTGGGAACCCCCGACCTGAACCCCGAGTCCCCCTCGGATACCCTAACACCGCATATTCGGTACCCCCATATCCGGATCTCAAATCCCAAACCCCG AACCCCACGGGGCTTTGATAAATCGTGGCTCAGACTCCCCACTAGTCCCAGGACCCCATCTCGGGTACCCACCAGGCTCCCACGCAGTTCTAGCCCCCCA CACCCTTGATCCGCCCCGCAGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTACCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGG TGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTGGGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCC ATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGG CGCGGACTTCGCTCCCCCAAGACAACACTCGGAAGCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCG GCACTCGGACTACCTGGCCCCGCTGGTGACCGACGCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCA TCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGTCCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCG GGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGCGCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGAC GCCCGAGACCCACGCCGGGCGGGACCGGCCAGGGATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGG GATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGGGGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCG CCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGG GCGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAG GGGCGGACACCGGAGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCC CCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGAC CCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGG TGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCT GGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTC CTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTC CTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGA CGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCC GCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCC TAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGA CGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCC CGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCT CGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCT GCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGT CAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCT CCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGG CGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTC AGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGC AGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCC TGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGG GGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTG GCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGC AGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTG AGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCC GGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAA AGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTT CAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAG GTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCT GAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGC TTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCT GGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACC CGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAG CCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGAT GTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCA GTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCG GCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCAT TGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCG GCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

NSTKISCPHPTPR*FPSPSLGLDLAPASSRPCL MRLESG*WPWIRSRPQAPHSREGSSRLFCWRQGY GPGPAPPRADPRVVGKCAPQEFAVQGPRPPPSV RRCCEP*APPTRAMGSDAWVGLWRPHRPRGPIA AHYGGPGPKYKLPPNTGRRGRPRD*GVGRRLGEG RGRDQGGLRSGLNRAC*RGEGVCAGGGSVLQMG LGAGPGQTLTRMLPGYALHDPSRPRAPAFTFGA RFPTQQTTCGPGPGHLVPARMTVRGTDGAPAYSI YGRPRRSAPFLTPGPGQDPRAPGHPNAELPPGR PTWEPPT*TPSPPRIP*HRIFGTPISGSQIPNP EPHGALINRGSDSPLVPGPHLGYPPGSHAVLAPH TLDPPRRQVLPGASGERDVPQCASAHHCSPKLG CPGGTAEPRSRGLYGALALGSARHRQSLRPNLL HLRPQSGWQFLRGPQQGRESRGLQVPGPPVHDSG ADFAPPRQHSEARARGLQRGSAPEAPRLEFRDP ALGLPGPAGDRRGQLTRQAGAAPHVFA*SLRVR IVSASLSLSLRVLAQGLGWSHGWGRVPTPNPAGR GPSVGRGRDPSAAPRPNVETPPRAGGGLSGATD ARDPRRAGPARDHPRRRPRAPTARKFRVSGGTG GLAGARRARLPGGGDCRAPPSRRPHPPAVLGRRR PAAGSGPLCANPEPYGPDPRARPGTWAGIRSKR ARAAP*AGTGGAQVARRGSGRQTRTRATQTRDR GADTGETRERGRDRSTWLRHRRREAADPGRVRHP RRPGAMAALMTPGTGAPPAPGDFSGEGSQGLPD PSPEPKQLPELIRMKRDGGRLSEADIRGFVAAV VNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVPMISGRGLGHTGGTLDKLESI PGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAD GILYAARDVTATVDSLPLITASILSKKLVEGLS ALVVDVKFGGAAVFPNQEQARELAKTLVGVGAS LGLRVAAALTAMDKPLGRCVGHALEVEEALLCMD GAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAA RVAAALDDGSALGRFERMLAAQGVDPGLARALC SGSPAERRQLLPRAREQEELLAPADGTVELVRAL PLALVLHELGAGRSRAGEPLRLGVGAELLVDVG QRLRRGTPWLRVHRDGPALSGPQSRALQEALVL SDRAPFAAPSPFAELVLPPQQ*SSFAAKPCQCLG GSGRIQGCGGGGRLDEHVTPGGLRLPRTR*EPV SGCARVRMRSSGDACALPVSVWRPLSLSRRGRA QGAGGRRFLSCLVH*RAAPPRGADAGAGRGGGSP EDSLDSGVVSQLRLLRGAGGAHPRARWRPERGW GARGRLPAGTGVLAAKHSYNALARRFEWSWNLS GHEGAGLAGEGTNPEPPGRGCSESSGGCPQQEGA VTGSPETST*QTFCGGPWERLKGHQRKGRASAV SPEELLGSLDVRRVGFCGGWKCRASLPGEVKHS RRAESELRG*VVQVFCWECGGRPRLVEVAGGNRK VLEESVALGDCKPESYPPPFKKWVYCK*ITWLV QARHALGMLSVSKRFTLLRCRCRAGCGDRLVIP GRGQE*QVWLPRCGCLVEVEF*AQVSSCKCLLSL NVGWVLQMGGWSQVAGAAQALSWAFPSGS*RLC LFPGASDPCCC*LGAPQLGTGSLSSSLGSSLGP WEARPCI*GPGFCQGRALQRQVGRASPRALGFEP GC*SQACSGLDSVGPGWP*GLRRRGCSSKSEQK PCARQLWARATSTCWITEAGLAARLTSGASGC* CTLASLTALTSAQTSWRSWCRWCGSWKQSLVCLQ CSLSSSLWTPSGTTLKPWPATSRTSTQDCWV*P APPNRLPRLVTVTACTTMQAPRMRTRTTSWTTP LPSTCSTLTASSRITTAGADRLSRSQTVCGGTWR LSAVSCLEPLQSGPHH*TGCV*

Frame 1

TARKSPAPTPPPGDSLHQVWV*TWPPRVHVPA* CAWSPGDGPGSAPGPKLHTAGKEARVCFAGARD TVQAPPRPALIPG*WESALPKNLRCRGRGHRLLS AGAASRKRPPPALWARTPGWAFGGHTGPAAPSR RTTEAPGPNTSCRPTPVEGAGPGIEGWDGAWGR EGEETREACARG*TVPARGERGSVPGEGPCCRWG LGLGPARR*LGCSQATPCMTRRGRAPPPSPSAR ASPRSRRRAAPGQATWCPLA*PCAAPTAPPPTP STAAHAAQRPSSLRDLVRTPGPLATPTPNCLQGG PPGNPRPEPRVPLGYPNTAYSVPPYPDLKSQTP NPTGL**IVAQTPH*SQDPISGTHQAPTQF*PP TPLIRPAGRYFPERAGNATYPSAPRHTIAPRNWG VQAEQQSPGPAAYTVPSLLGPRVIGKVSAPTCS IYGRRAAGSFFEDLSKVVSPGVYKSRAPQFTIL ARTSLPQDNTRKPGPAAYNVDQHRKPRGWSFGIR HSDYLAPLVTDADN*PARRERPHTCLLKVCESA SCPPLSLSLCASWRKAWGGATAGAVSQLRTQRG GARASGEAGTPALRRVRTSRPHRGREGDSREPQT PETHAGRDRPGITPADGPGPRRPGSSACPGAPG DWPGRGVQGFPGAATAELRPPGGPTRLPSWGAA APPPAVDRCARTLNPTVPTRGRGRVPGLGSGASG RGQRPKQVRAGLKSRGGEAGGRHGRGRHRHGTE GRTPERHGKGVGTGARGSDTDAGRPQTPDVSGI PAGPERWQP**PREPGPHPRLVTSPGKGARDFPT LRQSPSSSRS*SA*SETEAA*AKRTSGASWPLW *MGARRAHRSGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARCQ*SADVVWGTQEAPWISWSLF LDSMSSRAQSRCKCCWTRRAAVSWVRVSSWFLR TESYMQPEM*QPPWTACHSSQPPFSVRNSWRGCP LWWWTLSSEGPPSSPTRSRPGSWQRRWLAWEPA *GFGSRQR*PPWTSPWVAAWATPWRWRRRCSAW TAQARQT*GTWSPRSGAPCSGSADTRGLRPRALP GWPRRWTTARPLAASSGCWRRRAWIPVWPEPCA REVPQNAGSCCLAPGSRRSCWRPQMAPWSWSGR CRWRWCCTSSGPGAAALGSRSAWGWAQSCWSTWV RGCAVGPPGSACTGTAPRSAARRAAPCRRRSYS PTARHSPPPRPSQSSFCRRSNKAPLPRNLVSAW AGAEGSRAAEAGAVSMNT*PPAGSAFRARAESLS AAAPVCACAAPGTPAPCL*ACGARFP*AGGAER RELEVGASSRAWSTDARPRREVRTPGLGGEEVA LRTRWTPG*FPSSGYCAGLAGHTPGRAGGRSEAG APVGGSQQAPVFSRPSTVITRSRGASSGPGTFL ATRALALLGRAQTQNRPGGGAVSPREGALSRRG Q*PGVLRPPPSKPSAGARGKGSKVTNAKAGRRL* ARRSCWEAWM*GGWGFVAGGSVVRLCQERLNTA GGQSLSSGGRSCRFSAGSVEEGRGWLKWLEVTG KCWRNRLLSGIASQRVTHLLLRNGFIANR*RG*F RQGMHLECFPSARGSPC*GAGAGQGAVTGW*SQ VEDRSDRCGCPGVDVWWRWSSELR*AAANAC*A *TWAGSFRWVAGLKSQGQPRHCPGPSLLAPDACA CFQEHQIHAAADSEPHSLAQALSAQASGPPWDP GRPGPASEVLAFVKAGPCRDRWAGPAPGPWASN PAADHRPVRGWTRWGLAGPEG*EGEAAAAKANRS PAPGSCGPGRLPPAGSQRPGSLQG*LPGPVGAD VLWLHSLP*HLPRRAGEAGAGGAAAGSRAWFAS SAACLHHCGPRAGRR*SHGPLRPGLPPKTVGSDR LHQTGCPG*SQLPRVLQCRPQG*GPGLHRGPLH CHLPAQP*RPLHGLLRPEQIG*ADLRQCAAAHG GFPQCPVLSHCSLGPIIKRAAF

Frame 2

QHENLLPPPHPQVIPFTKSGSRLGPREFTSLPN APGVRVMALDPLPAPSSTQPGRKLAFVLLAPGI RSRPRPAPR*SQGSGKVRSPRICGAGAAATAFC PQVLRAVSAPHPRYGLGRLGGPLAATPAPRPHRG ALRRPRAQIQAAAQHR*KGQAPGLRGGTAPGGG KGKRPGRPALGAEPCLLEGRGGLCRGRVRVADG AWGWARPDANSDAPRLRPA*PVAAARPRLHLRRA LPHAADDVRPRARPPGARSHDRARHRRRPRLLH LRPPTPLSALPHSGTWSGPPGPWPPQRRTASRE AHLGTPDLNPESPSDTLTPHIRYPHIRISNPKPR TPRGFDKSWLRLPTSPRTPSRVPTRLPRSSSPP HP*SAPQAGTSRSERGTRRTPVRLGTPLLPETG VSRRNSRAQVPRPIRCPRSWVRASSAKSPPQLAP STAAERLAVSSRTSARS*VQGSTSPGPPSSRFW RGLRSPKTTLGSQGPRPTTWISTGSPAAGVSGS GTRTTWPRW*PTRTTDPPGGSGPTRVCLKSASPH RVRLSLSLSARPGARPGVEPRLGPCPNSEPSGA GPERRARPGPQRCAASERRDPTEGGRGTLGSHR RPRPTPGGTGQGSPPPTAPGPDGPEVPRVRGHRG IGRGAACKASRGRRLPSSALQAAPPACRPGAPP PRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQA GEGSALSRYGRGSSREAGKREADTDEGDTDTGPR GGHRRDTGKGSGQEHVAQTPTPGGRRPRTCQAS PQARSDGSLDDPGNRGPTRAW*LLRGREPGTSR PFARAQAAPGADPHEARRRPPERSGHQGLRGRCG EWERAGRTDRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQGANDQRTWSGAHRRHLG*AGVYS WIQCHPEPRADASAAGPGGLLYRGSE*AAGSCG RNPICSQRCDSHRGQPATHHSLHSQ*ETRGGAV RSGGGR*VRRGRRLPQPGAGPGAGKDAGWRGSQP RASGRGSADRHGQAPGSLRGPRPGGGGGAALHG RRRPARLKGPGHHARGRPALAQRTRGDSGPGRC PGGRGAGRRLGPWPLRADAGGAGRGSRSGPSPVL GKSRRTPAAAASRPGAGGAAGARRWHRGAGPGA AAGAGAARARGRAQPRWGAAPPGGGRRAAGRRG SEAAPWDPLAPRAPGRPRAQRPAEPRPAGGARTL RPRAIRRPLALRRARSAAAAIKLLCRETLSVLG RERKDPGLRRRGPSR*TRDPRRAPPSAHALRAC QRLRPCAHAQLRGRLRPACERVAPAFPEPAGQSA GSWRSALPLVLGPLTRGPAARCGRRGWEGRR*P *GLAGLRGSFPAPATARGWRGTPQGALEAGARL GRPWEAPSRHRCSRGQAQL*RARAALRVVLEPFW PRGRWPCWGGHKPRTARAGVQ*VLGRVPSAGGG SDRES*DLHLANLLRGPVGKAQRSPTQRQGVGC EPGGAAGKPGCEEGGVLWRVEVSCVSARRG*TQP AGRV*APGVGRAGFLLGVWRKAAVG*SGWR*QE SVGGIGCSRGLQARELPTSF*EMGLLQIDNVVS SGKACTWNAFRQQEVHLAEVQVQGRVR*QAGDPR *RTGVTGVVAQVWMFGGGGVLSSGEQLQMPVKP ERGLGPSDGWLVSSRRGSPGTVLGLPFWLLTPV LVSRSIRSMLLLTRSPTAWHRLSQLKPRVLPGTL GGQALHLRSWLLSRQGPAETGGQGQPQGPGLRT RLLITGLFGAGLGGAWLALRAEKERLQQQKRTE ALRQAAVGQGDFHLLDHRGRARCKADFRGQWVLM YFGFTHCPDICPDELEKLVQVVRQLEAEPGLPP VQPVFITVDPERDDVEAMARYVQDFHPRLLGLT GSTKQVAQASHSYRVYYNAGPKDEDQDYIVDHSI AIYLLNPDGLFTDYYGRSRSAEQISDSVRRHMA AFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.shed1.aAug05

AGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGC CCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGG AGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGA GCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGC CCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGC GCAGGGCGCACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGC CCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTT CGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGG ACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAG ACGGGAGCCTATACCGCACATGCAGCAACCAGTCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

SGPLCANPEPYGPDPRARPGTWAGIRSKRARAA P*AGTGGAQVARRGSGRQTRTRATQTRDRGADTG ETRERGRDRSTWLRHRRREAADPGRVRHPRRPG AMAALMTPGTGAPPAPGDFSGEGSQGLPDPSPE PKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSA QGAQIGAWGGLGVPDPDWEVSPRDFGSLGVRRC PTTSTGPRVPHRCGLPPSRVPPHTRGHADGHPT SGHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKAS

Frame 1

VDRCARTLNPTVPTRGRGRVPGLGSGASGRGQR PKQVRAGLKSRGGEAGGRHGRGRHRHGTEGRTP ERHGKGVGTGARGSDTDAGRPQTPDVSGIPAGPE RWQP**PREPGPHPRLVTSPGKGARDFPTLRQS PSSSRS*SA*SETEAA*AKRTSGASWPLW*MGA RRAHRSVRGEGWAFLTPTGRSARETLGPWGCDGA PLPAPAPGCPTAVGCHPHAYPHIPGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLV DKHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPP

Frame 2

WTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSA LSRYGRGSSREAGKREADTDEGDTDTGPRGGHR RDTGKGSGQEHVAQTPTPGGRRPRTCQASPQAR SDGSLDDPGNRGPTRAW*LLRGREPGTSRPFARA QAAPGADPHEARRRPPERSGHQGLRGRCGEWER AGRTDRCVGRVGRS*PRLGGQPERLWVPGGATV PHYQHRPQGAPPLWAATLTRTPTYQGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFP DGSLYRTCSNQSIHRQLPTSSLAQSLQ