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Sequences

Sequence ECGF1.kAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGTGCGTGGGGAGGGT TGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGCCCCACTACCAGCACCGGCCCCAGGGTGCCCCA CCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCT GACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAG GTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAGACGGGAGCCTATACCGCACATGCAGCAACCAG TCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAAGACCCTACCAAGGCTTCTCCCCACCCTGCTCCCCAGCACAGTTCTCCCCACC CCGTTCCCCAGCACAGCGCTTGGGGCCCCTCTGGCTCCAGACCAGGCCCCTTGGAGCAGGAAAAAGATCCACTGATGGAATTCAGACCCCTTTCCCCTTG GGTCCCCAGACAGCTCCCCCAAGGGAGGAGCTGAGGACTTTCCTCCCTCTGCCCCAAGCCTTGTTTCCCCAAGGACAGGTACCAACCTCCTCCCCTACTG ACACTTCTCAACCAAGAAAACTTCCTTTCCATTCCCTCACCAGCTGGGCACCCCTATAGCTGCTTAAATACTTTCCAAATCCAGCTGCACTCCTAGCCAG GGAAGGTGAAGGGATGCACAGAGGTGGGGGAGGGGTACTGTGCAGGGTACTCAGCATCCCTGACCACCAGGTGCCAATGATCAGCGGACGTGGTCTGGGG CACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGGTACGGGGCGCCACGGATCAGTCATTAATCC AGGTTGATGATGGAGACCCTGGCCAGAATCACTAAAAGATCACTGGTGGATCATTAGGGTCACTAATGAGAACACTGGTCAAGGTTACTCATGAGTCACT GGGCCTGGGCCGAAATCATCAGTGGAACTTTGATTAGGATCATAAAATGGGAAGTTGGTCAAAATCACAGATGGCTGGCGGGGCACGGTGGCTCACACCT GTAGTCCTAGCACTTGGGGAGGCCGAAGAGGGCAGATCCCTTGAACCCAGGAGTTCAAAACCAGCCTGGATAACACGGCAAAACCCCATCTCTACAAAAT AGTTCGCTGCGTGTGGTGGTGCACGCATGTGGTTCCAGCTACTCAGGAGGCTGAGGCAGGAGGATCACTTGAGCCTGGGAGGTCTAGGCTGCAGTGAGCC GGGACGATGCCACTGCACTCCAGCCTGGGCAACAGAGTGAGACCCTGTCCCAGCACTCTGGGAGGCAGAGGAGCCCAGTTGGAGATCAGCCTGGGTAATA TAGTGAAACTTGATCTCTACAAAAAAAAGAAGAAAAAAAAAAGCCGCGTGTGGTGGTGCGCACCTGTAGTCCCAGCTACTGGGAAGCTGAGGTGGGAGGA TCACTTAAGCCCAGGAGGCAGAGGTCACAATGAGCCGAAATTGTGCCAACTGCACTCCAGCCTGGGCAACAGAGGAAGACTCTTCACAGAAAAAAAAAAA AAAAAAAAAAAAGCTGCTAAGTCATTTACCATAAGTCACTGAGAACAGGGGATGTCTGACCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCG TGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGGTGACCT GACTCCATGGCCTGCTTCTGCATGTTCACAGGCTCCTGACCTCCAAACTCAAGTCAAGGGCCTCTCGTTAGGAGTTACCCGTCACCTGACCGTGTGCCCC CCTACCCCCATCACAAGATGCCTGACCACCACCATGTGGGGTGGCCTGATACTCAACCCACCAGGTGCTGCCACCCCCATAATAAGGGACTTGACCCTCA ATGCTCAGGGCCCCTGACCCCAAAGTCGGCATCCCCGAACTCTCCCAAGAAGCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCT CCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGA GCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK RDGGRLSEADIRGFVAAVVNGSAQGAQIGAWGG LGVPDPDWEVSPRDFGSLGVRRCPTTSTGPRVPH RCGLPPSRVPPHTRGHADGHPTSGHGSGGDLGA DPGPGSVGTAAGVARGLAPAACGQAFHRGCG*Q GQPGPRTCPGGMWLQG*KPPPFQTGAYTAHAATS PSTGSSQPQAWPKASKTLPRLLPTLLPSTVLPT PFPSTALGAPLAPDQAPWSRKKIH*WNSDPFPL GSPDSSPKGGAEDFPPSAPSLVSPRTGTNLLPY* HFSTKKTSFPFPHQLGTPIAA*ILSKSSCTPSQ GR*RDAQRWGRGTVQGTQHP*PPGANDQRTWSG AHRRHLG*AGVYSWIQCHPEPRAGTGRHGSVINP G**WRPWPESLKDHWWIIRVTNENTGQGYS*VT GPGPKSSVEL*LGS*NGKLVKITDGWRGTVAHT CSPSTWGGRRGQIP*TQEFKTSLDNTAKPHLYKI VRCVWWCTHVVPATQEAEAGGSLEPGRSRLQ*A GTMPLHSSLGNRVRPCPSTLGGRGAQLEISLGN IVKLDLYKKKKKKKSRVWWCAPVVPATGKLRWED HLSPGGRGHNEPKLCQLHSSLGNRGRLFTEKKK KKKKAAKSFTISH*EQGMSDQMQVLLDQAGCCI VGQSEQLVPADGILYAARDVTATVDSLPLITGDL TPWPASACSQAPDLQTQVKGLSLGVTRHLTVCP PTPITRCLTTTMWGGLILNPPGAATPIIRDLTL NAQGP*PQSRHPRTLPRSSRFSIVSNLLCLPQSL HSQ*ETRGGAVRSGGGR*VRRGRRLPQPGAGPG AGKDAGWRGSQPRASGRGSADRHG

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGIPAGPERWQP**PREP GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S ETEAA*AKRTSGASWPLW*MGARRAHRSVRGEG WAFLTPTGRSARETLGPWGCDGAPLPAPAPGCP TAVGCHPHAYPHIPGAMLMAIRLRGMDLEETSVL TQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDK VSLVLAPALAACGCKVRNHLLSRREPIPHMQQP VHPQAAPNLKPGPKPPRPYQGFSPPCSPAQFSPP RSPAQRLGPLWLQTRPLGAGKRSTDGIQTPFPL GPQTAPPREELRTFLPLPQALFPQGQVPTSSPT DTSQPRKLPFHSLTSWAPL*LLKYFPNPAALLAR EGEGMHRGGGGVLCRVLSIPDHQVPMISGRGLG HTGGTLDKLESIPGFNVIQSPEQVRGATDQSLI QVDDGDPGQNH*KITGGSLGSLMRTLVKVTHESL GLGRNHQWNFD*DHKMGSWSKSQMAGGARWLTP VVLALGEAEEGRSLEPRSSKPAWITRQNPISTK *FAACGGARMWFQLLRRLRQEDHLSLGGLGCSEP GRCHCTPAWATE*DPVPALWEAEEPSWRSAWVI **NLISTKKRRKKKAACGGAHL*SQLLGS*GGR IT*AQEAEVTMSRNCANCTPAWATEEDSSQKKKK KKKKLLSHLP*VTENRGCLTRCKCCWTRRAAVS WVRVSSWFLRTESYMQPEM*QPPWTACHSSQVT *LHGLLLHVHRLLTSKLKSRASR*ELPVT*PCAP LPPSQDA*PPPCGVA*YSTHQVLPPP**GT*PS MLRAPDPKVGIPELSQEAPGSPLSPTSSASPKA SILSKKLVEGLSALVVDVKFGGAAVFPNQEQARE LAKTLVGVGASLGLRVAAALTAM

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE ARRRPPERSGHQGLRGRCGEWERAGRTDRCVGRV GRS*PRLGGQPERLWVPGGATVPHYQHRPQGAP PLWAATLTRTPTYQGPC*WPSDFGAWIWRRPRC *PRPWLSRDSSWSGQRPGASSLWTSIPQGVWVTR SAWSSHLPWRHVAARLETTSFPDGSLYRTCSNQ SIHRQLPTSSLAQSLQDPTKASPHPAPQHSSPH PVPQHSAWGPSGSRPGPLEQEKDPLMEFRPLSPW VPRQLPQGRS*GLSSLCPKPCFPKDRYQPPPLL TLLNQENFLSIPSPAGHPYSCLNTFQIQLHS*P GKVKGCTEVGEGYCAGYSASLTTRCQ*SADVVWG TQEAPWISWSLFLDSMSSRAQSRYGAPRISH*S RLMMETLARITKRSLVDH*GH**EHWSRLLMSH WAWAEIISGTLIRIIKWEVGQNHRWLAGHGGSHL *S*HLGRPKRADPLNPGVQNQPG*HGKTPSLQN SSLRVVVHACGSSYSGG*GRRIT*AWEV*AAVS RDDATALQPGQQSETLSQHSGRQRSPVGDQPG*Y SET*SLQKKEEKKKPRVVVRTCSPSYWEAEVGG SLKPRRQRSQ*AEIVPTALQPGQQRKTLHRKKK KKKKSC*VIYHKSLRTGDV*PDASAAGPGGLLYR GSE*AAGSCGRNPICSQRCDSHRGQPATHHR*P DSMACFCMFTGS*PPNSSQGPLVRSYPSPDRVP PYPHHKMPDHHHVGWPDTQPTRCCHPHNKGLDPQ CSGPLTPKSASPNSPKKLQVLHCLQPPLPPPKP PFSVRNSWRGCPLWWWTLSSEGPPSSPTRSRPG SWQRRWLAWEPA*GFGSRQR*PPW

Sequence ECGF1.cAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGG CCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCA GCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATC AGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGC TGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAG CCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCC AACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCC TGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGG GGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTC GAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGG AGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCG CGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGAC GGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCG TTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGA TGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGC GCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGAC GCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACT GCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCC AAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAAC CGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGG GGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGG TTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCT GCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAG TTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTC ACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTT TGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGC AGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCC CCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCA GGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCC TGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGG ATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCT GGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTT GAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGT ACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTA CGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCA TCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK RDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLM AIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQ QLVDKHSTGGVGDKVSLVLAPALAACGCKVPMI SGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVL LDQAGCCIVGQSEQLVPADGILYAARDVTATVDS LPLITASILSKKLVEGLSALVVDVKFGGAAVFP NQEQARELAKTLVGVGASLGLRVAAALTAMDKP LGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLG GALLWLSGHAGTQAQGAARVAAALDDGSALGRF ERMLAAQGVDPGLARALCSGSPAERRQLLPRAR EQEELLAPADGTVELVRALPLALVLHELGAGRSR AGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRD GPALSGPQSRALQEALVLSDRAPFAAPSPFAEL VLPPQQ*SSFAAKPCQCLGGSGRIQGCGGGGRLD EHVTPGGLRLPRTR*EPVSGCARVRMRSSGDAC ALPVSVWRPLSLSRRGRAQGAGGRRFLSCLVH* RAAPPRGADAGAGRGGGSPEDSLDSGVVSQLRLL RGAGGAHPRARWRPERGWGARGRLPAGTGVLAA KHSYNALARRFEWSWNLSGHEGAGLAGEGTNPE PPGRGCSESSGGCPQQEGAVTGSPETST*QTFCG GPWERLKGHQRKGRASAVSPEELLGSLDVRRVG FCGGWKCRASLPGEVKHSRRAESELRG*VVQVF CWECGGRPRLVEVAGGNRKVLEESVALGDCKPES YPPPFKKWVYCK*ITWLVQARHALGMLSVSKRF TLLRCRCRAGCGDRLVIPGRGQE*QVWLPRCGC LVEVEF*AQVSSCKCLLSLNVGWVLQMGGWSQVA GAAQALSWAFPSGS*RLCLFPGASDPCCC*LGA PQLGTGSLSSSLGSSLGPWEARPCI*GPGFCQG RALQRQVGRASPRALGFEPGC*SQACSGLDSVGP GWP*GLRRRGCSSKSEQKPCARQLWARATSTCW ITEAGLAARLTSGASGC*CTLASLTALTSAQTS WRSWCRWCGSWKQSLVCLQCSLSSSLWTPSGTTL KPWPATSRTSTQDCWV*PAPPNRLPRLVTVTAC TTMQAPRMRTRTTSWTTPLPSTCSTLTASSRIT TAGADRLSRSQTVCGGTWRLSAVSCLEPLQSGPH H*TGCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGIPAGPERWQP**PREP GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S ETEAA*AKRTSGASWPLW*MGARRAHRSGPC*W PSDFGAWIWRRPRC*PRPWLSRDSSWSGQRPGA SSLWTSIPQGVWVTRSAWSSHLPWRHVAARCQ*S ADVVWGTQEAPWISWSLFLDSMSSRAQSRCKCC WTRRAAVSWVRVSSWFLRTESYMQPEM*QPPWT ACHSSQPPFSVRNSWRGCPLWWWTLSSEGPPSSP TRSRPGSWQRRWLAWEPA*GFGSRQR*PPWTSP WVAAWATPWRWRRRCSAWTAQARQT*GTWSPRS GAPCSGSADTRGLRPRALPGWPRRWTTARPLAAS SGCWRRRAWIPVWPEPCAREVPQNAGSCCLAPG SRRSCWRPQMAPWSWSGRCRWRWCCTSSGPGAA ALGSRSAWGWAQSCWSTWVRGCAVGPPGSACTGT APRSAARRAAPCRRRSYSPTARHSPPPRPSQSS FCRRSNKAPLPRNLVSAWAGAEGSRAAEAGAVS MNT*PPAGSAFRARAESLSAAAPVCACAAPGTPA PCL*ACGARFP*AGGAERRELEVGASSRAWSTD ARPRREVRTPGLGGEEVALRTRWTPG*FPSSGY CAGLAGHTPGRAGGRSEAGAPVGGSQQAPVFSRP STVITRSRGASSGPGTFLATRALALLGRAQTQN RPGGGAVSPREGALSRRGQ*PGVLRPPPSKPSA GARGKGSKVTNAKAGRRL*ARRSCWEAWM*GGWG FVAGGSVVRLCQERLNTAGGQSLSSGGRSCRFS AGSVEEGRGWLKWLEVTGKCWRNRLLSGIASQR VTHLLLRNGFIANR*RG*FRQGMHLECFPSARGS PC*GAGAGQGAVTGW*SQVEDRSDRCGCPGVDV WWRWSSELR*AAANAC*A*TWAGSFRWVAGLKS QGQPRHCPGPSLLAPDACACFQEHQIHAAADSEP HSLAQALSAQASGPPWDPGRPGPASEVLAFVKA GPCRDRWAGPAPGPWASNPAADHRPVRGWTRWG LAGPEG*EGEAAAAKANRSPAPGSCGPGRLPPAG SQRPGSLQG*LPGPVGADVLWLHSLP*HLPRRA GEAGAGGAAAGSRAWFASSAACLHHCGPRAGRR *SHGPLRPGLPPKTVGSDRLHQTGCPG*SQLPRV LQCRPQG*GPGLHRGPLHCHLPAQP*RPLHGLL RPEQIG*ADLRQCAAAHGGFPQCPVLSHCSLGP IIKRAAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE ARRRPPERSGHQGLRGRCGEWERAGRTDRGHADG HPTSGHGSGGDLGADPGPGSVGTAAGVARGLAP AACGQAFHRGCG*QGQPGPRTCPGGMWLQGAND QRTWSGAHRRHLG*AGVYSWIQCHPEPRADASAA GPGGLLYRGSE*AAGSCGRNPICSQRCDSHRGQ PATHHSLHSQ*ETRGGAVRSGGGR*VRRGRRLP QPGAGPGAGKDAGWRGSQPRASGRGSADRHGQAP GSLRGPRPGGGGGAALHGRRRPARLKGPGHHAR GRPALAQRTRGDSGPGRCPGGRGAGRRLGPWPL RADAGGAGRGSRSGPSPVLGKSRRTPAAAASRPG AGGAAGARRWHRGAGPGAAAGAGAARARGRAQP RWGAAPPGGGRRAAGRRGSEAAPWDPLAPRAPG RPRAQRPAEPRPAGGARTLRPRAIRRPLALRRAR SAAAAIKLLCRETLSVLGRERKDPGLRRRGPSR *TRDPRRAPPSAHALRACQRLRPCAHAQLRGRL RPACERVAPAFPEPAGQSAGSWRSALPLVLGPLT RGPAARCGRRGWEGRR*P*GLAGLRGSFPAPAT ARGWRGTPQGALEAGARLGRPWEAPSRHRCSRG QAQL*RARAALRVVLEPFWPRGRWPCWGGHKPRT ARAGVQ*VLGRVPSAGGGSDRES*DLHLANLLR GPVGKAQRSPTQRQGVGCEPGGAAGKPGCEEGG VLWRVEVSCVSARRG*TQPAGRV*APGVGRAGFL LGVWRKAAVG*SGWR*QESVGGIGCSRGLQARE LPTSF*EMGLLQIDNVVSSGKACTWNAFRQQEV HLAEVQVQGRVR*QAGDPR*RTGVTGVVAQVWMF GGGGVLSSGEQLQMPVKPERGLGPSDGWLVSSR RGSPGTVLGLPFWLLTPVLVSRSIRSMLLLTRS PTAWHRLSQLKPRVLPGTLGGQALHLRSWLLSRQ GPAETGGQGQPQGPGLRTRLLITGLFGAGLGGA WLALRAEKERLQQQKRTEALRQAAVGQGDFHLL DHRGRARCKADFRGQWVLMYFGFTHCPDICPDEL EKLVQVVRQLEAEPGLPPVQPVFITVDPERDDV EAMARYVQDFHPRLLGLTGSTKQVAQASHSYRV YYNAGPKDEDQDYIVDHSIAIYLLNPDGLFTDYY GRSRSAEQISDSVRRHMAAFRSVLS*ATAVWAP SLNGLRL

Sequence ECGF1.aAug05

AACAGCACGAAAATCTCCTGCCCCCACCCCACCCCCAGGTGATTCCCTTCACCAAGTCTGGGTCTAGACTTGGCCCCCGCGAGTTCACGTCCCTGCCTAA TGCGCCTGGAGTCCGGGTGATGGCCCTGGATCCGCTCCCGGCCCCAAGCTCCACACAGCCGGGAAGGAAGCTCGCGTTTGTTTTGCTGGCGCCAGGGATA CGGTCCAGGCCCCGCCCCGCCCCGCGCTGATCCCAGGGTAGTGGGAAAGTGCGCTCCCCAAGAATTTGCGGTGCAGGGGCCGCGGCCACCGCCTTCTGTC CGCAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGGCCCCATCGCGG CGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGTAGAAGGGGCAGGCCCCGGGATTGAGGGGTGGGACGGCGCCTGGGGGAGGG AAGGGGAAGAGACCAGGGAGGCCTGCGCTCGGGGCTGAACCGTGCCTGCTAGAGGGGAGAGGGGGTCTGTGCCGGGGGAGGGTCCGTGTTGCAGATGGGG CTTGGGGCTGGGCCCGGCCAGACGCTAACTCGGATGCTCCCAGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCCTTCACCTTCGGCGCGC GCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCGCCCCCGCCTACTCCAT CTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGTCAGGACCCCCGGGCCCCTGGCCACCCCAACGCCGAACTGCCTCCAGGGAGG CCCACCTGGGAACCCCCGACCTGAACCCCGAGTCCCCCTCGGATACCCTAACACCGCATATTCGGTACCCCCATATCCGGATCTCAAATCCCAAACCCCG AACCCCACGGGGCTTTGATAAATCGTGGCTCAGACTCCCCACTAGTCCCAGGACCCCATCTCGGGTACCCACCAGGCTCCCACGCAGTTCTAGCCCCCCA CACCCTTGATCCGCCCCGCAGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTACCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGG TGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTGGGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCC ATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGG CGCGGACTTCGCTCCCCCAAGACAACACTCGGAAGCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCG GCACTCGGACTACCTGGCCCCGCTGGTGACCGACGCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCA TCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGTCCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCG GGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGCGCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGAC GCCCGAGACCCACGCCGGGCGGGACCGGCCAGGGATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGG GATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGGGGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCG CCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGG GCGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAG GGGCGGACACCGGAGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCC CCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGAC CCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGG TGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCT GGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTC CTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTC CTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGA CGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCC GCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCC TAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGA CGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCC CGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCT CGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCT GCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGT CAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCT CCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGG CGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTC AGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGC AGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCC TGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGG GGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTG GCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGC AGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTG AGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCC GGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAA AGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTT CAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAG GTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCT GAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGC TTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCT GGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACC CGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAG CCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGAT GTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCA GTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCG GCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCAT TGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCG GCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

NSTKISCPHPTPR*FPSPSLGLDLAPASSRPCL MRLESG*WPWIRSRPQAPHSREGSSRLFCWRQGY GPGPAPPRADPRVVGKCAPQEFAVQGPRPPPSV RRCCEP*APPTRAMGSDAWVGLWRPHRPRGPIA AHYGGPGPKYKLPPNTGRRGRPRD*GVGRRLGEG RGRDQGGLRSGLNRAC*RGEGVCAGGGSVLQMG LGAGPGQTLTRMLPGYALHDPSRPRAPAFTFGA RFPTQQTTCGPGPGHLVPARMTVRGTDGAPAYSI YGRPRRSAPFLTPGPGQDPRAPGHPNAELPPGR PTWEPPT*TPSPPRIP*HRIFGTPISGSQIPNP EPHGALINRGSDSPLVPGPHLGYPPGSHAVLAPH TLDPPRRQVLPGASGERDVPQCASAHHCSPKLG CPGGTAEPRSRGLYGALALGSARHRQSLRPNLL HLRPQSGWQFLRGPQQGRESRGLQVPGPPVHDSG ADFAPPRQHSEARARGLQRGSAPEAPRLEFRDP ALGLPGPAGDRRGQLTRQAGAAPHVFA*SLRVR IVSASLSLSLRVLAQGLGWSHGWGRVPTPNPAGR GPSVGRGRDPSAAPRPNVETPPRAGGGLSGATD ARDPRRAGPARDHPRRRPRAPTARKFRVSGGTG GLAGARRARLPGGGDCRAPPSRRPHPPAVLGRRR PAAGSGPLCANPEPYGPDPRARPGTWAGIRSKR ARAAP*AGTGGAQVARRGSGRQTRTRATQTRDR GADTGETRERGRDRSTWLRHRRREAADPGRVRHP RRPGAMAALMTPGTGAPPAPGDFSGEGSQGLPD PSPEPKQLPELIRMKRDGGRLSEADIRGFVAAV VNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVPMISGRGLGHTGGTLDKLESI PGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAD GILYAARDVTATVDSLPLITASILSKKLVEGLS ALVVDVKFGGAAVFPNQEQARELAKTLVGVGAS LGLRVAAALTAMDKPLGRCVGHALEVEEALLCMD GAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAA RVAAALDDGSALGRFERMLAAQGVDPGLARALC SGSPAERRQLLPRAREQEELLAPADGTVELVRAL PLALVLHELGAGRSRAGEPLRLGVGAELLVDVG QRLRRGTPWLRVHRDGPALSGPQSRALQEALVL SDRAPFAAPSPFAELVLPPQQ*SSFAAKPCQCLG GSGRIQGCGGGGRLDEHVTPGGLRLPRTR*EPV SGCARVRMRSSGDACALPVSVWRPLSLSRRGRA QGAGGRRFLSCLVH*RAAPPRGADAGAGRGGGSP EDSLDSGVVSQLRLLRGAGGAHPRARWRPERGW GARGRLPAGTGVLAAKHSYNALARRFEWSWNLS GHEGAGLAGEGTNPEPPGRGCSESSGGCPQQEGA VTGSPETST*QTFCGGPWERLKGHQRKGRASAV SPEELLGSLDVRRVGFCGGWKCRASLPGEVKHS RRAESELRG*VVQVFCWECGGRPRLVEVAGGNRK VLEESVALGDCKPESYPPPFKKWVYCK*ITWLV QARHALGMLSVSKRFTLLRCRCRAGCGDRLVIP GRGQE*QVWLPRCGCLVEVEF*AQVSSCKCLLSL NVGWVLQMGGWSQVAGAAQALSWAFPSGS*RLC LFPGASDPCCC*LGAPQLGTGSLSSSLGSSLGP WEARPCI*GPGFCQGRALQRQVGRASPRALGFEP GC*SQACSGLDSVGPGWP*GLRRRGCSSKSEQK PCARQLWARATSTCWITEAGLAARLTSGASGC* CTLASLTALTSAQTSWRSWCRWCGSWKQSLVCLQ CSLSSSLWTPSGTTLKPWPATSRTSTQDCWV*P APPNRLPRLVTVTACTTMQAPRMRTRTTSWTTP LPSTCSTLTASSRITTAGADRLSRSQTVCGGTWR LSAVSCLEPLQSGPHH*TGCV*

Frame 1

TARKSPAPTPPPGDSLHQVWV*TWPPRVHVPA* CAWSPGDGPGSAPGPKLHTAGKEARVCFAGARD TVQAPPRPALIPG*WESALPKNLRCRGRGHRLLS AGAASRKRPPPALWARTPGWAFGGHTGPAAPSR RTTEAPGPNTSCRPTPVEGAGPGIEGWDGAWGR EGEETREACARG*TVPARGERGSVPGEGPCCRWG LGLGPARR*LGCSQATPCMTRRGRAPPPSPSAR ASPRSRRRAAPGQATWCPLA*PCAAPTAPPPTP STAAHAAQRPSSLRDLVRTPGPLATPTPNCLQGG PPGNPRPEPRVPLGYPNTAYSVPPYPDLKSQTP NPTGL**IVAQTPH*SQDPISGTHQAPTQF*PP TPLIRPAGRYFPERAGNATYPSAPRHTIAPRNWG VQAEQQSPGPAAYTVPSLLGPRVIGKVSAPTCS IYGRRAAGSFFEDLSKVVSPGVYKSRAPQFTIL ARTSLPQDNTRKPGPAAYNVDQHRKPRGWSFGIR HSDYLAPLVTDADN*PARRERPHTCLLKVCESA SCPPLSLSLCASWRKAWGGATAGAVSQLRTQRG GARASGEAGTPALRRVRTSRPHRGREGDSREPQT PETHAGRDRPGITPADGPGPRRPGSSACPGAPG DWPGRGVQGFPGAATAELRPPGGPTRLPSWGAA APPPAVDRCARTLNPTVPTRGRGRVPGLGSGASG RGQRPKQVRAGLKSRGGEAGGRHGRGRHRHGTE GRTPERHGKGVGTGARGSDTDAGRPQTPDVSGI PAGPERWQP**PREPGPHPRLVTSPGKGARDFPT LRQSPSSSRS*SA*SETEAA*AKRTSGASWPLW *MGARRAHRSGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARCQ*SADVVWGTQEAPWISWSLF LDSMSSRAQSRCKCCWTRRAAVSWVRVSSWFLR TESYMQPEM*QPPWTACHSSQPPFSVRNSWRGCP LWWWTLSSEGPPSSPTRSRPGSWQRRWLAWEPA *GFGSRQR*PPWTSPWVAAWATPWRWRRRCSAW TAQARQT*GTWSPRSGAPCSGSADTRGLRPRALP GWPRRWTTARPLAASSGCWRRRAWIPVWPEPCA REVPQNAGSCCLAPGSRRSCWRPQMAPWSWSGR CRWRWCCTSSGPGAAALGSRSAWGWAQSCWSTWV RGCAVGPPGSACTGTAPRSAARRAAPCRRRSYS PTARHSPPPRPSQSSFCRRSNKAPLPRNLVSAW AGAEGSRAAEAGAVSMNT*PPAGSAFRARAESLS AAAPVCACAAPGTPAPCL*ACGARFP*AGGAER RELEVGASSRAWSTDARPRREVRTPGLGGEEVA LRTRWTPG*FPSSGYCAGLAGHTPGRAGGRSEAG APVGGSQQAPVFSRPSTVITRSRGASSGPGTFL ATRALALLGRAQTQNRPGGGAVSPREGALSRRG Q*PGVLRPPPSKPSAGARGKGSKVTNAKAGRRL* ARRSCWEAWM*GGWGFVAGGSVVRLCQERLNTA GGQSLSSGGRSCRFSAGSVEEGRGWLKWLEVTG KCWRNRLLSGIASQRVTHLLLRNGFIANR*RG*F RQGMHLECFPSARGSPC*GAGAGQGAVTGW*SQ VEDRSDRCGCPGVDVWWRWSSELR*AAANAC*A *TWAGSFRWVAGLKSQGQPRHCPGPSLLAPDACA CFQEHQIHAAADSEPHSLAQALSAQASGPPWDP GRPGPASEVLAFVKAGPCRDRWAGPAPGPWASN PAADHRPVRGWTRWGLAGPEG*EGEAAAAKANRS PAPGSCGPGRLPPAGSQRPGSLQG*LPGPVGAD VLWLHSLP*HLPRRAGEAGAGGAAAGSRAWFAS SAACLHHCGPRAGRR*SHGPLRPGLPPKTVGSDR LHQTGCPG*SQLPRVLQCRPQG*GPGLHRGPLH CHLPAQP*RPLHGLLRPEQIG*ADLRQCAAAHG GFPQCPVLSHCSLGPIIKRAAF

Frame 2

QHENLLPPPHPQVIPFTKSGSRLGPREFTSLPN APGVRVMALDPLPAPSSTQPGRKLAFVLLAPGI RSRPRPAPR*SQGSGKVRSPRICGAGAAATAFC PQVLRAVSAPHPRYGLGRLGGPLAATPAPRPHRG ALRRPRAQIQAAAQHR*KGQAPGLRGGTAPGGG KGKRPGRPALGAEPCLLEGRGGLCRGRVRVADG AWGWARPDANSDAPRLRPA*PVAAARPRLHLRRA LPHAADDVRPRARPPGARSHDRARHRRRPRLLH LRPPTPLSALPHSGTWSGPPGPWPPQRRTASRE AHLGTPDLNPESPSDTLTPHIRYPHIRISNPKPR TPRGFDKSWLRLPTSPRTPSRVPTRLPRSSSPP HP*SAPQAGTSRSERGTRRTPVRLGTPLLPETG VSRRNSRAQVPRPIRCPRSWVRASSAKSPPQLAP STAAERLAVSSRTSARS*VQGSTSPGPPSSRFW RGLRSPKTTLGSQGPRPTTWISTGSPAAGVSGS GTRTTWPRW*PTRTTDPPGGSGPTRVCLKSASPH RVRLSLSLSARPGARPGVEPRLGPCPNSEPSGA GPERRARPGPQRCAASERRDPTEGGRGTLGSHR RPRPTPGGTGQGSPPPTAPGPDGPEVPRVRGHRG IGRGAACKASRGRRLPSSALQAAPPACRPGAPP PRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQA GEGSALSRYGRGSSREAGKREADTDEGDTDTGPR GGHRRDTGKGSGQEHVAQTPTPGGRRPRTCQAS PQARSDGSLDDPGNRGPTRAW*LLRGREPGTSR PFARAQAAPGADPHEARRRPPERSGHQGLRGRCG EWERAGRTDRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQGANDQRTWSGAHRRHLG*AGVYS WIQCHPEPRADASAAGPGGLLYRGSE*AAGSCG RNPICSQRCDSHRGQPATHHSLHSQ*ETRGGAV RSGGGR*VRRGRRLPQPGAGPGAGKDAGWRGSQP RASGRGSADRHGQAPGSLRGPRPGGGGGAALHG RRRPARLKGPGHHARGRPALAQRTRGDSGPGRC PGGRGAGRRLGPWPLRADAGGAGRGSRSGPSPVL GKSRRTPAAAASRPGAGGAAGARRWHRGAGPGA AAGAGAARARGRAQPRWGAAPPGGGRRAAGRRG SEAAPWDPLAPRAPGRPRAQRPAEPRPAGGARTL RPRAIRRPLALRRARSAAAAIKLLCRETLSVLG RERKDPGLRRRGPSR*TRDPRRAPPSAHALRAC QRLRPCAHAQLRGRLRPACERVAPAFPEPAGQSA GSWRSALPLVLGPLTRGPAARCGRRGWEGRR*P *GLAGLRGSFPAPATARGWRGTPQGALEAGARL GRPWEAPSRHRCSRGQAQL*RARAALRVVLEPFW PRGRWPCWGGHKPRTARAGVQ*VLGRVPSAGGG SDRES*DLHLANLLRGPVGKAQRSPTQRQGVGC EPGGAAGKPGCEEGGVLWRVEVSCVSARRG*TQP AGRV*APGVGRAGFLLGVWRKAAVG*SGWR*QE SVGGIGCSRGLQARELPTSF*EMGLLQIDNVVS SGKACTWNAFRQQEVHLAEVQVQGRVR*QAGDPR *RTGVTGVVAQVWMFGGGGVLSSGEQLQMPVKP ERGLGPSDGWLVSSRRGSPGTVLGLPFWLLTPV LVSRSIRSMLLLTRSPTAWHRLSQLKPRVLPGTL GGQALHLRSWLLSRQGPAETGGQGQPQGPGLRT RLLITGLFGAGLGGAWLALRAEKERLQQQKRTE ALRQAAVGQGDFHLLDHRGRARCKADFRGQWVLM YFGFTHCPDICPDELEKLVQVVRQLEAEPGLPP VQPVFITVDPERDDVEAMARYVQDFHPRLLGLT GSTKQVAQASHSYRVYYNAGPKDEDQDYIVDHSI AIYLLNPDGLFTDYYGRSRSAEQISDSVRRHMA AFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.shed1.aAug05

AGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGC CCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGG AGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGA GCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGC CCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGC GCAGGGCGCACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGC CCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTT CGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGG ACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAG ACGGGAGCCTATACCGCACATGCAGCAACCAGTCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

SGPLCANPEPYGPDPRARPGTWAGIRSKRARAA P*AGTGGAQVARRGSGRQTRTRATQTRDRGADTG ETRERGRDRSTWLRHRRREAADPGRVRHPRRPG AMAALMTPGTGAPPAPGDFSGEGSQGLPDPSPE PKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSA QGAQIGAWGGLGVPDPDWEVSPRDFGSLGVRRC PTTSTGPRVPHRCGLPPSRVPPHTRGHADGHPT SGHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKAS

Frame 1

VDRCARTLNPTVPTRGRGRVPGLGSGASGRGQR PKQVRAGLKSRGGEAGGRHGRGRHRHGTEGRTP ERHGKGVGTGARGSDTDAGRPQTPDVSGIPAGPE RWQP**PREPGPHPRLVTSPGKGARDFPTLRQS PSSSRS*SA*SETEAA*AKRTSGASWPLW*MGA RRAHRSVRGEGWAFLTPTGRSARETLGPWGCDGA PLPAPAPGCPTAVGCHPHAYPHIPGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLV DKHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPP

Frame 2

WTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSA LSRYGRGSSREAGKREADTDEGDTDTGPRGGHR RDTGKGSGQEHVAQTPTPGGRRPRTCQASPQAR SDGSLDDPGNRGPTRAW*LLRGREPGTSRPFARA QAAPGADPHEARRRPPERSGHQGLRGRCGEWER AGRTDRCVGRVGRS*PRLGGQPERLWVPGGATV PHYQHRPQGAPPLWAATLTRTPTYQGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFP DGSLYRTCSNQSIHRQLPTSSLAQSLQ