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Sequences

Sequence ECGF1.oAug05

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVD KHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPPRPYQGFS PPCSP

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFPD GSLYRTCSNQSIHRQLPTSSLAQSLQDPTKASP HPAP

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRGHADGHPTS GHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKASKTLPRLLP TLLP

Sequence ECGF1.gAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGC GCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGC CGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTC GGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGA CAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGT CTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGG GCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCAT CACAGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGT GGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCG GGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATC CCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGG TGAGCGTCGGGGGAGTCCCCGTCCTTCCGCCTCCGCCATCCCCTTCCCTTCCCGAGGCCCCGCCCCTTCCCGAGCCCGCGCCTCTCAGCCCCTCTCCCCG CAGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGT GGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGC CGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCT TTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCG CCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCG CTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACG CCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAG GGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGG CGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCT CGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCA CCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCG TCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGT TGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGT TTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCA GGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGG TGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGC CTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGC TCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGG CCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAG AGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGG CTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCA GCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGAC TTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGG ACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGAT CTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVD KHSTGGVGDKVSLVLAPALAACGCKVPMISGRG LGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQA GCCIVGQSEQLVPADGILYAARDVTATVDSLPLI TGWRGSQPRASGRGSADRHGQAPGSLRGPRPGG GGGAALHGRRRPARLKGPGHHARGRPALAQRTR GDSGPGRCPGGRGAGRRLGPWPLRADAGGAGRGS RSGPSPVLGKSRRTPAAAASRPGAGGAAGARRW *ASGESPSFRLRHPLPFPRPRPFPSPRLSAPLP AGTVELVRALPLALVLHELGAGRSRAGEPLRLGV GAELLVDVGQRLRRGTPWLRVHRDGPALSGPQS RALQEALVLSDRAPFAAPSPFAELVLPPQQ*SS FAAKPCQCLGGSGRIQGCGGGGRLDEHVTPGGLR LPRTR*EPVSGCARVRMRSSGDACALPVSVWRP LSLSRRGRAQGAGGRRFLSCLVH*RAAPPRGAD AGAGRGGGSPEDSLDSGVVSQLRLLRGAGGAHPR ARWRPERGWGARGRLPAGTGVLAAKHSYNALAR RFEWSWNLSGHEGAGLAGEGTNPEPPGRGCSES SGGCPQQEGAVTGSPETST*QTFCGGPWERLKGH QRKGRASAVSPEELLGSLDVRRVGFCGGWKCRA SLPGEVKHSRRAESELRG*VVQVFCWECGGRPR LVEVAGGNRKVLEESVALGDCKPESYPPPFKKWV YCK*ITWLVQARHALGMLSVSKRFTLLRCRCRA GCGDRLVIPGRGQE*QVWLPRCGCLVEVEF*AQ VSSCKCLLSLNVGWVLQMGGWSQVAGAAQALSWA FPSGS*RLCLFPGASDPCCC*LGAPQLGTGSLS SSLGSSLGPWEARPCI*GPGFCQGRALQRQVGR ASPRALGFEPGC*SQACSGLDSVGPGWP*GLRRR GCSSKSEQKPCARQLWARATSTCWITEAGLAAR LTSGASGC*CTLASLTALTSAQTSWRSWCRWCG SWKQSLVCLQCSLSSSLWTPSGTTLKPWPATSRT STQDCWV*PAPPNRLPRLVTVTACTTMQAPRMR TRTTSWTTPLPSTCSTLTASSRITTAGADRLSR SQTVCGGTWRLSAVSCLEPLQSGPHH*TGCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARCQ*SADVV WGTQEAPWISWSLFLDSMSSRAQSRCKCCWTRR AAVSWVRVSSWFLRTESYMQPEM*QPPWTACHS SQVGVGASLGLRVAAALTAMDKPLGRCVGHALEV EEALLCMDGAGPPDLRDLVTTLGGALLWLSGHA GTQAQGAARVAAALDDGSALGRFERMLAAQGVD PGLARALCSGSPAERRQLLPRAREQEELLAPADG ERRGSPRPSASAIPFPSRGPAPSRARASQPLSP QAPWSWSGRCRWRWCCTSSGPGAAALGSRSAWG WAQSCWSTWVRGCAVGPPGSACTGTAPRSAARRA APCRRRSYSPTARHSPPPRPSQSSFCRRSNKAP LPRNLVSAWAGAEGSRAAEAGAVSMNT*PPAGS AFRARAESLSAAAPVCACAAPGTPAPCL*ACGAR FP*AGGAERRELEVGASSRAWSTDARPRREVRT PGLGGEEVALRTRWTPG*FPSSGYCAGLAGHTP GRAGGRSEAGAPVGGSQQAPVFSRPSTVITRSRG ASSGPGTFLATRALALLGRAQTQNRPGGGAVSP REGALSRRGQ*PGVLRPPPSKPSAGARGKGSKV TNAKAGRRL*ARRSCWEAWM*GGWGFVAGGSVVR LCQERLNTAGGQSLSSGGRSCRFSAGSVEEGRG WLKWLEVTGKCWRNRLLSGIASQRVTHLLLRNG FIANR*RG*FRQGMHLECFPSARGSPC*GAGAGQ GAVTGW*SQVEDRSDRCGCPGVDVWWRWSSELR *AAANAC*A*TWAGSFRWVAGLKSQGQPRHCPG PSLLAPDACACFQEHQIHAAADSEPHSLAQALSA QASGPPWDPGRPGPASEVLAFVKAGPCRDRWAG PAPGPWASNPAADHRPVRGWTRWGLAGPEG*EG EAAAAKANRSPAPGSCGPGRLPPAGSQRPGSLQG *LPGPVGADVLWLHSLP*HLPRRAGEAGAGGAA AGSRAWFASSAACLHHCGPRAGRR*SHGPLRPG LPPKTVGSDRLHQTGCPG*SQLPRVLQCRPQG*G PGLHRGPLHCHLPAQP*RPLHGLLRPEQIG*AD LRQCAAAHGGFPQCPVLSHCSLGPIIKRAAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRGHADGHPTS GHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQGANDQRTW SGAHRRHLG*AGVYSWIQCHPEPRADASAAGPGG LLYRGSE*AAGSCGRNPICSQRCDSHRGQPATH HRLAWEPA*GFGSRQR*PPWTSPWVAAWATPWR WRRRCSAWTAQARQT*GTWSPRSGAPCSGSADTR GLRPRALPGWPRRWTTARPLAASSGCWRRRAWI PVWPEPCAREVPQNAGSCCLAPGSRRSCWRPQM VSVGGVPVLPPPPSPSLPEAPPLPEPAPLSPSPR RHRGAGPGAAAGAGAARARGRAQPRWGAAPPGG GRRAAGRRGSEAAPWDPLAPRAPGRPRAQRPAE PRPAGGARTLRPRAIRRPLALRRARSAAAAIKLL CRETLSVLGRERKDPGLRRRGPSR*TRDPRRAP PSAHALRACQRLRPCAHAQLRGRLRPACERVAP AFPEPAGQSAGSWRSALPLVLGPLTRGPAARCGR RGWEGRR*P*GLAGLRGSFPAPATARGWRGTPQ GALEAGARLGRPWEAPSRHRCSRGQAQL*RARA ALRVVLEPFWPRGRWPCWGGHKPRTARAGVQ*VL GRVPSAGGGSDRES*DLHLANLLRGPVGKAQRS PTQRQGVGCEPGGAAGKPGCEEGGVLWRVEVSC VSARRG*TQPAGRV*APGVGRAGFLLGVWRKAAV G*SGWR*QESVGGIGCSRGLQARELPTSF*EMG LLQIDNVVSSGKACTWNAFRQQEVHLAEVQVQG RVR*QAGDPR*RTGVTGVVAQVWMFGGGGVLSSG EQLQMPVKPERGLGPSDGWLVSSRRGSPGTVLG LPFWLLTPVLVSRSIRSMLLLTRSPTAWHRLSQ LKPRVLPGTLGGQALHLRSWLLSRQGPAETGGQG QPQGPGLRTRLLITGLFGAGLGGAWLALRAEKE RLQQQKRTEALRQAAVGQGDFHLLDHRGRARCK ADFRGQWVLMYFGFTHCPDICPDELEKLVQVVRQ LEAEPGLPPVQPVFITVDPERDDVEAMARYVQD FHPRLLGLTGSTKQVAQASHSYRVYYNAGPKDE DQDYIVDHSIAIYLLNPDGLFTDYYGRSRSAEQI SDSVRRHMAAFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.eAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGC GCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGC CGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTC GGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGA CAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGATGCAAGTGCTGCTGGACCAGGCG GGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCA TCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCA GGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGC GTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGC TCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCT GGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAG CTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGC CGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCT CAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCG CAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGA CCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGT GAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCG CCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTG GCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTA TAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGG GGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGG AAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGG GTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGT GGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTC CTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAG GTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTG GAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCC AGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGG CACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAG AGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCT GAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGC CGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGG TGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGC CCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCA GGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCA GATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGG GCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVD KHSTGGVGDKVSLVLAPALAACGCKMQVLLDQA GCCIVGQSEQLVPADGILYAARDVTATVDSLPL ITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQ ARELAKTLVGVGASLGLRVAAALTAMDKPLGRC VGHALEVEEALLCMDGAGPPDLRDLVTTLGGAL LWLSGHAGTQAQGAARVAAALDDGSALGRFERML AAQGVDPGLARALCSGSPAERRQLLPRAREQEE LLAPADGTVELVRALPLALVLHELGAGRSRAGE PLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPAL SGPQSRALQEALVLSDRAPFAAPSPFAELVLPP QQ*SSFAAKPCQCLGGSGRIQGCGGGGRLDEHV TPGGLRLPRTR*EPVSGCARVRMRSSGDACALPV SVWRPLSLSRRGRAQGAGGRRFLSCLVH*RAAP PRGADAGAGRGGGSPEDSLDSGVVSQLRLLRGA GGAHPRARWRPERGWGARGRLPAGTGVLAAKHSY NALARRFEWSWNLSGHEGAGLAGEGTNPEPPGR GCSESSGGCPQQEGAVTGSPETST*QTFCGGPW ERLKGHQRKGRASAVSPEELLGSLDVRRVGFCGG WKCRASLPGEVKHSRRAESELRG*VVQVFCWEC GGRPRLVEVAGGNRKVLEESVALGDCKPESYPP PFKKWVYCK*ITWLVQARHALGMLSVSKRFTLLR CRCRAGCGDRLVIPGRGQE*QVWLPRCGCLVEV EF*AQVSSCKCLLSLNVGWVLQMGGWSQVAGAA QALSWAFPSGS*RLCLFPGASDPCCC*LGAPQLG TGSLSSSLGSSLGPWEARPCI*GPGFCQGRALQ RQVGRASPRALGFEPGC*SQACSGLDSVGPGWP *GLRRRGCSSKSEQKPCARQLWARATSTCWITEA GLAARLTSGASGC*CTLASLTALTSAQTSWRSW CRWCGSWKQSLVCLQCSLSSSLWTPSGTTLKPW PATSRTSTQDCWV*PAPPNRLPRLVTVTACTTMQ APRMRTRTTSWTTPLPSTCSTLTASSRITTAGA DRLSRSQTVCGGTWRLSAVSCLEPLQSGPHH*T GCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARCKCCWTRR AAVSWVRVSSWFLRTESYMQPEM*QPPWTACHS SQPPFSVRNSWRGCPLWWWTLSSEGPPSSPTRS RPGSWQRRWLAWEPA*GFGSRQR*PPWTSPWVAA WATPWRWRRRCSAWTAQARQT*GTWSPRSGAPC SGSADTRGLRPRALPGWPRRWTTARPLAASSGC WRRRAWIPVWPEPCAREVPQNAGSCCLAPGSRRS CWRPQMAPWSWSGRCRWRWCCTSSGPGAAALGS RSAWGWAQSCWSTWVRGCAVGPPGSACTGTAPR SAARRAAPCRRRSYSPTARHSPPPRPSQSSFCRR SNKAPLPRNLVSAWAGAEGSRAAEAGAVSMNT* PPAGSAFRARAESLSAAAPVCACAAPGTPAPCL *ACGARFP*AGGAERRELEVGASSRAWSTDARPR REVRTPGLGGEEVALRTRWTPG*FPSSGYCAGL AGHTPGRAGGRSEAGAPVGGSQQAPVFSRPSTV ITRSRGASSGPGTFLATRALALLGRAQTQNRPGG GAVSPREGALSRRGQ*PGVLRPPPSKPSAGARG KGSKVTNAKAGRRL*ARRSCWEAWM*GGWGFVA GGSVVRLCQERLNTAGGQSLSSGGRSCRFSAGSV EEGRGWLKWLEVTGKCWRNRLLSGIASQRVTHL LLRNGFIANR*RG*FRQGMHLECFPSARGSPC* GAGAGQGAVTGW*SQVEDRSDRCGCPGVDVWWRW SSELR*AAANAC*A*TWAGSFRWVAGLKSQGQP RHCPGPSLLAPDACACFQEHQIHAAADSEPHSL AQALSAQASGPPWDPGRPGPASEVLAFVKAGPCR DRWAGPAPGPWASNPAADHRPVRGWTRWGLAGP EG*EGEAAAAKANRSPAPGSCGPGRLPPAGSQR PGSLQG*LPGPVGADVLWLHSLP*HLPRRAGEAG AGGAAAGSRAWFASSAACLHHCGPRAGRR*SHG PLRPGLPPKTVGSDRLHQTGCPG*SQLPRVLQC RPQG*GPGLHRGPLHCHLPAQP*RPLHGLLRPEQ IG*ADLRQCAAAHGGFPQCPVLSHCSLGPIIKR AAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRGHADGHPTS GHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQDASAAGPG GLLYRGSE*AAGSCGRNPICSQRCDSHRGQPATH HSLHSQ*ETRGGAVRSGGGR*VRRGRRLPQPGA GPGAGKDAGWRGSQPRASGRGSADRHGQAPGSL RGPRPGGGGGAALHGRRRPARLKGPGHHARGRPA LAQRTRGDSGPGRCPGGRGAGRRLGPWPLRADA GGAGRGSRSGPSPVLGKSRRTPAAAASRPGAGG AAGARRWHRGAGPGAAAGAGAARARGRAQPRWGA APPGGGRRAAGRRGSEAAPWDPLAPRAPGRPRA QRPAEPRPAGGARTLRPRAIRRPLALRRARSAA AAIKLLCRETLSVLGRERKDPGLRRRGPSR*TRD PRRAPPSAHALRACQRLRPCAHAQLRGRLRPAC ERVAPAFPEPAGQSAGSWRSALPLVLGPLTRGP AARCGRRGWEGRR*P*GLAGLRGSFPAPATARGW RGTPQGALEAGARLGRPWEAPSRHRCSRGQAQL *RARAALRVVLEPFWPRGRWPCWGGHKPRTARA GVQ*VLGRVPSAGGGSDRES*DLHLANLLRGPVG KAQRSPTQRQGVGCEPGGAAGKPGCEEGGVLWR VEVSCVSARRG*TQPAGRV*APGVGRAGFLLGV WRKAAVG*SGWR*QESVGGIGCSRGLQARELPTS F*EMGLLQIDNVVSSGKACTWNAFRQQEVHLAE VQVQGRVR*QAGDPR*RTGVTGVVAQVWMFGGG GVLSSGEQLQMPVKPERGLGPSDGWLVSSRRGSP GTVLGLPFWLLTPVLVSRSIRSMLLLTRSPTAW HRLSQLKPRVLPGTLGGQALHLRSWLLSRQGPA ETGGQGQPQGPGLRTRLLITGLFGAGLGGAWLAL RAEKERLQQQKRTEALRQAAVGQGDFHLLDHRG RARCKADFRGQWVLMYFGFTHCPDICPDELEKL VQVVRQLEAEPGLPPVQPVFITVDPERDDVEAMA RYVQDFHPRLLGLTGSTKQVAQASHSYRVYYNA GPKDEDQDYIVDHSIAIYLLNPDGLFTDYYGRS RSAEQISDSVRRHMAAFRSVLS*ATAVWAPSLNG LRL

Sequence ECGF1.dAug05

TTCTTTTTTTTACATTTTGTTTGCCTAGAATTGTATTTGATTGTAATCAAATCATGTGTCACATCTTTTTTTCAATTTGTGTGATTATTTTGTGGCCCAA TACATGATTTTTTTTGCGAATATTTCATGGACTTAAATAGATAAGTCCTCTCTCTACACATAATTAAATGGAGTGTGTGCTTTGCATTATTCAGTTCCTT TATTCCCATCCTTGCTTTTGTCTGCAGATTTGTCCTATTCCAGAATGGGTATACTGAAACTTGGGTAAGGGTGAGGCCAGCGTTCTGGGTGGCCCAGGCT ACTATCCCACCCGTGCCACAGAATCCACACAGAGGCCCAGCGGCGGCACTGGGTGGTGGGGAAGGGGGAGGCGGACCCAGGTTGGGGGGGCAGGCAGTCA GTGCGGTTGCCAGGAAGCGGGAAAGGGCCACAATGGGGTCTGGGAGGTGGGCGGGGCGGAGCGGGGATGTCCAGCCACGTCGCTTTGTTTTCCCACGCTA GGAGCTACCACAACAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGG CCCCATCGCGGCGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCC TTCACCTTCGGCGCGCGCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCG CCCCCGCCTACTCCATCTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTA CCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGGTGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTG GGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCCATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGACCCCGGGCC CCTGCGCCTATCAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGGCGCGGACTTCGCTCCCCCAAGACAACACTCGGAA GCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCGGCACTCGGACTACCTGGCCCCGCTGGTGACCGAC GCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCATCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGT CCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCGGGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGC GCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGACGCCCGAGACCCACGCCGGGCGGGACCGGCCAGGG ATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGGGATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGG GGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAAC CCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCCGGAGCGAT GGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAG CAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGG GCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCA GCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCG GCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCC AGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGC CAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTT AAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGG CAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGA CTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTG GACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAAC GCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCT GCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGG ACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCG CCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCA GGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTG CGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGC GCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTC CGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTC CCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCC TTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGA GACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTG GGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGC TCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGT TGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTT GGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGAC AGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCT TCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCA GATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCA TCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGC CTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTG TGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCA CTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATC ACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTG CCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAA CCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTG TCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

FFFLHFVCLELYLIVIKSCVTSFFQFV*LFCGP IHDFFCEYFMDLNR*VLSLHIIKWSVCFALFSSF IPILAFVCRFVLFQNGYTETWVRVRPAFWVAQA TIPPVPQNPHRGPAAALGGGEGGGGPRLGGQAV SAVARKRERATMGSGRWAGRSGDVQPRRFVFPR* ELPQQVLRAVSAPHPRYGLGRLGGPLAATPAPR PHRGALRRPRAQIQAAAQHRLRPA*PVAAARPR LHLRRALPHAADDVRPRARPPGARSHDRARHRRR PRLLHLRPPTPLSALPHSGTWQVLPGASGERDV PQCASAHHCSPKLGCPGGTAEPRSRGLYGALAL GSARHRQSLRPNLLHLRPQSGWQFLRGPQQDPGP LRLSGRESRGLQVPGPPVHDSGADFAPPRQHSE ARARGLQRGSAPEAPRLEFRDPALGLPGPAGDR RGQLTRQAGAAPHVFA*SLRVRIVSASLSLSLRV LAQGLGWSHGWGRVPTPNPAGRGPSVGRGRDPS AAPRPNVETPPRAGGGLSGATDARDPRRAGPAR DHPRRRPRAPTARKFRVSGGTGGLAGARRARLPG GGDCRAPPSRRPHPPAVLGRRRPAAGSGPLCAN PEPYGPDPRARPGTWAGIRSKRARAAP*AGPER WQP**PREPGPHPRLVTSPGKGARDFPTLRQSPS SSRS*SA*SETEAA*AKRTSGASWPLW*MGARR AHRSGPC*WPSDFGAWIWRRPRC*PRPWLSRDS SWSGQRPGASSLWTSIPQGVWVTRSAWSSHLPWR HVAARCQ*SADVVWGTQEAPWISWSLFLDSMSS RAQSRCKCCWTRRAAVSWVRVSSWFLRTESYMQ PEM*QPPWTACHSSQPPFSVRNSWRGCPLWWWTL SSEGPPSSPTRSRPGSWQRRWLAWEPA*GFGSR QR*PPWTSPWVAAWATPWRWRRRCSAWTAQARQ T*GTWSPRSGAPCSGSADTRGLRPRALPGWPRRW TTARPLAASSGCWRRRAWIPVWPEPCAREVPQN AGSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWC CTSSGPGAAALGSRSAWGWAQSCWSTWVRGCAVG PPGSACTGTAPRSAARRAAPCRRRSYSPTARHS PPPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGS RAAEAGAVSMNT*PPAGSAFRARAESLSAAAPVC ACAAPGTPAPCL*ACGARFP*AGGAERRELEVG ASSRAWSTDARPRREVRTPGLGGEEVALRTRWT PG*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGGS QQAPVFSRPSTVITRSRGASSGPGTFLATRALA LLGRAQTQNRPGGGAVSPREGALSRRGQ*PGVL RPPPSKPSAGARGKGSKVTNAKAGRRL*ARRSCW EAWM*GGWGFVAGGSVVRLCQERLNTAGGQSLS SGGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNR LLSGIASQRVTHLLLRNGFIANR*RG*FRQGMHL ECFPSARGSPC*GAGAGQGAVTGW*SQVEDRSD RCGCPGVDVWWRWSSELR*AAANAC*A*TWAGS FRWVAGLKSQGQPRHCPGPSLLAPDACACFQEHQ IHAAADSEPHSLAQALSAQASGPPWDPGRPGPA SEVLAFVKAGPCRDRWAGPAPGPWASNPAADHR PVRGWTRWGLAGPEG*EGEAAAAKANRSPAPGSC GPGRLPPAGSQRPGSLQG*LPGPVGADVLWLHS LP*HLPRRAGEAGAGGAAAGSRAWFASSAACLH HCGPRAGRR*SHGPLRPGLPPKTVGSDRLHQTGC PG*SQLPRVLQCRPQG*GPGLHRGPLHCHLPAQ P*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCP VLSHCSLGPIIKRAAF

Frame 1

SFFYILFA*NCI*L*SNHVSHLFFNLCDYFVAQ YMIFFANISWT*IDKSSLYT*LNGVCALHYSVP LFPSLLLSADLSYSRMGILKLG*G*GQRSGWPRL LSHPCHRIHTEAQRRHWVVGKGEADPGWGGRQS VRLPGSGKGPQWGLGGGRGGAGMSSHVALFSHA RSYHNRCCEP*APPTRAMGSDAWVGLWRPHRPRG PIAAHYGGPGPKYKLPPNTGYALHDPSRPRAPA FTFGARFPTQQTTCGPGPGHLVPARMTVRGTDG APAYSIYGRPRRSAPFLTPGPGRYFPERAGNATY PSAPRHTIAPRNWGVQAEQQSPGPAAYTVPSLL GPRVIGKVSAPTCSIYGRRAAGSFFEDLSKTPG PCAYQVVSPGVYKSRAPQFTILARTSLPQDNTRK PGPAAYNVDQHRKPRGWSFGIRHSDYLAPLVTD ADN*PARRERPHTCLLKVCESASCPPLSLSLCA SWRKAWGGATAGAVSQLRTQRGGARASGEAGTPA LRRVRTSRPHRGREGDSREPQTPETHAGRDRPG ITPADGPGPRRPGSSACPGAPGDWPGRGVQGFP GAATAELRPPGGPTRLPSWGAAAPPPAVDRCART LNPTVPTRGRGRVPGLGSGASGRGQRPKQARSD GSLDDPGNRGPTRAW*LLRGREPGTSRPFARAQ AAPGADPHEARRRPPERSGHQGLRGRCGEWERAG RTDRGHADGHPTSGHGSGGDLGADPGPGSVGTA AGVARGLAPAACGQAFHRGCG*QGQPGPRTCPG GMWLQGANDQRTWSGAHRRHLG*AGVYSWIQCHP EPRADASAAGPGGLLYRGSE*AAGSCGRNPICS QRCDSHRGQPATHHSLHSQ*ETRGGAVRSGGGR *VRRGRRLPQPGAGPGAGKDAGWRGSQPRASGRG SADRHGQAPGSLRGPRPGGGGGAALHGRRRPAR LKGPGHHARGRPALAQRTRGDSGPGRCPGGRGA GRRLGPWPLRADAGGAGRGSRSGPSPVLGKSRRT PAAAASRPGAGGAAGARRWHRGAGPGAAAGAGA ARARGRAQPRWGAAPPGGGRRAAGRRGSEAAPW DPLAPRAPGRPRAQRPAEPRPAGGARTLRPRAIR RPLALRRARSAAAAIKLLCRETLSVLGRERKDP GLRRRGPSR*TRDPRRAPPSAHALRACQRLRPC AHAQLRGRLRPACERVAPAFPEPAGQSAGSWRSA LPLVLGPLTRGPAARCGRRGWEGRR*P*GLAGL RGSFPAPATARGWRGTPQGALEAGARLGRPWEA PSRHRCSRGQAQL*RARAALRVVLEPFWPRGRWP CWGGHKPRTARAGVQ*VLGRVPSAGGGSDRES* DLHLANLLRGPVGKAQRSPTQRQGVGCEPGGAA GKPGCEEGGVLWRVEVSCVSARRG*TQPAGRV*A PGVGRAGFLLGVWRKAAVG*SGWR*QESVGGIG CSRGLQARELPTSF*EMGLLQIDNVVSSGKACT WNAFRQQEVHLAEVQVQGRVR*QAGDPR*RTGVT GVVAQVWMFGGGGVLSSGEQLQMPVKPERGLGP SDGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSI RSMLLLTRSPTAWHRLSQLKPRVLPGTLGGQALH LRSWLLSRQGPAETGGQGQPQGPGLRTRLLITG LFGAGLGGAWLALRAEKERLQQQKRTEALRQAA VGQGDFHLLDHRGRARCKADFRGQWVLMYFGFTH CPDICPDELEKLVQVVRQLEAEPGLPPVQPVFI TVDPERDDVEAMARYVQDFHPRLLGLTGSTKQV AQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLN PDGLFTDYYGRSRSAEQISDSVRRHMAAFRSVL S*ATAVWAPSLNGLRL

Frame 2

LFFTFCLPRIVFDCNQIMCHIFFSICVIILWPN T*FFLRIFHGLK*ISPLSTHN*MECVLCIIQFL YSHPCFCLQICPIPEWVY*NLGKGEASVLGGPG YYPTRATESTQRPSGGTGWWGRGRRTQVGGAGSQ CGCQEAGKGHNGVWEVGGAERGCPATSLCFPTL GATTTGAASRKRPPPALWARTPGWAFGGHTGPA APSRRTTEAPGPNTSCRPTPATPCMTRRGRAPPP SPSARASPRSRRRAAPGQATWCPLA*PCAAPTA PPPTPSTAAHAAQRPSSLRDLAGTSRSERGTRR TPVRLGTPLLPETGVSRRNSRAQVPRPIRCPRSW VRASSAKSPPQLAPSTAAERLAVSSRTSARPRA PAPIRS*VQGSTSPGPPSSRFWRGLRSPKTTLG SQGPRPTTWISTGSPAAGVSGSGTRTTWPRW*PT RTTDPPGGSGPTRVCLKSASPHRVRLSLSLSAR PGARPGVEPRLGPCPNSEPSGAGPERRARPGPQ RCAASERRDPTEGGRGTLGSHRRPRPTPGGTGQG SPPPTAPGPDGPEVPRVRGHRGIGRGAACKASR GRRLPSSALQAAPPACRPGAPPPRRRQWTAVRE P*TLRSRPAGEAGYLGWDPEQAGEGSALSRPGAM AALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPK QLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQ GAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQ LEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALA ACGCKVPMISGRGLGHTGGTLDKLESIPGFNVI QSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAA RDVTATVDSLPLITASILSKKLVEGLSALVVDV KFGGAAVFPNQEQARELAKTLVGVGASLGLRVA AALTAMDKPLGRCVGHALEVEEALLCMDGAGPPD LRDLVTTLGGALLWLSGHAGTQAQGAARVAAAL DDGSALGRFERMLAAQGVDPGLARALCSGSPAE RRQLLPRAREQEELLAPADGTVELVRALPLALVL HELGAGRSRAGEPLRLGVGAELLVDVGQRLRRG TPWLRVHRDGPALSGPQSRALQEALVLSDRAPF AAPSPFAELVLPPQQ*SSFAAKPCQCLGGSGRIQ GCGGGGRLDEHVTPGGLRLPRTR*EPVSGCARV RMRSSGDACALPVSVWRPLSLSRRGRAQGAGGR RFLSCLVH*RAAPPRGADAGAGRGGGSPEDSLDS GVVSQLRLLRGAGGAHPRARWRPERGWGARGRL PAGTGVLAAKHSYNALARRFEWSWNLSGHEGAG LAGEGTNPEPPGRGCSESSGGCPQQEGAVTGSPE TST*QTFCGGPWERLKGHQRKGRASAVSPEELL GSLDVRRVGFCGGWKCRASLPGEVKHSRRAESE LRG*VVQVFCWECGGRPRLVEVAGGNRKVLEESV ALGDCKPESYPPPFKKWVYCK*ITWLVQARHAL GMLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE* QVWLPRCGCLVEVEF*AQVSSCKCLLSLNVGWVL QMGGWSQVAGAAQALSWAFPSGS*RLCLFPGAS DPCCC*LGAPQLGTGSLSSSLGSSLGPWEARPC I*GPGFCQGRALQRQVGRASPRALGFEPGC*SQA CSGLDSVGPGWP*GLRRRGCSSKSEQKPCARQL WARATSTCWITEAGLAARLTSGASGC*CTLASL TALTSAQTSWRSWCRWCGSWKQSLVCLQCSLSSS LWTPSGTTLKPWPATSRTSTQDCWV*PAPPNRL PRLVTVTACTTMQAPRMRTRTTSWTTPLPSTCS TLTASSRITTAGADRLSRSQTVCGGTWRLSAVSC LEPLQSGPHH*TGCV*

Sequence ECGF1.sAug05

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRPGAMAALMTPGTGAPPA PGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGG RLSEADIRGFVAAVVNGSAQGAQIGAWGGLGVP DPDWEVSPRDFGSLGVRRCPTTSTGPRVPHRCGL PPSRVPPHTRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQG*KPPPFQTGAYTAHAATSPSTG SSQPQAWPKAS

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGPERWQP**PREPGPHP RLVTSPGKGARDFPTLRQSPSSSRS*SA*SETEA A*AKRTSGASWPLW*MGARRAHRSVRGEGWAFL TPTGRSARETLGPWGCDGAPLPAPAPGCPTAVG CHPHAYPHIPGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVRNHLLSRREPIPHMQQPVHPQ AAPNLKPGPKPP

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQARSDGSLDDPGNRGPTR AW*LLRGREPGTSRPFARAQAAPGADPHEARRR PPERSGHQGLRGRCGEWERAGRTDRCVGRVGRS* PRLGGQPERLWVPGGATVPHYQHRPQGAPPLWA ATLTRTPTYQGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARLETTSFPDGSLYRTCSNQSIHR QLPTSSLAQSLQ

Sequence ECGF1.aAug05

AACAGCACGAAAATCTCCTGCCCCCACCCCACCCCCAGGTGATTCCCTTCACCAAGTCTGGGTCTAGACTTGGCCCCCGCGAGTTCACGTCCCTGCCTAA TGCGCCTGGAGTCCGGGTGATGGCCCTGGATCCGCTCCCGGCCCCAAGCTCCACACAGCCGGGAAGGAAGCTCGCGTTTGTTTTGCTGGCGCCAGGGATA CGGTCCAGGCCCCGCCCCGCCCCGCGCTGATCCCAGGGTAGTGGGAAAGTGCGCTCCCCAAGAATTTGCGGTGCAGGGGCCGCGGCCACCGCCTTCTGTC CGCAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGGCCCCATCGCGG CGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGTAGAAGGGGCAGGCCCCGGGATTGAGGGGTGGGACGGCGCCTGGGGGAGGG AAGGGGAAGAGACCAGGGAGGCCTGCGCTCGGGGCTGAACCGTGCCTGCTAGAGGGGAGAGGGGGTCTGTGCCGGGGGAGGGTCCGTGTTGCAGATGGGG CTTGGGGCTGGGCCCGGCCAGACGCTAACTCGGATGCTCCCAGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCCTTCACCTTCGGCGCGC GCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCGCCCCCGCCTACTCCAT CTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGTCAGGACCCCCGGGCCCCTGGCCACCCCAACGCCGAACTGCCTCCAGGGAGG CCCACCTGGGAACCCCCGACCTGAACCCCGAGTCCCCCTCGGATACCCTAACACCGCATATTCGGTACCCCCATATCCGGATCTCAAATCCCAAACCCCG AACCCCACGGGGCTTTGATAAATCGTGGCTCAGACTCCCCACTAGTCCCAGGACCCCATCTCGGGTACCCACCAGGCTCCCACGCAGTTCTAGCCCCCCA CACCCTTGATCCGCCCCGCAGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTACCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGG TGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTGGGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCC ATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGG CGCGGACTTCGCTCCCCCAAGACAACACTCGGAAGCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCG GCACTCGGACTACCTGGCCCCGCTGGTGACCGACGCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCA TCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGTCCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCG GGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGCGCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGAC GCCCGAGACCCACGCCGGGCGGGACCGGCCAGGGATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGG GATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGGGGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCG CCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGG GCGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAG GGGCGGACACCGGAGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCC CCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGAC CCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGG TGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCT GGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTC CTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTC CTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGA CGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCC GCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCC TAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGA CGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCC CGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCT CGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCT GCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGT CAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCT CCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGG CGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTC AGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGC AGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCC TGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGG GGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTG GCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGC AGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTG AGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCC GGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAA AGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTT CAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAG GTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCT GAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGC TTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCT GGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACC CGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAG CCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGAT GTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCA GTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCG GCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCAT TGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCG GCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

NSTKISCPHPTPR*FPSPSLGLDLAPASSRPCL MRLESG*WPWIRSRPQAPHSREGSSRLFCWRQGY GPGPAPPRADPRVVGKCAPQEFAVQGPRPPPSV RRCCEP*APPTRAMGSDAWVGLWRPHRPRGPIA AHYGGPGPKYKLPPNTGRRGRPRD*GVGRRLGEG RGRDQGGLRSGLNRAC*RGEGVCAGGGSVLQMG LGAGPGQTLTRMLPGYALHDPSRPRAPAFTFGA RFPTQQTTCGPGPGHLVPARMTVRGTDGAPAYSI YGRPRRSAPFLTPGPGQDPRAPGHPNAELPPGR PTWEPPT*TPSPPRIP*HRIFGTPISGSQIPNP EPHGALINRGSDSPLVPGPHLGYPPGSHAVLAPH TLDPPRRQVLPGASGERDVPQCASAHHCSPKLG CPGGTAEPRSRGLYGALALGSARHRQSLRPNLL HLRPQSGWQFLRGPQQGRESRGLQVPGPPVHDSG ADFAPPRQHSEARARGLQRGSAPEAPRLEFRDP ALGLPGPAGDRRGQLTRQAGAAPHVFA*SLRVR IVSASLSLSLRVLAQGLGWSHGWGRVPTPNPAGR GPSVGRGRDPSAAPRPNVETPPRAGGGLSGATD ARDPRRAGPARDHPRRRPRAPTARKFRVSGGTG GLAGARRARLPGGGDCRAPPSRRPHPPAVLGRRR PAAGSGPLCANPEPYGPDPRARPGTWAGIRSKR ARAAP*AGTGGAQVARRGSGRQTRTRATQTRDR GADTGETRERGRDRSTWLRHRRREAADPGRVRHP RRPGAMAALMTPGTGAPPAPGDFSGEGSQGLPD PSPEPKQLPELIRMKRDGGRLSEADIRGFVAAV VNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVPMISGRGLGHTGGTLDKLESI PGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAD GILYAARDVTATVDSLPLITASILSKKLVEGLS ALVVDVKFGGAAVFPNQEQARELAKTLVGVGAS LGLRVAAALTAMDKPLGRCVGHALEVEEALLCMD GAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAA RVAAALDDGSALGRFERMLAAQGVDPGLARALC SGSPAERRQLLPRAREQEELLAPADGTVELVRAL PLALVLHELGAGRSRAGEPLRLGVGAELLVDVG QRLRRGTPWLRVHRDGPALSGPQSRALQEALVL SDRAPFAAPSPFAELVLPPQQ*SSFAAKPCQCLG GSGRIQGCGGGGRLDEHVTPGGLRLPRTR*EPV SGCARVRMRSSGDACALPVSVWRPLSLSRRGRA QGAGGRRFLSCLVH*RAAPPRGADAGAGRGGGSP EDSLDSGVVSQLRLLRGAGGAHPRARWRPERGW GARGRLPAGTGVLAAKHSYNALARRFEWSWNLS GHEGAGLAGEGTNPEPPGRGCSESSGGCPQQEGA VTGSPETST*QTFCGGPWERLKGHQRKGRASAV SPEELLGSLDVRRVGFCGGWKCRASLPGEVKHS RRAESELRG*VVQVFCWECGGRPRLVEVAGGNRK VLEESVALGDCKPESYPPPFKKWVYCK*ITWLV QARHALGMLSVSKRFTLLRCRCRAGCGDRLVIP GRGQE*QVWLPRCGCLVEVEF*AQVSSCKCLLSL NVGWVLQMGGWSQVAGAAQALSWAFPSGS*RLC LFPGASDPCCC*LGAPQLGTGSLSSSLGSSLGP WEARPCI*GPGFCQGRALQRQVGRASPRALGFEP GC*SQACSGLDSVGPGWP*GLRRRGCSSKSEQK PCARQLWARATSTCWITEAGLAARLTSGASGC* CTLASLTALTSAQTSWRSWCRWCGSWKQSLVCLQ CSLSSSLWTPSGTTLKPWPATSRTSTQDCWV*P APPNRLPRLVTVTACTTMQAPRMRTRTTSWTTP LPSTCSTLTASSRITTAGADRLSRSQTVCGGTWR LSAVSCLEPLQSGPHH*TGCV*

Frame 1

TARKSPAPTPPPGDSLHQVWV*TWPPRVHVPA* CAWSPGDGPGSAPGPKLHTAGKEARVCFAGARD TVQAPPRPALIPG*WESALPKNLRCRGRGHRLLS AGAASRKRPPPALWARTPGWAFGGHTGPAAPSR RTTEAPGPNTSCRPTPVEGAGPGIEGWDGAWGR EGEETREACARG*TVPARGERGSVPGEGPCCRWG LGLGPARR*LGCSQATPCMTRRGRAPPPSPSAR ASPRSRRRAAPGQATWCPLA*PCAAPTAPPPTP STAAHAAQRPSSLRDLVRTPGPLATPTPNCLQGG PPGNPRPEPRVPLGYPNTAYSVPPYPDLKSQTP NPTGL**IVAQTPH*SQDPISGTHQAPTQF*PP TPLIRPAGRYFPERAGNATYPSAPRHTIAPRNWG VQAEQQSPGPAAYTVPSLLGPRVIGKVSAPTCS IYGRRAAGSFFEDLSKVVSPGVYKSRAPQFTIL ARTSLPQDNTRKPGPAAYNVDQHRKPRGWSFGIR HSDYLAPLVTDADN*PARRERPHTCLLKVCESA SCPPLSLSLCASWRKAWGGATAGAVSQLRTQRG GARASGEAGTPALRRVRTSRPHRGREGDSREPQT PETHAGRDRPGITPADGPGPRRPGSSACPGAPG DWPGRGVQGFPGAATAELRPPGGPTRLPSWGAA APPPAVDRCARTLNPTVPTRGRGRVPGLGSGASG RGQRPKQVRAGLKSRGGEAGGRHGRGRHRHGTE GRTPERHGKGVGTGARGSDTDAGRPQTPDVSGI PAGPERWQP**PREPGPHPRLVTSPGKGARDFPT LRQSPSSSRS*SA*SETEAA*AKRTSGASWPLW *MGARRAHRSGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARCQ*SADVVWGTQEAPWISWSLF LDSMSSRAQSRCKCCWTRRAAVSWVRVSSWFLR TESYMQPEM*QPPWTACHSSQPPFSVRNSWRGCP LWWWTLSSEGPPSSPTRSRPGSWQRRWLAWEPA *GFGSRQR*PPWTSPWVAAWATPWRWRRRCSAW TAQARQT*GTWSPRSGAPCSGSADTRGLRPRALP GWPRRWTTARPLAASSGCWRRRAWIPVWPEPCA REVPQNAGSCCLAPGSRRSCWRPQMAPWSWSGR CRWRWCCTSSGPGAAALGSRSAWGWAQSCWSTWV RGCAVGPPGSACTGTAPRSAARRAAPCRRRSYS PTARHSPPPRPSQSSFCRRSNKAPLPRNLVSAW AGAEGSRAAEAGAVSMNT*PPAGSAFRARAESLS AAAPVCACAAPGTPAPCL*ACGARFP*AGGAER RELEVGASSRAWSTDARPRREVRTPGLGGEEVA LRTRWTPG*FPSSGYCAGLAGHTPGRAGGRSEAG APVGGSQQAPVFSRPSTVITRSRGASSGPGTFL ATRALALLGRAQTQNRPGGGAVSPREGALSRRG Q*PGVLRPPPSKPSAGARGKGSKVTNAKAGRRL* ARRSCWEAWM*GGWGFVAGGSVVRLCQERLNTA GGQSLSSGGRSCRFSAGSVEEGRGWLKWLEVTG KCWRNRLLSGIASQRVTHLLLRNGFIANR*RG*F RQGMHLECFPSARGSPC*GAGAGQGAVTGW*SQ VEDRSDRCGCPGVDVWWRWSSELR*AAANAC*A *TWAGSFRWVAGLKSQGQPRHCPGPSLLAPDACA CFQEHQIHAAADSEPHSLAQALSAQASGPPWDP GRPGPASEVLAFVKAGPCRDRWAGPAPGPWASN PAADHRPVRGWTRWGLAGPEG*EGEAAAAKANRS PAPGSCGPGRLPPAGSQRPGSLQG*LPGPVGAD VLWLHSLP*HLPRRAGEAGAGGAAAGSRAWFAS SAACLHHCGPRAGRR*SHGPLRPGLPPKTVGSDR LHQTGCPG*SQLPRVLQCRPQG*GPGLHRGPLH CHLPAQP*RPLHGLLRPEQIG*ADLRQCAAAHG GFPQCPVLSHCSLGPIIKRAAF

Frame 2

QHENLLPPPHPQVIPFTKSGSRLGPREFTSLPN APGVRVMALDPLPAPSSTQPGRKLAFVLLAPGI RSRPRPAPR*SQGSGKVRSPRICGAGAAATAFC PQVLRAVSAPHPRYGLGRLGGPLAATPAPRPHRG ALRRPRAQIQAAAQHR*KGQAPGLRGGTAPGGG KGKRPGRPALGAEPCLLEGRGGLCRGRVRVADG AWGWARPDANSDAPRLRPA*PVAAARPRLHLRRA LPHAADDVRPRARPPGARSHDRARHRRRPRLLH LRPPTPLSALPHSGTWSGPPGPWPPQRRTASRE AHLGTPDLNPESPSDTLTPHIRYPHIRISNPKPR TPRGFDKSWLRLPTSPRTPSRVPTRLPRSSSPP HP*SAPQAGTSRSERGTRRTPVRLGTPLLPETG VSRRNSRAQVPRPIRCPRSWVRASSAKSPPQLAP STAAERLAVSSRTSARS*VQGSTSPGPPSSRFW RGLRSPKTTLGSQGPRPTTWISTGSPAAGVSGS GTRTTWPRW*PTRTTDPPGGSGPTRVCLKSASPH RVRLSLSLSARPGARPGVEPRLGPCPNSEPSGA GPERRARPGPQRCAASERRDPTEGGRGTLGSHR RPRPTPGGTGQGSPPPTAPGPDGPEVPRVRGHRG IGRGAACKASRGRRLPSSALQAAPPACRPGAPP PRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQA GEGSALSRYGRGSSREAGKREADTDEGDTDTGPR GGHRRDTGKGSGQEHVAQTPTPGGRRPRTCQAS PQARSDGSLDDPGNRGPTRAW*LLRGREPGTSR PFARAQAAPGADPHEARRRPPERSGHQGLRGRCG EWERAGRTDRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQGANDQRTWSGAHRRHLG*AGVYS WIQCHPEPRADASAAGPGGLLYRGSE*AAGSCG RNPICSQRCDSHRGQPATHHSLHSQ*ETRGGAV RSGGGR*VRRGRRLPQPGAGPGAGKDAGWRGSQP RASGRGSADRHGQAPGSLRGPRPGGGGGAALHG RRRPARLKGPGHHARGRPALAQRTRGDSGPGRC PGGRGAGRRLGPWPLRADAGGAGRGSRSGPSPVL GKSRRTPAAAASRPGAGGAAGARRWHRGAGPGA AAGAGAARARGRAQPRWGAAPPGGGRRAAGRRG SEAAPWDPLAPRAPGRPRAQRPAEPRPAGGARTL RPRAIRRPLALRRARSAAAAIKLLCRETLSVLG RERKDPGLRRRGPSR*TRDPRRAPPSAHALRAC QRLRPCAHAQLRGRLRPACERVAPAFPEPAGQSA GSWRSALPLVLGPLTRGPAARCGRRGWEGRR*P *GLAGLRGSFPAPATARGWRGTPQGALEAGARL GRPWEAPSRHRCSRGQAQL*RARAALRVVLEPFW PRGRWPCWGGHKPRTARAGVQ*VLGRVPSAGGG SDRES*DLHLANLLRGPVGKAQRSPTQRQGVGC EPGGAAGKPGCEEGGVLWRVEVSCVSARRG*TQP AGRV*APGVGRAGFLLGVWRKAAVG*SGWR*QE SVGGIGCSRGLQARELPTSF*EMGLLQIDNVVS SGKACTWNAFRQQEVHLAEVQVQGRVR*QAGDPR *RTGVTGVVAQVWMFGGGGVLSSGEQLQMPVKP ERGLGPSDGWLVSSRRGSPGTVLGLPFWLLTPV LVSRSIRSMLLLTRSPTAWHRLSQLKPRVLPGTL GGQALHLRSWLLSRQGPAETGGQGQPQGPGLRT RLLITGLFGAGLGGAWLALRAEKERLQQQKRTE ALRQAAVGQGDFHLLDHRGRARCKADFRGQWVLM YFGFTHCPDICPDELEKLVQVVRQLEAEPGLPP VQPVFITVDPERDDVEAMARYVQDFHPRLLGLT GSTKQVAQASHSYRVYYNAGPKDEDQDYIVDHSI AIYLLNPDGLFTDYYGRSRSAEQISDSVRRHMA AFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.shed1.aAug05

AGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGC CCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGG AGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGA GCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGC CCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGC GCAGGGCGCACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGC CCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTT CGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGG ACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAG ACGGGAGCCTATACCGCACATGCAGCAACCAGTCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

SGPLCANPEPYGPDPRARPGTWAGIRSKRARAA P*AGTGGAQVARRGSGRQTRTRATQTRDRGADTG ETRERGRDRSTWLRHRRREAADPGRVRHPRRPG AMAALMTPGTGAPPAPGDFSGEGSQGLPDPSPE PKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSA QGAQIGAWGGLGVPDPDWEVSPRDFGSLGVRRC PTTSTGPRVPHRCGLPPSRVPPHTRGHADGHPT SGHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKAS

Frame 1

VDRCARTLNPTVPTRGRGRVPGLGSGASGRGQR PKQVRAGLKSRGGEAGGRHGRGRHRHGTEGRTP ERHGKGVGTGARGSDTDAGRPQTPDVSGIPAGPE RWQP**PREPGPHPRLVTSPGKGARDFPTLRQS PSSSRS*SA*SETEAA*AKRTSGASWPLW*MGA RRAHRSVRGEGWAFLTPTGRSARETLGPWGCDGA PLPAPAPGCPTAVGCHPHAYPHIPGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLV DKHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPP

Frame 2

WTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSA LSRYGRGSSREAGKREADTDEGDTDTGPRGGHR RDTGKGSGQEHVAQTPTPGGRRPRTCQASPQAR SDGSLDDPGNRGPTRAW*LLRGREPGTSRPFARA QAAPGADPHEARRRPPERSGHQGLRGRCGEWER AGRTDRCVGRVGRS*PRLGGQPERLWVPGGATV PHYQHRPQGAPPLWAATLTRTPTYQGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFP DGSLYRTCSNQSIHRQLPTSSLAQSLQ