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Sequences

Sequence ECGF1.fAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCC TGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGG CATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAG CATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGG GGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTG CTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACA GCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACG TTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGC GGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCA GACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGC TGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGA ACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTG CTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTG GGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATT CGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATC CAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTG TGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCG GCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGAC TCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGC TCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGG CCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCT GAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGC TGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGA GCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCG GTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCAC TTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTG ACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTC CTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCAT CAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTG CATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAG GCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGC TGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACT CACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCA TCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGT TGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTC AACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCC TGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRAQAAPGADPHEARRRPP ERSGHQGLRGRCGEWERAGRTDRGHADGHPTSG HGSGGDLGADPGPGSVGTAAGVARGLAPAACGQ AFHRGCG*QGQPGPRTCPGGMWLQGANDQRTWSG AHRRHLG*AGVYSWIQCHPEPRADASAAGPGGL LYRGSE*AAGSCGRNPICSQRCDSHRGQPATHH SSRFSIVSNLLCLPQSLHSQ*ETRGGAVRSGGGR *VRRGRRLPQPGAGPGAGKDAGWRGSQPRASGR GSADRHGQAPGSLRGPRPGGGGGAALHGRRRPA RLKGPGHHARGRPALAQRTRGDSGPGRCPGGRGA GRRLGPWPLRADAGGAGRGSRSGPSPVLGKSRR TPAAAASRPGAGGAAGARRWHRGAGPGAAAGAG AARARGRAQPRWGAAPPGGGRRAAGRRGSEAAPW DPLAPRAPGRPRAQRPAEPRPAGGARTLRPRAI RRPLALRRARSAAAAIKLLCRETLSVLGRERKD PGLRRRGPSR*TRDPRRAPPSAHALRACQRLRPC AHAQLRGRLRPACERVAPAFPEPAGQSAGSWRS ALPLVLGPLTRGPAARCGRRGWEGRR*P*GLAG LRGSFPAPATARGWRGTPQGALEAGARLGRPWEA PSRHRCSRGQAQL*RARAALRVVLEPFWPRGRW PCWGGHKPRTARAGVQ*VLGRVPSAGGGSDRES *DLHLANLLRGPVGKAQRSPTQRQGVGCEPGGAA GKPGCEEGGVLWRVEVSCVSARRG*TQPAGRV* APGVGRAGFLLGVWRKAAVG*SGWR*QESVGGI GCSRGLQARELPTSF*EMGLLQIDNVVSSGKACT WNAFRQQEVHLAEVQVQGRVR*QAGDPR*RTGV TGVVAQVWMFGGGGVLSSGEQLQMPVKPERGLG PSDGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSI RSMLLLTRSPTAWHRLSQLKPRVLPGTLGGQAL HLRSWLLSRQGPAETGGQGQPQGPGLRTRLLIT GLFGAGLGGAWLALRAEKERLQQQKRTEALRQAA VGQGDFHLLDHRGRARCKADFRGQWVLMYFGFT HCPDICPDELEKLVQVVRQLEAEPGLPPVQPVF ITVDPERDDVEAMARYVQDFHPRLLGLTGSTKQV AQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLL NPDGLFTDYYGRSRSAEQISDSVRRHMAAFRSV LS*ATAVWAPSLNGLRL

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AEPKQLPELIRMKRDGGR LSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRG MDLEETSVLTQALAQSGQQLEWPEAWRQQLVDK HSTGGVGDKVSLVLAPALAACGCKVPMISGRGL GHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGC CIVGQSEQLVPADGILYAARDVTATVDSLPLIT APGSPLSPTSSASPKASILSKKLVEGLSALVVD VKFGGAAVFPNQEQARELAKTLVGVGASLGLRVA AALTAMDKPLGRCVGHALEVEEALLCMDGAGPP DLRDLVTTLGGALLWLSGHAGTQAQGAARVAAA LDDGSALGRFERMLAAQGVDPGLARALCSGSPAE RRQLLPRAREQEELLAPADGTVELVRALPLALV LHELGAGRSRAGEPLRLGVGAELLVDVGQRLRR GTPWLRVHRDGPALSGPQSRALQEALVLSDRAPF AAPSPFAELVLPPQQ*SSFAAKPCQCLGGSGRI QGCGGGGRLDEHVTPGGLRLPRTR*EPVSGCAR VRMRSSGDACALPVSVWRPLSLSRRGRAQGAGGR RFLSCLVH*RAAPPRGADAGAGRGGGSPEDSLD SGVVSQLRLLRGAGGAHPRARWRPERGWGARGR LPAGTGVLAAKHSYNALARRFEWSWNLSGHEGAG LAGEGTNPEPPGRGCSESSGGCPQQEGAVTGSP ETST*QTFCGGPWERLKGHQRKGRASAVSPEEL LGSLDVRRVGFCGGWKCRASLPGEVKHSRRAESE LRG*VVQVFCWECGGRPRLVEVAGGNRKVLEES VALGDCKPESYPPPFKKWVYCK*ITWLVQARHA LGMLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE* QVWLPRCGCLVEVEF*AQVSSCKCLLSLNVGWV LQMGGWSQVAGAAQALSWAFPSGS*RLCLFPGA SDPCCC*LGAPQLGTGSLSSSLGSSLGPWEARPC I*GPGFCQGRALQRQVGRASPRALGFEPGC*SQ ACSGLDSVGPGWP*GLRRRGCSSKSEQKPCARQ LWARATSTCWITEAGLAARLTSGASGC*CTLASL TALTSAQTSWRSWCRWCGSWKQSLVCLQCSLSS SLWTPSGTTLKPWPATSRTSTQDCWV*PAPPNR LPRLVTVTACTTMQAPRMRTRTTSWTTPLPSTCS TLTASSRITTAGADRLSRSQTVCGGTWRLSAVS CLEPLQSGPHH*TGCV*

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQSPSSSRS*SA*SETEAA *AKRTSGASWPLW*MGARRAHRSGPC*WPSDFG AWIWRRPRC*PRPWLSRDSSWSGQRPGASSLWTS IPQGVWVTRSAWSSHLPWRHVAARCQ*SADVVW GTQEAPWISWSLFLDSMSSRAQSRCKCCWTRRA AVSWVRVSSWFLRTESYMQPEM*QPPWTACHSSQ LQVLHCLQPPLPPPKPPFSVRNSWRGCPLWWWT LSSEGPPSSPTRSRPGSWQRRWLAWEPA*GFGS RQR*PPWTSPWVAAWATPWRWRRRCSAWTAQARQ T*GTWSPRSGAPCSGSADTRGLRPRALPGWPRR WTTARPLAASSGCWRRRAWIPVWPEPCAREVPQ NAGSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWC CTSSGPGAAALGSRSAWGWAQSCWSTWVRGCAV GPPGSACTGTAPRSAARRAAPCRRRSYSPTARH SPPPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGS RAAEAGAVSMNT*PPAGSAFRARAESLSAAAPV CACAAPGTPAPCL*ACGARFP*AGGAERRELEV GASSRAWSTDARPRREVRTPGLGGEEVALRTRWT PG*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGG SQQAPVFSRPSTVITRSRGASSGPGTFLATRAL ALLGRAQTQNRPGGGAVSPREGALSRRGQ*PGVL RPPPSKPSAGARGKGSKVTNAKAGRRL*ARRSC WEAWM*GGWGFVAGGSVVRLCQERLNTAGGQSL SSGGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNR LLSGIASQRVTHLLLRNGFIANR*RG*FRQGMH LECFPSARGSPC*GAGAGQGAVTGW*SQVEDRS DRCGCPGVDVWWRWSSELR*AAANAC*A*TWAGS FRWVAGLKSQGQPRHCPGPSLLAPDACACFQEH QIHAAADSEPHSLAQALSAQASGPPWDPGRPGP ASEVLAFVKAGPCRDRWAGPAPGPWASNPAADHR PVRGWTRWGLAGPEG*EGEAAAAKANRSPAPGS CGPGRLPPAGSQRPGSLQG*LPGPVGADVLWLH SLP*HLPRRAGEAGAGGAAAGSRAWFASSAACLH HCGPRAGRR*SHGPLRPGLPPKTVGSDRLHQTG CPG*SQLPRVLQCRPQG*GPGLHRGPLHCHLPA QP*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCP VLSHCSLGPIIKRAAF

Sequence ECGF1.aAug05

AACAGCACGAAAATCTCCTGCCCCCACCCCACCCCCAGGTGATTCCCTTCACCAAGTCTGGGTCTAGACTTGGCCCCCGCGAGTTCACGTCCCTGCCTAA TGCGCCTGGAGTCCGGGTGATGGCCCTGGATCCGCTCCCGGCCCCAAGCTCCACACAGCCGGGAAGGAAGCTCGCGTTTGTTTTGCTGGCGCCAGGGATA CGGTCCAGGCCCCGCCCCGCCCCGCGCTGATCCCAGGGTAGTGGGAAAGTGCGCTCCCCAAGAATTTGCGGTGCAGGGGCCGCGGCCACCGCCTTCTGTC CGCAGGTGCTGCGAGCCGTAAGCGCCCCCCACCCGCGCTATGGGCTCGGACGCCTGGGTGGGCCTTTGGCGGCCACACCGGCCCCGCGGCCCCATCGCGG CGCACTACGGAGGCCCCGGGCCCAAATACAAGCTGCCGCCCAACACCGGTAGAAGGGGCAGGCCCCGGGATTGAGGGGTGGGACGGCGCCTGGGGGAGGG AAGGGGAAGAGACCAGGGAGGCCTGCGCTCGGGGCTGAACCGTGCCTGCTAGAGGGGAGAGGGGGTCTGTGCCGGGGGAGGGTCCGTGTTGCAGATGGGG CTTGGGGCTGGGCCCGGCCAGACGCTAACTCGGATGCTCCCAGGCTACGCCCTGCATGACCCGTCGCGGCCGCGCGCCCCCGCCTTCACCTTCGGCGCGC GCTTCCCCACGCAGCAGACGACGTGCGGCCCCGGGCCAGGCCACCTGGTGCCCGCTCGCATGACCGTGCGCGGCACCGACGGCGCCCCCGCCTACTCCAT CTACGGCCGCCCACGCCGCTCAGCGCCCTTCCTCACTCCGGGACCTGGTCAGGACCCCCGGGCCCCTGGCCACCCCAACGCCGAACTGCCTCCAGGGAGG CCCACCTGGGAACCCCCGACCTGAACCCCGAGTCCCCCTCGGATACCCTAACACCGCATATTCGGTACCCCCATATCCGGATCTCAAATCCCAAACCCCG AACCCCACGGGGCTTTGATAAATCGTGGCTCAGACTCCCCACTAGTCCCAGGACCCCATCTCGGGTACCCACCAGGCTCCCACGCAGTTCTAGCCCCCCA CACCCTTGATCCGCCCCGCAGGCAGGTACTTCCCGGAGCGAGCGGGGAACGCGACGTACCCCAGTGCGCCTCGGCACACCATTGCTCCCCGAAACTGGGG TGTCCAGGCGGAACAGCAGAGCCCAGGTCCCGCGGCCTATACGGTGCCCTCGCTCTTGGGTCCGCGCGTCATCGGCAAAGTCTCCGCCCCAACTTGCTCC ATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGGTCGTGAGTCCAGGGGTCTACAAGTCCCGGGCCCCCCAGTTCACGATTCTGG CGCGGACTTCGCTCCCCCAAGACAACACTCGGAAGCCAGGGCCCGCGGCCTACAACGTGGATCAGCACCGGAAGCCCCGCGGCTGGAGTTTCGGGATCCG GCACTCGGACTACCTGGCCCCGCTGGTGACCGACGCGGACAACTGACCCGCCAGGCGGGAGCGGCCCCACACGTGTTTGCTTAAAGTCTGCGAGTCCGCA TCGTGTCCGCCTCTCTCTCTCTCTCTCTGCGCGTCCTGGCGCAAGGCCTGGGGTGGAGCCACGGCTGGGGCCGTGTCCCAACTCCGAACCCAGCGGGGCG GGGCCCGAGCGTCGGGCGAGGCCGGGACCCCAGCGCTGCGCCGCGTCCGAACGTCGAGACCCCACCGAGGGCGGGAGGGGGACTCTCGGGAGCCACAGAC GCCCGAGACCCACGCCGGGCGGGACCGGCCAGGGATCACCCCCGCCGACGGCCCCGGGCCCCGACGGCCCGGAAGTTCCGCGTGTCCGGGGGCACCGGGG GATTGGCCGGGGCGCGGCGTGCAAGGCTTCCCGGGGGCGGCGACTGCCGAGCTCCGCCCTCCAGGCGGCCCCACCCGCCTGCCGTCCTGGGGCGCCGCCG CCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGG GCGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAG GGGCGGACACCGGAGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCC CCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGAC CCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGG TGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCT GGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTC CTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTC CTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGA CGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCC GCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCC TAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGA CGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCC CGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCT CGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCT GCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGT CAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCT CCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGG CGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTC AGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGC AGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCC TGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGG GGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTG GCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGC AGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTG AGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCC GGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAA AGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTT CAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAG GTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCT GAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGC TTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCT GGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACC CGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAG CCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGAT GTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCA GTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCG GCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCAT TGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCG GCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

NSTKISCPHPTPR*FPSPSLGLDLAPASSRPCL MRLESG*WPWIRSRPQAPHSREGSSRLFCWRQGY GPGPAPPRADPRVVGKCAPQEFAVQGPRPPPSV RRCCEP*APPTRAMGSDAWVGLWRPHRPRGPIA AHYGGPGPKYKLPPNTGRRGRPRD*GVGRRLGEG RGRDQGGLRSGLNRAC*RGEGVCAGGGSVLQMG LGAGPGQTLTRMLPGYALHDPSRPRAPAFTFGA RFPTQQTTCGPGPGHLVPARMTVRGTDGAPAYSI YGRPRRSAPFLTPGPGQDPRAPGHPNAELPPGR PTWEPPT*TPSPPRIP*HRIFGTPISGSQIPNP EPHGALINRGSDSPLVPGPHLGYPPGSHAVLAPH TLDPPRRQVLPGASGERDVPQCASAHHCSPKLG CPGGTAEPRSRGLYGALALGSARHRQSLRPNLL HLRPQSGWQFLRGPQQGRESRGLQVPGPPVHDSG ADFAPPRQHSEARARGLQRGSAPEAPRLEFRDP ALGLPGPAGDRRGQLTRQAGAAPHVFA*SLRVR IVSASLSLSLRVLAQGLGWSHGWGRVPTPNPAGR GPSVGRGRDPSAAPRPNVETPPRAGGGLSGATD ARDPRRAGPARDHPRRRPRAPTARKFRVSGGTG GLAGARRARLPGGGDCRAPPSRRPHPPAVLGRRR PAAGSGPLCANPEPYGPDPRARPGTWAGIRSKR ARAAP*AGTGGAQVARRGSGRQTRTRATQTRDR GADTGETRERGRDRSTWLRHRRREAADPGRVRHP RRPGAMAALMTPGTGAPPAPGDFSGEGSQGLPD PSPEPKQLPELIRMKRDGGRLSEADIRGFVAAV VNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQAL AQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLV LAPALAACGCKVPMISGRGLGHTGGTLDKLESI PGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAD GILYAARDVTATVDSLPLITASILSKKLVEGLS ALVVDVKFGGAAVFPNQEQARELAKTLVGVGAS LGLRVAAALTAMDKPLGRCVGHALEVEEALLCMD GAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAA RVAAALDDGSALGRFERMLAAQGVDPGLARALC SGSPAERRQLLPRAREQEELLAPADGTVELVRAL PLALVLHELGAGRSRAGEPLRLGVGAELLVDVG QRLRRGTPWLRVHRDGPALSGPQSRALQEALVL SDRAPFAAPSPFAELVLPPQQ*SSFAAKPCQCLG GSGRIQGCGGGGRLDEHVTPGGLRLPRTR*EPV SGCARVRMRSSGDACALPVSVWRPLSLSRRGRA QGAGGRRFLSCLVH*RAAPPRGADAGAGRGGGSP EDSLDSGVVSQLRLLRGAGGAHPRARWRPERGW GARGRLPAGTGVLAAKHSYNALARRFEWSWNLS GHEGAGLAGEGTNPEPPGRGCSESSGGCPQQEGA VTGSPETST*QTFCGGPWERLKGHQRKGRASAV SPEELLGSLDVRRVGFCGGWKCRASLPGEVKHS RRAESELRG*VVQVFCWECGGRPRLVEVAGGNRK VLEESVALGDCKPESYPPPFKKWVYCK*ITWLV QARHALGMLSVSKRFTLLRCRCRAGCGDRLVIP GRGQE*QVWLPRCGCLVEVEF*AQVSSCKCLLSL NVGWVLQMGGWSQVAGAAQALSWAFPSGS*RLC LFPGASDPCCC*LGAPQLGTGSLSSSLGSSLGP WEARPCI*GPGFCQGRALQRQVGRASPRALGFEP GC*SQACSGLDSVGPGWP*GLRRRGCSSKSEQK PCARQLWARATSTCWITEAGLAARLTSGASGC* CTLASLTALTSAQTSWRSWCRWCGSWKQSLVCLQ CSLSSSLWTPSGTTLKPWPATSRTSTQDCWV*P APPNRLPRLVTVTACTTMQAPRMRTRTTSWTTP LPSTCSTLTASSRITTAGADRLSRSQTVCGGTWR LSAVSCLEPLQSGPHH*TGCV*

Frame 1

TARKSPAPTPPPGDSLHQVWV*TWPPRVHVPA* CAWSPGDGPGSAPGPKLHTAGKEARVCFAGARD TVQAPPRPALIPG*WESALPKNLRCRGRGHRLLS AGAASRKRPPPALWARTPGWAFGGHTGPAAPSR RTTEAPGPNTSCRPTPVEGAGPGIEGWDGAWGR EGEETREACARG*TVPARGERGSVPGEGPCCRWG LGLGPARR*LGCSQATPCMTRRGRAPPPSPSAR ASPRSRRRAAPGQATWCPLA*PCAAPTAPPPTP STAAHAAQRPSSLRDLVRTPGPLATPTPNCLQGG PPGNPRPEPRVPLGYPNTAYSVPPYPDLKSQTP NPTGL**IVAQTPH*SQDPISGTHQAPTQF*PP TPLIRPAGRYFPERAGNATYPSAPRHTIAPRNWG VQAEQQSPGPAAYTVPSLLGPRVIGKVSAPTCS IYGRRAAGSFFEDLSKVVSPGVYKSRAPQFTIL ARTSLPQDNTRKPGPAAYNVDQHRKPRGWSFGIR HSDYLAPLVTDADN*PARRERPHTCLLKVCESA SCPPLSLSLCASWRKAWGGATAGAVSQLRTQRG GARASGEAGTPALRRVRTSRPHRGREGDSREPQT PETHAGRDRPGITPADGPGPRRPGSSACPGAPG DWPGRGVQGFPGAATAELRPPGGPTRLPSWGAA APPPAVDRCARTLNPTVPTRGRGRVPGLGSGASG RGQRPKQVRAGLKSRGGEAGGRHGRGRHRHGTE GRTPERHGKGVGTGARGSDTDAGRPQTPDVSGI PAGPERWQP**PREPGPHPRLVTSPGKGARDFPT LRQSPSSSRS*SA*SETEAA*AKRTSGASWPLW *MGARRAHRSGPC*WPSDFGAWIWRRPRC*PRP WLSRDSSWSGQRPGASSLWTSIPQGVWVTRSAWS SHLPWRHVAARCQ*SADVVWGTQEAPWISWSLF LDSMSSRAQSRCKCCWTRRAAVSWVRVSSWFLR TESYMQPEM*QPPWTACHSSQPPFSVRNSWRGCP LWWWTLSSEGPPSSPTRSRPGSWQRRWLAWEPA *GFGSRQR*PPWTSPWVAAWATPWRWRRRCSAW TAQARQT*GTWSPRSGAPCSGSADTRGLRPRALP GWPRRWTTARPLAASSGCWRRRAWIPVWPEPCA REVPQNAGSCCLAPGSRRSCWRPQMAPWSWSGR CRWRWCCTSSGPGAAALGSRSAWGWAQSCWSTWV RGCAVGPPGSACTGTAPRSAARRAAPCRRRSYS PTARHSPPPRPSQSSFCRRSNKAPLPRNLVSAW AGAEGSRAAEAGAVSMNT*PPAGSAFRARAESLS AAAPVCACAAPGTPAPCL*ACGARFP*AGGAER RELEVGASSRAWSTDARPRREVRTPGLGGEEVA LRTRWTPG*FPSSGYCAGLAGHTPGRAGGRSEAG APVGGSQQAPVFSRPSTVITRSRGASSGPGTFL ATRALALLGRAQTQNRPGGGAVSPREGALSRRG Q*PGVLRPPPSKPSAGARGKGSKVTNAKAGRRL* ARRSCWEAWM*GGWGFVAGGSVVRLCQERLNTA GGQSLSSGGRSCRFSAGSVEEGRGWLKWLEVTG KCWRNRLLSGIASQRVTHLLLRNGFIANR*RG*F RQGMHLECFPSARGSPC*GAGAGQGAVTGW*SQ VEDRSDRCGCPGVDVWWRWSSELR*AAANAC*A *TWAGSFRWVAGLKSQGQPRHCPGPSLLAPDACA CFQEHQIHAAADSEPHSLAQALSAQASGPPWDP GRPGPASEVLAFVKAGPCRDRWAGPAPGPWASN PAADHRPVRGWTRWGLAGPEG*EGEAAAAKANRS PAPGSCGPGRLPPAGSQRPGSLQG*LPGPVGAD VLWLHSLP*HLPRRAGEAGAGGAAAGSRAWFAS SAACLHHCGPRAGRR*SHGPLRPGLPPKTVGSDR LHQTGCPG*SQLPRVLQCRPQG*GPGLHRGPLH CHLPAQP*RPLHGLLRPEQIG*ADLRQCAAAHG GFPQCPVLSHCSLGPIIKRAAF

Frame 2

QHENLLPPPHPQVIPFTKSGSRLGPREFTSLPN APGVRVMALDPLPAPSSTQPGRKLAFVLLAPGI RSRPRPAPR*SQGSGKVRSPRICGAGAAATAFC PQVLRAVSAPHPRYGLGRLGGPLAATPAPRPHRG ALRRPRAQIQAAAQHR*KGQAPGLRGGTAPGGG KGKRPGRPALGAEPCLLEGRGGLCRGRVRVADG AWGWARPDANSDAPRLRPA*PVAAARPRLHLRRA LPHAADDVRPRARPPGARSHDRARHRRRPRLLH LRPPTPLSALPHSGTWSGPPGPWPPQRRTASRE AHLGTPDLNPESPSDTLTPHIRYPHIRISNPKPR TPRGFDKSWLRLPTSPRTPSRVPTRLPRSSSPP HP*SAPQAGTSRSERGTRRTPVRLGTPLLPETG VSRRNSRAQVPRPIRCPRSWVRASSAKSPPQLAP STAAERLAVSSRTSARS*VQGSTSPGPPSSRFW RGLRSPKTTLGSQGPRPTTWISTGSPAAGVSGS GTRTTWPRW*PTRTTDPPGGSGPTRVCLKSASPH RVRLSLSLSARPGARPGVEPRLGPCPNSEPSGA GPERRARPGPQRCAASERRDPTEGGRGTLGSHR RPRPTPGGTGQGSPPPTAPGPDGPEVPRVRGHRG IGRGAACKASRGRRLPSSALQAAPPACRPGAPP PRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQA GEGSALSRYGRGSSREAGKREADTDEGDTDTGPR GGHRRDTGKGSGQEHVAQTPTPGGRRPRTCQAS PQARSDGSLDDPGNRGPTRAW*LLRGREPGTSR PFARAQAAPGADPHEARRRPPERSGHQGLRGRCG EWERAGRTDRGHADGHPTSGHGSGGDLGADPGP GSVGTAAGVARGLAPAACGQAFHRGCG*QGQPG PRTCPGGMWLQGANDQRTWSGAHRRHLG*AGVYS WIQCHPEPRADASAAGPGGLLYRGSE*AAGSCG RNPICSQRCDSHRGQPATHHSLHSQ*ETRGGAV RSGGGR*VRRGRRLPQPGAGPGAGKDAGWRGSQP RASGRGSADRHGQAPGSLRGPRPGGGGGAALHG RRRPARLKGPGHHARGRPALAQRTRGDSGPGRC PGGRGAGRRLGPWPLRADAGGAGRGSRSGPSPVL GKSRRTPAAAASRPGAGGAAGARRWHRGAGPGA AAGAGAARARGRAQPRWGAAPPGGGRRAAGRRG SEAAPWDPLAPRAPGRPRAQRPAEPRPAGGARTL RPRAIRRPLALRRARSAAAAIKLLCRETLSVLG RERKDPGLRRRGPSR*TRDPRRAPPSAHALRAC QRLRPCAHAQLRGRLRPACERVAPAFPEPAGQSA GSWRSALPLVLGPLTRGPAARCGRRGWEGRR*P *GLAGLRGSFPAPATARGWRGTPQGALEAGARL GRPWEAPSRHRCSRGQAQL*RARAALRVVLEPFW PRGRWPCWGGHKPRTARAGVQ*VLGRVPSAGGG SDRES*DLHLANLLRGPVGKAQRSPTQRQGVGC EPGGAAGKPGCEEGGVLWRVEVSCVSARRG*TQP AGRV*APGVGRAGFLLGVWRKAAVG*SGWR*QE SVGGIGCSRGLQARELPTSF*EMGLLQIDNVVS SGKACTWNAFRQQEVHLAEVQVQGRVR*QAGDPR *RTGVTGVVAQVWMFGGGGVLSSGEQLQMPVKP ERGLGPSDGWLVSSRRGSPGTVLGLPFWLLTPV LVSRSIRSMLLLTRSPTAWHRLSQLKPRVLPGTL GGQALHLRSWLLSRQGPAETGGQGQPQGPGLRT RLLITGLFGAGLGGAWLALRAEKERLQQQKRTE ALRQAAVGQGDFHLLDHRGRARCKADFRGQWVLM YFGFTHCPDICPDELEKLVQVVRQLEAEPGLPP VQPVFITVDPERDDVEAMARYVQDFHPRLLGLT GSTKQVAQASHSYRVYYNAGPKDEDQDYIVDHSI AIYLLNPDGLFTDYYGRSRSAEQISDSVRRHMA AFRSVLS*ATAVWAPSLNGLRL

Sequence ECGF1.shed1.aAug05

AGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGC CCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGAGGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGG AGAGACACGGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGA GCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGC CCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGC GCAGGGCGCACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGC CCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTT CGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGG ACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAG ACGGGAGCCTATACCGCACATGCAGCAACCAGTCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Intron retention

Frame 0

SGPLCANPEPYGPDPRARPGTWAGIRSKRARAA P*AGTGGAQVARRGSGRQTRTRATQTRDRGADTG ETRERGRDRSTWLRHRRREAADPGRVRHPRRPG AMAALMTPGTGAPPAPGDFSGEGSQGLPDPSPE PKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSA QGAQIGAWGGLGVPDPDWEVSPRDFGSLGVRRC PTTSTGPRVPHRCGLPPSRVPPHTRGHADGHPT SGHGSGGDLGADPGPGSVGTAAGVARGLAPAACG QAFHRGCG*QGQPGPRTCPGGMWLQG*KPPPFQ TGAYTAHAATSPSTGSSQPQAWPKAS

Frame 1

VDRCARTLNPTVPTRGRGRVPGLGSGASGRGQR PKQVRAGLKSRGGEAGGRHGRGRHRHGTEGRTP ERHGKGVGTGARGSDTDAGRPQTPDVSGIPAGPE RWQP**PREPGPHPRLVTSPGKGARDFPTLRQS PSSSRS*SA*SETEAA*AKRTSGASWPLW*MGA RRAHRSVRGEGWAFLTPTGRSARETLGPWGCDGA PLPAPAPGCPTAVGCHPHAYPHIPGAMLMAIRL RGMDLEETSVLTQALAQSGQQLEWPEAWRQQLV DKHSTGGVGDKVSLVLAPALAACGCKVRNHLLSR REPIPHMQQPVHPQAAPNLKPGPKPP

Frame 2

WTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSA LSRYGRGSSREAGKREADTDEGDTDTGPRGGHR RDTGKGSGQEHVAQTPTPGGRRPRTCQASPQAR SDGSLDDPGNRGPTRAW*LLRGREPGTSRPFARA QAAPGADPHEARRRPPERSGHQGLRGRCGEWER AGRTDRCVGRVGRS*PRLGGQPERLWVPGGATV PHYQHRPQGAPPLWAATLTRTPTYQGPC*WPSDF GAWIWRRPRC*PRPWLSRDSSWSGQRPGASSLW TSIPQGVWVTRSAWSSHLPWRHVAARLETTSFP DGSLYRTCSNQSIHRQLPTSSLAQSLQ