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Sequence ECGF1.fAug05
CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCC
TGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGG
CATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAG
CATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGG
GGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTG
CTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACA
GCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACG
TTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGC
GGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCA
GACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGC
TGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGA
ACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTG
CTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTG
GGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATT
CGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATC
CAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTG
TGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCG
GCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGAC
TCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGC
TCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGG
CCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCT
GAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGC
TGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGA
GCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCG
GTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCAC
TTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTG
ACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTC
CTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCAT
CAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTG
CATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAG
GCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGC
TGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACT
CACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCA
TCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGT
TGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTC
AACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCC
TGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA
- ECGF1.F3 (forward primer)
- ECGF1.R30 (reverse primer)
- Alternative acceptor
Frame 0
PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSALSRAQAAPGADPHEARRRPP
ERSGHQGLRGRCGEWERAGRTDRGHADGHPTSG
HGSGGDLGADPGPGSVGTAAGVARGLAPAACGQ
AFHRGCG*QGQPGPRTCPGGMWLQGANDQRTWSG
AHRRHLG*AGVYSWIQCHPEPRADASAAGPGGL
LYRGSE*AAGSCGRNPICSQRCDSHRGQPATHH
SSRFSIVSNLLCLPQSLHSQ*ETRGGAVRSGGGR
*VRRGRRLPQPGAGPGAGKDAGWRGSQPRASGR
GSADRHGQAPGSLRGPRPGGGGGAALHGRRRPA
RLKGPGHHARGRPALAQRTRGDSGPGRCPGGRGA
GRRLGPWPLRADAGGAGRGSRSGPSPVLGKSRR
TPAAAASRPGAGGAAGARRWHRGAGPGAAAGAG
AARARGRAQPRWGAAPPGGGRRAAGRRGSEAAPW
DPLAPRAPGRPRAQRPAEPRPAGGARTLRPRAI
RRPLALRRARSAAAAIKLLCRETLSVLGRERKD
PGLRRRGPSR*TRDPRRAPPSAHALRACQRLRPC
AHAQLRGRLRPACERVAPAFPEPAGQSAGSWRS
ALPLVLGPLTRGPAARCGRRGWEGRR*P*GLAG
LRGSFPAPATARGWRGTPQGALEAGARLGRPWEA
PSRHRCSRGQAQL*RARAALRVVLEPFWPRGRW
PCWGGHKPRTARAGVQ*VLGRVPSAGGGSDRES
*DLHLANLLRGPVGKAQRSPTQRQGVGCEPGGAA
GKPGCEEGGVLWRVEVSCVSARRG*TQPAGRV*
APGVGRAGFLLGVWRKAAVG*SGWR*QESVGGI
GCSRGLQARELPTSF*EMGLLQIDNVVSSGKACT
WNAFRQQEVHLAEVQVQGRVR*QAGDPR*RTGV
TGVVAQVWMFGGGGVLSSGEQLQMPVKPERGLG
PSDGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSI
RSMLLLTRSPTAWHRLSQLKPRVLPGTLGGQAL
HLRSWLLSRQGPAETGGQGQPQGPGLRTRLLIT
GLFGAGLGGAWLALRAEKERLQQQKRTEALRQAA
VGQGDFHLLDHRGRARCKADFRGQWVLMYFGFT
HCPDICPDELEKLVQVVRQLEAEPGLPPVQPVF
ITVDPERDDVEAMARYVQDFHPRLLGLTGSTKQV
AQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLL
NPDGLFTDYYGRSRSAEQISDSVRRHMAAFRSV
LS*ATAVWAPSLNGLRL
Frame 1
PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPGTWAGIRSKRARAAP*AEPKQLPELIRMKRDGGR
LSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRG
MDLEETSVLTQALAQSGQQLEWPEAWRQQLVDK
HSTGGVGDKVSLVLAPALAACGCKVPMISGRGL
GHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGC
CIVGQSEQLVPADGILYAARDVTATVDSLPLIT
APGSPLSPTSSASPKASILSKKLVEGLSALVVD
VKFGGAAVFPNQEQARELAKTLVGVGASLGLRVA
AALTAMDKPLGRCVGHALEVEEALLCMDGAGPP
DLRDLVTTLGGALLWLSGHAGTQAQGAARVAAA
LDDGSALGRFERMLAAQGVDPGLARALCSGSPAE
RRQLLPRAREQEELLAPADGTVELVRALPLALV
LHELGAGRSRAGEPLRLGVGAELLVDVGQRLRR
GTPWLRVHRDGPALSGPQSRALQEALVLSDRAPF
AAPSPFAELVLPPQQ*SSFAAKPCQCLGGSGRI
QGCGGGGRLDEHVTPGGLRLPRTR*EPVSGCAR
VRMRSSGDACALPVSVWRPLSLSRRGRAQGAGGR
RFLSCLVH*RAAPPRGADAGAGRGGGSPEDSLD
SGVVSQLRLLRGAGGAHPRARWRPERGWGARGR
LPAGTGVLAAKHSYNALARRFEWSWNLSGHEGAG
LAGEGTNPEPPGRGCSESSGGCPQQEGAVTGSP
ETST*QTFCGGPWERLKGHQRKGRASAVSPEEL
LGSLDVRRVGFCGGWKCRASLPGEVKHSRRAESE
LRG*VVQVFCWECGGRPRLVEVAGGNRKVLEES
VALGDCKPESYPPPFKKWVYCK*ITWLVQARHA
LGMLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE*
QVWLPRCGCLVEVEF*AQVSSCKCLLSLNVGWV
LQMGGWSQVAGAAQALSWAFPSGS*RLCLFPGA
SDPCCC*LGAPQLGTGSLSSSLGSSLGPWEARPC
I*GPGFCQGRALQRQVGRASPRALGFEPGC*SQ
ACSGLDSVGPGWP*GLRRRGCSSKSEQKPCARQ
LWARATSTCWITEAGLAARLTSGASGC*CTLASL
TALTSAQTSWRSWCRWCGSWKQSLVCLQCSLSS
SLWTPSGTTLKPWPATSRTSTQDCWV*PAPPNR
LPRLVTVTACTTMQAPRMRTRTTSWTTPLPSTCS
TLTASSRITTAGADRLSRSQTVCGGTWRLSAVS
CLEPLQSGPHH*TGCV*
Frame 2
RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRVPGLGSGASGRGQRPKQSPSSSRS*SA*SETEAA
*AKRTSGASWPLW*MGARRAHRSGPC*WPSDFG
AWIWRRPRC*PRPWLSRDSSWSGQRPGASSLWTS
IPQGVWVTRSAWSSHLPWRHVAARCQ*SADVVW
GTQEAPWISWSLFLDSMSSRAQSRCKCCWTRRA
AVSWVRVSSWFLRTESYMQPEM*QPPWTACHSSQ
LQVLHCLQPPLPPPKPPFSVRNSWRGCPLWWWT
LSSEGPPSSPTRSRPGSWQRRWLAWEPA*GFGS
RQR*PPWTSPWVAAWATPWRWRRRCSAWTAQARQ
T*GTWSPRSGAPCSGSADTRGLRPRALPGWPRR
WTTARPLAASSGCWRRRAWIPVWPEPCAREVPQ
NAGSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWC
CTSSGPGAAALGSRSAWGWAQSCWSTWVRGCAV
GPPGSACTGTAPRSAARRAAPCRRRSYSPTARH
SPPPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGS
RAAEAGAVSMNT*PPAGSAFRARAESLSAAAPV
CACAAPGTPAPCL*ACGARFP*AGGAERRELEV
GASSRAWSTDARPRREVRTPGLGGEEVALRTRWT
PG*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGG
SQQAPVFSRPSTVITRSRGASSGPGTFLATRAL
ALLGRAQTQNRPGGGAVSPREGALSRRGQ*PGVL
RPPPSKPSAGARGKGSKVTNAKAGRRL*ARRSC
WEAWM*GGWGFVAGGSVVRLCQERLNTAGGQSL
SSGGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNR
LLSGIASQRVTHLLLRNGFIANR*RG*FRQGMH
LECFPSARGSPC*GAGAGQGAVTGW*SQVEDRS
DRCGCPGVDVWWRWSSELR*AAANAC*A*TWAGS
FRWVAGLKSQGQPRHCPGPSLLAPDACACFQEH
QIHAAADSEPHSLAQALSAQASGPPWDPGRPGP
ASEVLAFVKAGPCRDRWAGPAPGPWASNPAADHR
PVRGWTRWGLAGPEG*EGEAAAAKANRSPAPGS
CGPGRLPPAGSQRPGSLQG*LPGPVGADVLWLH
SLP*HLPRRAGEAGAGGAAAGSRAWFASSAACLH
HCGPRAGRR*SHGPLRPGLPPKTVGSDRLHQTG
CPG*SQLPRVLQCRPQG*GPGLHRGPLHCHLPA
QP*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCP
VLSHCSLGPIIKRAAF
Sequence ECGF1.bAug05
CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGAGCGATGGCA
GCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGC
TCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGC
ACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTG
GAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCAT
GTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAG
CCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGA
GATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGT
TCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGC
GCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTA
AGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACG
ACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCG
GCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCAC
GAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCC
CCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGC
CCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGC
TGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCA
TGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTT
CCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGG
GTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAG
CAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGC
TGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACC
TCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAA
GCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCG
GGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCT
CTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAA
TGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGT
GTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAG
ATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATC
CATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTG
AGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGT
TCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGG
CCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGC
CCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTG
TGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCA
GGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCT
GACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTT
GAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA
- ECGF1.F3 (forward primer)
- ECGF1.R30 (reverse primer)
- Alternative acceptor
Frame 0
PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAGYLGWDPEQAGEGSALSRPQTPDVSGIPAGPERWQ
P**PREPGPHPRLVTSPGKGARDFPTLRQSPSS
SRS*SA*SETEAA*AKRTSGASWPLW*MGARRA
HRSGPC*WPSDFGAWIWRRPRC*PRPWLSRDSSW
SGQRPGASSLWTSIPQGVWVTRSAWSSHLPWRH
VAARCQ*SADVVWGTQEAPWISWSLFLDSMSSR
AQSRCKCCWTRRAAVSWVRVSSWFLRTESYMQPE
M*QPPWTACHSSQPPFSVRNSWRGCPLWWWTLS
SEGPPSSPTRSRPGSWQRRWLAWEPA*GFGSRQ
R*PPWTSPWVAAWATPWRWRRRCSAWTAQARQT*
GTWSPRSGAPCSGSADTRGLRPRALPGWPRRWT
TARPLAASSGCWRRRAWIPVWPEPCAREVPQNA
GSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWCCT
SSGPGAAALGSRSAWGWAQSCWSTWVRGCAVGP
PGSACTGTAPRSAARRAAPCRRRSYSPTARHSP
PPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGSRA
AEAGAVSMNT*PPAGSAFRARAESLSAAAPVCA
CAAPGTPAPCL*ACGARFP*AGGAERRELEVGA
SSRAWSTDARPRREVRTPGLGGEEVALRTRWTPG
*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGGSQ
QAPVFSRPSTVITRSRGASSGPGTFLATRALAL
LGRAQTQNRPGGGAVSPREGALSRRGQ*PGVLRP
PPSKPSAGARGKGSKVTNAKAGRRL*ARRSCWE
AWM*GGWGFVAGGSVVRLCQERLNTAGGQSLSS
GGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNRLL
SGIASQRVTHLLLRNGFIANR*RG*FRQGMHLE
CFPSARGSPC*GAGAGQGAVTGW*SQVEDRSDR
CGCPGVDVWWRWSSELR*AAANAC*A*TWAGSFR
WVAGLKSQGQPRHCPGPSLLAPDACACFQEHQI
HAAADSEPHSLAQALSAQASGPPWDPGRPGPAS
EVLAFVKAGPCRDRWAGPAPGPWASNPAADHRPV
RGWTRWGLAGPEG*EGEAAAAKANRSPAPGSCG
PGRLPPAGSQRPGSLQG*LPGPVGADVLWLHSL
P*HLPRRAGEAGAGGAAAGSRAWFASSAACLHHC
GPRAGRR*SHGPLRPGLPPKTVGSDRLHQTGCP
G*SQLPRVLQCRPQG*GPGLHRGPLHCHLPAQP
*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCPVL
SHCSLGPIIKRAAF
Frame 1
PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPGTWAGIRSKRARAAP*AGRRPRTCQASPQARSDG
SLDDPGNRGPTRAW*LLRGREPGTSRPFARAQAA
PGADPHEARRRPPERSGHQGLRGRCGEWERAGR
TDRGHADGHPTSGHGSGGDLGADPGPGSVGTAA
GVARGLAPAACGQAFHRGCG*QGQPGPRTCPGGM
WLQGANDQRTWSGAHRRHLG*AGVYSWIQCHPE
PRADASAAGPGGLLYRGSE*AAGSCGRNPICSQ
RCDSHRGQPATHHSLHSQ*ETRGGAVRSGGGR*V
RRGRRLPQPGAGPGAGKDAGWRGSQPRASGRGS
ADRHGQAPGSLRGPRPGGGGGAALHGRRRPARL
KGPGHHARGRPALAQRTRGDSGPGRCPGGRGAGR
RLGPWPLRADAGGAGRGSRSGPSPVLGKSRRTP
AAAASRPGAGGAAGARRWHRGAGPGAAAGAGAA
RARGRAQPRWGAAPPGGGRRAAGRRGSEAAPWDP
LAPRAPGRPRAQRPAEPRPAGGARTLRPRAIRR
PLALRRARSAAAAIKLLCRETLSVLGRERKDPG
LRRRGPSR*TRDPRRAPPSAHALRACQRLRPCAH
AQLRGRLRPACERVAPAFPEPAGQSAGSWRSAL
PLVLGPLTRGPAARCGRRGWEGRR*P*GLAGLR
GSFPAPATARGWRGTPQGALEAGARLGRPWEAPS
RHRCSRGQAQL*RARAALRVVLEPFWPRGRWPC
WGGHKPRTARAGVQ*VLGRVPSAGGGSDRES*D
LHLANLLRGPVGKAQRSPTQRQGVGCEPGGAAGK
PGCEEGGVLWRVEVSCVSARRG*TQPAGRV*AP
GVGRAGFLLGVWRKAAVG*SGWR*QESVGGIGC
SRGLQARELPTSF*EMGLLQIDNVVSSGKACTWN
AFRQQEVHLAEVQVQGRVR*QAGDPR*RTGVTG
VVAQVWMFGGGGVLSSGEQLQMPVKPERGLGPS
DGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSIRS
MLLLTRSPTAWHRLSQLKPRVLPGTLGGQALHL
RSWLLSRQGPAETGGQGQPQGPGLRTRLLITGL
FGAGLGGAWLALRAEKERLQQQKRTEALRQAAVG
QGDFHLLDHRGRARCKADFRGQWVLMYFGFTHC
PDICPDELEKLVQVVRQLEAEPGLPPVQPVFIT
VDPERDDVEAMARYVQDFHPRLLGLTGSTKQVAQ
ASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLNP
DGLFTDYYGRSRSAEQISDSVRRHMAAFRSVLS
*ATAVWAPSLNGLRL
Frame 2
RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRVPGLGSGASGRGQRPKQAADPGRVRHPRRPGAMA
ALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQ
LPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGA
QIGAMLMAIRLRGMDLEETSVLTQALAQSGQQL
EWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAA
CGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQS
PEQMQVLLDQAGCCIVGQSEQLVPADGILYAAR
DVTATVDSLPLITASILSKKLVEGLSALVVDVK
FGGAAVFPNQEQARELAKTLVGVGASLGLRVAAA
LTAMDKPLGRCVGHALEVEEALLCMDGAGPPDL
RDLVTTLGGALLWLSGHAGTQAQGAARVAAALD
DGSALGRFERMLAAQGVDPGLARALCSGSPAERR
QLLPRAREQEELLAPADGTVELVRALPLALVLH
ELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGT
PWLRVHRDGPALSGPQSRALQEALVLSDRAPFAA
PSPFAELVLPPQQ*SSFAAKPCQCLGGSGRIQG
CGGGGRLDEHVTPGGLRLPRTR*EPVSGCARVR
MRSSGDACALPVSVWRPLSLSRRGRAQGAGGRRF
LSCLVH*RAAPPRGADAGAGRGGGSPEDSLDSG
VVSQLRLLRGAGGAHPRARWRPERGWGARGRLP
AGTGVLAAKHSYNALARRFEWSWNLSGHEGAGLA
GEGTNPEPPGRGCSESSGGCPQQEGAVTGSPET
ST*QTFCGGPWERLKGHQRKGRASAVSPEELLG
SLDVRRVGFCGGWKCRASLPGEVKHSRRAESELR
G*VVQVFCWECGGRPRLVEVAGGNRKVLEESVA
LGDCKPESYPPPFKKWVYCK*ITWLVQARHALG
MLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE*QV
WLPRCGCLVEVEF*AQVSSCKCLLSLNVGWVLQ
MGGWSQVAGAAQALSWAFPSGS*RLCLFPGASD
PCCC*LGAPQLGTGSLSSSLGSSLGPWEARPCI*
GPGFCQGRALQRQVGRASPRALGFEPGC*SQAC
SGLDSVGPGWP*GLRRRGCSSKSEQKPCARQLW
ARATSTCWITEAGLAARLTSGASGC*CTLASLTA
LTSAQTSWRSWCRWCGSWKQSLVCLQCSLSSSL
WTPSGTTLKPWPATSRTSTQDCWV*PAPPNRLP
RLVTVTACTTMQAPRMRTRTTSWTTPLPSTCSTL
TASSRITTAGADRLSRSQTVCGGTWRLSAVSCL
EPLQSGPHH*TGCV*
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