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Sequences

Sequence ECGF1.fAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCC TGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGG CATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAG CATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGG GGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTG CTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACA GCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACG TTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGC GGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCA GACTTAAGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGC TGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGA ACGCCGGCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTG CTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTG GGACCCCCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATT CGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATC CAGGGCTGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTG TGCGCATGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCG GCGCTTCCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGAC TCCGGGGTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGC TCCCAGCAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGG CCTTGCTGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCT GAGACCTCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGC TGGGAAGCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGA GCTCCGGGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCG GTTGCTCTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCAC TTGGAATGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTG ACAGGTGTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTC CTTCAGATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCAT CAGATCCATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTG CATCTGAGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAG GCCTGTTCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGC TGTGGGCCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACT CACTGCCCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCA TCACTGTGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGT TGCCCAGGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTC AACCCTGACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCC TGTCTTGAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Alternative acceptor

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRAQAAPGADPHEARRRPP ERSGHQGLRGRCGEWERAGRTDRGHADGHPTSG HGSGGDLGADPGPGSVGTAAGVARGLAPAACGQ AFHRGCG*QGQPGPRTCPGGMWLQGANDQRTWSG AHRRHLG*AGVYSWIQCHPEPRADASAAGPGGL LYRGSE*AAGSCGRNPICSQRCDSHRGQPATHH SSRFSIVSNLLCLPQSLHSQ*ETRGGAVRSGGGR *VRRGRRLPQPGAGPGAGKDAGWRGSQPRASGR GSADRHGQAPGSLRGPRPGGGGGAALHGRRRPA RLKGPGHHARGRPALAQRTRGDSGPGRCPGGRGA GRRLGPWPLRADAGGAGRGSRSGPSPVLGKSRR TPAAAASRPGAGGAAGARRWHRGAGPGAAAGAG AARARGRAQPRWGAAPPGGGRRAAGRRGSEAAPW DPLAPRAPGRPRAQRPAEPRPAGGARTLRPRAI RRPLALRRARSAAAAIKLLCRETLSVLGRERKD PGLRRRGPSR*TRDPRRAPPSAHALRACQRLRPC AHAQLRGRLRPACERVAPAFPEPAGQSAGSWRS ALPLVLGPLTRGPAARCGRRGWEGRR*P*GLAG LRGSFPAPATARGWRGTPQGALEAGARLGRPWEA PSRHRCSRGQAQL*RARAALRVVLEPFWPRGRW PCWGGHKPRTARAGVQ*VLGRVPSAGGGSDRES *DLHLANLLRGPVGKAQRSPTQRQGVGCEPGGAA GKPGCEEGGVLWRVEVSCVSARRG*TQPAGRV* APGVGRAGFLLGVWRKAAVG*SGWR*QESVGGI GCSRGLQARELPTSF*EMGLLQIDNVVSSGKACT WNAFRQQEVHLAEVQVQGRVR*QAGDPR*RTGV TGVVAQVWMFGGGGVLSSGEQLQMPVKPERGLG PSDGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSI RSMLLLTRSPTAWHRLSQLKPRVLPGTLGGQAL HLRSWLLSRQGPAETGGQGQPQGPGLRTRLLIT GLFGAGLGGAWLALRAEKERLQQQKRTEALRQAA VGQGDFHLLDHRGRARCKADFRGQWVLMYFGFT HCPDICPDELEKLVQVVRQLEAEPGLPPVQPVF ITVDPERDDVEAMARYVQDFHPRLLGLTGSTKQV AQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLL NPDGLFTDYYGRSRSAEQISDSVRRHMAAFRSV LS*ATAVWAPSLNGLRL

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AEPKQLPELIRMKRDGGR LSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRG MDLEETSVLTQALAQSGQQLEWPEAWRQQLVDK HSTGGVGDKVSLVLAPALAACGCKVPMISGRGL GHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGC CIVGQSEQLVPADGILYAARDVTATVDSLPLIT APGSPLSPTSSASPKASILSKKLVEGLSALVVD VKFGGAAVFPNQEQARELAKTLVGVGASLGLRVA AALTAMDKPLGRCVGHALEVEEALLCMDGAGPP DLRDLVTTLGGALLWLSGHAGTQAQGAARVAAA LDDGSALGRFERMLAAQGVDPGLARALCSGSPAE RRQLLPRAREQEELLAPADGTVELVRALPLALV LHELGAGRSRAGEPLRLGVGAELLVDVGQRLRR GTPWLRVHRDGPALSGPQSRALQEALVLSDRAPF AAPSPFAELVLPPQQ*SSFAAKPCQCLGGSGRI QGCGGGGRLDEHVTPGGLRLPRTR*EPVSGCAR VRMRSSGDACALPVSVWRPLSLSRRGRAQGAGGR RFLSCLVH*RAAPPRGADAGAGRGGGSPEDSLD SGVVSQLRLLRGAGGAHPRARWRPERGWGARGR LPAGTGVLAAKHSYNALARRFEWSWNLSGHEGAG LAGEGTNPEPPGRGCSESSGGCPQQEGAVTGSP ETST*QTFCGGPWERLKGHQRKGRASAVSPEEL LGSLDVRRVGFCGGWKCRASLPGEVKHSRRAESE LRG*VVQVFCWECGGRPRLVEVAGGNRKVLEES VALGDCKPESYPPPFKKWVYCK*ITWLVQARHA LGMLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE* QVWLPRCGCLVEVEF*AQVSSCKCLLSLNVGWV LQMGGWSQVAGAAQALSWAFPSGS*RLCLFPGA SDPCCC*LGAPQLGTGSLSSSLGSSLGPWEARPC I*GPGFCQGRALQRQVGRASPRALGFEPGC*SQ ACSGLDSVGPGWP*GLRRRGCSSKSEQKPCARQ LWARATSTCWITEAGLAARLTSGASGC*CTLASL TALTSAQTSWRSWCRWCGSWKQSLVCLQCSLSS SLWTPSGTTLKPWPATSRTSTQDCWV*PAPPNR LPRLVTVTACTTMQAPRMRTRTTSWTTPLPSTCS TLTASSRITTAGADRLSRSQTVCGGTWRLSAVS CLEPLQSGPHH*TGCV*

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQSPSSSRS*SA*SETEAA *AKRTSGASWPLW*MGARRAHRSGPC*WPSDFG AWIWRRPRC*PRPWLSRDSSWSGQRPGASSLWTS IPQGVWVTRSAWSSHLPWRHVAARCQ*SADVVW GTQEAPWISWSLFLDSMSSRAQSRCKCCWTRRA AVSWVRVSSWFLRTESYMQPEM*QPPWTACHSSQ LQVLHCLQPPLPPPKPPFSVRNSWRGCPLWWWT LSSEGPPSSPTRSRPGSWQRRWLAWEPA*GFGS RQR*PPWTSPWVAAWATPWRWRRRCSAWTAQARQ T*GTWSPRSGAPCSGSADTRGLRPRALPGWPRR WTTARPLAASSGCWRRRAWIPVWPEPCAREVPQ NAGSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWC CTSSGPGAAALGSRSAWGWAQSCWSTWVRGCAV GPPGSACTGTAPRSAARRAAPCRRRSYSPTARH SPPPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGS RAAEAGAVSMNT*PPAGSAFRARAESLSAAAPV CACAAPGTPAPCL*ACGARFP*AGGAERRELEV GASSRAWSTDARPRREVRTPGLGGEEVALRTRWT PG*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGG SQQAPVFSRPSTVITRSRGASSGPGTFLATRAL ALLGRAQTQNRPGGGAVSPREGALSRRGQ*PGVL RPPPSKPSAGARGKGSKVTNAKAGRRL*ARRSC WEAWM*GGWGFVAGGSVVRLCQERLNTAGGQSL SSGGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNR LLSGIASQRVTHLLLRNGFIANR*RG*FRQGMH LECFPSARGSPC*GAGAGQGAVTGW*SQVEDRS DRCGCPGVDVWWRWSSELR*AAANAC*A*TWAGS FRWVAGLKSQGQPRHCPGPSLLAPDACACFQEH QIHAAADSEPHSLAQALSAQASGPPWDPGRPGP ASEVLAFVKAGPCRDRWAGPAPGPWASNPAADHR PVRGWTRWGLAGPEG*EGEAAAAKANRSPAPGS CGPGRLPPAGSQRPGSLQG*LPGPVGADVLWLH SLP*HLPRRAGEAGAGGAAAGSRAWFASSAACLH HCGPRAGRR*SHGPLRPGLPPKTVGSDRLHQTG CPG*SQLPRVLQCRPQG*GPGLHRGPLHCHLPA QP*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCP VLSHCSLGPIIKRAAF

Sequence ECGF1.kAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGTGCGTGGGGAGGGT TGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGCCCCACTACCAGCACCGGCCCCAGGGTGCCCCA CCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCT GACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAG GTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAGACGGGAGCCTATACCGCACATGCAGCAACCAG TCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAAGACCCTACCAAGGCTTCTCCCCACCCTGCTCCCCAGCACAGTTCTCCCCACC CCGTTCCCCAGCACAGCGCTTGGGGCCCCTCTGGCTCCAGACCAGGCCCCTTGGAGCAGGAAAAAGATCCACTGATGGAATTCAGACCCCTTTCCCCTTG GGTCCCCAGACAGCTCCCCCAAGGGAGGAGCTGAGGACTTTCCTCCCTCTGCCCCAAGCCTTGTTTCCCCAAGGACAGGTACCAACCTCCTCCCCTACTG ACACTTCTCAACCAAGAAAACTTCCTTTCCATTCCCTCACCAGCTGGGCACCCCTATAGCTGCTTAAATACTTTCCAAATCCAGCTGCACTCCTAGCCAG GGAAGGTGAAGGGATGCACAGAGGTGGGGGAGGGGTACTGTGCAGGGTACTCAGCATCCCTGACCACCAGGTGCCAATGATCAGCGGACGTGGTCTGGGG CACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGGTACGGGGCGCCACGGATCAGTCATTAATCC AGGTTGATGATGGAGACCCTGGCCAGAATCACTAAAAGATCACTGGTGGATCATTAGGGTCACTAATGAGAACACTGGTCAAGGTTACTCATGAGTCACT GGGCCTGGGCCGAAATCATCAGTGGAACTTTGATTAGGATCATAAAATGGGAAGTTGGTCAAAATCACAGATGGCTGGCGGGGCACGGTGGCTCACACCT GTAGTCCTAGCACTTGGGGAGGCCGAAGAGGGCAGATCCCTTGAACCCAGGAGTTCAAAACCAGCCTGGATAACACGGCAAAACCCCATCTCTACAAAAT AGTTCGCTGCGTGTGGTGGTGCACGCATGTGGTTCCAGCTACTCAGGAGGCTGAGGCAGGAGGATCACTTGAGCCTGGGAGGTCTAGGCTGCAGTGAGCC GGGACGATGCCACTGCACTCCAGCCTGGGCAACAGAGTGAGACCCTGTCCCAGCACTCTGGGAGGCAGAGGAGCCCAGTTGGAGATCAGCCTGGGTAATA TAGTGAAACTTGATCTCTACAAAAAAAAGAAGAAAAAAAAAAGCCGCGTGTGGTGGTGCGCACCTGTAGTCCCAGCTACTGGGAAGCTGAGGTGGGAGGA TCACTTAAGCCCAGGAGGCAGAGGTCACAATGAGCCGAAATTGTGCCAACTGCACTCCAGCCTGGGCAACAGAGGAAGACTCTTCACAGAAAAAAAAAAA AAAAAAAAAAAAGCTGCTAAGTCATTTACCATAAGTCACTGAGAACAGGGGATGTCTGACCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCG TGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGGTGACCT GACTCCATGGCCTGCTTCTGCATGTTCACAGGCTCCTGACCTCCAAACTCAAGTCAAGGGCCTCTCGTTAGGAGTTACCCGTCACCTGACCGTGTGCCCC CCTACCCCCATCACAAGATGCCTGACCACCACCATGTGGGGTGGCCTGATACTCAACCCACCAGGTGCTGCCACCCCCATAATAAGGGACTTGACCCTCA ATGCTCAGGGCCCCTGACCCCAAAGTCGGCATCCCCGAACTCTCCCAAGAAGCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCT CCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGA GCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Alternative acceptor

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK RDGGRLSEADIRGFVAAVVNGSAQGAQIGAWGG LGVPDPDWEVSPRDFGSLGVRRCPTTSTGPRVPH RCGLPPSRVPPHTRGHADGHPTSGHGSGGDLGA DPGPGSVGTAAGVARGLAPAACGQAFHRGCG*Q GQPGPRTCPGGMWLQG*KPPPFQTGAYTAHAATS PSTGSSQPQAWPKASKTLPRLLPTLLPSTVLPT PFPSTALGAPLAPDQAPWSRKKIH*WNSDPFPL GSPDSSPKGGAEDFPPSAPSLVSPRTGTNLLPY* HFSTKKTSFPFPHQLGTPIAA*ILSKSSCTPSQ GR*RDAQRWGRGTVQGTQHP*PPGANDQRTWSG AHRRHLG*AGVYSWIQCHPEPRAGTGRHGSVINP G**WRPWPESLKDHWWIIRVTNENTGQGYS*VT GPGPKSSVEL*LGS*NGKLVKITDGWRGTVAHT CSPSTWGGRRGQIP*TQEFKTSLDNTAKPHLYKI VRCVWWCTHVVPATQEAEAGGSLEPGRSRLQ*A GTMPLHSSLGNRVRPCPSTLGGRGAQLEISLGN IVKLDLYKKKKKKKSRVWWCAPVVPATGKLRWED HLSPGGRGHNEPKLCQLHSSLGNRGRLFTEKKK KKKKAAKSFTISH*EQGMSDQMQVLLDQAGCCI VGQSEQLVPADGILYAARDVTATVDSLPLITGDL TPWPASACSQAPDLQTQVKGLSLGVTRHLTVCP PTPITRCLTTTMWGGLILNPPGAATPIIRDLTL NAQGP*PQSRHPRTLPRSSRFSIVSNLLCLPQSL HSQ*ETRGGAVRSGGGR*VRRGRRLPQPGAGPG AGKDAGWRGSQPRASGRGSADRHG

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGIPAGPERWQP**PREP GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S ETEAA*AKRTSGASWPLW*MGARRAHRSVRGEG WAFLTPTGRSARETLGPWGCDGAPLPAPAPGCP TAVGCHPHAYPHIPGAMLMAIRLRGMDLEETSVL TQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDK VSLVLAPALAACGCKVRNHLLSRREPIPHMQQP VHPQAAPNLKPGPKPPRPYQGFSPPCSPAQFSPP RSPAQRLGPLWLQTRPLGAGKRSTDGIQTPFPL GPQTAPPREELRTFLPLPQALFPQGQVPTSSPT DTSQPRKLPFHSLTSWAPL*LLKYFPNPAALLAR EGEGMHRGGGGVLCRVLSIPDHQVPMISGRGLG HTGGTLDKLESIPGFNVIQSPEQVRGATDQSLI QVDDGDPGQNH*KITGGSLGSLMRTLVKVTHESL GLGRNHQWNFD*DHKMGSWSKSQMAGGARWLTP VVLALGEAEEGRSLEPRSSKPAWITRQNPISTK *FAACGGARMWFQLLRRLRQEDHLSLGGLGCSEP GRCHCTPAWATE*DPVPALWEAEEPSWRSAWVI **NLISTKKRRKKKAACGGAHL*SQLLGS*GGR IT*AQEAEVTMSRNCANCTPAWATEEDSSQKKKK KKKKLLSHLP*VTENRGCLTRCKCCWTRRAAVS WVRVSSWFLRTESYMQPEM*QPPWTACHSSQVT *LHGLLLHVHRLLTSKLKSRASR*ELPVT*PCAP LPPSQDA*PPPCGVA*YSTHQVLPPP**GT*PS MLRAPDPKVGIPELSQEAPGSPLSPTSSASPKA SILSKKLVEGLSALVVDVKFGGAAVFPNQEQARE LAKTLVGVGASLGLRVAAALTAM

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE ARRRPPERSGHQGLRGRCGEWERAGRTDRCVGRV GRS*PRLGGQPERLWVPGGATVPHYQHRPQGAP PLWAATLTRTPTYQGPC*WPSDFGAWIWRRPRC *PRPWLSRDSSWSGQRPGASSLWTSIPQGVWVTR SAWSSHLPWRHVAARLETTSFPDGSLYRTCSNQ SIHRQLPTSSLAQSLQDPTKASPHPAPQHSSPH PVPQHSAWGPSGSRPGPLEQEKDPLMEFRPLSPW VPRQLPQGRS*GLSSLCPKPCFPKDRYQPPPLL TLLNQENFLSIPSPAGHPYSCLNTFQIQLHS*P GKVKGCTEVGEGYCAGYSASLTTRCQ*SADVVWG TQEAPWISWSLFLDSMSSRAQSRYGAPRISH*S RLMMETLARITKRSLVDH*GH**EHWSRLLMSH WAWAEIISGTLIRIIKWEVGQNHRWLAGHGGSHL *S*HLGRPKRADPLNPGVQNQPG*HGKTPSLQN SSLRVVVHACGSSYSGG*GRRIT*AWEV*AAVS RDDATALQPGQQSETLSQHSGRQRSPVGDQPG*Y SET*SLQKKEEKKKPRVVVRTCSPSYWEAEVGG SLKPRRQRSQ*AEIVPTALQPGQQRKTLHRKKK KKKKSC*VIYHKSLRTGDV*PDASAAGPGGLLYR GSE*AAGSCGRNPICSQRCDSHRGQPATHHR*P DSMACFCMFTGS*PPNSSQGPLVRSYPSPDRVP PYPHHKMPDHHHVGWPDTQPTRCCHPHNKGLDPQ CSGPLTPKSASPNSPKKLQVLHCLQPPLPPPKP PFSVRNSWRGCPLWWWTLSSEGPPSSPTRSRPG SWQRRWLAWEPA*GFGSRQR*PPW

Sequence ECGF1.cAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGG CCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCA GCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATC AGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGC TGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAG CCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCC AACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCC TGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGG GGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTC GAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGG AGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCG CGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGAC GGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCG TTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGA TGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGC GCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGAC GCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACT GCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCC AAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAAC CGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGG GGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGG TTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCT GCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAG TTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTC ACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTT TGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGC AGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCC CCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCA GGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCC TGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGG ATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCT GGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTT GAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGT ACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTA CGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCA TCATTAAACGGGCTGCGTTTAA

ECGF1.F3 (forward primer)
ECGF1.R30 (reverse primer)
Alternative acceptor

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK RDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLM AIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQ QLVDKHSTGGVGDKVSLVLAPALAACGCKVPMI SGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVL LDQAGCCIVGQSEQLVPADGILYAARDVTATVDS LPLITASILSKKLVEGLSALVVDVKFGGAAVFP NQEQARELAKTLVGVGASLGLRVAAALTAMDKP LGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLG GALLWLSGHAGTQAQGAARVAAALDDGSALGRF ERMLAAQGVDPGLARALCSGSPAERRQLLPRAR EQEELLAPADGTVELVRALPLALVLHELGAGRSR AGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRD GPALSGPQSRALQEALVLSDRAPFAAPSPFAEL VLPPQQ*SSFAAKPCQCLGGSGRIQGCGGGGRLD EHVTPGGLRLPRTR*EPVSGCARVRMRSSGDAC ALPVSVWRPLSLSRRGRAQGAGGRRFLSCLVH* RAAPPRGADAGAGRGGGSPEDSLDSGVVSQLRLL RGAGGAHPRARWRPERGWGARGRLPAGTGVLAA KHSYNALARRFEWSWNLSGHEGAGLAGEGTNPE PPGRGCSESSGGCPQQEGAVTGSPETST*QTFCG GPWERLKGHQRKGRASAVSPEELLGSLDVRRVG FCGGWKCRASLPGEVKHSRRAESELRG*VVQVF CWECGGRPRLVEVAGGNRKVLEESVALGDCKPES YPPPFKKWVYCK*ITWLVQARHALGMLSVSKRF TLLRCRCRAGCGDRLVIPGRGQE*QVWLPRCGC LVEVEF*AQVSSCKCLLSLNVGWVLQMGGWSQVA GAAQALSWAFPSGS*RLCLFPGASDPCCC*LGA PQLGTGSLSSSLGSSLGPWEARPCI*GPGFCQG RALQRQVGRASPRALGFEPGC*SQACSGLDSVGP GWP*GLRRRGCSSKSEQKPCARQLWARATSTCW ITEAGLAARLTSGASGC*CTLASLTALTSAQTS WRSWCRWCGSWKQSLVCLQCSLSSSLWTPSGTTL KPWPATSRTSTQDCWV*PAPPNRLPRLVTVTAC TTMQAPRMRTRTTSWTTPLPSTCSTLTASSRIT TAGADRLSRSQTVCGGTWRLSAVSCLEPLQSGPH H*TGCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG TWAGIRSKRARAAP*AGIPAGPERWQP**PREP GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S ETEAA*AKRTSGASWPLW*MGARRAHRSGPC*W PSDFGAWIWRRPRC*PRPWLSRDSSWSGQRPGA SSLWTSIPQGVWVTRSAWSSHLPWRHVAARCQ*S ADVVWGTQEAPWISWSLFLDSMSSRAQSRCKCC WTRRAAVSWVRVSSWFLRTESYMQPEM*QPPWT ACHSSQPPFSVRNSWRGCPLWWWTLSSEGPPSSP TRSRPGSWQRRWLAWEPA*GFGSRQR*PPWTSP WVAAWATPWRWRRRCSAWTAQARQT*GTWSPRS GAPCSGSADTRGLRPRALPGWPRRWTTARPLAAS SGCWRRRAWIPVWPEPCAREVPQNAGSCCLAPG SRRSCWRPQMAPWSWSGRCRWRWCCTSSGPGAA ALGSRSAWGWAQSCWSTWVRGCAVGPPGSACTGT APRSAARRAAPCRRRSYSPTARHSPPPRPSQSS FCRRSNKAPLPRNLVSAWAGAEGSRAAEAGAVS MNT*PPAGSAFRARAESLSAAAPVCACAAPGTPA PCL*ACGARFP*AGGAERRELEVGASSRAWSTD ARPRREVRTPGLGGEEVALRTRWTPG*FPSSGY CAGLAGHTPGRAGGRSEAGAPVGGSQQAPVFSRP STVITRSRGASSGPGTFLATRALALLGRAQTQN RPGGGAVSPREGALSRRGQ*PGVLRPPPSKPSA GARGKGSKVTNAKAGRRL*ARRSCWEAWM*GGWG FVAGGSVVRLCQERLNTAGGQSLSSGGRSCRFS AGSVEEGRGWLKWLEVTGKCWRNRLLSGIASQR VTHLLLRNGFIANR*RG*FRQGMHLECFPSARGS PC*GAGAGQGAVTGW*SQVEDRSDRCGCPGVDV WWRWSSELR*AAANAC*A*TWAGSFRWVAGLKS QGQPRHCPGPSLLAPDACACFQEHQIHAAADSEP HSLAQALSAQASGPPWDPGRPGPASEVLAFVKA GPCRDRWAGPAPGPWASNPAADHRPVRGWTRWG LAGPEG*EGEAAAAKANRSPAPGSCGPGRLPPAG SQRPGSLQG*LPGPVGADVLWLHSLP*HLPRRA GEAGAGGAAAGSRAWFASSAACLHHCGPRAGRR *SHGPLRPGLPPKTVGSDRLHQTGCPG*SQLPRV LQCRPQG*GPGLHRGPLHCHLPAQP*RPLHGLL RPEQIG*ADLRQCAAAHGGFPQCPVLSHCSLGP IIKRAAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE ARRRPPERSGHQGLRGRCGEWERAGRTDRGHADG HPTSGHGSGGDLGADPGPGSVGTAAGVARGLAP AACGQAFHRGCG*QGQPGPRTCPGGMWLQGAND QRTWSGAHRRHLG*AGVYSWIQCHPEPRADASAA GPGGLLYRGSE*AAGSCGRNPICSQRCDSHRGQ PATHHSLHSQ*ETRGGAVRSGGGR*VRRGRRLP QPGAGPGAGKDAGWRGSQPRASGRGSADRHGQAP GSLRGPRPGGGGGAALHGRRRPARLKGPGHHAR GRPALAQRTRGDSGPGRCPGGRGAGRRLGPWPL RADAGGAGRGSRSGPSPVLGKSRRTPAAAASRPG AGGAAGARRWHRGAGPGAAAGAGAARARGRAQP RWGAAPPGGGRRAAGRRGSEAAPWDPLAPRAPG RPRAQRPAEPRPAGGARTLRPRAIRRPLALRRAR SAAAAIKLLCRETLSVLGRERKDPGLRRRGPSR *TRDPRRAPPSAHALRACQRLRPCAHAQLRGRL RPACERVAPAFPEPAGQSAGSWRSALPLVLGPLT RGPAARCGRRGWEGRR*P*GLAGLRGSFPAPAT ARGWRGTPQGALEAGARLGRPWEAPSRHRCSRG QAQL*RARAALRVVLEPFWPRGRWPCWGGHKPRT ARAGVQ*VLGRVPSAGGGSDRES*DLHLANLLR GPVGKAQRSPTQRQGVGCEPGGAAGKPGCEEGG VLWRVEVSCVSARRG*TQPAGRV*APGVGRAGFL LGVWRKAAVG*SGWR*QESVGGIGCSRGLQARE LPTSF*EMGLLQIDNVVSSGKACTWNAFRQQEV HLAEVQVQGRVR*QAGDPR*RTGVTGVVAQVWMF GGGGVLSSGEQLQMPVKPERGLGPSDGWLVSSR RGSPGTVLGLPFWLLTPVLVSRSIRSMLLLTRS PTAWHRLSQLKPRVLPGTLGGQALHLRSWLLSRQ GPAETGGQGQPQGPGLRTRLLITGLFGAGLGGA WLALRAEKERLQQQKRTEALRQAAVGQGDFHLL DHRGRARCKADFRGQWVLMYFGFTHCPDICPDEL EKLVQVVRQLEAEPGLPPVQPVFITVDPERDDV EAMARYVQDFHPRLLGLTGSTKQVAQASHSYRV YYNAGPKDEDQDYIVDHSIAIYLLNPDGLFTDYY GRSRSAEQISDSVRRHMAAFRSVLS*ATAVWAP SLNGLRL