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Sequences

Sequence ECGF1.kAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT
ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG
GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG
CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGTGCGTGGGGAGGGT
TGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCCTGGGGGTGCGACGGTGCCCCACTACCAGCACCGGCCCCAGGGTGCCCCA
CCGCTGTGGGCTGCCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCT
GACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAG
GTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTTAGAAACCACCTCCTTTCCAGACGGGAGCCTATACCGCACATGCAGCAACCAG
TCCATCCACAGGCAGCTCCCAACCTCAAGCCTGGCCCAAAGCCTCCAAGACCCTACCAAGGCTTCTCCCCACCCTGCTCCCCAGCACAGTTCTCCCCACC
CCGTTCCCCAGCACAGCGCTTGGGGCCCCTCTGGCTCCAGACCAGGCCCCTTGGAGCAGGAAAAAGATCCACTGATGGAATTCAGACCCCTTTCCCCTTG
GGTCCCCAGACAGCTCCCCCAAGGGAGGAGCTGAGGACTTTCCTCCCTCTGCCCCAAGCCTTGTTTCCCCAAGGACAGGTACCAACCTCCTCCCCTACTG
ACACTTCTCAACCAAGAAAACTTCCTTTCCATTCCCTCACCAGCTGGGCACCCCTATAGCTGCTTAAATACTTTCCAAATCCAGCTGCACTCCTAGCCAG
GGAAGGTGAAGGGATGCACAGAGGTGGGGGAGGGGTACTGTGCAGGGTACTCAGCATCCCTGACCACCAGGTGCCAATGATCAGCGGACGTGGTCTGGGG
CACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGGTACGGGGCGCCACGGATCAGTCATTAATCC
AGGTTGATGATGGAGACCCTGGCCAGAATCACTAAAAGATCACTGGTGGATCATTAGGGTCACTAATGAGAACACTGGTCAAGGTTACTCATGAGTCACT
GGGCCTGGGCCGAAATCATCAGTGGAACTTTGATTAGGATCATAAAATGGGAAGTTGGTCAAAATCACAGATGGCTGGCGGGGCACGGTGGCTCACACCT
GTAGTCCTAGCACTTGGGGAGGCCGAAGAGGGCAGATCCCTTGAACCCAGGAGTTCAAAACCAGCCTGGATAACACGGCAAAACCCCATCTCTACAAAAT
AGTTCGCTGCGTGTGGTGGTGCACGCATGTGGTTCCAGCTACTCAGGAGGCTGAGGCAGGAGGATCACTTGAGCCTGGGAGGTCTAGGCTGCAGTGAGCC
GGGACGATGCCACTGCACTCCAGCCTGGGCAACAGAGTGAGACCCTGTCCCAGCACTCTGGGAGGCAGAGGAGCCCAGTTGGAGATCAGCCTGGGTAATA
TAGTGAAACTTGATCTCTACAAAAAAAAGAAGAAAAAAAAAAGCCGCGTGTGGTGGTGCGCACCTGTAGTCCCAGCTACTGGGAAGCTGAGGTGGGAGGA
TCACTTAAGCCCAGGAGGCAGAGGTCACAATGAGCCGAAATTGTGCCAACTGCACTCCAGCCTGGGCAACAGAGGAAGACTCTTCACAGAAAAAAAAAAA
AAAAAAAAAAAAGCTGCTAAGTCATTTACCATAAGTCACTGAGAACAGGGGATGTCTGACCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCG
TGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGGTGACCT
GACTCCATGGCCTGCTTCTGCATGTTCACAGGCTCCTGACCTCCAAACTCAAGTCAAGGGCCTCTCGTTAGGAGTTACCCGTCACCTGACCGTGTGCCCC
CCTACCCCCATCACAAGATGCCTGACCACCACCATGTGGGGTGGCCTGATACTCAACCCACCAGGTGCTGCCACCCCCATAATAAGGGACTTGACCCTCA
ATGCTCAGGGCCCCTGACCCCAAAGTCGGCATCCCCGAACTCTCCCAAGAAGCTCCAGGTTCTCCATTGTCTCCAACCTCCTCTGCCTCCCCCAAAGCCT
CCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGA
GCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGA
  • ECGF1.F3 (forward primer)
  • ECGF1.R30 (reverse primer)
  • Alternative acceptor

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG
YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG
APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK
RDGGRLSEADIRGFVAAVVNGSAQGAQIGAWGG
LGVPDPDWEVSPRDFGSLGVRRCPTTSTGPRVPH
RCGLPPSRVPPHTRGHADGHPTSGHGSGGDLGA
DPGPGSVGTAAGVARGLAPAACGQAFHRGCG*Q
GQPGPRTCPGGMWLQG*KPPPFQTGAYTAHAATS
PSTGSSQPQAWPKASKTLPRLLPTLLPSTVLPT
PFPSTALGAPLAPDQAPWSRKKIH*WNSDPFPL
GSPDSSPKGGAEDFPPSAPSLVSPRTGTNLLPY*
HFSTKKTSFPFPHQLGTPIAA*ILSKSSCTPSQ
GR*RDAQRWGRGTVQGTQHP*PPGANDQRTWSG
AHRRHLG*AGVYSWIQCHPEPRAGTGRHGSVINP
G**WRPWPESLKDHWWIIRVTNENTGQGYS*VT
GPGPKSSVEL*LGS*NGKLVKITDGWRGTVAHT
CSPSTWGGRRGQIP*TQEFKTSLDNTAKPHLYKI
VRCVWWCTHVVPATQEAEAGGSLEPGRSRLQ*A
GTMPLHSSLGNRVRPCPSTLGGRGAQLEISLGN
IVKLDLYKKKKKKKSRVWWCAPVVPATGKLRWED
HLSPGGRGHNEPKLCQLHSSLGNRGRLFTEKKK
KKKKAAKSFTISH*EQGMSDQMQVLLDQAGCCI
VGQSEQLVPADGILYAARDVTATVDSLPLITGDL
TPWPASACSQAPDLQTQVKGLSLGVTRHLTVCP
PTPITRCLTTTMWGGLILNPPGAATPIIRDLTL
NAQGP*PQSRHPRTLPRSSRFSIVSNLLCLPQSL
HSQ*ETRGGAVRSGGGR*VRRGRRLPQPGAGPG
AGKDAGWRGSQPRASGRGSADRHG

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG
TWAGIRSKRARAAP*AGIPAGPERWQP**PREP
GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S
ETEAA*AKRTSGASWPLW*MGARRAHRSVRGEG
WAFLTPTGRSARETLGPWGCDGAPLPAPAPGCP
TAVGCHPHAYPHIPGAMLMAIRLRGMDLEETSVL
TQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDK
VSLVLAPALAACGCKVRNHLLSRREPIPHMQQP
VHPQAAPNLKPGPKPPRPYQGFSPPCSPAQFSPP
RSPAQRLGPLWLQTRPLGAGKRSTDGIQTPFPL
GPQTAPPREELRTFLPLPQALFPQGQVPTSSPT
DTSQPRKLPFHSLTSWAPL*LLKYFPNPAALLAR
EGEGMHRGGGGVLCRVLSIPDHQVPMISGRGLG
HTGGTLDKLESIPGFNVIQSPEQVRGATDQSLI
QVDDGDPGQNH*KITGGSLGSLMRTLVKVTHESL
GLGRNHQWNFD*DHKMGSWSKSQMAGGARWLTP
VVLALGEAEEGRSLEPRSSKPAWITRQNPISTK
*FAACGGARMWFQLLRRLRQEDHLSLGGLGCSEP
GRCHCTPAWATE*DPVPALWEAEEPSWRSAWVI
**NLISTKKRRKKKAACGGAHL*SQLLGS*GGR
IT*AQEAEVTMSRNCANCTPAWATEEDSSQKKKK
KKKKLLSHLP*VTENRGCLTRCKCCWTRRAAVS
WVRVSSWFLRTESYMQPEM*QPPWTACHSSQVT
*LHGLLLHVHRLLTSKLKSRASR*ELPVT*PCAP
LPPSQDA*PPPCGVA*YSTHQVLPPP**GT*PS
MLRAPDPKVGIPELSQEAPGSPLSPTSSASPKA
SILSKKLVEGLSALVVDVKFGGAAVFPNQEQARE
LAKTLVGVGASLGLRVAAALTAM

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV
PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR
GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE
ARRRPPERSGHQGLRGRCGEWERAGRTDRCVGRV
GRS*PRLGGQPERLWVPGGATVPHYQHRPQGAP
PLWAATLTRTPTYQGPC*WPSDFGAWIWRRPRC
*PRPWLSRDSSWSGQRPGASSLWTSIPQGVWVTR
SAWSSHLPWRHVAARLETTSFPDGSLYRTCSNQ
SIHRQLPTSSLAQSLQDPTKASPHPAPQHSSPH
PVPQHSAWGPSGSRPGPLEQEKDPLMEFRPLSPW
VPRQLPQGRS*GLSSLCPKPCFPKDRYQPPPLL
TLLNQENFLSIPSPAGHPYSCLNTFQIQLHS*P
GKVKGCTEVGEGYCAGYSASLTTRCQ*SADVVWG
TQEAPWISWSLFLDSMSSRAQSRYGAPRISH*S
RLMMETLARITKRSLVDH*GH**EHWSRLLMSH
WAWAEIISGTLIRIIKWEVGQNHRWLAGHGGSHL
*S*HLGRPKRADPLNPGVQNQPG*HGKTPSLQN
SSLRVVVHACGSSYSGG*GRRIT*AWEV*AAVS
RDDATALQPGQQSETLSQHSGRQRSPVGDQPG*Y
SET*SLQKKEEKKKPRVVVRTCSPSYWEAEVGG
SLKPRRQRSQ*AEIVPTALQPGQQRKTLHRKKK
KKKKSC*VIYHKSLRTGDV*PDASAAGPGGLLYR
GSE*AAGSCGRNPICSQRCDSHRGQPATHHR*P
DSMACFCMFTGS*PPNSSQGPLVRSYPSPDRVP
PYPHHKMPDHHHVGWPDTQPTRCCHPHNKGLDPQ
CSGPLTPKSASPNSPKKLQVLHCLQPPLPPPKP
PFSVRNSWRGCPLWWWTLSSEGPPSSPTRSRPG
SWQRRWLAWEPA*GFGSRQR*PPW

Sequence ECGF1.cAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT
ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGG
GGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAG
CGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGGGCCATGCTGATGG
CCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCA
GCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTGCCAATGATC
AGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGC
TGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAG
CCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGTTCGGAGGGGCCGCCGTCTTCCCC
AACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCC
TGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGGACCTGGTCACCACGCTCGG
GGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTC
GAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTCGCGCCCGGG
AGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCG
CGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGAC
GGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGCCCCCTCGCCCTTCGCAGAGCTCG
TTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGCTGCGGAGGCGGGGGCCGTCTCGA
TGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCATGCGCAGCTCCGGGGACGCCTGC
GCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTTCCTCTCGTGCTTGGTCCACTGAC
GCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGGGTAGTTTCCCAGCTCCGGCTACT
GCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAGCAGGCACCGGTGTTCTCGCGGCC
AAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGCTGGGGAGGGCACAAACCCAGAAC
CGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACCTCCACCTAGCAAACCTTCTGCGG
GGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAAGCCTGGATGTGAGGAGGGTGGGG
TTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCGGGGGTAGGTCGTGCAGGTTTTCT
GCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCTCTCGGGGATTGCAAGCCAGAGAG
TTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAATGCTTTCCGTCAGCAAGAGGTTC
ACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGTGTGGTTGCCCAGGTGTGGATGTT
TGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAGATGGGTGGCTGGTCTCAAGTCGC
AGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATCCATGCTGCTGCTGACTCGGAGCC
CCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTGAGGTCCTGGCTTTTGTCAAGGCA
GGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTCGGGGCTGGACTCGGTGGGGCC
TGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGGCCAGGGCGACTTCCACCTGCTGG
ATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGCCCTGACATCTGCCCAGACGAGCT
GGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTGTGGACCCCGAGCGGGACGACGTT
GAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCAGGCTAGTCACAGTTACCGCGTGT
ACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCTGACGGCCTCTTCACGGATTACTA
CGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTTGAGCCACTGCAGTCTGGGCCCCA
TCATTAAACGGGCTGCGTTTAA
  • ECGF1.F3 (forward primer)
  • ECGF1.R30 (reverse primer)
  • Alternative acceptor

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG
YLGWDPEQAGEGSALSRHPRRPGAMAALMTPGTG
APPAPGDFSGEGSQGLPDPSPEPKQLPELIRMK
RDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLM
AIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQ
QLVDKHSTGGVGDKVSLVLAPALAACGCKVPMI
SGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVL
LDQAGCCIVGQSEQLVPADGILYAARDVTATVDS
LPLITASILSKKLVEGLSALVVDVKFGGAAVFP
NQEQARELAKTLVGVGASLGLRVAAALTAMDKP
LGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLG
GALLWLSGHAGTQAQGAARVAAALDDGSALGRF
ERMLAAQGVDPGLARALCSGSPAERRQLLPRAR
EQEELLAPADGTVELVRALPLALVLHELGAGRSR
AGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRD
GPALSGPQSRALQEALVLSDRAPFAAPSPFAEL
VLPPQQ*SSFAAKPCQCLGGSGRIQGCGGGGRLD
EHVTPGGLRLPRTR*EPVSGCARVRMRSSGDAC
ALPVSVWRPLSLSRRGRAQGAGGRRFLSCLVH*
RAAPPRGADAGAGRGGGSPEDSLDSGVVSQLRLL
RGAGGAHPRARWRPERGWGARGRLPAGTGVLAA
KHSYNALARRFEWSWNLSGHEGAGLAGEGTNPE
PPGRGCSESSGGCPQQEGAVTGSPETST*QTFCG
GPWERLKGHQRKGRASAVSPEELLGSLDVRRVG
FCGGWKCRASLPGEVKHSRRAESELRG*VVQVF
CWECGGRPRLVEVAGGNRKVLEESVALGDCKPES
YPPPFKKWVYCK*ITWLVQARHALGMLSVSKRF
TLLRCRCRAGCGDRLVIPGRGQE*QVWLPRCGC
LVEVEF*AQVSSCKCLLSLNVGWVLQMGGWSQVA
GAAQALSWAFPSGS*RLCLFPGASDPCCC*LGA
PQLGTGSLSSSLGSSLGPWEARPCI*GPGFCQG
RALQRQVGRASPRALGFEPGC*SQACSGLDSVGP
GWP*GLRRRGCSSKSEQKPCARQLWARATSTCW
ITEAGLAARLTSGASGC*CTLASLTALTSAQTS
WRSWCRWCGSWKQSLVCLQCSLSSSLWTPSGTTL
KPWPATSRTSTQDCWV*PAPPNRLPRLVTVTAC
TTMQAPRMRTRTTSWTTPLPSTCSTLTASSRIT
TAGADRLSRSQTVCGGTWRLSAVSCLEPLQSGPH
H*TGCV*

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG
TWAGIRSKRARAAP*AGIPAGPERWQP**PREP
GPHPRLVTSPGKGARDFPTLRQSPSSSRS*SA*S
ETEAA*AKRTSGASWPLW*MGARRAHRSGPC*W
PSDFGAWIWRRPRC*PRPWLSRDSSWSGQRPGA
SSLWTSIPQGVWVTRSAWSSHLPWRHVAARCQ*S
ADVVWGTQEAPWISWSLFLDSMSSRAQSRCKCC
WTRRAAVSWVRVSSWFLRTESYMQPEM*QPPWT
ACHSSQPPFSVRNSWRGCPLWWWTLSSEGPPSSP
TRSRPGSWQRRWLAWEPA*GFGSRQR*PPWTSP
WVAAWATPWRWRRRCSAWTAQARQT*GTWSPRS
GAPCSGSADTRGLRPRALPGWPRRWTTARPLAAS
SGCWRRRAWIPVWPEPCAREVPQNAGSCCLAPG
SRRSCWRPQMAPWSWSGRCRWRWCCTSSGPGAA
ALGSRSAWGWAQSCWSTWVRGCAVGPPGSACTGT
APRSAARRAAPCRRRSYSPTARHSPPPRPSQSS
FCRRSNKAPLPRNLVSAWAGAEGSRAAEAGAVS
MNT*PPAGSAFRARAESLSAAAPVCACAAPGTPA
PCL*ACGARFP*AGGAERRELEVGASSRAWSTD
ARPRREVRTPGLGGEEVALRTRWTPG*FPSSGY
CAGLAGHTPGRAGGRSEAGAPVGGSQQAPVFSRP
STVITRSRGASSGPGTFLATRALALLGRAQTQN
RPGGGAVSPREGALSRRGQ*PGVLRPPPSKPSA
GARGKGSKVTNAKAGRRL*ARRSCWEAWM*GGWG
FVAGGSVVRLCQERLNTAGGQSLSSGGRSCRFS
AGSVEEGRGWLKWLEVTGKCWRNRLLSGIASQR
VTHLLLRNGFIANR*RG*FRQGMHLECFPSARGS
PC*GAGAGQGAVTGW*SQVEDRSDRCGCPGVDV
WWRWSSELR*AAANAC*A*TWAGSFRWVAGLKS
QGQPRHCPGPSLLAPDACACFQEHQIHAAADSEP
HSLAQALSAQASGPPWDPGRPGPASEVLAFVKA
GPCRDRWAGPAPGPWASNPAADHRPVRGWTRWG
LAGPEG*EGEAAAAKANRSPAPGSCGPGRLPPAG
SQRPGSLQG*LPGPVGADVLWLHSLP*HLPRRA
GEAGAGGAAAGSRAWFASSAACLHHCGPRAGRR
*SHGPLRPGLPPKTVGSDRLHQTGCPG*SQLPRV
LQCRPQG*GPGLHRGPLHCHLPAQP*RPLHGLL
RPEQIG*ADLRQCAAAHGGFPQCPVLSHCSLGP
IIKRAAF

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV
PGLGSGASGRGQRPKQASPQARSDGSLDDPGNR
GPTRAW*LLRGREPGTSRPFARAQAAPGADPHE
ARRRPPERSGHQGLRGRCGEWERAGRTDRGHADG
HPTSGHGSGGDLGADPGPGSVGTAAGVARGLAP
AACGQAFHRGCG*QGQPGPRTCPGGMWLQGAND
QRTWSGAHRRHLG*AGVYSWIQCHPEPRADASAA
GPGGLLYRGSE*AAGSCGRNPICSQRCDSHRGQ
PATHHSLHSQ*ETRGGAVRSGGGR*VRRGRRLP
QPGAGPGAGKDAGWRGSQPRASGRGSADRHGQAP
GSLRGPRPGGGGGAALHGRRRPARLKGPGHHAR
GRPALAQRTRGDSGPGRCPGGRGAGRRLGPWPL
RADAGGAGRGSRSGPSPVLGKSRRTPAAAASRPG
AGGAAGARRWHRGAGPGAAAGAGAARARGRAQP
RWGAAPPGGGRRAAGRRGSEAAPWDPLAPRAPG
RPRAQRPAEPRPAGGARTLRPRAIRRPLALRRAR
SAAAAIKLLCRETLSVLGRERKDPGLRRRGPSR
*TRDPRRAPPSAHALRACQRLRPCAHAQLRGRL
RPACERVAPAFPEPAGQSAGSWRSALPLVLGPLT
RGPAARCGRRGWEGRR*P*GLAGLRGSFPAPAT
ARGWRGTPQGALEAGARLGRPWEAPSRHRCSRG
QAQL*RARAALRVVLEPFWPRGRWPCWGGHKPRT
ARAGVQ*VLGRVPSAGGGSDRES*DLHLANLLR
GPVGKAQRSPTQRQGVGCEPGGAAGKPGCEEGG
VLWRVEVSCVSARRG*TQPAGRV*APGVGRAGFL
LGVWRKAAVG*SGWR*QESVGGIGCSRGLQARE
LPTSF*EMGLLQIDNVVSSGKACTWNAFRQQEV
HLAEVQVQGRVR*QAGDPR*RTGVTGVVAQVWMF
GGGGVLSSGEQLQMPVKPERGLGPSDGWLVSSR
RGSPGTVLGLPFWLLTPVLVSRSIRSMLLLTRS
PTAWHRLSQLKPRVLPGTLGGQALHLRSWLLSRQ
GPAETGGQGQPQGPGLRTRLLITGLFGAGLGGA
WLALRAEKERLQQQKRTEALRQAAVGQGDFHLL
DHRGRARCKADFRGQWVLMYFGFTHCPDICPDEL
EKLVQVVRQLEAEPGLPPVQPVFITVDPERDDV
EAMARYVQDFHPRLLGLTGSTKQVAQASHSYRV
YYNAGPKDEDQDYIVDHSIAIYLLNPDGLFTDYY
GRSRSAEQISDSVRRHMAAFRSVLS*ATAVWAP
SLNGLRL

Sequence ECGF1.bAug05

CCCGCCTGCCGTCCTGGGGCGCCGCCGCCCCGCCGCCGGCAGTGGACCGCTGTGCGCGAACCCTGAACCCTACGGTCCCGACCCGCGGGCGAGGCCGGGT
ACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGCAGGCCGCAGACCCCGGACGTGTCAGGCATCCCCGCAGGCCCGGAGCGATGGCA
GCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGC
TCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGC
ACAGATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTG
GAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCAT
GTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAG
CCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGA
GATGTGACAGCCACCGTGGACAGCCTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACGTTAAGT
TCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGGCGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGC
GCTGACCGCCATGGACAAGCCCCTGGGTCGCTGCGTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTA
AGGGACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGCTGCCCGGGTGGCCGCGGCGCTGGACG
ACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAGGGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCG
GCAGCTGCTGCCTCGCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCTGGCGCTGGTGCTGCAC
GAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGCGCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCC
CCTGGCTCCGCGTGCACCGGGACGGCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATTCGCCGC
CCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAAAGCTCCTTTGCCGCGAAACCTTGTCAGTGCTTGGGCGGGAGCGGAAGGATCCAGGGC
TGCGGAGGCGGGGGCCGTCTCGATGAACACGTGACCCCCGGCGGGCTCCGCCTTCCGCGCACGCGCTGAGAGCCTGTCAGCGGCTGCGCCCGTGTGCGCA
TGCGCAGCTCCGGGGACGCCTGCGCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCCCTGAGCCGGCGGGGCAGAGCGCAGGGAGCTGGAGGTCGGCGCTT
CCTCTCGTGCTTGGTCCACTGACGCGCGGCCCCGCCGCGAGGTGCGGACGCCGGGGCTGGGAGGGGAGGAGGTAGCCCTGAGGACTCGCTGGACTCCGGG
GTAGTTTCCCAGCTCCGGCTACTGCGCGGGGCTGGCGGGGCACACCCCAGGGCGCGCTGGAGGCCGGAGCGAGGCTGGGGCGCCCGTGGGAGGCTCCCAG
CAGGCACCGGTGTTCTCGCGGCCAAGCACAGTTATAACGCGCTCGCGCGGCGCTTCGAGTGGTCCTGGAACCTTTCTGGCCACGAGGGCGCTGGCCTTGC
TGGGGAGGGCACAAACCCAGAACCGCCCGGGCGGGGGTGCAGTGAGTCCTCGGGAGGGTGCCCTCAGCAGGAGGGGGCAGTGACCGGGAGTCCTGAGACC
TCCACCTAGCAAACCTTCTGCGGGGGCCCGTGGGAAAGGCTCAAAGGTCACCAACGCAAAGGCAGGGCGTCGGCTGTGAGCCCGGAGGAGCTGCTGGGAA
GCCTGGATGTGAGGAGGGTGGGGTTTTGTGGCGGGTGGAAGTGTCGTGCGTCTCTGCCAGGAGAGGTTAAACACAGCCGGCGGGCAGAGTCTGAGCTCCG
GGGGTAGGTCGTGCAGGTTTTCTGCTGGGAGTGTGGAGGAAGGCCGCGGTTGGTTGAAGTGGCTGGAGGTAACAGGAAAGTGTTGGAGGAATCGGTTGCT
CTCGGGGATTGCAAGCCAGAGAGTTACCCACCTCCTTTTAAGAAATGGGTTTATTGCAAATAGATAACGTGGTTAGTTCAGGCAAGGCATGCACTTGGAA
TGCTTTCCGTCAGCAAGAGGTTCACCTTGCTGAGGTGCAGGTGCAGGGCAGGGTGCGGTGACAGGCTGGTGATCCCAGGTAGAGGACAGGAGTGACAGGT
GTGGTTGCCCAGGTGTGGATGTTTGGTGGAGGTGGAGTTCTGAGCTCAGGTGAGCAGCTGCAAATGCCTGTTAAGCCTGAACGTGGGCTGGGTCCTTCAG
ATGGGTGGCTGGTCTCAAGTCGCAGGGGCAGCCCAGGCACTGTCCTGGGCCTTCCCTTCTGGCTCCTGACGCCTGTGCTTGTTTCCAGGAGCATCAGATC
CATGCTGCTGCTGACTCGGAGCCCCACAGCTTGGCACAGGCTCTCTCAGCTCAAGCCTCGGGTCCTCCCTGGGACCCTGGGAGGCCAGGCCCTGCATCTG
AGGTCCTGGCTTTTGTCAAGGCAGGGCCCTGCAGAGACAGGTGGGCAGGGCCAGCCCCAGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGT
TCGGGGCTGGACTCGGTGGGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCGAACAGAAGCCCTGCGCCAGGCAGCTGTGGG
CCAGGGCGACTTCCACCTGCTGGATCACAGAGGCCGGGCTCGCTGCAAGGCTGACTTCCGGGGCCAGTGGGTGCTGATGTACTTTGGCTTCACTCACTGC
CCTGACATCTGCCCAGACGAGCTGGAGAAGCTGGTGCAGGTGGTGCGGCAGCTGGAAGCAGAGCCTGGTTTGCCTCCAGTGCAGCCTGTCTTCATCACTG
TGGACCCCGAGCGGGACGACGTTGAAGCCATGGCCCGCTACGTCCAGGACTTCCACCCAAGACTGTTGGGTCTGACCGGCTCCACCAAACAGGTTGCCCA
GGCTAGTCACAGTTACCGCGTGTACTACAATGCAGGCCCCAAGGATGAGGACCAGGACTACATCGTGGACCACTCCATTGCCATCTACCTGCTCAACCCT
GACGGCCTCTTCACGGATTACTACGGCCGGAGCAGATCGGCTGAGCAGATCTCAGACAGTGTGCGGCGGCACATGGCGGCTTTCCGCAGTGTCCTGTCTT
GAGCCACTGCAGTCTGGGCCCCATCATTAAACGGGCTGCGTTTAA
  • ECGF1.F3 (forward primer)
  • ECGF1.R30 (reverse primer)
  • Alternative acceptor

Frame 0

PACRPGAPPPRRRQWTAVREP*TLRSRPAGEAG
YLGWDPEQAGEGSALSRPQTPDVSGIPAGPERWQ
P**PREPGPHPRLVTSPGKGARDFPTLRQSPSS
SRS*SA*SETEAA*AKRTSGASWPLW*MGARRA
HRSGPC*WPSDFGAWIWRRPRC*PRPWLSRDSSW
SGQRPGASSLWTSIPQGVWVTRSAWSSHLPWRH
VAARCQ*SADVVWGTQEAPWISWSLFLDSMSSR
AQSRCKCCWTRRAAVSWVRVSSWFLRTESYMQPE
M*QPPWTACHSSQPPFSVRNSWRGCPLWWWTLS
SEGPPSSPTRSRPGSWQRRWLAWEPA*GFGSRQ
R*PPWTSPWVAAWATPWRWRRRCSAWTAQARQT*
GTWSPRSGAPCSGSADTRGLRPRALPGWPRRWT
TARPLAASSGCWRRRAWIPVWPEPCAREVPQNA
GSCCLAPGSRRSCWRPQMAPWSWSGRCRWRWCCT
SSGPGAAALGSRSAWGWAQSCWSTWVRGCAVGP
PGSACTGTAPRSAARRAAPCRRRSYSPTARHSP
PPRPSQSSFCRRSNKAPLPRNLVSAWAGAEGSRA
AEAGAVSMNT*PPAGSAFRARAESLSAAAPVCA
CAAPGTPAPCL*ACGARFP*AGGAERRELEVGA
SSRAWSTDARPRREVRTPGLGGEEVALRTRWTPG
*FPSSGYCAGLAGHTPGRAGGRSEAGAPVGGSQ
QAPVFSRPSTVITRSRGASSGPGTFLATRALAL
LGRAQTQNRPGGGAVSPREGALSRRGQ*PGVLRP
PPSKPSAGARGKGSKVTNAKAGRRL*ARRSCWE
AWM*GGWGFVAGGSVVRLCQERLNTAGGQSLSS
GGRSCRFSAGSVEEGRGWLKWLEVTGKCWRNRLL
SGIASQRVTHLLLRNGFIANR*RG*FRQGMHLE
CFPSARGSPC*GAGAGQGAVTGW*SQVEDRSDR
CGCPGVDVWWRWSSELR*AAANAC*A*TWAGSFR
WVAGLKSQGQPRHCPGPSLLAPDACACFQEHQI
HAAADSEPHSLAQALSAQASGPPWDPGRPGPAS
EVLAFVKAGPCRDRWAGPAPGPWASNPAADHRPV
RGWTRWGLAGPEG*EGEAAAAKANRSPAPGSCG
PGRLPPAGSQRPGSLQG*LPGPVGADVLWLHSL
P*HLPRRAGEAGAGGAAAGSRAWFASSAACLHHC
GPRAGRR*SHGPLRPGLPPKTVGSDRLHQTGCP
G*SQLPRVLQCRPQG*GPGLHRGPLHCHLPAQP
*RPLHGLLRPEQIG*ADLRQCAAAHGGFPQCPVL
SHCSLGPIIKRAAF

Frame 1

PPAVLGRRRPAAGSGPLCANPEPYGPDPRARPG
TWAGIRSKRARAAP*AGRRPRTCQASPQARSDG
SLDDPGNRGPTRAW*LLRGREPGTSRPFARAQAA
PGADPHEARRRPPERSGHQGLRGRCGEWERAGR
TDRGHADGHPTSGHGSGGDLGADPGPGSVGTAA
GVARGLAPAACGQAFHRGCG*QGQPGPRTCPGGM
WLQGANDQRTWSGAHRRHLG*AGVYSWIQCHPE
PRADASAAGPGGLLYRGSE*AAGSCGRNPICSQ
RCDSHRGQPATHHSLHSQ*ETRGGAVRSGGGR*V
RRGRRLPQPGAGPGAGKDAGWRGSQPRASGRGS
ADRHGQAPGSLRGPRPGGGGGAALHGRRRPARL
KGPGHHARGRPALAQRTRGDSGPGRCPGGRGAGR
RLGPWPLRADAGGAGRGSRSGPSPVLGKSRRTP
AAAASRPGAGGAAGARRWHRGAGPGAAAGAGAA
RARGRAQPRWGAAPPGGGRRAAGRRGSEAAPWDP
LAPRAPGRPRAQRPAEPRPAGGARTLRPRAIRR
PLALRRARSAAAAIKLLCRETLSVLGRERKDPG
LRRRGPSR*TRDPRRAPPSAHALRACQRLRPCAH
AQLRGRLRPACERVAPAFPEPAGQSAGSWRSAL
PLVLGPLTRGPAARCGRRGWEGRR*P*GLAGLR
GSFPAPATARGWRGTPQGALEAGARLGRPWEAPS
RHRCSRGQAQL*RARAALRVVLEPFWPRGRWPC
WGGHKPRTARAGVQ*VLGRVPSAGGGSDRES*D
LHLANLLRGPVGKAQRSPTQRQGVGCEPGGAAGK
PGCEEGGVLWRVEVSCVSARRG*TQPAGRV*AP
GVGRAGFLLGVWRKAAVG*SGWR*QESVGGIGC
SRGLQARELPTSF*EMGLLQIDNVVSSGKACTWN
AFRQQEVHLAEVQVQGRVR*QAGDPR*RTGVTG
VVAQVWMFGGGGVLSSGEQLQMPVKPERGLGPS
DGWLVSSRRGSPGTVLGLPFWLLTPVLVSRSIRS
MLLLTRSPTAWHRLSQLKPRVLPGTLGGQALHL
RSWLLSRQGPAETGGQGQPQGPGLRTRLLITGL
FGAGLGGAWLALRAEKERLQQQKRTEALRQAAVG
QGDFHLLDHRGRARCKADFRGQWVLMYFGFTHC
PDICPDELEKLVQVVRQLEAEPGLPPVQPVFIT
VDPERDDVEAMARYVQDFHPRLLGLTGSTKQVAQ
ASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLNP
DGLFTDYYGRSRSAEQISDSVRRHMAAFRSVLS
*ATAVWAPSLNGLRL

Frame 2

RLPSWGAAAPPPAVDRCARTLNPTVPTRGRGRV
PGLGSGASGRGQRPKQAADPGRVRHPRRPGAMA
ALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQ
LPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGA
QIGAMLMAIRLRGMDLEETSVLTQALAQSGQQL
EWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAA
CGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQS
PEQMQVLLDQAGCCIVGQSEQLVPADGILYAAR
DVTATVDSLPLITASILSKKLVEGLSALVVDVK
FGGAAVFPNQEQARELAKTLVGVGASLGLRVAAA
LTAMDKPLGRCVGHALEVEEALLCMDGAGPPDL
RDLVTTLGGALLWLSGHAGTQAQGAARVAAALD
DGSALGRFERMLAAQGVDPGLARALCSGSPAERR
QLLPRAREQEELLAPADGTVELVRALPLALVLH
ELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGT
PWLRVHRDGPALSGPQSRALQEALVLSDRAPFAA
PSPFAELVLPPQQ*SSFAAKPCQCLGGSGRIQG
CGGGGRLDEHVTPGGLRLPRTR*EPVSGCARVR
MRSSGDACALPVSVWRPLSLSRRGRAQGAGGRRF
LSCLVH*RAAPPRGADAGAGRGGGSPEDSLDSG
VVSQLRLLRGAGGAHPRARWRPERGWGARGRLP
AGTGVLAAKHSYNALARRFEWSWNLSGHEGAGLA
GEGTNPEPPGRGCSESSGGCPQQEGAVTGSPET
ST*QTFCGGPWERLKGHQRKGRASAVSPEELLG
SLDVRRVGFCGGWKCRASLPGEVKHSRRAESELR
G*VVQVFCWECGGRPRLVEVAGGNRKVLEESVA
LGDCKPESYPPPFKKWVYCK*ITWLVQARHALG
MLSVSKRFTLLRCRCRAGCGDRLVIPGRGQE*QV
WLPRCGCLVEVEF*AQVSSCKCLLSLNVGWVLQ
MGGWSQVAGAAQALSWAFPSGS*RLCLFPGASD
PCCC*LGAPQLGTGSLSSSLGSSLGPWEARPCI*
GPGFCQGRALQRQVGRASPRALGFEPGC*SQAC
SGLDSVGPGWP*GLRRRGCSSKSEQKPCARQLW
ARATSTCWITEAGLAARLTSGASGC*CTLASLTA
LTSAQTSWRSWCRWCGSWKQSLVCLQCSLSSSL
WTPSGTTLKPWPATSRTSTQDCWV*PAPPNRLP
RLVTVTACTTMQAPRMRTRTTSWTTPLPSTCSTL
TASSRITTAGADRLSRSQTVCGGTWRLSAVSCL
EPLQSGPHH*TGCV*

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