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Sequences

Sequence GALT.bAug05

GCCTTGGCTGTGTCTGTGGCTTCCTTTCCAGACTTGGGGGAGGCCTCTTCCCTTCCCAGGGCCTTGGGTGGGAGGGAAGGAGGGAGGAGGCAGGAGCAGG GAGGGGGCCTGGCTAGGGGCGGCAGAGCTCCAGGGCTTAGAGGGGGCGGGGCAGGGAAGGGGAAGGAGGGGATGGCTCTGGGGTCCCAGAACTGAGTGGA GTAGGAGACGGGGGCTGTAGCTGGTGAGAGGAAGTCCTAGAGGCTATGGACACTCTGCTGCTGGGATCACCGAGATGAGCAGCAGCTGCTCAGGGCTGAG CAGGGTCCTGGTGGCCGTGGCTACAGCCCTGGTGTCTGCCTCCTCCCCCTGCCCCCAGGCCTGGGGCCCCCCAGGGGTCCAGTATGGGCAGCCAGGCAGG TCCGTGAAGCTGTGTTGTCCTGGAGTGACTGCCGGGGACCCAGTGTCCTGGTTTCGGGATGGGGAGCCAAAGCTGCTCCAGGGACCTGACTCTGGGCTAG GGCATGAACTGGTCCTGGCCCAGGCAGACAGCACTGATGAGGGCACCTACATCTGCCAGACCCTGGATGGTGCACTTGGGGGCACAGTGACCCTGCAGCT GGGCTACCCTCCAGCCCGCCCTGTTGTCTCCTGCCAAGCAGCCGACTATGAGAACTTCTCTTGCACTTGGAGTCCCAGCCAGATCAGCGGTTTACCCACC CGCTACCTCACCTCCTACAGGAAGAAGACAGTCCTAGGAGCTGATAGCCAGAGGAGGAGTCCATCCACAGGGCCCTGGCCATGCCCACAGGATCCCCTAG GGGCTGCCCGCTGTGTTGTCCACGGGGCTGAGTTCTGGAGCCAGTACCGGATTAATGTGACTGAGGTGAACCCACTGGGTGCCAGCACACGCCTGCTGGA TGTGAGCTTGCAGAGCATCTTGCGCCCTGACCCACCCCAGGGCCTGCGGGTAGAGTCAGTACCAGGTTACCCCCGACGCCTGCGAGCCAGCTGGACATAC CCTGCCTCCTGGCCGTGCCAGCCCCACTTCCTGCTCAAGTTCCGTTTGCAGTACCGTCCGGCGCAGCATCCAGCCTGGTCCACGGTGGAGCCAGCTGGAC TGGAGGAGGTGATCACAGATGCTGTGGCTGGGCTGCCCCATGCTGTACGAGTCAGTGCCCGGGACTTTCTAGATGCTGGCACCTGGAGCACCTGGAGCCC GGAGGCCTGGGGAACTCCGAGCACTGGGACCATACCAAAGGAGATACCAGCATGGGGCCAGCTACACACGCAGCCAGAGGTGGAGCCTCAGGTGGACAGC CCTGCTCCTCCAAGGCCCTCCCTCCAACCACACCCTCGGCTACTTGATCACAGGGACTCTGTGGAGCAGGTAGCTGTGCTGGCGTCTTTGGGAATCCTTT CTTTCCTGGGACTGGTGGCTGGGGCCCTGGCACTGGGGCTCTGGCTGAGGCTGAGACGGGGTGGGAAGGATGGATCCCCAAAGCCTGGGTTCTTGGCCTC AGTGATTCCAGTGGACAGGCGTCCAGGAGCTCCAAACCTGTAGAGGACCCAGGAGGGCTTCGGCAGATTCCACCTATAATTCTGTCTTGCTGGTGTGGAT AGAAACCAGGCAGGACAGTAGATCCCTATGGTTGGATCTCAGCTGGAAGTTCTGTTTGGAGCCCATTTCTGTGAGACCCTGTATTTCAAATTTGCAGCTG AAAGGTGCTTGTACCTCTGATTTCACCCCAGAGTTGGAGTTCTGCTCAAGGAACGTGTGTAATGTGTACATCTGTGTCCATGTGTGACCATGTGTCTGTG AGGCAGGGAACATGTATTCTCTGCATGCATGTATGTAGGTGCCTGGGGAGTGTGTGTGGGTCCTTGGCTCTTGGCCTTTCCCCTTGCAGGGGTTGTGCAG GTGTGAATAAAGAGAATAAGGAAGTTCTTGGAGATTATACTCAGAAATTATTATCCAATGCTGCTTTATTATTTGGCTATTGGGGGCTTCAGCCCATTTT CCTTAGCATCCCAAAATTCAGCTTGGGCAGAGTCCCATGGAGCTTTCTCTCTTGGTGCTCAAACCACTGTGACAGGCTGGGGTTCTGGGGGTGGATGCAG ATGCTGCGTTGAGCCAGGTGAAG

GALT.F42 (forward primer)
GALT.R41 (reverse primer)
Intron retention

Frame 0

ALAVSVASFPDLGEASSLPRALGGREGGRRQEQ GGGLARGGRAPGLRGGGAGKGKEGMALGSQN*VE *ETGAVAGERKS*RLWTLCCWDHRDEQQLLRAE QGPGGRGYSPGVCLLPLPPGLGPPRGPVWAARQ VREAVLSWSDCRGPSVLVSGWGAKAAPGT*LWAR A*TGPGPGRQH**GHLHLPDPGWCTWGHSDPAA GLPSSPPCCLLPSSRL*ELLLHLESQPDQRFTH PLPHLLQEEDSPRS**PEEESIHRALAMPTGSPR GCPLCCPRG*VLEPVPD*CD*GEPTGCQHTPAG CELAEHLAP*PTPGPAGRVSTRLPPTPASQLDI PCLLAVPAPLPAQVPFAVPSGAASSLVHGGASWT GGGDHRCCGWAAPCCTSQCPGLSRCWHLEHLEP GGLGNSEHWDHTKGDTSMGPATHAARGGASGGQ PCSSKALPPTTPSAT*SQGLCGAGSCAGVFGNPF FPGTGGWGPGTGALAEAETGWEGWIPKAWVLGL SDSSGQASRSSKPVEDPGGLRQIPPIILSCWCG *KPGRTVDPYGWISAGSSVWSPFL*DPVFQICS* KVLVPLISPQSWSSAQGTCVMCTSVSMCDHVSV RQGTCILCMHVCRCLGSVCGSLALGLSPCRGCA GVNKENKEVLGDYTQKLLSNAALLFGYWGLQPIF LSIPKFSLGRVPWSFLSWCSNHCDRLGFWGWMQ MLR*AR*

Frame 1

PWLCLWLPFQTWGRPLPFPGPWVGGKEGGGRSR EGAWLGAAELQGLEGAGQGRGRRGWLWGPRTEW SRRRGL*LVRGSPRGYGHSAAGITEMSSSCSGLS RVLVAVATALVSASSPCPQAWGPPGVQYGQPGR SVKLCCPGVTAGDPVSWFRDGEPKLLQGPDSGL GHELVLAQADSTDEGTYICQTLDGALGGTVTLQL GYPPARPVVSCQAADYENFSCTWSPSQISGLPT RYLTSYRKKTVLGADSQRRSPSTGPWPCPQDPL GAARCVVHGAEFWSQYRINVTEVNPLGASTRLLD VSLQSILRPDPPQGLRVESVPGYPRRLRASWTY PASWPCQPHFLLKFRLQYRPAQHPAWSTVEPAG LEEVITDAVAGLPHAVRVSARDFLDAGTWSTWSP EAWGTPSTGTIPKEIPAWGQLHTQPEVEPQVDS PAPPRPSLQPHPRLLDHRDSVEQVAVLASLGIL SFLGLVAGALALGLWLRLRRGGKDGSPKPGFLAS VIPVDRRPGAPNL*RTQEGFGRFHL*FCLAGVD RNQAGQ*IPMVGSQLEVLFGAHFCETLYFKFAA ERCLYL*FHPRVGVLLKERV*CVHLCPCVTMCL* GREHVFSACMYVGAWGVCVGPWLLAFPLAGVVQ V*IKRIRKFLEIILRNYYPMLLYYLAIGGFSPF SLASQNSAWAESHGAFSLGAQTTVTGWGSGGGCR CCVEPGE

Frame 2

LGCVCGFLSRLGGGLFPSQGLGWEGRREEAGAG RGPG*GRQSSRA*RGRGREGEGGDGSGVPELSG VGDGGCSW*EEVLEAMDTLLLGSPR*AAAAQG* AGSWWPWLQPWCLPPPPAPRPGAPQGSSMGSQAG P*SCVVLE*LPGTQCPGFGMGSQSCSRDLTLG* GMNWSWPRQTALMRAPTSARPWMVHLGAQ*PCS WATLQPALLSPAKQPTMRTSLALGVPARSAVYPP ATSPPTGRRQS*ELIARGGVHPQGPGHAHRIP* GLPAVLSTGLSSGASTGLM*LR*THWVPAHACW M*ACRASCALTHPRACG*SQYQVTPDACEPAGHT LPPGRASPTSCSSSVCSTVRRSIQPGPRWSQLD WRR*SQMLWLGCPMLYESVPGTF*MLAPGAPGA RRPGELRALGPYQRRYQHGASYTRSQRWSLRWTA LLLQGPPSNHTLGYLITGTLWSR*LCWRLWESF LSWDWWLGPWHWGSG*G*DGVGRMDPQSLGSWP Q*FQWTGVQELQTCRGPRRASADSTYNSVLLVWI ETRQDSRSLWLDLSWKFCLEPISVRPCISNLQL KGACTSDFTPELEFCSRNVCNVYICVHV*PCVC EAGNMYSLHACM*VPGECVWVLGSWPFPLQGLCR CE*RE*GSSWRLYSEIIIQCCFIIWLLGASAHF P*HPKIQLGQSPMELSLLVLKPL*QAGVLGVDA DAALSQVK

Sequence GALT.dAug05

GCCTTGGCTGTGTCTGTGGCTTCCTTTCCAGACTTGGGGGAGGCCTCTTCCCTTCCCAGGGCCTTGGGTGGGAGGGAAGGAGGGAGGAGGCAGGAGCAGG GAGGGGGCCTGGCTAGGGGCGGCAGAGCTCCAGGGCTTAGAGGGGGCGGGGCAGGGAAGGGGAAGGAGGGGATGGCTCTGGGGTCCCAGAACTGAGTGGA GTAGGAGACGGGGGCTGTAGCTGGTGAGAGGAAGTCCTAGAGGCTATGGACACTCTGCTGCTGGGATCACCGAGATGAGCAGCAGCTGCTCAGGGCTGAG CAGGGTCCTGGTGGCCGTGGCTACAGCCCTGGTGTCTGCCTCCTCCCCCTGCCCCCAGGCCTGGGGCCCCCCAGGGGTCCAGTATGGGCAGCCAGGCAGG TCCGTGAAGCTGTGTTGTCCTGGAGTGACTGCCGGGGACCCAGTGTCCTGGTTTCGGGATGGGGAGCCAAAGCTGCTCCAGGGACCTGACTCTGGGCTAG GGCATGAACTGGTCCTGGCCCAGGCAGACAGCACTGATGAGGGCACCTACATCTGCCAGACCCTGGATGGTGCACTTGGGGGCACAGTGACCCTGCAGCT GGGCTACCCTCCAGCCCGCCCTGTTGTCTCCTGCCAAGCAGCCGACTATGAGAACTTCTCTTGCACTTGGAGTCCCAGCCAGATCAGCGGTTTACCCACC CGCTACCTCACCTCCTACAGGAAGAAGACAGTCCTAGGAGCTGATAGCCAGAGGAGGAGTCCATCCACAGGGCCCTGGCCATGCCCACAGGATCCCCTAG GGGCTGCCCGCTGTGTTGTCCACGGGGCTGAGTTCTGGAGCCAGTACCGGATTAATGTGACTGAGGTGAACCCACTGGGTGCCAGCACACGCCTGCTGGA TGTGAGCTTGCAGAGCATCTTGCGCCCTGACCCACCCCAGGGCCTGCGGGTAGAGTCAGTACCAGGTTACCCCCGACGCCTGCGAGCCAGCTGGACATAC CCTGCCTCCTGGCCGTGCCAGCCCCACTTCCTGCTCAAGTTCCGTTTGCAGTACCGTCCGGCGCAGCATCCAGCCTGGTCCACGGTGGAGCCAGCTGGAC TGGAGGAGGTGATCACAGATGCTGTGGCTGGGCTGCCCCATGCTGTACGAGTCAGTGCCCGGGACTTTCTAGATGCTGGCACCTGGAGCACCTGGAGCCC GGAGGCCTGGGGAACTCCGAGCACTGGGACCATACCAAAGGAGATACCAGCATGGGGCCAGCTACACACGCAGCCAGAGGTGGAGCCTCAGGTGGACAGC CCTGCTCCTCCAAGGCCCTCCCTCCAACCACACCCTCGGCTACTTGATCACAGGGACTCTGTGGAGCAGGTAGCTGTGCTGGCGTCTTTGGGAATCCTTT CTTTCCTGGGACTGGTGGCTGGGGCCCTGGCACTGGGGCTCTGGTAAGTGACTGCCATTGGTCCCTCAGCCTCTGATCCTCACACATGCTCTGATGCCCA TAGACCACATTCATCTCCACCCTTCATGACTGCCCGCTGAACCTGTCTGATTCTGGAACTACCTCCCCATACCTCCATCCCCCATGCCCCACTTGATTTT AACTGATTCCTCTCCTGACCCTTTACTAATAAACCCTTTGGCGGAGACTGAGATAACCCACATTGTTGGAGAGACAGCTGCCTTTCTATGCCCCAGGCTG AGGCTGAGACGGGGTGGGAAGGATGGATCCCCAAAGCCTGGGTTCTTGGCCTCAGTGATTCCAGTGGACAGGCGTCCAGGTGAGTAGGACATCCAGAAGA TTTGGACTTGGAGATGTTTGCCCCTATTTTGAGTGTCCAGATTAAGAGCTGGCTGCCCTAGTCATTTTAAAACATGCTGGGAATCCAAGTTGGGTCTCCT CATTTTAATGATGTCTAGGCTGAGGGCTGGGCCTTTCATTCTTGAGTCCCTGGGCTCAGAAGTGGGTCTCTTTCCCTCCTCTCAGGGTACTGAGGAAGGA CCCCAGGGAGCTCCAAACCTGTAGAGGACCCAGGAGGGCTTCGGCAGATTCCACCTATAATTCTGTCTTGCTGGTGTGGATAGAAACCAGGCAGGACAGT AGATCCCTATGGTTGGATCTCAGCTGGAAGTTCTGTTTGGAGCCCATTTCTGTGAGACCCTGTATTTCAAATTTGCAGCTGAAAGGTGCTTGTACCTCTG ATTTCACCCCAGAGTTGGAGTTCTGCTCAAGGAACGTGTGTAATGTGTACATCTGTGTCCATGTGTGACCATGTGTCTGTGAGGCAGGGAACATGTATTC TCTGCATGCATGTATGTAGGTGCCTGGGGAGTGTGTGTGGGTCCTTGGCTCTTGGCCTTTCCCCTTGCAGGGGTTGTGCAGGTGTGAATAAAGAGAATAA GGAAGTTCTTGGAGATTATACTCAGAAATTATTATCCAATGCTGCTTTATTATTTGGCTATTGGGGGCTTCAGCCCATTTTCCTTAGCATCCCAAAATTC AGCTTGGGCAGAGTCCCATGGAGCTTTCTCTCTTGGTGCTCAAACCACTGTGACAGGCTGGGGTTCTGGGGGTGGATGCAGATGCTGCGTTGAGCCAGGT GAAG

GALT.F42 (forward primer)
GALT.R41 (reverse primer)
Intron retention

Frame 0

ALAVSVASFPDLGEASSLPRALGGREGGRRQEQ GGGLARGGRAPGLRGGGAGKGKEGMALGSQN*VE *ETGAVAGERKS*RLWTLCCWDHRDEQQLLRAE QGPGGRGYSPGVCLLPLPPGLGPPRGPVWAARQ VREAVLSWSDCRGPSVLVSGWGAKAAPGT*LWAR A*TGPGPGRQH**GHLHLPDPGWCTWGHSDPAA GLPSSPPCCLLPSSRL*ELLLHLESQPDQRFTH PLPHLLQEEDSPRS**PEEESIHRALAMPTGSPR GCPLCCPRG*VLEPVPD*CD*GEPTGCQHTPAG CELAEHLAP*PTPGPAGRVSTRLPPTPASQLDI PCLLAVPAPLPAQVPFAVPSGAASSLVHGGASWT GGGDHRCCGWAAPCCTSQCPGLSRCWHLEHLEP GGLGNSEHWDHTKGDTSMGPATHAARGGASGGQ PCSSKALPPTTPSAT*SQGLCGAGSCAGVFGNPF FPGTGGWGPGTGALVSDCHWSLSL*SSHML*CP *TTFISTLHDCPLNLSDSGTTSPYLHPPCPT*F *LIPLLTLY**TLWRRLR*PTLLERQLPFYAPG* G*DGVGRMDPQSLGSWPQ*FQWTGVQVSRTSRR FGLGDVCPYFECPD*ELAALVILKHAGNPSWVS SF**CLG*GLGLSFLSPWAQKWVSFPPLRVLRKD PRELQTCRGPRRASADSTYNSVLLVWIETRQDS RSLWLDLSWKFCLEPISVRPCISNLQLKGACTS DFTPELEFCSRNVCNVYICVHV*PCVCEAGNMYS LHACM*VPGECVWVLGSWPFPLQGLCRCE*RE* GSSWRLYSEIIIQCCFIIWLLGASAHFP*HPKI QLGQSPMELSLLVLKPL*QAGVLGVDADAALSQV K

Frame 1

PWLCLWLPFQTWGRPLPFPGPWVGGKEGGGRSR EGAWLGAAELQGLEGAGQGRGRRGWLWGPRTEW SRRRGL*LVRGSPRGYGHSAAGITEMSSSCSGLS RVLVAVATALVSASSPCPQAWGPPGVQYGQPGR SVKLCCPGVTAGDPVSWFRDGEPKLLQGPDSGL GHELVLAQADSTDEGTYICQTLDGALGGTVTLQL GYPPARPVVSCQAADYENFSCTWSPSQISGLPT RYLTSYRKKTVLGADSQRRSPSTGPWPCPQDPL GAARCVVHGAEFWSQYRINVTEVNPLGASTRLLD VSLQSILRPDPPQGLRVESVPGYPRRLRASWTY PASWPCQPHFLLKFRLQYRPAQHPAWSTVEPAG LEEVITDAVAGLPHAVRVSARDFLDAGTWSTWSP EAWGTPSTGTIPKEIPAWGQLHTQPEVEPQVDS PAPPRPSLQPHPRLLDHRDSVEQVAVLASLGIL SFLGLVAGALALGLW*VTAIGPSASDPHTCSDAH RPHSSPPFMTAR*TCLILELPPHTSIPHAPLDF N*FLS*PFTNKPFGGD*DNPHCWRDSCLSMPQA EAETGWEGWIPKAWVLGLSDSSGQASR*VGHPED LDLEMFAPILSVQIKSWLP*SF*NMLGIQVGSP HFNDV*AEGWAFHS*VPGLRSGSLSLLSGY*GR TPGSSKPVEDPGGLRQIPPIILSCWCG*KPGRTV DPYGWISAGSSVWSPFL*DPVFQICS*KVLVPL ISPQSWSSAQGTCVMCTSVSMCDHVSVRQGTCI LCMHVCRCLGSVCGSLALGLSPCRGCAGVNKENK EVLGDYTQKLLSNAALLFGYWGLQPIFLSIPKF SLGRVPWSFLSWCSNHCDRLGFWGWMQMLR*AR *

Frame 2

LGCVCGFLSRLGGGLFPSQGLGWEGRREEAGAG RGPG*GRQSSRA*RGRGREGEGGDGSGVPELSG VGDGGCSW*EEVLEAMDTLLLGSPR*AAAAQG* AGSWWPWLQPWCLPPPPAPRPGAPQGSSMGSQAG P*SCVVLE*LPGTQCPGFGMGSQSCSRDLTLG* GMNWSWPRQTALMRAPTSARPWMVHLGAQ*PCS WATLQPALLSPAKQPTMRTSLALGVPARSAVYPP ATSPPTGRRQS*ELIARGGVHPQGPGHAHRIP* GLPAVLSTGLSSGASTGLM*LR*THWVPAHACW M*ACRASCALTHPRACG*SQYQVTPDACEPAGHT LPPGRASPTSCSSSVCSTVRRSIQPGPRWSQLD WRR*SQMLWLGCPMLYESVPGTF*MLAPGAPGA RRPGELRALGPYQRRYQHGASYTRSQRWSLRWTA LLLQGPPSNHTLGYLITGTLWSR*LCWRLWESF LSWDWWLGPWHWGSGK*LPLVPQPLILTHALMP IDHIHLHPS*LPAEPV*FWNYLPIPPSPMPHLIL TDSSPDPLLINPLAETEITHIVGETAAFLCPRL RLRRGGKDGSPKPGFLASVIPVDRRPGE*DIQK IWTWRCLPLF*VSRLRAGCPSHFKTCWESKLGLL ILMMSRLRAGPFILESLGSEVGLFPSSQGTEEG PQGAPNL*RTQEGFGRFHL*FCLAGVDRNQAGQ *IPMVGSQLEVLFGAHFCETLYFKFAAERCLYL* FHPRVGVLLKERV*CVHLCPCVTMCL*GREHVF SACMYVGAWGVCVGPWLLAFPLAGVVQV*IKRI RKFLEIILRNYYPMLLYYLAIGGFSPFSLASQNS AWAESHGAFSLGAQTTVTGWGSGGGCRCCVEPG E

Sequence GALT.gAug05

GCCTTGGCTGTGTCTGTGGCTTCCTTTCCAGACTTGGGGGAGGCCTCTTCCCTTCCCAGGGCCTTGGGTGGGAGGGAAGGAGGGAGGAGGCAGGAGCAGG GAGGGGGCCTGGCTAGGGGCGGCAGAGCTCCAGGGCTTAGAGGGGGCGGGGCAGGGAAGGGGAAGGAGGGGATGGCTCTGGGGTCCCAGAACTGAGTGGA GTAGGAGACGGGGGCTGTAGCTGGTGAGAGGAAGTCCTAGAGGCTATGGACACTCTGCTGCTGGGATCACCGAGATGAGCAGCAGCTGCTCAGGGCTGAG CAGGGTCCTGGTGGCCGTGGCTACAGCCCTGGTGTCTGCCTCCTCCCCCTGCCCCCAGGCCTGGGGCCCCCCAGGGGTCCAGTATGGGCAGCCAGGCAGG TCCGTGAAGCTGTGTTGTCCTGGAGTGACTGCCGGGGACCCAGTGTCCTGGTTTCGGGATGGGGAGCCAAAGCTGCTCCAGGGACCTGACTCTGGGCTAG GGCATGAACTGGTCCTGGCCCAGGCAGACAGCACTGATGAGGGCACCTACATCTGCCAGACCCTGGATGGTGCACTTGGGGGCACAGTGACCCTGCAGCT GGGCTACCCTCCAGCCCGCCCTGTTGTCTCCTGCCAAGCAGCCGACTATGAGAACTTCTCTTGCACTTGGAGTCCCAGCCAGATCAGCGGTTTACCCACC CGCTACCTCACCTCCTACAGATGGCCCCCTCCCCACCAGGAAGAAGACAGTCCTAGGAGCTGATAGCCAGAGGAGGAGTCCATCCACAGGGCCCTGGCCA TGCCCACAGGATCCCCTAGGGGCTGCCCGCTGTGTTGTCCACGGGGCTGAGTTCTGGAGCCAGTACCGGATTAATGTGACTGAGGTGAACCCACTGGGTG CCAGCACACGCCTGCTGGATGTGAGCTTGCAGAGCATCTTGCGCCCTGACCCACCCCAGGGCCTGCGGGTAGAGTCAGTACCAGGTTACCCCCGACGCCT GCGAGCCAGCTGGACATACCCTGCCTCCTGGCCGTGCCAGCCCCACTTCCTGCTCAAGTTCCGTTTGCAGTACCGTCCGGCGCAGCATCCAGCCTGGTCC ACGGTGGAGCCAGCTGGACTGGAGGAGGTGATCACAGATGCTGTGGCTGGGCTGCCCCATGCTGTACGAGTCAGTGCCCGGGACTTTCTAGATGCTGGCA CCTGGAGCACCTGGAGCCCGGAGGCCTGGGGAACTCCGAGCACTGGGACCATACCAAAGGAGATACCAGCATGGGGCCAGCTACACACGCAGCCAGAGGT GGAGCCTCAGGTGGACAGCCCTGCTCCTCCAAGGCCCTCCCTCCAACCACACCCTCGGCTACTTGATCACAGGGACTCTGTGGAGCAGGTAGCTGTGCTG GCGTCTTTGGGAATCCTTTCTTTCCTGGGACTGGTGGCTGGGGCCCTGGCACTGGGGCTCTGGCTGAGGCTGAGACGGGGTGGGAAGGATGGATCCCCAA AGCCTGGGTTCTTGGCCTCAGTGATTCCAGTGGACAGGCGTCCAGGAGCTCCAAACCTGTAGAGGACCCAGGAGGGCTTCGGCAGATTCCACCTATAATT CTGTCTTGCTGGTGTGGATAGAAACCAGGCAGGACAGTAGATCCCTATGGTTGGATCTCAGCTGGAAGTTCTGTTTGGAGCCCATTTCTGTGAGACCCTG TATTTCAAATTTGCAGCTGAAAGGTGCTTGTACCTCTGATTTCACCCCAGAGTTGGAGTTCTGCTCAAGGAACGTGTGTAATGTGTACATCTGTGTCCAT GTGTGACCATGTGTCTGTGAGGCAGGGAACATGTATTCTCTGCATGCATGTATGTAGGTGCCTGGGGAGTGTGTGTGGGTCCTTGGCTCTTGGCCTTTCC CCTTGCAGGGGTTGTGCAGGTGTGAATAAAGAGAATAAGGAAGTTCTTGGAGATTATACTCAGAAATTATTATCCAATGCTGCTTTATTATTTGGCTATT GGGGGCTTCAGCCCATTTTCCTTAGCATCCCAAAATTCAGCTTGGGCAGAGTCCCATGGAGCTTTCTCTCTTGGTGCTCAAACCACTGTGACAGGCTGGG GTTCTGGGGGTGGATGCAGATGCTGCGTTGAGCCAGGTGAAG

GALT.F42 (forward primer)
GALT.R41 (reverse primer)
Intron retention

Frame 0

ALAVSVASFPDLGEASSLPRALGGREGGRRQEQ GGGLARGGRAPGLRGGGAGKGKEGMALGSQN*VE *ETGAVAGERKS*RLWTLCCWDHRDEQQLLRAE QGPGGRGYSPGVCLLPLPPGLGPPRGPVWAARQ VREAVLSWSDCRGPSVLVSGWGAKAAPGT*LWAR A*TGPGPGRQH**GHLHLPDPGWCTWGHSDPAA GLPSSPPCCLLPSSRL*ELLLHLESQPDQRFTH PLPHLLQMAPSPPGRRQS*ELIARGGVHPQGPGH AHRIP*GLPAVLSTGLSSGASTGLM*LR*THWV PAHACWM*ACRASCALTHPRACG*SQYQVTPDA CEPAGHTLPPGRASPTSCSSSVCSTVRRSIQPGP RWSQLDWRR*SQMLWLGCPMLYESVPGTF*MLA PGAPGARRPGELRALGPYQRRYQHGASYTRSQR WSLRWTALLLQGPPSNHTLGYLITGTLWSR*LCW RLWESFLSWDWWLGPWHWGSG*G*DGVGRMDPQ SLGSWPQ*FQWTGVQELQTCRGPRRASADSTYN SVLLVWIETRQDSRSLWLDLSWKFCLEPISVRPC ISNLQLKGACTSDFTPELEFCSRNVCNVYICVH V*PCVCEAGNMYSLHACM*VPGECVWVLGSWPF PLQGLCRCE*RE*GSSWRLYSEIIIQCCFIIWLL GASAHFP*HPKIQLGQSPMELSLLVLKPL*QAG VLGVDADAALSQVK

Frame 1

PWLCLWLPFQTWGRPLPFPGPWVGGKEGGGRSR EGAWLGAAELQGLEGAGQGRGRRGWLWGPRTEW SRRRGL*LVRGSPRGYGHSAAGITEMSSSCSGLS RVLVAVATALVSASSPCPQAWGPPGVQYGQPGR SVKLCCPGVTAGDPVSWFRDGEPKLLQGPDSGL GHELVLAQADSTDEGTYICQTLDGALGGTVTLQL GYPPARPVVSCQAADYENFSCTWSPSQISGLPT RYLTSYRWPPPHQEEDSPRS**PEEESIHRALA MPTGSPRGCPLCCPRG*VLEPVPD*CD*GEPTGC QHTPAGCELAEHLAP*PTPGPAGRVSTRLPPTP ASQLDIPCLLAVPAPLPAQVPFAVPSGAASSLV HGGASWTGGGDHRCCGWAAPCCTSQCPGLSRCWH LEHLEPGGLGNSEHWDHTKGDTSMGPATHAARG GASGGQPCSSKALPPTTPSAT*SQGLCGAGSCA GVFGNPFFPGTGGWGPGTGALAEAETGWEGWIPK AWVLGLSDSSGQASRSSKPVEDPGGLRQIPPII LSCWCG*KPGRTVDPYGWISAGSSVWSPFL*DP VFQICS*KVLVPLISPQSWSSAQGTCVMCTSVSM CDHVSVRQGTCILCMHVCRCLGSVCGSLALGLS PCRGCAGVNKENKEVLGDYTQKLLSNAALLFGY WGLQPIFLSIPKFSLGRVPWSFLSWCSNHCDRLG FWGWMQMLR*AR*

Frame 2

LGCVCGFLSRLGGGLFPSQGLGWEGRREEAGAG RGPG*GRQSSRA*RGRGREGEGGDGSGVPELSG VGDGGCSW*EEVLEAMDTLLLGSPR*AAAAQG* AGSWWPWLQPWCLPPPPAPRPGAPQGSSMGSQAG P*SCVVLE*LPGTQCPGFGMGSQSCSRDLTLG* GMNWSWPRQTALMRAPTSARPWMVHLGAQ*PCS WATLQPALLSPAKQPTMRTSLALGVPARSAVYPP ATSPPTDGPLPTRKKTVLGADSQRRSPSTGPWP CPQDPLGAARCVVHGAEFWSQYRINVTEVNPLG ASTRLLDVSLQSILRPDPPQGLRVESVPGYPRRL RASWTYPASWPCQPHFLLKFRLQYRPAQHPAWS TVEPAGLEEVITDAVAGLPHAVRVSARDFLDAG TWSTWSPEAWGTPSTGTIPKEIPAWGQLHTQPEV EPQVDSPAPPRPSLQPHPRLLDHRDSVEQVAVL ASLGILSFLGLVAGALALGLWLRLRRGGKDGSP KPGFLASVIPVDRRPGAPNL*RTQEGFGRFHL*F CLAGVDRNQAGQ*IPMVGSQLEVLFGAHFCETL YFKFAAERCLYL*FHPRVGVLLKERV*CVHLCP CVTMCL*GREHVFSACMYVGAWGVCVGPWLLAFP LAGVVQV*IKRIRKFLEIILRNYYPMLLYYLAI GGFSPFSLASQNSAWAESHGAFSLGAQTTVTGW GSGGGCRCCVEPGE

Sequence GALT.voAug05

GALT.F42 (forward primer)
GALT.R41 (reverse primer)
Intron retention

Frame 0

ALAVSVASFPDLGEASSLPRALGGREGGRRQEQ GGGLARGGRAPGLRGGGAGKGKEGMALGSQN*VE *ETGAVAGERKS*RLWTLCCWDHRDEQQLLRAE QGPGGRGYSPGVCLLPLPPGLGPPR*EEPCSTT SQL*LVTCHPHCGPFPCLTCDTTPAPVIQPGSSL YPVL*PLSPDYNMML*SSEPPTTQVLTVCFLSV ILTKWGGG*RKSLTSEVPSTGVQYGQPGRSVKL CCPGVTAGDPVSWFRDGEPKLLQGPDSGLGHELV LAQADSTDEGTYICQTLDGALGGTVTLQLGCEL GRVALMTHRDPEGVWDLSKPSGTRRT*GLLSLQ VYPLPLDPPARPVVSCQAADYENFSCTWSPSQIS GLPT

Frame 1

PWLCLWLPFQTWGRPLPFPGPWVGGKEGGGRSR EGAWLGAAELQGLEGAGQGRGRRGWLWGPRTEW SRRRGL*LVRGSPRGYGHSAAGITEMSSSCSGLS RVLVAVATALVSASSPCPQAWGPPGEKNPALQP PNSDL*LATLTVAPSHA*LVTPPLLLSSNLGPL STLFSDLCLQIIT*CFDPVSPPPPRF*QSASYLS FSQSGEGGKGRALPQKCPPQGSSMGSQAGP*SC VVLE*LPGTQCPGFGMGSQSCSRDLTLG*GMNW SWPRQTALMRAPTSARPWMVHLGAQ*PCSWAVSW GGWH**HIGILREYGT*VSPLGPGGPEDCSVSR CTLCL*TLQPALLSPAKQPTMRTSLALGVPARS AVYPP

Frame 2

LGCVCGFLSRLGGGLFPSQGLGWEGRREEAGAG RGPG*GRQSSRA*RGRGREGEGGDGSGVPELSG VGDGGCSW*EEVLEAMDTLLLGSPR*AAAAQG* AGSWWPWLQPWCLPPPPAPRPGAPQVRRTLLYNL PTLTCDLPPSLWPLPMPNL*HHPCSCHPTWVLS LPCSLTSVSRL*HDALIQ*APHHPGFNSLLLIC HSHKVGRGVKEEPYLRSALHRGPVWAARQVREAV LSWSDCRGPSVLVSGWGAKAAPGT*LWARA*TG PGPGRQH**GHLHLPDPGWCTWGHSDPAAGL*V GEGGTDDT*GS*GSMGPK*ALWDQEDLRTAQSPG VPSASRPSSPPCCLLPSSRL*ELLLHLESQPDQ RFTHP