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Sequences

Sequence HLA-DQA1.cAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGTTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACG GTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAA ATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACC AATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTGTGG TCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTA CCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCA GCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAGGCC TGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTA TGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTT AGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCC CTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGG ATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCT TGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATA CTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACC TCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGA AACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACA GTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCT CATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTA ATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTG AAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATAT TTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAA GCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTT TTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATAT ATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGG AGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCA GGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAAT GTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCT ATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGG GAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCT TGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCC ACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTT CTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTA TGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGA ACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGT CATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTA TTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTA CTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAG TGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATA GGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTC CTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCA GCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTAC CACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAA TTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTAC TAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTA TGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACT TTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F1 (forward primer)
HLA-DQA1.R1 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGVVADHVASCGVNLYQFY GPSGQYTHEFDGDEQFYVDLERKETAWRWPEFSK FGGFDPQGALRNMAVAKHNLNIMIKRYNSTAAT NEVPEVTVFSKSPVTLGQPNTLICLVDNIFPPV VNITWLSNGQSVTEGVSETSFLSKSDHSFFKISY LTFLPSADEIYDCKVEHWGLDQPLLKHWEPEIP APMSELTETVVCALGLSVGLMGIVVGTVFIIQG LRSVGASRHQGPL*IPSWKGRAD*NYG**LGYSY VSIQFNLISQIIFHVQ*HRSHQVQYSLIIC*FL S*H*YFLLPCVPTLGTATHPSIQATMKLMDTVC PWPRIVIAKILEPKNKSVLISMWLLKV*QRNKLG *RKFESQKYQK*KCILKTLNL*RMMTVEAFLFP CHLEIKIL*AGKEMEVRKTLE*DNNVGV*KGTN TYSSHRVSDNGKRDGSRSHRHI*EP*IEAQSASP PE*SCTR*PCSFPCATLA*RRQYSEYGYRMFLG NMPISSEIPSPFP*PLLTKESMSL*EKP*EEQH S*DNVAAAVGAVSSTGFAISEEKLSQRKWVSSDL MALNSYEIHEDISNHATCISEFKF*LEKKYKTL MQ*LIQYSLVQRTLNPNPGLSTLKLVF*TL*MC EVSNISGLTFLIHNVGVIILSLQSY**NLNNFDI *SMPYT*YMNT*ENIVVIFIINLFKRMDHII*K ALLFFSAS**FRRFKCRAMGEFLFI**LKDIFL FPE*ERLRLDW*ENS*H*KL*YLTLVFNSLRGNI FPPYGP*VFIQGISYTTDLYACLLWGRSEDSSR RK*FKTQTGNQ*AQEI*TSDDHENVHHRAQMIF RAIKLFCLIPQW*KMSLYICSNPQNI*HQELDLM *TMDFG***CANVSS*IIANVPLRWEMLLMGEA MHVWEQSVSGSSPQPFWQQGPVLWKTIFPQIVG GMGV*SGDETVKLFHLRSTSIS*ILIRNMQPRSL ACTVKNRVRTPMRI*CHH*SDGRRGSVGNAGSP TAWSSF*QATNWYWSMARGLGTSGIPLHLLLSF SVNLQPL*NKVYF*KGKEKVTNYDPKCVK*KF*Y EKQSH*NAQRAKKLLAIGFQIYFIYISMIREAR TQHFGVVCICVCVCVCVCVSVI*GVY*MA**PE SCRNLQMLPNSDSFLKQHCGKQI***QYFIKRMY SKNIYVMLK*QN*N*F*KIRVNSSFYQLKM*GL LSIGLQIYLPDLKSCNS*TII*AI*IISDYTHK V*LH*YLISHYLKFTKLVGHRGSCL*S*HFGGQ* GRIA*SQEFNSCLGNKARPCLYNTKIN*CMAAL L*SQLL*RLRQEECLSPGVQYFSGLRLCHCAHS SLGSRAGLHL*K*INK*NLQNFKNQMKYVRFVPT TFKLGT*RIKHFITNEALHAQTGT**VVDRC*Q FVLLLFSGVQLTNPTVNDITGMQPTRSRIWKLL LDKQLHFFSNNSREREKRSYTF*KAEEIYEPNLY EAIRKTDTDCIKELSNFSVVMRLLLCFLKSHYT LDFHNKIIMNE

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVELWLTTLPLVV*TCTSFT VPLASTPMNLMEMSSSTWTWRGRRLPGGGLSSA NLEVLTRRVH*ETWLWQNTT*TS*LNATTLPLLP MRFLRSQCFPSLP*HWVSPTPSFVLWTTSFLLW STSHG*AMGSQSQKVFLRPASSPRVIIPSSRSV TSPSSLLLMRFMTARWSTGAWTSLF*NTGSLRFQ PLCQSSQRLWSVPWGCLWASWALWWALSSSSKA CVQLVLPDTKGHCESHPGREGLTETMANNWGTL MFQSNLTSFPRSFFMSSNTEATKYSIA**YVDFL ADINISCFLVFPPLALPPTPQFRQQ*N*WIPSA LGPELL*QKF*NQKISLY*FQCGF*KYDREIS* DKGNLNLKNIKSKNVFSKL*IYEE**Q*KPSSSL VTLR*KFFRQEKKWKSEKH*NKTIMWVSEKEQI LIPHIGLVTMGKGTGVEATDISRSPE*RRSLPH LLNEAVLDNHVAFPVPLWHEGDSIVNMATGCFLE TCQYLQKSPALSPNPS*LRKA*AYERNPRKNNT VETM*QQQWVLCPPLDSPSPRRNSHRGNGSAAT SWL*TAMKSMRIFLTMLPASVSLNFNWRKNTKH* CNN*YSIVWCKEP*IQIQDSVL*S*YFKLYKCV KSLTFLALLFSSTM*E**YFPCRVIDKI*IILI FSQCLTHST*THKKTLWLYL*LIYLKEWIILYEK HFCFSQPLNDLGDSNVELWVNFFSYDN*RIFFF FQNERG*DWIGKRTLSTRSCNI*LWFSTL*EGI YSLLMAHKCLFKVYHIQQIFMPVYFGEEVKIVQG ENNLKHRLGISKHRKSEPVMIMKMSITEHR*FS GQ*NYSV*YHNGEKCHYTFAQIHRIYNTKS*T* CKLWTLGDNDVPM*VHKL*QMYHSDGRCC*WGRL CMCGSRVYLAAVPSLFGSRDQFYGRQFFHR*LG EWGYDLGMKL*NYSTSDQQALVRFS*GTCNLDL LHAQLKIGFALL*ESNATTDLTEGGAQLVMLAHP LRGPVSNRPPTGTGPWPGGWGPLGYLCTFCSVF L*TYNRFKIRSIFKKEKRR*PIMILNV*NKNFS MKNSHIKMHNVLRSF*Q*GFKYISYTFQ*FVRQE PSILELCAFVCVCVCVCVCL*YKGYTEWHNDQS HAEIYKCCPTQTPSSSSTVESKFNDSSILLKGC IQKIFT*C*NSRIKTNSKK*E*IVLFIS*KCRAY YQ*GYRSTCLI*NHAILKQLFELFELFQITHIK CDFINT*YHTI*NLQN*WVTEARACDLSTLEAN RGGLLEARSSTAAWATKQDLVSTTQK*INAWRHS CSPSYSRG*GKRSA*AQEFNTSVG*DCATVLTP A*AAEQDSISKSK*INKTYKILKIK*NMSGLYL PHLN*GLEELNILLQMKHFMHRLAHSK*SIGVNN LCYCYFLESN*QIPQ*MTSQGCNQQDPEYGNFY *INNSISSATIQERERREAIHFKRLKKYMNQIC MRQSEKLTPTVLRNYLILVW**DCCYVF*KVIIL *IFIIK*L*MK

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWSCG*PRCLLWCKLVPVLR SLWPVHP*I*WR*AVLRGPGEEGDCLAVA*VQQ IWRF*PAGCTEKHGCGKTQLEHHD*TLQLYRCY Q*GS*GHSVFQVSRDTGSAQHPHLSCGQHLSSCG QHHMAEQWAVSHRRCF*DQLPLQE*SFLLQDQL PHLPPFC**DL*LQGGALGPGPASSETLGA*DS SPYVRAHRDCGLCPGVVCGPHGHCGGHCLHHPRP AFSWCFQTPRAIVNPILEGKG*LKLWLIIGVLL CFNPI*PHFPDHFSCPVTQKPPSTV*PDNMLIS *LTLIFLASLCSHPWHCHPPLNSGNNEINGYRLP LAQNCYSKNFRTKK*VCTNFNVAFKSMTEK*VR IKEI*ISKISKVKMYSQNFKFMKNDDSRSLPLP LSP*DKNSLGRKRNGSQKNIRIRQ*CGCLKRNKY LFLT*G**QWEKGRE*KPQTYLGALNRGAVCLT S*MKLY*ITM*LSLCHSGMKETV**IWLQDVSW KHANIFRNPQPFPLTPPD*GKHELMRETLGRTTQ LRQCSSSSGCCVLHWIRHLRGETLTEEMGQQRP HGSKQL*NP*GYF*PCYLHQ*V*ILIGEKIQNI NAIIDTV*FGAKNPKSKSRTQYFEASILNFINV* SL*HFWPYFSHPQCRSNNTFLAELLIKFE*F*Y LVNALHIVHEHIRKHCGYIYN*FI*KNGSYYMK STFVFLSLLMI*EIQM*SYG*ISFHMIIEGYFSF SRMREAEIGLVRELLALEVVIFDFGFQLSKREY IPSLWPISVYSRYIIYNRSLCLFTLGKK*R*FK EKII*NTDWESVSTGNLNQ**S*KCPSQSTDDFQ GNKTILFDTTMVKNVIIHLLKSTEYITPRVRLN VNYGLWVIMMCQCKFINYSKCTTQMGDVANGGG YACVGAECIWQQSPAFLAAGTSFMEDNFSTDSWG NGGMIWG*NCETIPPQINKH*LDSHKEHAT*IS CMHS*K*GSHSYENLMPPLI*RKAGLSW*CWLT HCVVQFLTGHQLVLVHGPGVGDLWDTSAPSAQFF CEPTTALK*GLFLKRKREGNQL*S*MCKIKILV *KTVTLKCTTC*EAFSNRVSNIFHIHFNDS*GK NPAFWSCVHLCVCVCVCVCVCNIRGILNGIMTRV MQKSTNAAQLRLLPQAALWKANLMIAVFY*KDV FKKYLRNVKIAELKLILKNKSK*FFLSAKNVGP TINRVTDLPA*FKIMQFLNNYLSYLNYFRLHT*S VTSLILNITLFKIYKISGSQRLVLVILALWRPI GEDCLKPGVQQLPGQQSKTLSLQHKNKLMHGGT PVVPATLEAEARGVLEPRSSILQWAKIVPLCSLQ PRQQSRTPSLKVNK*IKLTKF*KSNEICQVCTY HI*TRDLKN*TFYYK*STSCTDWHIVSSR*VLT ICVIVIFWSPTNKSHSE*HHRDATNKIQNMETST R*TTPFLQQQFKREREEKLYILKG*RNI*TKFV *GNQKN*HRLY*GII*F*CGNEIAVMFSKKSLY FRFS**NNYE*

Sequence HLA-DQA1.bAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCT GAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCA TCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAAT GGACTTGCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGC TGCTATATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTG GGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCAT CTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGT AACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGC CACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCT GTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCA AAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAG AAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGA AGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTC TGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCT GACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTC GCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTA CCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAA TCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTA ATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGG TTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAG AGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCAC TAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAA CAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTG AACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTA TACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAA TTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTG GCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGA TCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCC ACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGC CCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAA GAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAA TAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAG CACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCT AAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTC TTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTA GTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAG CAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCC AGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAA AACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAG CACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAA TGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAG AGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATT TTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F1 (forward primer)
HLA-DQA1.R1 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVADHVASCGVNLY QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWEP EIPAPMSELTETVVCALGLSVGLMGIVVGTVFI IQGLRSVGASRHQGPL*IPSWKGRCIAIYRSRRM DLLNDLALFSGPIYHIPFLLQIFLLSPFLWDLS CYIPSELTNAFTFFP*PPDFFFLFSNVTYKDMP GVSHPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSI FHPGLTETMANNWGTLMFQSNLTSFPRSFFMSS NTEATKYSIA**YVDFLADINISCFLVFPPLAL PPTPQFRQQ*N*WIPSALGPELL*QKF*NQKISL Y*FQCGF*KYDREIS*DKGNLNLKNIKSKNVFS KL*IYEE**Q*KPSSSLVTLR*KFFRQEKKWKS EKH*NKTIMWVSEKEQILIPHIGLVTMGKGTGVE ATDISRSPE*RRSLPHLLNEAVLDNHVAFPVPL WHEGDSIVNMATGCFLETCQYLQKSPALSPNPS *LRKA*AYERNPRKNNTVETM*QQQWVLCPPLDS PSPRRNSHRGNGSAATSWL*TAMKSMRIFLTML PASVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQ IQDSVL*S*YFKLYKCVKSLTFLALLFSSTM*E* *YFPCRVIDKI*IILIFSQCLTHST*THKKTLW LYL*LIYLKEWIILYEKHFCFSQPLNDLGDSNV ELWVNFFSYDN*RIFFFFQNERG*DWIGKRTLST RSCNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQ QIFMPVYFGEEVKIVQGENNLKHRLGISKHRKS EPVMIMKMSITEHR*FSGQ*NYSV*YHNGEKCHY TFAQIHRIYNTKS*T*CKLWTLGDNDVPM*VHK L*QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLF GSRDQFYGRQFFHR*LGEWGYDLGMKL*NYSTSD QQALVRFS*GTCNLDLLHAQLKIGFALL*ESNA TTDLTEGGAQLVMLAHPLRGPVSNRPPTGTGPW PGGWGPLGYLCTFCSVFL*TYNRFKIRSIFKKEK RR*PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q *GFKYISYTFQ*FVRQEPSILELCAFVCVCVCV CVCL*YKGYTEWHNDQSHAEIYKCCPTQTPSSSS TVESKFNDSSILLKGCIQKIFT*C*NSRIKTNS KK*E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAI LKQLFELFELFQITHIKCDFINT*YHTI*NLQN* WVTEARACDLSTLEANRGGLLEARSSTAAWATK QDLVSTTQK*INAWRHSCSPSYSRG*GKRSA*A QEFNTSVG*DCATVLTPA*AAEQDSISKSK*INK TYKILKIK*NMSGLYLPHLN*GLEELNILLQMK HFMHRLAHSK*SIGVNNLCYCYFLESN*QIPQ* MTSQGCNQQDPEYGNFY*INNSISSATIQERERR EAIHFKRLKKYMNQICMRQSEKLTPTVLRNYLI LVW**DCCYVF*KVIIL*IFIIK*L*MK

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGSL RFQPLCQSSQRLWSVPWGCLWASWALWWALSSS SKACVQLVLPDTKGHCESHPGREGASPSTGAEE WTC*MT*HYSLARFIISLFSSKYFSSHLFSGT*A AISPQSSQMPLHSFPDLLIFFFFSQMLPTKTCL G*ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFP SFIQG*LKLWLIIGVLLCFNPI*PHFPDHFSCPV TQKPPSTV*PDNMLIS*LTLIFLASLCSHPWHC HPPLNSGNNEINGYRLPLAQNCYSKNFRTKK*V CTNFNVAFKSMTEK*VRIKEI*ISKISKVKMYSQ NFKFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQ KNIRIRQ*CGCLKRNKYLFLT*G**QWEKGRE* KPQTYLGALNRGAVCLTS*MKLY*ITM*LSLCHS GMKETV**IWLQDVSWKHANIFRNPQPFPLTPP D*GKHELMRETLGRTTQLRQCSSSSGCCVLHWI RHLRGETLTEEMGQQRPHGSKQL*NP*GYF*PCY LHQ*V*ILIGEKIQNINAIIDTV*FGAKNPKSK SRTQYFEASILNFINV*SL*HFWPYFSHPQCRS NNTFLAELLIKFE*F*YLVNALHIVHEHIRKHCG YIYN*FI*KNGSYYMKSTFVFLSLLMI*EIQM* SYG*ISFHMIIEGYFSFSRMREAEIGLVRELLA LEVVIFDFGFQLSKREYIPSLWPISVYSRYIIYN RSLCLFTLGKK*R*FKEKII*NTDWESVSTGNL NQ**S*KCPSQSTDDFQGNKTILFDTTMVKNVI IHLLKSTEYITPRVRLNVNYGLWVIMMCQCKFIN YSKCTTQMGDVANGGGYACVGAECIWQQSPAFL AAGTSFMEDNFSTDSWGNGGMIWG*NCETIPPQ INKH*LDSHKEHAT*ISCMHS*K*GSHSYENLMP PLI*RKAGLSW*CWLTHCVVQFLTGHQLVLVHG PGVGDLWDTSAPSAQFFCEPTTALK*GLFLKRK REGNQL*S*MCKIKILV*KTVTLKCTTC*EAFSN RVSNIFHIHFNDS*GKNPAFWSCVHLCVCVCVC VCVCNIRGILNGIMTRVMQKSTNAAQLRLLPQA ALWKANLMIAVFY*KDVFKKYLRNVKIAELKLIL KNKSK*FFLSAKNVGPTINRVTDLPA*FKIMQF LNNYLSYLNYFRLHT*SVTSLILNITLFKIYKI SGSQRLVLVILALWRPIGEDCLKPGVQQLPGQQS KTLSLQHKNKLMHGGTPVVPATLEAEARGVLEP RSSILQWAKIVPLCSLQPRQQSRTPSLKVNK*I KLTKF*KSNEICQVCTYHI*TRDLKN*TFYYK*S TSCTDWHIVSSR*VLTICVIVIFWSPTNKSHSE *HHRDATNKIQNMETSTR*TTPFLQQQFKRERE EKLYILKG*RNI*TKFV*GNQKN*HRLY*GII*F *CGNEIAVMFSKKSLYFRFS**NNYE*

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA* VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ DQLPHLPPFC**DL*LQGGALGPGPASSETLGA *DSSPYVRAHRDCGLCPGVVCGPHGHCGGHCLHH PRPAFSWCFQTPRAIVNPILEGKVHRHLQEQKN GLAK*PSTILWPDLSYPFSPPNISPLTFSLGLK LLYPLRAHKCLYILSLTS*FFFSFLKCYLQRHAW GKPPGYLIPQ*PPSKISKEAINSFYEIYVKFFH LSSRAD*NYG**LGYSYVSIQFNLISQIIFHVQ *HRSHQVQYSLIIC*FLS*H*YFLLPCVPTLGTA THPSIQATMKLMDTVCPWPRIVIAKILEPKNKS VLISMWLLKV*QRNKLG*RKFESQKYQK*KCIL KTLNL*RMMTVEAFLFPCHLEIKIL*AGKEMEVR KTLE*DNNVGV*KGTNTYSSHRVSDNGKRDGSR SHRHI*EP*IEAQSASPPE*SCTR*PCSFPCAT LA*RRQYSEYGYRMFLGNMPISSEIPSPFP*PLL TKESMSL*EKP*EEQHS*DNVAAAVGAVSSTGF AISEEKLSQRKWVSSDLMALNSYEIHEDISNHA TCISEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPN PGLSTLKLVF*TL*MCEVSNISGLTFLIHNVGV IILSLQSY**NLNNFDI*SMPYT*YMNT*ENIV VIFIINLFKRMDHII*KALLFFSAS**FRRFKCR AMGEFLFI**LKDIFLFPE*ERLRLDW*ENS*H *KL*YLTLVFNSLRGNIFPPYGP*VFIQGISYT TDLYACLLWGRSEDSSRRK*FKTQTGNQ*AQEI* TSDDHENVHHRAQMIFRAIKLFCLIPQW*KMSL YICSNPQNI*HQELDLM*TMDFG***CANVSS* IIANVPLRWEMLLMGEAMHVWEQSVSGSSPQPFW QQGPVLWKTIFPQIVGGMGV*SGDETVKLFHLR STSIS*ILIRNMQPRSLACTVKNRVRTPMRI*C HH*SDGRRGSVGNAGSPTAWSSF*QATNWYWSMA RGLGTSGIPLHLLLSFSVNLQPL*NKVYF*KGK EKVTNYDPKCVK*KF*YEKQSH*NAQRAKKLLA IGFQIYFIYISMIREARTQHFGVVCICVCVCVCV CVSVI*GVY*MA**PESCRNLQMLPNSDSFLKQ HCGKQI***QYFIKRMYSKNIYVMLK*QN*N*F *KIRVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS *TII*AI*IISDYTHKV*LH*YLISHYLKFTKL VGHRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNK ARPCLYNTKIN*CMAALL*SQLL*RLRQEECLSP GVQYFSGLRLCHCAHSSLGSRAGLHL*K*INK* NLQNFKNQMKYVRFVPTTFKLGT*RIKHFITNE ALHAQTGT**VVDRC*QFVLLLFSGVQLTNPTVN DITGMQPTRSRIWKLLLDKQLHFFSNNSREREK RSYTF*KAEEIYEPNLYEAIRKTDTDCIKELSN FSVVMRLLLCFLKSHYTLDFHNKIIMNE

Sequence HLA-DQA1.eAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGGATCCATCTCTGACTCCCTGCTCCTTTATAGAGATG GACAGTGGGTTTGTAAAACAAAAGTTGAAAAGTCAGATAGTTAAAAGGGGAAGTGAACTGGAAGGTACTTTAAACTTTCACAACTTTATTAACCGTGGCT GCTCCCATTATGATTTTGTTCGGCAGTGGAAGTTTCACCTGCTTCTCCAGAGCGCTTGGCGTCTTTGTTCCAAATTTCCTTTCTTCAACCTCACACCAGA GTGCCCTGGTCAGGCTTGGCTCATTCATTAGGCACAATGTGGGCAGTGCAGGGGACCCTCCATACTGTAAAGCCACATGAGAAGGTTTTAACTCCTTTTA AAATTAGAAAAATAATGGAATATTAGAGCCTAAGAAAATGTTTTAACTTTTAATTCAGCCTAGATTATATTGTCTTTATACCAATTCAGTCATAAAATAT AATTTTCCATATTTTTGTGGAGGAAGAGGCCCATACAAGCAAGAATGCTCGGGGCTCACATGTCAGAATGCAGCCTTGGTCATGACTGATCCTGGCCTTC GTATGGTTCTGCTACCTGTGTGCCTGCCCGTCTTCCCCAAAATCTATGTGGTCCTCGAATATAGCAACTGTCATTCAATACACATGTTTGAGCACACAGT GAGCTAAGTTTTAAGGATTCAAAGATGAAAAGTCATGCTGTCTTCCCTGCAGAGGGTGCTCAGATTAGTGATGGAAACAGTATGGGGTGCAGGAAAGCAG AAGGCCATTGCTGAGCAGGGCAGTGGACCCAGCAGAGGCTGAAACAATACAAGTGACTTGGTTCCAGGTGGGCCAGCAGGGTGACGTCCTCTAGCAAAAT TTGGCACCCAAGACAAGTACCAGAAGAAAAGAAAGACTGCATGTATTCCACATATATTCATGTTTGAACAAGGAGTCAAGATTTATGGCAAGGATAAGGA GGCTTTGTTGGTGACCTGTTAAGACCAACCAGGGCAGTCATGCTGGATAGGGAAGAAGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTT ACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAG CAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCT ACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTG TGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAG TTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATT CCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAG GCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAATGGACTT GCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGCTGCTAT ATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTGGGGTAA GCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCA TCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACA GAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCA CCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTA ATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTT TAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAAC ATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCAC AGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCAT GAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAA GGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATC TCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCA TCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGG ACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATA CTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATAT TTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTAT GGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAG TTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATC TTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAG TGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATT TGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAG CAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCA GGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACA AGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACT GATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGG GTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAG GTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGT TTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGT GTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGT GGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAA TAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAAC AATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGT CACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGAC CTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGT TCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTA CAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTC ATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACAT CACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGC TATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTG TGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F1 (forward primer)
HLA-DQA1.R1 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVGSISDSLLLYRD GQWVCKTKVEKSDS*KGK*TGRYFKLSQLY*PWL LPL*FCSAVEVSPASPERLASLFQISFLQPHTR VPWSGLAHSLGTMWAVQGTLHTVKPHEKVLTPF KIRKIMEY*SLRKCFNF*FSLDYIVFIPIQS*NI IFHIFVEEEAHTSKNARGSHVRMQPWS*LILAF VWFCYLCACPSSPKSMWSSNIATVIQYTCLSTQ *AKF*GFKDEKSCCLPCRGCSD**WKQYGVQESR RPLLSRAVDPAEAETIQVTWFQVGQQGDVL*QN LAPKTSTRRKERLHVFHIYSCLNKESRFMARIR RLCW*PVKTNQGSHAG*GRS*PRCLLWCKLVPVL RSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*VQ QIWRF*PAGCTEKHGCGKTQLEHHD*TLQLYRC YQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLSSC GQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQDQ LPHLPPFC**DL*LQGGALGPGPASSETLGA*D SSPYVRAHRDCGLCPGVVCGPHGHCGGHCLHHPR PAFSWCFQTPRAIVNPILEGKVHRHLQEQKNGL AK*PSTILWPDLSYPFSPPNISPLTFSLGLKLL YPLRAHKCLYILSLTS*FFFSFLKCYLQRHAWGK PPGYLIPQ*PPSKISKEAINSFYEIYVKFFHLS SRAD*NYG**LGYSYVSIQFNLISQIIFHVQ*H RSHQVQYSLIIC*FLS*H*YFLLPCVPTLGTATH PSIQATMKLMDTVCPWPRIVIAKILEPKNKSVL ISMWLLKV*QRNKLG*RKFESQKYQK*KCILKT LNL*RMMTVEAFLFPCHLEIKIL*AGKEMEVRKT LE*DNNVGV*KGTNTYSSHRVSDNGKRDGSRSH RHI*EP*IEAQSASPPE*SCTR*PCSFPCATLA *RRQYSEYGYRMFLGNMPISSEIPSPFP*PLLTK ESMSL*EKP*EEQHS*DNVAAAVGAVSSTGFAI SEEKLSQRKWVSSDLMALNSYEIHEDISNHATC ISEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPNPG LSTLKLVF*TL*MCEVSNISGLTFLIHNVGVII LSLQSY**NLNNFDI*SMPYT*YMNT*ENIVVI FIINLFKRMDHII*KALLFFSAS**FRRFKCRAM GEFLFI**LKDIFLFPE*ERLRLDW*ENS*H*K L*YLTLVFNSLRGNIFPPYGP*VFIQGISYTTD LYACLLWGRSEDSSRRK*FKTQTGNQ*AQEI*TS DDHENVHHRAQMIFRAIKLFCLIPQW*KMSLYI CSNPQNI*HQELDLM*TMDFG***CANVSS*II ANVPLRWEMLLMGEAMHVWEQSVSGSSPQPFWQQ GPVLWKTIFPQIVGGMGV*SGDETVKLFHLRST SIS*ILIRNMQPRSLACTVKNRVRTPMRI*CHH *SDGRRGSVGNAGSPTAWSSF*QATNWYWSMARG LGTSGIPLHLLLSFSVNLQPL*NKVYF*KGKEK VTNYDPKCVK*KF*YEKQSH*NAQRAKKLLAIG FQIYFIYISMIREARTQHFGVVCICVCVCVCVCV SVI*GVY*MA**PESCRNLQMLPNSDSFLKQHC GKQI***QYFIKRMYSKNIYVMLK*QN*N*F*K IRVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS*T II*AI*IISDYTHKV*LH*YLISHYLKFTKLVG HRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNKAR PCLYNTKIN*CMAALL*SQLL*RLRQEECLSPGV QYFSGLRLCHCAHSSLGSRAGLHL*K*INK*NL QNFKNQMKYVRFVPTTFKLGT*RIKHFITNEAL HAQTGT**VVDRC*QFVLLLFSGVQLTNPTVNDI TGMQPTRSRIWKLLLDKQLHFFSNNSREREKRS YTF*KAEEIYEPNLYEAIRKTDTDCIKELSNFS VVMRLLLCFLKSHYTLDFHNKIIMNE

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWDPSLTPCSFIEM DSGFVKQKLKSQIVKRGSELEGTLNFHNFINRG CSHYDFVRQWKFHLLLQSAWRLCSKFPFFNLTPE CPGQAWLIH*AQCGQCRGPSIL*SHMRRF*LLL KLEK*WNIRA*ENVLTFNSA*IILSLYQFSHKI *FSIFLWRKRPIQARMLGAHMSECSLGHD*SWPS YGSATCVPARLPQNLCGPRI*QLSFNTHV*AHS ELSFKDSKMKSHAVFPAEGAQISDGNSMGCRKA EGHC*AGQWTQQRLKQYK*LGSRWASRVTSSSKI WHPRQVPEEKKDCMYSTYIHV*TRSQDLWQG*G GFVGDLLRPTRAVMLDREEADHVASCGVNLYQF YGPSGQYTHEFDGDEQFYVDLERKETAWRWPEFS KFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAA TNEVPEVTVFSKSPVTLGQPNTLICLVDNIFPP VVNITWLSNGQSVTEGVSETSFLSKSDHSFFKIS YLTFLPSADEIYDCKVEHWGLDQPLLKHWEPEI PAPMSELTETVVCALGLSVGLMGIVVGTVFIIQ GLRSVGASRHQGPL*IPSWKGRCIAIYRSRRMDL LNDLALFSGPIYHIPFLLQIFLLSPFLWDLSCY IPSELTNAFTFFP*PPDFFFLFSNVTYKDMPGV SHPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSIFH PGLTETMANNWGTLMFQSNLTSFPRSFFMSSNT EATKYSIA**YVDFLADINISCFLVFPPLALPP TPQFRQQ*N*WIPSALGPELL*QKF*NQKISLY* FQCGF*KYDREIS*DKGNLNLKNIKSKNVFSKL *IYEE**Q*KPSSSLVTLR*KFFRQEKKWKSEK H*NKTIMWVSEKEQILIPHIGLVTMGKGTGVEAT DISRSPE*RRSLPHLLNEAVLDNHVAFPVPLWH EGDSIVNMATGCFLETCQYLQKSPALSPNPS*L RKA*AYERNPRKNNTVETM*QQQWVLCPPLDSPS PRRNSHRGNGSAATSWL*TAMKSMRIFLTMLPA SVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQIQ DSVL*S*YFKLYKCVKSLTFLALLFSSTM*E**Y FPCRVIDKI*IILIFSQCLTHST*THKKTLWLY L*LIYLKEWIILYEKHFCFSQPLNDLGDSNVEL WVNFFSYDN*RIFFFFQNERG*DWIGKRTLSTRS CNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQQI FMPVYFGEEVKIVQGENNLKHRLGISKHRKSEP VMIMKMSITEHR*FSGQ*NYSV*YHNGEKCHYTF AQIHRIYNTKS*T*CKLWTLGDNDVPM*VHKL* QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLFGS RDQFYGRQFFHR*LGEWGYDLGMKL*NYSTSDQQ ALVRFS*GTCNLDLLHAQLKIGFALL*ESNATT DLTEGGAQLVMLAHPLRGPVSNRPPTGTGPWPG GWGPLGYLCTFCSVFL*TYNRFKIRSIFKKEKRR *PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q*G FKYISYTFQ*FVRQEPSILELCAFVCVCVCVCV CL*YKGYTEWHNDQSHAEIYKCCPTQTPSSSSTV ESKFNDSSILLKGCIQKIFT*C*NSRIKTNSKK *E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAILK QLFELFELFQITHIKCDFINT*YHTI*NLQN*WV TEARACDLSTLEANRGGLLEARSSTAAWATKQD LVSTTQK*INAWRHSCSPSYSRG*GKRSA*AQE FNTSVG*DCATVLTPA*AAEQDSISKSK*INKTY KILKIK*NMSGLYLPHLN*GLEELNILLQMKHF MHRLAHSK*SIGVNNLCYCYFLESN*QIPQ*MT SQGCNQQDPEYGNFY*INNSISSATIQERERREA IHFKRLKKYMNQICMRQSEKLTPTVLRNYLILV W**DCCYVF*KVIIL*IFIIK*L*MK

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCGIHL*LPAPL*RW TVGL*NKS*KVR*LKGEVNWKVL*TFTTLLTVA APIMILFGSGSFTCFSRALGVFVPNFLSSTSHQ SALVRLGSFIRHNVGSAGDPPYCKAT*EGFNSF* N*KNNGILEPKKMF*LLIQPRLYCLYTNSVIKY NFPYFCGGRGPYKQECSGLTCQNAALVMTDPGL RMVLLPVCLPVFPKIYVVLEYSNCHSIHMFEHTV S*VLRIQR*KVMLSSLQRVLRLVMETVWGAGKQ KAIAEQGSGPSRG*NNTSDLVPGGPAG*RPLAK FGTQDKYQKKRKTACIPHIFMFEQGVKIYGKDKE ALLVTC*DQPGQSCWIGKKLTTLPLVV*TCTSF TVPLASTPMNLMEMSSSTWTWRGRRLPGGGLSS ANLEVLTRRVH*ETWLWQNTT*TS*LNATTLPLL PMRFLRSQCFPSLP*HWVSPTPSFVLWTTSFLL WSTSHG*AMGSQSQKVFLRPASSPRVIIPSSRS VTSPSSLLLMRFMTARWSTGAWTSLF*NTGSLRF QPLCQSSQRLWSVPWGCLWASWALWWALSSSSK ACVQLVLPDTKGHCESHPGREGASPSTGAEEWT C*MT*HYSLARFIISLFSSKYFSSHLFSGT*AAI SPQSSQMPLHSFPDLLIFFFFSQMLPTKTCLG* ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFPSF IQG*LKLWLIIGVLLCFNPI*PHFPDHFSCPVTQ KPPSTV*PDNMLIS*LTLIFLASLCSHPWHCHP PLNSGNNEINGYRLPLAQNCYSKNFRTKK*VCT NFNVAFKSMTEK*VRIKEI*ISKISKVKMYSQNF KFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQKN IRIRQ*CGCLKRNKYLFLT*G**QWEKGRE*KP QTYLGALNRGAVCLTS*MKLY*ITM*LSLCHSGM KETV**IWLQDVSWKHANIFRNPQPFPLTPPD* GKHELMRETLGRTTQLRQCSSSSGCCVLHWIRH LRGETLTEEMGQQRPHGSKQL*NP*GYF*PCYLH Q*V*ILIGEKIQNINAIIDTV*FGAKNPKSKSR TQYFEASILNFINV*SL*HFWPYFSHPQCRSNN TFLAELLIKFE*F*YLVNALHIVHEHIRKHCGYI YN*FI*KNGSYYMKSTFVFLSLLMI*EIQM*SY G*ISFHMIIEGYFSFSRMREAEIGLVRELLALE VVIFDFGFQLSKREYIPSLWPISVYSRYIIYNRS LCLFTLGKK*R*FKEKII*NTDWESVSTGNLNQ **S*KCPSQSTDDFQGNKTILFDTTMVKNVIIH LLKSTEYITPRVRLNVNYGLWVIMMCQCKFINYS KCTTQMGDVANGGGYACVGAECIWQQSPAFLAA GTSFMEDNFSTDSWGNGGMIWG*NCETIPPQIN KH*LDSHKEHAT*ISCMHS*K*GSHSYENLMPPL I*RKAGLSW*CWLTHCVVQFLTGHQLVLVHGPG VGDLWDTSAPSAQFFCEPTTALK*GLFLKRKRE GNQL*S*MCKIKILV*KTVTLKCTTC*EAFSNRV SNIFHIHFNDS*GKNPAFWSCVHLCVCVCVCVC VCNIRGILNGIMTRVMQKSTNAAQLRLLPQAAL WKANLMIAVFY*KDVFKKYLRNVKIAELKLILKN KSK*FFLSAKNVGPTINRVTDLPA*FKIMQFLN NYLSYLNYFRLHT*SVTSLILNITLFKIYKISG SQRLVLVILALWRPIGEDCLKPGVQQLPGQQSKT LSLQHKNKLMHGGTPVVPATLEAEARGVLEPRS SILQWAKIVPLCSLQPRQQSRTPSLKVNK*IKL TKF*KSNEICQVCTYHI*TRDLKN*TFYYK*STS CTDWHIVSSR*VLTICVIVIFWSPTNKSHSE*H HRDATNKIQNMETSTR*TTPFLQQQFKREREEK LYILKG*RNI*TKFV*GNQKN*HRLY*GII*F*C GNEIAVMFSKKSLYFRFS**NNYE*