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Sequences

Sequence HLA-DQA1.fAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGGTGCA TCGCCATCTACAGGAGCAGAAGAATGGACTTGCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCT CTCACCTTTTCTCTGGGACTTAAGCTGCTATATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCA AATGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTA TGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATT TCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTC CTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAA AATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAA TATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTT AGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGA CAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAA CCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCC CCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGT GGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATC CATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTT TGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTAC TTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTAC ATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTT AATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATT GGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTG TTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTG GGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGAT ACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAA TGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTAT CTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGA AACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTT CGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGG CCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAA TAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCA CAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCA TTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCC CAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTA AAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACC TGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATC ACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAG TTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGC TGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCA TCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGA ATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAG TCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAG CAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACC GACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTA TGAATGAAA

HLA-DQA1.F3 (forward primer)
HLA-DQA1.R3 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVADHVASCGVNLY QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWGA SPSTGAEEWTC*MT*HYSLARFIISLFSSKYFS SHLFSGT*AAISPQSSQMPLHSFPDLLIFFFFSQ MLPTKTCLG*ATRLPNSSVTSI*NLQGSNKFLL *DLCQIFPSFIQG*LKLWLIIGVLLCFNPI*PH FPDHFSCPVTQKPPSTV*PDNMLIS*LTLIFLAS LCSHPWHCHPPLNSGNNEINGYRLPLAQNCYSK NFRTKK*VCTNFNVAFKSMTEK*VRIKEI*ISK ISKVKMYSQNFKFMKNDDSRSLPLPLSP*DKNSL GRKRNGSQKNIRIRQ*CGCLKRNKYLFLT*G** QWEKGRE*KPQTYLGALNRGAVCLTS*MKLY*I TM*LSLCHSGMKETV**IWLQDVSWKHANIFRNP QPFPLTPPD*GKHELMRETLGRTTQLRQCSSSS GCCVLHWIRHLRGETLTEEMGQQRPHGSKQL*N P*GYF*PCYLHQ*V*ILIGEKIQNINAIIDTV*F GAKNPKSKSRTQYFEASILNFINV*SL*HFWPY FSHPQCRSNNTFLAELLIKFE*F*YLVNALHIV HEHIRKHCGYIYN*FI*KNGSYYMKSTFVFLSLL MI*EIQM*SYG*ISFHMIIEGYFSFSRMREAEI GLVRELLALEVVIFDFGFQLSKREYIPSLWPIS VYSRYIIYNRSLCLFTLGKK*R*FKEKII*NTDW ESVSTGNLNQ**S*KCPSQSTDDFQGNKTILFD TTMVKNVIIHLLKSTEYITPRVRLNVNYGLWVI MMCQCKFINYSKCTTQMGDVANGGGYACVGAECI WQQSPAFLAAGTSFMEDNFSTDSWGNGGMIWG* NCETIPPQINKH*LDSHKEHAT*ISCMHS*K*G SHSYENLMPPLI*RKAGLSW*CWLTHCVVQFLTG HQLVLVHGPGVGDLWDTSAPSAQFFCEPTTALK *GLFLKRKREGNQL*S*MCKIKILV*KTVTLKC TTC*EAFSNRVSNIFHIHFNDS*GKNPAFWSCVH LCVCVCVCVCVCNIRGILNGIMTRVMQKSTNAA QLRLLPQAALWKANLMIAVFY*KDVFKKYLRNV KIAELKLILKNKSK*FFLSAKNVGPTINRVTDLP A*FKIMQFLNNYLSYLNYFRLHT*SVTSLILNI TLFKIYKISGSQRLVLVILALWRPIGEDCLKPG VQQLPGQQSKTLSLQHKNKLMHGGTPVVPATLEA EARGVLEPRSSILQWAKIVPLCSLQPRQQSRTP SLKVNK*IKLTKF*KSNEICQVCTYHI*TRDLK N*TFYYK*STSCTDWHIVSSR*VLTICVIVIFWS PTNKSHSE*HHRDATNKIQNMETSTR*TTPFLQ QQFKREREEKLYILKG*RNI*TKFV*GNQKN*H RLY*GII*F*CGNEIAVMFSKKSLYFRFS**NNY E*

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGVH RHLQEQKNGLAK*PSTILWPDLSYPFSPPNISP LTFSLGLKLLYPLRAHKCLYILSLTS*FFFSFL KCYLQRHAWGKPPGYLIPQ*PPSKISKEAINSFY EIYVKFFHLSSRAD*NYG**LGYSYVSIQFNLI SQIIFHVQ*HRSHQVQYSLIIC*FLS*H*YFLL PCVPTLGTATHPSIQATMKLMDTVCPWPRIVIAK ILEPKNKSVLISMWLLKV*QRNKLG*RKFESQK YQK*KCILKTLNL*RMMTVEAFLFPCHLEIKIL *AGKEMEVRKTLE*DNNVGV*KGTNTYSSHRVSD NGKRDGSRSHRHI*EP*IEAQSASPPE*SCTR* PCSFPCATLA*RRQYSEYGYRMFLGNMPISSEI PSPFP*PLLTKESMSL*EKP*EEQHS*DNVAAAV GAVSSTGFAISEEKLSQRKWVSSDLMALNSYEI HEDISNHATCISEFKF*LEKKYKTLMQ*LIQYS LVQRTLNPNPGLSTLKLVF*TL*MCEVSNISGLT FLIHNVGVIILSLQSY**NLNNFDI*SMPYT*Y MNT*ENIVVIFIINLFKRMDHII*KALLFFSAS **FRRFKCRAMGEFLFI**LKDIFLFPE*ERLRL DW*ENS*H*KL*YLTLVFNSLRGNIFPPYGP*V FIQGISYTTDLYACLLWGRSEDSSRRK*FKTQT GNQ*AQEI*TSDDHENVHHRAQMIFRAIKLFCLI PQW*KMSLYICSNPQNI*HQELDLM*TMDFG** *CANVSS*IIANVPLRWEMLLMGEAMHVWEQSV SGSSPQPFWQQGPVLWKTIFPQIVGGMGV*SGDE TVKLFHLRSTSIS*ILIRNMQPRSLACTVKNRV RTPMRI*CHH*SDGRRGSVGNAGSPTAWSSF*Q ATNWYWSMARGLGTSGIPLHLLLSFSVNLQPL*N KVYF*KGKEKVTNYDPKCVK*KF*YEKQSH*NA QRAKKLLAIGFQIYFIYISMIREARTQHFGVVC ICVCVCVCVCVSVI*GVY*MA**PESCRNLQMLP NSDSFLKQHCGKQI***QYFIKRMYSKNIYVML K*QN*N*F*KIRVNSSFYQLKM*GLLSIGLQIY LPDLKSCNS*TII*AI*IISDYTHKV*LH*YLIS HYLKFTKLVGHRGSCL*S*HFGGQ*GRIA*SQE FNSCLGNKARPCLYNTKIN*CMAALL*SQLL*R LRQEECLSPGVQYFSGLRLCHCAHSSLGSRAGLH L*K*INK*NLQNFKNQMKYVRFVPTTFKLGT*R IKHFITNEALHAQTGT**VVDRC*QFVLLLFSG VQLTNPTVNDITGMQPTRSRIWKLLLDKQLHFFS NNSREREKRSYTF*KAEEIYEPNLYEAIRKTDT DCIKELSNFSVVMRLLLCFLKSHYTLDFHNKII MNE

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA* VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ DQLPHLPPFC**DL*LQGGALGPGPASSETLGC IAIYRSRRMDLLNDLALFSGPIYHIPFLLQIFLL SPFLWDLSCYIPSELTNAFTFFP*PPDFFFLFS NVTYKDMPGVSHPAT*FLSNLHLKSPRKQ*IPF MRSMSNFSIFHPGLTETMANNWGTLMFQSNLTSF PRSFFMSSNTEATKYSIA**YVDFLADINISCF LVFPPLALPPTPQFRQQ*N*WIPSALGPELL*Q KF*NQKISLY*FQCGF*KYDREIS*DKGNLNLKN IKSKNVFSKL*IYEE**Q*KPSSSLVTLR*KFF RQEKKWKSEKH*NKTIMWVSEKEQILIPHIGLV TMGKGTGVEATDISRSPE*RRSLPHLLNEAVLDN HVAFPVPLWHEGDSIVNMATGCFLETCQYLQKS PALSPNPS*LRKA*AYERNPRKNNTVETM*QQQ WVLCPPLDSPSPRRNSHRGNGSAATSWL*TAMKS MRIFLTMLPASVSLNFNWRKNTKH*CNN*YSIV WCKEP*IQIQDSVL*S*YFKLYKCVKSLTFLAL LFSSTM*E**YFPCRVIDKI*IILIFSQCLTHST *THKKTLWLYL*LIYLKEWIILYEKHFCFSQPL NDLGDSNVELWVNFFSYDN*RIFFFFQNERG*D WIGKRTLSTRSCNI*LWFSTL*EGIYSLLMAHKC LFKVYHIQQIFMPVYFGEEVKIVQGENNLKHRL GISKHRKSEPVMIMKMSITEHR*FSGQ*NYSV* YHNGEKCHYTFAQIHRIYNTKS*T*CKLWTLGDN DVPM*VHKL*QMYHSDGRCC*WGRLCMCGSRVY LAAVPSLFGSRDQFYGRQFFHR*LGEWGYDLGM KL*NYSTSDQQALVRFS*GTCNLDLLHAQLKIGF ALL*ESNATTDLTEGGAQLVMLAHPLRGPVSNR PPTGTGPWPGGWGPLGYLCTFCSVFL*TYNRFK IRSIFKKEKRR*PIMILNV*NKNFSMKNSHIKMH NVLRSF*Q*GFKYISYTFQ*FVRQEPSILELCA FVCVCVCVCVCL*YKGYTEWHNDQSHAEIYKCC PTQTPSSSSTVESKFNDSSILLKGCIQKIFT*C* NSRIKTNSKK*E*IVLFIS*KCRAYYQ*GYRST CLI*NHAILKQLFELFELFQITHIKCDFINT*Y HTI*NLQN*WVTEARACDLSTLEANRGGLLEARS STAAWATKQDLVSTTQK*INAWRHSCSPSYSRG *GKRSA*AQEFNTSVG*DCATVLTPA*AAEQDS ISKSK*INKTYKILKIK*NMSGLYLPHLN*GLEE LNILLQMKHFMHRLAHSK*SIGVNNLCYCYFLE SN*QIPQ*MTSQGCNQQDPEYGNFY*INNSISS ATIQERERREAIHFKRLKKYMNQICMRQSEKLTP TVLRNYLILVW**DCCYVF*KVIIL*IFIIK*L *MK

Sequence HLA-DQA1.iAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC GCTGCTACCAATGGTATGCGTCCACCATTCTGCCTCTCTTTACTTAAGTTATCCCTCCATACCAGGGTTCATTATTTTCTTCCCAAGAGGTCCCCAGATC TTCTTATGGCAATTGCTGAAATTTTATCATCTCCCATCTCTAAAATCACATATTCCCATGTAATACAAGGGTCTTTCCATTATGCATTCAGCAAATCCTT CTAGGAGAGGTCTCATCAACCTCCTACTTTATTAAACATGCCCACAGAGAGAAGGGCACAGGAATAAAGCAGAGGCAATGTGTCGTTGCTCCCAAGCAGA AGGTAAATAAGACCTCTTTGACTATCAGGTGGTGAAATGCTGGTAGGAGGGCTCTTCCAGGATGTAATGCAGAACTTCAGGGCAGAGCTATTCACACTTC ACACCAGTGCTGTTTCCTCACCACAGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTG GACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATC ATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAA ACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGC ACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTAAGATTGAGACTGGT TACAGTTGAAGCGGCAGTATGAAAGGAAGGAAAGTGGGAGGGCGTTGTGGACATGAATGTGGTTGAAAGTTGTAGGGGAATTGGGAAGTGGCATGATGAT GACACAGGAGCCCCCTCGGACCCATTGATCTCATGTCTGCCCTGTTGCAGGTGCATCGCCATCTACAGGAGCAGAAGAATGGACTTGCTAAATGACCTAG CACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGCTGCTATATCCCCTCAGAGCT CACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACC TAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTG AAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTA CAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGG CAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCT TTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGA ATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACA ATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGA GCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTAT AGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCT TATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACT CTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAA TTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGA AGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAG TTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTA TTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTT TCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGAC TTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTA CTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAA ATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACA GAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTC AGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTAT GGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGAT TCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGG CGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTG GGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGA TCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTC ATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAG GGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTA ATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGT TCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTA TTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTG CTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAAC ACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTG GGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAA TCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGC ACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAAC CAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAA GGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTG CTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F3 (forward primer)
HLA-DQA1.R3 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVADHVASCGVNLY QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST AATNGMRPPFCLSLLKLSLHTRVHYFLPKRSPD LLMAIAEILSSPISKITYSHVIQGSFHYAFSKSF *ERSHQPPTLLNMPTERRAQE*SRGNVSLLPSR R*IRPL*LSGGEMLVGGLFQDVMQNFRAELFTL HTSAVSSPQRFLRSQCFPSLP*HWVSPTPSFVLW TTSFLLWSTSHG*AMGSQSQKVFLRPASSPRVI IPSSRSVTSPSSLLLMRFMTARWSTGAWTSLF* NTGSLRFQPLCQSSQRLWSVPWGCLWASWALWWA LSSSSKACVQLVLPDTKGHCESHPGREGKIETG YS*SGSMKGRKVGGRCGHECG*KL*GNWEVA** *HRSPLGPIDLMSALLQVHRHLQEQKNGLAK*PS TILWPDLSYPFSPPNISPLTFSLGLKLLYPLRA HKCLYILSLTS*FFFSFLKCYLQRHAWGKPPGY LIPQ*PPSKISKEAINSFYEIYVKFFHLSSRAD* NYG**LGYSYVSIQFNLISQIIFHVQ*HRSHQV QYSLIIC*FLS*H*YFLLPCVPTLGTATHPSIQ ATMKLMDTVCPWPRIVIAKILEPKNKSVLISMWL LKV*QRNKLG*RKFESQKYQK*KCILKTLNL*R MMTVEAFLFPCHLEIKIL*AGKEMEVRKTLE*D NNVGV*KGTNTYSSHRVSDNGKRDGSRSHRHI*E P*IEAQSASPPE*SCTR*PCSFPCATLA*RRQY SEYGYRMFLGNMPISSEIPSPFP*PLLTKESMS L*EKP*EEQHS*DNVAAAVGAVSSTGFAISEEKL SQRKWVSSDLMALNSYEIHEDISNHATCISEFK F*LEKKYKTLMQ*LIQYSLVQRTLNPNPGLSTL KLVF*TL*MCEVSNISGLTFLIHNVGVIILSLQS Y**NLNNFDI*SMPYT*YMNT*ENIVVIFIINL FKRMDHII*KALLFFSAS**FRRFKCRAMGEFL FI**LKDIFLFPE*ERLRLDW*ENS*H*KL*YLT LVFNSLRGNIFPPYGP*VFIQGISYTTDLYACL LWGRSEDSSRRK*FKTQTGNQ*AQEI*TSDDHE NVHHRAQMIFRAIKLFCLIPQW*KMSLYICSNPQ NI*HQELDLM*TMDFG***CANVSS*IIANVPL RWEMLLMGEAMHVWEQSVSGSSPQPFWQQGPVL WKTIFPQIVGGMGV*SGDETVKLFHLRSTSIS*I LIRNMQPRSLACTVKNRVRTPMRI*CHH*SDGR RGSVGNAGSPTAWSSF*QATNWYWSMARGLGTS GIPLHLLLSFSVNLQPL*NKVYF*KGKEKVTNYD PKCVK*KF*YEKQSH*NAQRAKKLLAIGFQIYF IYISMIREARTQHFGVVCICVCVCVCVCVSVI* GVY*MA**PESCRNLQMLPNSDSFLKQHCGKQI* **QYFIKRMYSKNIYVMLK*QN*N*F*KIRVNS SFYQLKM*GLLSIGLQIYLPDLKSCNS*TII*A I*IISDYTHKV*LH*YLISHYLKFTKLVGHRGSC L*S*HFGGQ*GRIA*SQEFNSCLGNKARPCLYN TKIN*CMAALL*SQLL*RLRQEECLSPGVQYFS GLRLCHCAHSSLGSRAGLHL*K*INK*NLQNFKN QMKYVRFVPTTFKLGT*RIKHFITNEALHAQTG T**VVDRC*QFVLLLFSGVQLTNPTVNDITGMQ PTRSRIWKLLLDKQLHFFSNNSREREKRSYTF*K AEEIYEPNLYEAIRKTDTDCIKELSNFSVVMRL LLCFLKSHYTLDFHNKIIMNE

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP LLPMVCVHHSASLYLSYPSIPGFIIFFPRGPQI FLWQLLKFYHLPSLKSHIPM*YKGLSIMHSANP SRRGLINLLLY*TCPQREGHRNKAEAMCRCSQAE GK*DLFDYQVVKCW*EGSSRM*CRTSGQSYSHF TPVLFPHHRGS*GHSVFQVSRDTGSAQHPHLSC GQHLSSCGQHHMAEQWAVSHRRCF*DQLPLQE*S FLLQDQLPHLPPFC**DL*LQGGALGPGPASSE TLGA*DSSPYVRAHRDCGLCPGVVCGPHGHCGG HCLHHPRPAFSWCFQTPRAIVNPILEGKVRLRLV TVEAAV*KEGKWEGVVDMNVVESCRGIGKWHDD DTGAPSDPLISCLPCCRCIAIYRSRRMDLLNDL ALFSGPIYHIPFLLQIFLLSPFLWDLSCYIPSEL TNAFTFFP*PPDFFFLFSNVTYKDMPGVSHPAT *FLSNLHLKSPRKQ*IPFMRSMSNFSIFHPGLT ETMANNWGTLMFQSNLTSFPRSFFMSSNTEATKY SIA**YVDFLADINISCFLVFPPLALPPTPQFR QQ*N*WIPSALGPELL*QKF*NQKISLY*FQCG F*KYDREIS*DKGNLNLKNIKSKNVFSKL*IYEE **Q*KPSSSLVTLR*KFFRQEKKWKSEKH*NKT IMWVSEKEQILIPHIGLVTMGKGTGVEATDISR SPE*RRSLPHLLNEAVLDNHVAFPVPLWHEGDSI VNMATGCFLETCQYLQKSPALSPNPS*LRKA*A YERNPRKNNTVETM*QQQWVLCPPLDSPSPRRN SHRGNGSAATSWL*TAMKSMRIFLTMLPASVSLN FNWRKNTKH*CNN*YSIVWCKEP*IQIQDSVL* S*YFKLYKCVKSLTFLALLFSSTM*E**YFPCR VIDKI*IILIFSQCLTHST*THKKTLWLYL*LIY LKEWIILYEKHFCFSQPLNDLGDSNVELWVNFF SYDN*RIFFFFQNERG*DWIGKRTLSTRSCNI* LWFSTL*EGIYSLLMAHKCLFKVYHIQQIFMPVY FGEEVKIVQGENNLKHRLGISKHRKSEPVMIMK MSITEHR*FSGQ*NYSV*YHNGEKCHYTFAQIH RIYNTKS*T*CKLWTLGDNDVPM*VHKL*QMYHS DGRCC*WGRLCMCGSRVYLAAVPSLFGSRDQFY GRQFFHR*LGEWGYDLGMKL*NYSTSDQQALVR FS*GTCNLDLLHAQLKIGFALL*ESNATTDLTEG GAQLVMLAHPLRGPVSNRPPTGTGPWPGGWGPL GYLCTFCSVFL*TYNRFKIRSIFKKEKRR*PIM ILNV*NKNFSMKNSHIKMHNVLRSF*Q*GFKYIS YTFQ*FVRQEPSILELCAFVCVCVCVCVCL*YK GYTEWHNDQSHAEIYKCCPTQTPSSSSTVESKF NDSSILLKGCIQKIFT*C*NSRIKTNSKK*E*IV LFIS*KCRAYYQ*GYRSTCLI*NHAILKQLFEL FELFQITHIKCDFINT*YHTI*NLQN*WVTEAR ACDLSTLEANRGGLLEARSSTAAWATKQDLVSTT QK*INAWRHSCSPSYSRG*GKRSA*AQEFNTSV G*DCATVLTPA*AAEQDSISKSK*INKTYKILK IK*NMSGLYLPHLN*GLEELNILLQMKHFMHRLA HSK*SIGVNNLCYCYFLESN*QIPQ*MTSQGCN QQDPEYGNFY*INNSISSATIQERERREAIHFK RLKKYMNQICMRQSEKLTPTVLRNYLILVW**DC CYVF*KVIIL*IFIIK*L*MK

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA* VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY RCYQWYASTILPLFT*VIPPYQGSLFSSQEVPRS SYGNC*NFIISHL*NHIFPCNTRVFPLCIQQIL LGEVSSTSYFIKHAHREKGTGIKQRQCVVAPKQ KVNKTSLTIRW*NAGRRALPGCNAELQGRAIHTS HQCCFLTTEVPEVTVFSKSPVTLGQPNTLICLV DNIFPPVVNITWLSNGQSVTEGVSETSFLSKSD HSFFKISYLTFLPSADEIYDCKVEHWGLDQPLLK HWEPEIPAPMSELTETVVCALGLSVGLMGIVVG TVFIIQGLRSVGASRHQGPL*IPSWKGR*D*DW LQLKRQYERKESGRALWT*MWLKVVGELGSGMMM TQEPPRTH*SHVCPVAGASPSTGAEEWTC*MT* HYSLARFIISLFSSKYFSSHLFSGT*AAISPQS SQMPLHSFPDLLIFFFFSQMLPTKTCLG*ATRLP NSSVTSI*NLQGSNKFLL*DLCQIFPSFIQG*L KLWLIIGVLLCFNPI*PHFPDHFSCPVTQKPPS TV*PDNMLIS*LTLIFLASLCSHPWHCHPPLNSG NNEINGYRLPLAQNCYSKNFRTKK*VCTNFNVA FKSMTEK*VRIKEI*ISKISKVKMYSQNFKFMK NDDSRSLPLPLSP*DKNSLGRKRNGSQKNIRIRQ *CGCLKRNKYLFLT*G**QWEKGRE*KPQTYLG ALNRGAVCLTS*MKLY*ITM*LSLCHSGMKETV **IWLQDVSWKHANIFRNPQPFPLTPPD*GKHEL MRETLGRTTQLRQCSSSSGCCVLHWIRHLRGET LTEEMGQQRPHGSKQL*NP*GYF*PCYLHQ*V* ILIGEKIQNINAIIDTV*FGAKNPKSKSRTQYFE ASILNFINV*SL*HFWPYFSHPQCRSNNTFLAE LLIKFE*F*YLVNALHIVHEHIRKHCGYIYN*F I*KNGSYYMKSTFVFLSLLMI*EIQM*SYG*ISF HMIIEGYFSFSRMREAEIGLVRELLALEVVIFD FGFQLSKREYIPSLWPISVYSRYIIYNRSLCLF TLGKK*R*FKEKII*NTDWESVSTGNLNQ**S*K CPSQSTDDFQGNKTILFDTTMVKNVIIHLLKST EYITPRVRLNVNYGLWVIMMCQCKFINYSKCTT QMGDVANGGGYACVGAECIWQQSPAFLAAGTSFM EDNFSTDSWGNGGMIWG*NCETIPPQINKH*LD SHKEHAT*ISCMHS*K*GSHSYENLMPPLI*RK AGLSW*CWLTHCVVQFLTGHQLVLVHGPGVGDLW DTSAPSAQFFCEPTTALK*GLFLKRKREGNQL* S*MCKIKILV*KTVTLKCTTC*EAFSNRVSNIF HIHFNDS*GKNPAFWSCVHLCVCVCVCVCVCNIR GILNGIMTRVMQKSTNAAQLRLLPQAALWKANL MIAVFY*KDVFKKYLRNVKIAELKLILKNKSK* FFLSAKNVGPTINRVTDLPA*FKIMQFLNNYLSY LNYFRLHT*SVTSLILNITLFKIYKISGSQRLV LVILALWRPIGEDCLKPGVQQLPGQQSKTLSLQ HKNKLMHGGTPVVPATLEAEARGVLEPRSSILQW AKIVPLCSLQPRQQSRTPSLKVNK*IKLTKF*K SNEICQVCTYHI*TRDLKN*TFYYK*STSCTDW HIVSSR*VLTICVIVIFWSPTNKSHSE*HHRDAT NKIQNMETSTR*TTPFLQQQFKREREEKLYILK G*RNI*TKFV*GNQKN*HRLY*GII*F*CGNEI AVMFSKKSLYFRFS**NNYE*

Sequence HLA-DQA1.kAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACAC TGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGT TTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTG GAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGT TGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCC ATCCTGGAAGGGAAGGTAAGATTGAGACTGGTTACAGTTGAAGCGGCAGTATGAAAGGAAGGAAAGTGGGAGGGCGTTGTGGACATGAATGTGGTTGAAA GTTGTAGGGGAATTGGGAAGTGGCATGATGATGACACAGGAGCCCCCTCGGACCCATTGATCTCATGTCTGCCCTGTTGCAGGTGCATCGCCATCTACAG GAGCAGAAGAATGGACTTGCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTC TGGGACTTAAGCTGCTATATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACA AAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTC AAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTT TTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCAC CCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCA AAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAA AATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGA AATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGG GACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTC CCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCC CTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCC TCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTT CTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAAC CCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCAC AATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAG AAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAG ATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAG AACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTA TATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGC ACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGA AAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATG TAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCC CCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTA TTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGA GAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTA CTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTT TAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGA AGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGT GTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCC TTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATT AAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAA ATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAA TTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCC TGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGA GTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAA ATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTA TTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAA TCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGA GAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGG AATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F3 (forward primer)
HLA-DQA1.R3 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVADHVASCGVNLY QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE FSKFGGFDPQGALRNMAVARFLRSQCFPSLP*H WVSPTPSFVLWTTSFLLWSTSHG*AMGSQSQKV FLRPASSPRVIIPSSRSVTSPSSLLLMRFMTARW STGAWTSLF*NTGSLRFQPLCQSSQRLWSVPWG CLWASWALWWALSSSSKACVQLVLPDTKGHCES HPGREGKIETGYS*SGSMKGRKVGGRCGHECG*K L*GNWEVA***HRSPLGPIDLMSALLQVHRHLQ EQKNGLAK*PSTILWPDLSYPFSPPNISPLTFS LGLKLLYPLRAHKCLYILSLTS*FFFSFLKCYLQ RHAWGKPPGYLIPQ*PPSKISKEAINSFYEIYV KFFHLSSRAD*NYG**LGYSYVSIQFNLISQII FHVQ*HRSHQVQYSLIIC*FLS*H*YFLLPCVPT LGTATHPSIQATMKLMDTVCPWPRIVIAKILEP KNKSVLISMWLLKV*QRNKLG*RKFESQKYQK* KCILKTLNL*RMMTVEAFLFPCHLEIKIL*AGKE MEVRKTLE*DNNVGV*KGTNTYSSHRVSDNGKR DGSRSHRHI*EP*IEAQSASPPE*SCTR*PCSF PCATLA*RRQYSEYGYRMFLGNMPISSEIPSPFP *PLLTKESMSL*EKP*EEQHS*DNVAAAVGAVS STGFAISEEKLSQRKWVSSDLMALNSYEIHEDI SNHATCISEFKF*LEKKYKTLMQ*LIQYSLVQRT LNPNPGLSTLKLVF*TL*MCEVSNISGLTFLIH NVGVIILSLQSY**NLNNFDI*SMPYT*YMNT* ENIVVIFIINLFKRMDHII*KALLFFSAS**FRR FKCRAMGEFLFI**LKDIFLFPE*ERLRLDW*E NS*H*KL*YLTLVFNSLRGNIFPPYGP*VFIQG ISYTTDLYACLLWGRSEDSSRRK*FKTQTGNQ*A QEI*TSDDHENVHHRAQMIFRAIKLFCLIPQW* KMSLYICSNPQNI*HQELDLM*TMDFG***CAN VSS*IIANVPLRWEMLLMGEAMHVWEQSVSGSSP QPFWQQGPVLWKTIFPQIVGGMGV*SGDETVKL FHLRSTSIS*ILIRNMQPRSLACTVKNRVRTPM RI*CHH*SDGRRGSVGNAGSPTAWSSF*QATNWY WSMARGLGTSGIPLHLLLSFSVNLQPL*NKVYF *KGKEKVTNYDPKCVK*KF*YEKQSH*NAQRAK KLLAIGFQIYFIYISMIREARTQHFGVVCICVCV CVCVCVSVI*GVY*MA**PESCRNLQMLPNSDS FLKQHCGKQI***QYFIKRMYSKNIYVMLK*QN *N*F*KIRVNSSFYQLKM*GLLSIGLQIYLPDLK SCNS*TII*AI*IISDYTHKV*LH*YLISHYLK FTKLVGHRGSCL*S*HFGGQ*GRIA*SQEFNSC LGNKARPCLYNTKIN*CMAALL*SQLL*RLRQEE CLSPGVQYFSGLRLCHCAHSSLGSRAGLHL*K* INK*NLQNFKNQMKYVRFVPTTFKLGT*RIKHF ITNEALHAQTGT**VVDRC*QFVLLLFSGVQLTN PTVNDITGMQPTRSRIWKLLLDKQLHFFSNNSR EREKRSYTF*KAEEIYEPNLYEAIRKTDTDCIK ELSNFSVVMRLLLCFLKSHYTLDFHNKIIMNE

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL SSANLEVLTRRVH*ETWLWQGS*GHSVFQVSRDT GSAQHPHLSCGQHLSSCGQHHMAEQWAVSHRRC F*DQLPLQE*SFLLQDQLPHLPPFC**DL*LQG GALGPGPASSETLGA*DSSPYVRAHRDCGLCPGV VCGPHGHCGGHCLHHPRPAFSWCFQTPRAIVNP ILEGKVRLRLVTVEAAV*KEGKWEGVVDMNVVE SCRGIGKWHDDDTGAPSDPLISCLPCCRCIAIYR SRRMDLLNDLALFSGPIYHIPFLLQIFLLSPFL WDLSCYIPSELTNAFTFFP*PPDFFFLFSNVTY KDMPGVSHPAT*FLSNLHLKSPRKQ*IPFMRSMS NFSIFHPGLTETMANNWGTLMFQSNLTSFPRSF FMSSNTEATKYSIA**YVDFLADINISCFLVFP PLALPPTPQFRQQ*N*WIPSALGPELL*QKF*NQ KISLY*FQCGF*KYDREIS*DKGNLNLKNIKSK NVFSKL*IYEE**Q*KPSSSLVTLR*KFFRQEK KWKSEKH*NKTIMWVSEKEQILIPHIGLVTMGKG TGVEATDISRSPE*RRSLPHLLNEAVLDNHVAF PVPLWHEGDSIVNMATGCFLETCQYLQKSPALS PNPS*LRKA*AYERNPRKNNTVETM*QQQWVLCP PLDSPSPRRNSHRGNGSAATSWL*TAMKSMRIF LTMLPASVSLNFNWRKNTKH*CNN*YSIVWCKE P*IQIQDSVL*S*YFKLYKCVKSLTFLALLFSST M*E**YFPCRVIDKI*IILIFSQCLTHST*THK KTLWLYL*LIYLKEWIILYEKHFCFSQPLNDLG DSNVELWVNFFSYDN*RIFFFFQNERG*DWIGKR TLSTRSCNI*LWFSTL*EGIYSLLMAHKCLFKV YHIQQIFMPVYFGEEVKIVQGENNLKHRLGISK HRKSEPVMIMKMSITEHR*FSGQ*NYSV*YHNGE KCHYTFAQIHRIYNTKS*T*CKLWTLGDNDVPM *VHKL*QMYHSDGRCC*WGRLCMCGSRVYLAAV PSLFGSRDQFYGRQFFHR*LGEWGYDLGMKL*NY STSDQQALVRFS*GTCNLDLLHAQLKIGFALL* ESNATTDLTEGGAQLVMLAHPLRGPVSNRPPTG TGPWPGGWGPLGYLCTFCSVFL*TYNRFKIRSIF KKEKRR*PIMILNV*NKNFSMKNSHIKMHNVLR SF*Q*GFKYISYTFQ*FVRQEPSILELCAFVCV CVCVCVCL*YKGYTEWHNDQSHAEIYKCCPTQTP SSSSTVESKFNDSSILLKGCIQKIFT*C*NSRI KTNSKK*E*IVLFIS*KCRAYYQ*GYRSTCLI* NHAILKQLFELFELFQITHIKCDFINT*YHTI*N LQN*WVTEARACDLSTLEANRGGLLEARSSTAA WATKQDLVSTTQK*INAWRHSCSPSYSRG*GKR SA*AQEFNTSVG*DCATVLTPA*AAEQDSISKSK *INKTYKILKIK*NMSGLYLPHLN*GLEELNIL LQMKHFMHRLAHSK*SIGVNNLCYCYFLESN*Q IPQ*MTSQGCNQQDPEYGNFY*INNSISSATIQE RERREAIHFKRLKKYMNQICMRQSEKLTPTVLR NYLILVW**DCCYVF*KVIIL*IFIIK*L*MK

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA* VQQIWRF*PAGCTEKHGCGKVPEVTVFSKSPVT LGQPNTLICLVDNIFPPVVNITWLSNGQSVTEGV SETSFLSKSDHSFFKISYLTFLPSADEIYDCKV EHWGLDQPLLKHWEPEIPAPMSELTETVVCALG LSVGLMGIVVGTVFIIQGLRSVGASRHQGPL*IP SWKGR*D*DWLQLKRQYERKESGRALWT*MWLK VVGELGSGMMMTQEPPRTH*SHVCPVAGASPST GAEEWTC*MT*HYSLARFIISLFSSKYFSSHLFS GT*AAISPQSSQMPLHSFPDLLIFFFFSQMLPT KTCLG*ATRLPNSSVTSI*NLQGSNKFLL*DLC QIFPSFIQG*LKLWLIIGVLLCFNPI*PHFPDHF SCPVTQKPPSTV*PDNMLIS*LTLIFLASLCSH PWHCHPPLNSGNNEINGYRLPLAQNCYSKNFRT KK*VCTNFNVAFKSMTEK*VRIKEI*ISKISKVK MYSQNFKFMKNDDSRSLPLPLSP*DKNSLGRKR NGSQKNIRIRQ*CGCLKRNKYLFLT*G**QWEK GRE*KPQTYLGALNRGAVCLTS*MKLY*ITM*LS LCHSGMKETV**IWLQDVSWKHANIFRNPQPFP LTPPD*GKHELMRETLGRTTQLRQCSSSSGCCV LHWIRHLRGETLTEEMGQQRPHGSKQL*NP*GYF *PCYLHQ*V*ILIGEKIQNINAIIDTV*FGAKN PKSKSRTQYFEASILNFINV*SL*HFWPYFSHP QCRSNNTFLAELLIKFE*F*YLVNALHIVHEHIR KHCGYIYN*FI*KNGSYYMKSTFVFLSLLMI*E IQM*SYG*ISFHMIIEGYFSFSRMREAEIGLVR ELLALEVVIFDFGFQLSKREYIPSLWPISVYSRY IIYNRSLCLFTLGKK*R*FKEKII*NTDWESVS TGNLNQ**S*KCPSQSTDDFQGNKTILFDTTMV KNVIIHLLKSTEYITPRVRLNVNYGLWVIMMCQC KFINYSKCTTQMGDVANGGGYACVGAECIWQQS PAFLAAGTSFMEDNFSTDSWGNGGMIWG*NCET IPPQINKH*LDSHKEHAT*ISCMHS*K*GSHSYE NLMPPLI*RKAGLSW*CWLTHCVVQFLTGHQLV LVHGPGVGDLWDTSAPSAQFFCEPTTALK*GLF LKRKREGNQL*S*MCKIKILV*KTVTLKCTTC*E AFSNRVSNIFHIHFNDS*GKNPAFWSCVHLCVC VCVCVCVCNIRGILNGIMTRVMQKSTNAAQLRL LPQAALWKANLMIAVFY*KDVFKKYLRNVKIAEL KLILKNKSK*FFLSAKNVGPTINRVTDLPA*FK IMQFLNNYLSYLNYFRLHT*SVTSLILNITLFK IYKISGSQRLVLVILALWRPIGEDCLKPGVQQLP GQQSKTLSLQHKNKLMHGGTPVVPATLEAEARG VLEPRSSILQWAKIVPLCSLQPRQQSRTPSLKV NK*IKLTKF*KSNEICQVCTYHI*TRDLKN*TFY YK*STSCTDWHIVSSR*VLTICVIVIFWSPTNK SHSE*HHRDATNKIQNMETSTR*TTPFLQQQFK REREEKLYILKG*RNI*TKFV*GNQKN*HRLY*G II*F*CGNEIAVMFSKKSLYFRFS**NNYE*