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Sequence HLA-DQA1.aAug05
AAGAGCAAAATAATATGAAAAGTATTTAACAAGGAATATAAAGAATCATCAAAGAAAATGTTATAAAACTTCTTTGAGACCACAAGAGATGATAGAGATGGGGTTTCACTGTGTTAGCGAGGATGGTCTTGATCTCCTGACCTCGTGATCCGCCTGCTTCTGCCTCCCAAAGTGCTGGGATTACAGTCGTGAGCCACCGC
GCCTGGCCTCCTCATATTATTTTTTATTGTGCAGTTTATTCAAGAGAGCTTTCCAGCACCTGTGATAAGCCTCAGCCCGCCTGCCGGATAATGAGCCCAC
ATGGATTGAGAGAGGCCTCCTATCCCTGCCAAACCTATTGATGCTACAGAGATGTGAGGGAGCCCACCTGAGAAAAGCTGAACCTGCCCAGTACATAAAA
ACCACTCAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGAT
GAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCTGGCCAGTACACCCATGAA
TTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTG
CACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTT
TTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGG
CAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATG
AGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGAC
TGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACAC
CAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGTGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATC
TAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGG
TACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTT
GATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATAC
CGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAAT
AAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCC
TCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGG
AACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCT
GCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGT
TTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGA
ACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGC
AGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACA
AAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATA
AATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAAT
TTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTA
TATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATAT
TTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGA
GGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATA
GTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGAT
GATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTA
GACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGG
GGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGAT
AGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCT
AGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTG
GCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCT
CAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAA
TTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTG
AGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATG
ACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAA
AGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGT
AGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACAC
ACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGA
GGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGG
CGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGC
TCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTT
GTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGT
GTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAA
ACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCA
AATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTC
ATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA
- HLA-DQA1.F3 (forward primer)
- HLA-DQA1.R3 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
KSKII*KVFNKEYKESSKKML*NFFETTRDDRDGVSLC*RGWS*SPDLVIRLLLPPKVLGLQS*ATA
PGLLILFFIVQFIQESFPAPVISLSPPAG**AH
MD*ERPPIPAKPIDATEM*GSPPEKS*TCPVHK
NHSAQNTNC*GCLGKRMILNKALLLGALALTTVM
SPCGGEDIVADHVASCGVNLYQFYGPSGQYTHE
FDGDEQFYVDLERKETAWRWPEFSKFGGFDPQG
ALRNMAVAKHNLNIMIKRYNSTAATNEVPEVTVF
SKSPVTLGQPNTLICLVDNIFPPVVNITWLSNG
QSVTEGVSETSFLSKSDHSFFKISYLTFLPSAD
EIYDCKVEHWGLDQPLLKHWEPEIPAPMSELTET
VVCALGLSVGLMGIVVGTVFIIQGLRSVGASRH
QGPL*IPSWKGSVTYKDMPGVSHPAT*FLSNLH
LKSPRKQ*IPFMRSMSNFSIFHPGLTETMANNWG
TLMFQSNLTSFPRSFFMSSNTEATKYSIA**YV
DFLADINISCFLVFPPLALPPTPQFRQQ*N*WI
PSALGPELL*QKF*NQKISLY*FQCGF*KYDREI
S*DKGNLNLKNIKSKNVFSKL*IYEE**Q*KPS
SSLVTLR*KFFRQEKKWKSEKH*NKTIMWVSEK
EQILIPHIGLVTMGKGTGVEATDISRSPE*RRSL
PHLLNEAVLDNHVAFPVPLWHEGDSIVNMATGC
FLETCQYLQKSPALSPNPS*LRKA*AYERNPRK
NNTVETM*QQQWVLCPPLDSPSPRRNSHRGNGSA
ATSWL*TAMKSMRIFLTMLPASVSLNFNWRKNT
KH*CNN*YSIVWCKEP*IQIQDSVL*S*YFKLY
KCVKSLTFLALLFSSTM*E**YFPCRVIDKI*II
LIFSQCLTHST*THKKTLWLYL*LIYLKEWIIL
YEKHFCFSQPLNDLGDSNVELWVNFFSYDN*RI
FFFFQNERG*DWIGKRTLSTRSCNI*LWFSTL*E
GIYSLLMAHKCLFKVYHIQQIFMPVYFGEEVKI
VQGENNLKHRLGISKHRKSEPVMIMKMSITEHR
*FSGQ*NYSV*YHNGEKCHYTFAQIHRIYNTKS*
T*CKLWTLGDNDVPM*VHKL*QMYHSDGRCC*W
GRLCMCGSRVYLAAVPSLFGSRDQFYGRQFFHR
*LGEWGYDLGMKL*NYSTSDQQALVRFS*GTCNL
DLLHAQLKIGFALL*ESNATTDLTEGGAQLVML
AHPLRGPVSNRPPTGTGPWPGGWGPLGYLCTFC
SVFL*TYNRFKIRSIFKKEKRR*PIMILNV*NKN
FSMKNSHIKMHNVLRSF*Q*GFKYISYTFQ*FV
RQEPSILELCAFVCVCVCVCVCL*YKGYTEWHN
DQSHAEIYKCCPTQTPSSSSTVESKFNDSSILLK
GCIQKIFT*C*NSRIKTNSKK*E*IVLFIS*KC
RAYYQ*GYRSTCLI*NHAILKQLFELFELFQIT
HIKCDFINT*YHTI*NLQN*WVTEARACDLSTLE
ANRGGLLEARSSTAAWATKQDLVSTTQK*INAW
RHSCSPSYSRG*GKRSA*AQEFNTSVG*DCATV
LTPA*AAEQDSISKSK*INKTYKILKIK*NMSGL
YLPHLN*GLEELNILLQMKHFMHRLAHSK*SIG
VNNLCYCYFLESN*QIPQ*MTSQGCNQQDPEYG
NFY*INNSISSATIQERERREAIHFKRLKKYMNQ
ICMRQSEKLTPTVLRNYLILVW**DCCYVF*KV
IIL*IFIIK*L*MK
Frame 1
RAK*YEKYLTRNIKNHQRKCYKTSLRPQEMIEMGFHCVSEDGLDLLTS*SACFCLPKCWDYSREPP
RLASSYYFLLCSLFKRAFQHL**ASARLPDNEPT
WIERGLLSLPNLLMLQRCEGAHLRKAEPAQYIK
TTQLRTPTAEAALGRG*S*TKLCCWGPSL*PP*
*APVEVKTLWLTTLPLVV*TCTSFTVPLASTPMN
LMEMSSSTWTWRGRRLPGGGLSSANLEVLTRRV
H*ETWLWQNTT*TS*LNATTLPLLPMRFLRSQC
FPSLP*HWVSPTPSFVLWTTSFLLWSTSHG*AMG
SQSQKVFLRPASSPRVIIPSSRSVTSPSSLLLM
RFMTARWSTGAWTSLF*NTGSLRFQPLCQSSQR
LWSVPWGCLWASWALWWALSSSSKACVQLVLPDT
KGHCESHPGREVLPTKTCLG*ATRLPNSSVTSI
*NLQGSNKFLL*DLCQIFPSFIQG*LKLWLIIG
VLLCFNPI*PHFPDHFSCPVTQKPPSTV*PDNML
IS*LTLIFLASLCSHPWHCHPPLNSGNNEINGY
RLPLAQNCYSKNFRTKK*VCTNFNVAFKSMTEK
*VRIKEI*ISKISKVKMYSQNFKFMKNDDSRSLP
LPLSP*DKNSLGRKRNGSQKNIRIRQ*CGCLKR
NKYLFLT*G**QWEKGRE*KPQTYLGALNRGAV
CLTS*MKLY*ITM*LSLCHSGMKETV**IWLQDV
SWKHANIFRNPQPFPLTPPD*GKHELMRETLGR
TTQLRQCSSSSGCCVLHWIRHLRGETLTEEMGQ
QRPHGSKQL*NP*GYF*PCYLHQ*V*ILIGEKIQ
NINAIIDTV*FGAKNPKSKSRTQYFEASILNFI
NV*SL*HFWPYFSHPQCRSNNTFLAELLIKFE*
F*YLVNALHIVHEHIRKHCGYIYN*FI*KNGSYY
MKSTFVFLSLLMI*EIQM*SYG*ISFHMIIEGY
FSFSRMREAEIGLVRELLALEVVIFDFGFQLSK
REYIPSLWPISVYSRYIIYNRSLCLFTLGKK*R*
FKEKII*NTDWESVSTGNLNQ**S*KCPSQSTD
DFQGNKTILFDTTMVKNVIIHLLKSTEYITPRV
RLNVNYGLWVIMMCQCKFINYSKCTTQMGDVANG
GGYACVGAECIWQQSPAFLAAGTSFMEDNFSTD
SWGNGGMIWG*NCETIPPQINKH*LDSHKEHAT
*ISCMHS*K*GSHSYENLMPPLI*RKAGLSW*CW
LTHCVVQFLTGHQLVLVHGPGVGDLWDTSAPSA
QFFCEPTTALK*GLFLKRKREGNQL*S*MCKIK
ILV*KTVTLKCTTC*EAFSNRVSNIFHIHFNDS*
GKNPAFWSCVHLCVCVCVCVCVCNIRGILNGIM
TRVMQKSTNAAQLRLLPQAALWKANLMIAVFY*
KDVFKKYLRNVKIAELKLILKNKSK*FFLSAKNV
GPTINRVTDLPA*FKIMQFLNNYLSYLNYFRLH
T*SVTSLILNITLFKIYKISGSQRLVLVILALW
RPIGEDCLKPGVQQLPGQQSKTLSLQHKNKLMHG
GTPVVPATLEAEARGVLEPRSSILQWAKIVPLC
SLQPRQQSRTPSLKVNK*IKLTKF*KSNEICQV
CTYHI*TRDLKN*TFYYK*STSCTDWHIVSSR*V
LTICVIVIFWSPTNKSHSE*HHRDATNKIQNME
TSTR*TTPFLQQQFKREREEKLYILKG*RNI*T
KFV*GNQKN*HRLY*GII*F*CGNEIAVMFSKKS
LYFRFS**NNYE*
Frame 2
EQNNMKSI*QGI*RIIKENVIKLL*DHKR**RWGFTVLARMVLIS*PRDPPASASQSAGITVVSHR
AWPPHIIFYCAVYSRELSSTCDKPQPACRIMSP
HGLREASYPCQTY*CYRDVREPT*EKLNLPST*K
PLSSEHQLLRLPWEEDDPKQSSAAGGPRSDHRD
EPLWR*RHCG*PRCLLWCKLVPVLRSLWPVHP*
I*WR*AVLRGPGEEGDCLAVA*VQQIWRF*PAGC
TEKHGCGKTQLEHHD*TLQLYRCYQ*GS*GHSV
FQVSRDTGSAQHPHLSCGQHLSSCGQHHMAEQW
AVSHRRCF*DQLPLQE*SFLLQDQLPHLPPFC**
DL*LQGGALGPGPASSETLGA*DSSPYVRAHRD
CGLCPGVVCGPHGHCGGHCLHHPRPAFSWCFQT
PRAIVNPILEGKCYLQRHAWGKPPGYLIPQ*PPS
KISKEAINSFYEIYVKFFHLSSRAD*NYG**LG
YSYVSIQFNLISQIIFHVQ*HRSHQVQYSLIIC
*FLS*H*YFLLPCVPTLGTATHPSIQATMKLMDT
VCPWPRIVIAKILEPKNKSVLISMWLLKV*QRN
KLG*RKFESQKYQK*KCILKTLNL*RMMTVEAF
LFPCHLEIKIL*AGKEMEVRKTLE*DNNVGV*KG
TNTYSSHRVSDNGKRDGSRSHRHI*EP*IEAQS
ASPPE*SCTR*PCSFPCATLA*RRQYSEYGYRM
FLGNMPISSEIPSPFP*PLLTKESMSL*EKP*EE
QHS*DNVAAAVGAVSSTGFAISEEKLSQRKWVS
SDLMALNSYEIHEDISNHATCISEFKF*LEKKY
KTLMQ*LIQYSLVQRTLNPNPGLSTLKLVF*TL*
MCEVSNISGLTFLIHNVGVIILSLQSY**NLNN
FDI*SMPYT*YMNT*ENIVVIFIINLFKRMDHI
I*KALLFFSAS**FRRFKCRAMGEFLFI**LKDI
FLFPE*ERLRLDW*ENS*H*KL*YLTLVFNSLR
GNIFPPYGP*VFIQGISYTTDLYACLLWGRSED
SSRRK*FKTQTGNQ*AQEI*TSDDHENVHHRAQM
IFRAIKLFCLIPQW*KMSLYICSNPQNI*HQEL
DLM*TMDFG***CANVSS*IIANVPLRWEMLLM
GEAMHVWEQSVSGSSPQPFWQQGPVLWKTIFPQI
VGGMGV*SGDETVKLFHLRSTSIS*ILIRNMQP
RSLACTVKNRVRTPMRI*CHH*SDGRRGSVGNA
GSPTAWSSF*QATNWYWSMARGLGTSGIPLHLLL
SFSVNLQPL*NKVYF*KGKEKVTNYDPKCVK*K
F*YEKQSH*NAQRAKKLLAIGFQIYFIYISMIR
EARTQHFGVVCICVCVCVCVCVSVI*GVY*MA**
PESCRNLQMLPNSDSFLKQHCGKQI***QYFIK
RMYSKNIYVMLK*QN*N*F*KIRVNSSFYQLKM
*GLLSIGLQIYLPDLKSCNS*TII*AI*IISDYT
HKV*LH*YLISHYLKFTKLVGHRGSCL*S*HFG
GQ*GRIA*SQEFNSCLGNKARPCLYNTKIN*CM
AALL*SQLL*RLRQEECLSPGVQYFSGLRLCHCA
HSSLGSRAGLHL*K*INK*NLQNFKNQMKYVRF
VPTTFKLGT*RIKHFITNEALHAQTGT**VVDR
C*QFVLLLFSGVQLTNPTVNDITGMQPTRSRIWK
LLLDKQLHFFSNNSREREKRSYTF*KAEEIYEP
NLYEAIRKTDTDCIKELSNFSVVMRLLLCFLKS
HYTLDFHNKIIMNE
Sequence HLA-DQA1.fAug05
GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCCAGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG
TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT
CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC
AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA
GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC
GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC
CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA
GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGGTGCA
TCGCCATCTACAGGAGCAGAAGAATGGACTTGCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCT
CTCACCTTTTCTCTGGGACTTAAGCTGCTATATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCA
AATGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTA
TGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATT
TCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTC
CTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAA
AATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAA
TATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTT
AGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGA
CAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAA
CCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCC
CCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGT
GGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATC
CATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTT
TGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTAC
TTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTAC
ATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTT
AATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATT
GGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTG
TTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTG
GGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGAT
ACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAA
TGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTAT
CTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGA
AACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTT
CGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGG
CCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAA
TAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCA
CAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCA
TTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCC
CAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTA
AAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACC
TGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATC
ACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAG
TTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGC
TGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCA
TCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGA
ATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAG
TCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAG
CAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACC
GACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTA
TGAATGAAA
- HLA-DQA1.F3 (forward primer)
- HLA-DQA1.R3 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSFQGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC
LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA
LLLGALALTTVMSPCGGEDIVADHVASCGVNLY
QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE
FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST
AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF
PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK
ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWGA
SPSTGAEEWTC*MT*HYSLARFIISLFSSKYFS
SHLFSGT*AAISPQSSQMPLHSFPDLLIFFFFSQ
MLPTKTCLG*ATRLPNSSVTSI*NLQGSNKFLL
*DLCQIFPSFIQG*LKLWLIIGVLLCFNPI*PH
FPDHFSCPVTQKPPSTV*PDNMLIS*LTLIFLAS
LCSHPWHCHPPLNSGNNEINGYRLPLAQNCYSK
NFRTKK*VCTNFNVAFKSMTEK*VRIKEI*ISK
ISKVKMYSQNFKFMKNDDSRSLPLPLSP*DKNSL
GRKRNGSQKNIRIRQ*CGCLKRNKYLFLT*G**
QWEKGRE*KPQTYLGALNRGAVCLTS*MKLY*I
TM*LSLCHSGMKETV**IWLQDVSWKHANIFRNP
QPFPLTPPD*GKHELMRETLGRTTQLRQCSSSS
GCCVLHWIRHLRGETLTEEMGQQRPHGSKQL*N
P*GYF*PCYLHQ*V*ILIGEKIQNINAIIDTV*F
GAKNPKSKSRTQYFEASILNFINV*SL*HFWPY
FSHPQCRSNNTFLAELLIKFE*F*YLVNALHIV
HEHIRKHCGYIYN*FI*KNGSYYMKSTFVFLSLL
MI*EIQM*SYG*ISFHMIIEGYFSFSRMREAEI
GLVRELLALEVVIFDFGFQLSKREYIPSLWPIS
VYSRYIIYNRSLCLFTLGKK*R*FKEKII*NTDW
ESVSTGNLNQ**S*KCPSQSTDDFQGNKTILFD
TTMVKNVIIHLLKSTEYITPRVRLNVNYGLWVI
MMCQCKFINYSKCTTQMGDVANGGGYACVGAECI
WQQSPAFLAAGTSFMEDNFSTDSWGNGGMIWG*
NCETIPPQINKH*LDSHKEHAT*ISCMHS*K*G
SHSYENLMPPLI*RKAGLSW*CWLTHCVVQFLTG
HQLVLVHGPGVGDLWDTSAPSAQFFCEPTTALK
*GLFLKRKREGNQL*S*MCKIKILV*KTVTLKC
TTC*EAFSNRVSNIFHIHFNDS*GKNPAFWSCVH
LCVCVCVCVCVCNIRGILNGIMTRVMQKSTNAA
QLRLLPQAALWKANLMIAVFY*KDVFKKYLRNV
KIAELKLILKNKSK*FFLSAKNVGPTINRVTDLP
A*FKIMQFLNNYLSYLNYFRLHT*SVTSLILNI
TLFKIYKISGSQRLVLVILALWRPIGEDCLKPG
VQQLPGQQSKTLSLQHKNKLMHGGTPVVPATLEA
EARGVLEPRSSILQWAKIVPLCSLQPRQQSRTP
SLKVNK*IKLTKF*KSNEICQVCTYHI*TRDLK
N*TFYYK*STSCTDWHIVSSR*VLTICVIVIFWS
PTNKSHSE*HHRDATNKIQNMETSTR*TTPFLQ
QQFKREREEKLYILKG*RNI*TKFV*GNQKN*H
RLY*GII*F*CGNEIAVMFSKKSLYFRFS**NNY
E*
Frame 1
KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPSRALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG
VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL
CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT
SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL
SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP
LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF
LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS
RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGVH
RHLQEQKNGLAK*PSTILWPDLSYPFSPPNISP
LTFSLGLKLLYPLRAHKCLYILSLTS*FFFSFL
KCYLQRHAWGKPPGYLIPQ*PPSKISKEAINSFY
EIYVKFFHLSSRAD*NYG**LGYSYVSIQFNLI
SQIIFHVQ*HRSHQVQYSLIIC*FLS*H*YFLL
PCVPTLGTATHPSIQATMKLMDTVCPWPRIVIAK
ILEPKNKSVLISMWLLKV*QRNKLG*RKFESQK
YQK*KCILKTLNL*RMMTVEAFLFPCHLEIKIL
*AGKEMEVRKTLE*DNNVGV*KGTNTYSSHRVSD
NGKRDGSRSHRHI*EP*IEAQSASPPE*SCTR*
PCSFPCATLA*RRQYSEYGYRMFLGNMPISSEI
PSPFP*PLLTKESMSL*EKP*EEQHS*DNVAAAV
GAVSSTGFAISEEKLSQRKWVSSDLMALNSYEI
HEDISNHATCISEFKF*LEKKYKTLMQ*LIQYS
LVQRTLNPNPGLSTLKLVF*TL*MCEVSNISGLT
FLIHNVGVIILSLQSY**NLNNFDI*SMPYT*Y
MNT*ENIVVIFIINLFKRMDHII*KALLFFSAS
**FRRFKCRAMGEFLFI**LKDIFLFPE*ERLRL
DW*ENS*H*KL*YLTLVFNSLRGNIFPPYGP*V
FIQGISYTTDLYACLLWGRSEDSSRRK*FKTQT
GNQ*AQEI*TSDDHENVHHRAQMIFRAIKLFCLI
PQW*KMSLYICSNPQNI*HQELDLM*TMDFG**
*CANVSS*IIANVPLRWEMLLMGEAMHVWEQSV
SGSSPQPFWQQGPVLWKTIFPQIVGGMGV*SGDE
TVKLFHLRSTSIS*ILIRNMQPRSLACTVKNRV
RTPMRI*CHH*SDGRRGSVGNAGSPTAWSSF*Q
ATNWYWSMARGLGTSGIPLHLLLSFSVNLQPL*N
KVYF*KGKEKVTNYDPKCVK*KF*YEKQSH*NA
QRAKKLLAIGFQIYFIYISMIREARTQHFGVVC
ICVCVCVCVCVSVI*GVY*MA**PESCRNLQMLP
NSDSFLKQHCGKQI***QYFIKRMYSKNIYVML
K*QN*N*F*KIRVNSSFYQLKM*GLLSIGLQIY
LPDLKSCNS*TII*AI*IISDYTHKV*LH*YLIS
HYLKFTKLVGHRGSCL*S*HFGGQ*GRIA*SQE
FNSCLGNKARPCLYNTKIN*CMAALL*SQLL*R
LRQEECLSPGVQYFSGLRLCHCAHSSLGSRAGLH
L*K*INK*NLQNFKNQMKYVRFVPTTFKLGT*R
IKHFITNEALHAQTGT**VVDRC*QFVLLLFSG
VQLTNPTVNDITGMQPTRSRIWKLLLDKQLHFFS
NNSREREKRSYTF*KAEEIYEPNLYEAIRKTDT
DCIKELSNFSVVMRLLLCFLKSHYTLDFHNKII
MNE
Frame 2
RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLPGLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV
SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS
SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP
VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*
VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY
RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS
SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ
DQLPHLPPFC**DL*LQGGALGPGPASSETLGC
IAIYRSRRMDLLNDLALFSGPIYHIPFLLQIFLL
SPFLWDLSCYIPSELTNAFTFFP*PPDFFFLFS
NVTYKDMPGVSHPAT*FLSNLHLKSPRKQ*IPF
MRSMSNFSIFHPGLTETMANNWGTLMFQSNLTSF
PRSFFMSSNTEATKYSIA**YVDFLADINISCF
LVFPPLALPPTPQFRQQ*N*WIPSALGPELL*Q
KF*NQKISLY*FQCGF*KYDREIS*DKGNLNLKN
IKSKNVFSKL*IYEE**Q*KPSSSLVTLR*KFF
RQEKKWKSEKH*NKTIMWVSEKEQILIPHIGLV
TMGKGTGVEATDISRSPE*RRSLPHLLNEAVLDN
HVAFPVPLWHEGDSIVNMATGCFLETCQYLQKS
PALSPNPS*LRKA*AYERNPRKNNTVETM*QQQ
WVLCPPLDSPSPRRNSHRGNGSAATSWL*TAMKS
MRIFLTMLPASVSLNFNWRKNTKH*CNN*YSIV
WCKEP*IQIQDSVL*S*YFKLYKCVKSLTFLAL
LFSSTM*E**YFPCRVIDKI*IILIFSQCLTHST
*THKKTLWLYL*LIYLKEWIILYEKHFCFSQPL
NDLGDSNVELWVNFFSYDN*RIFFFFQNERG*D
WIGKRTLSTRSCNI*LWFSTL*EGIYSLLMAHKC
LFKVYHIQQIFMPVYFGEEVKIVQGENNLKHRL
GISKHRKSEPVMIMKMSITEHR*FSGQ*NYSV*
YHNGEKCHYTFAQIHRIYNTKS*T*CKLWTLGDN
DVPM*VHKL*QMYHSDGRCC*WGRLCMCGSRVY
LAAVPSLFGSRDQFYGRQFFHR*LGEWGYDLGM
KL*NYSTSDQQALVRFS*GTCNLDLLHAQLKIGF
ALL*ESNATTDLTEGGAQLVMLAHPLRGPVSNR
PPTGTGPWPGGWGPLGYLCTFCSVFL*TYNRFK
IRSIFKKEKRR*PIMILNV*NKNFSMKNSHIKMH
NVLRSF*Q*GFKYISYTFQ*FVRQEPSILELCA
FVCVCVCVCVCL*YKGYTEWHNDQSHAEIYKCC
PTQTPSSSSTVESKFNDSSILLKGCIQKIFT*C*
NSRIKTNSKK*E*IVLFIS*KCRAYYQ*GYRST
CLI*NHAILKQLFELFELFQITHIKCDFINT*Y
HTI*NLQN*WVTEARACDLSTLEANRGGLLEARS
STAAWATKQDLVSTTQK*INAWRHSCSPSYSRG
*GKRSA*AQEFNTSVG*DCATVLTPA*AAEQDS
ISKSK*INKTYKILKIK*NMSGLYLPHLN*GLEE
LNILLQMKHFMHRLAHSK*SIGVNNLCYCYFLE
SN*QIPQ*MTSQGCNQQDPEYGNFY*INNSISS
ATIQERERREAIHFKRLKKYMNQICMRQSEKLTP
TVLRNYLILVW**DCCYVF*KVIIL*IFIIK*L
*MK
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