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Sequences

Sequence HLA-DQA1.aAug05

AAGAGCAAAATAATATGAAAAGTATTTAACAAGGAATATAAAGAATCATCAAAGAAAATGTTATAAAACTTCTTTGAGACCACAAGAGATGATAGAGATG
GGGTTTCACTGTGTTAGCGAGGATGGTCTTGATCTCCTGACCTCGTGATCCGCCTGCTTCTGCCTCCCAAAGTGCTGGGATTACAGTCGTGAGCCACCGC
GCCTGGCCTCCTCATATTATTTTTTATTGTGCAGTTTATTCAAGAGAGCTTTCCAGCACCTGTGATAAGCCTCAGCCCGCCTGCCGGATAATGAGCCCAC
ATGGATTGAGAGAGGCCTCCTATCCCTGCCAAACCTATTGATGCTACAGAGATGTGAGGGAGCCCACCTGAGAAAAGCTGAACCTGCCCAGTACATAAAA
ACCACTCAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGAT
GAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCTGGCCAGTACACCCATGAA
TTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTG
CACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTT
TTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGG
CAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATG
AGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGAC
TGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACAC
CAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGTGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATC
TAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGG
TACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTT
GATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATAC
CGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAAT
AAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCC
TCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGG
AACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCT
GCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGT
TTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGA
ACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGC
AGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACA
AAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATA
AATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAAT
TTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTA
TATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATAT
TTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGA
GGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATA
GTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGAT
GATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTA
GACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGG
GGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGAT
AGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCT
AGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTG
GCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCT
CAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAA
TTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTG
AGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATG
ACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAA
AGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGT
AGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACAC
ACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGA
GGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGG
CGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGC
TCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTT
GTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGT
GTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAA
ACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCA
AATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTC
ATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA
  • HLA-DQA1.F3 (forward primer)
  • HLA-DQA1.R3 (reverse primer)
  • Alternative donor and acceptor

Frame 0

KSKII*KVFNKEYKESSKKML*NFFETTRDDRD
GVSLC*RGWS*SPDLVIRLLLPPKVLGLQS*ATA
PGLLILFFIVQFIQESFPAPVISLSPPAG**AH
MD*ERPPIPAKPIDATEM*GSPPEKS*TCPVHK
NHSAQNTNC*GCLGKRMILNKALLLGALALTTVM
SPCGGEDIVADHVASCGVNLYQFYGPSGQYTHE
FDGDEQFYVDLERKETAWRWPEFSKFGGFDPQG
ALRNMAVAKHNLNIMIKRYNSTAATNEVPEVTVF
SKSPVTLGQPNTLICLVDNIFPPVVNITWLSNG
QSVTEGVSETSFLSKSDHSFFKISYLTFLPSAD
EIYDCKVEHWGLDQPLLKHWEPEIPAPMSELTET
VVCALGLSVGLMGIVVGTVFIIQGLRSVGASRH
QGPL*IPSWKGSVTYKDMPGVSHPAT*FLSNLH
LKSPRKQ*IPFMRSMSNFSIFHPGLTETMANNWG
TLMFQSNLTSFPRSFFMSSNTEATKYSIA**YV
DFLADINISCFLVFPPLALPPTPQFRQQ*N*WI
PSALGPELL*QKF*NQKISLY*FQCGF*KYDREI
S*DKGNLNLKNIKSKNVFSKL*IYEE**Q*KPS
SSLVTLR*KFFRQEKKWKSEKH*NKTIMWVSEK
EQILIPHIGLVTMGKGTGVEATDISRSPE*RRSL
PHLLNEAVLDNHVAFPVPLWHEGDSIVNMATGC
FLETCQYLQKSPALSPNPS*LRKA*AYERNPRK
NNTVETM*QQQWVLCPPLDSPSPRRNSHRGNGSA
ATSWL*TAMKSMRIFLTMLPASVSLNFNWRKNT
KH*CNN*YSIVWCKEP*IQIQDSVL*S*YFKLY
KCVKSLTFLALLFSSTM*E**YFPCRVIDKI*II
LIFSQCLTHST*THKKTLWLYL*LIYLKEWIIL
YEKHFCFSQPLNDLGDSNVELWVNFFSYDN*RI
FFFFQNERG*DWIGKRTLSTRSCNI*LWFSTL*E
GIYSLLMAHKCLFKVYHIQQIFMPVYFGEEVKI
VQGENNLKHRLGISKHRKSEPVMIMKMSITEHR
*FSGQ*NYSV*YHNGEKCHYTFAQIHRIYNTKS*
T*CKLWTLGDNDVPM*VHKL*QMYHSDGRCC*W
GRLCMCGSRVYLAAVPSLFGSRDQFYGRQFFHR
*LGEWGYDLGMKL*NYSTSDQQALVRFS*GTCNL
DLLHAQLKIGFALL*ESNATTDLTEGGAQLVML
AHPLRGPVSNRPPTGTGPWPGGWGPLGYLCTFC
SVFL*TYNRFKIRSIFKKEKRR*PIMILNV*NKN
FSMKNSHIKMHNVLRSF*Q*GFKYISYTFQ*FV
RQEPSILELCAFVCVCVCVCVCL*YKGYTEWHN
DQSHAEIYKCCPTQTPSSSSTVESKFNDSSILLK
GCIQKIFT*C*NSRIKTNSKK*E*IVLFIS*KC
RAYYQ*GYRSTCLI*NHAILKQLFELFELFQIT
HIKCDFINT*YHTI*NLQN*WVTEARACDLSTLE
ANRGGLLEARSSTAAWATKQDLVSTTQK*INAW
RHSCSPSYSRG*GKRSA*AQEFNTSVG*DCATV
LTPA*AAEQDSISKSK*INKTYKILKIK*NMSGL
YLPHLN*GLEELNILLQMKHFMHRLAHSK*SIG
VNNLCYCYFLESN*QIPQ*MTSQGCNQQDPEYG
NFY*INNSISSATIQERERREAIHFKRLKKYMNQ
ICMRQSEKLTPTVLRNYLILVW**DCCYVF*KV
IIL*IFIIK*L*MK

Frame 1

RAK*YEKYLTRNIKNHQRKCYKTSLRPQEMIEM
GFHCVSEDGLDLLTS*SACFCLPKCWDYSREPP
RLASSYYFLLCSLFKRAFQHL**ASARLPDNEPT
WIERGLLSLPNLLMLQRCEGAHLRKAEPAQYIK
TTQLRTPTAEAALGRG*S*TKLCCWGPSL*PP*
*APVEVKTLWLTTLPLVV*TCTSFTVPLASTPMN
LMEMSSSTWTWRGRRLPGGGLSSANLEVLTRRV
H*ETWLWQNTT*TS*LNATTLPLLPMRFLRSQC
FPSLP*HWVSPTPSFVLWTTSFLLWSTSHG*AMG
SQSQKVFLRPASSPRVIIPSSRSVTSPSSLLLM
RFMTARWSTGAWTSLF*NTGSLRFQPLCQSSQR
LWSVPWGCLWASWALWWALSSSSKACVQLVLPDT
KGHCESHPGREVLPTKTCLG*ATRLPNSSVTSI
*NLQGSNKFLL*DLCQIFPSFIQG*LKLWLIIG
VLLCFNPI*PHFPDHFSCPVTQKPPSTV*PDNML
IS*LTLIFLASLCSHPWHCHPPLNSGNNEINGY
RLPLAQNCYSKNFRTKK*VCTNFNVAFKSMTEK
*VRIKEI*ISKISKVKMYSQNFKFMKNDDSRSLP
LPLSP*DKNSLGRKRNGSQKNIRIRQ*CGCLKR
NKYLFLT*G**QWEKGRE*KPQTYLGALNRGAV
CLTS*MKLY*ITM*LSLCHSGMKETV**IWLQDV
SWKHANIFRNPQPFPLTPPD*GKHELMRETLGR
TTQLRQCSSSSGCCVLHWIRHLRGETLTEEMGQ
QRPHGSKQL*NP*GYF*PCYLHQ*V*ILIGEKIQ
NINAIIDTV*FGAKNPKSKSRTQYFEASILNFI
NV*SL*HFWPYFSHPQCRSNNTFLAELLIKFE*
F*YLVNALHIVHEHIRKHCGYIYN*FI*KNGSYY
MKSTFVFLSLLMI*EIQM*SYG*ISFHMIIEGY
FSFSRMREAEIGLVRELLALEVVIFDFGFQLSK
REYIPSLWPISVYSRYIIYNRSLCLFTLGKK*R*
FKEKII*NTDWESVSTGNLNQ**S*KCPSQSTD
DFQGNKTILFDTTMVKNVIIHLLKSTEYITPRV
RLNVNYGLWVIMMCQCKFINYSKCTTQMGDVANG
GGYACVGAECIWQQSPAFLAAGTSFMEDNFSTD
SWGNGGMIWG*NCETIPPQINKH*LDSHKEHAT
*ISCMHS*K*GSHSYENLMPPLI*RKAGLSW*CW
LTHCVVQFLTGHQLVLVHGPGVGDLWDTSAPSA
QFFCEPTTALK*GLFLKRKREGNQL*S*MCKIK
ILV*KTVTLKCTTC*EAFSNRVSNIFHIHFNDS*
GKNPAFWSCVHLCVCVCVCVCVCNIRGILNGIM
TRVMQKSTNAAQLRLLPQAALWKANLMIAVFY*
KDVFKKYLRNVKIAELKLILKNKSK*FFLSAKNV
GPTINRVTDLPA*FKIMQFLNNYLSYLNYFRLH
T*SVTSLILNITLFKIYKISGSQRLVLVILALW
RPIGEDCLKPGVQQLPGQQSKTLSLQHKNKLMHG
GTPVVPATLEAEARGVLEPRSSILQWAKIVPLC
SLQPRQQSRTPSLKVNK*IKLTKF*KSNEICQV
CTYHI*TRDLKN*TFYYK*STSCTDWHIVSSR*V
LTICVIVIFWSPTNKSHSE*HHRDATNKIQNME
TSTR*TTPFLQQQFKREREEKLYILKG*RNI*T
KFV*GNQKN*HRLY*GII*F*CGNEIAVMFSKKS
LYFRFS**NNYE*

Frame 2

EQNNMKSI*QGI*RIIKENVIKLL*DHKR**RW
GFTVLARMVLIS*PRDPPASASQSAGITVVSHR
AWPPHIIFYCAVYSRELSSTCDKPQPACRIMSP
HGLREASYPCQTY*CYRDVREPT*EKLNLPST*K
PLSSEHQLLRLPWEEDDPKQSSAAGGPRSDHRD
EPLWR*RHCG*PRCLLWCKLVPVLRSLWPVHP*
I*WR*AVLRGPGEEGDCLAVA*VQQIWRF*PAGC
TEKHGCGKTQLEHHD*TLQLYRCYQ*GS*GHSV
FQVSRDTGSAQHPHLSCGQHLSSCGQHHMAEQW
AVSHRRCF*DQLPLQE*SFLLQDQLPHLPPFC**
DL*LQGGALGPGPASSETLGA*DSSPYVRAHRD
CGLCPGVVCGPHGHCGGHCLHHPRPAFSWCFQT
PRAIVNPILEGKCYLQRHAWGKPPGYLIPQ*PPS
KISKEAINSFYEIYVKFFHLSSRAD*NYG**LG
YSYVSIQFNLISQIIFHVQ*HRSHQVQYSLIIC
*FLS*H*YFLLPCVPTLGTATHPSIQATMKLMDT
VCPWPRIVIAKILEPKNKSVLISMWLLKV*QRN
KLG*RKFESQKYQK*KCILKTLNL*RMMTVEAF
LFPCHLEIKIL*AGKEMEVRKTLE*DNNVGV*KG
TNTYSSHRVSDNGKRDGSRSHRHI*EP*IEAQS
ASPPE*SCTR*PCSFPCATLA*RRQYSEYGYRM
FLGNMPISSEIPSPFP*PLLTKESMSL*EKP*EE
QHS*DNVAAAVGAVSSTGFAISEEKLSQRKWVS
SDLMALNSYEIHEDISNHATCISEFKF*LEKKY
KTLMQ*LIQYSLVQRTLNPNPGLSTLKLVF*TL*
MCEVSNISGLTFLIHNVGVIILSLQSY**NLNN
FDI*SMPYT*YMNT*ENIVVIFIINLFKRMDHI
I*KALLFFSAS**FRRFKCRAMGEFLFI**LKDI
FLFPE*ERLRLDW*ENS*H*KL*YLTLVFNSLR
GNIFPPYGP*VFIQGISYTTDLYACLLWGRSED
SSRRK*FKTQTGNQ*AQEI*TSDDHENVHHRAQM
IFRAIKLFCLIPQW*KMSLYICSNPQNI*HQEL
DLM*TMDFG***CANVSS*IIANVPLRWEMLLM
GEAMHVWEQSVSGSSPQPFWQQGPVLWKTIFPQI
VGGMGV*SGDETVKLFHLRSTSIS*ILIRNMQP
RSLACTVKNRVRTPMRI*CHH*SDGRRGSVGNA
GSPTAWSSF*QATNWYWSMARGLGTSGIPLHLLL
SFSVNLQPL*NKVYF*KGKEKVTNYDPKCVK*K
F*YEKQSH*NAQRAKKLLAIGFQIYFIYISMIR
EARTQHFGVVCICVCVCVCVCVSVI*GVY*MA**
PESCRNLQMLPNSDSFLKQHCGKQI***QYFIK
RMYSKNIYVMLK*QN*N*F*KIRVNSSFYQLKM
*GLLSIGLQIYLPDLKSCNS*TII*AI*IISDYT
HKV*LH*YLISHYLKFTKLVGHRGSCL*S*HFG
GQ*GRIA*SQEFNSCLGNKARPCLYNTKIN*CM
AALL*SQLL*RLRQEECLSPGVQYFSGLRLCHCA
HSSLGSRAGLHL*K*INK*NLQNFKNQMKYVRF
VPTTFKLGT*RIKHFITNEALHAQTGT**VVDR
C*QFVLLLFSGVQLTNPTVNDITGMQPTRSRIWK
LLLDKQLHFFSNNSREREKRSYTF*KAEEIYEP
NLYEAIRKTDTDCIKELSNFSVVMRLLLCFLKS
HYTLDFHNKIIMNE

Sequence HLA-DQA1.jAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC
AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG
TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT
CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC
AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA
GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC
GCTGCTACCAATGGTATGCGTCCACCATTCTGCCTCTCTTTACTTAAGTTATCCCTCCATACCAGGGTTCATTATTTTCTTCCCAAGAGGTCCCCAGATC
TTCTTATGGCAATTGCTGAAATTTTATCATCTCCCATCTCTAAAATCACATATTCCCATGTAATACAAGGGTCTTTCCATTATGCATTCAGCAAATCCTT
CTAGGAGAGGTCTCATCAACCTCCTACTTTATTAAACATGCCCACAGAGAGAAGGGCACAGGAATAAAGCAGAGGCAATGTGTCGTTGCTCCCAAGCAGA
AGGTAAATAAGACCTCTTTGACTATCAGGTGGTGAAATGCTGGTAGGAGGGCTCTTCCAGGATGTAATGCAGAACTTCAGGGCAGAGCTATTCACACTTC
ACACCAGTGCTGTTTCCTCACCACAGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTG
GACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATC
ATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAA
ACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGC
ACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAGTGCATCGCCATCTACAGGAGCAGAAGAATGGACTTG
CTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGCTGCTATA
TCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTGGGGTAAG
CCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCAT
CCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAG
AAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCAC
CCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAA
TTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTT
AAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACA
TTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACA
GACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATG
AAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAG
GAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCT
CCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCAT
CAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGA
CTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATAC
TTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATT
TATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATG
GGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGT
TGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCT
TTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGT
GATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTT
GCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGC
AAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAG
GGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAA
GCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTG
ATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGG
TTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGG
TAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTT
TCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTG
GAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAAT
AAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACA
ATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTC
ACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACC
TTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTT
CAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTAC
AAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCA
TGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATC
ACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCT
ATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGT
GGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA
  • HLA-DQA1.F3 (forward primer)
  • HLA-DQA1.R3 (reverse primer)
  • Alternative donor and acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF
QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC
LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA
LLLGALALTTVMSPCGGEDIVADHVASCGVNLY
QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE
FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST
AATNGMRPPFCLSLLKLSLHTRVHYFLPKRSPD
LLMAIAEILSSPISKITYSHVIQGSFHYAFSKSF
*ERSHQPPTLLNMPTERRAQE*SRGNVSLLPSR
R*IRPL*LSGGEMLVGGLFQDVMQNFRAELFTL
HTSAVSSPQRFLRSQCFPSLP*HWVSPTPSFVLW
TTSFLLWSTSHG*AMGSQSQKVFLRPASSPRVI
IPSSRSVTSPSSLLLMRFMTARWSTGAWTSLF*
NTGSLRFQPLCQSSQRLWSVPWGCLWASWALWWA
LSSSSKACVQLVLPDTKGHCECIAIYRSRRMDL
LNDLALFSGPIYHIPFLLQIFLLSPFLWDLSCY
IPSELTNAFTFFP*PPDFFFLFSNVTYKDMPGVS
HPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSIFH
PGLTETMANNWGTLMFQSNLTSFPRSFFMSSNT
EATKYSIA**YVDFLADINISCFLVFPPLALPPT
PQFRQQ*N*WIPSALGPELL*QKF*NQKISLY*
FQCGF*KYDREIS*DKGNLNLKNIKSKNVFSKL
*IYEE**Q*KPSSSLVTLR*KFFRQEKKWKSEKH
*NKTIMWVSEKEQILIPHIGLVTMGKGTGVEAT
DISRSPE*RRSLPHLLNEAVLDNHVAFPVPLWH
EGDSIVNMATGCFLETCQYLQKSPALSPNPS*LR
KA*AYERNPRKNNTVETM*QQQWVLCPPLDSPS
PRRNSHRGNGSAATSWL*TAMKSMRIFLTMLPA
SVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQIQD
SVL*S*YFKLYKCVKSLTFLALLFSSTM*E**Y
FPCRVIDKI*IILIFSQCLTHST*THKKTLWLY
L*LIYLKEWIILYEKHFCFSQPLNDLGDSNVELW
VNFFSYDN*RIFFFFQNERG*DWIGKRTLSTRS
CNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQQI
FMPVYFGEEVKIVQGENNLKHRLGISKHRKSEPV
MIMKMSITEHR*FSGQ*NYSV*YHNGEKCHYTF
AQIHRIYNTKS*T*CKLWTLGDNDVPM*VHKL*
QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLFGSR
DQFYGRQFFHR*LGEWGYDLGMKL*NYSTSDQQ
ALVRFS*GTCNLDLLHAQLKIGFALL*ESNATT
DLTEGGAQLVMLAHPLRGPVSNRPPTGTGPWPGG
WGPLGYLCTFCSVFL*TYNRFKIRSIFKKEKRR
*PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q*G
FKYISYTFQ*FVRQEPSILELCAFVCVCVCVCVC
L*YKGYTEWHNDQSHAEIYKCCPTQTPSSSSTV
ESKFNDSSILLKGCIQKIFT*C*NSRIKTNSKK
*E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAILKQ
LFELFELFQITHIKCDFINT*YHTI*NLQN*WV
TEARACDLSTLEANRGGLLEARSSTAAWATKQD
LVSTTQK*INAWRHSCSPSYSRG*GKRSA*AQEF
NTSVG*DCATVLTPA*AAEQDSISKSK*INKTY
KILKIK*NMSGLYLPHLN*GLEELNILLQMKHF
MHRLAHSK*SIGVNNLCYCYFLESN*QIPQ*MTS
QGCNQQDPEYGNFY*INNSISSATIQERERREA
IHFKRLKKYMNQICMRQSEKLTPTVLRNYLILV
W**DCCYVF*KVIIL*IFIIK*L*MK

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS
RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG
VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL
CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT
SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL
SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP
LLPMVCVHHSASLYLSYPSIPGFIIFFPRGPQI
FLWQLLKFYHLPSLKSHIPM*YKGLSIMHSANP
SRRGLINLLLY*TCPQREGHRNKAEAMCRCSQAE
GK*DLFDYQVVKCW*EGSSRM*CRTSGQSYSHF
TPVLFPHHRGS*GHSVFQVSRDTGSAQHPHLSC
GQHLSSCGQHHMAEQWAVSHRRCF*DQLPLQE*S
FLLQDQLPHLPPFC**DL*LQGGALGPGPASSE
TLGA*DSSPYVRAHRDCGLCPGVVCGPHGHCGG
HCLHHPRPAFSWCFQTPRAIVSASPSTGAEEWTC
*MT*HYSLARFIISLFSSKYFSSHLFSGT*AAI
SPQSSQMPLHSFPDLLIFFFFSQMLPTKTCLG*
ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFPSFI
QG*LKLWLIIGVLLCFNPI*PHFPDHFSCPVTQ
KPPSTV*PDNMLIS*LTLIFLASLCSHPWHCHP
PLNSGNNEINGYRLPLAQNCYSKNFRTKK*VCTN
FNVAFKSMTEK*VRIKEI*ISKISKVKMYSQNF
KFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQKN
IRIRQ*CGCLKRNKYLFLT*G**QWEKGRE*KPQ
TYLGALNRGAVCLTS*MKLY*ITM*LSLCHSGM
KETV**IWLQDVSWKHANIFRNPQPFPLTPPD*
GKHELMRETLGRTTQLRQCSSSSGCCVLHWIRHL
RGETLTEEMGQQRPHGSKQL*NP*GYF*PCYLH
Q*V*ILIGEKIQNINAIIDTV*FGAKNPKSKSR
TQYFEASILNFINV*SL*HFWPYFSHPQCRSNNT
FLAELLIKFE*F*YLVNALHIVHEHIRKHCGYI
YN*FI*KNGSYYMKSTFVFLSLLMI*EIQM*SY
G*ISFHMIIEGYFSFSRMREAEIGLVRELLALEV
VIFDFGFQLSKREYIPSLWPISVYSRYIIYNRS
LCLFTLGKK*R*FKEKII*NTDWESVSTGNLNQ
**S*KCPSQSTDDFQGNKTILFDTTMVKNVIIHL
LKSTEYITPRVRLNVNYGLWVIMMCQCKFINYS
KCTTQMGDVANGGGYACVGAECIWQQSPAFLAA
GTSFMEDNFSTDSWGNGGMIWG*NCETIPPQINK
H*LDSHKEHAT*ISCMHS*K*GSHSYENLMPPL
I*RKAGLSW*CWLTHCVVQFLTGHQLVLVHGPG
VGDLWDTSAPSAQFFCEPTTALK*GLFLKRKREG
NQL*S*MCKIKILV*KTVTLKCTTC*EAFSNRV
SNIFHIHFNDS*GKNPAFWSCVHLCVCVCVCVC
VCNIRGILNGIMTRVMQKSTNAAQLRLLPQAALW
KANLMIAVFY*KDVFKKYLRNVKIAELKLILKN
KSK*FFLSAKNVGPTINRVTDLPA*FKIMQFLN
NYLSYLNYFRLHT*SVTSLILNITLFKIYKISGS
QRLVLVILALWRPIGEDCLKPGVQQLPGQQSKT
LSLQHKNKLMHGGTPVVPATLEAEARGVLEPRS
SILQWAKIVPLCSLQPRQQSRTPSLKVNK*IKLT
KF*KSNEICQVCTYHI*TRDLKN*TFYYK*STS
CTDWHIVSSR*VLTICVIVIFWSPTNKSHSE*H
HRDATNKIQNMETSTR*TTPFLQQQFKREREEKL
YILKG*RNI*TKFV*GNQKN*HRLY*GII*F*C
GNEIAVMFSKKSLYFRFS**NNYE*

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP
GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV
SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS
SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP
VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*
VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY
RCYQWYASTILPLFT*VIPPYQGSLFSSQEVPRS
SYGNC*NFIISHL*NHIFPCNTRVFPLCIQQIL
LGEVSSTSYFIKHAHREKGTGIKQRQCVVAPKQ
KVNKTSLTIRW*NAGRRALPGCNAELQGRAIHTS
HQCCFLTTEVPEVTVFSKSPVTLGQPNTLICLV
DNIFPPVVNITWLSNGQSVTEGVSETSFLSKSD
HSFFKISYLTFLPSADEIYDCKVEHWGLDQPLLK
HWEPEIPAPMSELTETVVCALGLSVGLMGIVVG
TVFIIQGLRSVGASRHQGPL*VHRHLQEQKNGL
AK*PSTILWPDLSYPFSPPNISPLTFSLGLKLLY
PLRAHKCLYILSLTS*FFFSFLKCYLQRHAWGK
PPGYLIPQ*PPSKISKEAINSFYEIYVKFFHLS
SRAD*NYG**LGYSYVSIQFNLISQIIFHVQ*HR
SHQVQYSLIIC*FLS*H*YFLLPCVPTLGTATH
PSIQATMKLMDTVCPWPRIVIAKILEPKNKSVL
ISMWLLKV*QRNKLG*RKFESQKYQK*KCILKTL
NL*RMMTVEAFLFPCHLEIKIL*AGKEMEVRKT
LE*DNNVGV*KGTNTYSSHRVSDNGKRDGSRSH
RHI*EP*IEAQSASPPE*SCTR*PCSFPCATLA*
RRQYSEYGYRMFLGNMPISSEIPSPFP*PLLTK
ESMSL*EKP*EEQHS*DNVAAAVGAVSSTGFAI
SEEKLSQRKWVSSDLMALNSYEIHEDISNHATCI
SEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPNPG
LSTLKLVF*TL*MCEVSNISGLTFLIHNVGVII
LSLQSY**NLNNFDI*SMPYT*YMNT*ENIVVIF
IINLFKRMDHII*KALLFFSAS**FRRFKCRAM
GEFLFI**LKDIFLFPE*ERLRLDW*ENS*H*K
L*YLTLVFNSLRGNIFPPYGP*VFIQGISYTTDL
YACLLWGRSEDSSRRK*FKTQTGNQ*AQEI*TS
DDHENVHHRAQMIFRAIKLFCLIPQW*KMSLYI
CSNPQNI*HQELDLM*TMDFG***CANVSS*IIA
NVPLRWEMLLMGEAMHVWEQSVSGSSPQPFWQQ
GPVLWKTIFPQIVGGMGV*SGDETVKLFHLRST
SIS*ILIRNMQPRSLACTVKNRVRTPMRI*CHH*
SDGRRGSVGNAGSPTAWSSF*QATNWYWSMARG
LGTSGIPLHLLLSFSVNLQPL*NKVYF*KGKEK
VTNYDPKCVK*KF*YEKQSH*NAQRAKKLLAIGF
QIYFIYISMIREARTQHFGVVCICVCVCVCVCV
SVI*GVY*MA**PESCRNLQMLPNSDSFLKQHC
GKQI***QYFIKRMYSKNIYVMLK*QN*N*F*KI
RVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS*T
II*AI*IISDYTHKV*LH*YLISHYLKFTKLVG
HRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNKARP
CLYNTKIN*CMAALL*SQLL*RLRQEECLSPGV
QYFSGLRLCHCAHSSLGSRAGLHL*K*INK*NL
QNFKNQMKYVRFVPTTFKLGT*RIKHFITNEALH
AQTGT**VVDRC*QFVLLLFSGVQLTNPTVNDI
TGMQPTRSRIWKLLLDKQLHFFSNNSREREKRS
YTF*KAEEIYEPNLYEAIRKTDTDCIKELSNFSV
VMRLLLCFLKSHYTLDFHNKIIMNE

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