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Sequences

Sequence HLA-DQA1.gAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC
AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG
TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT
CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC
AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA
GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC
GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC
CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA
GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGTGTTA
CCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATC
TATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGA
TCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGT
TCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTA
GAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAA
AGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGG
AAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGG
AAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTA
GCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCC
TTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCT
GTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGG
ATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCA
AAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTC
ATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACA
CATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATT
TAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGG
TAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTC
AAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCA
GTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAA
TGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTG
CCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAG
CAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTG
AAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTC
CTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAA
CTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTC
TATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTG
CTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCA
GACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGC
AGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGA
TTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTAT
TTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACA
GCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCA
AGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAA
AAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAAC
ATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACT
AACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAAT
TCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTA
TTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGA
AA
  • HLA-DQA1.F3 (forward primer)
  • HLA-DQA1.R3 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF
QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC
LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA
LLLGALALTTVMSPCGGEDIVADHVASCGVNLY
QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE
FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST
AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF
PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK
ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWVL
PTKTCLG*ATRLPNSSVTSI*NLQGSNKFLL*D
LCQIFPSFIQG*LKLWLIIGVLLCFNPI*PHFPD
HFSCPVTQKPPSTV*PDNMLIS*LTLIFLASLC
SHPWHCHPPLNSGNNEINGYRLPLAQNCYSKNF
RTKK*VCTNFNVAFKSMTEK*VRIKEI*ISKISK
VKMYSQNFKFMKNDDSRSLPLPLSP*DKNSLGR
KRNGSQKNIRIRQ*CGCLKRNKYLFLT*G**QW
EKGRE*KPQTYLGALNRGAVCLTS*MKLY*ITM*
LSLCHSGMKETV**IWLQDVSWKHANIFRNPQP
FPLTPPD*GKHELMRETLGRTTQLRQCSSSSGC
CVLHWIRHLRGETLTEEMGQQRPHGSKQL*NP*G
YF*PCYLHQ*V*ILIGEKIQNINAIIDTV*FGA
KNPKSKSRTQYFEASILNFINV*SL*HFWPYFS
HPQCRSNNTFLAELLIKFE*F*YLVNALHIVHEH
IRKHCGYIYN*FI*KNGSYYMKSTFVFLSLLMI
*EIQM*SYG*ISFHMIIEGYFSFSRMREAEIGL
VRELLALEVVIFDFGFQLSKREYIPSLWPISVYS
RYIIYNRSLCLFTLGKK*R*FKEKII*NTDWES
VSTGNLNQ**S*KCPSQSTDDFQGNKTILFDTT
MVKNVIIHLLKSTEYITPRVRLNVNYGLWVIMMC
QCKFINYSKCTTQMGDVANGGGYACVGAECIWQ
QSPAFLAAGTSFMEDNFSTDSWGNGGMIWG*NC
ETIPPQINKH*LDSHKEHAT*ISCMHS*K*GSHS
YENLMPPLI*RKAGLSW*CWLTHCVVQFLTGHQ
LVLVHGPGVGDLWDTSAPSAQFFCEPTTALK*G
LFLKRKREGNQL*S*MCKIKILV*KTVTLKCTTC
*EAFSNRVSNIFHIHFNDS*GKNPAFWSCVHLC
VCVCVCVCVCNIRGILNGIMTRVMQKSTNAAQL
RLLPQAALWKANLMIAVFY*KDVFKKYLRNVKIA
ELKLILKNKSK*FFLSAKNVGPTINRVTDLPA*
FKIMQFLNNYLSYLNYFRLHT*SVTSLILNITL
FKIYKISGSQRLVLVILALWRPIGEDCLKPGVQQ
LPGQQSKTLSLQHKNKLMHGGTPVVPATLEAEA
RGVLEPRSSILQWAKIVPLCSLQPRQQSRTPSL
KVNK*IKLTKF*KSNEICQVCTYHI*TRDLKN*T
FYYK*STSCTDWHIVSSR*VLTICVIVIFWSPT
NKSHSE*HHRDATNKIQNMETSTR*TTPFLQQQ
FKREREEKLYILKG*RNI*TKFV*GNQKN*HRLY
*GII*F*CGNEIAVMFSKKSLYFRFS**NNYE*

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS
RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG
VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL
CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT
SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL
SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP
LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF
LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS
RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGCY
LQRHAWGKPPGYLIPQ*PPSKISKEAINSFYEI
YVKFFHLSSRAD*NYG**LGYSYVSIQFNLISQ
IIFHVQ*HRSHQVQYSLIIC*FLS*H*YFLLPCV
PTLGTATHPSIQATMKLMDTVCPWPRIVIAKIL
EPKNKSVLISMWLLKV*QRNKLG*RKFESQKYQ
K*KCILKTLNL*RMMTVEAFLFPCHLEIKIL*AG
KEMEVRKTLE*DNNVGV*KGTNTYSSHRVSDNG
KRDGSRSHRHI*EP*IEAQSASPPE*SCTR*PC
SFPCATLA*RRQYSEYGYRMFLGNMPISSEIPSP
FP*PLLTKESMSL*EKP*EEQHS*DNVAAAVGA
VSSTGFAISEEKLSQRKWVSSDLMALNSYEIHE
DISNHATCISEFKF*LEKKYKTLMQ*LIQYSLVQ
RTLNPNPGLSTLKLVF*TL*MCEVSNISGLTFL
IHNVGVIILSLQSY**NLNNFDI*SMPYT*YMN
T*ENIVVIFIINLFKRMDHII*KALLFFSAS**F
RRFKCRAMGEFLFI**LKDIFLFPE*ERLRLDW
*ENS*H*KL*YLTLVFNSLRGNIFPPYGP*VFI
QGISYTTDLYACLLWGRSEDSSRRK*FKTQTGNQ
*AQEI*TSDDHENVHHRAQMIFRAIKLFCLIPQ
W*KMSLYICSNPQNI*HQELDLM*TMDFG***C
ANVSS*IIANVPLRWEMLLMGEAMHVWEQSVSGS
SPQPFWQQGPVLWKTIFPQIVGGMGV*SGDETV
KLFHLRSTSIS*ILIRNMQPRSLACTVKNRVRT
PMRI*CHH*SDGRRGSVGNAGSPTAWSSF*QATN
WYWSMARGLGTSGIPLHLLLSFSVNLQPL*NKV
YF*KGKEKVTNYDPKCVK*KF*YEKQSH*NAQR
AKKLLAIGFQIYFIYISMIREARTQHFGVVCICV
CVCVCVCVSVI*GVY*MA**PESCRNLQMLPNS
DSFLKQHCGKQI***QYFIKRMYSKNIYVMLK*
QN*N*F*KIRVNSSFYQLKM*GLLSIGLQIYLPD
LKSCNS*TII*AI*IISDYTHKV*LH*YLISHY
LKFTKLVGHRGSCL*S*HFGGQ*GRIA*SQEFN
SCLGNKARPCLYNTKIN*CMAALL*SQLL*RLRQ
EECLSPGVQYFSGLRLCHCAHSSLGSRAGLHL*
K*INK*NLQNFKNQMKYVRFVPTTFKLGT*RIK
HFITNEALHAQTGT**VVDRC*QFVLLLFSGVQL
TNPTVNDITGMQPTRSRIWKLLLDKQLHFFSNN
SREREKRSYTF*KAEEIYEPNLYEAIRKTDTDC
IKELSNFSVVMRLLLCFLKSHYTLDFHNKIIMNE

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP
GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV
SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS
SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP
VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*
VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY
RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS
SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ
DQLPHLPPFC**DL*LQGGALGPGPASSETLGV
TYKDMPGVSHPAT*FLSNLHLKSPRKQ*IPFMRS
MSNFSIFHPGLTETMANNWGTLMFQSNLTSFPR
SFFMSSNTEATKYSIA**YVDFLADINISCFLV
FPPLALPPTPQFRQQ*N*WIPSALGPELL*QKF*
NQKISLY*FQCGF*KYDREIS*DKGNLNLKNIK
SKNVFSKL*IYEE**Q*KPSSSLVTLR*KFFRQ
EKKWKSEKH*NKTIMWVSEKEQILIPHIGLVTMG
KGTGVEATDISRSPE*RRSLPHLLNEAVLDNHV
AFPVPLWHEGDSIVNMATGCFLETCQYLQKSPA
LSPNPS*LRKA*AYERNPRKNNTVETM*QQQWVL
CPPLDSPSPRRNSHRGNGSAATSWL*TAMKSMR
IFLTMLPASVSLNFNWRKNTKH*CNN*YSIVWC
KEP*IQIQDSVL*S*YFKLYKCVKSLTFLALLFS
STM*E**YFPCRVIDKI*IILIFSQCLTHST*T
HKKTLWLYL*LIYLKEWIILYEKHFCFSQPLND
LGDSNVELWVNFFSYDN*RIFFFFQNERG*DWIG
KRTLSTRSCNI*LWFSTL*EGIYSLLMAHKCLF
KVYHIQQIFMPVYFGEEVKIVQGENNLKHRLGI
SKHRKSEPVMIMKMSITEHR*FSGQ*NYSV*YHN
GEKCHYTFAQIHRIYNTKS*T*CKLWTLGDNDV
PM*VHKL*QMYHSDGRCC*WGRLCMCGSRVYLA
AVPSLFGSRDQFYGRQFFHR*LGEWGYDLGMKL*
NYSTSDQQALVRFS*GTCNLDLLHAQLKIGFAL
L*ESNATTDLTEGGAQLVMLAHPLRGPVSNRPP
TGTGPWPGGWGPLGYLCTFCSVFL*TYNRFKIRS
IFKKEKRR*PIMILNV*NKNFSMKNSHIKMHNV
LRSF*Q*GFKYISYTFQ*FVRQEPSILELCAFV
CVCVCVCVCL*YKGYTEWHNDQSHAEIYKCCPTQ
TPSSSSTVESKFNDSSILLKGCIQKIFT*C*NS
RIKTNSKK*E*IVLFIS*KCRAYYQ*GYRSTCL
I*NHAILKQLFELFELFQITHIKCDFINT*YHTI
*NLQN*WVTEARACDLSTLEANRGGLLEARSST
AAWATKQDLVSTTQK*INAWRHSCSPSYSRG*G
KRSA*AQEFNTSVG*DCATVLTPA*AAEQDSISK
SK*INKTYKILKIK*NMSGLYLPHLN*GLEELN
ILLQMKHFMHRLAHSK*SIGVNNLCYCYFLESN
*QIPQ*MTSQGCNQQDPEYGNFY*INNSISSATI
QERERREAIHFKRLKKYMNQICMRQSEKLTPTV
LRNYLILVW**DCCYVF*KVIIL*IFIIK*L*M
K

Sequence HLA-DQA1.bAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC
AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG
TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT
CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC
AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA
GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC
GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC
CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA
GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCT
GAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCA
TCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAAT
GGACTTGCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGC
TGCTATATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTG
GGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCAT
CTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGT
AACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGC
CACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCT
GTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCA
AAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAG
AAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGA
AGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTC
TGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCT
GACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTC
GCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTA
CCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAA
TCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTA
ATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGG
TTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAG
AGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCAC
TAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAA
CAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTG
AACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTA
TACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAA
TTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTG
GCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGA
TCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCC
ACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGC
CCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAA
GAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAA
TAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAG
CACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCT
AAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTC
TTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTA
GTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAG
CAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCC
AGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAA
AACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAG
CACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAA
TGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAG
AGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATT
TTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA
  • HLA-DQA1.F3 (forward primer)
  • HLA-DQA1.R3 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF
QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC
LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA
LLLGALALTTVMSPCGGEDIVADHVASCGVNLY
QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE
FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST
AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF
PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK
ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWEP
EIPAPMSELTETVVCALGLSVGLMGIVVGTVFI
IQGLRSVGASRHQGPL*IPSWKGRCIAIYRSRRM
DLLNDLALFSGPIYHIPFLLQIFLLSPFLWDLS
CYIPSELTNAFTFFP*PPDFFFLFSNVTYKDMP
GVSHPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSI
FHPGLTETMANNWGTLMFQSNLTSFPRSFFMSS
NTEATKYSIA**YVDFLADINISCFLVFPPLAL
PPTPQFRQQ*N*WIPSALGPELL*QKF*NQKISL
Y*FQCGF*KYDREIS*DKGNLNLKNIKSKNVFS
KL*IYEE**Q*KPSSSLVTLR*KFFRQEKKWKS
EKH*NKTIMWVSEKEQILIPHIGLVTMGKGTGVE
ATDISRSPE*RRSLPHLLNEAVLDNHVAFPVPL
WHEGDSIVNMATGCFLETCQYLQKSPALSPNPS
*LRKA*AYERNPRKNNTVETM*QQQWVLCPPLDS
PSPRRNSHRGNGSAATSWL*TAMKSMRIFLTML
PASVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQ
IQDSVL*S*YFKLYKCVKSLTFLALLFSSTM*E*
*YFPCRVIDKI*IILIFSQCLTHST*THKKTLW
LYL*LIYLKEWIILYEKHFCFSQPLNDLGDSNV
ELWVNFFSYDN*RIFFFFQNERG*DWIGKRTLST
RSCNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQ
QIFMPVYFGEEVKIVQGENNLKHRLGISKHRKS
EPVMIMKMSITEHR*FSGQ*NYSV*YHNGEKCHY
TFAQIHRIYNTKS*T*CKLWTLGDNDVPM*VHK
L*QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLF
GSRDQFYGRQFFHR*LGEWGYDLGMKL*NYSTSD
QQALVRFS*GTCNLDLLHAQLKIGFALL*ESNA
TTDLTEGGAQLVMLAHPLRGPVSNRPPTGTGPW
PGGWGPLGYLCTFCSVFL*TYNRFKIRSIFKKEK
RR*PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q
*GFKYISYTFQ*FVRQEPSILELCAFVCVCVCV
CVCL*YKGYTEWHNDQSHAEIYKCCPTQTPSSSS
TVESKFNDSSILLKGCIQKIFT*C*NSRIKTNS
KK*E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAI
LKQLFELFELFQITHIKCDFINT*YHTI*NLQN*
WVTEARACDLSTLEANRGGLLEARSSTAAWATK
QDLVSTTQK*INAWRHSCSPSYSRG*GKRSA*A
QEFNTSVG*DCATVLTPA*AAEQDSISKSK*INK
TYKILKIK*NMSGLYLPHLN*GLEELNILLQMK
HFMHRLAHSK*SIGVNNLCYCYFLESN*QIPQ*
MTSQGCNQQDPEYGNFY*INNSISSATIQERERR
EAIHFKRLKKYMNQICMRQSEKLTPTVLRNYLI
LVW**DCCYVF*KVIIL*IFIIK*L*MK

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS
RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG
VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL
CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT
SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL
SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP
LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF
LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS
RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGSL
RFQPLCQSSQRLWSVPWGCLWASWALWWALSSS
SKACVQLVLPDTKGHCESHPGREGASPSTGAEE
WTC*MT*HYSLARFIISLFSSKYFSSHLFSGT*A
AISPQSSQMPLHSFPDLLIFFFFSQMLPTKTCL
G*ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFP
SFIQG*LKLWLIIGVLLCFNPI*PHFPDHFSCPV
TQKPPSTV*PDNMLIS*LTLIFLASLCSHPWHC
HPPLNSGNNEINGYRLPLAQNCYSKNFRTKK*V
CTNFNVAFKSMTEK*VRIKEI*ISKISKVKMYSQ
NFKFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQ
KNIRIRQ*CGCLKRNKYLFLT*G**QWEKGRE*
KPQTYLGALNRGAVCLTS*MKLY*ITM*LSLCHS
GMKETV**IWLQDVSWKHANIFRNPQPFPLTPP
D*GKHELMRETLGRTTQLRQCSSSSGCCVLHWI
RHLRGETLTEEMGQQRPHGSKQL*NP*GYF*PCY
LHQ*V*ILIGEKIQNINAIIDTV*FGAKNPKSK
SRTQYFEASILNFINV*SL*HFWPYFSHPQCRS
NNTFLAELLIKFE*F*YLVNALHIVHEHIRKHCG
YIYN*FI*KNGSYYMKSTFVFLSLLMI*EIQM*
SYG*ISFHMIIEGYFSFSRMREAEIGLVRELLA
LEVVIFDFGFQLSKREYIPSLWPISVYSRYIIYN
RSLCLFTLGKK*R*FKEKII*NTDWESVSTGNL
NQ**S*KCPSQSTDDFQGNKTILFDTTMVKNVI
IHLLKSTEYITPRVRLNVNYGLWVIMMCQCKFIN
YSKCTTQMGDVANGGGYACVGAECIWQQSPAFL
AAGTSFMEDNFSTDSWGNGGMIWG*NCETIPPQ
INKH*LDSHKEHAT*ISCMHS*K*GSHSYENLMP
PLI*RKAGLSW*CWLTHCVVQFLTGHQLVLVHG
PGVGDLWDTSAPSAQFFCEPTTALK*GLFLKRK
REGNQL*S*MCKIKILV*KTVTLKCTTC*EAFSN
RVSNIFHIHFNDS*GKNPAFWSCVHLCVCVCVC
VCVCNIRGILNGIMTRVMQKSTNAAQLRLLPQA
ALWKANLMIAVFY*KDVFKKYLRNVKIAELKLIL
KNKSK*FFLSAKNVGPTINRVTDLPA*FKIMQF
LNNYLSYLNYFRLHT*SVTSLILNITLFKIYKI
SGSQRLVLVILALWRPIGEDCLKPGVQQLPGQQS
KTLSLQHKNKLMHGGTPVVPATLEAEARGVLEP
RSSILQWAKIVPLCSLQPRQQSRTPSLKVNK*I
KLTKF*KSNEICQVCTYHI*TRDLKN*TFYYK*S
TSCTDWHIVSSR*VLTICVIVIFWSPTNKSHSE
*HHRDATNKIQNMETSTR*TTPFLQQQFKRERE
EKLYILKG*RNI*TKFV*GNQKN*HRLY*GII*F
*CGNEIAVMFSKKSLYFRFS**NNYE*

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP
GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV
SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS
SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP
VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*
VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY
RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS
SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ
DQLPHLPPFC**DL*LQGGALGPGPASSETLGA
*DSSPYVRAHRDCGLCPGVVCGPHGHCGGHCLHH
PRPAFSWCFQTPRAIVNPILEGKVHRHLQEQKN
GLAK*PSTILWPDLSYPFSPPNISPLTFSLGLK
LLYPLRAHKCLYILSLTS*FFFSFLKCYLQRHAW
GKPPGYLIPQ*PPSKISKEAINSFYEIYVKFFH
LSSRAD*NYG**LGYSYVSIQFNLISQIIFHVQ
*HRSHQVQYSLIIC*FLS*H*YFLLPCVPTLGTA
THPSIQATMKLMDTVCPWPRIVIAKILEPKNKS
VLISMWLLKV*QRNKLG*RKFESQKYQK*KCIL
KTLNL*RMMTVEAFLFPCHLEIKIL*AGKEMEVR
KTLE*DNNVGV*KGTNTYSSHRVSDNGKRDGSR
SHRHI*EP*IEAQSASPPE*SCTR*PCSFPCAT
LA*RRQYSEYGYRMFLGNMPISSEIPSPFP*PLL
TKESMSL*EKP*EEQHS*DNVAAAVGAVSSTGF
AISEEKLSQRKWVSSDLMALNSYEIHEDISNHA
TCISEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPN
PGLSTLKLVF*TL*MCEVSNISGLTFLIHNVGV
IILSLQSY**NLNNFDI*SMPYT*YMNT*ENIV
VIFIINLFKRMDHII*KALLFFSAS**FRRFKCR
AMGEFLFI**LKDIFLFPE*ERLRLDW*ENS*H
*KL*YLTLVFNSLRGNIFPPYGP*VFIQGISYT
TDLYACLLWGRSEDSSRRK*FKTQTGNQ*AQEI*
TSDDHENVHHRAQMIFRAIKLFCLIPQW*KMSL
YICSNPQNI*HQELDLM*TMDFG***CANVSS*
IIANVPLRWEMLLMGEAMHVWEQSVSGSSPQPFW
QQGPVLWKTIFPQIVGGMGV*SGDETVKLFHLR
STSIS*ILIRNMQPRSLACTVKNRVRTPMRI*C
HH*SDGRRGSVGNAGSPTAWSSF*QATNWYWSMA
RGLGTSGIPLHLLLSFSVNLQPL*NKVYF*KGK
EKVTNYDPKCVK*KF*YEKQSH*NAQRAKKLLA
IGFQIYFIYISMIREARTQHFGVVCICVCVCVCV
CVSVI*GVY*MA**PESCRNLQMLPNSDSFLKQ
HCGKQI***QYFIKRMYSKNIYVMLK*QN*N*F
*KIRVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS
*TII*AI*IISDYTHKV*LH*YLISHYLKFTKL
VGHRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNK
ARPCLYNTKIN*CMAALL*SQLL*RLRQEECLSP
GVQYFSGLRLCHCAHSSLGSRAGLHL*K*INK*
NLQNFKNQMKYVRFVPTTFKLGT*RIKHFITNE
ALHAQTGT**VVDRC*QFVLLLFSGVQLTNPTVN
DITGMQPTRSRIWKLLLDKQLHFFSNNSREREK
RSYTF*KAEEIYEPNLYEAIRKTDTDCIKELSN
FSVVMRLLLCFLKSHYTLDFHNKIIMNE

Sequence HLA-DQA1.eAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC
AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG
TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT
CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGGATCCATCTCTGACTCCCTGCTCCTTTATAGAGATG
GACAGTGGGTTTGTAAAACAAAAGTTGAAAAGTCAGATAGTTAAAAGGGGAAGTGAACTGGAAGGTACTTTAAACTTTCACAACTTTATTAACCGTGGCT
GCTCCCATTATGATTTTGTTCGGCAGTGGAAGTTTCACCTGCTTCTCCAGAGCGCTTGGCGTCTTTGTTCCAAATTTCCTTTCTTCAACCTCACACCAGA
GTGCCCTGGTCAGGCTTGGCTCATTCATTAGGCACAATGTGGGCAGTGCAGGGGACCCTCCATACTGTAAAGCCACATGAGAAGGTTTTAACTCCTTTTA
AAATTAGAAAAATAATGGAATATTAGAGCCTAAGAAAATGTTTTAACTTTTAATTCAGCCTAGATTATATTGTCTTTATACCAATTCAGTCATAAAATAT
AATTTTCCATATTTTTGTGGAGGAAGAGGCCCATACAAGCAAGAATGCTCGGGGCTCACATGTCAGAATGCAGCCTTGGTCATGACTGATCCTGGCCTTC
GTATGGTTCTGCTACCTGTGTGCCTGCCCGTCTTCCCCAAAATCTATGTGGTCCTCGAATATAGCAACTGTCATTCAATACACATGTTTGAGCACACAGT
GAGCTAAGTTTTAAGGATTCAAAGATGAAAAGTCATGCTGTCTTCCCTGCAGAGGGTGCTCAGATTAGTGATGGAAACAGTATGGGGTGCAGGAAAGCAG
AAGGCCATTGCTGAGCAGGGCAGTGGACCCAGCAGAGGCTGAAACAATACAAGTGACTTGGTTCCAGGTGGGCCAGCAGGGTGACGTCCTCTAGCAAAAT
TTGGCACCCAAGACAAGTACCAGAAGAAAAGAAAGACTGCATGTATTCCACATATATTCATGTTTGAACAAGGAGTCAAGATTTATGGCAAGGATAAGGA
GGCTTTGTTGGTGACCTGTTAAGACCAACCAGGGCAGTCATGCTGGATAGGGAAGAAGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTT
ACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAG
CAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCT
ACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTG
TGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAG
TTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATT
CCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAG
GCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAATGGACTT
GCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGCTGCTAT
ATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTGGGGTAA
GCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCA
TCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACA
GAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCA
CCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTA
ATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTT
TAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAAC
ATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCAC
AGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCAT
GAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAA
GGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATC
TCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCA
TCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGG
ACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATA
CTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATAT
TTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTAT
GGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAG
TTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATC
TTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAG
TGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATT
TGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAG
CAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCA
GGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACA
AGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACT
GATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGG
GTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAG
GTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGT
TTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGT
GGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAA
TAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAAC
AATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGT
CACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGAC
CTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGT
TCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTA
CAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTC
ATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACAT
CACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGC
TATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTG
TGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA
  • HLA-DQA1.F3 (forward primer)
  • HLA-DQA1.R3 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF
QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC
LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA
LLLGALALTTVMSPCGGEDIVGSISDSLLLYRD
GQWVCKTKVEKSDS*KGK*TGRYFKLSQLY*PWL
LPL*FCSAVEVSPASPERLASLFQISFLQPHTR
VPWSGLAHSLGTMWAVQGTLHTVKPHEKVLTPF
KIRKIMEY*SLRKCFNF*FSLDYIVFIPIQS*NI
IFHIFVEEEAHTSKNARGSHVRMQPWS*LILAF
VWFCYLCACPSSPKSMWSSNIATVIQYTCLSTQ
*AKF*GFKDEKSCCLPCRGCSD**WKQYGVQESR
RPLLSRAVDPAEAETIQVTWFQVGQQGDVL*QN
LAPKTSTRRKERLHVFHIYSCLNKESRFMARIR
RLCW*PVKTNQGSHAG*GRS*PRCLLWCKLVPVL
RSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*VQ
QIWRF*PAGCTEKHGCGKTQLEHHD*TLQLYRC
YQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLSSC
GQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQDQ
LPHLPPFC**DL*LQGGALGPGPASSETLGA*D
SSPYVRAHRDCGLCPGVVCGPHGHCGGHCLHHPR
PAFSWCFQTPRAIVNPILEGKVHRHLQEQKNGL
AK*PSTILWPDLSYPFSPPNISPLTFSLGLKLL
YPLRAHKCLYILSLTS*FFFSFLKCYLQRHAWGK
PPGYLIPQ*PPSKISKEAINSFYEIYVKFFHLS
SRAD*NYG**LGYSYVSIQFNLISQIIFHVQ*H
RSHQVQYSLIIC*FLS*H*YFLLPCVPTLGTATH
PSIQATMKLMDTVCPWPRIVIAKILEPKNKSVL
ISMWLLKV*QRNKLG*RKFESQKYQK*KCILKT
LNL*RMMTVEAFLFPCHLEIKIL*AGKEMEVRKT
LE*DNNVGV*KGTNTYSSHRVSDNGKRDGSRSH
RHI*EP*IEAQSASPPE*SCTR*PCSFPCATLA
*RRQYSEYGYRMFLGNMPISSEIPSPFP*PLLTK
ESMSL*EKP*EEQHS*DNVAAAVGAVSSTGFAI
SEEKLSQRKWVSSDLMALNSYEIHEDISNHATC
ISEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPNPG
LSTLKLVF*TL*MCEVSNISGLTFLIHNVGVII
LSLQSY**NLNNFDI*SMPYT*YMNT*ENIVVI
FIINLFKRMDHII*KALLFFSAS**FRRFKCRAM
GEFLFI**LKDIFLFPE*ERLRLDW*ENS*H*K
L*YLTLVFNSLRGNIFPPYGP*VFIQGISYTTD
LYACLLWGRSEDSSRRK*FKTQTGNQ*AQEI*TS
DDHENVHHRAQMIFRAIKLFCLIPQW*KMSLYI
CSNPQNI*HQELDLM*TMDFG***CANVSS*II
ANVPLRWEMLLMGEAMHVWEQSVSGSSPQPFWQQ
GPVLWKTIFPQIVGGMGV*SGDETVKLFHLRST
SIS*ILIRNMQPRSLACTVKNRVRTPMRI*CHH
*SDGRRGSVGNAGSPTAWSSF*QATNWYWSMARG
LGTSGIPLHLLLSFSVNLQPL*NKVYF*KGKEK
VTNYDPKCVK*KF*YEKQSH*NAQRAKKLLAIG
FQIYFIYISMIREARTQHFGVVCICVCVCVCVCV
SVI*GVY*MA**PESCRNLQMLPNSDSFLKQHC
GKQI***QYFIKRMYSKNIYVMLK*QN*N*F*K
IRVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS*T
II*AI*IISDYTHKV*LH*YLISHYLKFTKLVG
HRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNKAR
PCLYNTKIN*CMAALL*SQLL*RLRQEECLSPGV
QYFSGLRLCHCAHSSLGSRAGLHL*K*INK*NL
QNFKNQMKYVRFVPTTFKLGT*RIKHFITNEAL
HAQTGT**VVDRC*QFVLLLFSGVQLTNPTVNDI
TGMQPTRSRIWKLLLDKQLHFFSNNSREREKRS
YTF*KAEEIYEPNLYEAIRKTDTDCIKELSNFS
VVMRLLLCFLKSHYTLDFHNKIIMNE

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS
RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG
VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL
CCWGPSL*PP**APVEVKTLWDPSLTPCSFIEM
DSGFVKQKLKSQIVKRGSELEGTLNFHNFINRG
CSHYDFVRQWKFHLLLQSAWRLCSKFPFFNLTPE
CPGQAWLIH*AQCGQCRGPSIL*SHMRRF*LLL
KLEK*WNIRA*ENVLTFNSA*IILSLYQFSHKI
*FSIFLWRKRPIQARMLGAHMSECSLGHD*SWPS
YGSATCVPARLPQNLCGPRI*QLSFNTHV*AHS
ELSFKDSKMKSHAVFPAEGAQISDGNSMGCRKA
EGHC*AGQWTQQRLKQYK*LGSRWASRVTSSSKI
WHPRQVPEEKKDCMYSTYIHV*TRSQDLWQG*G
GFVGDLLRPTRAVMLDREEADHVASCGVNLYQF
YGPSGQYTHEFDGDEQFYVDLERKETAWRWPEFS
KFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAA
TNEVPEVTVFSKSPVTLGQPNTLICLVDNIFPP
VVNITWLSNGQSVTEGVSETSFLSKSDHSFFKIS
YLTFLPSADEIYDCKVEHWGLDQPLLKHWEPEI
PAPMSELTETVVCALGLSVGLMGIVVGTVFIIQ
GLRSVGASRHQGPL*IPSWKGRCIAIYRSRRMDL
LNDLALFSGPIYHIPFLLQIFLLSPFLWDLSCY
IPSELTNAFTFFP*PPDFFFLFSNVTYKDMPGV
SHPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSIFH
PGLTETMANNWGTLMFQSNLTSFPRSFFMSSNT
EATKYSIA**YVDFLADINISCFLVFPPLALPP
TPQFRQQ*N*WIPSALGPELL*QKF*NQKISLY*
FQCGF*KYDREIS*DKGNLNLKNIKSKNVFSKL
*IYEE**Q*KPSSSLVTLR*KFFRQEKKWKSEK
H*NKTIMWVSEKEQILIPHIGLVTMGKGTGVEAT
DISRSPE*RRSLPHLLNEAVLDNHVAFPVPLWH
EGDSIVNMATGCFLETCQYLQKSPALSPNPS*L
RKA*AYERNPRKNNTVETM*QQQWVLCPPLDSPS
PRRNSHRGNGSAATSWL*TAMKSMRIFLTMLPA
SVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQIQ
DSVL*S*YFKLYKCVKSLTFLALLFSSTM*E**Y
FPCRVIDKI*IILIFSQCLTHST*THKKTLWLY
L*LIYLKEWIILYEKHFCFSQPLNDLGDSNVEL
WVNFFSYDN*RIFFFFQNERG*DWIGKRTLSTRS
CNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQQI
FMPVYFGEEVKIVQGENNLKHRLGISKHRKSEP
VMIMKMSITEHR*FSGQ*NYSV*YHNGEKCHYTF
AQIHRIYNTKS*T*CKLWTLGDNDVPM*VHKL*
QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLFGS
RDQFYGRQFFHR*LGEWGYDLGMKL*NYSTSDQQ
ALVRFS*GTCNLDLLHAQLKIGFALL*ESNATT
DLTEGGAQLVMLAHPLRGPVSNRPPTGTGPWPG
GWGPLGYLCTFCSVFL*TYNRFKIRSIFKKEKRR
*PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q*G
FKYISYTFQ*FVRQEPSILELCAFVCVCVCVCV
CL*YKGYTEWHNDQSHAEIYKCCPTQTPSSSSTV
ESKFNDSSILLKGCIQKIFT*C*NSRIKTNSKK
*E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAILK
QLFELFELFQITHIKCDFINT*YHTI*NLQN*WV
TEARACDLSTLEANRGGLLEARSSTAAWATKQD
LVSTTQK*INAWRHSCSPSYSRG*GKRSA*AQE
FNTSVG*DCATVLTPA*AAEQDSISKSK*INKTY
KILKIK*NMSGLYLPHLN*GLEELNILLQMKHF
MHRLAHSK*SIGVNNLCYCYFLESN*QIPQ*MT
SQGCNQQDPEYGNFY*INNSISSATIQERERREA
IHFKRLKKYMNQICMRQSEKLTPTVLRNYLILV
W**DCCYVF*KVIIL*IFIIK*L*MK

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP
GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV
SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS
SAAGGPRSDHRDEPLWR*RHCGIHL*LPAPL*RW
TVGL*NKS*KVR*LKGEVNWKVL*TFTTLLTVA
APIMILFGSGSFTCFSRALGVFVPNFLSSTSHQ
SALVRLGSFIRHNVGSAGDPPYCKAT*EGFNSF*
N*KNNGILEPKKMF*LLIQPRLYCLYTNSVIKY
NFPYFCGGRGPYKQECSGLTCQNAALVMTDPGL
RMVLLPVCLPVFPKIYVVLEYSNCHSIHMFEHTV
S*VLRIQR*KVMLSSLQRVLRLVMETVWGAGKQ
KAIAEQGSGPSRG*NNTSDLVPGGPAG*RPLAK
FGTQDKYQKKRKTACIPHIFMFEQGVKIYGKDKE
ALLVTC*DQPGQSCWIGKKLTTLPLVV*TCTSF
TVPLASTPMNLMEMSSSTWTWRGRRLPGGGLSS
ANLEVLTRRVH*ETWLWQNTT*TS*LNATTLPLL
PMRFLRSQCFPSLP*HWVSPTPSFVLWTTSFLL
WSTSHG*AMGSQSQKVFLRPASSPRVIIPSSRS
VTSPSSLLLMRFMTARWSTGAWTSLF*NTGSLRF
QPLCQSSQRLWSVPWGCLWASWALWWALSSSSK
ACVQLVLPDTKGHCESHPGREGASPSTGAEEWT
C*MT*HYSLARFIISLFSSKYFSSHLFSGT*AAI
SPQSSQMPLHSFPDLLIFFFFSQMLPTKTCLG*
ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFPSF
IQG*LKLWLIIGVLLCFNPI*PHFPDHFSCPVTQ
KPPSTV*PDNMLIS*LTLIFLASLCSHPWHCHP
PLNSGNNEINGYRLPLAQNCYSKNFRTKK*VCT
NFNVAFKSMTEK*VRIKEI*ISKISKVKMYSQNF
KFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQKN
IRIRQ*CGCLKRNKYLFLT*G**QWEKGRE*KP
QTYLGALNRGAVCLTS*MKLY*ITM*LSLCHSGM
KETV**IWLQDVSWKHANIFRNPQPFPLTPPD*
GKHELMRETLGRTTQLRQCSSSSGCCVLHWIRH
LRGETLTEEMGQQRPHGSKQL*NP*GYF*PCYLH
Q*V*ILIGEKIQNINAIIDTV*FGAKNPKSKSR
TQYFEASILNFINV*SL*HFWPYFSHPQCRSNN
TFLAELLIKFE*F*YLVNALHIVHEHIRKHCGYI
YN*FI*KNGSYYMKSTFVFLSLLMI*EIQM*SY
G*ISFHMIIEGYFSFSRMREAEIGLVRELLALE
VVIFDFGFQLSKREYIPSLWPISVYSRYIIYNRS
LCLFTLGKK*R*FKEKII*NTDWESVSTGNLNQ
**S*KCPSQSTDDFQGNKTILFDTTMVKNVIIH
LLKSTEYITPRVRLNVNYGLWVIMMCQCKFINYS
KCTTQMGDVANGGGYACVGAECIWQQSPAFLAA
GTSFMEDNFSTDSWGNGGMIWG*NCETIPPQIN
KH*LDSHKEHAT*ISCMHS*K*GSHSYENLMPPL
I*RKAGLSW*CWLTHCVVQFLTGHQLVLVHGPG
VGDLWDTSAPSAQFFCEPTTALK*GLFLKRKRE
GNQL*S*MCKIKILV*KTVTLKCTTC*EAFSNRV
SNIFHIHFNDS*GKNPAFWSCVHLCVCVCVCVC
VCNIRGILNGIMTRVMQKSTNAAQLRLLPQAAL
WKANLMIAVFY*KDVFKKYLRNVKIAELKLILKN
KSK*FFLSAKNVGPTINRVTDLPA*FKIMQFLN
NYLSYLNYFRLHT*SVTSLILNITLFKIYKISG
SQRLVLVILALWRPIGEDCLKPGVQQLPGQQSKT
LSLQHKNKLMHGGTPVVPATLEAEARGVLEPRS
SILQWAKIVPLCSLQPRQQSRTPSLKVNK*IKL
TKF*KSNEICQVCTYHI*TRDLKN*TFYYK*STS
CTDWHIVSSR*VLTICVIVIFWSPTNKSHSE*H
HRDATNKIQNMETSTR*TTPFLQQQFKREREEK
LYILKG*RNI*TKFV*GNQKN*HRLY*GII*F*C
GNEIAVMFSKKSLYFRFS**NNYE*

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