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Sequences

Sequence HLA-DQA1.aAug05

AAGAGCAAAATAATATGAAAAGTATTTAACAAGGAATATAAAGAATCATCAAAGAAAATGTTATAAAACTTCTTTGAGACCACAAGAGATGATAGAGATG GGGTTTCACTGTGTTAGCGAGGATGGTCTTGATCTCCTGACCTCGTGATCCGCCTGCTTCTGCCTCCCAAAGTGCTGGGATTACAGTCGTGAGCCACCGC GCCTGGCCTCCTCATATTATTTTTTATTGTGCAGTTTATTCAAGAGAGCTTTCCAGCACCTGTGATAAGCCTCAGCCCGCCTGCCGGATAATGAGCCCAC ATGGATTGAGAGAGGCCTCCTATCCCTGCCAAACCTATTGATGCTACAGAGATGTGAGGGAGCCCACCTGAGAAAAGCTGAACCTGCCCAGTACATAAAA ACCACTCAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGAT GAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCTGGCCAGTACACCCATGAA TTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTG CACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTT TTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGG CAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATG AGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGAC TGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACAC CAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGTGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATC TAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGG TACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTT GATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATAC CGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAAT AAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCC TCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGG AACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCT GCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGT TTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGA ACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGC AGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACA AAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATA AATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAAT TTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTA TATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATAT TTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGA GGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATA GTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGAT GATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTA GACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGG GGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGAT AGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCT AGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTG GCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCT CAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAA TTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTG AGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATG ACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAA AGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGT AGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACAC ACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGA GGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGG CGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGC TCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTT GTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGT GTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAA ACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCA AATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTC ATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F3 (forward primer)
HLA-DQA1.R3 (reverse primer)
Alternative acceptor

Frame 0

KSKII*KVFNKEYKESSKKML*NFFETTRDDRD GVSLC*RGWS*SPDLVIRLLLPPKVLGLQS*ATA PGLLILFFIVQFIQESFPAPVISLSPPAG**AH MD*ERPPIPAKPIDATEM*GSPPEKS*TCPVHK NHSAQNTNC*GCLGKRMILNKALLLGALALTTVM SPCGGEDIVADHVASCGVNLYQFYGPSGQYTHE FDGDEQFYVDLERKETAWRWPEFSKFGGFDPQG ALRNMAVAKHNLNIMIKRYNSTAATNEVPEVTVF SKSPVTLGQPNTLICLVDNIFPPVVNITWLSNG QSVTEGVSETSFLSKSDHSFFKISYLTFLPSAD EIYDCKVEHWGLDQPLLKHWEPEIPAPMSELTET VVCALGLSVGLMGIVVGTVFIIQGLRSVGASRH QGPL*IPSWKGSVTYKDMPGVSHPAT*FLSNLH LKSPRKQ*IPFMRSMSNFSIFHPGLTETMANNWG TLMFQSNLTSFPRSFFMSSNTEATKYSIA**YV DFLADINISCFLVFPPLALPPTPQFRQQ*N*WI PSALGPELL*QKF*NQKISLY*FQCGF*KYDREI S*DKGNLNLKNIKSKNVFSKL*IYEE**Q*KPS SSLVTLR*KFFRQEKKWKSEKH*NKTIMWVSEK EQILIPHIGLVTMGKGTGVEATDISRSPE*RRSL PHLLNEAVLDNHVAFPVPLWHEGDSIVNMATGC FLETCQYLQKSPALSPNPS*LRKA*AYERNPRK NNTVETM*QQQWVLCPPLDSPSPRRNSHRGNGSA ATSWL*TAMKSMRIFLTMLPASVSLNFNWRKNT KH*CNN*YSIVWCKEP*IQIQDSVL*S*YFKLY KCVKSLTFLALLFSSTM*E**YFPCRVIDKI*II LIFSQCLTHST*THKKTLWLYL*LIYLKEWIIL YEKHFCFSQPLNDLGDSNVELWVNFFSYDN*RI FFFFQNERG*DWIGKRTLSTRSCNI*LWFSTL*E GIYSLLMAHKCLFKVYHIQQIFMPVYFGEEVKI VQGENNLKHRLGISKHRKSEPVMIMKMSITEHR *FSGQ*NYSV*YHNGEKCHYTFAQIHRIYNTKS* T*CKLWTLGDNDVPM*VHKL*QMYHSDGRCC*W GRLCMCGSRVYLAAVPSLFGSRDQFYGRQFFHR *LGEWGYDLGMKL*NYSTSDQQALVRFS*GTCNL DLLHAQLKIGFALL*ESNATTDLTEGGAQLVML AHPLRGPVSNRPPTGTGPWPGGWGPLGYLCTFC SVFL*TYNRFKIRSIFKKEKRR*PIMILNV*NKN FSMKNSHIKMHNVLRSF*Q*GFKYISYTFQ*FV RQEPSILELCAFVCVCVCVCVCL*YKGYTEWHN DQSHAEIYKCCPTQTPSSSSTVESKFNDSSILLK GCIQKIFT*C*NSRIKTNSKK*E*IVLFIS*KC RAYYQ*GYRSTCLI*NHAILKQLFELFELFQIT HIKCDFINT*YHTI*NLQN*WVTEARACDLSTLE ANRGGLLEARSSTAAWATKQDLVSTTQK*INAW RHSCSPSYSRG*GKRSA*AQEFNTSVG*DCATV LTPA*AAEQDSISKSK*INKTYKILKIK*NMSGL YLPHLN*GLEELNILLQMKHFMHRLAHSK*SIG VNNLCYCYFLESN*QIPQ*MTSQGCNQQDPEYG NFY*INNSISSATIQERERREAIHFKRLKKYMNQ ICMRQSEKLTPTVLRNYLILVW**DCCYVF*KV IIL*IFIIK*L*MK

Frame 1

RAK*YEKYLTRNIKNHQRKCYKTSLRPQEMIEM GFHCVSEDGLDLLTS*SACFCLPKCWDYSREPP RLASSYYFLLCSLFKRAFQHL**ASARLPDNEPT WIERGLLSLPNLLMLQRCEGAHLRKAEPAQYIK TTQLRTPTAEAALGRG*S*TKLCCWGPSL*PP* *APVEVKTLWLTTLPLVV*TCTSFTVPLASTPMN LMEMSSSTWTWRGRRLPGGGLSSANLEVLTRRV H*ETWLWQNTT*TS*LNATTLPLLPMRFLRSQC FPSLP*HWVSPTPSFVLWTTSFLLWSTSHG*AMG SQSQKVFLRPASSPRVIIPSSRSVTSPSSLLLM RFMTARWSTGAWTSLF*NTGSLRFQPLCQSSQR LWSVPWGCLWASWALWWALSSSSKACVQLVLPDT KGHCESHPGREVLPTKTCLG*ATRLPNSSVTSI *NLQGSNKFLL*DLCQIFPSFIQG*LKLWLIIG VLLCFNPI*PHFPDHFSCPVTQKPPSTV*PDNML IS*LTLIFLASLCSHPWHCHPPLNSGNNEINGY RLPLAQNCYSKNFRTKK*VCTNFNVAFKSMTEK *VRIKEI*ISKISKVKMYSQNFKFMKNDDSRSLP LPLSP*DKNSLGRKRNGSQKNIRIRQ*CGCLKR NKYLFLT*G**QWEKGRE*KPQTYLGALNRGAV CLTS*MKLY*ITM*LSLCHSGMKETV**IWLQDV SWKHANIFRNPQPFPLTPPD*GKHELMRETLGR TTQLRQCSSSSGCCVLHWIRHLRGETLTEEMGQ QRPHGSKQL*NP*GYF*PCYLHQ*V*ILIGEKIQ NINAIIDTV*FGAKNPKSKSRTQYFEASILNFI NV*SL*HFWPYFSHPQCRSNNTFLAELLIKFE* F*YLVNALHIVHEHIRKHCGYIYN*FI*KNGSYY MKSTFVFLSLLMI*EIQM*SYG*ISFHMIIEGY FSFSRMREAEIGLVRELLALEVVIFDFGFQLSK REYIPSLWPISVYSRYIIYNRSLCLFTLGKK*R* FKEKII*NTDWESVSTGNLNQ**S*KCPSQSTD DFQGNKTILFDTTMVKNVIIHLLKSTEYITPRV RLNVNYGLWVIMMCQCKFINYSKCTTQMGDVANG GGYACVGAECIWQQSPAFLAAGTSFMEDNFSTD SWGNGGMIWG*NCETIPPQINKH*LDSHKEHAT *ISCMHS*K*GSHSYENLMPPLI*RKAGLSW*CW LTHCVVQFLTGHQLVLVHGPGVGDLWDTSAPSA QFFCEPTTALK*GLFLKRKREGNQL*S*MCKIK ILV*KTVTLKCTTC*EAFSNRVSNIFHIHFNDS* GKNPAFWSCVHLCVCVCVCVCVCNIRGILNGIM TRVMQKSTNAAQLRLLPQAALWKANLMIAVFY* KDVFKKYLRNVKIAELKLILKNKSK*FFLSAKNV GPTINRVTDLPA*FKIMQFLNNYLSYLNYFRLH T*SVTSLILNITLFKIYKISGSQRLVLVILALW RPIGEDCLKPGVQQLPGQQSKTLSLQHKNKLMHG GTPVVPATLEAEARGVLEPRSSILQWAKIVPLC SLQPRQQSRTPSLKVNK*IKLTKF*KSNEICQV CTYHI*TRDLKN*TFYYK*STSCTDWHIVSSR*V LTICVIVIFWSPTNKSHSE*HHRDATNKIQNME TSTR*TTPFLQQQFKREREEKLYILKG*RNI*T KFV*GNQKN*HRLY*GII*F*CGNEIAVMFSKKS LYFRFS**NNYE*

Frame 2

EQNNMKSI*QGI*RIIKENVIKLL*DHKR**RW GFTVLARMVLIS*PRDPPASASQSAGITVVSHR AWPPHIIFYCAVYSRELSSTCDKPQPACRIMSP HGLREASYPCQTY*CYRDVREPT*EKLNLPST*K PLSSEHQLLRLPWEEDDPKQSSAAGGPRSDHRD EPLWR*RHCG*PRCLLWCKLVPVLRSLWPVHP* I*WR*AVLRGPGEEGDCLAVA*VQQIWRF*PAGC TEKHGCGKTQLEHHD*TLQLYRCYQ*GS*GHSV FQVSRDTGSAQHPHLSCGQHLSSCGQHHMAEQW AVSHRRCF*DQLPLQE*SFLLQDQLPHLPPFC** DL*LQGGALGPGPASSETLGA*DSSPYVRAHRD CGLCPGVVCGPHGHCGGHCLHHPRPAFSWCFQT PRAIVNPILEGKCYLQRHAWGKPPGYLIPQ*PPS KISKEAINSFYEIYVKFFHLSSRAD*NYG**LG YSYVSIQFNLISQIIFHVQ*HRSHQVQYSLIIC *FLS*H*YFLLPCVPTLGTATHPSIQATMKLMDT VCPWPRIVIAKILEPKNKSVLISMWLLKV*QRN KLG*RKFESQKYQK*KCILKTLNL*RMMTVEAF LFPCHLEIKIL*AGKEMEVRKTLE*DNNVGV*KG TNTYSSHRVSDNGKRDGSRSHRHI*EP*IEAQS ASPPE*SCTR*PCSFPCATLA*RRQYSEYGYRM FLGNMPISSEIPSPFP*PLLTKESMSL*EKP*EE QHS*DNVAAAVGAVSSTGFAISEEKLSQRKWVS SDLMALNSYEIHEDISNHATCISEFKF*LEKKY KTLMQ*LIQYSLVQRTLNPNPGLSTLKLVF*TL* MCEVSNISGLTFLIHNVGVIILSLQSY**NLNN FDI*SMPYT*YMNT*ENIVVIFIINLFKRMDHI I*KALLFFSAS**FRRFKCRAMGEFLFI**LKDI FLFPE*ERLRLDW*ENS*H*KL*YLTLVFNSLR GNIFPPYGP*VFIQGISYTTDLYACLLWGRSED SSRRK*FKTQTGNQ*AQEI*TSDDHENVHHRAQM IFRAIKLFCLIPQW*KMSLYICSNPQNI*HQEL DLM*TMDFG***CANVSS*IIANVPLRWEMLLM GEAMHVWEQSVSGSSPQPFWQQGPVLWKTIFPQI VGGMGV*SGDETVKLFHLRSTSIS*ILIRNMQP RSLACTVKNRVRTPMRI*CHH*SDGRRGSVGNA GSPTAWSSF*QATNWYWSMARGLGTSGIPLHLLL SFSVNLQPL*NKVYF*KGKEKVTNYDPKCVK*K F*YEKQSH*NAQRAKKLLAIGFQIYFIYISMIR EARTQHFGVVCICVCVCVCVCVSVI*GVY*MA** PESCRNLQMLPNSDSFLKQHCGKQI***QYFIK RMYSKNIYVMLK*QN*N*F*KIRVNSSFYQLKM *GLLSIGLQIYLPDLKSCNS*TII*AI*IISDYT HKV*LH*YLISHYLKFTKLVGHRGSCL*S*HFG GQ*GRIA*SQEFNSCLGNKARPCLYNTKIN*CM AALL*SQLL*RLRQEECLSPGVQYFSGLRLCHCA HSSLGSRAGLHL*K*INK*NLQNFKNQMKYVRF VPTTFKLGT*RIKHFITNEALHAQTGT**VVDR C*QFVLLLFSGVQLTNPTVNDITGMQPTRSRIWK LLLDKQLHFFSNNSREREKRSYTF*KAEEIYEP NLYEAIRKTDTDCIKELSNFSVVMRLLLCFLKS HYTLDFHNKIIMNE

Sequence HLA-DQA1.bAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCT GAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCA TCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAAT GGACTTGCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGC TGCTATATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTG GGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCAT CTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGT AACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGC CACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCT GTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCA AAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAG AAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGA AGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTC TGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCT GACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTC GCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTA CCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAA TCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTA ATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGG TTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAG AGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCAC TAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAA CAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTG AACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTA TACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAA TTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTG GCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGA TCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCC ACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGC CCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAA GAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAA TAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAG CACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCT AAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTC TTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTA GTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAG CAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCC AGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAA AACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAG CACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAA TGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAG AGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATT TTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F3 (forward primer)
HLA-DQA1.R3 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVADHVASCGVNLY QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWEP EIPAPMSELTETVVCALGLSVGLMGIVVGTVFI IQGLRSVGASRHQGPL*IPSWKGRCIAIYRSRRM DLLNDLALFSGPIYHIPFLLQIFLLSPFLWDLS CYIPSELTNAFTFFP*PPDFFFLFSNVTYKDMP GVSHPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSI FHPGLTETMANNWGTLMFQSNLTSFPRSFFMSS NTEATKYSIA**YVDFLADINISCFLVFPPLAL PPTPQFRQQ*N*WIPSALGPELL*QKF*NQKISL Y*FQCGF*KYDREIS*DKGNLNLKNIKSKNVFS KL*IYEE**Q*KPSSSLVTLR*KFFRQEKKWKS EKH*NKTIMWVSEKEQILIPHIGLVTMGKGTGVE ATDISRSPE*RRSLPHLLNEAVLDNHVAFPVPL WHEGDSIVNMATGCFLETCQYLQKSPALSPNPS *LRKA*AYERNPRKNNTVETM*QQQWVLCPPLDS PSPRRNSHRGNGSAATSWL*TAMKSMRIFLTML PASVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQ IQDSVL*S*YFKLYKCVKSLTFLALLFSSTM*E* *YFPCRVIDKI*IILIFSQCLTHST*THKKTLW LYL*LIYLKEWIILYEKHFCFSQPLNDLGDSNV ELWVNFFSYDN*RIFFFFQNERG*DWIGKRTLST RSCNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQ QIFMPVYFGEEVKIVQGENNLKHRLGISKHRKS EPVMIMKMSITEHR*FSGQ*NYSV*YHNGEKCHY TFAQIHRIYNTKS*T*CKLWTLGDNDVPM*VHK L*QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLF GSRDQFYGRQFFHR*LGEWGYDLGMKL*NYSTSD QQALVRFS*GTCNLDLLHAQLKIGFALL*ESNA TTDLTEGGAQLVMLAHPLRGPVSNRPPTGTGPW PGGWGPLGYLCTFCSVFL*TYNRFKIRSIFKKEK RR*PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q *GFKYISYTFQ*FVRQEPSILELCAFVCVCVCV CVCL*YKGYTEWHNDQSHAEIYKCCPTQTPSSSS TVESKFNDSSILLKGCIQKIFT*C*NSRIKTNS KK*E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAI LKQLFELFELFQITHIKCDFINT*YHTI*NLQN* WVTEARACDLSTLEANRGGLLEARSSTAAWATK QDLVSTTQK*INAWRHSCSPSYSRG*GKRSA*A QEFNTSVG*DCATVLTPA*AAEQDSISKSK*INK TYKILKIK*NMSGLYLPHLN*GLEELNILLQMK HFMHRLAHSK*SIGVNNLCYCYFLESN*QIPQ* MTSQGCNQQDPEYGNFY*INNSISSATIQERERR EAIHFKRLKKYMNQICMRQSEKLTPTVLRNYLI LVW**DCCYVF*KVIIL*IFIIK*L*MK

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGSL RFQPLCQSSQRLWSVPWGCLWASWALWWALSSS SKACVQLVLPDTKGHCESHPGREGASPSTGAEE WTC*MT*HYSLARFIISLFSSKYFSSHLFSGT*A AISPQSSQMPLHSFPDLLIFFFFSQMLPTKTCL G*ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFP SFIQG*LKLWLIIGVLLCFNPI*PHFPDHFSCPV TQKPPSTV*PDNMLIS*LTLIFLASLCSHPWHC HPPLNSGNNEINGYRLPLAQNCYSKNFRTKK*V CTNFNVAFKSMTEK*VRIKEI*ISKISKVKMYSQ NFKFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQ KNIRIRQ*CGCLKRNKYLFLT*G**QWEKGRE* KPQTYLGALNRGAVCLTS*MKLY*ITM*LSLCHS GMKETV**IWLQDVSWKHANIFRNPQPFPLTPP D*GKHELMRETLGRTTQLRQCSSSSGCCVLHWI RHLRGETLTEEMGQQRPHGSKQL*NP*GYF*PCY LHQ*V*ILIGEKIQNINAIIDTV*FGAKNPKSK SRTQYFEASILNFINV*SL*HFWPYFSHPQCRS NNTFLAELLIKFE*F*YLVNALHIVHEHIRKHCG YIYN*FI*KNGSYYMKSTFVFLSLLMI*EIQM* SYG*ISFHMIIEGYFSFSRMREAEIGLVRELLA LEVVIFDFGFQLSKREYIPSLWPISVYSRYIIYN RSLCLFTLGKK*R*FKEKII*NTDWESVSTGNL NQ**S*KCPSQSTDDFQGNKTILFDTTMVKNVI IHLLKSTEYITPRVRLNVNYGLWVIMMCQCKFIN YSKCTTQMGDVANGGGYACVGAECIWQQSPAFL AAGTSFMEDNFSTDSWGNGGMIWG*NCETIPPQ INKH*LDSHKEHAT*ISCMHS*K*GSHSYENLMP PLI*RKAGLSW*CWLTHCVVQFLTGHQLVLVHG PGVGDLWDTSAPSAQFFCEPTTALK*GLFLKRK REGNQL*S*MCKIKILV*KTVTLKCTTC*EAFSN RVSNIFHIHFNDS*GKNPAFWSCVHLCVCVCVC VCVCNIRGILNGIMTRVMQKSTNAAQLRLLPQA ALWKANLMIAVFY*KDVFKKYLRNVKIAELKLIL KNKSK*FFLSAKNVGPTINRVTDLPA*FKIMQF LNNYLSYLNYFRLHT*SVTSLILNITLFKIYKI SGSQRLVLVILALWRPIGEDCLKPGVQQLPGQQS KTLSLQHKNKLMHGGTPVVPATLEAEARGVLEP RSSILQWAKIVPLCSLQPRQQSRTPSLKVNK*I KLTKF*KSNEICQVCTYHI*TRDLKN*TFYYK*S TSCTDWHIVSSR*VLTICVIVIFWSPTNKSHSE *HHRDATNKIQNMETSTR*TTPFLQQQFKRERE EKLYILKG*RNI*TKFV*GNQKN*HRLY*GII*F *CGNEIAVMFSKKSLYFRFS**NNYE*

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA* VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ DQLPHLPPFC**DL*LQGGALGPGPASSETLGA *DSSPYVRAHRDCGLCPGVVCGPHGHCGGHCLHH PRPAFSWCFQTPRAIVNPILEGKVHRHLQEQKN GLAK*PSTILWPDLSYPFSPPNISPLTFSLGLK LLYPLRAHKCLYILSLTS*FFFSFLKCYLQRHAW GKPPGYLIPQ*PPSKISKEAINSFYEIYVKFFH LSSRAD*NYG**LGYSYVSIQFNLISQIIFHVQ *HRSHQVQYSLIIC*FLS*H*YFLLPCVPTLGTA THPSIQATMKLMDTVCPWPRIVIAKILEPKNKS VLISMWLLKV*QRNKLG*RKFESQKYQK*KCIL KTLNL*RMMTVEAFLFPCHLEIKIL*AGKEMEVR KTLE*DNNVGV*KGTNTYSSHRVSDNGKRDGSR SHRHI*EP*IEAQSASPPE*SCTR*PCSFPCAT LA*RRQYSEYGYRMFLGNMPISSEIPSPFP*PLL TKESMSL*EKP*EEQHS*DNVAAAVGAVSSTGF AISEEKLSQRKWVSSDLMALNSYEIHEDISNHA TCISEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPN PGLSTLKLVF*TL*MCEVSNISGLTFLIHNVGV IILSLQSY**NLNNFDI*SMPYT*YMNT*ENIV VIFIINLFKRMDHII*KALLFFSAS**FRRFKCR AMGEFLFI**LKDIFLFPE*ERLRLDW*ENS*H *KL*YLTLVFNSLRGNIFPPYGP*VFIQGISYT TDLYACLLWGRSEDSSRRK*FKTQTGNQ*AQEI* TSDDHENVHHRAQMIFRAIKLFCLIPQW*KMSL YICSNPQNI*HQELDLM*TMDFG***CANVSS* IIANVPLRWEMLLMGEAMHVWEQSVSGSSPQPFW QQGPVLWKTIFPQIVGGMGV*SGDETVKLFHLR STSIS*ILIRNMQPRSLACTVKNRVRTPMRI*C HH*SDGRRGSVGNAGSPTAWSSF*QATNWYWSMA RGLGTSGIPLHLLLSFSVNLQPL*NKVYF*KGK EKVTNYDPKCVK*KF*YEKQSH*NAQRAKKLLA IGFQIYFIYISMIREARTQHFGVVCICVCVCVCV CVSVI*GVY*MA**PESCRNLQMLPNSDSFLKQ HCGKQI***QYFIKRMYSKNIYVMLK*QN*N*F *KIRVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS *TII*AI*IISDYTHKV*LH*YLISHYLKFTKL VGHRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNK ARPCLYNTKIN*CMAALL*SQLL*RLRQEECLSP GVQYFSGLRLCHCAHSSLGSRAGLHL*K*INK* NLQNFKNQMKYVRFVPTTFKLGT*RIKHFITNE ALHAQTGT**VVDRC*QFVLLLFSGVQLTNPTVN DITGMQPTRSRIWKLLLDKQLHFFSNNSREREK RSYTF*KAEEIYEPNLYEAIRKTDTDCIKELSN FSVVMRLLLCFLKSHYTLDFHNKIIMNE

Sequence HLA-DQA1.eAug05

GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCC AGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGGATCCATCTCTGACTCCCTGCTCCTTTATAGAGATG GACAGTGGGTTTGTAAAACAAAAGTTGAAAAGTCAGATAGTTAAAAGGGGAAGTGAACTGGAAGGTACTTTAAACTTTCACAACTTTATTAACCGTGGCT GCTCCCATTATGATTTTGTTCGGCAGTGGAAGTTTCACCTGCTTCTCCAGAGCGCTTGGCGTCTTTGTTCCAAATTTCCTTTCTTCAACCTCACACCAGA GTGCCCTGGTCAGGCTTGGCTCATTCATTAGGCACAATGTGGGCAGTGCAGGGGACCCTCCATACTGTAAAGCCACATGAGAAGGTTTTAACTCCTTTTA AAATTAGAAAAATAATGGAATATTAGAGCCTAAGAAAATGTTTTAACTTTTAATTCAGCCTAGATTATATTGTCTTTATACCAATTCAGTCATAAAATAT AATTTTCCATATTTTTGTGGAGGAAGAGGCCCATACAAGCAAGAATGCTCGGGGCTCACATGTCAGAATGCAGCCTTGGTCATGACTGATCCTGGCCTTC GTATGGTTCTGCTACCTGTGTGCCTGCCCGTCTTCCCCAAAATCTATGTGGTCCTCGAATATAGCAACTGTCATTCAATACACATGTTTGAGCACACAGT GAGCTAAGTTTTAAGGATTCAAAGATGAAAAGTCATGCTGTCTTCCCTGCAGAGGGTGCTCAGATTAGTGATGGAAACAGTATGGGGTGCAGGAAAGCAG AAGGCCATTGCTGAGCAGGGCAGTGGACCCAGCAGAGGCTGAAACAATACAAGTGACTTGGTTCCAGGTGGGCCAGCAGGGTGACGTCCTCTAGCAAAAT TTGGCACCCAAGACAAGTACCAGAAGAAAAGAAAGACTGCATGTATTCCACATATATTCATGTTTGAACAAGGAGTCAAGATTTATGGCAAGGATAAGGA GGCTTTGTTGGTGACCTGTTAAGACCAACCAGGGCAGTCATGCTGGATAGGGAAGAAGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTT ACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAG CAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCT ACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTG TGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAG TTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATT CCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAG GCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAATGGACTT GCTAAATGACCTAGCACTATTCTCTGGCCCGATTTATCATATCCCTTTTCTCCTCCAAATATTTCTCCTCTCACCTTTTCTCTGGGACTTAAGCTGCTAT ATCCCCTCAGAGCTCACAAATGCCTTTACATTCTTTCCCTGACCTCCTGATTTTTTTTTTCTTTTCTCAAATGTTACCTACAAAGACATGCCTGGGGTAA GCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCA TCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACA GAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCA CCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTA ATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTT TAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAAC ATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCAC AGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCAT GAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAA GGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATC TCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCA TCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGG ACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATA CTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATAT TTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTAT GGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAG TTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATC TTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAG TGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATT TGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAG CAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCA GGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACA AGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACT GATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGG GTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAG GTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGT TTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGT GTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGT GGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAA TAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAAC AATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGT CACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGAC CTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGT TCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTA CAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTC ATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACAT CACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGC TATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTG TGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA

HLA-DQA1.F3 (forward primer)
HLA-DQA1.R3 (reverse primer)
Alternative acceptor

Frame 0

EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSF QGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA LLLGALALTTVMSPCGGEDIVGSISDSLLLYRD GQWVCKTKVEKSDS*KGK*TGRYFKLSQLY*PWL LPL*FCSAVEVSPASPERLASLFQISFLQPHTR VPWSGLAHSLGTMWAVQGTLHTVKPHEKVLTPF KIRKIMEY*SLRKCFNF*FSLDYIVFIPIQS*NI IFHIFVEEEAHTSKNARGSHVRMQPWS*LILAF VWFCYLCACPSSPKSMWSSNIATVIQYTCLSTQ *AKF*GFKDEKSCCLPCRGCSD**WKQYGVQESR RPLLSRAVDPAEAETIQVTWFQVGQQGDVL*QN LAPKTSTRRKERLHVFHIYSCLNKESRFMARIR RLCW*PVKTNQGSHAG*GRS*PRCLLWCKLVPVL RSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*VQ QIWRF*PAGCTEKHGCGKTQLEHHD*TLQLYRC YQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLSSC GQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQDQ LPHLPPFC**DL*LQGGALGPGPASSETLGA*D SSPYVRAHRDCGLCPGVVCGPHGHCGGHCLHHPR PAFSWCFQTPRAIVNPILEGKVHRHLQEQKNGL AK*PSTILWPDLSYPFSPPNISPLTFSLGLKLL YPLRAHKCLYILSLTS*FFFSFLKCYLQRHAWGK PPGYLIPQ*PPSKISKEAINSFYEIYVKFFHLS SRAD*NYG**LGYSYVSIQFNLISQIIFHVQ*H RSHQVQYSLIIC*FLS*H*YFLLPCVPTLGTATH PSIQATMKLMDTVCPWPRIVIAKILEPKNKSVL ISMWLLKV*QRNKLG*RKFESQKYQK*KCILKT LNL*RMMTVEAFLFPCHLEIKIL*AGKEMEVRKT LE*DNNVGV*KGTNTYSSHRVSDNGKRDGSRSH RHI*EP*IEAQSASPPE*SCTR*PCSFPCATLA *RRQYSEYGYRMFLGNMPISSEIPSPFP*PLLTK ESMSL*EKP*EEQHS*DNVAAAVGAVSSTGFAI SEEKLSQRKWVSSDLMALNSYEIHEDISNHATC ISEFKF*LEKKYKTLMQ*LIQYSLVQRTLNPNPG LSTLKLVF*TL*MCEVSNISGLTFLIHNVGVII LSLQSY**NLNNFDI*SMPYT*YMNT*ENIVVI FIINLFKRMDHII*KALLFFSAS**FRRFKCRAM GEFLFI**LKDIFLFPE*ERLRLDW*ENS*H*K L*YLTLVFNSLRGNIFPPYGP*VFIQGISYTTD LYACLLWGRSEDSSRRK*FKTQTGNQ*AQEI*TS DDHENVHHRAQMIFRAIKLFCLIPQW*KMSLYI CSNPQNI*HQELDLM*TMDFG***CANVSS*II ANVPLRWEMLLMGEAMHVWEQSVSGSSPQPFWQQ GPVLWKTIFPQIVGGMGV*SGDETVKLFHLRST SIS*ILIRNMQPRSLACTVKNRVRTPMRI*CHH *SDGRRGSVGNAGSPTAWSSF*QATNWYWSMARG LGTSGIPLHLLLSFSVNLQPL*NKVYF*KGKEK VTNYDPKCVK*KF*YEKQSH*NAQRAKKLLAIG FQIYFIYISMIREARTQHFGVVCICVCVCVCVCV SVI*GVY*MA**PESCRNLQMLPNSDSFLKQHC GKQI***QYFIKRMYSKNIYVMLK*QN*N*F*K IRVNSSFYQLKM*GLLSIGLQIYLPDLKSCNS*T II*AI*IISDYTHKV*LH*YLISHYLKFTKLVG HRGSCL*S*HFGGQ*GRIA*SQEFNSCLGNKAR PCLYNTKIN*CMAALL*SQLL*RLRQEECLSPGV QYFSGLRLCHCAHSSLGSRAGLHL*K*INK*NL QNFKNQMKYVRFVPTTFKLGT*RIKHFITNEAL HAQTGT**VVDRC*QFVLLLFSGVQLTNPTVNDI TGMQPTRSRIWKLLLDKQLHFFSNNSREREKRS YTF*KAEEIYEPNLYEAIRKTDTDCIKELSNFS VVMRLLLCFLKSHYTLDFHNKIIMNE

Frame 1

KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPS RALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL CCWGPSL*PP**APVEVKTLWDPSLTPCSFIEM DSGFVKQKLKSQIVKRGSELEGTLNFHNFINRG CSHYDFVRQWKFHLLLQSAWRLCSKFPFFNLTPE CPGQAWLIH*AQCGQCRGPSIL*SHMRRF*LLL KLEK*WNIRA*ENVLTFNSA*IILSLYQFSHKI *FSIFLWRKRPIQARMLGAHMSECSLGHD*SWPS YGSATCVPARLPQNLCGPRI*QLSFNTHV*AHS ELSFKDSKMKSHAVFPAEGAQISDGNSMGCRKA EGHC*AGQWTQQRLKQYK*LGSRWASRVTSSSKI WHPRQVPEEKKDCMYSTYIHV*TRSQDLWQG*G GFVGDLLRPTRAVMLDREEADHVASCGVNLYQF YGPSGQYTHEFDGDEQFYVDLERKETAWRWPEFS KFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAA TNEVPEVTVFSKSPVTLGQPNTLICLVDNIFPP VVNITWLSNGQSVTEGVSETSFLSKSDHSFFKIS YLTFLPSADEIYDCKVEHWGLDQPLLKHWEPEI PAPMSELTETVVCALGLSVGLMGIVVGTVFIIQ GLRSVGASRHQGPL*IPSWKGRCIAIYRSRRMDL LNDLALFSGPIYHIPFLLQIFLLSPFLWDLSCY IPSELTNAFTFFP*PPDFFFLFSNVTYKDMPGV SHPAT*FLSNLHLKSPRKQ*IPFMRSMSNFSIFH PGLTETMANNWGTLMFQSNLTSFPRSFFMSSNT EATKYSIA**YVDFLADINISCFLVFPPLALPP TPQFRQQ*N*WIPSALGPELL*QKF*NQKISLY* FQCGF*KYDREIS*DKGNLNLKNIKSKNVFSKL *IYEE**Q*KPSSSLVTLR*KFFRQEKKWKSEK H*NKTIMWVSEKEQILIPHIGLVTMGKGTGVEAT DISRSPE*RRSLPHLLNEAVLDNHVAFPVPLWH EGDSIVNMATGCFLETCQYLQKSPALSPNPS*L RKA*AYERNPRKNNTVETM*QQQWVLCPPLDSPS PRRNSHRGNGSAATSWL*TAMKSMRIFLTMLPA SVSLNFNWRKNTKH*CNN*YSIVWCKEP*IQIQ DSVL*S*YFKLYKCVKSLTFLALLFSSTM*E**Y FPCRVIDKI*IILIFSQCLTHST*THKKTLWLY L*LIYLKEWIILYEKHFCFSQPLNDLGDSNVEL WVNFFSYDN*RIFFFFQNERG*DWIGKRTLSTRS CNI*LWFSTL*EGIYSLLMAHKCLFKVYHIQQI FMPVYFGEEVKIVQGENNLKHRLGISKHRKSEP VMIMKMSITEHR*FSGQ*NYSV*YHNGEKCHYTF AQIHRIYNTKS*T*CKLWTLGDNDVPM*VHKL* QMYHSDGRCC*WGRLCMCGSRVYLAAVPSLFGS RDQFYGRQFFHR*LGEWGYDLGMKL*NYSTSDQQ ALVRFS*GTCNLDLLHAQLKIGFALL*ESNATT DLTEGGAQLVMLAHPLRGPVSNRPPTGTGPWPG GWGPLGYLCTFCSVFL*TYNRFKIRSIFKKEKRR *PIMILNV*NKNFSMKNSHIKMHNVLRSF*Q*G FKYISYTFQ*FVRQEPSILELCAFVCVCVCVCV CL*YKGYTEWHNDQSHAEIYKCCPTQTPSSSSTV ESKFNDSSILLKGCIQKIFT*C*NSRIKTNSKK *E*IVLFIS*KCRAYYQ*GYRSTCLI*NHAILK QLFELFELFQITHIKCDFINT*YHTI*NLQN*WV TEARACDLSTLEANRGGLLEARSSTAAWATKQD LVSTTQK*INAWRHSCSPSYSRG*GKRSA*AQE FNTSVG*DCATVLTPA*AAEQDSISKSK*INKTY KILKIK*NMSGLYLPHLN*GLEELNILLQMKHF MHRLAHSK*SIGVNNLCYCYFLESN*QIPQ*MT SQGCNQQDPEYGNFY*INNSISSATIQERERREA IHFKRLKKYMNQICMRQSEKLTPTVLRNYLILV W**DCCYVF*KVIIL*IFIIK*L*MK

Frame 2

RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLP GLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS SAAGGPRSDHRDEPLWR*RHCGIHL*LPAPL*RW TVGL*NKS*KVR*LKGEVNWKVL*TFTTLLTVA APIMILFGSGSFTCFSRALGVFVPNFLSSTSHQ SALVRLGSFIRHNVGSAGDPPYCKAT*EGFNSF* N*KNNGILEPKKMF*LLIQPRLYCLYTNSVIKY NFPYFCGGRGPYKQECSGLTCQNAALVMTDPGL RMVLLPVCLPVFPKIYVVLEYSNCHSIHMFEHTV S*VLRIQR*KVMLSSLQRVLRLVMETVWGAGKQ KAIAEQGSGPSRG*NNTSDLVPGGPAG*RPLAK FGTQDKYQKKRKTACIPHIFMFEQGVKIYGKDKE ALLVTC*DQPGQSCWIGKKLTTLPLVV*TCTSF TVPLASTPMNLMEMSSSTWTWRGRRLPGGGLSS ANLEVLTRRVH*ETWLWQNTT*TS*LNATTLPLL PMRFLRSQCFPSLP*HWVSPTPSFVLWTTSFLL WSTSHG*AMGSQSQKVFLRPASSPRVIIPSSRS VTSPSSLLLMRFMTARWSTGAWTSLF*NTGSLRF QPLCQSSQRLWSVPWGCLWASWALWWALSSSSK ACVQLVLPDTKGHCESHPGREGASPSTGAEEWT C*MT*HYSLARFIISLFSSKYFSSHLFSGT*AAI SPQSSQMPLHSFPDLLIFFFFSQMLPTKTCLG* ATRLPNSSVTSI*NLQGSNKFLL*DLCQIFPSF IQG*LKLWLIIGVLLCFNPI*PHFPDHFSCPVTQ KPPSTV*PDNMLIS*LTLIFLASLCSHPWHCHP PLNSGNNEINGYRLPLAQNCYSKNFRTKK*VCT NFNVAFKSMTEK*VRIKEI*ISKISKVKMYSQNF KFMKNDDSRSLPLPLSP*DKNSLGRKRNGSQKN IRIRQ*CGCLKRNKYLFLT*G**QWEKGRE*KP QTYLGALNRGAVCLTS*MKLY*ITM*LSLCHSGM KETV**IWLQDVSWKHANIFRNPQPFPLTPPD* GKHELMRETLGRTTQLRQCSSSSGCCVLHWIRH LRGETLTEEMGQQRPHGSKQL*NP*GYF*PCYLH Q*V*ILIGEKIQNINAIIDTV*FGAKNPKSKSR TQYFEASILNFINV*SL*HFWPYFSHPQCRSNN TFLAELLIKFE*F*YLVNALHIVHEHIRKHCGYI YN*FI*KNGSYYMKSTFVFLSLLMI*EIQM*SY G*ISFHMIIEGYFSFSRMREAEIGLVRELLALE VVIFDFGFQLSKREYIPSLWPISVYSRYIIYNRS LCLFTLGKK*R*FKEKII*NTDWESVSTGNLNQ **S*KCPSQSTDDFQGNKTILFDTTMVKNVIIH LLKSTEYITPRVRLNVNYGLWVIMMCQCKFINYS KCTTQMGDVANGGGYACVGAECIWQQSPAFLAA GTSFMEDNFSTDSWGNGGMIWG*NCETIPPQIN KH*LDSHKEHAT*ISCMHS*K*GSHSYENLMPPL I*RKAGLSW*CWLTHCVVQFLTGHQLVLVHGPG VGDLWDTSAPSAQFFCEPTTALK*GLFLKRKRE GNQL*S*MCKIKILV*KTVTLKCTTC*EAFSNRV SNIFHIHFNDS*GKNPAFWSCVHLCVCVCVCVC VCNIRGILNGIMTRVMQKSTNAAQLRLLPQAAL WKANLMIAVFY*KDVFKKYLRNVKIAELKLILKN KSK*FFLSAKNVGPTINRVTDLPA*FKIMQFLN NYLSYLNYFRLHT*SVTSLILNITLFKIYKISG SQRLVLVILALWRPIGEDCLKPGVQQLPGQQSKT LSLQHKNKLMHGGTPVVPATLEAEARGVLEPRS SILQWAKIVPLCSLQPRQQSRTPSLKVNK*IKL TKF*KSNEICQVCTYHI*TRDLKN*TFYYK*STS CTDWHIVSSR*VLTICVIVIFWSPTNKSHSE*H HRDATNKIQNMETSTR*TTPFLQQQFKREREEK LYILKG*RNI*TKFV*GNQKN*HRLY*GII*F*C GNEIAVMFSKKSLYFRFS**NNYE*