Back to Rnomics Platform
Open Session
×
1 warning:
- Access restricted to public data.
Please sign in at Rnomics Platform to access your data.
Sequence HLA-DQA1.gAug05
GAAGATTCCAATTTAATGTCCTCCCTTTATTCATAGAACAATTCCTCAAGTCCACTCTGAGTAGAGGCTGCATCACAAGGGGATTGCCCCGTCTCCTTCCAGGGCTCTTAATACAAACTCTTCAACTAGTAACTGAGATGTCACCATAGGGGATTTTTCTAATTGGCCAAAACCTGACTTGGCAGGGTTTGGTTTGGGTG
TCTTCAGATTTCCTTGTCTTGAGGTCCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCT
CTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACC
AGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGA
GTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACC
GCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTC
CTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAA
GATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGTGTTA
CCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATCTAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATC
TATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGGTACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGA
TCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTTGATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGT
TCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATACCGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTA
GAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAATAAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAA
AGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCCTCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGG
AAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGGAACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGG
AAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCTGCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTA
GCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGTTTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCC
TTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGAACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCT
GTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGCAGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGG
ATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACAAAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCA
AAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATAAATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTC
ATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAATTTTGATATTTAGTCAATGCCTTACACATAGTACATGAACA
CATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTATATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATT
TAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATATTTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGG
TAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGAGGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTC
AAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATAGTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCA
GTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGATGATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAA
TGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTAGACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTG
CCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGGGGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAG
CAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGATAGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTG
AAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCTAGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTC
CTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTGGCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAA
CTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCTCAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTC
TATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAATTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTG
CTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTGAGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATGACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCA
GACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAAAGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGC
AGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGTAGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGA
TTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACACACATAAAGTGTGACTTCATTAATACTTAATATCACACTAT
TTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGAGGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACA
GCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGGCGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCA
AGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGCTCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAA
AAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTTGTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAAC
ATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGTGTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACT
AACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAAACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAAT
TCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCAAATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTA
TTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTCATTATACTTTAGATTTTCATAATAAAATAATTATGAATGA
AA
- HLA-DQA1.F3 (forward primer)
- HLA-DQA1.R3 (reverse primer)
- Single exon skipping
Frame 0
EDSNLMSSLYS*NNSSSPL*VEAASQGDCPVSFQGS*YKLFN**LRCHHRGFF*LAKT*LGRVWFGC
LQISLS*GPHNYSTAQNTNC*GCLGKRMILNKA
LLLGALALTTVMSPCGGEDIVADHVASCGVNLY
QFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE
FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNST
AATNEVPEVTVFSKSPVTLGQPNTLICLVDNIF
PPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFK
ISYLTFLPSADEIYDCKVEHWGLDQPLLKHWVL
PTKTCLG*ATRLPNSSVTSI*NLQGSNKFLL*D
LCQIFPSFIQG*LKLWLIIGVLLCFNPI*PHFPD
HFSCPVTQKPPSTV*PDNMLIS*LTLIFLASLC
SHPWHCHPPLNSGNNEINGYRLPLAQNCYSKNF
RTKK*VCTNFNVAFKSMTEK*VRIKEI*ISKISK
VKMYSQNFKFMKNDDSRSLPLPLSP*DKNSLGR
KRNGSQKNIRIRQ*CGCLKRNKYLFLT*G**QW
EKGRE*KPQTYLGALNRGAVCLTS*MKLY*ITM*
LSLCHSGMKETV**IWLQDVSWKHANIFRNPQP
FPLTPPD*GKHELMRETLGRTTQLRQCSSSSGC
CVLHWIRHLRGETLTEEMGQQRPHGSKQL*NP*G
YF*PCYLHQ*V*ILIGEKIQNINAIIDTV*FGA
KNPKSKSRTQYFEASILNFINV*SL*HFWPYFS
HPQCRSNNTFLAELLIKFE*F*YLVNALHIVHEH
IRKHCGYIYN*FI*KNGSYYMKSTFVFLSLLMI
*EIQM*SYG*ISFHMIIEGYFSFSRMREAEIGL
VRELLALEVVIFDFGFQLSKREYIPSLWPISVYS
RYIIYNRSLCLFTLGKK*R*FKEKII*NTDWES
VSTGNLNQ**S*KCPSQSTDDFQGNKTILFDTT
MVKNVIIHLLKSTEYITPRVRLNVNYGLWVIMMC
QCKFINYSKCTTQMGDVANGGGYACVGAECIWQ
QSPAFLAAGTSFMEDNFSTDSWGNGGMIWG*NC
ETIPPQINKH*LDSHKEHAT*ISCMHS*K*GSHS
YENLMPPLI*RKAGLSW*CWLTHCVVQFLTGHQ
LVLVHGPGVGDLWDTSAPSAQFFCEPTTALK*G
LFLKRKREGNQL*S*MCKIKILV*KTVTLKCTTC
*EAFSNRVSNIFHIHFNDS*GKNPAFWSCVHLC
VCVCVCVCVCNIRGILNGIMTRVMQKSTNAAQL
RLLPQAALWKANLMIAVFY*KDVFKKYLRNVKIA
ELKLILKNKSK*FFLSAKNVGPTINRVTDLPA*
FKIMQFLNNYLSYLNYFRLHT*SVTSLILNITL
FKIYKISGSQRLVLVILALWRPIGEDCLKPGVQQ
LPGQQSKTLSLQHKNKLMHGGTPVVPATLEAEA
RGVLEPRSSILQWAKIVPLCSLQPRQQSRTPSL
KVNK*IKLTKF*KSNEICQVCTYHI*TRDLKN*T
FYYK*STSCTDWHIVSSR*VLTICVIVIFWSPT
NKSHSE*HHRDATNKIQNMETSTR*TTPFLQQQ
FKREREEKLYILKG*RNI*TKFV*GNQKN*HRLY
*GII*F*CGNEIAVMFSKKSLYFRFS**NNYE*
Frame 1
KIPI*CPPFIHRTIPQVHSE*RLHHKGIAPSPSRALNTNSSTSN*DVTIGDFSNWPKPDLAGFGLG
VFRFPCLEVLTITLQLRTPTAEAALGRG*S*TKL
CCWGPSL*PP**APVEVKTLWLTTLPLVV*TCT
SFTVPLASTPMNLMEMSSSTWTWRGRRLPGGGL
SSANLEVLTRRVH*ETWLWQNTT*TS*LNATTLP
LLPMRFLRSQCFPSLP*HWVSPTPSFVLWTTSF
LLWSTSHG*AMGSQSQKVFLRPASSPRVIIPSS
RSVTSPSSLLLMRFMTARWSTGAWTSLF*NTGCY
LQRHAWGKPPGYLIPQ*PPSKISKEAINSFYEI
YVKFFHLSSRAD*NYG**LGYSYVSIQFNLISQ
IIFHVQ*HRSHQVQYSLIIC*FLS*H*YFLLPCV
PTLGTATHPSIQATMKLMDTVCPWPRIVIAKIL
EPKNKSVLISMWLLKV*QRNKLG*RKFESQKYQ
K*KCILKTLNL*RMMTVEAFLFPCHLEIKIL*AG
KEMEVRKTLE*DNNVGV*KGTNTYSSHRVSDNG
KRDGSRSHRHI*EP*IEAQSASPPE*SCTR*PC
SFPCATLA*RRQYSEYGYRMFLGNMPISSEIPSP
FP*PLLTKESMSL*EKP*EEQHS*DNVAAAVGA
VSSTGFAISEEKLSQRKWVSSDLMALNSYEIHE
DISNHATCISEFKF*LEKKYKTLMQ*LIQYSLVQ
RTLNPNPGLSTLKLVF*TL*MCEVSNISGLTFL
IHNVGVIILSLQSY**NLNNFDI*SMPYT*YMN
T*ENIVVIFIINLFKRMDHII*KALLFFSAS**F
RRFKCRAMGEFLFI**LKDIFLFPE*ERLRLDW
*ENS*H*KL*YLTLVFNSLRGNIFPPYGP*VFI
QGISYTTDLYACLLWGRSEDSSRRK*FKTQTGNQ
*AQEI*TSDDHENVHHRAQMIFRAIKLFCLIPQ
W*KMSLYICSNPQNI*HQELDLM*TMDFG***C
ANVSS*IIANVPLRWEMLLMGEAMHVWEQSVSGS
SPQPFWQQGPVLWKTIFPQIVGGMGV*SGDETV
KLFHLRSTSIS*ILIRNMQPRSLACTVKNRVRT
PMRI*CHH*SDGRRGSVGNAGSPTAWSSF*QATN
WYWSMARGLGTSGIPLHLLLSFSVNLQPL*NKV
YF*KGKEKVTNYDPKCVK*KF*YEKQSH*NAQR
AKKLLAIGFQIYFIYISMIREARTQHFGVVCICV
CVCVCVCVSVI*GVY*MA**PESCRNLQMLPNS
DSFLKQHCGKQI***QYFIKRMYSKNIYVMLK*
QN*N*F*KIRVNSSFYQLKM*GLLSIGLQIYLPD
LKSCNS*TII*AI*IISDYTHKV*LH*YLISHY
LKFTKLVGHRGSCL*S*HFGGQ*GRIA*SQEFN
SCLGNKARPCLYNTKIN*CMAALL*SQLL*RLRQ
EECLSPGVQYFSGLRLCHCAHSSLGSRAGLHL*
K*INK*NLQNFKNQMKYVRFVPTTFKLGT*RIK
HFITNEALHAQTGT**VVDRC*QFVLLLFSGVQL
TNPTVNDITGMQPTRSRIWKLLLDKQLHFFSNN
SREREKRSYTF*KAEEIYEPNLYEAIRKTDTDC
IKELSNFSVVMRLLLCFLKSHYTLDFHNKIIMNE
Frame 2
RFQFNVLPLFIEQFLKSTLSRGCITRGLPRLLPGLLIQTLQLVTEMSP*GIFLIGQNLTWQGLVWV
SSDFLVLRSSQLLYSSEHQLLRLPWEEDDPKQS
SAAGGPRSDHRDEPLWR*RHCG*PRCLLWCKLVP
VLRSLWPVHP*I*WR*AVLRGPGEEGDCLAVA*
VQQIWRF*PAGCTEKHGCGKTQLEHHD*TLQLY
RCYQ*GS*GHSVFQVSRDTGSAQHPHLSCGQHLS
SCGQHHMAEQWAVSHRRCF*DQLPLQE*SFLLQ
DQLPHLPPFC**DL*LQGGALGPGPASSETLGV
TYKDMPGVSHPAT*FLSNLHLKSPRKQ*IPFMRS
MSNFSIFHPGLTETMANNWGTLMFQSNLTSFPR
SFFMSSNTEATKYSIA**YVDFLADINISCFLV
FPPLALPPTPQFRQQ*N*WIPSALGPELL*QKF*
NQKISLY*FQCGF*KYDREIS*DKGNLNLKNIK
SKNVFSKL*IYEE**Q*KPSSSLVTLR*KFFRQ
EKKWKSEKH*NKTIMWVSEKEQILIPHIGLVTMG
KGTGVEATDISRSPE*RRSLPHLLNEAVLDNHV
AFPVPLWHEGDSIVNMATGCFLETCQYLQKSPA
LSPNPS*LRKA*AYERNPRKNNTVETM*QQQWVL
CPPLDSPSPRRNSHRGNGSAATSWL*TAMKSMR
IFLTMLPASVSLNFNWRKNTKH*CNN*YSIVWC
KEP*IQIQDSVL*S*YFKLYKCVKSLTFLALLFS
STM*E**YFPCRVIDKI*IILIFSQCLTHST*T
HKKTLWLYL*LIYLKEWIILYEKHFCFSQPLND
LGDSNVELWVNFFSYDN*RIFFFFQNERG*DWIG
KRTLSTRSCNI*LWFSTL*EGIYSLLMAHKCLF
KVYHIQQIFMPVYFGEEVKIVQGENNLKHRLGI
SKHRKSEPVMIMKMSITEHR*FSGQ*NYSV*YHN
GEKCHYTFAQIHRIYNTKS*T*CKLWTLGDNDV
PM*VHKL*QMYHSDGRCC*WGRLCMCGSRVYLA
AVPSLFGSRDQFYGRQFFHR*LGEWGYDLGMKL*
NYSTSDQQALVRFS*GTCNLDLLHAQLKIGFAL
L*ESNATTDLTEGGAQLVMLAHPLRGPVSNRPP
TGTGPWPGGWGPLGYLCTFCSVFL*TYNRFKIRS
IFKKEKRR*PIMILNV*NKNFSMKNSHIKMHNV
LRSF*Q*GFKYISYTFQ*FVRQEPSILELCAFV
CVCVCVCVCL*YKGYTEWHNDQSHAEIYKCCPTQ
TPSSSSTVESKFNDSSILLKGCIQKIFT*C*NS
RIKTNSKK*E*IVLFIS*KCRAYYQ*GYRSTCL
I*NHAILKQLFELFELFQITHIKCDFINT*YHTI
*NLQN*WVTEARACDLSTLEANRGGLLEARSST
AAWATKQDLVSTTQK*INAWRHSCSPSYSRG*G
KRSA*AQEFNTSVG*DCATVLTPA*AAEQDSISK
SK*INKTYKILKIK*NMSGLYLPHLN*GLEELN
ILLQMKHFMHRLAHSK*SIGVNNLCYCYFLESN
*QIPQ*MTSQGCNQQDPEYGNFY*INNSISSATI
QERERREAIHFKRLKKYMNQICMRQSEKLTPTV
LRNYLILVW**DCCYVF*KVIIL*IFIIK*L*M
K
Sequence HLA-DQA1.aAug05
AAGAGCAAAATAATATGAAAAGTATTTAACAAGGAATATAAAGAATCATCAAAGAAAATGTTATAAAACTTCTTTGAGACCACAAGAGATGATAGAGATGGGGTTTCACTGTGTTAGCGAGGATGGTCTTGATCTCCTGACCTCGTGATCCGCCTGCTTCTGCCTCCCAAAGTGCTGGGATTACAGTCGTGAGCCACCGC
GCCTGGCCTCCTCATATTATTTTTTATTGTGCAGTTTATTCAAGAGAGCTTTCCAGCACCTGTGATAAGCCTCAGCCCGCCTGCCGGATAATGAGCCCAC
ATGGATTGAGAGAGGCCTCCTATCCCTGCCAAACCTATTGATGCTACAGAGATGTGAGGGAGCCCACCTGAGAAAAGCTGAACCTGCCCAGTACATAAAA
ACCACTCAGCTCAGAACACCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGAT
GAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCTGGCCAGTACACCCATGAA
TTTGATGGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTG
CACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTT
TTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATTTGTCTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGG
CAGTCAGTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTCCCTTCTGCTGATG
AGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGAC
TGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACAC
CAAGGGCCATTGTGAATCCCATCCTGGAAGGGAAGTGTTACCTACAAAGACATGCCTGGGGTAAGCCACCCGGCTACCTAATTCCTCAGTAACCTCCATC
TAAAATCTCCAAGGAAGCAATAAATTCCTTTTATGAGATCTATGTCAAATTTTTCCATCTTTCATCCAGGGCTGACTGAAACTATGGCTAATAATTGGGG
TACTCTTATGTTTCAATCCAATTTAACCTCATTTCCCAGATCATTTTTCATGTCCAGTAACACAGAAGCCACCAAGTACAGTATAGCCTGATAATATGTT
GATTTCTTAGCTGACATTAATATTTCTTGCTTCCTTGTGTTCCCACCCTTGGCACTGCCACCCACCCCTCAATTCAGGCAACAATGAAATTAATGGATAC
CGTCTGCCCTTGGCCCAGAATTGTTATAGCAAAAATTTTAGAACCAAAAAATAAGTCTGTACTAATTTCAATGTGGCTTTTAAAAGTATGACAGAGAAAT
AAGTTAGGATAAAGGAAATTTGAATCTCAAAAATATCAAAAGTAAAAATGTATTCTCAAAACTTTAAATTTATGAAGAATGATGACAGTAGAAGCCTTCC
TCTTCCCTTGTCACCTTGAGATAAAAATTCTTTAGGCAGGAAAAGAAATGGAAGTCAGAAAAACATTAGAATAAGACAATAATGTGGGTGTCTGAAAAGG
AACAAATACTTATTCCTCACATAGGGTTAGTGACAATGGGAAAAGGGACGGGAGTAGAAGCCACAGACATATCTAGGAGCCCTGAATAGAGGCGCAGTCT
GCCTCACCTCCTGAATGAAGCTGTACTAGATAACCATGTAGCTTTCCCTGTGCCACTCTGGCATGAAGGAGACAGTATAGTGAATATGGCTACAGGATGT
TTCTTGGAAACATGCCAATATCTTCAGAAATCCCCAGCCCTTTCCCCTAACCCCTCCTGACTAAGGAAAGCATGAGCTTATGAGAGAAACCCTAGGAAGA
ACAACACAGTTGAGACAATGTAGCAGCAGCAGTGGGTGCTGTGTCCTCCACTGGATTCGCCATCTCCGAGGAGAAACTCTCACAGAGGAAATGGGTCAGC
AGCGACCTCATGGCTCTAAACAGCTATGAAATCCATGAGGATATTTCTAACCATGCTACCTGCATCAGTGAGTTTAAATTTTAATTGGAGAAAAAATACA
AAACATTAATGCAATAATTGATACAGTATAGTTTGGTGCAAAGAACCCTAAATCCAAATCCAGGACTCAGTACTTTGAAGCTAGTATTTTAAACTTTATA
AATGTGTGAAGTCTCTAACATTTCTGGCCTTACTTTTCTCATCCACAATGTAGGAGTAATAATACTTTCCTTGCAGAGTTATTGATAAAATTTGAATAAT
TTTGATATTTAGTCAATGCCTTACACATAGTACATGAACACATAAGAAAACATTGTGGTTATATTTATAATTAATTTATTTAAAAGAATGGATCATATTA
TATGAAAAGCACTTTTGTTTTTCTCAGCCTCTTAATGATTTAGGAGATTCAAATGTAGAGCTATGGGTGAATTTCTTTTCATATGATAATTGAAGGATAT
TTTTCTTTTTCCAGAATGAGAGAGGCTGAGATTGGATTGGTAAGAGAACTCTTAGCACTAGAAGTTGTAATATTTGACTTTGGTTTTCAACTCTCTAAGA
GGGAATATATTCCCTCCTTATGGCCCATAAGTGTTTATTCAAGGTATATCATATACAACAGATCTTTATGCCTGTTTACTTTGGGGAAGAAGTGAAGATA
GTTCAAGGAGAAAATAATTTAAAACACAGACTGGGAATCAGTAAGCACAGGAAATCTGAACCAGTGATGATCATGAAAATGTCCATCACAGAGCACAGAT
GATTTTCAGGGCAATAAAACTATTCTGTTTGATACCACAATGGTGAAAAATGTCATTATACATTTGCTCAAATCCACAGAATATATAACACCAAGAGTTA
GACTTAATGTAAACTATGGACTTTGGGTGATAATGATGTGCCAATGTAAGTTCATAAATTATAGCAAATGTACCACTCAGATGGGAGATGTTGCTAATGG
GGGAGGCTATGCATGTGTGGGAGCAGAGTGTATCTGGCAGCAGTCCCCAGCCTTTTTGGCAGCAGGGACCAGTTTTATGGAAGACAATTTTTCCACAGAT
AGTTGGGGGAATGGGGGTATGATCTGGGGATGAAACTGTGAAACTATTCCACCTCAGATCAACAAGCATTAGTTAGATTCTCATAAGGAACATGCAACCT
AGATCTCTTGCATGCACAGTTAAAAATAGGGTTCGCACTCCTATGAGAATCTAATGCCACCACTGATCTGACGGAAGGCGGGGCTCAGTTGGTAATGCTG
GCTCACCCACTGCGTGGTCCAGTTTCTAACAGGCCACCAACTGGTACTGGTCCATGGCCCGGGGGTTGGGGACCTCTGGGATACCTCTGCACCTTCTGCT
CAGTTTTTCTGTGAACCTACAACCGCTTTAAAATAAGGTCTATTTTTAAAAAGGAAAAGAGAAGGTAACCAATTATGATCCTAAATGTGTAAAATAAAAA
TTTTAGTATGAAAAACAGTCACATTAAAATGCACAACGTGCTAAGAAGCTTTTAGCAATAGGGTTTCAAATATATTTCATATACATTTCAATGATTCGTG
AGGCAAGAACCCAGCATTTTGGAGTTGTGTGCATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAATATAAGGGGTATACTGAATGGCATAATG
ACCAGAGTCATGCAGAAATCTACAAATGCTGCCCAACTCAGACTCCTTCCTCAAGCAGCACTGTGGAAAGCAAATTTAATGATAGCAGTATTTTATTAAA
AGGATGTATTCAAAAAATATTTACGTAATGTTAAAATAGCAGAATTAAAACTAATTCTAAAAAATAAGAGTAAATAGTTCTTTTTATCAGCTAAAAATGT
AGGGCCTACTATCAATAGGGTTACAGATCTACCTGCCTGATTTAAAATCATGCAATTCTTAAACAATTATTTGAGCTATTTGAATTATTTCAGATTACAC
ACATAAAGTGTGACTTCATTAATACTTAATATCACACTATTTAAAATTTACAAAATTAGTGGGTCACAGAGGCTCGTGCTTGTGATCTTAGCACTTTGGA
GGCCAATAGGGGAGGATTGCTTGAAGCCAGGAGTTCAACAGCTGCCTGGGCAACAAAGCAAGACCTTGTCTCTACAACACAAAAATAAATTAATGCATGG
CGGCACTCCTGTAGTCCCAGCTACTCTAGAGGCTGAGGCAAGAGGAGTGCTTGAGCCCAGGAGTTCAATACTTCAGTGGGCTAAGATTGTGCCACTGTGC
TCACTCCAGCCTAGGCAGCAGAGCAGGACTCCATCTCTAAAAGTAAATAAATAAATAAAACTTACAAAATTTTAAAAATCAAATGAAATATGTCAGGTTT
GTACCTACCACATTTAAACTAGGGACTTGAAGAATTAAACATTTTATTACAAATGAAGCACTTCATGCACAGACTGGCACATAGTAAGTAGTCGATAGGT
GTTAACAATTTGTGTTATTGTTATTTTCTGGAGTCCAACTAACAAATCCCACAGTGAATGACATCACAGGGATGCAACCAACAAGATCCAGAATATGGAA
ACTTCTACTAGATAAACAACTCCATTTCTTCAGCAACAATTCAAGAGAGAGAGAGAAGAGAAGCTATACATTTTAAAAGGCTGAAGAAATATATGAACCA
AATTTGTATGAGGCAATCAGAAAAACTGACACCGACTGTATTAAGGAATTATCTAATTTTAGTGTGGTAATGAGATTGCTGTTATGTTTTCTAAAAAGTC
ATTATACTTTAGATTTTCATAATAAAATAATTATGAATGAAA
- HLA-DQA1.F3 (forward primer)
- HLA-DQA1.R3 (reverse primer)
- Single exon skipping
Frame 0
KSKII*KVFNKEYKESSKKML*NFFETTRDDRDGVSLC*RGWS*SPDLVIRLLLPPKVLGLQS*ATA
PGLLILFFIVQFIQESFPAPVISLSPPAG**AH
MD*ERPPIPAKPIDATEM*GSPPEKS*TCPVHK
NHSAQNTNC*GCLGKRMILNKALLLGALALTTVM
SPCGGEDIVADHVASCGVNLYQFYGPSGQYTHE
FDGDEQFYVDLERKETAWRWPEFSKFGGFDPQG
ALRNMAVAKHNLNIMIKRYNSTAATNEVPEVTVF
SKSPVTLGQPNTLICLVDNIFPPVVNITWLSNG
QSVTEGVSETSFLSKSDHSFFKISYLTFLPSAD
EIYDCKVEHWGLDQPLLKHWEPEIPAPMSELTET
VVCALGLSVGLMGIVVGTVFIIQGLRSVGASRH
QGPL*IPSWKGSVTYKDMPGVSHPAT*FLSNLH
LKSPRKQ*IPFMRSMSNFSIFHPGLTETMANNWG
TLMFQSNLTSFPRSFFMSSNTEATKYSIA**YV
DFLADINISCFLVFPPLALPPTPQFRQQ*N*WI
PSALGPELL*QKF*NQKISLY*FQCGF*KYDREI
S*DKGNLNLKNIKSKNVFSKL*IYEE**Q*KPS
SSLVTLR*KFFRQEKKWKSEKH*NKTIMWVSEK
EQILIPHIGLVTMGKGTGVEATDISRSPE*RRSL
PHLLNEAVLDNHVAFPVPLWHEGDSIVNMATGC
FLETCQYLQKSPALSPNPS*LRKA*AYERNPRK
NNTVETM*QQQWVLCPPLDSPSPRRNSHRGNGSA
ATSWL*TAMKSMRIFLTMLPASVSLNFNWRKNT
KH*CNN*YSIVWCKEP*IQIQDSVL*S*YFKLY
KCVKSLTFLALLFSSTM*E**YFPCRVIDKI*II
LIFSQCLTHST*THKKTLWLYL*LIYLKEWIIL
YEKHFCFSQPLNDLGDSNVELWVNFFSYDN*RI
FFFFQNERG*DWIGKRTLSTRSCNI*LWFSTL*E
GIYSLLMAHKCLFKVYHIQQIFMPVYFGEEVKI
VQGENNLKHRLGISKHRKSEPVMIMKMSITEHR
*FSGQ*NYSV*YHNGEKCHYTFAQIHRIYNTKS*
T*CKLWTLGDNDVPM*VHKL*QMYHSDGRCC*W
GRLCMCGSRVYLAAVPSLFGSRDQFYGRQFFHR
*LGEWGYDLGMKL*NYSTSDQQALVRFS*GTCNL
DLLHAQLKIGFALL*ESNATTDLTEGGAQLVML
AHPLRGPVSNRPPTGTGPWPGGWGPLGYLCTFC
SVFL*TYNRFKIRSIFKKEKRR*PIMILNV*NKN
FSMKNSHIKMHNVLRSF*Q*GFKYISYTFQ*FV
RQEPSILELCAFVCVCVCVCVCL*YKGYTEWHN
DQSHAEIYKCCPTQTPSSSSTVESKFNDSSILLK
GCIQKIFT*C*NSRIKTNSKK*E*IVLFIS*KC
RAYYQ*GYRSTCLI*NHAILKQLFELFELFQIT
HIKCDFINT*YHTI*NLQN*WVTEARACDLSTLE
ANRGGLLEARSSTAAWATKQDLVSTTQK*INAW
RHSCSPSYSRG*GKRSA*AQEFNTSVG*DCATV
LTPA*AAEQDSISKSK*INKTYKILKIK*NMSGL
YLPHLN*GLEELNILLQMKHFMHRLAHSK*SIG
VNNLCYCYFLESN*QIPQ*MTSQGCNQQDPEYG
NFY*INNSISSATIQERERREAIHFKRLKKYMNQ
ICMRQSEKLTPTVLRNYLILVW**DCCYVF*KV
IIL*IFIIK*L*MK
Frame 1
RAK*YEKYLTRNIKNHQRKCYKTSLRPQEMIEMGFHCVSEDGLDLLTS*SACFCLPKCWDYSREPP
RLASSYYFLLCSLFKRAFQHL**ASARLPDNEPT
WIERGLLSLPNLLMLQRCEGAHLRKAEPAQYIK
TTQLRTPTAEAALGRG*S*TKLCCWGPSL*PP*
*APVEVKTLWLTTLPLVV*TCTSFTVPLASTPMN
LMEMSSSTWTWRGRRLPGGGLSSANLEVLTRRV
H*ETWLWQNTT*TS*LNATTLPLLPMRFLRSQC
FPSLP*HWVSPTPSFVLWTTSFLLWSTSHG*AMG
SQSQKVFLRPASSPRVIIPSSRSVTSPSSLLLM
RFMTARWSTGAWTSLF*NTGSLRFQPLCQSSQR
LWSVPWGCLWASWALWWALSSSSKACVQLVLPDT
KGHCESHPGREVLPTKTCLG*ATRLPNSSVTSI
*NLQGSNKFLL*DLCQIFPSFIQG*LKLWLIIG
VLLCFNPI*PHFPDHFSCPVTQKPPSTV*PDNML
IS*LTLIFLASLCSHPWHCHPPLNSGNNEINGY
RLPLAQNCYSKNFRTKK*VCTNFNVAFKSMTEK
*VRIKEI*ISKISKVKMYSQNFKFMKNDDSRSLP
LPLSP*DKNSLGRKRNGSQKNIRIRQ*CGCLKR
NKYLFLT*G**QWEKGRE*KPQTYLGALNRGAV
CLTS*MKLY*ITM*LSLCHSGMKETV**IWLQDV
SWKHANIFRNPQPFPLTPPD*GKHELMRETLGR
TTQLRQCSSSSGCCVLHWIRHLRGETLTEEMGQ
QRPHGSKQL*NP*GYF*PCYLHQ*V*ILIGEKIQ
NINAIIDTV*FGAKNPKSKSRTQYFEASILNFI
NV*SL*HFWPYFSHPQCRSNNTFLAELLIKFE*
F*YLVNALHIVHEHIRKHCGYIYN*FI*KNGSYY
MKSTFVFLSLLMI*EIQM*SYG*ISFHMIIEGY
FSFSRMREAEIGLVRELLALEVVIFDFGFQLSK
REYIPSLWPISVYSRYIIYNRSLCLFTLGKK*R*
FKEKII*NTDWESVSTGNLNQ**S*KCPSQSTD
DFQGNKTILFDTTMVKNVIIHLLKSTEYITPRV
RLNVNYGLWVIMMCQCKFINYSKCTTQMGDVANG
GGYACVGAECIWQQSPAFLAAGTSFMEDNFSTD
SWGNGGMIWG*NCETIPPQINKH*LDSHKEHAT
*ISCMHS*K*GSHSYENLMPPLI*RKAGLSW*CW
LTHCVVQFLTGHQLVLVHGPGVGDLWDTSAPSA
QFFCEPTTALK*GLFLKRKREGNQL*S*MCKIK
ILV*KTVTLKCTTC*EAFSNRVSNIFHIHFNDS*
GKNPAFWSCVHLCVCVCVCVCVCNIRGILNGIM
TRVMQKSTNAAQLRLLPQAALWKANLMIAVFY*
KDVFKKYLRNVKIAELKLILKNKSK*FFLSAKNV
GPTINRVTDLPA*FKIMQFLNNYLSYLNYFRLH
T*SVTSLILNITLFKIYKISGSQRLVLVILALW
RPIGEDCLKPGVQQLPGQQSKTLSLQHKNKLMHG
GTPVVPATLEAEARGVLEPRSSILQWAKIVPLC
SLQPRQQSRTPSLKVNK*IKLTKF*KSNEICQV
CTYHI*TRDLKN*TFYYK*STSCTDWHIVSSR*V
LTICVIVIFWSPTNKSHSE*HHRDATNKIQNME
TSTR*TTPFLQQQFKREREEKLYILKG*RNI*T
KFV*GNQKN*HRLY*GII*F*CGNEIAVMFSKKS
LYFRFS**NNYE*
Frame 2
EQNNMKSI*QGI*RIIKENVIKLL*DHKR**RWGFTVLARMVLIS*PRDPPASASQSAGITVVSHR
AWPPHIIFYCAVYSRELSSTCDKPQPACRIMSP
HGLREASYPCQTY*CYRDVREPT*EKLNLPST*K
PLSSEHQLLRLPWEEDDPKQSSAAGGPRSDHRD
EPLWR*RHCG*PRCLLWCKLVPVLRSLWPVHP*
I*WR*AVLRGPGEEGDCLAVA*VQQIWRF*PAGC
TEKHGCGKTQLEHHD*TLQLYRCYQ*GS*GHSV
FQVSRDTGSAQHPHLSCGQHLSSCGQHHMAEQW
AVSHRRCF*DQLPLQE*SFLLQDQLPHLPPFC**
DL*LQGGALGPGPASSETLGA*DSSPYVRAHRD
CGLCPGVVCGPHGHCGGHCLHHPRPAFSWCFQT
PRAIVNPILEGKCYLQRHAWGKPPGYLIPQ*PPS
KISKEAINSFYEIYVKFFHLSSRAD*NYG**LG
YSYVSIQFNLISQIIFHVQ*HRSHQVQYSLIIC
*FLS*H*YFLLPCVPTLGTATHPSIQATMKLMDT
VCPWPRIVIAKILEPKNKSVLISMWLLKV*QRN
KLG*RKFESQKYQK*KCILKTLNL*RMMTVEAF
LFPCHLEIKIL*AGKEMEVRKTLE*DNNVGV*KG
TNTYSSHRVSDNGKRDGSRSHRHI*EP*IEAQS
ASPPE*SCTR*PCSFPCATLA*RRQYSEYGYRM
FLGNMPISSEIPSPFP*PLLTKESMSL*EKP*EE
QHS*DNVAAAVGAVSSTGFAISEEKLSQRKWVS
SDLMALNSYEIHEDISNHATCISEFKF*LEKKY
KTLMQ*LIQYSLVQRTLNPNPGLSTLKLVF*TL*
MCEVSNISGLTFLIHNVGVIILSLQSY**NLNN
FDI*SMPYT*YMNT*ENIVVIFIINLFKRMDHI
I*KALLFFSAS**FRRFKCRAMGEFLFI**LKDI
FLFPE*ERLRLDW*ENS*H*KL*YLTLVFNSLR
GNIFPPYGP*VFIQGISYTTDLYACLLWGRSED
SSRRK*FKTQTGNQ*AQEI*TSDDHENVHHRAQM
IFRAIKLFCLIPQW*KMSLYICSNPQNI*HQEL
DLM*TMDFG***CANVSS*IIANVPLRWEMLLM
GEAMHVWEQSVSGSSPQPFWQQGPVLWKTIFPQI
VGGMGV*SGDETVKLFHLRSTSIS*ILIRNMQP
RSLACTVKNRVRTPMRI*CHH*SDGRRGSVGNA
GSPTAWSSF*QATNWYWSMARGLGTSGIPLHLLL
SFSVNLQPL*NKVYF*KGKEKVTNYDPKCVK*K
F*YEKQSH*NAQRAKKLLAIGFQIYFIYISMIR
EARTQHFGVVCICVCVCVCVCVSVI*GVY*MA**
PESCRNLQMLPNSDSFLKQHCGKQI***QYFIK
RMYSKNIYVMLK*QN*N*F*KIRVNSSFYQLKM
*GLLSIGLQIYLPDLKSCNS*TII*AI*IISDYT
HKV*LH*YLISHYLKFTKLVGHRGSCL*S*HFG
GQ*GRIA*SQEFNSCLGNKARPCLYNTKIN*CM
AALL*SQLL*RLRQEECLSPGVQYFSGLRLCHCA
HSSLGSRAGLHL*K*INK*NLQNFKNQMKYVRF
VPTTFKLGT*RIKHFITNEALHAQTGT**VVDR
C*QFVLLLFSGVQLTNPTVNDITGMQPTRSRIWK
LLLDKQLHFFSNNSREREKRSYTF*KAEEIYEP
NLYEAIRKTDTDCIKELSNFSVVMRLLLCFLKS
HYTLDFHNKIIMNE
Copyright ©2008,2017, all rights reserved. 