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Sequences

Sequence IL1RN.eAug05

TGTACTCTCTGAGGTGCTCTGGAAGGAGGGGCAGCTCCACCCTGGGAGGGACTGTGGCCCAGGTACTGCCCGGGTGCTACTTTATGGGCAGCAGCTCAGT
TGAGTTAGAGTCTGGAAGACCTCAGAAGACCTCCTGTCCTATGAGGCCCTCCCCATGGCTTTAGGGGGATTATAAAACTAATCATCAAAGCCAAGAAGGC
AAGAGCAAGCATGTACCGCTGAAAACACAAGATAACTGCATAAGTAATGACTTTCAGTGCAGATTCATAGCTAACCCATAAACTGCTGGGGCAAAAATCA
TCTTGGAAGGCTCTGAACCTCAGAAAGGATTCACAAGACGATCTGCCGACCCTCTGGGAGAAAATCCAGCAAGATGCAAGCCTTCAGAATCTGGGATGTT
AACCAGAAGACCTTCTATCTGAGGAACAACCAACTAGTTGCTGGATACTTGCAAGGACCAAATGTCAATTTAGAAGAAAAGATAGATGTGGTACCCATTG
AGCCTCATGCTCTGTTCTTGGGAATCCATGGAGGGAAGATGTGCCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGTTAACAT
CACTGACCTGAGCGAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGCTCAGACAGTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGT
TGGTTCCTCTGCACAGCGATGGAAGCTGACCAGCCCGTCAGCCTCACCAATATGCCTGACGAAGGCGTCATGGTCACCAAATTCTACTTCCAGGAGGACG
AGTAGTACTGCCCAGGCCTGCCTGTTCCCATTCTTGCATGGCAAGGACTGCAGGGACTGCCAGTCCCCCTGCCCCAGGGCTCCCGGCTATGGGGGCACTG
AGGACCAGCCATTGAGGGGTGGACCCTCAGAAGGCGTCACAACAACCTGGTCACAGGACTCTGCCTCCTCTTCAACTGACCAGCCTCCATGCTGCCTCCA
GAATGGTCTTTCTAATGTGTGAATCAGAGCACAGCAGCCCCTGCACAAAGCCCTTCCATGTCGCCTCTGCATTCAGGATCAAACCCCGACCACCTGCCCA
ACCTGCTCTCCTCTTGCCACTGCCTCTTCCTCCCTCATTCCACCTTCCCATGCCCTGGATCCATCAGGCCACTTGATGACCCCCAACCAAGTGGCTCCCA
CACCCTGTTTTACAAAAAAGAAAAGACCAGTCCATGAGGGAGGTTTTTAAGGGTTTGTGGAAAATGAAAATTAGGATTTCATGATTTTTTTTTTTCAGTC
CCCGTGAAGGAGAGCCCTTCATTTGGAGATTATGTTCTTTCGGGGAGAGGCTGAGGACTTAAAATATTCCTGCATTTGTGAAATGATGGTGAAAGTAAGT
GGTAGCTTTTCCCTTCTTTTTCTTCTTTTTTTGTGATGTCCCAACTTGTAAAAATTAAAAGTTATGGTACTATGTTAGCCCCATAATTTTTTTTTTCCTT
TTAAAACACTTCCATAATCTGGACTCCTCTGTCCAGGCACTGCTGCCCAGCCTCCAAGCTCCATCTCCACTCCAGATTTTTTACAGCTGCCTGCAGTACT
TTACCTCCTATCAGAAGTTTCTCAGCTCCCAAGGCTCTGAGCAAATGTGGCTCCTGGGGGTTCTTTCTTCCTCTGCTGAAGGAATAAATTGCTCCTTGAC
ATTGTAGAGCTTCTGGCACTTGGAGACTTGTATGAAAGATGGCTGTGCCTCTGCCTGTCTCCCCCACCGGGCTGGGAGCTCTGCAGAGCAGGAAACATGA
CTCGTATATGTCTCAGGTCCCTGCAGGGCCAAGCACCTAGCCTCGCTCTTGGCAGGTACTCAGCGAATGAATGCTGTATATGTTGGGTGCAAAGTTCCCT
ACTTCCTGTGACTTCAGCTCTGTTTTACAATAAAATCTTGAAAATGCCTATA
  • IL1RN.F5 (forward primer)
  • IL1RN.R9 (reverse primer)
  • Single exon skipping

Frame 0

CTL*GALEGGAAPPWEGLWPRYCPGATLWAAAQ
LS*SLEDLRRPPVL*GPPHGFRGIIKLIIKAKKA
RASMYR*KHKITA*VMTFSADS*LTHKLLGQKS
SWKALNLRKDSQDDLPTLWEKIQQDASLQNLGC
*PEDLLSEEQPTSCWILARTKCQFRRKDRCGTH*
ASCSVLGNPWREDVPVLCQVW**DQTPAGGS*H
H*PEREQKAGQALRLHPLRQWPHHQF*VCRLPR
LVPLHSDGS*PARQPHQYA*RRRHGHQILLPGGR
VVLPRPACSHSCMARTAGTASPPAPGLPAMGAL
RTSH*GVDPQKASQQPGHRTLPPLQLTSLHAAS
RMVFLMCESEHSSPCTKPFHVASAFRIKPRPPAQ
PALLLPLPLPPSFHLPMPWIHQAT**PPTKWLP
HPVLQKRKDQSMREVFKGLWKMKIRIS*FFFFS
PREGEPFIWRLCSFGERLRT*NIPAFVK*W*K*V
VAFPFFFFFFCDVPTCKN*KLWYYVSPIIFFFL
LKHFHNLDSSVQALLPSLQAPSPLQIFYSCLQY
FTSYQKFLSSQGSEQMWLLGVLSSSAEGINCSLT
L*SFWHLETCMKDGCASACLPHRAGSSAEQET*
LVYVSGPCRAKHLASLLAGTQRMNAVYVGCKVP
YFL*LQLCFTIKS*KCL

Frame 1

VLSEVLWKEGQLHPGRDCGPGTARVLLYGQQLS
*VRVWKTSEDLLSYEALPMALGGL*N*SSKPRR
QEQACTAENTR*LHK**LSVQIHS*PINCWGKNH
LGRL*TSERIHKTICRPSGRKSSKMQAFRIWDV
NQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPI
EPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNI
TDLSENRKQDKRFAFIRSDSGPTTSFESAACPG
WFLCTAMEADQPVSLTNMPDEGVMVTKFYFQED
E*YCPGLPVPILAWQGLQGLPVPLPQGSRLWGH*
GPAIEGWTLRRRHNNLVTGLCLLFN*PASMLPP
EWSF*CVNQSTAAPAQSPSMSPLHSGSNPDHLP
NLLSSCHCLFLPHSTFPCPGSIRPLDDPQPSGSH
TLFYKKEKTSP*GRFLRVCGK*KLGFHDFFFSV
PVKESPSFGDYVLSGRG*GLKIFLHL*NDGESK
W*LFPSFSSFFVMSQLVKIKSYGTMLAP*FFFSF
*NTSIIWTPLSRHCCPASKLHLHSRFFTAACST
LPPIRSFSAPKALSKCGSWGFFLPLLKE*IAP*
HCRASGTWRLV*KMAVPLPVSPTGLGALQSRKHD
SYMSQVPAGPST*PRSWQVLSE*MLYMLGAKFP
TSCDFSSVLQ*NLENAY

Frame 2

YSLRCSGRRGSSTLGGTVAQVLPGCYFMGSSSV
ELESGRPQKTSCPMRPSPWL*GDYKTNHQSQEG
KSKHVPLKTQDNCISNDFQCRFIANP*TAGAKI
ILEGSEPQKGFTRRSADPLGENPARCKPSESGML
TRRPSI*GTTN*LLDTCKDQMSI*KKR*MWYPL
SLMLCSWESMEGRCACPVSSLVMRPDSSWRQLT
SLT*ARTESRTSASPSSAQTVAPPPVLSLPPAPV
GSSAQRWKLTSPSASPICLTKASWSPNSTSRRT
SSTAQACLFPFLHGKDCRDCQSPCPRAPGYGGT
EDQPLRGGPSEGVTTTWSQDSASSSTDQPPCCLQ
NGLSNV*IRAQQPLHKALPCRLCIQDQTPTTCP
TCSPLATASSSLIPPSHALDPSGHLMTPNQVAP
TPCFTKKKRPVHEGGF*GFVENEN*DFMIFFFQS
P*RRALHLEIMFFRGEAEDLKYSCICEMMVKVS
GSFSLLFLLFL*CPNL*KLKVMVLC*PHNFFFP
FKTLP*SGLLCPGTAAQPPSSISTPDFLQLPAVL
YLLSEVSQLPRL*ANVAPGGSFFLC*RNKLLLD
IVELLALGDLYERWLCLCLSPPPGWELCRAGNM
TRICLRSLQGQAPSLALGRYSANECCICWVQSSL
LPVTSALFYNKILKMPI

Sequence IL1RN.fAug05

TGTACTCTCTGAGGTGCTCTGGAAGGAGGGGCAGCTCCACCCTGGGAGGGACTGTGGCCCAGGTACTGCCCGGGTGCTACTTTATGGGCAGCAGCTCAGT
TGAGTTAGAGTCTGGAAGACCTCAGAAGACCTCCTGTCCTATGAGGCCCTCCCCATGGCTTTAGCTGACTTGTATGAAGAAGGAGGTGGAGGAGGAGGAG
AAGGTGAAGACAATGCTGACTCAAAGGGGGGATTATAAAACTAATCATCAAAGCCAAGAAGGCAAGAGCAAGCATGTACCGCTGAAAACACAAGATAACT
GCATAAGTAATGACTTTCAGTGCAGATTCATAGCTAACCCATAAACTGCTGGGGCAAAAATCATCTTGGAAGGCTCTGAACCTCAGAAAGGATTCACAAG
ACGATCTGCCGACCCTCTGGGAGAAAATCCAGCAAGATGCAAGCCTTCAGAATCTGGGATGTTAACCAGAAGACCTTCTATCTGAGGAACAACCAACTAG
TTGCTGGATACTTGCAAGGACCAAATGTCAATTTAGAAGAAAAGATAGATGTGGTACCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGAGGGAA
GATGTGCCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGTTAACATCACTGACCTGAGCGAGAACAGAAAGCAGGACAAGCGC
TTCGCCTTCATCCGCTCAGACAGTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGCACAGCGATGGAAGCTGACCAGCCCG
TCAGCCTCACCAATATGCCTGACGAAGGCGTCATGGTCACCAAATTCTACTTCCAGGAGGACGAGTAGTACTGCCCAGGCCTGCCTGTTCCCATTCTTGC
ATGGCAAGGACTGCAGGGACTGCCAGTCCCCCTGCCCCAGGGCTCCCGGCTATGGGGGCACTGAGGACCAGCCATTGAGGGGTGGACCCTCAGAAGGCGT
CACAACAACCTGGTCACAGGACTCTGCCTCCTCTTCAACTGACCAGCCTCCATGCTGCCTCCAGAATGGTCTTTCTAATGTGTGAATCAGAGCACAGCAG
CCCCTGCACAAAGCCCTTCCATGTCGCCTCTGCATTCAGGATCAAACCCCGACCACCTGCCCAACCTGCTCTCCTCTTGCCACTGCCTCTTCCTCCCTCA
TTCCACCTTCCCATGCCCTGGATCCATCAGGCCACTTGATGACCCCCAACCAAGTGGCTCCCACACCCTGTTTTACAAAAAAGAAAAGACCAGTCCATGA
GGGAGGTTTTTAAGGGTTTGTGGAAAATGAAAATTAGGATTTCATGATTTTTTTTTTTCAGTCCCCGTGAAGGAGAGCCCTTCATTTGGAGATTATGTTC
TTTCGGGGAGAGGCTGAGGACTTAAAATATTCCTGCATTTGTGAAATGATGGTGAAAGTAAGTGGTAGCTTTTCCCTTCTTTTTCTTCTTTTTTTGTGAT
GTCCCAACTTGTAAAAATTAAAAGTTATGGTACTATGTTAGCCCCATAATTTTTTTTTTCCTTTTAAAACACTTCCATAATCTGGACTCCTCTGTCCAGG
CACTGCTGCCCAGCCTCCAAGCTCCATCTCCACTCCAGATTTTTTACAGCTGCCTGCAGTACTTTACCTCCTATCAGAAGTTTCTCAGCTCCCAAGGCTC
TGAGCAAATGTGGCTCCTGGGGGTTCTTTCTTCCTCTGCTGAAGGAATAAATTGCTCCTTGACATTGTAGAGCTTCTGGCACTTGGAGACTTGTATGAAA
GATGGCTGTGCCTCTGCCTGTCTCCCCCACCGGGCTGGGAGCTCTGCAGAGCAGGAAACATGACTCGTATATGTCTCAGGTCCCTGCAGGGCCAAGCACC
TAGCCTCGCTCTTGGCAGGTACTCAGCGAATGAATGCTGTATATGTTGGGTGCAAAGTTCCCTACTTCCTGTGACTTCAGCTCTGTTTTACAATAAAATC
TTGAAAATGCCTATA
  • IL1RN.F5 (forward primer)
  • IL1RN.R9 (reverse primer)
  • Single exon skipping

Frame 0

CTL*GALEGGAAPPWEGLWPRYCPGATLWAAAQ
LS*SLEDLRRPPVL*GPPHGFS*LV*RRRWRRRR
R*RQC*LKGGIIKLIIKAKKARASMYR*KHKIT
A*VMTFSADS*LTHKLLGQKSSWKALNLRKDSQ
DDLPTLWEKIQQDASLQNLGC*PEDLLSEEQPTS
CWILARTKCQFRRKDRCGTH*ASCSVLGNPWRE
DVPVLCQVW**DQTPAGGS*HH*PEREQKAGQA
LRLHPLRQWPHHQF*VCRLPRLVPLHSDGS*PAR
QPHQYA*RRRHGHQILLPGGRVVLPRPACSHSC
MARTAGTASPPAPGLPAMGALRTSH*GVDPQKA
SQQPGHRTLPPLQLTSLHAASRMVFLMCESEHSS
PCTKPFHVASAFRIKPRPPAQPALLLPLPLPPS
FHLPMPWIHQAT**PPTKWLPHPVLQKRKDQSM
REVFKGLWKMKIRIS*FFFFSPREGEPFIWRLCS
FGERLRT*NIPAFVK*W*K*VVAFPFFFFFFCD
VPTCKN*KLWYYVSPIIFFFLLKHFHNLDSSVQ
ALLPSLQAPSPLQIFYSCLQYFTSYQKFLSSQGS
EQMWLLGVLSSSAEGINCSLTL*SFWHLETCMK
DGCASACLPHRAGSSAEQET*LVYVSGPCRAKH
LASLLAGTQRMNAVYVGCKVPYFL*LQLCFTIKS
*KCL

Frame 1

VLSEVLWKEGQLHPGRDCGPGTARVLLYGQQLS
*VRVWKTSEDLLSYEALPMALADLYEEGGGGGG
EGEDNADSKGGL*N*SSKPRRQEQACTAENTR*L
HK**LSVQIHS*PINCWGKNHLGRL*TSERIHK
TICRPSGRKSSKMQAFRIWDVNQKTFYLRNNQL
VAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGK
MCLSCVKSGDETRLQLEAVNITDLSENRKQDKR
FAFIRSDSGPTTSFESAACPGWFLCTAMEADQP
VSLTNMPDEGVMVTKFYFQEDE*YCPGLPVPILA
WQGLQGLPVPLPQGSRLWGH*GPAIEGWTLRRR
HNNLVTGLCLLFN*PASMLPPEWSF*CVNQSTA
APAQSPSMSPLHSGSNPDHLPNLLSSCHCLFLPH
STFPCPGSIRPLDDPQPSGSHTLFYKKEKTSP*
GRFLRVCGK*KLGFHDFFFSVPVKESPSFGDYV
LSGRG*GLKIFLHL*NDGESKW*LFPSFSSFFVM
SQLVKIKSYGTMLAP*FFFSF*NTSIIWTPLSR
HCCPASKLHLHSRFFTAACSTLPPIRSFSAPKA
LSKCGSWGFFLPLLKE*IAP*HCRASGTWRLV*K
MAVPLPVSPTGLGALQSRKHDSYMSQVPAGPST
*PRSWQVLSE*MLYMLGAKFPTSCDFSSVLQ*N
LENAY

Frame 2

YSLRCSGRRGSSTLGGTVAQVLPGCYFMGSSSV
ELESGRPQKTSCPMRPSPWL*LTCMKKEVEEEE
KVKTMLTQRGDYKTNHQSQEGKSKHVPLKTQDN
CISNDFQCRFIANP*TAGAKIILEGSEPQKGFTR
RSADPLGENPARCKPSESGMLTRRPSI*GTTN*
LLDTCKDQMSI*KKR*MWYPLSLMLCSWESMEG
RCACPVSSLVMRPDSSWRQLTSLT*ARTESRTSA
SPSSAQTVAPPPVLSLPPAPVGSSAQRWKLTSP
SASPICLTKASWSPNSTSRRTSSTAQACLFPFL
HGKDCRDCQSPCPRAPGYGGTEDQPLRGGPSEGV
TTTWSQDSASSSTDQPPCCLQNGLSNV*IRAQQ
PLHKALPCRLCIQDQTPTTCPTCSPLATASSSL
IPPSHALDPSGHLMTPNQVAPTPCFTKKKRPVHE
GGF*GFVENEN*DFMIFFFQSP*RRALHLEIMF
FRGEAEDLKYSCICEMMVKVSGSFSLLFLLFL*
CPNL*KLKVMVLC*PHNFFFPFKTLP*SGLLCPG
TAAQPPSSISTPDFLQLPAVLYLLSEVSQLPRL
*ANVAPGGSFFLC*RNKLLLDIVELLALGDLYE
RWLCLCLSPPPGWELCRAGNMTRICLRSLQGQAP
SLALGRYSANECCICWVQSSLLPVTSALFYNKI
LKMPI

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