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Sequences

Sequence ILK.gAug05

ILK.F11 (forward primer)
ILK.R12 (reverse primer)
Intron retention

Frame 0

SPSPGECERTRAPCHPSLLLSPK*GTEPTLGRT PGPLSQSIRSLNLPTSPNSVRG*GLVACSRTSVQ QTLPLRHPLPTLTRLYLASRGHSQGSSRSPELL HRPPLAGDAAQAPYPQHKHFSPVEDKAWGSSSF PGSLHSPQASPIQGTRRRDAAMDDIFTQCREGNA VAVRLWLDNTENDLNQGDDHGFSPLHWACREGR SAVVEMLIMRGARINVMNRGDDTPLHLAASHGH RDIVQKLLQYKADINAVNEHGNVPLHYACFWGQD QVAEDLVANGALVSICNKYGEMPVDKAKAPLRE LLREKMGQNLNRIPYKDTFWKGTTRTRPRESPL WEEGL*KEIILASWGWVRVKLGT*PAHTLRKWNP EQTLWH*LQTA*LPDEAQRESLWRGDPCPSCPS LTKPP*ITCFVPVLSFSSS*WARKWQQHFKPPN PYLSCSYGRAAGRAMTLS*RC*RFETGVQGRAGT SMKSVPGSGFSRIQMCSQC*VPASLHLLLILLS SHTGCRMDPSTMYYMKAPISSWTRARL*SLLWT WQGAWPSYTH*SPSSHDMHSIAVV**LMRT*LPE LAWLMSSSLSNVLVACMHLPG*PPKLCRRSLKT QTDAQQTCGVLQCFCGNW*HGRYPLLTSPIWRL E*R*EHNSIHLCCGSGW*W*K*L*WALAPHIFVR IYSNPVPRASGFSLHDRLKL*GCFFSCIRRWHW KAFGLPSHQVFPLMCVSS*RSA*MKTLQSDPNL T*LCLSLRRCRTSRTGRSLPELQRCRDMVGGMHL PKAAGLWLPPPPPVMVLPQPWGPSPSPIPTTVA PRGAGSELCHLPHGVSQHGRDQPRLSQ*SLL*K

Frame 1

ARVPVSARGPAPPVTPHFS*VPSKERSQPWGGP PVPSPRVYGA*TSPLPPTLFADRV*LPALGHPF SRHYLFVTPCPP*PAFTSRLEDTARDHPAAPNSF TDPH*PGTQLRPPTPNTNTSLL*RIKLGVHPPS LDHSTVLRLPQSRGLGAGTLLWTTFSLSAGRAT QSPFACGWTTRRTTSTRGTIMASPPCTGPAERAA LLWLRC*SCGGHGSM**TVGMTPPCIWQPVMDT VILYRSYCSTRQTSMQ*MNTGMCPCTMPVFGAK IKWQRTWWQMGPLSASVTSMERCLWTKPRHP*ES FSERRWARISTVFHTRTHSGRGPPALGPVSHHC GKKGCKRK*SWPLGAGLG*SWVPDLPTLLGNGT LNKHSGIDFKQLNFLTKLNENHSGEVTPALLALP SLNPHKLLALYLF*VFPPVSGQGSGSNISSLLT PTCPAAMEGPLAGQ*HCREGAEGSRLEYKEEQG LQ*RVSPAQDFLASKCAPSARCLPVSTCSSSYSH HTLDAVWIPLQCTT*RHQFRRGPEPGCEVCFGH GKGHGLPTHTRAPHPTTCTQ*P*CND**GHDCP N*HG*CQVLFPMSWSHVCTCLGSPRSSAEEA*RH KQTLSRHVEFCSASVGTGDTGGTLC*PLQYGDW NEGESTTAYICVAGVVGDGENNCSGPWLLTYLF GYTVILSQGPVASLYMTDSNCEAAFFLVFAGGIG RPSAYHPTRYFPSCV*AHEDLHE*RPCKATQI* HDCAYP*EDAGQVGLEGPCLNSRGVGTWLGECT SPKQQASGCLPRLQSWYYPSHGVHPLPPSLPLWP QEGRAQSFVTCHMVSPNMGGISPACHNKVYYEN

Frame 2

PESR*VREDPRPLSPLTSPESQVRNGANPGEDP RSPLPEYTEPEPPHFPQLCSRIGSSCLLSDIRS ADTTSSSPPAHPDPPLPRV*RTQPGIIPQPRTP SQTPTSRGRSSGPLPPTQTLLSCRG*SLGFILLP WITPQSSGFPNPGDSAPGRCYGRHFHSVPGGQR SRRSPVAGQHGERPQPGGRSWLLPLALGLPRGP LCCG*DVDHAGGTDQCNEPWG*HPPASGSQSWTP *YCTEAIAVQGRHQCSE*TRECAPALCLFLGPR SSGRGPGGKWGPCQHL*QVWRDACGQSQGTPER ASPREDGPESQPYSIQGHILEGDHPHSAP*VTTV GRRVVKGNNPGLLGLG*GEAGYLTCPHS*EMEP *TNTLALTSNSLTS*RSSTRITLER*PLPFLPF PH*TPINYLLCTCFKFFLQLVGKEVAATFQAS*P LPVLQLWKGRWQGNDIVVKVLKVRDWSTRKSRD FNEECPRLRIFSHPNVLPVLGACQSPPAPHPTL ITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDM ARGMAFLHTLEPLIPRHALNSRSVMIDEDMTAR ISMADVKFSFQCPGRMYAPAWVAPEALQKKPED TNRRSADMWSFAVLLWELVTREVPFADLSNMEIG MKVRAQQHTFVLREWLVMVKITVVGLGSSHICS DIQ*SCPKGQWLLST*QTQIVRLLFFLYSQVAL EGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFD MIVPILEKMQDK*DWKVLA*TPEVSGHGWGNAP PQSSRPLVASPASSHGTTPAMGSIPFPHPYHCG PKRGGLRALSLATWCLPTWEGSAPPVTIKFIMK

Sequence ILK.shed1.aAug05

AGCCCGAGTCCCGGTGAGTGCGAGAGGACCCGCGCCCCCTGTCACCCCTCACTTCTCCTGAGTCCCAAGTAAGGAACGGAGCCAACCCTGGGGAGGACCC CCGGTCCCCTCTCCCAGAGTATACGGAGCCTGAACCTCCCCACTTCCCCCAACTCTGTTCGCGGATAGGGTCTAGTTGCCTGCTCTCGGACATCCGTTCA GCAGACACTACCTCTTCGTCACCCCCTGCCCACCCTGACCCGCCTTTACCTCGCGTCTAGAGGACACAGCCAGGGATCATCCCGCAGCCCCGAACTCCTT CACAGACCCCCACTAGCCGGGGACGCAGCTCAGGCCCCCTACCCCCAACACAAACACTTCTCTCCTGTAGAGGATAAAGCTTGGGGTTCATCCTCCTTCC CTGGATCACTCCACAGTCCTCAGGCTTCCCCAATCCAGGGGACTCGGCGCCGGGACGCTGCTATGGACGACATTTTCACTCAGTGCCGGGAGGGCAACGC AGTCGCCGTTCGCCTGTGGCTGGACAACACGGAGAACGACCTCAACCAGGGGTGAGCTGAAACGGTTGGTGGATGAGAGGAAGGCTAGAGATCTCCTGGC TACGTGGAGTGGAGTGCTCATGTCTGGTGGTGGCGGCTGCCTTTGCTAGCCAGTGTAATGAGTGCTAAGCATAGCCTGTGGGGGGCAGAGGGTTTTCACA GAGGACGCAGTTTGAGCTGAATCTTGACTGCAGAATAAGAGTTTCACAGGCAGAGGCTACAGAGAGCTTTCCAGCCTCAGTAGACTGCATGGCCAAAGGC TAGGGGGCAAGGCACAGAGCAGAGAGAACAGGAAATTGTCTGGAGTTCCATATGGCTGGAGGATAGATGATCTCTGTGGGTCGGAGCATTGGGTACAAGA GGAAAAGAACAGGACATAAGACTGTAGAGGTCTCCAAGGATCAGCCAGATCATGCAAGCATATTTAGGCTATAAGAAGGTCTTTATTTTAAAAGCAAGGT TTTAAAGCTGTATAGTGACATAATGAGATTGTCCATTTTTCAGTATCGTCTTGGATGCTTTGTGAAGAGCAGGTGGAAAGGAGGCAATTGCCTAGTTCAT CGTAGAAGTAATGATGTCTTGGACTAGAATTAGGGTGAGTGAAAAAGAGAAGTGATCAAATTCCAGAAGTCTTTAGGACGAAGAATTGTGGAACTTGGTG TGGGGAGTGAGTAAGGGCAAAGATGAGGACCAGGGTTCTGGCTTGGGAACTTGGTTGTTTTTACTGAGATGGAGAAAACTGGAGGATGAGGCATTTTATT TTGGACATGTTAAAGTTAAGGTGACAATACAGCAAGGTGGAAATGTTGAGTGTGTTGTTGGTTATGCAGATTTGCAATTCAGGGGAGGAATCTGACTTGG AGACACAGATTTGAGAGCTATCAGCATATAGATAGCATATATATATCATATATATAGATGATACTTGAAGACGTGGTTGTGAAGGAGAACGTCTCAGGAG AGTGGAAGGCAAGAGGTCAGGGGACAGGAAGCCTGCACGACAGAAAAGGAGCAACCGCAAGTTGAAAAAGAAATAGGAAAATGTGTGAGTAAAGCTTACA GAAGAGAATGTTTTAATGAAGGGGAGGTAGTCAACGGCAGTACAGGCTACTGAGAGATCAAAGAACATTAATATTTTAAAGTAGCCATTGAATTTACAGT TGGGTTTTTTTTTTTTAAGTTAAGGGGTACATGTGCAGGTTTGTTGGATGGGTAAACTCAGGTCATGGAGGTTTGTTGTGCAGATCATTTCATCACCCAG GTATTAAGCCGTGGCACCCATTAGTTATTTTTCCTGATCCCTCCCTCTTCCTACCCGCCCCACTCTGGTAGGCCCCAGTGTCTGTTGCTCCCCTCAGTGT GCAGTTAGGATGTATTTGACCATCTGTGAGTGTTGTTTCAGAATAATGGGTGAAGAAATCAGACTACATTGGGTTCCTTAATTTAAGGCACTATGATGAA CACCTTCTGTGCTGGGTGCTAGAGTCCAGAATCTGACATAATCTCTGCTCTCGATACAGCAGATTTGTGGCAGATTGAAAAGTAGGCCGGGCGCGGTGGC TCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCTGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTGAAACCCTGTCTCTA CTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGTGCGCCTGTAATCCCAGCTACTCGGAGGCTGAGGCAGGAGAACTGCTTGAACCCGGGAAGCAGAGG CTGCAGTGAGCCAAGATCATGCCACTGCCCTCCAGCCTGGGCCACAGAGCAAGACTCTGTCTTAAAAAAGAAAAAGAAAACAAAAGTTGAGAGGTGAGCA AATAGTGAAAGTGAATGTACACCAGTTCTACAAGGTTGGCTGAGATAGGAAGGCAAGAAGAGCAGTAGTGATGGTGACTCAGAGTGGCAAAATCAAATTC TTTATTTTTTAAAATTGGTGGAGTTTAGTTTTTAGGTTTGACAGGTCAGGATCACCTGTCATCTTAGTCTAGACTTCACTGGTTCCCATTAGGTTCTACA CTATTTCTCTTTAATACTTTGCAAAGTCATTTGTTCCATTTTTCATATAATTCTCAAAGGGTACCCTGATAAAGACAGTAAGCACTGTTACTGTTTTGTT GGTGAGGAAATAGAGGCTTAGGCAGTTGCCCAGTGGGCCTAGCATCTAGGAAGACTCTAGCTGAGTCTAGGAAGACTCTAGGAAGATGCTGCCAGAAGGA AAAGGGGTGGAATTAATGAAACTGGAAGGTTGTGGTGCTGGTTTGAGGAGTAAAGTATGGGGGCCAAAGTTGGCTATATGCTGGATATGAAGAGGGGGTT AATTCCTTGCAGGTCTTCTTGAGATAGAAGTCCAGGCCCTGAGGTGGCAGGCAGCCTGATAGTGAACAGAACCCTTGTGCCCATACTTTTGCCCCCCGAG TTTGGCCTGCCCTGGCGCCACAGGCAGTACTGGAAGCTGATAAGTCAGGGCACAAATTAAAGGTCTAGGAGGCAAGACCAACTGATAAACAGGGGGCGTA ATGCTTCCTGGGGATAATCCCATCTCAAGGAAAAGCTTTTGGCTATTTCTGTGTTAGCAGATGCGAGCAAGTTGTGGAGGAAAATGATTAACTTAAAGGC CACACCCCCCAACCCCTTCAGCTGTACGGGCATCTGGATGGCCAGACCCCTGATCAGCTCCAGGCCTTGCCTAGAGTCGCACACAGGAAGTGACCACAAC ACTAAGGCATCCGGAGGCTGCTGGCCCAGACCACGGACCGCCCCCTGCTCCGCCCCTTGAATCTGCAGCCCCGAACTGGAGGGAATTTCCTTTTATGGCC CCAGGCTGTCAGGCAGGCCCAGCTCCAAACCAATTGTTTTGTTTTGTTTGTATACTTTACTTTATAAAATACATATTTCTTGGAAAAGAAAAAAATCAGG TATAGTTTTAATCCCAAAACAGCCATTCTCATTTTCTTCTGTTTTCTATGCACAGGATTGCTAAGTAGGGGAATCCTGCTTTGCATGGTGGTTTGTTATC TACCTCATCAGTAGTCAAAAGCATTTCTCCTTTTCAACATAAACCCTTCCAAAACATCATTTAAATTCCATTCCATTTTTAGATGTTAGTTTCAGTACCA GAGCCAAATAGTAAGTTTGTTCCCTCTTCTATAAGTTGTGGCCTGCCTTCTCCAAGACACCCTTCTCCCCCACTAACATGCACTGAACAGCTGCTGTCAA TCCATTCCCTCAAGAAACTTAAAGGTCTAACAGACAAGACAGTCCATTAACAGATTTTTACAATCCAGTGCAACAAGTGCTGAAGTAGGGGGACATATAA GATGTGGTGGAAAACAGAAAGGACCAATAGATGTTGCAGAAGGGGTGCAAGAGAAACCAGGGGAAGAGCACTACCACCTAAGGAAATGAGATGTGGGATA TGGTGAAGATAACACATTTTTTTATAGAGGTTGTTGAGATATCTAGGTGGTTGGTTTGGTTTGAAGCTCAGGGGAAGGTCTGAAATGGACAGCTTTGGGA GTTGCTGGCATGAATGAGAATCAAAGTCCTAGAGAGGTAAGCCTTCCCAGAGAGTATGTAATTATCAGTTTTTCAGGAATCAAAACCTTTGCCCCATCCC ACCTCCAGCTCAATGACCATTGCCCCTTCCTCAAAGGGACGATCATGGCTTCTCCCCCTTGCACTGGGCCTGCCGAGAGGGCCGCTCTGCTGTGGTTGAG ATGTTGATCATGCGGGGGGCACGGATCAATGTAATGAACCGTGGGGATGACACCCCCCTGCATCTGGCAGCCAGTCATGGACACCGTGATATTGTACAGA AGGTACGTACAAACTCCTTCGTCATCCACATCACATACATGCCATGAGGGTCAGTCACAGGCACTGTAACATACAGTAGAAAGCATGTGTGCTCTTCCCC CTTTTCCCATGCCCTGACACCAGTATCTCATTTGGAACTGACTGTACTTTCTGCCTCTTCTTTTTGTCTGGCCATGGGGTCCAGCTATTGCAGTACAAGG CAGACATCAATGCAGTGAATGAACACGGGAATGTGCCCCTGCACTATGCCTGTTTTTGGGGCCAAGATCAAGTGGCAGAGGTGAGTACTCAGCCCTTAAT TCCTGAGATGGGTAGGAAGTAAAGTCTGAGCCTTGGTGGGAGATTTTGGAACCCTCAACCCATTCTGTCAGTACTACTGTGTGACACTTACAGGATTAAG TTCTACATTTGTGCATTCATGGTTGGTTCAGTGACTGCCAGCGAGGTAGCAGTGGCTCTCATCATAATGGCCTTTTCATTCCAGGACCTGGTGGCAAATG GGGCCCTTGTCAGCATCTGTAACAAGTATGGAGAGATGCCTGTGGACAAAGCCAAGGCACCCCTGAGAGAGCTTCTCCGAGGTCCATCTCCCCCATCCCC TAGCTTGTGTCCTCTCGTCCCTTCCCACCTGTCTTCTCCCTCTGTACCACAGCTTAGGTTGTTTTTCTTCCCTAGAGCGGGCAGAGAAGATGGGCCAGAA TCTCAACCGTATTCCATACAAGGACACATTCTGGAAGGGGACCACCCGCACTCGGCCCCGTGAGTCACCACTGTGGGAAGAAGGGTTGTAAAAGGAAATA ATCCTGGCCTCTTGGGGCTGGGTTAGGGTGAAGCTGGGTACCTGACCTGCCCACACTCTTAGGAAATGGAACCCTGAACAAACACTCTGGCATTGACTTC AAACAGCTTAACTTCCTGACGAAGCTCAACGAGAATCACTCTGGAGAGGTGACCCCTGCCCTTCTTGCCCTTCCCTCACTAAACCCCCATAAATTACTTG CTTTGTACCTGTTTTAAGTTTTTCCTCCAGTTAGTGGGCAAGGAAGTGGCAGCAACATTTCAAGCCTCCTAACCCCTACCTGTCCTGCAGCTATGGAAGG GCCGCTGGCAGGGCAATGACATTGTCGTGAAGGTGCTGAAGGTTCGAGACTGGAGTACAAGGAAGAGCAGGGACTTCAATGAAGAGTGTCCCCGGCTCAG GTAGTGCAAGGCGTAACCTGGAAGCTGCTAGTTCCAAGGAACCCTGAATAGCACTGAAAGAGATCTTTTGTACTGGGTCTCAACCACTCCCTCCCTCTTC TAGGATTTTCTCGCATCCAAATGTGCTCCCAGTGCTAGGTGCCTGCCAGTCTCCACCTGCTCCTCATCCTACTCTCATCACACACTGGATGCCGTATGGA TCCCTCTACAATGTACTACATGAAGGCACCAGTGAGTAGGGATGTTGAATTTCCTTGGGGAGGAAATGGCAGAGAGGGAGCCTCTCTGAACTATTTGACT TTTGCCTCCTCTCAGATTTCGTCGTGGACCAGAGCCAGGCTGTGAAGTTTGCTTTGGACATGGCAAGGGGCATGGCCTTCCTACACACACTAGAGCCCCT CATCCCACGACATGCACTCAATAGCCGTAGTGTAATGGTGAGGCCACAAGCTCACTCCTGGCCCAGGCCCCAAAAGCCCTTTGCCTATCTATGACTTACC TCCTTCTATCTGTTTTCTCTTCCTCAGATTGATGAGGACATGACTGCCCGAATTAGCATGGCTGATGTCAAGTTCTCTTTCCAATGTCCTGGTCGCATGT ATGCACCTGCCTGGGTAGCCCCCGAAGGTGAGTGAAGTCATCATGTCGGGAGGTAAAAAAGGACCACCTCAGAAGTAGTGGAAGGGGGCAGAGACAGGAC AGGCAAGGGGGCCAGAACAGACAAGCCCTATCTCTCCAGCTCTGCAGAAGAAGCCTGAAGACACAAACAGACGCTCAGCAGACATGTGGAGTTTTGCAGT GCTTCTGTGGGAACTGGTGACACGGGAGGTACCCTTTGCTGACCTCTCCAATATGGAGATTGGAATGAAGGTGAGAGCACAACAGCATACATTTGTGTTG CGGGAGTGGTTGGTGATGGTGAAAATAACTGTAGTGGGCCTTGGCTCCTCACATATTTGTTCGGATATACAGTAATCCTGTCCCAAGGGCCAGTGGCTTC TCTCTACATGACAGACTCAAATTGTGAGGCTGCTTTTTTTCTTGTATTCGCAGGTGGCATTGGAAGGCCTTCGGCCTACCATCCCACCAGGTATTTCCCC TCATGTGTGTAAGCTCATGAAGATCTGCATGAATGAAGACCCTGCAAAGCGACCCAAATTTGACATGATTGTGCCTATCCTTGAGAAGATGCAGGACAAG TAGGACTGGAAGGTCCTTGCCTGAACTCCAGAGGTGTCGGGACATGGTTGGGGGAATGCACCTCCCCAAAGCAGCAGGCCTCTGGTTGCCTCCCCCGCCT CCAGTCATGGTACTACCCCAGCCATGGGGTCCATCCCCTTCCCCCATCCCTACCACTGTGGCCCCAAGAGGGGCGGGCTCAGAGCTTTGTCACTTGCCAC ATGGTGTCTCCCAACATGGGAGGGATCAGCCCCGCCTGTCACAATAAAGTTTATTATGAAAAC

ILK.F11 (forward primer)
ILK.R12 (reverse primer)
Intron retention

Frame 0

SPSPGECERTRAPCHPSLLLSPK*GTEPTLGRT PGPLSQSIRSLNLPTSPNSVRG*GLVACSRTSVQ QTLPLRHPLPTLTRLYLASRGHSQGSSRSPELL HRPPLAGDAAQAPYPQHKHFSPVEDKAWGSSSF PGSLHSPQASPIQGTRRRDAAMDDIFTQCREGNA VAVRLWLDNTENDLNQG*AETVGG*EEG*RSPG YVEWSAHVWWWRLPLLASVMSAKHSLWGAEGFH RGRSLS*ILTAE*EFHRQRLQRAFQPQ*TAWPKA RGQGTEQREQEIVWSSIWLEDR*SLWVGALGTR GKEQDIRL*RSPRISQIMQAYLGYKKVFILKAR F*SCIVT**DCPFFSIVLDAL*RAGGKEAIA*FI VEVMMSWTRIRVSEKEK*SNSRSL*DEELWNLV WGVSKGKDEDQGSGLGTWLFLLRWRKLEDEAFY FGHVKVKVTIQQGGNVECVVGYADLQFRGGI*LG DTDLRAISI*IAYIYHIYR*YLKTWL*RRTSQE SGRQEVRGQEACTTEKEQPQVEKEIGKCVSKAY RRECFNEGEVVNGSTGY*EIKEH*YFKVAIEFTV GFFFFKLRGTCAGLLDG*TQVMEVCCADHFITQ VLSRGTH*LFFLIPPSSYPPHSGRPQCLLLPSV CS*DVFDHL*VLFQNNG*RNQTTLGSLI*GTMMN TFCAGC*SPESDIISALDTADLWQIEK*AGRGG SRL*SQHFGRPRLVDHEVRSSRPA*PTW*NPVS TKNTKISQAWWRAPVIPATRRLRQENCLNPGSRG CSEPRSCHCPPAWATEQDSVLKKKKKTKVER*A NSESECTPVLQGWLR*EGKKSSSDGDSEWQNQI LYFLKLVEFSF*V*QVRITCHLSLDFTGSH*VLH YFSLILCKVICSIFHIILKGYPDKDSKHCYCFV GEEIEA*AVAQWA*HLGRL*LSLGRL*EDAARR KRGGINETGRLWCWFEE*SMGAKVGYMLDMKRGL IPCRSS*DRSPGPEVAGSLIVNRTLVPILLPPE FGLPWRHRQYWKLISQGTN*RSRRQDQLINRGR NASWG*SHLKEKLLAISVLADASKLWRKMINLKA TPPNPFSCTGIWMARPLISSRPCLESHTGSDHN TKASGGCWPRPRTAPCSAP*ICSPELEGISFYG PRLSGRPSSKPIVLFCLYTLLYKIHISWKRKKSG IVLIPKQPFSFSSVFYAQDC*VGESCFAWWFVI YLISSQKHFSFST*TLPKHHLNSIPFLDVSFST RAK**VCSLFYKLWPAFSKTPFSPTNMH*TAAVN PFPQET*RSNRQDSPLTDFYNPVQQVLK*GDI* DVVENRKDQ*MLQKGCKRNQGKSTTT*GNEMWD MVKITHFFIEVVEISRWLVWFEAQGKV*NGQLWE LLA*MRIKVLER*AFPESM*LSVFQESKPLPHP TSSSMTIAPSSKGRSWLLPLALGLPRGPLCCG* DVDHAGGTDQCNEPWG*HPPASGSQSWTP*YCTE GTYKLLRHPHHIHAMRVSHRHCNIQ*KACVLFP LFPCPDTSISFGTDCTFCLFFLSGHGVQLLQYK ADINAVNEHGNVPLHYACFWGQDQVAEVSTQPLI PEMGRK*SLSLGGRFWNPQPILSVLLCDTYRIK FYICAFMVGSVTASEVAVALIIMAFSFQDLVAN GALVSICNKYGEMPVDKAKAPLRELLRGPSPPSP SLCPLVPSHLSSPSVPQLRLFFFPRAGREDGPE SQPYSIQGHILEGDHPHSAP*VTTVGRRVVKGN NPGLLGLG*GEAGYLTCPHS*EMEP*TNTLALTS NSLTS*RSSTRITLER*PLPFLPFPH*TPINYL LCTCFKFFLQLVGKEVAATFQAS*PLPVLQLWK GRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLR *CKA*PGSC*FQGTLNSTERDLLYWVSTTPSLF *DFLASKCAPSARCLPVSTCSSSYSHHTLDAVW IPLQCTT*RHQ*VGMLNFLGEEMAEREPL*TI*L LPPLRFRRGPEPGCEVCFGHGKGHGLPTHTRAP HPTTCTQ*P*CNGEATSSLLAQAPKALCLSMTY LLLSVFSSSD**GHDCPN*HG*CQVLFPMSWSHV CTCLGSPRR*VKSSCREVKKDHLRSSGRGQRQD RQGGQNRQALSLQLCRRSLKTQTDAQQTCGVLQ CFCGNW*HGRYPLLTSPIWRLE*R*EHNSIHLCC GSGW*W*K*L*WALAPHIFVRIYSNPVPRASGF SLHDRLKL*GCFFSCIRRWHWKAFGLPSHQVFP LMCVSS*RSA*MKTLQSDPNLT*LCLSLRRCRTS RTGRSLPELQRCRDMVGGMHLPKAAGLWLPPPP PVMVLPQPWGPSPSPIPTTVAPRGAGSELCHLP HGVSQHGRDQPRLSQ*SLL*K

Frame 1

ARVPVSARGPAPPVTPHFS*VPSKERSQPWGGP PVPSPRVYGA*TSPLPPTLFADRV*LPALGHPF SRHYLFVTPCPP*PAFTSRLEDTARDHPAAPNSF TDPH*PGTQLRPPTPNTNTSLL*RIKLGVHPPS LDHSTVLRLPQSRGLGAGTLLWTTFSLSAGRAT QSPFACGWTTRRTTSTRGELKRLVDERKARDLLA TWSGVLMSGGGGCLC*PV**VLSIACGGQRVFT EDAV*AES*LQNKSFTGRGYRELSSLSRLHGQR LGGKAQSRENRKLSGVPYGWRIDDLCGSEHWVQE EKNRT*DCRGLQGSARSCKHI*AIRRSLF*KQG FKAV**HNEIVHFSVSSWMLCEEQVERRQLPSS S*K**CLGLELG*VKKRSDQIPEVFRTKNCGTWC GE*VRAKMRTRVLAWELGCFY*DGENWRMRHFI LDMLKLR*QYSKVEMLSVLLVMQICNSGEESDL ETQI*ELSAYR*HIYIIYIDDT*RRGCEGERLRR VEGKRSGDRKPARQKRSNRKLKKK*ENV*VKLT EENVLMKGR*STAVQATERSKNINILK*PLNLQ LGFFFLS*GVHVQVCWMGKLRSWRFVVQIISSPR Y*AVAPISYFS*SLPLPTRPTLVGPSVCCSPQC AVRMYLTICECCFRIMGEEIRLHWVP*FKAL** TPSVLGARVQNLT*SLLSIQQICGRLKSRPGAVA HACNPSTLGGRGWWITRSGVRDQPDQHGETLSL LKIQKLARRGGVRL*SQLLGG*GRRTA*TREAE AAVSQDHATALQPGPQSKTLS*KRKRKQKLRGEQ IVKVNVHQFYKVG*DRKARRAVVMVTQSGKIKF FIF*NWWSLVFRFDRSGSPVILV*TSLVPIRFY TISL*YFAKSFVPFFI*FSKGTLIKTVSTVTVLL VRK*RLRQLPSGPSI*EDSS*V*EDSRKMLPEG KGVELMKLEGCGAGLRSKVWGPKLAICWI*RGG *FLAGLLEIEVQALRWQAA***TEPLCPYFCPPS LACPGATGSTGS**VRAQIKGLGGKTN**TGGV MLPGDNPISRKSFWLFLC*QMRASCGGK*LT*R PHPPTPSAVRASGWPDP*SAPGLA*SRTQEVTTT LRHPEAAGPDHGPPPAPPLESAAPNWREFPFMA PGCQAGPAPNQLFCFVCILYFIKYIFLGKEKNQ V*F*SQNSHSHFLLFSMHRIAK*GNPALHGGLLS TSSVVKSISPFQHKPFQNII*IPFHF*MLVSVP EPNSKFVPSSISCGLPSPRHPSPPLTCTEQLLS IHSLKKLKGLTDKTVH*QIFTIQCNKC*SRGTYK MWWKTERTNRCCRRGARETRGRALPPKEMRCGI W*R*HIFL*RLLRYLGGWFGLKLRGRSEMDSFG SCWHE*ESKS*RGKPSQRVCNYQFFRNQNLCPIP PPAQ*PLPLPQRDDHGFSPLHWACREGRSAVVE MLIMRGARINVMNRGDDTPLHLAASHGHRDIVQ KVRTNSFVIHITYMP*GSVTGTVTYSRKHVCSSP FSHALTPVSHLELTVLSASSFCLAMGSSYCSTR QTSMQ*MNTGMCPCTMPVFGAKIKWQR*VLSP* FLRWVGSKV*ALVGDFGTLNPFCQYYCVTLTGLS STFVHSWLVQ*LPAR*QWLSS*WPFHSRTWWQM GPLSASVTSMERCLWTKPRHP*ESFSEVHLPHP LACVLSSLPTCLLPLYHSLGCFSSLERAEKMGQN LNRIPYKDTFWKGTTRTRPRESPLWEEGL*KEI ILASWGWVRVKLGT*PAHTLRKWNPEQTLWH*L QTA*LPDEAQRESLWRGDPCPSCPSLTKPP*ITC FVPVLSFSSS*WARKWQQHFKPPNPYLSCSYGR AAGRAMTLS*RC*RFETGVQGRAGTSMKSVPGS GSARRNLEAASSKEP*IALKEIFCTGSQPLPPSS RIFSHPNVLPVLGACQSPPAPHPTLITHWMPYG SLYNVLHEGTSE*GC*ISLGRKWQRGSLSELFD FCLLSDFVVDQSQAVKFALDMARGMAFLHTLEPL IPRHALNSRSVMVRPQAHSWPRPQKPFAYL*LT SFYLFSLPQIDEDMTARISMADVKFSFQCPGRM YAPAWVAPEGE*SHHVGR*KRTTSEVVEGGRDRT GKGARTDKPYLSSSAEEA*RHKQTLSRHVEFCS ASVGTGDTGGTLC*PLQYGDWNEGESTTAYICV AGVVGDGENNCSGPWLLTYLFGYTVILSQGPVAS LYMTDSNCEAAFFLVFAGGIGRPSAYHPTRYFP SCV*AHEDLHE*RPCKATQI*HDCAYP*EDAGQ VGLEGPCLNSRGVGTWLGECTSPKQQASGCLPRL QSWYYPSHGVHPLPPSLPLWPQEGRAQSFVTCH MVSPNMGGISPACHNKVYYEN

Frame 2

PESR*VREDPRPLSPLTSPESQVRNGANPGEDP RSPLPEYTEPEPPHFPQLCSRIGSSCLLSDIRS ADTTSSSPPAHPDPPLPRV*RTQPGIIPQPRTP SQTPTSRGRSSGPLPPTQTLLSCRG*SLGFILLP WITPQSSGFPNPGDSAPGRCYGRHFHSVPGGQR SRRSPVAGQHGERPQPGVS*NGWWMRGRLEISW LRGVECSCLVVAAAFASQCNEC*A*PVGGRGFSQ RTQFELNLDCRIRVSQAEATESFPASVDCMAKG *GARHRAERTGNCLEFHMAGG*MISVGRSIGYK RKRTGHKTVEVSKDQPDHASIFRL*EGLYFKSKV LKLYSDIMRLSIFQYRLGCFVKSRWKGGNCLVH RRSNDVLD*N*GE*KREVIKFQKSLGRRIVELG VGSE*GQR*GPGFWLGNLVVFTEMEKTGG*GILF WTC*S*GDNTARWKC*VCCWLCRFAIQGRNLTW RHRFESYQHIDSIYISYI*MILEDVVVKENVSG EWKARGQGTGSLHDRKGATAS*KRNRKMCE*SLQ KRMF**RGGSQRQYRLLRDQRTLIF*SSH*IYS WVFFF*VKGYMCRFVGWVNSGHGGLLCRSFHHP GIKPWHPLVIFPDPSLFLPAPLW*APVSVAPLSV QLGCI*PSVSVVSE*WVKKSDYIGFLNLRHYDE HLLCWVLESRI*HNLCSRYSRFVAD*KVGRARW LTPVIPALWEAEAGGSRGQEFETSLTNMVKPCLY *KYKN*PGVVACACNPSYSEAEAGELLEPGKQR LQ*AKIMPLPSSLGHRARLCLKKEKENKS*EVS K**K*MYTSSTRLAEIGRQEEQ**W*LRVAKSNS LFFKIGGV*FLGLTGQDHLSS*SRLHWFPLGST LFLFNTLQSHLFHFSYNSQRVP**RQ*ALLLFC W*GNRGLGSCPVGLASRKTLAESRKTLGRCCQKE KGWN**NWKVVVLV*GVKYGGQSWLYAGYEEGV NSLQVFLR*KSRP*GGRQPDSEQNPCAHTFAPR VWPALAPQAVLEADKSGHKLKV*EARPTDKQGA* CFLGIIPSQGKAFGYFCVSRCEQVVEEND*LKG HTPQPLQLYGHLDGQTPDQLQALPRVAHRK*PQ H*GIRRLLAQTTDRPLLRPLNLQPRTGGNFLLWP QAVRQAQLQTNCFVLFVYFTL*NTYFLEKKKIR YSFNPKTAILIFFCFLCTGLLSRGILLCMVVCY LPHQ*SKAFLLFNINPSKTSFKFHSIFRC*FQYQ SQIVSLFPLL*VVACLLQDTLLPH*HALNSCCQ SIPSRNLKV*QTRQSINRFLQSSATSAEVGGHI RCGGKQKGPIDVAEGVQEKPGEEHYHLRK*DVGY GEDNTFFYRGC*DI*VVGLV*SSGEGLKWTALG VAGMNENQSPREVSLPREYVIISFSGIKTFAPS HLQLNDHCPFLKGTIMASPPCTGPAERAALLWLR C*SCGGHGSM**TVGMTPPCIWQPVMDTVILYR RYVQTPSSSTSHTCHEGQSQAL*HTVESMCALP PFPMP*HQYLIWN*LYFLPLLFVWPWGPAIAVQG RHQCSE*TRECAPALCLFLGPRSSGRGEYSALN S*DG*EVKSEPWWEILEPSTHSVSTTV*HLQD* VLHLCIHGWFSDCQRGSSGSHHNGLFIPGPGGKW GPCQHL*QVWRDACGQSQGTPERASPRSISPIP *LVSSRPFPPVFSLCTTA*VVFLP*SGQRRWAR ISTVFHTRTHSGRGPPALGPVSHHCGKKGCKRK* SWPLGAGLG*SWVPDLPTLLGNGTLNKHSGIDF KQLNFLTKLNENHSGEVTPALLALPSLNPHKLL ALYLF*VFPPVSGQGSGSNISSLLTPTCPAAMEG PLAGQ*HCREGAEGSRLEYKEEQGLQ*RVSPAQ VVQGVTWKLLVPRNPE*H*KRSFVLGLNHSLPL LGFSRIQMCSQC*VPASLHLLLILLSSHTGCRMD PSTMYYMKAPVSRDVEFPWGGNGREGASLNYLT FASSQISSWTRARL*SLLWTWQGAWPSYTH*SP SSHDMHSIAVV*W*GHKLTPGPGPKSPLPIYDLP PSICFLFLRLMRT*LPELAWLMSSSLSNVLVAC MHLPG*PPKVSEVIMSGGKKGPPQK*WKGAETG QARGPEQTSPISPALQKKPEDTNRRSADMWSFAV LLWELVTREVPFADLSNMEIGMKVRAQQHTFVL REWLVMVKITVVGLGSSHICSDIQ*SCPKGQWL LST*QTQIVRLLFFLYSQVALEGLRPTIPPGISP HVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK *DWKVLA*TPEVSGHGWGNAPPQSSRPLVASPA SSHGTTPAMGSIPFPHPYHCGPKRGGLRALSLAT WCLPTWEGSAPPVTIKFIMK

Sequence ILK.jAug05

AAGAGCGCCACGGGCGGGGCGGGGCCGGCGGCGGGCTGCGGGCGCGGCCGGACGGGAGTTCCCCGGAGAAGGATCCTGCAGCCCGAGTCCCGAGGATAAA GCTTGGGGTTCATCCTCCTTCCCTGGATCACTCCACAGTCCTCAGGCTTCCCCAATCCAGGGGACTCGGCGCCGGGACGCTGCTATGGACGACATTTTCA CTCAGTGCCGGGAGGGCAACGCAGTCGCCGTTCGCCTGTGGCTGGACAACACGGAGAACGACCTCAACCAGGGGGACGATCATGGCTTCTCCCCCTTGCA CTGGGCCTGCCGAGAGGGCCGCTCTGCTGTGGTTGAGATGTTGATCATGCGGGGGGCACGGATCAATGTAATGAACCGTGGGGATGACACCCCCCTGCAT CTGGCAGCCAGTCATGGACACCGTGATATTGTACAGAAGCTATTGCAGTACAAGGCAGACATCAATGCAGTGAATGAACACGGGAATGTGCCCCTGCACT ATGCCTGTTTTTGGGGCCAAGATCAAGTGGCAGAGGACCTGGTGGCAAATGGGGCCCTTGTCAGCATCTGTAACAAGTATGGAGAGATGCCTGTGGACAA AGCCAAGGCACCCCTGAGAGAGCTTCTCCGAGGTCCATCTCCCCCATCCCCTAGCTTGTGTCCTCTCGTCCCTTCCCACCTGTCTTCTCCCTCTGTACCA CAGCTTAGGTTGTTTTTCTTCCCTAGAGCGGGCAGAGAAGATGGGCCAGAATCTCAACCGTATTCCATACAAGGACACATTCTGGAAGGGGACCACCCGC ACTCGGCCCCGTGAGTCACCACTGTGGGAAGAAGGGTTGTAAAAGGAAATAATCCTGGCCTCTTGGGGCTGGGTTAGGGTGAAGCTGGGTACCTGACCTG CCCACACTCTTAGGAAATGGAACCCTGAACAAACACTCTGGCATTGACTTCAAACAGCTTAACTTCCTGACGAAGCTCAACGAGAATCACTCTGGAGAGG TGACCCCTGCCCTTCTTGCCCTTCCCTCACTAAACCCCCATAAATTACTTGCTTTGTACCTGTTTTAAGTTTTTCCTCCAGTTAGTGGGCAAGGAAGTGG CAGCAACATTTCAAGCCTCCTAACCCCTACCTGTCCTGCAGCTATGGAAGGGCCGCTGGCAGGGCAATGACATTGTCGTGAAGGTGCTGAAGGTTCGAGA CTGGAGTACAAGGAAGAGCAGGGACTTCAATGAAGAGTGTCCCCGGCTCAGGTAGTGCAAGGCGTAACCTGGAAGCTGCTAGTTCCAAGGAACCCTGAAT AGCACTGAAAGAGATCTTTTGTACTGGGTCTCAACCACTCCCTCCCTCTTCTAGGATTTTCTCGCATCCAAATGTGCTCCCAGTGCTAGGTGCCTGCCAG TCTCCACCTGCTCCTCATCCTACTCTCATCACACACTGGATGCCGTATGGATCCCTCTACAATGTACTACATGAAGGCACCAGTGAGTAGGGATGTTGAA TTTCCTTGGGGAGGAAATGGCAGAGAGGGAGCCTCTCTGAACTATTTGACTTTTGCCTCCTCTCAGATTTCGTCGTGGACCAGAGCCAGGCTGTGAAGTT TGCTTTGGACATGGCAAGGGGCATGGCCTTCCTACACACACTAGAGCCCCTCATCCCACGACATGCACTCAATAGCCGTAGTGTAATGGTGAGGCCACAA GCTCACTCCTGGCCCAGGCCCCAAAAGCCCTTTGCCTATCTATGACTTACCTCCTTCTATCTGTTTTCTCTTCCTCAGATTGATGAGGACATGACTGCCC GAATTAGCATGGCTGATGTCAAGTTCTCTTTCCAATGTCCTGGTCGCATGTATGCACCTGCCTGGGTAGCCCCCGAAGGTGAGTGAAGTCATCATGTCGG GAGGTAAAAAAGGACCACCTCAGAAGTAGTGGAAGGGGGCAGAGACAGGACAGGCAAGGGGGCCAGAACAGACAAGCCCTATCTCTCCAGCTCTGCAGAA GAAGCCTGAAGACACAAACAGACGCTCAGCAGACATGTGGAGTTTTGCAGTGCTTCTGTGGGAACTGGTGACACGGGAGGTACCCTTTGCTGACCTCTCC AATATGGAGATTGGAATGAAGGTGAGAGCACAACAGCATACATTTGTGTTGCGGGAGTGGTTGGTGATGGTGAAAATAACTGTAGTGGGCCTTGGCTCCT CACATATTTGTTCGGATATACAGTAATCCTGTCCCAAGGGCCAGTGGCTTCTCTCTACATGACAGACTCAAATTGTGAGGCTGCTTTTTTTCTTGTATTC GCAGGTGGCATTGGAAGGCCTTCGGCCTACCATCCCACCAGGTATTTCCCCTCATGTGTGTAAGCTCATGAAGATCTGCATGAATGAAGACCCTGCAAAG CGACCCAAATTTGACATGATTGTGCCTATCCTTGAGAAGATGCAGGACAAGTAGGACTGGAAGGTCCTTGCCTGAACTCCAGAGGTGTCGGGACATGGTT GGGGGAATGCACCTCCCCAAAGCAGCAGGCCTCTGGTTGCCTCCCCCGCCTCCAGTCATGGTACTACCCCAGCCATGGGGTCCATCCCCTTCCCCCATCC CTACCACTGTGGCCCCAAGAGGGGCGGGCTCAGAGCTTTGTCACTTGCCACATGGTGTCTCCCAACATGGGAGGGATCAGCCCCGCCTGTCACAATAAAG TTTATTATGAAAAC

ILK.F11 (forward primer)
ILK.R12 (reverse primer)
Intron retention

Frame 0

KSATGGAGPAAGCGRGRTGVPRRRILQPESRG* SLGFILLPWITPQSSGFPNPGDSAPGRCYGRHFH SVPGGQRSRRSPVAGQHGERPQPGGRSWLLPLA LGLPRGPLCCG*DVDHAGGTDQCNEPWG*HPPA SGSQSWTP*YCTEAIAVQGRHQCSE*TRECAPAL CLFLGPRSSGRGPGGKWGPCQHL*QVWRDACGQ SQGTPERASPRSISPIP*LVSSRPFPPVFSLCT TA*VVFLP*SGQRRWARISTVFHTRTHSGRGPPA LGPVSHHCGKKGCKRK*SWPLGAGLG*SWVPDL PTLLGNGTLNKHSGIDFKQLNFLTKLNENHSGE VTPALLALPSLNPHKLLALYLF*VFPPVSGQGSG SNISSLLTPTCPAAMEGPLAGQ*HCREGAEGSR LEYKEEQGLQ*RVSPAQVVQGVTWKLLVPRNPE *H*KRSFVLGLNHSLPLLGFSRIQMCSQC*VPAS LHLLLILLSSHTGCRMDPSTMYYMKAPVSRDVE FPWGGNGREGASLNYLTFASSQISSWTRARL*S LLWTWQGAWPSYTH*SPSSHDMHSIAVV*W*GHK LTPGPGPKSPLPIYDLPPSICFLFLRLMRT*LP ELAWLMSSSLSNVLVACMHLPG*PPKVSEVIMS GGKKGPPQK*WKGAETGQARGPEQTSPISPALQK KPEDTNRRSADMWSFAVLLWELVTREVPFADLS NMEIGMKVRAQQHTFVLREWLVMVKITVVGLGS SHICSDIQ*SCPKGQWLLST*QTQIVRLLFFLYS QVALEGLRPTIPPGISPHVCKLMKICMNEDPAK RPKFDMIVPILEKMQDK*DWKVLA*TPEVSGHG WGNAPPQSSRPLVASPASSHGTTPAMGSIPFPHP YHCGPKRGGLRALSLATWCLPTWEGSAPPVTIK FIMK

Frame 1

RAPRAGRGRRRAAGAAGREFPGEGSCSPSPEDK AWGSSSFPGSLHSPQASPIQGTRRRDAAMDDIF TQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLH WACREGRSAVVEMLIMRGARINVMNRGDDTPLH LAASHGHRDIVQKLLQYKADINAVNEHGNVPLH YACFWGQDQVAEDLVANGALVSICNKYGEMPVDK AKAPLRELLRGPSPPSPSLCPLVPSHLSSPSVP QLRLFFFPRAGREDGPESQPYSIQGHILEGDHP HSAP*VTTVGRRVVKGNNPGLLGLG*GEAGYLTC PHS*EMEP*TNTLALTSNSLTS*RSSTRITLER *PLPFLPFPH*TPINYLLCTCFKFFLQLVGKEV AATFQAS*PLPVLQLWKGRWQGNDIVVKVLKVRD WSTRKSRDFNEECPRLR*CKA*PGSC*FQGTLN STERDLLYWVSTTPSLF*DFLASKCAPSARCLP VSTCSSSYSHHTLDAVWIPLQCTT*RHQ*VGMLN FLGEEMAEREPL*TI*LLPPLRFRRGPEPGCEV CFGHGKGHGLPTHTRAPHPTTCTQ*P*CNGEAT SSLLAQAPKALCLSMTYLLLSVFSSSD**GHDCP N*HG*CQVLFPMSWSHVCTCLGSPRR*VKSSCR EVKKDHLRSSGRGQRQDRQGGQNRQALSLQLCR RSLKTQTDAQQTCGVLQCFCGNW*HGRYPLLTSP IWRLE*R*EHNSIHLCCGSGW*W*K*L*WALAP HIFVRIYSNPVPRASGFSLHDRLKL*GCFFSCI RRWHWKAFGLPSHQVFPLMCVSS*RSA*MKTLQS DPNLT*LCLSLRRCRTSRTGRSLPELQRCRDMV GGMHLPKAAGLWLPPPPPVMVLPQPWGPSPSPI PTTVAPRGAGSELCHLPHGVSQHGRDQPRLSQ*S LL*K

Frame 2

ERHGRGGAGGGLRARPDGSSPEKDPAARVPRIK LGVHPPSLDHSTVLRLPQSRGLGAGTLLWTTFS LSAGRATQSPFACGWTTRRTTSTRGTIMASPPC TGPAERAALLWLRC*SCGGHGSM**TVGMTPPCI WQPVMDTVILYRSYCSTRQTSMQ*MNTGMCPCT MPVFGAKIKWQRTWWQMGPLSASVTSMERCLWT KPRHP*ESFSEVHLPHPLACVLSSLPTCLLPLYH SLGCFSSLERAEKMGQNLNRIPYKDTFWKGTTR TRPRESPLWEEGL*KEIILASWGWVRVKLGT*P AHTLRKWNPEQTLWH*LQTA*LPDEAQRESLWRG DPCPSCPSLTKPP*ITCFVPVLSFSSS*WARKW QQHFKPPNPYLSCSYGRAAGRAMTLS*RC*RFE TGVQGRAGTSMKSVPGSGSARRNLEAASSKEP*I ALKEIFCTGSQPLPPSSRIFSHPNVLPVLGACQ SPPAPHPTLITHWMPYGSLYNVLHEGTSE*GC* ISLGRKWQRGSLSELFDFCLLSDFVVDQSQAVKF ALDMARGMAFLHTLEPLIPRHALNSRSVMVRPQ AHSWPRPQKPFAYL*LTSFYLFSLPQIDEDMTA RISMADVKFSFQCPGRMYAPAWVAPEGE*SHHVG R*KRTTSEVVEGGRDRTGKGARTDKPYLSSSAE EA*RHKQTLSRHVEFCSASVGTGDTGGTLC*PL QYGDWNEGESTTAYICVAGVVGDGENNCSGPWLL TYLFGYTVILSQGPVASLYMTDSNCEAAFFLVF AGGIGRPSAYHPTRYFPSCV*AHEDLHE*RPCK ATQI*HDCAYP*EDAGQVGLEGPCLNSRGVGTWL GECTSPKQQASGCLPRLQSWYYPSHGVHPLPPS LPLWPQEGRAQSFVTCHMVSPNMGGISPACHNK VYYEN

Sequence ILK.eAug05

AAGAGCGCCACGGGCGGGGCGGGGCCGGCGGCGGGCTGCGGGCGCGGCCGGACGGGAGTTCCCCGGAGAAGGATCCTGCAGCCCGAGTCCCGTCCTCAGG CTTCCCCAATCCAGGGGACTCGGCGCCGGGACGCTGCTATGGACGACATTTTCACTCAGTGCCGGGAGGGCAACGCAGTCGCCGTTCGCCTGTGGCTGGA CAACACGGAGAACGACCTCAACCAGGGGGACGATCATGGCTTCTCCCCCTTGCACTGGGCCTGCCGAGAGGGCCGCTCTGCTGTGGTTGAGATGTTGATC ATGCGGGGGGCACGGATCAATGTAATGAACCGTGGGGATGACACCCCCCTGCATCTGGCAGCCAGTCATGGACACCGTGATATTGTACAGAAGCTATTGC AGTACAAGGCAGACATCAATGCAGTGAATGAACACGGGAATGTGCCCCTGCACTATGCCTGTTTTTGGGGCCAAGATCAAGTGGCAGAGGACCTGGTGGC AAATGGGGCCCTTGTCAGCATCTGTAACAAGTATGGAGAGATGCCTGTGGACAAAGCCAAGGCACCCCTGAGAGAGCTTCTCCGAGGTCCATCTCCCCCA TCCCCTAGCTTGTGTCCTCTCGTCCCTTCCCACCTGTCTTCTCCCTCTGTACCACAGCTTAGGTTGTTTTTCTTCCCTAGAGCGGGCAGAGAAGATGGGC CAGAATCTCAACCGTATTCCATACAAGGACACATTCTGGAAGGGGACCACCCGCACTCGGCCCCGAAATGGAACCCTGAACAAACACTCTGGCATTGACT TCAAACAGCTTAACTTCCTGACGAAGCTCAACGAGAATCACTCTGGAGAGCTATGGAAGGGCCGCTGGCAGGGCAATGACATTGTCGTGAAGGTGCTGAA GGTTCGAGACTGGAGTACAAGGAAGAGCAGGGACTTCAATGAAGAGTGTCCCCGGCTCAGGATTTTCTCGCATCCAAATGTGCTCCCAGTGCTAGGTGCC TGCCAGTCTCCACCTGCTCCTCATCCTACTCTCATCACACACTGGATGCCGTATGGATCCCTCTACAATGTACTACATGAAGGCACCAATTTCGTCGTGG ACCAGAGCCAGGCTGTGAAGTTTGCTTTGGACATGGCAAGGGGCATGGCCTTCCTACACACACTAGAGCCCCTCATCCCACGACATGCACTCAATAGCCG TAGTGTAATGATTGATGAGGACATGACTGCCCGAATTAGCATGGCTGATGTCAAGTTCTCTTTCCAATGTCCTGGTCGCATGTATGCACCTGCCTGGGTA GCCCCCGAAGCTCTGCAGAAGAAGCCTGAAGACACAAACAGACGCTCAGCAGACATGTGGAGTTTTGCAGTGCTTCTGTGGGAACTGGTGACACGGGAGG TACCCTTTGCTGACCTCTCCAATATGGAGATTGGAATGAAGGTGGCATTGGAAGGCCTTCGGCCTACCATCCCACCAGGTATTTCCCCTCATGTGTGTAA GCTCATGAAGATCTGCATGAATGAAGACCCTGCAAAGCGACCCAAATTTGACATGATTGTGCCTATCCTTGAGAAGATGCAGGACAAGTAGGACTGGAAG GTCCTTGCCTGAACTCCAGAGGTGTCGGGACATGGTTGGGGGAATGCACCTCCCCAAAGCAGCAGGCCTCTGGTTGCCTCCCCCGCCTCCAGTCATGGTA CTACCCCAGCCATGGGGTCCATCCCCTTCCCCCATCCCTACCACTGTGGCCCCAAGAGGGGCGGGCTCAGAGCTTTGTCACTTGCCACATGGTGTCTCCC AACATGGGAGGGATCAGCCCCGCCTGTCACAATAAAGTTTATTATGAAAACAGGCTG

ILK.F11 (forward primer)
ILK.R12 (reverse primer)
Intron retention

Frame 0

KSATGGAGPAAGCGRGRTGVPRRRILQPESRPQ ASPIQGTRRRDAAMDDIFTQCREGNAVAVRLWLD NTENDLNQGDDHGFSPLHWACREGRSAVVEMLI MRGARINVMNRGDDTPLHLAASHGHRDIVQKLL QYKADINAVNEHGNVPLHYACFWGQDQVAEDLVA NGALVSICNKYGEMPVDKAKAPLRELLRGPSPP SPSLCPLVPSHLSSPSVPQLRLFFFPRAGREDG PESQPYSIQGHILEGDHPHSAPKWNPEQTLWH*L QTA*LPDEAQRESLWRAMEGPLAGQ*HCREGAE GSRLEYKEEQGLQ*RVSPAQDFLASKCAPSARC LPVSTCSSSYSHHTLDAVWIPLQCTT*RHQFRRG PEPGCEVCFGHGKGHGLPTHTRAPHPTTCTQ*P *CND**GHDCPN*HG*CQVLFPMSWSHVCTCLG SPRSSAEEA*RHKQTLSRHVEFCSASVGTGDTGG TLC*PLQYGDWNEGGIGRPSAYHPTRYFPSCV* AHEDLHE*RPCKATQI*HDCAYP*EDAGQVGLE GPCLNSRGVGTWLGECTSPKQQASGCLPRLQSWY YPSHGVHPLPPSLPLWPQEGRAQSFVTCHMVSP NMGGISPACHNKVYYENRL

Frame 1

RAPRAGRGRRRAAGAAGREFPGEGSCSPSPVLR LPQSRGLGAGTLLWTTFSLSAGRATQSPFACGW TTRRTTSTRGTIMASPPCTGPAERAALLWLRC*S CGGHGSM**TVGMTPPCIWQPVMDTVILYRSYC STRQTSMQ*MNTGMCPCTMPVFGAKIKWQRTWW QMGPLSASVTSMERCLWTKPRHP*ESFSEVHLPH PLACVLSSLPTCLLPLYHSLGCFSSLERAEKMG QNLNRIPYKDTFWKGTTRTRPRNGTLNKHSGID FKQLNFLTKLNENHSGELWKGRWQGNDIVVKVLK VRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGA CQSPPAPHPTLITHWMPYGSLYNVLHEGTNFVV DQSQAVKFALDMARGMAFLHTLEPLIPRHALNSR SVMIDEDMTARISMADVKFSFQCPGRMYAPAWV APEALQKKPEDTNRRSADMWSFAVLLWELVTRE VPFADLSNMEIGMKVALEGLRPTIPPGISPHVCK LMKICMNEDPAKRPKFDMIVPILEKMQDK*DWK VLA*TPEVSGHGWGNAPPQSSRPLVASPASSHG TTPAMGSIPFPHPYHCGPKRGGLRALSLATWCLP TWEGSAPPVTIKFIMKTG

Frame 2

ERHGRGGAGGGLRARPDGSSPEKDPAARVPSSG FPNPGDSAPGRCYGRHFHSVPGGQRSRRSPVAG QHGERPQPGGRSWLLPLALGLPRGPLCCG*DVD HAGGTDQCNEPWG*HPPASGSQSWTP*YCTEAIA VQGRHQCSE*TRECAPALCLFLGPRSSGRGPGG KWGPCQHL*QVWRDACGQSQGTPERASPRSISP IP*LVSSRPFPPVFSLCTTA*VVFLP*SGQRRWA RISTVFHTRTHSGRGPPALGPEMEP*TNTLALT SNSLTS*RSSTRITLESYGRAAGRAMTLS*RC* RFETGVQGRAGTSMKSVPGSGFSRIQMCSQC*VP ASLHLLLILLSSHTGCRMDPSTMYYMKAPISSW TRARL*SLLWTWQGAWPSYTH*SPSSHDMHSIA VV**LMRT*LPELAWLMSSSLSNVLVACMHLPG* PPKLCRRSLKTQTDAQQTCGVLQCFCGNW*HGR YPLLTSPIWRLE*RWHWKAFGLPSHQVFPLMCV SS*RSA*MKTLQSDPNLT*LCLSLRRCRTSRTGR SLPELQRCRDMVGGMHLPKAAGLWLPPPPPVMV LPQPWGPSPSPIPTTVAPRGAGSELCHLPHGVS QHGRDQPRLSQ*SLL*KQA