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Sequences

Sequence ILK.qAug05

AGCCCGAGTCCCGGTGAGTGCGAGAGGACCCGCGCCCCCTGTCACCCCTCACTTCTCCTGAGTCCCAAGTAAGGAACGGAGCCAACCCTGGGGAGGACCC CCGGTCCCCTCTCCCAGAGTATACGGAGCCTGAACCTCCCCACTTCCCCCAACTCTGTTCGCGGATAGGGTCTAGTTGCCTGCTCTCGGACATCCGTTCA GCAGACACTACCTCTTCGTCACCCCCTGCCCACCCTGACCCGCCTTTACCTCGCGTCTAGAGGACACAGCCAGGGATCATCCCGCAGCCCCGAACTCCTT CACAGACCCCCACTAGCCGGGGACGCAGCTCAGGCCCCCTACCCCCAACACAAACACTTCTCTCCTGTAGAGGATAAAGCTTGGGGTTCATCCTCCTTCC CTGGATCACTCCACAGTCCTCAGGCTTCCCCAATCCAGGGGACTCGGCGCCGGGACGCTGCTATGGACGACATTTTCACTCAGTGCCGGGAGGGCAACGC AGTCGCCGTTCGCCTGTGGCTGGACAACACGGAGAACGACCTCAACCAGGGGTGAGCTGAAACGGTTGGTGGATGAGAGGAAGGCTAGAGATCTCCTGGC TACGTGGAGTGGAGTGCTCATGTCTGGTGGTGGCGGCTGCCTTTGCTAGCCAGTGTAATGAGTGCTAAGCATAGCCTGTGGGGGGCAGAGGGTTTTCACA GAGGACGCAGTTTGAGCTGAATCTTGACTGCAGAATAAGAGTTTCACAGGCAGAGGCTACAGAGAGCTTTCCAGCCTCAGTAGACTGCATGGCCAAAGGC TAGGGGGCAAGGCACAGAGCAGAGAGAACAGGAAATTGTCTGGAGTTCCATATGGCTGGAGGATAGATGATCTCTGTGGGTCGGAGCATTGGGTACAAGA GGAAAAGAACAGGACATAAGACTGTAGAGGTCTCCAAGGATCAGCCAGATCATGCAAGCATATTTAGGCTATAAGAAGGTCTTTATTTTAAAAGCAAGGT TTTAAAGCTGTATAGTGACATAATGAGATTGTCCATTTTTCAGTATCGTCTTGGATGCTTTGTGAAGAGCAGGTGGAAAGGAGGCAATTGCCTAGTTCAT CGTAGAAGTAATGATGTCTTGGACTAGAATTAGGGTGAGTGAAAAAGAGAAGTGATCAAATTCCAGAAGTCTTTAGGACGAAGAATTGTGGAACTTGGTG TGGGGAGTGAGTAAGGGCAAAGATGAGGACCAGGGTTCTGGCTTGGGAACTTGGTTGTTTTTACTGAGATGGAGAAAACTGGAGGATGAGGCATTTTATT TTGGACATGTTAAAGTTAAGGTGACAATACAGCAAGGTGGAAATGTTGAGTGTGTTGTTGGTTATGCAGATTTGCAATTCAGGGGAGGAATCTGACTTGG AGACACAGATTTGAGAGCTATCAGCATATAGATAGCATATATATATCATATATATAGATGATACTTGAAGACGTGGTTGTGAAGGAGAACGTCTCAGGAG AGTGGAAGGCAAGAGGTCAGGGGACAGGAAGCCTGCACGACAGAAAAGGAGCAACCGCAAGTTGAAAAAGAAATAGGAAAATGTGTGAGTAAAGCTTACA GAAGAGAATGTTTTAATGAAGGGGAGGTAGTCAACGGCAGTACAGGCTACTGAGAGATCAAAGAACATTAATATTTTAAAGTAGCCATTGAATTTACAGT TGGGTTTTTTTTTTTTAAGTTAAGGGGTACATGTGCAGGTTTGTTGGATGGGTAAACTCAGGTCATGGAGGTTTGTTGTGCAGATCATTTCATCACCCAG GTATTAAGCCGTGGCACCCATTAGTTATTTTTCCTGATCCCTCCCTCTTCCTACCCGCCCCACTCTGGTAGGCCCCAGTGTCTGTTGCTCCCCTCAGTGT GCAGTTAGGATGTATTTGACCATCTGTGAGTGTTGTTTCAGAATAATGGGTGAAGAAATCAGACTACATTGGGTTCCTTAATTTAAGGCACTATGATGAA CACCTTCTGTGCTGGGTGCTAGAGTCCAGAATCTGACATAATCTCTGCTCTCGATACAGCAGATTTGTGGCAGATTGAAAAGTAGGCCGGGCGCGGTGGC TCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCTGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTGAAACCCTGTCTCTA CTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGTGCGCCTGTAATCCCAGCTACTCGGAGGCTGAGGCAGGAGAACTGCTTGAACCCGGGAAGCAGAGG CTGCAGTGAGCCAAGATCATGCCACTGCCCTCCAGCCTGGGCCACAGAGCAAGACTCTGTCTTAAAAAAGAAAAAGAAAACAAAAGTTGAGAGGTGAGCA AATAGTGAAAGTGAATGTACACCAGTTCTACAAGGTTGGCTGAGATAGGAAGGCAAGAAGAGCAGTAGTGATGGTGACTCAGAGTGGCAAAATCAAATTC TTTATTTTTTAAAATTGGTGGAGTTTAGTTTTTAGGTTTGACAGGTCAGGATCACCTGTCATCTTAGTCTAGACTTCACTGGTTCCCATTAGGTTCTACA CTATTTCTCTTTAATACTTTGCAAAGTCATTTGTTCCATTTTTCATATAATTCTCAAAGGGTACCCTGATAAAGACAGTAAGCACTGTTACTGTTTTGTT GGTGAGGAAATAGAGGCTTAGGCAGTTGCCCAGTGGGCCTAGCATCTAGGAAGACTCTAGCTGAGTCTAGGAAGACTCTAGGAAGATGCTGCCAGAAGGA AAAGGGGTGGAATTAATGAAACTGGAAGGTTGTGGTGCTGGTTTGAGGAGTAAAGTATGGGGGCCAAAGTTGGCTATATGCTGGATATGAAGAGGGGGTT AATTCCTTGCAGGTCTTCTTGAGATAGAAGTCCAGGCCCTGAGGTGGCAGGCAGCCTGATAGTGAACAGAACCCTTGTGCCCATACTTTTGCCCCCCGAG TTTGGCCTGCCCTGGCGCCACAGGCAGTACTGGAAGCTGATAAGTCAGGGCACAAATTAAAGGTCTAGGAGGCAAGACCAACTGATAAACAGGGGGCGTA ATGCTTCCTGGGGATAATCCCATCTCAAGGAAAAGCTTTTGGCTATTTCTGTGTTAGCAGATGCGAGCAAGTTGTGGAGGAAAATGATTAACTTAAAGGC CACACCCCCCAACCCCTTCAGCTGTACGGGCATCTGGATGGCCAGACCCCTGATCAGCTCCAGGCCTTGCCTAGAGTCGCACACAGGAAGTGACCACAAC ACTAAGGCATCCGGAGGCTGCTGGCCCAGACCACGGACCGCCCCCTGCTCCGCCCCTTGAATCTGCAGCCCCGAACTGGAGGGAATTTCCTTTTATGGCC CCAGGCTGTCAGGCAGGCCCAGCTCCAAACCAATTGTTTTGTTTTGTTTGTATACTTTACTTTATAAAATACATATTTCTTGGAAAAGAAAAAAATCAGG TATAGTTTTAATCCCAAAACAGCCATTCTCATTTTCTTCTGTTTTCTATGCACAGGATTGCTAAGTAGGGGAATCCTGCTTTGCATGGTGGTTTGTTATC TACCTCATCAGTAGTCAAAAGCATTTCTCCTTTTCAACATAAACCCTTCCAAAACATCATTTAAATTCCATTCCATTTTTAGATGTTAGTTTCAGTACCA GAGCCAAATAGTAAGTTTGTTCCCTCTTCTATAAGTTGTGGCCTGCCTTCTCCAAGACACCCTTCTCCCCCACTAACATGCACTGAACAGCTGCTGTCAA TCCATTCCCTCAAGAAACTTAAAGGTCTAACAGACAAGACAGTCCATTAACAGATTTTTACAATCCAGTGCAACAAGTGCTGAAGTAGGGGGACATATAA GATGTGGTGGAAAACAGAAAGGACCAATAGATGTTGCAGAAGGGGTGCAAGAGAAACCAGGGGAAGAGCACTACCACCTAAGGAAATGAGATGTGGGATA TGGTGAAGATAACACATTTTTTTATAGAGGTTGTTGAGATATCTAGGTGGTTGGTTTGGTTTGAAGCTCAGGGGAAGGTCTGAAATGGACAGCTTTGGGA GTTGCTGGCATGAATGAGAATCAAAGTCCTAGAGAGGTAAGCCTTCCCAGAGAGTATGTAATTATCAGTTTTTCAGGAATCAAAACCTTTGCCCCATCCC ACCTCCAGCTCAATGACCATTGCCCCTTCCTCAAAGGGACGATCATGGCTTCTCCCCCTTGCACTGGGCCTGCCGAGAGGGCCGCTCTGCTGTGGTTGAG ATGTTGATCATGCGGGGGGCACGGATCAATGTAATGAACCGTGGGGATGACACCCCCCTGCATCTGGCAGCCAGTCATGGACACCGTGATATTGTACAGA AGGTACGTACAAACTCCTTCGTCATCCACATCACATACATGCCATGAGGGTCAGTCACAGGCACTGTAACATACAGTAGAAAGCATGTGTGCTCTTCCCC CTTTTCCCATGCCCTGACACCAGTATCTCATTTGGAACTGACTGTACTTTCTGCCTCTTCTTTTTGTCTGGCCATGGGGTCCAGCTATTGCAGTACAAGG CAGACATCAATGCAGTGAATGAACACGGGAATGTGCCCCTGCACTATGCCTGTTTTTGGGGCCAAGATCAAGTGGCAGAGAGCGGGCAGAGAAGATGGGC CAGAATCTCAACCGTATTCCATACAAGGACACATTCTGGAAGGGGACCACCCGCACTCGGCCCCGTGAGTCACCACTGTGGGAAGAAGGGTTGTAAAAGG AAATAATCCTGGCCTCTTGGGGCTGGGTTAGGGTGAAGCTGGGTACCTGACCTGCCCACACTCTTAGGAAATGGAACCCTGAACAAACACTCTGGCATTG ACTTCAAACAGCTTAACTTCCTGACGAAGCTCAACGAGAATCACTCTGGAGAGCTATGGAAGGGCCGCTGGCAGGGCAATGACATTGTCGTGAAGGTGCT GAAGGTTCGAGACTGGAGTACAAGGAAGAGCAGGGACTTCAATGAAGAGTGTCCCCGGCTCAGGATTTTCTCGCATCCAAATGTGCTCCCAGTGCTAGGT GCCTGCCAGTCTCCACCTGCTCCTCATCCTACTCTCATCACACACTGGATGCCGTATGGATCCCTCTACAATGTACTACATGAAGGCACCAGTGAGTAGG GATGTTGAATTTCCTTGGGGAGGAAATGGCAGAGAGGGAGCCTCTCTGAACTATTTGACTTTTGCCTCCTCTCAGATTTCGTCGTGGACCAGAGCCAGGC TGTGAAGTTTGCTTTGGACATGGCAAGGGGCATGGCCTTCCTACACACACTAGAGCCCCTCATCCCACGACATGCACTCAATAGCCGTAGTGTAATGGTG AGGCCACAAGCTCACTCCTGGCCCAGGCCCCAAAAGCCCTTTGCCTATCTATGACTTACCTCCTTCTATCTGTTTTCTCTTCCTCAGATTGATGAGGACA TGACTGCCCGAATTAGCATGGCTGATGTCAAGTTCTCTTTCCAATGTCCTGGTCGCATGTATGCACCTGCCTGGGTAGCCCCCGAAGGTGAGTGAAGTCA TCATGTCGGGAGGTAAAAAAGGACCACCTCAGAAGTAGTGGAAGGGGGCAGAGACAGGACAGGCAAGGGGGCCAGAACAGACAAGCCCTATCTCTCCAGC TCTGCAGAAGAAGCCTGAAGACACAAACAGACGCTCAGCAGACATGTGGAGTTTTGCAGTGCTTCTGTGGGAACTGGTGACACGGGAGGTACCCTTTGCT GACCTCTCCAATATGGAGATTGGAATGAAGGTGAGAGCACAACAGCATACATTTGTGTTGCGGGAGTGGTTGGTGATGGTGAAAATAACTGTAGTGGGCC TTGGCTCCTCACATATTTGTTCGGATATACAGTAATCCTGTCCCAAGGGCCAGTGGCTTCTCTCTACATGACAGACTCAAATTGTGAGGCTGCTTTTTTT CTTGTATTCGCAGGTGGCATTGGAAGGCCTTCGGCCTACCATCCCACCAGGTATTTCCCCTCATGTGTGTAAGCTCATGAAGATCTGCATGAATGAAGAC CCTGCAAAGCGACCCAAATTTGACATGATTGTGCCTATCCTTGAGAAGATGCAGGACAAGTAGGACTGGAAGGTCCTTGCCTGAACTCCAGAGGTGTCGG GACATGGTTGGGGGAATGCACCTCCCCAAAGCAGCAGGCCTCTGGTTGCCTCCCCCGCCTCCAGTCATGGTACTACCCCAGCCATGGGGTCCATCCCCTT CCCCCATCCCTACCACTGTGGCCCCAAGAGGGGCGGGCTCAGAGCTTTGTCACTTGCCACATGGTGTCTCCCAACATGGGAGGGATCAGCCCCGCCTGTC ACAATAAAGTTTATTATGAAAAC

ILK.F11 (forward primer)
ILK.R12 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPSPGECERTRAPCHPSLLLSPK*GTEPTLGRT PGPLSQSIRSLNLPTSPNSVRG*GLVACSRTSVQ QTLPLRHPLPTLTRLYLASRGHSQGSSRSPELL HRPPLAGDAAQAPYPQHKHFSPVEDKAWGSSSF PGSLHSPQASPIQGTRRRDAAMDDIFTQCREGNA VAVRLWLDNTENDLNQG*AETVGG*EEG*RSPG YVEWSAHVWWWRLPLLASVMSAKHSLWGAEGFH RGRSLS*ILTAE*EFHRQRLQRAFQPQ*TAWPKA RGQGTEQREQEIVWSSIWLEDR*SLWVGALGTR GKEQDIRL*RSPRISQIMQAYLGYKKVFILKAR F*SCIVT**DCPFFSIVLDAL*RAGGKEAIA*FI VEVMMSWTRIRVSEKEK*SNSRSL*DEELWNLV WGVSKGKDEDQGSGLGTWLFLLRWRKLEDEAFY FGHVKVKVTIQQGGNVECVVGYADLQFRGGI*LG DTDLRAISI*IAYIYHIYR*YLKTWL*RRTSQE SGRQEVRGQEACTTEKEQPQVEKEIGKCVSKAY RRECFNEGEVVNGSTGY*EIKEH*YFKVAIEFTV GFFFFKLRGTCAGLLDG*TQVMEVCCADHFITQ VLSRGTH*LFFLIPPSSYPPHSGRPQCLLLPSV CS*DVFDHL*VLFQNNG*RNQTTLGSLI*GTMMN TFCAGC*SPESDIISALDTADLWQIEK*AGRGG SRL*SQHFGRPRLVDHEVRSSRPA*PTW*NPVS TKNTKISQAWWRAPVIPATRRLRQENCLNPGSRG CSEPRSCHCPPAWATEQDSVLKKKKKTKVER*A NSESECTPVLQGWLR*EGKKSSSDGDSEWQNQI LYFLKLVEFSF*V*QVRITCHLSLDFTGSH*VLH YFSLILCKVICSIFHIILKGYPDKDSKHCYCFV GEEIEA*AVAQWA*HLGRL*LSLGRL*EDAARR KRGGINETGRLWCWFEE*SMGAKVGYMLDMKRGL IPCRSS*DRSPGPEVAGSLIVNRTLVPILLPPE FGLPWRHRQYWKLISQGTN*RSRRQDQLINRGR NASWG*SHLKEKLLAISVLADASKLWRKMINLKA TPPNPFSCTGIWMARPLISSRPCLESHTGSDHN TKASGGCWPRPRTAPCSAP*ICSPELEGISFYG PRLSGRPSSKPIVLFCLYTLLYKIHISWKRKKSG IVLIPKQPFSFSSVFYAQDC*VGESCFAWWFVI YLISSQKHFSFST*TLPKHHLNSIPFLDVSFST RAK**VCSLFYKLWPAFSKTPFSPTNMH*TAAVN PFPQET*RSNRQDSPLTDFYNPVQQVLK*GDI* DVVENRKDQ*MLQKGCKRNQGKSTTT*GNEMWD MVKITHFFIEVVEISRWLVWFEAQGKV*NGQLWE LLA*MRIKVLER*AFPESM*LSVFQESKPLPHP TSSSMTIAPSSKGRSWLLPLALGLPRGPLCCG* DVDHAGGTDQCNEPWG*HPPASGSQSWTP*YCTE GTYKLLRHPHHIHAMRVSHRHCNIQ*KACVLFP LFPCPDTSISFGTDCTFCLFFLSGHGVQLLQYK ADINAVNEHGNVPLHYACFWGQDQVAESGQRRWA RISTVFHTRTHSGRGPPALGPVSHHCGKKGCKR K*SWPLGAGLG*SWVPDLPTLLGNGTLNKHSGI DFKQLNFLTKLNENHSGELWKGRWQGNDIVVKVL KVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLG ACQSPPAPHPTLITHWMPYGSLYNVLHEGTSE* GC*ISLGRKWQRGSLSELFDFCLLSDFVVDQSQA VKFALDMARGMAFLHTLEPLIPRHALNSRSVMV RPQAHSWPRPQKPFAYL*LTSFYLFSLPQIDED MTARISMADVKFSFQCPGRMYAPAWVAPEGE*SH HVGR*KRTTSEVVEGGRDRTGKGARTDKPYLSS SAEEA*RHKQTLSRHVEFCSASVGTGDTGGTLC *PLQYGDWNEGESTTAYICVAGVVGDGENNCSGP WLLTYLFGYTVILSQGPVASLYMTDSNCEAAFF LVFAGGIGRPSAYHPTRYFPSCV*AHEDLHE*R PCKATQI*HDCAYP*EDAGQVGLEGPCLNSRGVG TWLGECTSPKQQASGCLPRLQSWYYPSHGVHPL PPSLPLWPQEGRAQSFVTCHMVSPNMGGISPAC HNKVYYEN

Frame 1

ARVPVSARGPAPPVTPHFS*VPSKERSQPWGGP PVPSPRVYGA*TSPLPPTLFADRV*LPALGHPF SRHYLFVTPCPP*PAFTSRLEDTARDHPAAPNSF TDPH*PGTQLRPPTPNTNTSLL*RIKLGVHPPS LDHSTVLRLPQSRGLGAGTLLWTTFSLSAGRAT QSPFACGWTTRRTTSTRGELKRLVDERKARDLLA TWSGVLMSGGGGCLC*PV**VLSIACGGQRVFT EDAV*AES*LQNKSFTGRGYRELSSLSRLHGQR LGGKAQSRENRKLSGVPYGWRIDDLCGSEHWVQE EKNRT*DCRGLQGSARSCKHI*AIRRSLF*KQG FKAV**HNEIVHFSVSSWMLCEEQVERRQLPSS S*K**CLGLELG*VKKRSDQIPEVFRTKNCGTWC GE*VRAKMRTRVLAWELGCFY*DGENWRMRHFI LDMLKLR*QYSKVEMLSVLLVMQICNSGEESDL ETQI*ELSAYR*HIYIIYIDDT*RRGCEGERLRR VEGKRSGDRKPARQKRSNRKLKKK*ENV*VKLT EENVLMKGR*STAVQATERSKNINILK*PLNLQ LGFFFLS*GVHVQVCWMGKLRSWRFVVQIISSPR Y*AVAPISYFS*SLPLPTRPTLVGPSVCCSPQC AVRMYLTICECCFRIMGEEIRLHWVP*FKAL** TPSVLGARVQNLT*SLLSIQQICGRLKSRPGAVA HACNPSTLGGRGWWITRSGVRDQPDQHGETLSL LKIQKLARRGGVRL*SQLLGG*GRRTA*TREAE AAVSQDHATALQPGPQSKTLS*KRKRKQKLRGEQ IVKVNVHQFYKVG*DRKARRAVVMVTQSGKIKF FIF*NWWSLVFRFDRSGSPVILV*TSLVPIRFY TISL*YFAKSFVPFFI*FSKGTLIKTVSTVTVLL VRK*RLRQLPSGPSI*EDSS*V*EDSRKMLPEG KGVELMKLEGCGAGLRSKVWGPKLAICWI*RGG *FLAGLLEIEVQALRWQAA***TEPLCPYFCPPS LACPGATGSTGS**VRAQIKGLGGKTN**TGGV MLPGDNPISRKSFWLFLC*QMRASCGGK*LT*R PHPPTPSAVRASGWPDP*SAPGLA*SRTQEVTTT LRHPEAAGPDHGPPPAPPLESAAPNWREFPFMA PGCQAGPAPNQLFCFVCILYFIKYIFLGKEKNQ V*F*SQNSHSHFLLFSMHRIAK*GNPALHGGLLS TSSVVKSISPFQHKPFQNII*IPFHF*MLVSVP EPNSKFVPSSISCGLPSPRHPSPPLTCTEQLLS IHSLKKLKGLTDKTVH*QIFTIQCNKC*SRGTYK MWWKTERTNRCCRRGARETRGRALPPKEMRCGI W*R*HIFL*RLLRYLGGWFGLKLRGRSEMDSFG SCWHE*ESKS*RGKPSQRVCNYQFFRNQNLCPIP PPAQ*PLPLPQRDDHGFSPLHWACREGRSAVVE MLIMRGARINVMNRGDDTPLHLAASHGHRDIVQ KVRTNSFVIHITYMP*GSVTGTVTYSRKHVCSSP FSHALTPVSHLELTVLSASSFCLAMGSSYCSTR QTSMQ*MNTGMCPCTMPVFGAKIKWQRAGREDG PESQPYSIQGHILEGDHPHSAP*VTTVGRRVVKG NNPGLLGLG*GEAGYLTCPHS*EMEP*TNTLAL TSNSLTS*RSSTRITLESYGRAAGRAMTLS*RC *RFETGVQGRAGTSMKSVPGSGFSRIQMCSQC*V PASLHLLLILLSSHTGCRMDPSTMYYMKAPVSR DVEFPWGGNGREGASLNYLTFASSQISSWTRAR L*SLLWTWQGAWPSYTH*SPSSHDMHSIAVV*W* GHKLTPGPGPKSPLPIYDLPPSICFLFLRLMRT *LPELAWLMSSSLSNVLVACMHLPG*PPKVSEV IMSGGKKGPPQK*WKGAETGQARGPEQTSPISPA LQKKPEDTNRRSADMWSFAVLLWELVTREVPFA DLSNMEIGMKVRAQQHTFVLREWLVMVKITVVG LGSSHICSDIQ*SCPKGQWLLST*QTQIVRLLFF LYSQVALEGLRPTIPPGISPHVCKLMKICMNED PAKRPKFDMIVPILEKMQDK*DWKVLA*TPEVS GHGWGNAPPQSSRPLVASPASSHGTTPAMGSIPF PHPYHCGPKRGGLRALSLATWCLPTWEGSAPPV TIKFIMK

Frame 2

PESR*VREDPRPLSPLTSPESQVRNGANPGEDP RSPLPEYTEPEPPHFPQLCSRIGSSCLLSDIRS ADTTSSSPPAHPDPPLPRV*RTQPGIIPQPRTP SQTPTSRGRSSGPLPPTQTLLSCRG*SLGFILLP WITPQSSGFPNPGDSAPGRCYGRHFHSVPGGQR SRRSPVAGQHGERPQPGVS*NGWWMRGRLEISW LRGVECSCLVVAAAFASQCNEC*A*PVGGRGFSQ RTQFELNLDCRIRVSQAEATESFPASVDCMAKG *GARHRAERTGNCLEFHMAGG*MISVGRSIGYK RKRTGHKTVEVSKDQPDHASIFRL*EGLYFKSKV LKLYSDIMRLSIFQYRLGCFVKSRWKGGNCLVH RRSNDVLD*N*GE*KREVIKFQKSLGRRIVELG VGSE*GQR*GPGFWLGNLVVFTEMEKTGG*GILF WTC*S*GDNTARWKC*VCCWLCRFAIQGRNLTW RHRFESYQHIDSIYISYI*MILEDVVVKENVSG EWKARGQGTGSLHDRKGATAS*KRNRKMCE*SLQ KRMF**RGGSQRQYRLLRDQRTLIF*SSH*IYS WVFFF*VKGYMCRFVGWVNSGHGGLLCRSFHHP GIKPWHPLVIFPDPSLFLPAPLW*APVSVAPLSV QLGCI*PSVSVVSE*WVKKSDYIGFLNLRHYDE HLLCWVLESRI*HNLCSRYSRFVAD*KVGRARW LTPVIPALWEAEAGGSRGQEFETSLTNMVKPCLY *KYKN*PGVVACACNPSYSEAEAGELLEPGKQR LQ*AKIMPLPSSLGHRARLCLKKEKENKS*EVS K**K*MYTSSTRLAEIGRQEEQ**W*LRVAKSNS LFFKIGGV*FLGLTGQDHLSS*SRLHWFPLGST LFLFNTLQSHLFHFSYNSQRVP**RQ*ALLLFC W*GNRGLGSCPVGLASRKTLAESRKTLGRCCQKE KGWN**NWKVVVLV*GVKYGGQSWLYAGYEEGV NSLQVFLR*KSRP*GGRQPDSEQNPCAHTFAPR VWPALAPQAVLEADKSGHKLKV*EARPTDKQGA* CFLGIIPSQGKAFGYFCVSRCEQVVEEND*LKG HTPQPLQLYGHLDGQTPDQLQALPRVAHRK*PQ H*GIRRLLAQTTDRPLLRPLNLQPRTGGNFLLWP QAVRQAQLQTNCFVLFVYFTL*NTYFLEKKKIR YSFNPKTAILIFFCFLCTGLLSRGILLCMVVCY LPHQ*SKAFLLFNINPSKTSFKFHSIFRC*FQYQ SQIVSLFPLL*VVACLLQDTLLPH*HALNSCCQ SIPSRNLKV*QTRQSINRFLQSSATSAEVGGHI RCGGKQKGPIDVAEGVQEKPGEEHYHLRK*DVGY GEDNTFFYRGC*DI*VVGLV*SSGEGLKWTALG VAGMNENQSPREVSLPREYVIISFSGIKTFAPS HLQLNDHCPFLKGTIMASPPCTGPAERAALLWLR C*SCGGHGSM**TVGMTPPCIWQPVMDTVILYR RYVQTPSSSTSHTCHEGQSQAL*HTVESMCALP PFPMP*HQYLIWN*LYFLPLLFVWPWGPAIAVQG RHQCSE*TRECAPALCLFLGPRSSGRERAEKMG QNLNRIPYKDTFWKGTTRTRPRESPLWEEGL*K EIILASWGWVRVKLGT*PAHTLRKWNPEQTLWH* LQTA*LPDEAQRESLWRAMEGPLAGQ*HCREGA EGSRLEYKEEQGLQ*RVSPAQDFLASKCAPSAR CLPVSTCSSSYSHHTLDAVWIPLQCTT*RHQ*VG MLNFLGEEMAEREPL*TI*LLPPLRFRRGPEPG CEVCFGHGKGHGLPTHTRAPHPTTCTQ*P*CNG EATSSLLAQAPKALCLSMTYLLLSVFSSSD**GH DCPN*HG*CQVLFPMSWSHVCTCLGSPRR*VKS SCREVKKDHLRSSGRGQRQDRQGGQNRQALSLQ LCRRSLKTQTDAQQTCGVLQCFCGNW*HGRYPLL TSPIWRLE*R*EHNSIHLCCGSGW*W*K*L*WA LAPHIFVRIYSNPVPRASGFSLHDRLKL*GCFF SCIRRWHWKAFGLPSHQVFPLMCVSS*RSA*MKT LQSDPNLT*LCLSLRRCRTSRTGRSLPELQRCR DMVGGMHLPKAAGLWLPPPPPVMVLPQPWGPSP SPIPTTVAPRGAGSELCHLPHGVSQHGRDQPRLS Q*SLL*K

Sequence ILK.gAug05

ILK.F11 (forward primer)
ILK.R12 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPSPGECERTRAPCHPSLLLSPK*GTEPTLGRT PGPLSQSIRSLNLPTSPNSVRG*GLVACSRTSVQ QTLPLRHPLPTLTRLYLASRGHSQGSSRSPELL HRPPLAGDAAQAPYPQHKHFSPVEDKAWGSSSF PGSLHSPQASPIQGTRRRDAAMDDIFTQCREGNA VAVRLWLDNTENDLNQGDDHGFSPLHWACREGR SAVVEMLIMRGARINVMNRGDDTPLHLAASHGH RDIVQKLLQYKADINAVNEHGNVPLHYACFWGQD QVAEDLVANGALVSICNKYGEMPVDKAKAPLRE LLREKMGQNLNRIPYKDTFWKGTTRTRPRESPL WEEGL*KEIILASWGWVRVKLGT*PAHTLRKWNP EQTLWH*LQTA*LPDEAQRESLWRGDPCPSCPS LTKPP*ITCFVPVLSFSSS*WARKWQQHFKPPN PYLSCSYGRAAGRAMTLS*RC*RFETGVQGRAGT SMKSVPGSGFSRIQMCSQC*VPASLHLLLILLS SHTGCRMDPSTMYYMKAPISSWTRARL*SLLWT WQGAWPSYTH*SPSSHDMHSIAVV**LMRT*LPE LAWLMSSSLSNVLVACMHLPG*PPKLCRRSLKT QTDAQQTCGVLQCFCGNW*HGRYPLLTSPIWRL E*R*EHNSIHLCCGSGW*W*K*L*WALAPHIFVR IYSNPVPRASGFSLHDRLKL*GCFFSCIRRWHW KAFGLPSHQVFPLMCVSS*RSA*MKTLQSDPNL T*LCLSLRRCRTSRTGRSLPELQRCRDMVGGMHL PKAAGLWLPPPPPVMVLPQPWGPSPSPIPTTVA PRGAGSELCHLPHGVSQHGRDQPRLSQ*SLL*K

Frame 1

ARVPVSARGPAPPVTPHFS*VPSKERSQPWGGP PVPSPRVYGA*TSPLPPTLFADRV*LPALGHPF SRHYLFVTPCPP*PAFTSRLEDTARDHPAAPNSF TDPH*PGTQLRPPTPNTNTSLL*RIKLGVHPPS LDHSTVLRLPQSRGLGAGTLLWTTFSLSAGRAT QSPFACGWTTRRTTSTRGTIMASPPCTGPAERAA LLWLRC*SCGGHGSM**TVGMTPPCIWQPVMDT VILYRSYCSTRQTSMQ*MNTGMCPCTMPVFGAK IKWQRTWWQMGPLSASVTSMERCLWTKPRHP*ES FSERRWARISTVFHTRTHSGRGPPALGPVSHHC GKKGCKRK*SWPLGAGLG*SWVPDLPTLLGNGT LNKHSGIDFKQLNFLTKLNENHSGEVTPALLALP SLNPHKLLALYLF*VFPPVSGQGSGSNISSLLT PTCPAAMEGPLAGQ*HCREGAEGSRLEYKEEQG LQ*RVSPAQDFLASKCAPSARCLPVSTCSSSYSH HTLDAVWIPLQCTT*RHQFRRGPEPGCEVCFGH GKGHGLPTHTRAPHPTTCTQ*P*CND**GHDCP N*HG*CQVLFPMSWSHVCTCLGSPRSSAEEA*RH KQTLSRHVEFCSASVGTGDTGGTLC*PLQYGDW NEGESTTAYICVAGVVGDGENNCSGPWLLTYLF GYTVILSQGPVASLYMTDSNCEAAFFLVFAGGIG RPSAYHPTRYFPSCV*AHEDLHE*RPCKATQI* HDCAYP*EDAGQVGLEGPCLNSRGVGTWLGECT SPKQQASGCLPRLQSWYYPSHGVHPLPPSLPLWP QEGRAQSFVTCHMVSPNMGGISPACHNKVYYEN

Frame 2

PESR*VREDPRPLSPLTSPESQVRNGANPGEDP RSPLPEYTEPEPPHFPQLCSRIGSSCLLSDIRS ADTTSSSPPAHPDPPLPRV*RTQPGIIPQPRTP SQTPTSRGRSSGPLPPTQTLLSCRG*SLGFILLP WITPQSSGFPNPGDSAPGRCYGRHFHSVPGGQR SRRSPVAGQHGERPQPGGRSWLLPLALGLPRGP LCCG*DVDHAGGTDQCNEPWG*HPPASGSQSWTP *YCTEAIAVQGRHQCSE*TRECAPALCLFLGPR SSGRGPGGKWGPCQHL*QVWRDACGQSQGTPER ASPREDGPESQPYSIQGHILEGDHPHSAP*VTTV GRRVVKGNNPGLLGLG*GEAGYLTCPHS*EMEP *TNTLALTSNSLTS*RSSTRITLER*PLPFLPF PH*TPINYLLCTCFKFFLQLVGKEVAATFQAS*P LPVLQLWKGRWQGNDIVVKVLKVRDWSTRKSRD FNEECPRLRIFSHPNVLPVLGACQSPPAPHPTL ITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDM ARGMAFLHTLEPLIPRHALNSRSVMIDEDMTAR ISMADVKFSFQCPGRMYAPAWVAPEALQKKPED TNRRSADMWSFAVLLWELVTREVPFADLSNMEIG MKVRAQQHTFVLREWLVMVKITVVGLGSSHICS DIQ*SCPKGQWLLST*QTQIVRLLFFLYSQVAL EGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFD MIVPILEKMQDK*DWKVLA*TPEVSGHGWGNAP PQSSRPLVASPASSHGTTPAMGSIPFPHPYHCG PKRGGLRALSLATWCLPTWEGSAPPVTIKFIMK