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Sequences

Sequence ITGB1.cAug05

CAGTTTGATGTACTAAAGGCTTCTCGATTGGGAGACAGGAAACCTGGGTGTCAGTGCCGGGTCTGCCACTGAATTTCTGTGTGACCTTTGTCAGAGTACT
GTACTTCCAGAGCGTTGGTTTCCACATCTACCCTATAAAGCAGCCTGCATGTTTCTCTCATTCTCTCGTTCGAGGAGTTGGAGGATAGCTGCTGCCTGAT
ATGAATTTACAACCAATTTTCTGGATTGGACTGATCAGTTCAGTTTGCTGTGTGTTTGCTCAAACAGATGAAAATAGATGTTTAAAAGCAAATGCCAAAT
CATGTGGAGAATGTATACAAGCAGGGCCAAATTGTGGGTGGTGCACAAATTCAACATTTTTACAGGAAGGAATGCCTACTTCTGCACGATGTGATGATTT
AGAAGCCTTAAAAAAGAAGGGTTGCCCTCCAGATGACATAGAAAATCCCAGAGGCTCCAAAGATATAAAGAAAAATAAAAATGTAACCAACCGTAGCAAA
GGAACAGCAGAGAAGCTCAAGCCAGAGGATATTACTCAGATCCAACCACAGCAGTTGGTTTTGCGATTAAGATCAGGGGAGCCACAGACATTTACATTAA
AATTCAAGAGAGCTGAAGACTATCCCATTGACCTCTACTACCTTATGGACCTGTCTTACTCAATGAAAGACGATTTGGAGAATGTAAAAAGTCTTGGAAC
AGATCTGATGAATGAAATGAGGAGGATTACTTCGGACTTCAGAATTGGATTTGGCTCATTTGTGGAAAAGACTGTGATGCCTTACATTAGCACAACACCA
GCTAAGCTCAGGAACCCTTGCACAAGTGAACAGAACTGCACCAGCCCATTTAGCTACAAAAATGTGCTCAGTCTTACTAATAAAGGAGAAGTATTTAATG
AACTTGTTGGAAAACAGCGCATATCTGGAAATTTGGATTCTCCAGAAGGTGGTTTCGATGCCATCATGCAAGTTGCAGTTTGTGGATCACTGATTGGCTG
GAGGAATGTTACACGGCTGCTGGTGTTTTCCACAGATGCCGGGTTTCACTTTGCTGGAGATGGGAAACTTGGTGGCATTGTTTTACCAAATGATGGACAA
TGTCACCTGGAAAATAATATGTACACAATGAGCCATTATTATGATTATCCTTCTATTGCTCACCTTGTCCAGAAACTGAGTGAAAATAATATTCAGACAA
TTTTTGCAGTTACTGAAGAATTTCAGCCTGTTTACAAGGAGCTGAAAAACTTGATCCCTAAGTCAGCAGTAGGAACATTATCTGCAAATTCTAGCAATGT
AATTCAGTTGATCATTGATGCATACAATTCCCTTTCCTCAGAAGTCATTTTGGAAAACGGCAAATTGTCAGAAGGCGTAACAATAAGTTACAAATCTTAC
TGCAAGAACGGGGTGAATGGAACAGGGGAAAATGGAAGAAAATGTTCCAATATTTCCATTGGAGATGAGGTTCAATTTGAAATTAGCATAACTTCAAATA
AGTGTCCAAAAAAGGATTCTGACAGCTTTAAAATTAGGCCTCTGGGCTTTACGGAGGAAGTAGAGGTTATTCTTCAGTACATCTGTGAATGTGAATGCCA
AAGCGAAGGCATCCCTGAAAGTCCCAAGTGTCATGAAGGAAATGGGACATTTGAGTGTGGCGCGTGCAGGTGCAATGAAGGGCGTGTTGGTAGACATTGT
GAATGCAGCACAGATGAAGTTAACAGTGAAGACATGGATGCTTACTGCAGGAAAGAAAACAGTTCAGAAATCTGCAGTAACAATGGAGAGTGCGTCTGCG
GACAGTGTGTTTGTAGGAAGAGGGATAATACAAATGAAATTTATTCTGGCAAATTCTGCGAGTGTGATAATTTCAACTGTGATAGATCCAATGGCTTAAT
TTGTGGAGGAAATGGTGTTTGCAAGTGTCGTGTGTGTGAGTGCAACCCCAACTACACTGGCAGTGCATGTGACTGTTCTTTGGATACTAGTACTTGTGAA
GCCAGCAACGGACAGATCTGCAATGGCCGGGGCATCTGCGAGTGTGGTGTCTGTAAGTGTACAGATCCGAAGTTTCAAGGGCAAACGTGTGAGATGTGTC
AGACCTGCCTTGGTGTCTGTGCTGAGCATAAAGAATGTGTTCAGTGCAGAGCCTTCAATAAAGGAGAAAAGAAAGACACATGCACACAGGAATGTTCCTA
TTTTAACATTACCAAGGTAGAAAGTCGGGACAAATTACCCCAGCCGGTCCAACCTGATCCTGTGTCCCATTGTAAGGAGAAGGATGTTGACGACTGTTGG
TTCTATTTTACGTATTCAGTGAATGGGAACAACGAGGTCATGGTTCATGTTGTGGAGAATCCAGAGTGTCCCACTGGTCCAGACATCATTCCAATTGTAG
CTGGTGTGGTTGCTGGAATTGTTCTTATTGGCCTTGCATTACTGCTGATATGGAAGCTTTTAATGATAATTCATGACAGAAGGGAGTTTGCTAAATTTGA
AAAGGAGAAAATGAATGCCAAATGGGACACGTCTCTCTCTGTCGCCCAGCCTGGAGTGCAGTGGTGTGATATCAGCTCACTGCAACCTCTGACTTCCAGA
TTCCAGCAATTCTCCTGCCTCAGCCTCCCGAGTACCTGGGATTACAGGGTGAAAATCCTATTTATAAGAGTGCCGTAACAACTGTGGTCAATCCGAAGTA
TGAGGGAAAATGAGTACTGCCCGTGCAAATCCCACAACACTGAATGCAAAGTAGCAATTTCCATAGTCACAGTTAGGTAGCTTTAGGGCAATATTGCCAT
GGTTTTACTCATGTGCAGGTTTTGAAAATGTACAATATGTATAATTTTTAAAATGTTTTATTATTTTGAAAATAATGTTGTAATTCATGCCAGGGACTGA
CAAAAGACTTGAGACAGGATGGTTACTCTTGTCAGCTAAGGTCACATTGTGCCTTTTTGACCTTTTCTTCCTGGACTATTGAAATCAAGCTTATTGGATT
AAGTGATATTTCTATAGCGATTGAAAGGGCAATAGTTAAAGTAATGAGCATGATGAGAGTTTCTGTTAATCATGTATTAAAACTGATTTTTAGCTTTACA
AATATGTCAGTTTGCAGTTATGCAGAATCCAAAGTAAATGTCCTGCTAGCTAGTTAAGGATTGTTTTAAATCTGTTATTTTGCTATTTGCCTGTTAGACA
TGACTGATGACATATCTGAAAGACAAGTATGTTGAGAGTTGCTGGTGTAAAATACGTTTGAAATAGTTGATCTACAAAGGCCATGGGAAAAATTCAGAGA
GTTAGGAAGGAAAAACCAATAGCTTTAAAACCTGTGTGCCATTTTAAGAGTTACTTAATGTTTGGTAACTTTTATGCCTTCACTTTACAAATTCAAGCCT
TAGATAAAAGAACCGAGCAATTTTCTGCTAAAAAGTCCTTGATTTAGCACTATTTACATACAGGCCATACTTTACAAAGTATTTGCTGAATGGGGACCTT
TTGAGTTGAATTTATTTTATTATTTTTATTTTGTTTAATGTCTGGTGCTTTCTGTCACCTCTTCTAATCTTTTAATGTATTTGTTTGCAATTTTGGGGTA
AGACTTTTTTTATGAGTACTTTTTCTTTGAAGTTTTAGCGGTCAATTTGCCTTTTTAATGAACATGTGAAGTTATACTGTGGCTATGCAACAGCTCTCAC
CTACGCGAGTCTTACTTTGAGTTAGTGCCATAACAGACCACTGTATGTTTACTTCTCACCATTTGAGTTGCCCATCTTGTTTCACACTAGTCACATTCTT
GTTTTAAGTGCCTTTAGTTTTAACAGTTCACTTTTTACAGTGCTATTTACTGAAGTTATTTATTAAATATGCCTAAAATACTTAAATCGGATGTCTTGAC
TCTGATGTATTTTATCAGGTTGTGTGCATGAAATTTTTATAGATTAAAGAAGTTGAGGAAAAGCA
  • ITGB1.F2 (forward primer)
  • ITGB1.R15 (reverse primer)
  • Intron retention

Frame 0

QFDVLKASRLGDRKPGCQCRVCH*ISV*PLSEY
CTSRALVSTSTL*SSLHVSLILSFEELEDSCCLI
*IYNQFSGLD*SVQFAVCLLKQMKIDV*KQMPN
HVENVYKQGQIVGGAQIQHFYRKECLLLHDVMI
*KP*KRRVALQMT*KIPEAPKI*RKIKM*PTVAK
EQQRSSSQRILLRSNHSSWFCD*DQGSHRHLH*
NSRELKTIPLTSTTLWTCLTQ*KTIWRM*KVLE
QI**MK*GGLLRTSELDLAHLWKRL*CLTLAQHQ
LSSGTLAQVNRTAPAHLATKMCSVLLIKEKYLM
NLLENSAYLEIWILQKVVSMPSCKLQFVDH*LA
GGMLHGCWCFPQMPGFTLLEMGNLVALFYQMMDN
VTWKIICTQ*AIIMIILLLLTLSRN*VKIIFRQ
FLQLLKNFSLFTRS*KT*SLSQQ*EHYLQILAM
*FS*SLMHTIPFPQKSFWKTANCQKA*Q*VTNLT
ARTG*MEQGKMEENVPIFPLEMRFNLKLA*LQI
SVQKRILTALKLGLWALRRK*RLFFSTSVNVNA
KAKASLKVPSVMKEMGHLSVARAGAMKGVLVDIV
NAAQMKLTVKTWMLTAGKKTVQKSAVTMESASA
DSVFVGRGIIQMKFILANSASVIISTVIDPMA*
FVEEMVFASVVCVSATPTTLAVHVTVLWILVLVK
PATDRSAMAGASASVVSVSVQIRSFKGKRVRCV
RPALVSVLSIKNVFSAEPSIKEKRKTHAHRNVP
ILTLPR*KVGTNYPSRSNLILCPIVRRRMLTTVG
SILRIQ*MGTTRSWFMLWRIQSVPLVQTSFQL*
LVWLLELFLLALHYC*YGSF***FMTEGSLLNL
KRRK*MPNGTRLSLSPSLECSGVISAHCNL*LPD
SSNSPASASRVPGITG*KSYL*ECRNNCGQSEV
*GKMSTARANPTTLNAK*QFP*SQLGSFRAILP
WFYSCAGFENVQYV*FLKCFIILKIML*FMPGTD
KRLETGWLLLSAKVTLCLFDLFFLDY*NQAYWI
K*YFYSD*KGNS*SNEHDESFC*SCIKTDF*LY
KYVSLQLCRIQSKCPAS*LRIVLNLLFCYLPVRH
D**HI*KTSMLRVAGVKYV*NS*STKAMGKIQR
VRKEKPIALKPVCHFKSYLMFGNFYAFTLQIQA
LDKRTEQFSAKKSLI*HYLHTGHTLQSIC*MGTF
*VEFILLFLFCLMSGAFCHLF*SFNVFVCNFGV
RLFL*VLFL*SFSGQFAFLMNM*SYTVAMQQLS
PTRVLL*VSAITDHCMFTSHHLSCPSCFTLVTFL
F*VPLVLTVHFLQCYLLKLFIKYA*NT*IGCLD
SDVFYQVVCMKFL*IKEVEEK

Frame 1

SLMY*RLLDWETGNLGVSAGSATEFLCDLCQST
VLPERWFPHLPYKAACMFLSFSRSRSWRIAAA*
YEFTTNFLDWTDQFSLLCVCSNR*K*MFKSKCQI
MWRMYTSRAKLWVVHKFNIFTGRNAYFCTM**F
RSLKKEGLPSR*HRKSQRLQRYKEK*KCNQP*Q
RNSREAQARGYYSDPTTAVGFAIKIRGATDIYIK
IQES*RLSH*PLLPYGPVLLNERRFGECKKSWN
RSDE*NEEDYFGLQNWIWLICGKDCDALH*HNT
S*AQEPLHK*TELHQPI*LQKCAQSY**RRSI**
TCWKTAHIWKFGFSRRWFRCHHASCSLWITDWL
EECYTAAGVFHRCRVSLCWRWETWWHCFTK*WT
MSPGK*YVHNEPLL*LSFYCSPCPETE*K*YSDN
FCSY*RISACLQGAEKLDP*VSSRNIICKF*QC
NSVDH*CIQFPFLRSHFGKRQIVRRRNNKLQIL
LQERGEWNRGKWKKMFQYFHWR*GSI*N*HNFK*
VSKKGF*QL*N*ASGLYGGSRGYSSVHL*M*MP
KRRHP*KSQVS*RKWDI*VWRVQVQ*RACW*TL
*MQHR*S*Q*RHGCLLQERKQFRNLQ*QWRVRLR
TVCL*EEG*YK*NLFWQILRV**FQL**IQWLN
LWRKWCLQVSCV*VQPQLHWQCM*LFFGY*YL*
SQQRTDLQWPGHLRVWCL*VYRSEVSRANV*DVS
DLPWCLC*A*RMCSVQSLQ*RRKERHMHTGMFL
F*HYQGRKSGQITPAGPT*SCVPL*GEGC*RLL
VLFYVFSEWEQRGHGSCCGESRVSHWSRHHSNCS
WCGCWNCSYWPCITADMEAFNDNS*QKGVC*I*
KGENECQMGHVSLCRPAWSAVV*YQLTATSDFQ
IPAILLPQPPEYLGLQGENPIYKSAVTTVVNPKY
EGK*VLPVQIPQH*MQSSNFHSHS*VALGQYCH
GFTHVQVLKMYNMYNF*NVLLF*K*CCNSCQGL
TKDLRQDGYSCQLRSHCAFLTFSSWTIEIKLIGL
SDISIAIERAIVKVMSMMRVSVNHVLKLIFSFT
NMSVCSYAESKVNVLLAS*GLF*ICYFAICLLD
MTDDISERQVC*ELLV*NTFEIVDLQRPWEKFRE
LGRKNQ*L*NLCAILRVT*CLVTFMPSLYKFKP
*IKEPSNFLLKSP*FSTIYIQAILYKVFAEWGP
FELNLFYYFYFV*CLVLSVTSSNLLMYLFAILG*
DFFYEYFFFEVLAVNLPF**TCEVILWLCNSSH
LRESYFELVP*QTTVCLLLTI*VAHLVSH*SHS
CFKCL*F*QFTFYSAIY*SYLLNMPKILKSDVLT
LMYFIRLCA*NFYRLKKLRKS

Frame 2

V*CTKGFSIGRQETWVSVPGLPLNFCVTFVRVL
YFQSVGFHIYPIKQPACFSHSLVRGVGG*LLPD
MNLQPIFWIGLISSVCCVFAQTDENRCLKANAK
SCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDL
EALKKKGCPPDDIENPRGSKDIKKNKNVTNRSK
GTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTL
KFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGT
DLMNEMRRITSDFRIGFGSFVEKTVMPYISTTP
AKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFN
ELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGW
RNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQ
CHLENNMYTMSHYYDYPSIAHLVQKLSENNIQT
IFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNV
IQLIIDAYNSLSSEVILENGKLSEGVTISYKSY
CKNGVNGTGENGRKCSNISIGDEVQFEISITSN
KCPKKDSDSFKIRPLGFTEEVEVILQYICECECQ
SEGIPESPKCHEGNGTFECGACRCNEGRVGRHC
ECSTDEVNSEDMDAYCRKENSSEICSNNGECVC
GQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLI
CGGNGVCKCRVCECNPNYTGSACDCSLDTSTCE
ASNGQICNGRGICECGVCKCTDPKFQGQTCEMC
QTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSY
FNITKVESRDKLPQPVQPDPVSHCKEKDVDDCW
FYFTYSVNGNNEVMVHVVENPECPTGPDIIPIV
AGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFE
KEKMNAKWDTSLSVAQPGVQWCDISSLQPLTSR
FQQFSCLSLPSTWDYRVKILFIRVP*QLWSIRS
MRENEYCPCKSHNTECKVAISIVTVR*L*GNIAM
VLLMCRF*KCTICIIFKMFYYFENNVVIHARD*
QKT*DRMVTLVS*GHIVPF*PFLPGLLKSSLLD
*VIFL*RLKGQ*LK**A**EFLLIMY*N*FLALQ
ICQFAVMQNPK*MSC*LVKDCFKSVILLFAC*T
*LMTYLKDKYVESCWCKIRLK*LIYKGHGKNSE
S*EGKTNSFKTCVPF*ELLNVW*LLCLHFTNSSL
R*KNRAIFC*KVLDLALFTYRPYFTKYLLNGDL
LS*IYFIIFILFNVWCFLSPLLIF*CICLQFWG
KTFFMSTFSLKF*RSICLFNEHVKLYCGYATALT
YASLTLS*CHNRPLYVYFSPFELPILFHTSHIL
VLSAFSFNSSLFTVLFTEVIY*ICLKYLNRMS*
L*CILSGCVHEIFID*RS*GKA

Sequence ITGB1.fAug05

ATGAATTTACAACCAATTTTCTGGATTGGACTGATCAGTTCAGTTTGCTGTGTGTTTGCTCAAACAGATGAAAATAGATGTTTAAAAGCAAATGCCAAAT
CATGTGGAGAATGTATACAAGCAGGGCCAAATTGTGGGTGGTGCACAAATTCAACATTTTTACAGGAAGGAATGCCTACTTCTGCACGATGTGATGATTT
AGAAGCCTTAAAAAAGAAGGGTTGCCCTCCAGATGACATAGAAAATCCCAGAGGCTCCAAAGATATAAAGAAAAATAAAAATGTAACCAACCGTAGCAAA
GGAACAGCAGAGAAGCTCAAGCCAGAGGATATTACTCAGATCCAACCACAGCAGTTGGTTTTGCGATTAAGATCAGGGGAGCCACAGACATTTACATTAA
AATTCAAGAGAGCTGAAGACTATCCCATTGACCTCTACTACCTTATGGACCTGTCTTACTCAATGAAAGACGATTTGGAGAATGTAAAAAGTCTTGGAAC
AGATCTGATGAATGAAATGAGGAGGATTACTTCGGACTTCAGAATTGGATTTGGCTCATTTGTGGAAAAGACTGTGATGCCTTACATTAGCACAACACCA
GCTAAGCTCAGGAACCCTTGCACAAGTGAACAGAACTGCACCAGCCCATTTAGCTACAAAAATGTGCTCAGTCTTACTAATAAAGGAGAAGTATTTAATG
AACTTGTTGGAAAACAGCGCATATCTGGAAATTTGGATTCTCCAGAAGGTGGTTTCGATGCCATCATGCAAGTTGCAGTTTGTGGATCACTGATTGGCTG
GAGGAATGTTACACGGCTGCTGGTGTTTTCCACAGATGCCGGGTTTCACTTTGCTGGAGATGGGAAACTTGGTGGCATTGTTTTACCAAATGATGGACAA
TGTCACCTGGAAAATAATATGTACACAATGAGCCATTATTATGATTATCCTTCTATTGCTCACCTTGTCCAGAAACTGAGTGAAAATAATATTCAGACAA
TTTTTGCAGTTACTGAAGAATTTCAGCCTGTTTACAAGGAGCTGAAAAACTTGATCCCTAAGTCAGCAGTAGGAACATTATCTGCAAATTCTAGCAATGT
AATTCAGTTGATCATTGATGCATACAATTCCCTTTCCTCAGAAGTCATTTTGGAAAACGGCAAATTGTCAGAAGGCGTAACAATAAGTTACAAATCTTAC
TGCAAGAACGGGGTGAATGGAACAGGGGAAAATGGAAGAAAATGTTCCAATATTTCCATTGGAGATGAGGTTCAATTTGAAATTAGCATAACTTCAAATA
AGTGTCCAAAAAAGGATTCTGACAGCTTTAAAATTAGGCCTCTGGGCTTTACGGAGGAAGTAGAGGTTATTCTTCAGTACATCTGTGAATGTGAATGCCA
AAGCGAAGGCATCCCTGAAAGTCCCAAGTGTCATGAAGGAAATGGGACATTTGAGTGTGGCGCGTGCAGGTGCAATGAAGGGCGTGTTGGTAGACATTGT
GAATGCAGCACAGATGAAGTTAACAGTGAAGACATGGATGCTTACTGCAGGAAAGAAAACAGTTCAGAAATCTGCAGTAACAATGGAGAGTGCGTCTGCG
GACAGTGTGTTTGTAGGAAGAGGGATAATACAAATGAAATTTATTCTGGCAAATTCTGCGAGTGTGATAATTTCAACTGTGATAGATCCAATGGCTTAAT
TTGTGGAGGAAATGGTGTTTGCAAGTGTCGTGTGTGTGAGTGCAACCCCAACTACACTGGCAGTGCATGTGACTGTTCTTTGGATACTAGTACTTGTGAA
GCCAGCAACGGACAGATCTGCAATGGCCGGGGCATCTGCGAGTGTGGTGTCTGTAAGTGTACAGATCCGAAGTTTCAAGGGCAAACGTGTGAGATGTGTC
AGACCTGCCTTGGTGTCTGTGCTGAGCATAAAGAATGTGTTCAGTGCAGAGCCTTCAATAAAGGAGAAAAGAAAGACACATGCACACAGGAATGTTCCTA
TTTTAACATTACCAAGGTAGAAAGTCGGGACAAATTACCCCAGCCGGTCCAACCTGATCCTGTGTCCCATTGTAAGGAGAAGGATGTTGACGACTGTTGG
TTCTATTTTACGTATTCAGTGAATGGGAACAACGAGGTCATGGTTCATGTTGTGGAGAATCCAGAGTGTCCCACTGGTCCAGACATCATTCCAATTGTAG
CTGGTGTGGTTGCTGGAATTGTTCTTATTGGCCTTGCATTACTGCTGATATGGAAGCTTTTAATGATAATTCATGACAGAAGGGAGTTTGCTAAATTTGA
AAAGGAGAAAATGAATGCCAAATGGGACACGGTAAGTTACAAAACATCCAAAAAGCAAAGTGGCTTATAAAGTAAATGTAATACTCCTAAGACTTATGTA
TTAGCTGTCAGGCTGATTATTAAAGTCCTTTCTAAGTATTTTATTCCCCCAAAAGTTTCTTACTCAAGGAATTTGCATTTAGTGAAAAACAGAAAGCATC
CTAAATATATCCCATTGAAACAAAACATTGATTATAAGCATGTATATTCTGGTTACTGTGGCCAGTATTTTTATTTCTTTAATAGTTTTGATCCTAAATC
TGCCTTTTCATCTAATGTGAAGTAGAATCCTAAATAATGTTATCTGTGTAGCAAGCTATTCAATGGAAAGCTGCTTCTTTCTTTAAAACAAACAAACAAA
AAAAACCTTCAGTGAAAGCCAGATTCAAAAGGTTATATACCAAGCTTGTCCAACTCGCAGCTCGTCGGCCAGGACATGCAGCCCAGAATAGCTTTGAATG
TGGCCCCAACACAAATTTGTAAACTTTCTTAGAAATTGTAATTATTATTATTATTTTTTTTTGGTAACTTTTTTTTAAAGCTCATCAGCTATCGTTAGTG
TATTTTATGTGTGGCCCAAGACAATTCTTCTTCTTCCAGTGTGGCCCAGGGAAGCCAAAAGATTGGACACCCCTGCTATATACTATATGATTCCATTTAG
AGGACATTCTGGAAAAGCAAAACTGTAGGGGCAAAAATCAGTGGTTGCTAGGGGCTGGAATGGGGGAAAGTGTTGACCACAGAGGGGCGTAAGGGATCTT
CCTTGGGATGATCTTGATTGTGGGTGGATTTATGTATTTGAAAACTCACAGAACTATGTACTTTAAAAAGATGTATGTTCCTCTATGAAAATTATATCTC
AGTAAACTTTGGCTTATAAAAATCTTAAAAGCCCTAAGTGACCGAAAGGTTATGTTAGCATTGAGTGCTTTGAAATATGGAGTCAGAGGGTGGGGTAACC
AAATGTTGGCCTTTGTGTATTCATCTTTTGATACAAGAAAGCAATGCCAATCTTCAGTATTTTTAAATTGTAAATGAATTTTGTAGTTAGTTGCACTTTA
CTACAATTTGCTTGGGTTTTTAAAAATTATCAATTTTTTAAATTACAAATTTCAGAAGGGTAGAAATAAAAAAAAAGACATTTATAGTGTTCAAACACTA
TAAAGGGATTTTTTTTTTCTGATGAAATCATTAGAAACTATTTCTTTTACTTTAGCAAGAAAATCCGATTTACAAGAGTCCTATTAATAATTTCAAGAAT
CCAAACTACGGACGTAAAGCTGGTCTCTAAATTGCCGTTCGTATTTTGTTTCACTTTCTGCCTTTGCATGTGAACTTCTATCTTTTTTACTGCACTGTGT
GTCCTTTATCACTATTGCTGTTCTTTTTTTAAGTCTGGCTTTAAATATCGCTATTGTGGCTTTTCTTTTTGTATGTTAACTTCGCGTTTAGAAAGACCGT
AAAATAAGTAATGTGATATAGAAACAGCAGCCATGTTGTAGTGAAAAGAGAATGTGCTTTGCAATCAGTTAGACCTCAGGTCAAATCCCACCTCTTCCAC
TTAGTAAGTAGAACAGCATTGAGTCACTTCACTTCTCTCAACTGTCACAGGGAGTCAGTACTCTGTTTTGTATGGGGTGGTTGTATTTGGCAAATGCACT
CCAGTATATGAAATCAGCCTGTCAGCTCTAATCCTCATAACAGTCCTGTGCCTAGAATGATATTATCCTATTATCTTTTGTTCTTTTTCTGTTTATTTAT
TTATTTATTTTGAGACAGTCTCTCTCTGTCGCCCAGCCTGGAGTGCAGTGGTGTGATATCAGCTCACTGCAACCTCTGACTTCCAGATTCCAGCAATTCT
CCTGCCTCAGCCTCCCGAGTACCTGGGATTACAGGTGCCTGCCACCATGCTAGGCTAATTTTTTTGTTTTTAGTAGAGATGGGGTTTCACCACATTGGCT
GGGCTGGCCTTGA
  • ITGB1.F2 (forward primer)
  • ITGB1.R15 (reverse primer)
  • Intron retention

Frame 0

MNLQPIFWIGLISSVCCVFAQTDENRCLKANAK
SCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDL
EALKKKGCPPDDIENPRGSKDIKKNKNVTNRSK
GTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTL
KFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGT
DLMNEMRRITSDFRIGFGSFVEKTVMPYISTTP
AKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFN
ELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGW
RNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQ
CHLENNMYTMSHYYDYPSIAHLVQKLSENNIQT
IFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNV
IQLIIDAYNSLSSEVILENGKLSEGVTISYKSY
CKNGVNGTGENGRKCSNISIGDEVQFEISITSN
KCPKKDSDSFKIRPLGFTEEVEVILQYICECECQ
SEGIPESPKCHEGNGTFECGACRCNEGRVGRHC
ECSTDEVNSEDMDAYCRKENSSEICSNNGECVC
GQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLI
CGGNGVCKCRVCECNPNYTGSACDCSLDTSTCE
ASNGQICNGRGICECGVCKCTDPKFQGQTCEMC
QTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSY
FNITKVESRDKLPQPVQPDPVSHCKEKDVDDCW
FYFTYSVNGNNEVMVHVVENPECPTGPDIIPIV
AGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFE
KEKMNAKWDTVSYKTSKKQSGL*SKCNTPKTYV
LAVRLIIKVLSKYFIPPKVSYSRNLHLVKNRKH
PKYIPLKQNIDYKHVYSGYCGQYFYFFNSFDPKS
AFSSNVK*NPK*CYLCSKLFNGKLLLSLKQTNK
KNLQ*KPDSKGYIPSLSNSQLVGQDMQPRIALN
VAPTQICKLS*KL*LLLLFFFGNFFLKLISYR*C
ILCVAQDNSSSSSVAQGSQKIGHPCYILYDSI*
RTFWKSKTVGAKISGC*GLEWGKVLTTEGRKGS
SLG*S*LWVDLCI*KLTELCTLKRCMFLYENYIS
VNFGL*KS*KP*VTERLC*H*VL*NMESEGGVT
KCWPLCIHLLIQESNANLQYF*IVNEFCS*LHF
TTICLGF*KLSIF*ITNFRRVEIKKKTFIVFKHY
KGIFFF**NH*KLFLLL*QENPIYKSPINNFKN
PNYGRKAGL*IAVRILFHFLPLHVNFYLFYCTV
CPLSLLLFFF*VWL*ISLLWLFFLYVNFAFRKTV
K*VM*YRNSSHVVVKRECALQSVRPQVKSHLFH
LVSRTALSHFTSLNCHRESVLCFVWGGCIWQMH
SSI*NQPVSSNPHNSPVPRMILSYYLLFFFCLFI
YLF*DSLSLSPSLECSGVISAHCNL*LPDSSNS
PASASRVPGITGACHHARLIFLFLVEMGFHHIG
WAGL

Frame 1

*IYNQFSGLD*SVQFAVCLLKQMKIDV*KQMPN
HVENVYKQGQIVGGAQIQHFYRKECLLLHDVMI
*KP*KRRVALQMT*KIPEAPKI*RKIKM*PTVAK
EQQRSSSQRILLRSNHSSWFCD*DQGSHRHLH*
NSRELKTIPLTSTTLWTCLTQ*KTIWRM*KVLE
QI**MK*GGLLRTSELDLAHLWKRL*CLTLAQHQ
LSSGTLAQVNRTAPAHLATKMCSVLLIKEKYLM
NLLENSAYLEIWILQKVVSMPSCKLQFVDH*LA
GGMLHGCWCFPQMPGFTLLEMGNLVALFYQMMDN
VTWKIICTQ*AIIMIILLLLTLSRN*VKIIFRQ
FLQLLKNFSLFTRS*KT*SLSQQ*EHYLQILAM
*FS*SLMHTIPFPQKSFWKTANCQKA*Q*VTNLT
ARTG*MEQGKMEENVPIFPLEMRFNLKLA*LQI
SVQKRILTALKLGLWALRRK*RLFFSTSVNVNA
KAKASLKVPSVMKEMGHLSVARAGAMKGVLVDIV
NAAQMKLTVKTWMLTAGKKTVQKSAVTMESASA
DSVFVGRGIIQMKFILANSASVIISTVIDPMA*
FVEEMVFASVVCVSATPTTLAVHVTVLWILVLVK
PATDRSAMAGASASVVSVSVQIRSFKGKRVRCV
RPALVSVLSIKNVFSAEPSIKEKRKTHAHRNVP
ILTLPR*KVGTNYPSRSNLILCPIVRRRMLTTVG
SILRIQ*MGTTRSWFMLWRIQSVPLVQTSFQL*
LVWLLELFLLALHYC*YGSF***FMTEGSLLNL
KRRK*MPNGTR*VTKHPKSKVAYKVNVILLRLMY
*LSG*LLKSFLSILFPQKFLTQGICI**KTESI
LNISH*NKTLIISMYILVTVASIFISLIVLILN
LPFHLM*SRILNNVICVASYSMESCFFL*NKQTK
KTFSESQIQKVIYQACPTRSSSARTCSPE*L*M
WPQHKFVNFLRNCNYYYYFFLVTFF*SSSAIVS
VFYVWPKTILLLPVWPREAKRLDTPAIYYMIPFR
GHSGKAKL*GQKSVVARGWNGGKC*PQRGVRDL
PWDDLDCGWIYVFENSQNYVL*KDVCSSMKIIS
Q*TLAYKNLKSPK*PKGYVSIECFEIWSQRVG*P
NVGLCVFIF*YKKAMPIFSIFKL*MNFVVSCTL
LQFAWVFKNYQFFKLQISEG*K*KKRHL*CSNT
IKGFFFSDEIIRNYFFYFSKKIRFTRVLLIISRI
QTTDVKLVSKLPFVFCFTFCLCM*TSIFFTALC
VLYHYCCSFFKSGFKYRYCGFSFCMLTSRLERP
*NK*CDIETAAML**KENVLCNQLDLRSNPTSST
**VEQH*VTSLLSTVTGSQYSVLYGVVVFGKCT
PVYEISLSALILITVLCLE*YYPIIFCSFSVYL
FIYFETVSLCRPAWSAVV*YQLTATSDFQIPAIL
LPQPPEYLGLQVPATMLG*FFCF**RWGFTTLA
GLAL

Frame 2

EFTTNFLDWTDQFSLLCVCSNR*K*MFKSKCQI
MWRMYTSRAKLWVVHKFNIFTGRNAYFCTM**F
RSLKKEGLPSR*HRKSQRLQRYKEK*KCNQP*Q
RNSREAQARGYYSDPTTAVGFAIKIRGATDIYIK
IQES*RLSH*PLLPYGPVLLNERRFGECKKSWN
RSDE*NEEDYFGLQNWIWLICGKDCDALH*HNT
S*AQEPLHK*TELHQPI*LQKCAQSY**RRSI**
TCWKTAHIWKFGFSRRWFRCHHASCSLWITDWL
EECYTAAGVFHRCRVSLCWRWETWWHCFTK*WT
MSPGK*YVHNEPLL*LSFYCSPCPETE*K*YSDN
FCSY*RISACLQGAEKLDP*VSSRNIICKF*QC
NSVDH*CIQFPFLRSHFGKRQIVRRRNNKLQIL
LQERGEWNRGKWKKMFQYFHWR*GSI*N*HNFK*
VSKKGF*QL*N*ASGLYGGSRGYSSVHL*M*MP
KRRHP*KSQVS*RKWDI*VWRVQVQ*RACW*TL
*MQHR*S*Q*RHGCLLQERKQFRNLQ*QWRVRLR
TVCL*EEG*YK*NLFWQILRV**FQL**IQWLN
LWRKWCLQVSCV*VQPQLHWQCM*LFFGY*YL*
SQQRTDLQWPGHLRVWCL*VYRSEVSRANV*DVS
DLPWCLC*A*RMCSVQSLQ*RRKERHMHTGMFL
F*HYQGRKSGQITPAGPT*SCVPL*GEGC*RLL
VLFYVFSEWEQRGHGSCCGESRVSHWSRHHSNCS
WCGCWNCSYWPCITADMEAFNDNS*QKGVC*I*
KGENECQMGHGKLQNIQKAKWLIK*M*YS*DLC
ISCQADY*SPF*VFYSPKSFLLKEFAFSEKQKAS
*IYPIETKH*L*ACIFWLLWPVFLFL**F*S*I
CLFI*CEVES*IMLSV*QAIQWKAASFFKTNKQ
KKPSVKARFKRLYTKLVQLAARRPGHAAQNSFEC
GPNTNL*TFLEIVIIIIIFFW*LFFKAHQLSLV
YFMCGPRQFFFFQCGPGKPKDWTPLLYTI*FHL
EDILEKQNCRGKNQWLLGAGMGESVDHRGA*GIF
LGMILIVGGFMYLKTHRTMYFKKMYVPL*KLYL
SKLWLIKILKALSDRKVMLALSALKYGVRGWGN
QMLAFVYSSFDTRKQCQSSVFLNCK*IL*LVALY
YNLLGFLKIINFLNYKFQKGRNKKKDIYSVQTL
*RDFFFLMKSLETISFTLARKSDLQESY**FQE
SKLRT*SWSLNCRSYFVSLSAFACELLSFLLHCV
SFITIAVLFLSLALNIAIVAFLFVC*LRV*KDR
KISNVI*KQQPCCSEKRMCFAIS*TSGQIPPLP
LSK*NSIESLHFSQLSQGVSTLFCMGWLYLANAL
QYMKSACQL*SS*QSCA*NDIILLSFVLFLFIY
LFILRQSLSVAQPGVQWCDISSLQPLTSRFQQF
SCLSLPSTWDYRCLPPC*ANFFVFSRDGVSPHWL
GWP*

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