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Sequences

Sequence EPHA2.bAug05

EPHA2.u.f.1 (forward primer)
EPHA2.u.r.2 (reverse primer)
Single exon skipping

Frame 0

GSHPTSIKDSGQEGQKLRAGRRAGADTEAGVQA CGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGA VRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCN VMSGDQDNWLRTNWVYRGEAERIFIELKFTVRD CNSFPGGASSCKETFNLYYAESDLDYGTNFQKRL FTKIDTIAPDEITVSSDFEARHVKLNVEERSVG PLTRKGFYLAFQDIGACVALLSVRVYYKKCPEL LQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPP GGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVED ACQGE*RFWVAGKGVLHSQVLVFSFGIMESW*A HPCFDTH*FC*WKL*ILPRHQFPY*GWTNSFIKN E*IVAGHGGSCL*SQHFGRLRWVDHLSPGV*DQ PGQHNETPSLPKTQKLAGCGGMHL*SQLLGRLK HENQLLEPGRWRMQ*AEIAPLYSSLG*QRRCLS

Frame 1

VLTQLPLRTRGRRGRSCAQAGGRERTPRPACRR AGVREPGSGGSDREREARHGAPGSPRLLRPAVG LCAGRGRGGAGQGSGT*CRTS*MTCRSTCTPCAT *CLATRTTGSAPTGCTEERLSVSSLSSSLLYVT ATASLVAPAPARRLSTSTMPSRTWTTAPTSRSA CSPRLTPLRPMRSPSAATSRHAT*S*TWRSAPWG RSPAKASTWPSRISVPVWRCSPSVSTTRSAPSC CRAWPTSLRPSPALMHLPWPLWPAPVWTMPWCH RGVKSPVCTVQWMASGWCPLGSACARQATRRWRM PARVSDGSGLQGRGFCIPRFWSLALESWNLGKL IPALTHISSVSGNYRSFPDISFLIKAGLTHSLR MNELWLGTVAHACNPSTLGG*GGWIT*AQEFETS LGNIMKRRLYQKHKN*LGVVACTCNPSYLGG*S TRINCLNPGGGGCSKLRSHHCTPAWGDRGDACL

Frame 2

FSPNFH*GLGAGGAEVARRPAGGSGHRGRRAGV RVCGSRARGDRTESEKRGMELQAARACFALLWG CALAAAAAAQGKEVGPDAEHHE*HADLHVLRVQ RDVWRPGQLAPHQLGVPRRG*AYLH*AQVYCT*L QQLPWWRQLLQGDFQPLLCRVGPGLRHQLPEAP VHQD*HHCAR*DHRQQRLRGTPREAERGGALRG AAHPQRLLPGLPGYRCLCGAALRPCLLQEVPRAA AGPGPLP*DHRRL*CTFPGHCGRHLCGPCRGAT GG*RAPYALCSGWRVAGAHWAVPVPGRLREGGG CLPG*VTVLGCREGGSAFPGSGL*LWNHGILVSS SLL*HTLVLLVEIIDPSQTSVSLLRLD*LIH*E *MNCGWARWLMPVIPALWEAEVGGSLEPRSLRP AWAT**NAVSTKNTKISWVWWHAPVIPATWEAEA RESIA*TREVEDAVS*DRTTVLQPGVTEEMLV*

Sequence EPHA2.aAug05

GGTTCTCACCCAACTTCCATTAAGGACTCGGGGCAGGAGGGGCAGAAGTTGCGCGCAGGCCGGCGGGCGGGAGCGGACACCGAGGCCGGCGTGCAGGCGT GCGGGTGTGCGGGAGCCGGGCTCGGGGGGATCGGACCGAGAGCGAGAAGCGCGGCATGGAGCTCCAGGCAGCCCGCGCCTGCTTCGCCCTGCTGTGGGGC TGTGCGCTGGCCGCGGCCGCGGCGGCGCAGGGCAAGGAAGTGGTACTGCTGGACTTTGCTGCAGCTGGAGGGGAGCTCGGCTGGCTCACACACCCGTATG GCAAAGGGTGGGACCTGATGCAGAACATCATGAATGACATGCCGATCTACATGTACTCCGTGTGCAACGTGATGTCTGGCGACCAGGACAACTGGCTCCG CACCAACTGGGTGTACCGAGGAGAGGCTGAGCGTATCTTCATTGAGCTCAAGTTTACTGTACGTGACTGCAACAGCTTCCCTGGTGGCGCCAGCTCCTGC AAGGAGACTTTCAACCTCTACTATGCCGAGTCGGACCTGGACTACGGCACCAACTTCCAGAAGCGCCTGTTCACCAAGATTGACACCATTGCGCCCGATG AGATCACCGTCAGCAGCGACTTCGAGGCACGCCACGTGAAGCTGAACGTGGAGGAGCGCTCCGTGGGGCCGCTCACCCGCAAAGGCTTCTACCTGGCCTT CCAGGATATCGGTGCCTGTGTGGCGCTGCTCTCCGTCCGTGTCTACTACAAGAAGTGCCCCGAGCTGCTGCAGGGCCTGGCCCACTTCCCTGAGACCATC GCCGGCTCTGATGCACCTTCCCTGGCCACTGTGGCCGGCACCTGTGTGGACCATGCCGTGGTGCCACCGGGGGGTGAAGAGCCCCGTATGCACTGTGCAG TGGATGGCGAGTGGCTGGTGCCCATTGGGCAGTGCCTGTGCCAGGCAGGCTACGAGAAGGTGGAGGATGCCTGCCAGGCCTGCTCGCCTGGATTTTTTAA GTTTGAGGCATCTGAGAGCCCCTGCTTGGAGTGCCCTGAGCACACGCTGCCATCCCCTGAGGGTGCCACCTCCTGCGAGTGTGAGGAAGGCTTCTTCCGG GCACCTCAGGACCCAGCGTCGATGCCTTGCACACGACCCCCCTCCGCCCCACACTACCTCACAGCCGTGGGCATGGGTGCCAAGGTGGAGCTGCGCTGGA CGCCCCCTCAGGACAGCGGGGGCCGCGAGGACATTGTCTACAGCGTCACCTGCGAACAGTGCTGGCCCGAGTCTGGGGAATGCGGGCCGTGTGAGGCCAG TGTGCGCTACTCGGAGCCTCCTCACGGACTGACCCGCACCAGTGTGACAGTGAGCGACCTGGAGCCCCACATGAACTACACCTTCACCGTGGAGGCCCGC AATGGCGTCTCAGGCCTGGTAACCAGCCGCAGCTTCCGTACTGCCAGTGTCAGCATCAACCAGACAGAGCCCCCCAAGGTGAGGCTGGAGGGCCGCAGCA CCACCTCGCTTAGCGTCTCCTGGAGCATCCCCCCGCCGCAGCAGAGCCGAGTGTGGAAGTACGAGGTCACTTACCGCAAGAAGGGAGACTCCAACAGCTA CAATGTGCGCCGCACCGAGGGTTTCTCCGTGACCCTGGACGACCTGGCCCCAGACACCACCTACCTGGTCCAGGTGCAGGCACTGACGCAGGAGGGCCAG GGGGCCGGCAGCAAGGTGCACGAATTCCAGACGCTGTCCCCGGAGGGATCTGGCAACTTGGCGGTGATTGGCGGCGTGGCTGTCGGTGTGGTCCTGCTTC TGGTGCTGGCAGGAGTTGGCTTCTTTATCCACCGCAGGAGGAAGAACCAGCGTGCCCGCCAGTCCCCGGAGGACGTTTACTTCTCCAAGTCAGAACAACT GAAGCCCCTGAAGACATACGTGGACCCCCACACATATGAGGACCCCAACCAGGCTGTGTTGAAGTTCACTACCGAGATCCATCCATCCTGTGTCACTCGG CAGAAGGTGATCGGAGCAGGAGAGTTTGGGGAGGTGTACAAGGGCATGCTGAAGACATCCTCGGGGAAGAAGGAGGTGCCGGTGGCCATCAAGACGCTGA AAGCCGGCTACACAGAGAAGCAGCGAGTGGACTTCCTCGGCGAGGCCGGCATCATGGGCCAGTTCAGCCACCACAACATCATCCGCCTAGAGGGCGTCAT CTCCAAATACAAGCCCATGATGATCATCACTGAGTACATGGAGAATGGGGCCCTGGACAAGTTCCTTCGGGAGAAGGATGGCGAGTTCAGCGTGCTGCAG CTGGTGGGCATGCTGCGGGGCATCGCAGCTGGCATGAAGTACCTGGCCAACATGAACTATGTGCACCGTGACCTGGCTGCCCGCAACATCCTCGTCAACA GCAACCTGGTCTGCAAGGTGTCTGACTTTGGCCTGTCCCGCGTGCTGGAGGACGACCCCGAGGCCACCTACACCACCAGTGGCGGCAAGATCCCCATCCG CTGGACCGCCCCGGAGGCCATTTCCTACCGGAAGTTCACCTCTGCCAGCGACGTGTGGAGCTTTGGCATTGTCATGTGGGAGGTGATGACCTATGGCGAG CGGCCCTACTGGGAGTTGTCCAACCACGAGGTGATGAAAGCCATCAATGATGGCTTCCGGCTCCCCACACCCATGGACTGCCCCTCCGCCATCTACCAGC TCATGATGCAGTGCTGGCAGCAGGAGCGTGCCCGCCGCCCCAAGTTCGCTGACATCGTCAGCATCCTGGACAAGCTCATTCGTGCCCCTGACTCCCTCAA GACCCTGGCTGACTTTGACCCCCGCGTGTCTATCCGGCTCCCCAGCACGAGCGGCTCGGAGGGGGTGCCCTTCCGCACGGTGTCCGAGTGGCTGGAGTCC ATCAAGATGCAGCAGTATACGGAGCACTTCATGGCGGCCGGCTACACTGCCATCGAGAAGGTGGTGCAGATGACCAACGACGACATCAAGAGGATTGGGG TGCGGCTGCCCGGCCACCAGAAGCGCATCGCCTACAGCCTGCTGGGACTCAAGGACCAGGTGAACACTGTGGGGATCCCCATCTGAGCCTCGACAGGGCC TGGAGCCCCATCGGCCAAGAATACTTGAAGAAACAGAGTGGCCTCCCTGCTGTGCCATGCTGGGCCACTGGGGACTTTATTTATTTCTAGTTCTTTCCTC CCCCTGCAACTTCCGCTGAGGGGTCTCGGATGACACCCTGGCCTGAACTGAGGAGATGACCAGGGATGCTGGGCTGGGCCCTCTTTCCCTGCGAGACGCA CACAGCTGAGCACTTAGCAGGCACCGCCACGTCCCAGCATCCCTGGAGCAGGAGCCCCGCCACAGCCTTCGGACAGACATATGGGATATTCCCAAGCCGA CCTTCCCTCCGCCTTCTCCCACATGAGGCCATCTCAGGAGATGGAGGGCTTGGCCCAGCGCCAAGTAAACAGGGTACCTCAAGCCCCATTTCCTCACACT AAGAGGGCAGACTGTGAACTTGACTGGGTGAGACCCAAAGCGGTCCCTGTCCCTCTAGTGCCTTCTTTAGACCCTCGGGCCCCATCCTCATCCCTGACTG GCCAAACCCTTGCTTTCCTGGGCCTTTGCAAGATGCTTGGTTGTGTTGAGGTTTTTAAATATATATTTTGTACTTTGTGGAGAGAATGTGTGTGTGTGGC AGGGGGCCCCGCCAGGGCTGGGGACAGAGGGTGTCAAACATTCGTGAGCTGGGGACTCAGGGACCGGTGCTGCAGGAGTGTCCTGCCCATGCCCCAGTCG GCCCCATCTCTCATCCTTTTGGATAAGTTTCTATTCTGTCAGTGTTAAAGATTTTGTTTTGTTGGACATTTTTTTCGAATCTTAATTTATTATTTTTTTT ATATTTATTGTTAGAAAATGACTTATTTCTGCTCTGGAATAAAGTTGCAGATGATTCAAACCGATC

EPHA2.u.f.1 (forward primer)
EPHA2.u.r.2 (reverse primer)
Single exon skipping

Frame 0

GSHPTSIKDSGQEGQKLRAGRRAGADTEAGVQA CGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGA VRWPRPRRRRARKWYCWTLLQLEGSSAGSHTRM AKGGT*CRTS*MTCRSTCTPCAT*CLATRTTGS APTGCTEERLSVSSLSSSLLYVTATASLVAPAPA RRLSTSTMPSRTWTTAPTSRSACSPRLTPLRPM RSPSAATSRHAT*S*TWRSAPWGRSPAKASTWP SRISVPVWRCSPSVSTTRSAPSCCRAWPTSLRPS PALMHLPWPLWPAPVWTMPWCHRGVKSPVCTVQ WMASGWCPLGSACARQATRRWRMPARPARLDFL SLRHLRAPAWSALSTRCHPLRVPPPASVRKASSG HLRTQRRCLAHDPPPPHTTSQPWAWVPRWSCAG RPLRTAGAARTLSTASPANSAGPSLGNAGRVRP VCATRSLLTD*PAPV*Q*ATWSPT*TTPSPWRPA MASQAW*PAAASVLPVSASTRQSPPR*GWRAAA PPRLASPGASPRRSRAECGSTRSLTARRETPTA TMCAAPRVSP*PWTTWPQTPPTWSRCRH*RRRAR GPAARCTNSRRCPRRDLATWR*LAAWLSVWSCF WCWQELASLSTAGGRTSVPASPRRTFTSPSQNN *SP*RHTWTPTHMRTPTRLC*SSLPRSIHPVSLG RR*SEQESLGRCTRAC*RHPRGRRRCRWPSRR* KPATQRSSEWTSSARPASWASSATTTSSA*RAS SPNTSP**SSLSTWRMGPWTSSFGRRMASSACCS WWACCGASQLA*STWPT*TMCTVTWLPATSSST ATWSARCLTLACPACWRTTPRPPTPPVAARSPS AGPPRRPFPTGSSPLPATCGALALSCGR**PMAS GPTGSCPTTR**KPSMMASGSPHPWTAPPPSTS S*CSAGSRSVPAAPSSLTSSASWTSSFVPLTPS RPWLTLTPACLSGSPARAARRGCPSARCPSGWSP SRCSSIRSTSWRPATLPSRRWCR*PTTTSRGLG CGCPATRSASPTACWDSRTR*TLWGSPSEPRQG LEPHRPRILEETEWPPCCAMLGHWGLYLFLVLSS PCNFR*GVSDDTLA*TEEMTRDAGLGPLSLRDA HS*ALSRHRHVPASLEQEPRHSLRTDIWDIPKP TFPPPSPT*GHLRRWRAWPSAK*TGYLKPHFLTL RGQTVNLTG*DPKRSLSL*CLL*TLGPHPHP*L AKPLLSWAFARCLVVLRFLNIYFVLCGENVCVW QGAPPGLGTEGVKHS*AGDSGTGAAGVSCPCPSR PHLSSFWISFYSVSVKDFVLLDIFFES*FIIFF IFIVRK*LISALE*SCR*FKPI

Frame 1

VLTQLPLRTRGRRGRSCAQAGGRERTPRPACRR AGVREPGSGGSDREREARHGAPGSPRLLRPAVG LCAGRGRGGAGQGSGTAGLCCSWRGARLAHTPVW QRVGPDAEHHE*HADLHVLRVQRDVWRPGQLAP HQLGVPRRG*AYLH*AQVYCT*LQQLPWWRQLL QGDFQPLLCRVGPGLRHQLPEAPVHQD*HHCAR* DHRQQRLRGTPREAERGGALRGAAHPQRLLPGL PGYRCLCGAALRPCLLQEVPRAAAGPGPLP*DH RRL*CTFPGHCGRHLCGPCRGATGG*RAPYALCS GWRVAGAHWAVPVPGRLREGGGCLPGLLAWIF* V*GI*EPLLGVP*AHAAIP*GCHLLRV*GRLLP GTSGPSVDALHTTPLRPTLPHSRGHGCQGGAALD APSGQRGPRGHCLQRHLRTVLARVWGMRAV*GQ CALLGASSRTDPHQCDSERPGAPHELHLHRGGP QWRLRPGNQPQLPYCQCQHQPDRAPQGEAGGPQH HLA*RLLEHPPAAAEPSVEVRGHLPQEGRLQQL QCAPHRGFLRDPGRPGPRHHLPGPGAGTDAGGP GGRQQGARIPDAVPGGIWQLGGDWRRGCRCGPAS GAGRSWLLYPPQEEEPACPPVPGGRLLLQVRTT EAPEDIRGPPHI*GPQPGCVEVHYRDPSILCHS AEGDRSRRVWGGVQGHAEDILGEEGGAGGHQDAE SRLHREAASGLPRRGRHHGPVQPPQHHPPRGRH LQIQAHDDHH*VHGEWGPGQVPSGEGWRVQRAA AGGHAAGHRSWHEVPGQHELCAP*PGCPQHPRQQ QPGLQGV*LWPVPRAGGRPRGHLHHQWRQDPHP LDRPGGHFLPEVHLCQRRVELWHCHVGGDDLWR AALLGVVQPRGDESHQ*WLPAPHTHGLPLRHLPA HDAVLAAGACPPPQVR*HRQHPGQAHSCP*LPQ DPG*L*PPRVYPAPQHERLGGGALPHGVRVAGV HQDAAVYGALHGGRLHCHREGGADDQRRHQEDWG AAARPPEAHRLQPAGTQGPGEHCGDPHLSLDRA WSPIGQEYLKKQSGLPAVPCWATGDFIYF*FFP PPATSAEGSRMTPWPELRR*PGMLGWALFPCETH TAEHLAGTATSQHPWSRSPATAFGQTYGIFPSR PSLRLLPHEAISGDGGLGPAPSKQGTSSPISSH *EGRL*T*LGETQSGPCPSSAFFRPSGPILIPDW PNPCFPGPLQDAWLC*GF*IYILYFVERMCVCG RGPRQGWGQRVSNIRELGTQGPVLQECPAHAPV GPISHPFG*VSILSVLKILFCWTFFSNLNLLFFL YLLLENDLFLLWNKVADDSNR

Frame 2

FSPNFH*GLGAGGAEVARRPAGGSGHRGRRAGV RVCGSRARGDRTESEKRGMELQAARACFALLWG CALAAAAAAQGKEVVLLDFAAAGGELGWLTHPY GKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLR TNWVYRGEAERIFIELKFTVRDCNSFPGGASSC KETFNLYYAESDLDYGTNFQKRLFTKIDTIAPD EITVSSDFEARHVKLNVEERSVGPLTRKGFYLAF QDIGACVALLSVRVYYKKCPELLQGLAHFPETI AGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCA VDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFK FEASESPCLECPEHTLPSPEGATSCECEEGFFR APQDPASMPCTRPPSAPHYLTAVGMGAKVELRW TPPQDSGGREDIVYSVTCEQCWPESGECGPCEAS VRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEAR NGVSGLVTSRSFRTASVSINQTEPPKVRLEGRS TTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSY NVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQ GAGSKVHEFQTLSPEGSGNLAVIGGVAVGVVLL LVLAGVGFFIHRRRKNQRARQSPEDVYFSKSEQL KPLKTYVDPHTYEDPNQAVLKFTTEIHPSCVTR QKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTL KAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVI SKYKPMMIITEYMENGALDKFLREKDGEFSVLQ LVGMLRGIAAGMKYLANMNYVHRDLAARNILVN SNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIR WTAPEAISYRKFTSASDVWSFGIVMWEVMTYGE RPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQ LMMQCWQQERARRPKFADIVSILDKLIRAPDSLK TLADFDPRVSIRLPSTSGSEGVPFRTVSEWLES IKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIG VRLPGHQKRIAYSLLGLKDQVNTVGIPI*ASTGP GAPSAKNT*RNRVASLLCHAGPLGTLFISSSFL PLQLPLRGLG*HPGLN*GDDQGCWAGPSFPARR TQLST*QAPPRPSIPGAGAPPQPSDRHMGYSQAD LPSAFSHMRPSQEMEGLAQRQVNRVPQAPFPHT KRADCELDWVRPKAVPVPLVPSLDPRAPSSSLT GQTLAFLGLCKMLGCVEVFKYIFCTLWRECVCVA GGPARAGDRGCQTFVSWGLRDRCCRSVLPMPQS APSLILLDKFLFCQC*RFCFVGHFFRILIYYFF YIYC*KMTYFCSGIKLQMIQTD