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Sequences

Sequence FGFR2.pAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGA GGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGGCCGCCGG TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGG ATATCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCA TAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCA CAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACA CGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACAC TGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGT GAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAAT CTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCG GGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTA CTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGA GATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATC AGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCT GAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACG TTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTT ACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAA CGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGC TTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTC CCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTC AAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGC TGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACC TAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTG CTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCT ATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGG AAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAG GTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTA ATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAG CCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACA CAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATA CCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAA TGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTA ATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQALGDK*FQCRSAGSVQCDR GGCWGIYM*GLQLYRAGQPVCLAHCPAKTARPP VLTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLG YPFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA *GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLC TS*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QH ASLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*H WASPWEKVALGKWSWRKQWELTKTSPRRRSPWP *RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*I FLEPAHRMGLSMS*LSMPLKATSENTSEPGGHP GWSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWS TWLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPE ISTI*TITKRPPMGGFQSSGWLQKPCLIEYTLI RVMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC *RKDTEWISQPTAPTNCT***GTVGMQCPPRDQR SSSW*KTWIEFSLSQPMRNTWTSANLSNSIHLV TLTQEVLVLQEMILFFLQTPCLTNHAFLSIHT* TAVLKHE*LCLPVPKQDSTGNLATLSRETMPPRA CCLHLYIWIRGVNNWKSNQHMCKDLYS*KLVIF PRRRRRFLEQWTATSHHVTPLTCRAYWLWTSRT QGGRAFCLPC*FCNNWRRFMSAHTYRAQMQYIGA GCM*IYSNYV*IYIIYLQGVIFCIDFKWMSQCT *KIGLSFFNSYLLNAVLTHNFLIFTEQRWKNTF AFRENGITLIY**IGNIQNN*SFIVFFVI*VAFL CRQHSRLVNLLLGLN*LSDPLKREYLQYMTNLG KMKF*FICV*MLLSDDCS*TS*MPHIKRTHS*E GVSFWCATLSLRQRNV*LDFPR*MVPASS*KMP* SIP*GQTLVEMIAECASLWQLAFCF*VAH*SD* PVFSSVNFDNGFQTLWRWRRLLGSSSPIIEN*N TELFC**FWGYVHLFKGLLSSNSGRTSPKDPASY LHQTKYRRCSFCTKVLCFALETPTHFAIAVQDE CRLH*SYVLQNWRKYLIKPVNFYTDNKNVSTDI NVNKTK*MSRNLFF*

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTE ADAGEYICKVSNYIGQANQSAWLTVLPKQQGRR C*HHGQRD*GSLYSECNF*GRWGIYVLGG*FYW DILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLH RGLLNRLYGGNSHPVPNEEHDQEARLQQPAGCA QADQTYPPAETGNSFG*VQLLHELQHPAGEDNN TPLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADT GQAPGRRLLWASGHGGSSGN*QRQAQGGGHRGR EDVER*CHRERPF*SGVRDGDDEDDWETQEYHK SSWSLHTGWASLCHS*VCL*RQPPRIPPSPEATR DGVLL*H*PCS*GADDLQGLGVMHLPAGQRHGV LGFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQ RYQQYRLLQKDHQWAASSQVDGSRSPV**SIHSS E*CLVLRGVNVGDLHFRGLALPRDSRGGTF*AA EGRTQNG*ASQLHQRTVHDDEGLLACSALPETN VQAVGRRLGSNSHSHNQ*GILGPQPTSRTVFT*L P*HKKFLFFRR*FCFFSRPHALRTMPSSVSTHK RQC*NMNDCVCLSPNRTALGT*LH*AGRPCLPE LVVSTCIYGSEE*IIGKVISICVKIYTVENL*SS PGGEEGFWSSGLPQATM*PLSPAVRTGCGPVGL KVDVRSAFLVNFVIIGEDLCQHTLTEHKCSI*V LDVCKYIQIMYKYILYIYKELFFVLILNGCPNAP RKLVSLFLIAIC*MLFLHIIS*FSPSRGGKILL LSGKMV*R*FINKLVIYKTINHL*FFL*FKWHF YAGSTAD*LIYCLDLTSYQIL*KENIYNI*LIWG K*SFDLFVFKCCCQTIVLRPPKCPILKELIHRK VFHFGVQPCHYVNATSNWTSQDKWYQRPLKRCL NPFLEDRP*LK**QNVLLSGSWPSASELHINQIS LYSLQ*ILIMASRLFGVGDAC*DLQVPS*KIET QSCSADSFGDTSIFLRDCFHLILAGPHQKIQPH TYIRQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMN ADYTDLMCYKIGESI**NLLIFILTIKMFLQIL MLTRQNKCHATYFF

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSSTRG*IVPMQKCWLCSM*P RRMLGNIYVRSPII*GRPTSLPGSLSCQNSKAAG VNTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIG ISFHSAWLTVLPAPGREKEITASPDYLEIAIYC IGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVH KLTKRIPLRRQVTVSAESSSSMNSNTPLVRITT RLSSTADTPMLAGVSEYELPEDPKWEFPRDKLT LGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAV KMLKDDATEKDLSDLVSEMEMMKMIGKHKNIIN LLGACTQDGPLYVIVEYASKGNLREYLRARRPP GMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEY LASQKCIHRDLAARNVLVTENNVMKIADFGLAR DINNIDYYKKTTNGRLPVKWMAPEALFDRVYTH QSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL KEGHRMDKPANCTNELYMMMRDCWHAVPSQRPT FKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPS YPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHIN GSVKT*MTVSACPQTGQHWEPSYTEQGDHASQS LLSPLVYMDQRSK*LEK*SAYV*RFIQLKTCNL PQEEKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DS RWTCVLPSLLIL**LEKIYVSTHLQSTNAVYRC WMYVNIFKLCINIYYIFTRSYFLY*F*MDVPMH LENWSLFF**LFAKCCSYT*FLNFHRAEVEKYFC FQGKWYNVNLLINW*YTKQLIIYSFFCNLSGIS MQAAQQTS*SIAWT*LVIRSFEKRIFTIYD*FG ENEVLIYLCLNAAVRRLFLDLLNAPY*KNSFIGR CFILVCNPVITSTQRLTGLPKINGTSVLLKDAL IHSLRTDLS*NDSRMCFSLAAGLLLLSCTLIRL ACILFSEF**WLPDSLALETPVRIFKSHHRKLKH RVVLLIVLGIRPSF*GIAFI*FWQDLTKRSSLI PTSDKISPLFLLY*SIVFCFGNTHSLCNSRAR* MQITLILCVTKLEKVFNKTC*FLY*Q*KCFYRY* C*QDKINVTQLIFL

Sequence FGFR2.lAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGT AACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATATCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGA GAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCC GAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTC CAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAA CTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAG TGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGA GATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCC TCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCA AGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGT AACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTC AAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGG GCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACAT GATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAG GAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAG ACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCA CTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAA TCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTA ACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGAT TTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACA AGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACA CATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAA ACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATC AGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCT TAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGG ACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCT CTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGG AGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGC TTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTT TGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAA CCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQGRRC*HHGQRD*GSLYSEC NF*GRWGIYVLGG*FYWDILSLCMVDSSASAWK RKGDYSFPRLPGDSHLLHRGLLNRLYGGNSHPVP NEEHDQEARLQQPAGCAQADQTYPPAETGFG*V QLLHELQHPAGEDNNTPLFNGRHPHAGRGLRV* TSRGPKMGVSKR*ADTGQAPGRRLLWASGHGGSS GN*QRQAQGGGHRGREDVER*CHRERPF*SGVR DGDDEDDWETQEYHKSSWSLHTGWASLCHS*VC L*RQPPRIPPSPEATRDGVLL*H*PCS*GADDLQ GLGVMHLPAGQRHGVLGFPKMYSSRFSSQKCFG NRKQCDENSRLWTRQRYQQYRLLQKDHQWAASS QVDGSRSPV**SIHSSE*CLVLRGVNVGDLHFRG LALPRDSRGGTF*AAEGRTQNG*ASQLHQRTVH DDEGLLACSALPETNVQAVGRRLGSNSHSHNQ* GILGPQPTSRTVFT*LP*HKKFLFFRR*FCFFSR PHALRTMPSSVSTHKRQC*NMNDCVCLSPNRTA LGT*LH*AGRPCLPELVVSTCIYGSEE*IIGKV ISICVKIYTVENL*SSPGGEEGFWSSGLPQATM* PLSPAVRTGCGPVGLKVDVRSAFLVNFVIIGED LCQHTLTEHKCSI*VLDVCKYIQIMYKYILYIY KELFFVLILNGCPNAPRKLVSLFLIAIC*MLFLH IIS*FSPSRGGKILLLSGKMV*R*FINKLVIYK TINHL*FFL*FKWHFYAGSTAD*LIYCLDLTSY QIL*KENIYNI*LIWGK*SFDLFVFKCCCQTIVL RPPKCPILKELIHRKVFHFGVQPCHYVNATSNW TSQDKWYQRPLKRCLNPFLEDRP*LK**QNVLL SGSWPSASELHINQISLYSLQ*ILIMASRLFGVG DAC*DLQVPS*KIETQSCSADSFGDTSIFLRDC FHLILAGPHQKIQPHTYIRQNIAVVPSVLKYCV LLWKHPLTLQ*PCKMNADYTDLMCYKIGESI**N LLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKAAGVNTTDKEIEVLYIRNV TFEDAGEYTCLAGNSIGISFHSAWLTVLPAPGR EKEITASPDYLEIAIYCIGVFLIACMVVTVILC RMKNTTKKPDFSSQPAVHKLTKRIPLRRQVSAES SSSMNSNTPLVRITTRLSSTADTPMLAGVSEYE LPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEA VGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSE MEMMKMIGKHKNIINLLGACTQDGPLYVIVEYA SKGNLREYLRARRPPGMEYSYDINRVPEEQMTF KDLVSCTYQLARGMEYLASQKCIHRDLAARNVLV TENNVMKIADFGLARDINNIDYYKKTTNGRLPV KWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLG GSPYPGIPVEELFKLLKEGHRMDKPANCTNELYM MMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNE EYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSP DPMPYEPCLPQYPHINGSVKT*MTVSACPQTGQH WEPSYTEQGDHASQSLLSPLVYMDQRSK*LEK* SAYV*RFIQLKTCNLPQEEKKVSGAVDCHKPPC NPSHLPCVLAVDQ*DSRWTCVLPSLLIL**LEKI YVSTHLQSTNAVYRCWMYVNIFKLCINIYYIFT RSYFLY*F*MDVPMHLENWSLFF**LFAKCCSY T*FLNFHRAEVEKYFCFQGKWYNVNLLINW*YTK QLIIYSFFCNLSGISMQAAQQTS*SIAWT*LVI RSFEKRIFTIYD*FGENEVLIYLCLNAAVRRLF LDLLNAPY*KNSFIGRCFILVCNPVITSTQRLTG LPKINGTSVLLKDALIHSLRTDLS*NDSRMCFS LAAGLLLLSCTLIRLACILFSEF**WLPDSLAL ETPVRIFKSHHRKLKHRVVLLIVLGIRPSF*GIA FI*FWQDLTKRSSLIPTSDKISPLFLLY*SIVF CFGNTHSLCNSRAR*MQITLILCVTKLEKVFNK TC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSRPPVLTPRTKRLRFSIFGM *LLRTLGNIRAWRVILLGYPFTLHG*QFCQRLEE KRRLQLPQTTWR*PFTA*GSS*SPVWW*QSSCA E*RTRPRSQTSAASRLCTS*PNVSPCGDRFRLS PAPP*TPTPRW*G*QHASLQRQTPPCWQGSPSMN FQRTQNGSFQEIS*HWASPWEKVALGKWSWRKQ WELTKTSPRRRSPWP*RC*KMMPQRKTFLIWCQ RWR**R*LGNTRIS*IFLEPAHRMGLSMS*LSMP LKATSENTSEPGGHPGWSTPMTLTVFLRSR*PS RTWCHAPTSWPEAWSTWLPKNVFIEI*QPEMFW *QKTM**K*QTLDSPEISTI*TITKRPPMGGFQS SGWLQKPCLIEYTLIRVMSGPSGC*CGRSSL*G ARPTQGFPWRNFLSC*RKDTEWISQPTAPTNCT ***GTVGMQCPPRDQRSSSW*KTWIEFSLSQPMR NTWTSANLSNSIHLVTLTQEVLVLQEMILFFLQ TPCLTNHAFLSIHT*TAVLKHE*LCLPVPKQDS TGNLATLSRETMPPRACCLHLYIWIRGVNNWKSN QHMCKDLYS*KLVIFPRRRRRFLEQWTATSHHV TPLTCRAYWLWTSRTQGGRAFCLPC*FCNNWRR FMSAHTYRAQMQYIGAGCM*IYSNYV*IYIIYLQ GVIFCIDFKWMSQCT*KIGLSFFNSYLLNAVLT HNFLIFTEQRWKNTFAFRENGITLIY**IGNIQ NN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN*LS DPLKREYLQYMTNLGKMKF*FICV*MLLSDDCS *TS*MPHIKRTHS*EGVSFWCATLSLRQRNV*L DFPR*MVPASS*KMP*SIP*GQTLVEMIAECASL WQLAFCF*VAH*SD*PVFSSVNFDNGFQTLWRW RRLLGSSSPIIEN*NTELFC**FWGYVHLFKGL LSSNSGRTSPKDPASYLHQTKYRRCSFCTKVLCF ALETPTHFAIAVQDECRLH*SYVLQNWRKYLIK PVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.kAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGA GGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGA AGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGT GCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGA GTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTAT GAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAG CAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTC AGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTAT GCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCT TCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTT GGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCA GTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAG GGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTA CATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAAT GAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTC CAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACA GCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAG TAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCAT GTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAA GATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTT ACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTT ACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATAC AAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTT ATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGT TCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAAC TGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTC TCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGT TGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGAT TGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTG TTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATA AAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQALGDK*FQCRSAGSVQCDR GGCWGIYM*GLQLYRAGQPVCLAHCPAKTASAW KRKGDYSFPRLPGDSHLLHRGLLNRLYGGNSHPV PNEEHDQEARLQQPAGCAQADQTYPPAETGFG* VQLLHELQHPAGEDNNTPLFNGRHPHAGRGLRV *TSRGPKMGVSKR*ADTGQAPGRRLLWASGHGGS SGN*QRQAQGGGHRGREDVER*CHRERPF*SGV RDGDDEDDWETQEYHKSSWSLHTGWASLCHS*V CL*RQPPRIPPSPEATRDGVLL*H*PCS*GADDL QGLGVMHLPAGQRHGVLGFPKMYSSRFSSQKCF GNRKQCDENSRLWTRQRYQQYRLLQKDHQWAAS SQVDGSRSPV**SIHSSE*CLVLRGVNVGDLHFR GLALPRDSRGGTF*AAEGRTQNG*ASQLHQRTV HDDEGLLACSALPETNVQAVGRRLGSNSHSHNQ *GILGPQPTSRTVFT*LP*HKKFLFFRR*FCFFS RPHALRTMPSSVSTHKRQC*NMNDCVCLSPNRT ALGT*LH*AGRPCLPELVVSTCIYGSEE*IIGK VISICVKIYTVENL*SSPGGEEGFWSSGLPQATM *PLSPAVRTGCGPVGLKVDVRSAFLVNFVIIGE DLCQHTLTEHKCSI*VLDVCKYIQIMYKYILYI YKELFFVLILNGCPNAPRKLVSLFLIAIC*MLFL HIIS*FSPSRGGKILLLSGKMV*R*FINKLVIY KTINHL*FFL*FKWHFYAGSTAD*LIYCLDLTS YQIL*KENIYNI*LIWGK*SFDLFVFKCCCQTIV LRPPKCPILKELIHRKVFHFGVQPCHYVNATSN WTSQDKWYQRPLKRCLNPFLEDRP*LK**QNVL LSGSWPSASELHINQISLYSLQ*ILIMASRLFGV GDAC*DLQVPS*KIETQSCSADSFGDTSIFLRD CFHLILAGPHQKIQPHTYIRQNIAVVPSVLKYC VLLWKHPLTLQ*PCKMNADYTDLMCYKIGESI** NLLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTE ADAGEYICKVSNYIGQANQSAWLTVLPKQQAPG REKEITASPDYLEIAIYCIGVFLIACMVVTVIL CRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVSAE SSSSMNSNTPLVRITTRLSSTADTPMLAGVSEY ELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAE AVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVS EMEMMKMIGKHKNIINLLGACTQDGPLYVIVEY ASKGNLREYLRARRPPGMEYSYDINRVPEEQMT FKDLVSCTYQLARGMEYLASQKCIHRDLAARNVL VTENNVMKIADFGLARDINNIDYYKKTTNGRLP VKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTL GGSPYPGIPVEELFKLLKEGHRMDKPANCTNELY MMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTN EEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFS PDPMPYEPCLPQYPHINGSVKT*MTVSACPQTGQ HWEPSYTEQGDHASQSLLSPLVYMDQRSK*LEK *SAYV*RFIQLKTCNLPQEEKKVSGAVDCHKPP CNPSHLPCVLAVDQ*DSRWTCVLPSLLIL**LEK IYVSTHLQSTNAVYRCWMYVNIFKLCINIYYIF TRSYFLY*F*MDVPMHLENWSLFF**LFAKCCS YT*FLNFHRAEVEKYFCFQGKWYNVNLLINW*YT KQLIIYSFFCNLSGISMQAAQQTS*SIAWT*LV IRSFEKRIFTIYD*FGENEVLIYLCLNAAVRRL FLDLLNAPY*KNSFIGRCFILVCNPVITSTQRLT GLPKINGTSVLLKDALIHSLRTDLS*NDSRMCF SLAAGLLLLSCTLIRLACILFSEF**WLPDSLA LETPVRIFKSHHRKLKHRVVLLIVLGIRPSF*GI AFI*FWQDLTKRSSLIPTSDKISPLFLLY*SIV FCFGNTHSLCNSRAR*MQITLILCVTKLEKVFN KTC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSSTRG*IVPMQKCWLCSM*P RRMLGNIYVRSPII*GRPTSLPGSLSCQNSKRLE EKRRLQLPQTTWR*PFTA*GSS*SPVWW*QSSC AE*RTRPRSQTSAASRLCTS*PNVSPCGDRFRL SPAPP*TPTPRW*G*QHASLQRQTPPCWQGSPSM NFQRTQNGSFQEIS*HWASPWEKVALGKWSWRK QWELTKTSPRRRSPWP*RC*KMMPQRKTFLIWC QRWR**R*LGNTRIS*IFLEPAHRMGLSMS*LSM PLKATSENTSEPGGHPGWSTPMTLTVFLRSR*P SRTWCHAPTSWPEAWSTWLPKNVFIEI*QPEMF W*QKTM**K*QTLDSPEISTI*TITKRPPMGGFQ SSGWLQKPCLIEYTLIRVMSGPSGC*CGRSSL* GARPTQGFPWRNFLSC*RKDTEWISQPTAPTNC T***GTVGMQCPPRDQRSSSW*KTWIEFSLSQPM RNTWTSANLSNSIHLVTLTQEVLVLQEMILFFL QTPCLTNHAFLSIHT*TAVLKHE*LCLPVPKQD STGNLATLSRETMPPRACCLHLYIWIRGVNNWKS NQHMCKDLYS*KLVIFPRRRRRFLEQWTATSHH VTPLTCRAYWLWTSRTQGGRAFCLPC*FCNNWR RFMSAHTYRAQMQYIGAGCM*IYSNYV*IYIIYL QGVIFCIDFKWMSQCT*KIGLSFFNSYLLNAVL THNFLIFTEQRWKNTFAFRENGITLIY**IGNI QNN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN*L SDPLKREYLQYMTNLGKMKF*FICV*MLLSDDC S*TS*MPHIKRTHS*EGVSFWCATLSLRQRNV* LDFPR*MVPASS*KMP*SIP*GQTLVEMIAECAS LWQLAFCF*VAH*SD*PVFSSVNFDNGFQTLWR WRRLLGSSSPIIEN*NTELFC**FWGYVHLFKG LLSSNSGRTSPKDPASYLHQTKYRRCSFCTKVLC FALETPTHFAIAVQDECRLH*SYVLQNWRKYLI KPVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.iAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGA GGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGA AGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGT GCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTC GGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCC GAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGG CGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCT GGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTT GAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGA TGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAA TGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGG CTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCA CTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGA ACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACA ACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTT TTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAAC AGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTG GAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGC CACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATT GGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTAT ATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCT GTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTA ATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAA CTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGAC GATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACG TCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATG TGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTT TGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTA AGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGT ATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTAT TTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQALGDK*FQCRSAGSVQCDR GGCWGIYM*GLQLYRAGQPVCLAHCPAKTASAW KRKGDYSFPRLPGDSHLLHRGLLNRLYGGNSHPV PNEEHDQEARLQQPAGCAQADQTYPPAETGNSF G*VQLLHELQHPAGEDNNTPLFNGRHPHAGRGL RV*TSRGPKMGVSKR*ADTGQAPGRRLLWASGHG GSSGN*QRQAQGGGHRGREDVER*CHRERPF*S GVRDGDDEDDWETQEYHKSSWSLHTGWASLCHS *VCL*RQPPRIPPSPEATRDGVLL*H*PCS*GAD DLQGLGVMHLPAGQRHGVLGFPKMYSSRFSSQK CFGNRKQCDENSRLWTRQRYQQYRLLQKDHQWA ASSQVDGSRSPV**SIHSSE*CLVLRGVNVGDLH FRGLALPRDSRGGTF*AAEGRTQNG*ASQLHQR TVHDDEGLLACSALPETNVQAVGRRLGSNSHSH NQ*GILGPQPTSRTVFT*LP*HKKFLFFRR*FCF FSRPHALRTMPSSVSTHKRQC*NMNDCVCLSPN RTALGT*LH*AGRPCLPELVVSTCIYGSEE*II GKVISICVKIYTVENL*SSPGGEEGFWSSGLPQA TM*PLSPAVRTGCGPVGLKVDVRSAFLVNFVII GEDLCQHTLTEHKCSI*VLDVCKYIQIMYKYIL YIYKELFFVLILNGCPNAPRKLVSLFLIAIC*ML FLHIIS*FSPSRGGKILLLSGKMV*R*FINKLV IYKTINHL*FFL*FKWHFYAGSTAD*LIYCLDL TSYQIL*KENIYNI*LIWGK*SFDLFVFKCCCQT IVLRPPKCPILKELIHRKVFHFGVQPCHYVNAT SNWTSQDKWYQRPLKRCLNPFLEDRP*LK**QN VLLSGSWPSASELHINQISLYSLQ*ILIMASRLF GVGDAC*DLQVPS*KIETQSCSADSFGDTSIFL RDCFHLILAGPHQKIQPHTYIRQNIAVVPSVLK YCVLLWKHPLTLQ*PCKMNADYTDLMCYKIGESI **NLLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTE ADAGEYICKVSNYIGQANQSAWLTVLPKQQAPG REKEITASPDYLEIAIYCIGVFLIACMVVTVIL CRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVTVS AESSSSMNSNTPLVRITTRLSSTADTPMLAGVS EYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVM AEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDL VSEMEMMKMIGKHKNIINLLGACTQDGPLYVIV EYASKGNLREYLRARRPPGMEYSYDINRVPEEQ MTFKDLVSCTYQLARGMEYLASQKCIHRDLAARN VLVTENNVMKIADFGLARDINNIDYYKKTTNGR LPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIF TLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNE LYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLT TNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSV FSPDPMPYEPCLPQYPHINGSVKT*MTVSACPQT GQHWEPSYTEQGDHASQSLLSPLVYMDQRSK*L EK*SAYV*RFIQLKTCNLPQEEKKVSGAVDCHK PPCNPSHLPCVLAVDQ*DSRWTCVLPSLLIL**L EKIYVSTHLQSTNAVYRCWMYVNIFKLCINIYY IFTRSYFLY*F*MDVPMHLENWSLFF**LFAKC CSYT*FLNFHRAEVEKYFCFQGKWYNVNLLINW* YTKQLIIYSFFCNLSGISMQAAQQTS*SIAWT* LVIRSFEKRIFTIYD*FGENEVLIYLCLNAAVR RLFLDLLNAPY*KNSFIGRCFILVCNPVITSTQR LTGLPKINGTSVLLKDALIHSLRTDLS*NDSRM CFSLAAGLLLLSCTLIRLACILFSEF**WLPDS LALETPVRIFKSHHRKLKHRVVLLIVLGIRPSF* GIAFI*FWQDLTKRSSLIPTSDKISPLFLLY*S IVFCFGNTHSLCNSRAR*MQITLILCVTKLEKV FNKTC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSSTRG*IVPMQKCWLCSM*P RRMLGNIYVRSPII*GRPTSLPGSLSCQNSKRLE EKRRLQLPQTTWR*PFTA*GSS*SPVWW*QSSC AE*RTRPRSQTSAASRLCTS*PNVSPCGDR*QF RLSPAPP*TPTPRW*G*QHASLQRQTPPCWQGSP SMNFQRTQNGSFQEIS*HWASPWEKVALGKWSW RKQWELTKTSPRRRSPWP*RC*KMMPQRKTFLI WCQRWR**R*LGNTRIS*IFLEPAHRMGLSMS*L SMPLKATSENTSEPGGHPGWSTPMTLTVFLRSR *PSRTWCHAPTSWPEAWSTWLPKNVFIEI*QPE MFW*QKTM**K*QTLDSPEISTI*TITKRPPMGG FQSSGWLQKPCLIEYTLIRVMSGPSGC*CGRSS L*GARPTQGFPWRNFLSC*RKDTEWISQPTAPT NCT***GTVGMQCPPRDQRSSSW*KTWIEFSLSQ PMRNTWTSANLSNSIHLVTLTQEVLVLQEMILF FLQTPCLTNHAFLSIHT*TAVLKHE*LCLPVPK QDSTGNLATLSRETMPPRACCLHLYIWIRGVNNW KSNQHMCKDLYS*KLVIFPRRRRRFLEQWTATS HHVTPLTCRAYWLWTSRTQGGRAFCLPC*FCNN WRRFMSAHTYRAQMQYIGAGCM*IYSNYV*IYII YLQGVIFCIDFKWMSQCT*KIGLSFFNSYLLNA VLTHNFLIFTEQRWKNTFAFRENGITLIY**IG NIQNN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN *LSDPLKREYLQYMTNLGKMKF*FICV*MLLSD DCS*TS*MPHIKRTHS*EGVSFWCATLSLRQRN V*LDFPR*MVPASS*KMP*SIP*GQTLVEMIAEC ASLWQLAFCF*VAH*SD*PVFSSVNFDNGFQTL WRWRRLLGSSSPIIEN*NTELFC**FWGYVHLF KGLLSSNSGRTSPKDPASYLHQTKYRRCSFCTKV LCFALETPTHFAIAVQDECRLH*SYVLQNWRKY LIKPVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.rAug05

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

Frame 1

Frame 2

Sequence FGFR2.qAug05

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

Frame 1

Frame 2

Sequence FGFR2.nAug05

AGGAAGTGTGCAGATGGGATTAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTG GTCGTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAA TCTCTCAACCAGAAGTGTACGTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGT GCACTTGGGGCCCAACAATAGGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGT AGGACTGTAGACAGTGAAACTTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTG TCAGTGAGAACAGTAACAACAAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTT TCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGA AACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGT ACCACCTGGATGTTGTGGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGC CTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATC CCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAG ACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGA AGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGAT GCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACAC AGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGA CATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGT ATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACT ATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTT CGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATG GATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAG ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTC TTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGA ATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTAT ATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGT TTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTG CCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATAT TCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTT TTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTAT AACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGA CTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTA TTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGC AACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAG ACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGA ATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATA GTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATAT CGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTT ATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATA AATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

RKCADGINVHMEIWKRTGDWYRNHGQLGSFHLP GRGHHGNLVPGPALLQFS*GYHIRARRATNQIPN LSTRSVRGCARGVARGALPVERCRRDQLD*GWG ALGAQQ*DSAYWGVLADKGRHA*RLRPLCLYCQ *DCRQ*NLVLHGECHRCHLIRR**G*HRWCGRFC Q*EQ*QQESTILDQHRKDGKAAPCCACGQHCQV SLPSRGEPNANHAVAEKREGV*AGASHWRLQGT KPALEPHYGKCGPI*QGKLYLCSGE*IRVHQSHV PPGCCGAWKRKGDYSFPRLPGDSHLLHRGLLNR LYGGNSHPVPNEEHDQEARLQQPAGCAQADQTY PPAETGNSFG*VQLLHELQHPAGEDNNTPLFNGR HPHAGRGLRV*TSRGPKMGVSKR*ADTGQAPGR RLLWASGHGGSSGN*QRQAQGGGHRGREDVER* CHRERPF*SGVRDGDDEDDWETQEYHKSSWSLHT GWASLCHS*VCL*RQPPRIPPSPEATRDGVLL* H*PCS*GADDLQGLGVMHLPAGQRHGVLGFPKM YSSRFSSQKCFGNRKQCDENSRLWTRQRYQQYRL LQKDHQWAASSQVDGSRSPV**SIHSSE*CLVL RGVNVGDLHFRGLALPRDSRGGTF*AAEGRTQN G*ASQLHQRTVHDDEGLLACSALPETNVQAVGRR LGSNSHSHNQ*GILGPQPTSRTVFT*LP*HKKF LFFRR*FCFFSRPHALRTMPSSVSTHKRQC*NM NDCVCLSPNRTALGT*LH*AGRPCLPELVVSTCI YGSEE*IIGKVISICVKIYTVENL*SSPGGEEG FWSSGLPQATM*PLSPAVRTGCGPVGLKVDVRS AFLVNFVIIGEDLCQHTLTEHKCSI*VLDVCKYI QIMYKYILYIYKELFFVLILNGCPNAPRKLVSL FLIAIC*MLFLHIIS*FSPSRGGKILLLSGKMV *R*FINKLVIYKTINHL*FFL*FKWHFYAGSTAD *LIYCLDLTSYQIL*KENIYNI*LIWGK*SFDL FVFKCCCQTIVLRPPKCPILKELIHRKVFHFGV QPCHYVNATSNWTSQDKWYQRPLKRCLNPFLEDR P*LK**QNVLLSGSWPSASELHINQISLYSLQ* ILIMASRLFGVGDAC*DLQVPS*KIETQSCSAD SFGDTSIFLRDCFHLILAGPHQKIQPHTYIRQNI AVVPSVLKYCVLLWKHPLTLQ*PCKMNADYTDL MCYKIGESI**NLLIFILTIKMFLQILMLTRQN KCHATYFF

Frame 1

GSVQMGLTSTWRYGRGPGIGTVTMVSWGRFICL VVVTMATLSLARPSFSLVEDTTLEPEEPPTKYQ ISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGV HLGPNNRTVLIGEYLQIKGATPRDSGLYACTAS RTVDSETWYFMVNVTDAISSGDDEDDTDGAEDF VSENSNNKRAPYWTNTEKMEKRLHAVPAANTVKF RCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVR NQHWSLIMESVVPSDKGNYTCVVENEYGSINHT YHLDVVAPGREKEITASPDYLEIAIYCIGVFLIA CMVVTVILCRMKNTTKKPDFSSQPAVHKLTKRI PLRRQVTVSAESSSSMNSNTPLVRITTRLSSTA DTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGE GCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDD ATEKDLSDLVSEMEMMKMIGKHKNIINLLGACT QDGPLYVIVEYASKGNLREYLRARRPPGMEYSYD INRVPEEQMTFKDLVSCTYQLARGMEYLASQKC IHRDLAARNVLVTENNVMKIADFGLARDINNID YYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSF GVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRM DKPANCTNELYMMMRDCWHAVPSQRPTFKQLVE DLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSS CSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT* MTVSACPQTGQHWEPSYTEQGDHASQSLLSPLV YMDQRSK*LEK*SAYV*RFIQLKTCNLPQEEKKV SGAVDCHKPPCNPSHLPCVLAVDQ*DSRWTCVL PSLLIL**LEKIYVSTHLQSTNAVYRCWMYVNI FKLCINIYYIFTRSYFLY*F*MDVPMHLENWSLF F**LFAKCCSYT*FLNFHRAEVEKYFCFQGKWY NVNLLINW*YTKQLIIYSFFCNLSGISMQAAQQ TS*SIAWT*LVIRSFEKRIFTIYD*FGENEVLIY LCLNAAVRRLFLDLLNAPY*KNSFIGRCFILVC NPVITSTQRLTGLPKINGTSVLLKDALIHSLRT DLS*NDSRMCFSLAAGLLLLSCTLIRLACILFSE F**WLPDSLALETPVRIFKSHHRKLKHRVVLLI VLGIRPSF*GIAFI*FWQDLTKRSSLIPTSDKI SPLFLLY*SIVFCFGNTHSLCNSRAR*MQITLIL CVTKLEKVFNKTC*FLY*Q*KCFYRY*C*QDKI NVTQLIFL

Frame 2

EVCRWD*RPHGDMEEDRGLVP*PWSAGVVSSAW SWSPWQPCPWPGPPSV*LRIPH*SQKSHQPNTK SLNQKCTWLRQGSR*RCAAC*KMPP*SVGLRMG CTWGPTIGQCLLGSTCR*RAPRLETPASMLVLPV GL*TVKLGTSW*MSQMPSHPEMMRMTPMVRKIL SVRTVTTREHHTGPTQKRWKSGSMLCLRPTLSS FAAQPGGTQCQPCGG*KTGRSLSRSIALEATRYE TSTGASLWKVWSHLTREIIPV*WRMNTGPSITR TTWMLWRLEEKRRLQLPQTTWR*PFTA*GSS*S PVWW*QSSCAE*RTRPRSQTSAASRLCTS*PNVS PCGDR*QFRLSPAPP*TPTPRW*G*QHASLQRQ TPPCWQGSPSMNFQRTQNGSFQEIS*HWASPWE KVALGKWSWRKQWELTKTSPRRRSPWP*RC*KMM PQRKTFLIWCQRWR**R*LGNTRIS*IFLEPAH RMGLSMS*LSMPLKATSENTSEPGGHPGWSTPM TLTVFLRSR*PSRTWCHAPTSWPEAWSTWLPKNV FIEI*QPEMFW*QKTM**K*QTLDSPEISTI*T ITKRPPMGGFQSSGWLQKPCLIEYTLIRVMSGP SGC*CGRSSL*GARPTQGFPWRNFLSC*RKDTEW ISQPTAPTNCT***GTVGMQCPPRDQRSSSW*K TWIEFSLSQPMRNTWTSANLSNSIHLVTLTQEV LVLQEMILFFLQTPCLTNHAFLSIHT*TAVLKHE *LCLPVPKQDSTGNLATLSRETMPPRACCLHLY IWIRGVNNWKSNQHMCKDLYS*KLVIFPRRRRR FLEQWTATSHHVTPLTCRAYWLWTSRTQGGRAFC LPC*FCNNWRRFMSAHTYRAQMQYIGAGCM*IY SNYV*IYIIYLQGVIFCIDFKWMSQCT*KIGLS FFNSYLLNAVLTHNFLIFTEQRWKNTFAFRENGI TLIY**IGNIQNN*SFIVFFVI*VAFLCRQHSR LVNLLLGLN*LSDPLKREYLQYMTNLGKMKF*F ICV*MLLSDDCS*TS*MPHIKRTHS*EGVSFWCA TLSLRQRNV*LDFPR*MVPASS*KMP*SIP*GQ TLVEMIAECASLWQLAFCF*VAH*SD*PVFSSV NFDNGFQTLWRWRRLLGSSSPIIEN*NTELFC** FWGYVHLFKGLLSSNSGRTSPKDPASYLHQTKY RRCSFCTKVLCFALETPTHFAIAVQDECRLH*S YVLQNWRKYLIKPVNFYTDNKNVSTDINVNKTK* MSRNLFF*

Sequence FGFR2.jAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGTTTCGGCTGA GTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTAT GAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAG CAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTC AGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTAT GCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCT TCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTT GGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCA GTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAG GGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTA CATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAAT GAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTC CAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACA GCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAG TAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCAT GTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAA GATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTT ACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTT ACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATAC AAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTT ATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGT TCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAAC TGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTC TCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGT TGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGAT TGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTG TTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATA AAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGFG* VQLLHELQHPAGEDNNTPLFNGRHPHAGRGLRV *TSRGPKMGVSKR*ADTGQAPGRRLLWASGHGGS SGN*QRQAQGGGHRGREDVER*CHRERPF*SGV RDGDDEDDWETQEYHKSSWSLHTGWASLCHS*V CL*RQPPRIPPSPEATRDGVLL*H*PCS*GADDL QGLGVMHLPAGQRHGVLGFPKMYSSRFSSQKCF GNRKQCDENSRLWTRQRYQQYRLLQKDHQWAAS SQVDGSRSPV**SIHSSE*CLVLRGVNVGDLHFR GLALPRDSRGGTF*AAEGRTQNG*ASQLHQRTV HDDEGLLACSALPETNVQAVGRRLGSNSHSHNQ *GILGPQPTSRTVFT*LP*HKKFLFFRR*FCFFS RPHALRTMPSSVSTHKRQC*NMNDCVCLSPNRT ALGT*LH*AGRPCLPELVVSTCIYGSEE*IIGK VISICVKIYTVENL*SSPGGEEGFWSSGLPQATM *PLSPAVRTGCGPVGLKVDVRSAFLVNFVIIGE DLCQHTLTEHKCSI*VLDVCKYIQIMYKYILYI YKELFFVLILNGCPNAPRKLVSLFLIAIC*MLFL HIIS*FSPSRGGKILLLSGKMV*R*FINKLVIY KTINHL*FFL*FKWHFYAGSTAD*LIYCLDLTS YQIL*KENIYNI*LIWGK*SFDLFVFKCCCQTIV LRPPKCPILKELIHRKVFHFGVQPCHYVNATSN WTSQDKWYQRPLKRCLNPFLEDRP*LK**QNVL LSGSWPSASELHINQISLYSLQ*ILIMASRLFGV GDAC*DLQVPS*KIETQSCSADSFGDTSIFLRD CFHLILAGPHQKIQPHTYIRQNIAVVPSVLKYC VLLWKHPLTLQ*PCKMNADYTDLMCYKIGESI** NLLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKVSAE SSSSMNSNTPLVRITTRLSSTADTPMLAGVSEY ELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAE AVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVS EMEMMKMIGKHKNIINLLGACTQDGPLYVIVEY ASKGNLREYLRARRPPGMEYSYDINRVPEEQMT FKDLVSCTYQLARGMEYLASQKCIHRDLAARNVL VTENNVMKIADFGLARDINNIDYYKKTTNGRLP VKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTL GGSPYPGIPVEELFKLLKEGHRMDKPANCTNELY MMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTN EEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFS PDPMPYEPCLPQYPHINGSVKT*MTVSACPQTGQ HWEPSYTEQGDHASQSLLSPLVYMDQRSK*LEK *SAYV*RFIQLKTCNLPQEEKKVSGAVDCHKPP CNPSHLPCVLAVDQ*DSRWTCVLPSLLIL**LEK IYVSTHLQSTNAVYRCWMYVNIFKLCINIYYIF TRSYFLY*F*MDVPMHLENWSLFF**LFAKCCS YT*FLNFHRAEVEKYFCFQGKWYNVNLLINW*YT KQLIIYSFFCNLSGISMQAAQQTS*SIAWT*LV IRSFEKRIFTIYD*FGENEVLIYLCLNAAVRRL FLDLLNAPY*KNSFIGRCFILVCNPVITSTQRLT GLPKINGTSVLLKDALIHSLRTDLS*NDSRMCF SLAAGLLLLSCTLIRLACILFSEF**WLPDSLA LETPVRIFKSHHRKLKHRVVLLIVLGIRPSF*GI AFI*FWQDLTKRSSLIPTSDKISPLFLLY*SIV FCFGNTHSLCNSRAR*MQITLILCVTKLEKVFN KTC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRFRL SPAPP*TPTPRW*G*QHASLQRQTPPCWQGSPSM NFQRTQNGSFQEIS*HWASPWEKVALGKWSWRK QWELTKTSPRRRSPWP*RC*KMMPQRKTFLIWC QRWR**R*LGNTRIS*IFLEPAHRMGLSMS*LSM PLKATSENTSEPGGHPGWSTPMTLTVFLRSR*P SRTWCHAPTSWPEAWSTWLPKNVFIEI*QPEMF W*QKTM**K*QTLDSPEISTI*TITKRPPMGGFQ SSGWLQKPCLIEYTLIRVMSGPSGC*CGRSSL* GARPTQGFPWRNFLSC*RKDTEWISQPTAPTNC T***GTVGMQCPPRDQRSSSW*KTWIEFSLSQPM RNTWTSANLSNSIHLVTLTQEVLVLQEMILFFL QTPCLTNHAFLSIHT*TAVLKHE*LCLPVPKQD STGNLATLSRETMPPRACCLHLYIWIRGVNNWKS NQHMCKDLYS*KLVIFPRRRRRFLEQWTATSHH VTPLTCRAYWLWTSRTQGGRAFCLPC*FCNNWR RFMSAHTYRAQMQYIGAGCM*IYSNYV*IYIIYL QGVIFCIDFKWMSQCT*KIGLSFFNSYLLNAVL THNFLIFTEQRWKNTFAFRENGITLIY**IGNI QNN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN*L SDPLKREYLQYMTNLGKMKF*FICV*MLLSDDC S*TS*MPHIKRTHS*EGVSFWCATLSLRQRNV* LDFPR*MVPASS*KMP*SIP*GQTLVEMIAECAS LWQLAFCF*VAH*SD*PVFSSVNFDNGFQTLWR WRRLLGSSSPIIEN*NTELFC**FWGYVHLFKG LLSSNSGRTSPKDPASYLHQTKYRRCSFCTKVLC FALETPTHFAIAVQDECRLH*SYVLQNWRKYLI KPVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.fAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGC CTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGG GCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAA GATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTT CTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGA TGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTT GGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGAT ATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGA GTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAA GGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTC AAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGATCTGAAAGTTTATGGCTTCATTGAGAAACTGGGAAAAGTTGGTCAGGC GCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGATCATGAGGTCAGGAGTTCCAGACCAGCCTGGCCAACATGGTGAAACCC TGTCTCTACTAAAGATACAAAAAATTAGCCGGGCGTGTTGGTGTGCACCTGTAATCCCAGCTACTCCGGGAGGCTGAGGCAGGAGAGTCACTTGAACCGG GGAGGCGGAGGTTGCAGTGAGCCGAGATCATGCCATTGCATTCCAGCCTTGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAT

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGNSFG*VQLLHELQHPAGEDNNT PLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTG QAPGRRLLWASGHGGSSGN*QRQAQGGGHRGRE DVER*CHRERPF*SGVRDGDDEDDWETQEYHKS SWSLHTGWASLCHS*VCL*RQPPRIPPSPEATRD GVLL*H*PCS*GADDLQGLGVMHLPAGQRHGVL GFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQR YQQYRLLQKDHQWAASSQVDGSRSPV**SIHSSE *CLVLRGVNVGDLHFRGLALPRDSRGGTF*AAE GRTQNG*ASQLHQRTVHDDEGLLACSALPETNV QAVGRRLGSNSHSHNQ*DLKVYGFIEKLGKVGQA QWLMPVIPALWEAEAGGS*GQEFQTSLANMVKP CLY*RYKKLAGRVGVHL*SQLLREAEAGESLEP GRRRLQ*AEIMPLHSSLGDRARLRLKKKKN

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVTVSAESSSSMNSNTPLVRITTR LSSTADTPMLAGVSEYELPEDPKWEFPRDKLTL GKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVK MLKDDATEKDLSDLVSEMEMMKMIGKHKNIINL LGACTQDGPLYVIVEYASKGNLREYLRARRPPG MEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYL ASQKCIHRDLAARNVLVTENNVMKIADFGLARD INNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQ SDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLK EGHRMDKPANCTNELYMMMRDCWHAVPSQRPTF KQLVEDLDRILTLTTNEI*KFMASLRNWEKLVR RSGSCL*SQHFGRPRQADHEVRSSRPAWPTW*NP VSTKDTKN*PGVLVCTCNPSYSGRLRQESHLNR GGGGCSEPRSCHCIPALATERDSVSKKKK

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QHA SLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*HW ASPWEKVALGKWSWRKQWELTKTSPRRRSPWP* RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*IF LEPAHRMGLSMS*LSMPLKATSENTSEPGGHPG WSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWST WLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPEI STI*TITKRPPMGGFQSSGWLQKPCLIEYTLIR VMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC* RKDTEWISQPTAPTNCT***GTVGMQCPPRDQRS SSW*KTWIEFSLSQPMRSESLWLH*ETGKSWSG AVAHACNPSTLGGRGRRIMRSGVPDQPGQHGET LSLLKIQKISRACWCAPVIPATPGG*GRRVT*TG EAEVAVSRDHAIAFQPWRQSETPSQKKKK

Sequence FGFR2.eAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGAT AAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGAGGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAAC CAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCA TAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCA CAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACA CGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACAC TGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGT GAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAAT CTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCG GGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTA CTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGA GATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATC AGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCT GAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACG TTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGATCTGAAAGTTTATGGCTTCATTGAGAAACTGGGAAAAGTTGGTCA GGCGCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGATCATGAGGTCAGGAGTTCCAGACCAGCCTGGCCAACATGGTGAAA CCCTGTCTCTACTAAAGATACAAAAAATTAGCCGGGCGTGTTGGTGTGCACCTGTAATCCCAGCTACTCCGGGAGGCTGAGGCAGGAGAGTCACTTGAAC CGGGGAGGCGGAGGTTGCAGTGAGCCGAGATCATGCCATTGCATTCCAGCCTTGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAT

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQALGD K*FQCRSAGSVQCDRGGCWGIYM*GLQLYRAGQ PVCLAHCPAKTASAWKRKGDYSFPRLPGDSHLLH RGLLNRLYGGNSHPVPNEEHDQEARLQQPAGCA QADQTYPPAETGNSFG*VQLLHELQHPAGEDNN TPLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADT GQAPGRRLLWASGHGGSSGN*QRQAQGGGHRGR EDVER*CHRERPF*SGVRDGDDEDDWETQEYHK SSWSLHTGWASLCHS*VCL*RQPPRIPPSPEATR DGVLL*H*PCS*GADDLQGLGVMHLPAGQRHGV LGFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQ RYQQYRLLQKDHQWAASSQVDGSRSPV**SIHSS E*CLVLRGVNVGDLHFRGLALPRDSRGGTF*AA EGRTQNG*ASQLHQRTVHDDEGLLACSALPETN VQAVGRRLGSNSHSHNQ*DLKVYGFIEKLGKVGQ AQWLMPVIPALWEAEAGGS*GQEFQTSLANMVK PCLY*RYKKLAGRVGVHL*SQLLREAEAGESLE PGRRRLQ*AEIMPLHSSLGDRARLRLKKKKN

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGI NSSNAEVLALFNVTEADAGEYICKVSNYIGQAN QSAWLTVLPKQQAPGREKEITASPDYLEIAIYC IGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVH KLTKRIPLRRQVTVSAESSSSMNSNTPLVRITT RLSSTADTPMLAGVSEYELPEDPKWEFPRDKLT LGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAV KMLKDDATEKDLSDLVSEMEMMKMIGKHKNIIN LLGACTQDGPLYVIVEYASKGNLREYLRARRPP GMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEY LASQKCIHRDLAARNVLVTENNVMKIADFGLAR DINNIDYYKKTTNGRLPVKWMAPEALFDRVYTH QSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL KEGHRMDKPANCTNELYMMMRDCWHAVPSQRPT FKQLVEDLDRILTLTTNEI*KFMASLRNWEKLV RRSGSCL*SQHFGRPRQADHEVRSSRPAWPTW*N PVSTKDTKN*PGVLVCTCNPSYSGRLRQESHLN RGGGGCSEPRSCHCIPALATERDSVSKKKK

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSSTRG *IVPMQKCWLCSM*PRRMLGNIYVRSPII*GRPT SLPGSLSCQNSKRLEEKRRLQLPQTTWR*PFTA *GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLC TS*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QH ASLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*H WASPWEKVALGKWSWRKQWELTKTSPRRRSPWP *RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*I FLEPAHRMGLSMS*LSMPLKATSENTSEPGGHP GWSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWS TWLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPE ISTI*TITKRPPMGGFQSSGWLQKPCLIEYTLI RVMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC *RKDTEWISQPTAPTNCT***GTVGMQCPPRDQR SSSW*KTWIEFSLSQPMRSESLWLH*ETGKSWS GAVAHACNPSTLGGRGRRIMRSGVPDQPGQHGE TLSLLKIQKISRACWCAPVIPATPGG*GRRVT*T GEAEVAVSRDHAIAFQPWRQSETPSQKKKK

Sequence FGFR2.dAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCT TCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGC CCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTT GAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGA GCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGT ACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTC CCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAAC AATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATG TCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGG ACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAG TTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGTCAGCCTCTCGAACCGTATTCACCTTGTTATCCTGACC CAAGATGAAATAAAACGTCTCTCTTCCCTTCTTTCAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGT TCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACAT GAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGT ATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAG GTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTC TGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATAT ATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTC TTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGT ATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCA GACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATT TATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGT GCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGAC AGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGT GAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGA TAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAAT ATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATC TTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAA TAAATGTCACGCAACTT

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGFG*VQLLHELQHPAGEDNNTPL FNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTGQA PGRRLLWASGHGGSSGN*QRQAQGGGHRGREDV ER*CHRERPF*SGVRDGDDEDDWETQEYHKSSW SLHTGWASLCHS*VCL*RQPPRIPPSPEATRDGV LL*H*PCS*GADDLQGLGVMHLPAGQRHGVLGF PKMYSSRFSSQKCFGNRKQCDENSRLWTRQRYQ QYRLLQKDHQWAASSQVDGSRSPV**SIHSSE*C LVLRGVNVGDLHFRGLALPRDSRGGTF*AAEGR TQNG*ASQLHQRTVHDDEGLLACSALPETNVQA VGRRLGSNSHSHNQ*GILGPQSASRTVFTLLS*P KMK*NVSLPFFQEYLDLSQPLEQYSPSYPDTRS SCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT *MTVSACPQTGQHWEPSYTEQGDHASQSLLSPLV YMDQRSK*LEK*SAYV*RFIQLKTCNLPQEEKK VSGAVDCHKPPCNPSHLPCVLAVDQ*DSRWTCV LPSLLIL**LEKIYVSTHLQSTNAVYRCWMYVNI FKLCINIYYIFTRSYFLY*F*MDVPMHLENWSL FF**LFAKCCSYT*FLNFHRAEVEKYFCFQGKW YNVNLLINW*YTKQLIIYSFFCNLSGISMQAAQQ TS*SIAWT*LVIRSFEKRIFTIYD*FGENEVLI YLCLNAAVRRLFLDLLNAPY*KNSFIGRCFILV CNPVITSTQRLTGLPKINGTSVLLKDALIHSLRT DLS*NDSRMCFSLAAGLLLLSCTLIRLACILFS EF**WLPDSLALETPVRIFKSHHRKLKHRVVLL IVLGIRPSF*GIAFI*FWQDLTKRSSLIPTSDKI SPLFLLY*SIVFCFGNTHSLCNSRAR*MQITLI LCVTKLEKVFNKTC*FLY*Q*KCFYRY*C*QDK INVTQL

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVSAESSSSMNSNTPLVRITTRLS STADTPMLAGVSEYELPEDPKWEFPRDKLTLGK PLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKML KDDATEKDLSDLVSEMEMMKMIGKHKNIINLLG ACTQDGPLYVIVEYASKGNLREYLRARRPPGME YSYDINRVPEEQMTFKDLVSCTYQLARGMEYLAS QKCIHRDLAARNVLVTENNVMKIADFGLARDIN NIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSD VWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEG HRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQ LVEDLDRILTLTTNEEYLDLSQPLEPYSPCYPD PR*NKTSLFPSFRNTWTSANLSNSIHLVTLTQEV LVLQEMILFFLQTPCLTNHAFLSIHT*TAVLKH E*LCLPVPKQDSTGNLATLSRETMPPRACCLHL YIWIRGVNNWKSNQHMCKDLYS*KLVIFPRRRRR FLEQWTATSHHVTPLTCRAYWLWTSRTQGGRAF CLPC*FCNNWRRFMSAHTYRAQMQYIGAGCM*I YSNYV*IYIIYLQGVIFCIDFKWMSQCT*KIGLS FFNSYLLNAVLTHNFLIFTEQRWKNTFAFRENG ITLIY**IGNIQNN*SFIVFFVI*VAFLCRQHS RLVNLLLGLN*LSDPLKREYLQYMTNLGKMKF*F ICV*MLLSDDCS*TS*MPHIKRTHS*EGVSFWC ATLSLRQRNV*LDFPR*MVPASS*KMP*SIP*G QTLVEMIAECASLWQLAFCF*VAH*SD*PVFSSV NFDNGFQTLWRWRRLLGSSSPIIEN*NTELFC* *FWGYVHLFKGLLSSNSGRTSPKDPASYLHQTK YRRCSFCTKVLCFALETPTHFAIAVQDECRLH*S YVLQNWRKYLIKPVNFYTDNKNVSTDINVNKTK *MSRN

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDRFRLSPAPP*TPTPRW*G*QHASL QRQTPPCWQGSPSMNFQRTQNGSFQEIS*HWAS PWEKVALGKWSWRKQWELTKTSPRRRSPWP*RC *KMMPQRKTFLIWCQRWR**R*LGNTRIS*IFLE PAHRMGLSMS*LSMPLKATSENTSEPGGHPGWS TPMTLTVFLRSR*PSRTWCHAPTSWPEAWSTWL PKNVFIEI*QPEMFW*QKTM**K*QTLDSPEIST I*TITKRPPMGGFQSSGWLQKPCLIEYTLIRVM SGPSGC*CGRSSL*GARPTQGFPWRNFLSC*RK DTEWISQPTAPTNCT***GTVGMQCPPRDQRSSS W*KTWIEFSLSQPMRNTWTSVSLSNRIHLVILT QDEIKRLSSLLSGILGPQPTSRTVFT*LP*HKK FLFFRR*FCFFSRPHALRTMPSSVSTHKRQC*NM NDCVCLSPNRTALGT*LH*AGRPCLPELVVSTC IYGSEE*IIGKVISICVKIYTVENL*SSPGGEE GFWSSGLPQATM*PLSPAVRTGCGPVGLKVDVRS AFLVNFVIIGEDLCQHTLTEHKCSI*VLDVCKY IQIMYKYILYIYKELFFVLILNGCPNAPRKLVS LFLIAIC*MLFLHIIS*FSPSRGGKILLLSGKMV *R*FINKLVIYKTINHL*FFL*FKWHFYAGSTA D*LIYCLDLTSYQIL*KENIYNI*LIWGK*SFD LFVFKCCCQTIVLRPPKCPILKELIHRKVFHFGV QPCHYVNATSNWTSQDKWYQRPLKRCLNPFLED RP*LK**QNVLLSGSWPSASELHINQISLYSLQ *ILIMASRLFGVGDAC*DLQVPS*KIETQSCSAD SFGDTSIFLRDCFHLILAGPHQKIQPHTYIRQN IAVVPSVLKYCVLLWKHPLTLQ*PCKMNADYTD LMCYKIGESI**NLLIFILTIKMFLQILMLTRQN KCHAT

Sequence FGFR2.cAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCT TCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGC CCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTT GAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGA GCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGT ACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTC CCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAAC AATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATG TCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGG ACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAG TTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACA CAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGT TAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCT CCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGG AGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGAC GTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTAT GTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATT GGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGG AAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCA GCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAG TTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCAT TTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCC TTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTC TCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGT TCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCA GACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTA CACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACA AGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGFG*VQLLHELQHPAGEDNNTPL FNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTGQA PGRRLLWASGHGGSSGN*QRQAQGGGHRGREDV ER*CHRERPF*SGVRDGDDEDDWETQEYHKSSW SLHTGWASLCHS*VCL*RQPPRIPPSPEATRDGV LL*H*PCS*GADDLQGLGVMHLPAGQRHGVLGF PKMYSSRFSSQKCFGNRKQCDENSRLWTRQRYQ QYRLLQKDHQWAASSQVDGSRSPV**SIHSSE*C LVLRGVNVGDLHFRGLALPRDSRGGTF*AAEGR TQNG*ASQLHQRTVHDDEGLLACSALPETNVQA VGRRLGSNSHSHNQ*GILGPQPTSRTVFT*LP*H KKFLFFRR*FCFFSRPHALRTMPSSVSTHKRQC *NMNDCVCLSPNRTALGT*LH*AGRPCLPELVV STCIYGSEE*IIGKVISICVKIYTVENL*SSPGG EEGFWSSGLPQATM*PLSPAVRTGCGPVGLKVD VRSAFLVNFVIIGEDLCQHTLTEHKCSI*VLDV CKYIQIMYKYILYIYKELFFVLILNGCPNAPRKL VSLFLIAIC*MLFLHIIS*FSPSRGGKILLLSG KMV*R*FINKLVIYKTINHL*FFL*FKWHFYAG STAD*LIYCLDLTSYQIL*KENIYNI*LIWGK*S FDLFVFKCCCQTIVLRPPKCPILKELIHRKVFH FGVQPCHYVNATSNWTSQDKWYQRPLKRCLNPF LEDRP*LK**QNVLLSGSWPSASELHINQISLYS LQ*ILIMASRLFGVGDAC*DLQVPS*KIETQSC SADSFGDTSIFLRDCFHLILAGPHQKIQPHTYI RQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMNADY TDLMCYKIGESI**NLLIFILTIKMFLQILMLT RQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVSAESSSSMNSNTPLVRITTRLS STADTPMLAGVSEYELPEDPKWEFPRDKLTLGK PLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKML KDDATEKDLSDLVSEMEMMKMIGKHKNIINLLG ACTQDGPLYVIVEYASKGNLREYLRARRPPGME YSYDINRVPEEQMTFKDLVSCTYQLARGMEYLAS QKCIHRDLAARNVLVTENNVMKIADFGLARDIN NIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSD VWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEG HRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQ LVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPD TRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSV KT*MTVSACPQTGQHWEPSYTEQGDHASQSLLS PLVYMDQRSK*LEK*SAYV*RFIQLKTCNLPQE EKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DSRWT CVLPSLLIL**LEKIYVSTHLQSTNAVYRCWMY VNIFKLCINIYYIFTRSYFLY*F*MDVPMHLEN WSLFF**LFAKCCSYT*FLNFHRAEVEKYFCFQG KWYNVNLLINW*YTKQLIIYSFFCNLSGISMQA AQQTS*SIAWT*LVIRSFEKRIFTIYD*FGENE VLIYLCLNAAVRRLFLDLLNAPY*KNSFIGRCFI LVCNPVITSTQRLTGLPKINGTSVLLKDALIHS LRTDLS*NDSRMCFSLAAGLLLLSCTLIRLACI LFSEF**WLPDSLALETPVRIFKSHHRKLKHRVV LLIVLGIRPSF*GIAFI*FWQDLTKRSSLIPTS DKISPLFLLY*SIVFCFGNTHSLCNSRAR*MQI TLILCVTKLEKVFNKTC*FLY*Q*KCFYRY*C*Q DKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDRFRLSPAPP*TPTPRW*G*QHASL QRQTPPCWQGSPSMNFQRTQNGSFQEIS*HWAS PWEKVALGKWSWRKQWELTKTSPRRRSPWP*RC *KMMPQRKTFLIWCQRWR**R*LGNTRIS*IFLE PAHRMGLSMS*LSMPLKATSENTSEPGGHPGWS TPMTLTVFLRSR*PSRTWCHAPTSWPEAWSTWL PKNVFIEI*QPEMFW*QKTM**K*QTLDSPEIST I*TITKRPPMGGFQSSGWLQKPCLIEYTLIRVM SGPSGC*CGRSSL*GARPTQGFPWRNFLSC*RK DTEWISQPTAPTNCT***GTVGMQCPPRDQRSSS W*KTWIEFSLSQPMRNTWTSANLSNSIHLVTLT QEVLVLQEMILFFLQTPCLTNHAFLSIHT*TAV LKHE*LCLPVPKQDSTGNLATLSRETMPPRACCL HLYIWIRGVNNWKSNQHMCKDLYS*KLVIFPRR RRRFLEQWTATSHHVTPLTCRAYWLWTSRTQGG RAFCLPC*FCNNWRRFMSAHTYRAQMQYIGAGCM *IYSNYV*IYIIYLQGVIFCIDFKWMSQCT*KI GLSFFNSYLLNAVLTHNFLIFTEQRWKNTFAFR ENGITLIY**IGNIQNN*SFIVFFVI*VAFLCRQ HSRLVNLLLGLN*LSDPLKREYLQYMTNLGKMK F*FICV*MLLSDDCS*TS*MPHIKRTHS*EGVS FWCATLSLRQRNV*LDFPR*MVPASS*KMP*SIP *GQTLVEMIAECASLWQLAFCF*VAH*SD*PVF SSVNFDNGFQTLWRWRRLLGSSSPIIEN*NTEL FC**FWGYVHLFKGLLSSNSGRTSPKDPASYLHQ TKYRRCSFCTKVLCFALETPTHFAIAVQDECRL H*SYVLQNWRKYLIKPVNFYTDNKNVSTDINVN KTK*MSRNLFF*

Sequence FGFR2.bAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGC CTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGG GCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAA GATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTT CTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGA TGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTT GGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGAT ATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGA GTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAA GGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTC AAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACC CTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGG CAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTG TTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCC AGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAG GTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGG ATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAG AAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTT TCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATG CAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAA ATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTG TTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATC CATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCT GTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAG AGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCT ACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGC AGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATG TTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGNSFG*VQLLHELQHPAGEDNNT PLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTG QAPGRRLLWASGHGGSSGN*QRQAQGGGHRGRE DVER*CHRERPF*SGVRDGDDEDDWETQEYHKS SWSLHTGWASLCHS*VCL*RQPPRIPPSPEATRD GVLL*H*PCS*GADDLQGLGVMHLPAGQRHGVL GFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQR YQQYRLLQKDHQWAASSQVDGSRSPV**SIHSSE *CLVLRGVNVGDLHFRGLALPRDSRGGTF*AAE GRTQNG*ASQLHQRTVHDDEGLLACSALPETNV QAVGRRLGSNSHSHNQ*GILGPQPTSRTVFT*LP *HKKFLFFRR*FCFFSRPHALRTMPSSVSTHKR QC*NMNDCVCLSPNRTALGT*LH*AGRPCLPEL VVSTCIYGSEE*IIGKVISICVKIYTVENL*SSP GGEEGFWSSGLPQATM*PLSPAVRTGCGPVGLK VDVRSAFLVNFVIIGEDLCQHTLTEHKCSI*VL DVCKYIQIMYKYILYIYKELFFVLILNGCPNAPR KLVSLFLIAIC*MLFLHIIS*FSPSRGGKILLL SGKMV*R*FINKLVIYKTINHL*FFL*FKWHFY AGSTAD*LIYCLDLTSYQIL*KENIYNI*LIWGK *SFDLFVFKCCCQTIVLRPPKCPILKELIHRKV FHFGVQPCHYVNATSNWTSQDKWYQRPLKRCLN PFLEDRP*LK**QNVLLSGSWPSASELHINQISL YSLQ*ILIMASRLFGVGDAC*DLQVPS*KIETQ SCSADSFGDTSIFLRDCFHLILAGPHQKIQPHT YIRQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMNA DYTDLMCYKIGESI**NLLIFILTIKMFLQILM LTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVTVSAESSSSMNSNTPLVRITTR LSSTADTPMLAGVSEYELPEDPKWEFPRDKLTL GKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVK MLKDDATEKDLSDLVSEMEMMKMIGKHKNIINL LGACTQDGPLYVIVEYASKGNLREYLRARRPPG MEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYL ASQKCIHRDLAARNVLVTENNVMKIADFGLARD INNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQ SDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLK EGHRMDKPANCTNELYMMMRDCWHAVPSQRPTF KQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSY PDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHING SVKT*MTVSACPQTGQHWEPSYTEQGDHASQSL LSPLVYMDQRSK*LEK*SAYV*RFIQLKTCNLP QEEKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DSR WTCVLPSLLIL**LEKIYVSTHLQSTNAVYRCW MYVNIFKLCINIYYIFTRSYFLY*F*MDVPMHL ENWSLFF**LFAKCCSYT*FLNFHRAEVEKYFCF QGKWYNVNLLINW*YTKQLIIYSFFCNLSGISM QAAQQTS*SIAWT*LVIRSFEKRIFTIYD*FGE NEVLIYLCLNAAVRRLFLDLLNAPY*KNSFIGRC FILVCNPVITSTQRLTGLPKINGTSVLLKDALI HSLRTDLS*NDSRMCFSLAAGLLLLSCTLIRLA CILFSEF**WLPDSLALETPVRIFKSHHRKLKHR VVLLIVLGIRPSF*GIAFI*FWQDLTKRSSLIP TSDKISPLFLLY*SIVFCFGNTHSLCNSRAR*M QITLILCVTKLEKVFNKTC*FLY*Q*KCFYRY*C *QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QHA SLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*HW ASPWEKVALGKWSWRKQWELTKTSPRRRSPWP* RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*IF LEPAHRMGLSMS*LSMPLKATSENTSEPGGHPG WSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWST WLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPEI STI*TITKRPPMGGFQSSGWLQKPCLIEYTLIR VMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC* RKDTEWISQPTAPTNCT***GTVGMQCPPRDQRS SSW*KTWIEFSLSQPMRNTWTSANLSNSIHLVT LTQEVLVLQEMILFFLQTPCLTNHAFLSIHT*T AVLKHE*LCLPVPKQDSTGNLATLSRETMPPRAC CLHLYIWIRGVNNWKSNQHMCKDLYS*KLVIFP RRRRRFLEQWTATSHHVTPLTCRAYWLWTSRTQ GGRAFCLPC*FCNNWRRFMSAHTYRAQMQYIGAG CM*IYSNYV*IYIIYLQGVIFCIDFKWMSQCT* KIGLSFFNSYLLNAVLTHNFLIFTEQRWKNTFA FRENGITLIY**IGNIQNN*SFIVFFVI*VAFLC RQHSRLVNLLLGLN*LSDPLKREYLQYMTNLGK MKF*FICV*MLLSDDCS*TS*MPHIKRTHS*EG VSFWCATLSLRQRNV*LDFPR*MVPASS*KMP*S IP*GQTLVEMIAECASLWQLAFCF*VAH*SD*P VFSSVNFDNGFQTLWRWRRLLGSSSPIIEN*NT ELFC**FWGYVHLFKGLLSSNSGRTSPKDPASYL HQTKYRRCSFCTKVLCFALETPTHFAIAVQDEC RLH*SYVLQNWRKYLIKPVNFYTDNKNVSTDIN VNKTK*MSRNLFF*

Sequence FGFR2.aAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGAT AAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGAGGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAAC CAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCA TAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCA CAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACA CGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACAC TGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGT GAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAAT CTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCG GGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTA CTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGA GATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATC AGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCT GAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACG TTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTT ACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAA CGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGC TTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTC CCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTC AAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGC TGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACC TAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTG CTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCT ATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGG AAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAG GTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTA ATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAG CCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACA CAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATA CCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAA TGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTA ATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Multiple exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQALGD K*FQCRSAGSVQCDRGGCWGIYM*GLQLYRAGQ PVCLAHCPAKTASAWKRKGDYSFPRLPGDSHLLH RGLLNRLYGGNSHPVPNEEHDQEARLQQPAGCA QADQTYPPAETGNSFG*VQLLHELQHPAGEDNN TPLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADT GQAPGRRLLWASGHGGSSGN*QRQAQGGGHRGR EDVER*CHRERPF*SGVRDGDDEDDWETQEYHK SSWSLHTGWASLCHS*VCL*RQPPRIPPSPEATR DGVLL*H*PCS*GADDLQGLGVMHLPAGQRHGV LGFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQ RYQQYRLLQKDHQWAASSQVDGSRSPV**SIHSS E*CLVLRGVNVGDLHFRGLALPRDSRGGTF*AA EGRTQNG*ASQLHQRTVHDDEGLLACSALPETN VQAVGRRLGSNSHSHNQ*GILGPQPTSRTVFT*L P*HKKFLFFRR*FCFFSRPHALRTMPSSVSTHK RQC*NMNDCVCLSPNRTALGT*LH*AGRPCLPE LVVSTCIYGSEE*IIGKVISICVKIYTVENL*SS PGGEEGFWSSGLPQATM*PLSPAVRTGCGPVGL KVDVRSAFLVNFVIIGEDLCQHTLTEHKCSI*V LDVCKYIQIMYKYILYIYKELFFVLILNGCPNAP RKLVSLFLIAIC*MLFLHIIS*FSPSRGGKILL LSGKMV*R*FINKLVIYKTINHL*FFL*FKWHF YAGSTAD*LIYCLDLTSYQIL*KENIYNI*LIWG K*SFDLFVFKCCCQTIVLRPPKCPILKELIHRK VFHFGVQPCHYVNATSNWTSQDKWYQRPLKRCL NPFLEDRP*LK**QNVLLSGSWPSASELHINQIS LYSLQ*ILIMASRLFGVGDAC*DLQVPS*KIET QSCSADSFGDTSIFLRDCFHLILAGPHQKIQPH TYIRQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMN ADYTDLMCYKIGESI**NLLIFILTIKMFLQIL MLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGI NSSNAEVLALFNVTEADAGEYICKVSNYIGQAN QSAWLTVLPKQQAPGREKEITASPDYLEIAIYC IGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVH KLTKRIPLRRQVTVSAESSSSMNSNTPLVRITT RLSSTADTPMLAGVSEYELPEDPKWEFPRDKLT LGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAV KMLKDDATEKDLSDLVSEMEMMKMIGKHKNIIN LLGACTQDGPLYVIVEYASKGNLREYLRARRPP GMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEY LASQKCIHRDLAARNVLVTENNVMKIADFGLAR DINNIDYYKKTTNGRLPVKWMAPEALFDRVYTH QSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL KEGHRMDKPANCTNELYMMMRDCWHAVPSQRPT FKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPS YPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHIN GSVKT*MTVSACPQTGQHWEPSYTEQGDHASQS LLSPLVYMDQRSK*LEK*SAYV*RFIQLKTCNL PQEEKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DS RWTCVLPSLLIL**LEKIYVSTHLQSTNAVYRC WMYVNIFKLCINIYYIFTRSYFLY*F*MDVPMH LENWSLFF**LFAKCCSYT*FLNFHRAEVEKYFC FQGKWYNVNLLINW*YTKQLIIYSFFCNLSGIS MQAAQQTS*SIAWT*LVIRSFEKRIFTIYD*FG ENEVLIYLCLNAAVRRLFLDLLNAPY*KNSFIGR CFILVCNPVITSTQRLTGLPKINGTSVLLKDAL IHSLRTDLS*NDSRMCFSLAAGLLLLSCTLIRL ACILFSEF**WLPDSLALETPVRIFKSHHRKLKH RVVLLIVLGIRPSF*GIAFI*FWQDLTKRSSLI PTSDKISPLFLLY*SIVFCFGNTHSLCNSRAR* MQITLILCVTKLEKVFNKTC*FLY*Q*KCFYRY* C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSSTRG *IVPMQKCWLCSM*PRRMLGNIYVRSPII*GRPT SLPGSLSCQNSKRLEEKRRLQLPQTTWR*PFTA *GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLC TS*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QH ASLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*H WASPWEKVALGKWSWRKQWELTKTSPRRRSPWP *RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*I FLEPAHRMGLSMS*LSMPLKATSENTSEPGGHP GWSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWS TWLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPE ISTI*TITKRPPMGGFQSSGWLQKPCLIEYTLI RVMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC *RKDTEWISQPTAPTNCT***GTVGMQCPPRDQR SSSW*KTWIEFSLSQPMRNTWTSANLSNSIHLV TLTQEVLVLQEMILFFLQTPCLTNHAFLSIHT* TAVLKHE*LCLPVPKQDSTGNLATLSRETMPPRA CCLHLYIWIRGVNNWKSNQHMCKDLYS*KLVIF PRRRRRFLEQWTATSHHVTPLTCRAYWLWTSRT QGGRAFCLPC*FCNNWRRFMSAHTYRAQMQYIGA GCM*IYSNYV*IYIIYLQGVIFCIDFKWMSQCT *KIGLSFFNSYLLNAVLTHNFLIFTEQRWKNTF AFRENGITLIY**IGNIQNN*SFIVFFVI*VAFL CRQHSRLVNLLLGLN*LSDPLKREYLQYMTNLG KMKF*FICV*MLLSDDCS*TS*MPHIKRTHS*E GVSFWCATLSLRQRNV*LDFPR*MVPASS*KMP* SIP*GQTLVEMIAECASLWQLAFCF*VAH*SD* PVFSSVNFDNGFQTLWRWRRLLGSSSPIIEN*N TELFC**FWGYVHLFKGLLSSNSGRTSPKDPASY LHQTKYRRCSFCTKVLCFALETPTHFAIAVQDE CRLH*SYVLQNWRKYLIKPVNFYTDNKNVSTDI NVNKTK*MSRNLFF*