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Sequences

Sequence FGFR3.cAug05

CGCGCGCTGCCTGAGGACGCCGCGGCCCCCGCCCCCGCCATGGGCGCCCCTGCCTGCGCCCTCGCGCTCTGCGTGGCCGTGGCCATCGTGGCCGGCGCCT CCTCGGAGTCCTTGGGGACGGAGCAGCGCGTCGTGGGGCGAGCGGCAGAAGTCCCGGGCCCAGAGCCCGGCCAGCAGGAGCAGTTGGTCTTCGGCAGCGG GGATGCTGTGGAGCTGAGCTGTCCCCCGCCCGGGGGTGGTCCCATGGGGCCCACTGTCTGGGTCAAGGATGGCACAGGGCTGGTGCCCTCGGAGCGTGTC CTGGTGGGGCCCCAGCGGCTGCAGGTGCTGAATGCCTCCCACGAGGACTCCGGGGCCTACAGCTGCCGGCAGCGGCTCACGCAGCGCGTACTGTGCCACT TCAGTGTGCGGGTGACAGACGCTCCATCCTCGGGAGATGACGAAGACGGGGAGGACGAGGCTGAGGACACAGGTGTGGACACAGGGGCCCCTTACTGGAC ACGGCCCGAGCGGATGGACAAGAAGCTGCTGGCCGTGCCGGCCGCCAACACCGTCCGCTTCCGCTGCCCAGCCGCTGGCAACCCCACTCCCTCCATCTCC TGGCTGAAGAACGGCAGGGAGTTCCGCGGCGAGCACCGCATTGGAGGCATCAAGCTGCGGCATCAGCAGTGGAGCCTGGTCATGGAAAGCGTGGTGCCCT CGGACCGCGGCAACTACACCTGCGTCGTGGAGAACAAGTTTGGCAGCATCCGGCAGACGTACACGCTGGACGTGCTGGAGCGCTCCCCGCACCGGCCCAT CCTGCAGGCGGGGCTGCCGGCCAACCAGACGGCGGTGCTGGGCAGCGACGTGGAGTTCCACTGCAAGGTGTACAGTGACGCACAGCCCCACATCCAGTGG CTCAAGCACGTGGAGGTGAATGGCAGCAAGGTGGGCCCGGACGGCACACCCTACGTTACCGTGCTCAAGGTGTCCCTGGAGTCCAACGCGTCCATGAGCT CCAACACACCACTGGTGCGCATCGCAAGGCTGTCCTCAGGGGAGGGCCCCACGCTGGCCAATGTCTCCGAGCTCGAGCTGCCTGCCGACCCCAAATGGGA GCTGTCTCGGGCCCGGCTGACCCTGGGCAAGCCCCTTGGGGAGGGCTGCTTCGGCCAGGTGGTCATGGCGGAGGCCATCGGCATTGACAAGGACCGGGCC GCCAAGCCTGTCACCGTAGCCGTGAAGATGCTGAAAGACGATGCCACTGACAAGGACCTGTCGGACCTGGTGTCTGAGATGGAGATGATGAAGATGATCG GGAAACACAAAAACATCATCAACCTGCTGGGCGCCTGCACGCAGGGCGGGCCCCTGTACGTGCTGGTGGAGTACGCGGCCAAGGGTAACCTGCGGGAGTT TCTGCGGGCGCGGCGGCCCCCGGGCCTGGACTACTCCTTCGACACCTGCAAGCCGCCCGAGGAGCAGCTCACCTTCAAGGACCTGGTGTCCTGTGCCTAC CAGGTGGCCCGGGGCATGGAGTACTTGGCCTCCCAGAAGTGCATCCACAGGGACCTGGCTGCCCGCAATGTGCTGGTGACCGAGGACAACGTGATGAAGA TCGCAGACTTCGGGCTGGCCCGGGACGTGCACAACCTCGACTACTACAAGAAGACGACCAACGGCCGGCTGCCCGTGAAGTGGATGGCGCCTGAGGCCTT GTTTGACCGAGTCTACACTCACCAGAGTGACGTCTGGTCCTTTGGGGTCCTGCTCTGGGAGATCTTCACGCTGGGGGGCTCCCCGTACCCCGGCATCCCT GTGGAGGAGCTCTTCAAGCTGCTGAAGGAGGGCCACCGCATGGACAAGCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGCATG CCGCGCCCTCCCAGAGGCCCACCTTCAAGCAGCTGGTGGAGGACCTGGACCGTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCC TTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCAGCTCCAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGGCCCCACCC AGCAGTGGGGGCTCGCGGACGTGAAGGGCCACTGGTCCCCAACAATGTGAGGGGTCCCTAGCAGCCCACCCTGCTGCTGGTGCACAGCCACTCCCCGGCA TGAGACTCAGTGCAGATGGAGAGACAGCTACACAGAGCTTTGGTCTGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGCACATCCGCGTGTGCCTGT GTGCGTGCGCATCTTGCCTCCAGGTGCAGAGGTACCCTGGGTGTCCCCGCTGCTGTGCAACGGTCTCCTGACTGGTGCTGCAGCACCGAGGGGCCTTTGT TCTGGGGGGACCCAGTGCAGAATGTAAGTGGGCCCACCCGGTGGGACCCCCGTGGGGCAGGGAGCTGGGCCCGACATGGCTCCGGCCTCTGCCTTTGCAC CACGGGACATCACAGGGTGGGCCTCGGCCCCTCCCACACCCAAAGCTGAGCCTGCAGGGAAGCCCCACATGTCCAGCACCTTGTGCCTGGGGTGTTAGTG GCACCGCCTCCCCACCTCCAGGCTTTCCCACTTCCCACCCTGCCCCTCAGAGACTGAAATTACGGGTACCTGAAGATGGGAGCCTTTACCTTTTATGCAA AAGGTTTATTCCGGAAACTAGTGTACATTTCTATAAATAGATGCTGTGTATATGGTATATATACATATATATATATAACATATATGGAAGAGGAAAAGGC TGGTACAACGGAGGCCTGCGACCCTGGGGGCACAGGAGGCAGGCATGGCCCTGGGCGGGGCGTGGGGGGGCGTGGAGGGAGGCCCCAGGGGGTCTCACCC ATGCAAGCAGAGGACCAGGGCCTTTTCTGGCACCGCAGTTTTGTTTTAAAACTGGACCTGTATATTTGTAAAGCTATTTATGGGCCCCTGGCACTCTTGT TCCCACACCCCAACACTTCCAGCATTTAGCTGGCCACATGGCGGAGAGTTTTAATTTTTAACTTATTGACAACCGAGAAGGTTTATCCCGCCGATAGAGG GACGGCCAAGAATGTACGTCCAGCCTGCCCCGGAGCTGGAGGATCCCCTCCAAGCCTAAAAGGTTGTTAATAGTTGGAGGTGATTCCAGTGAAGATATTT TATTTCCTTTGTCCTTTTTCAGGAGAATTAGATTTCTATAGGATTTTTCTTTAGGAGATTTATTTTTTGGACTTCAAAGCAAGCTGGTATTTTCATACAA ATTCTTCTAATTGCTGTGTGTCCCAGGCAGGGAGACGGTTTCCAGGGAGGGGCCGGCCCTGTGTGCAGGTTCCGATGTTATTAGATGTTACAAGTTTATA TATATCTATATATATAATTTATTGAGTTTTTACAAGATGTATTTGTTGTAGACTTAACACTTCTTACGCAATGCTTCTAGAGTTTTATAGCCTGGACTGC TACCTTTCAAAGCTTGGAGGGAAGCCGTGAATTCAGTTGGTTCGTTCTGTACTGTTACTGGGCCCTGAGTCTGGGCAGCTGTCCCTTGCTTGCCTGCAGG GCCATGGCTCAGGGTGGTCTCTTCTTGGGGCCCAGTGCATGGTGGCCAGAGGTGTCACCCAAACCGGCAGGTGCGATTTTGTTAACCCAGCGACGAACTT TCCGAAAAATAAAGACACCTGGTTGCTAACCTGG

FGFR3.F3 (forward primer)
FGFR3.R3 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

RALPEDAAAPAPAMGAPACALALCVAVAIVAGA SSESLGTEQRVVGRAAEVPGPEPGQQEQLVFGSG DAVELSCPPPGGGPMGPTVWVKDGTGLVPSERV LVGPQRLQVLNASHEDSGAYSCRQRLTQRVLCH FSVRVTDAPSSGDDEDGEDEAEDTGVDTGAPYWT RPERMDKKLLAVPAANTVRFRCPAAGNPTPSIS WLKNGREFRGEHRIGGIKLRHQQWSLVMESVVP SDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPI LQAGLPANQTAVLGSDVEFHCKVYSDAQPHIQW LKHVEVNGSKVGPDGTPYVTVLKVSLESNASMS SNTPLVRIARLSSGEGPTLANVSELELPADPKWE LSRARLTLGKPLGEGCFGQVVMAEAIGIDKDRA AKPVTVAVKMLKDDATDKDLSDLVSEMEMMKMI GKHKNIINLLGACTQGGPLYVLVEYAAKGNLREF LRARRPPGLDYSFDTCKPPEEQLTFKDLVSCAY QVARGMEYLASQKCIHRDLAARNVLVTEDNVMK IADFGLARDVHNLDYYKKTTNGRLPVKWMAPEAL FDRVYTHQSDVWSFGVLLWEIFTLGGSPYPGIP VEELFKLLKEGHRMDKPANCTHDLYMIMRECWH AAPSQRPTFKQLVEDLDRVLTVTSTDEYLDLSAP FEQYSPGGQDTPSSSSSGDDSVFAHDLLPPAPP SSGGSRT*RATGPQQCEGSLAAHPAAGAQPLPG MRLSADGETATQSFGLCVCVCVCVCVCAHPRVPV CVRILPPGAEVPWVSPLLCNGLLTGAAAPRGLC SGGTQCRM*VGPPGGTPVGQGAGPDMAPASAFA PRDITGWASAPPTPKAEPAGKPHMSSTLCLGC*W HRLPTSRLSHFPPCPSETEITGT*RWEPLPFMQ KVYSGN*CTFL*IDAVYMVYIHIYI*HIWKRKR LVQRRPATLGAQEAGMALGGAWGGVEGGPRGSHP CKQRTRAFSGTAVLF*NWTCIFVKLFMGPWHSC SHTPTLPAFSWPHGGEF*FLTY*QPRRFIPPIE GRPRMYVQPAPELEDPLQA*KVVNSWR*FQ*RYF ISFVLFQEN*ISIGFFFRRFIFWTSKQAGIFIQ ILLIAVCPRQGDGFQGGAGPVCRFRCY*MLQVY IYLYI*FIEFLQDVFVVDLTLLTQCF*SFIAWTA TFQSLEGSREFSWFVLYCYWALSLGSCPLLACR AMAQGGLFLGPSAWWPEVSPKPAGAILLTQRRT FRKIKTPGC*P

Frame 1

ARCLRTPRPPPPPWAPLPAPSRSAWPWPSWPAP PRSPWGRSSASWGERQKSRAQSPASRSSWSSAA GMLWS*AVPRPGVVPWGPLSGSRMAQGWCPRSVS WWGPSGCRC*MPPTRTPGPTAAGSGSRSAYCAT SVCG*QTLHPREMTKTGRTRLRTQVWTQGPLTG HGPSGWTRSCWPCRPPTPSASAAQPLATPLPPSP G*RTAGSSAASTALEASSCGISSGAWSWKAWCP RTAATTPASWRTSLAASGRRTRWTCWSAPRTGP SCRRGCRPTRRRCWAATWSSTARCTVTHSPTSSG SSTWR*MAARWARTAHPTLPCSRCPWSPTRP*A PTHHWCASQGCPQGRAPRWPMSPSSSCLPTPNG SCLGPG*PWASPLGRAASARWSWRRPSALTRTGP PSLSP*P*RC*KTMPLTRTCRTWCLRWR**R*S GNTKTSSTCWAPARRAGPCTCWWSTRPRVTCGS FCGRGGPRAWTTPSTPASRPRSSSPSRTWCPVPT RWPGAWSTWPPRSASTGTWLPAMCW*PRTT**R SQTSGWPGTCTTSTTTRRRPTAGCP*SGWRLRP CLTESTLTRVTSGPLGSCSGRSSRWGAPRTPASL WRSSSSC*RRATAWTSPPTAHTTCT*SCGSAGM PRPPRGPPSSSWWRTWTVSLP*RPPTSTWTCRR LSSSTPRVARTPPAPAPQGTTPCLPTTCCPRPHP AVGARGREGPLVPNNVRGP*QPTLLLVHSHSPA *DSVQMERQLHRALVCVCVCACVCVCVHIRVCL CACASCLQVQRYPGCPRCCATVS*LVLQHRGAFV LGGPSAECKWAHPVGPPWGRELGPTWLRPLPLH HGTSQGGPRPLPHPKLSLQGSPTCPAPCAWGVS GTASPPPGFPTSHPAPQRLKLRVPEDGSLYLLCK RFIPETSVHFYK*MLCIWYIYIYIYNIYGRGKG WYNGGLRPWGHRRQAWPWAGRGGAWREAPGGLT HASRGPGPFLAPQFCFKTGPVYL*SYLWAPGTLV PTPQHFQHLAGHMAESFNF*LIDNREGLSRR*R DGQECTSSLPRSWRIPSKPKRLLIVGGDSSEDI LFPLSFFRRIRFL*DFSLGDLFFGLQSKLVFSYK FF*LLCVPGRETVSREGPALCAGSDVIRCYKFI YIYIYNLLSFYKMYLL*T*HFLRNASRVL*PGL LPFKAWREAVNSVGSFCTVTGP*VWAAVPCLPAG PWLRVVSSWGPVHGGQRCHPNRQVRFC*PSDEL SEK*RHLVANL

Frame 2

RAA*GRRGPRPRHGRPCLRPRALRGRGHRGRRL LGVLGDGAARRGASGRSPGPRARPAGAVGLRQR GCCGAELSPARGWSHGAHCLGQGWHRAGALGAC PGGAPAAAGAECLPRGLRGLQLPAAAHAARTVPL QCAGDRRSILGR*RRRGGRG*GHRCGHRGPLLD TARADGQEAAGRAGRQHRPLPLPSRWQPHSLHL LAEERQGVPRRAPHWRHQAAASAVEPGHGKRGAL GPRQLHLRRGEQVWQHPADVHAGRAGALPAPAH PAGGAAGQPDGGAGQRRGVPLQGVQ*RTAPHPV AQARGGEWQQGGPGRHTLRYRAQGVPGVQRVHEL QHTTGAHRKAVLRGGPHAGQCLRARAACRPQMG AVSGPADPGQAPWGGLLRPGGHGGGHRH*QGPG RQACHRSREDAERRCH*QGPVGPGV*DGDDEDDR ETQKHHQPAGRLHAGRAPVRAGGVRGQG*PAGV SAGAAAPGPGLLLRHLQAARGAAHLQGPGVLCL PGGPGHGVLGLPEVHPQGPGCPQCAGDRGQRDED RRLRAGPGRAQPRLLQEDDQRPAAREVDGA*GL V*PSLHSPE*RLVLWGPALGDLHAGGLPVPRHP CGGALQAAEGGPPHGQARQLHTRPVHDHAGVLAC RALPEAHLQAAGGGPGPCPYRDVHRRVPGPVGA FRAVLPGWPGHPQLQLLRGRLRVCPRPAAPGPT QQWGLADVKGHWSPTM*GVPSSPPCCWCTATPRH ETQCRWRDSYTELWSVCVCVRVCVCVCTSACAC VRAHLASRCRGTLGVPAAVQRSPDWCCSTEGPL FWGDPVQNVSGPTRWDPRGAGSWARHGSGLCLCT TGHHRVGLGPSHTQS*ACREAPHVQHLVPGVLV APPPHLQAFPLPTLPLRD*NYGYLKMGAFTFYA KGLFRKLVYISINRCCVYGIYTYIYITYMEEEKA GTTEACDPGGTGGRHGPGRGVGGRGGRPQGVSP MQAEDQGLFWHRSFVLKLDLYICKAIYGPLALL FPHPNTSSI*LATWRRVLIFNLLTTEKVYPADRG TAKNVRPACPGAGGSPPSLKGC**LEVIPVKIF YFLCPFSGELDFYRIFL*EIYFLDFKASWYFHT NSSNCCVSQAGRRFPGRGRPCVQVPMLLDVTSLY ISIYIIY*VFTRCICCRLNTSYAMLLEFYSLDC YLSKLGGKP*IQLVRSVLLLGPESGQLSLACLQ GHGSGWSLLGAQCMVARGVTQTGRCDFVNPATNF PKNKDTWLLTW

Sequence FGFR3.aAug05

GCGGCGTCGCGGGCAGCTGGCGCCGCGCGGTCCTGCTCTGCCGGTCGCACGGACGCACCGGCGGGCCGCCGGCCGGAGGGACGGGGCGGGAGCTGGGCCC GCGGACAGCGAGCCGGAGCGGGAGCCGCGCGTAGCGAGCCGGGCTCCGGCGCTCGCCAGTCTCCCGAGCGGCGCCCGCCTCCCGCCGGTGCCCGCGCCGG GCCGTGGGGGGCAGCATGCCCGCGCGCGCTGCCTGAGGACGCCGCGGCCCCCGCCCCCGCCATGGGCGCCCCTGCCTGCGCCCTCGCGCTCTGCGTGGCC GTGGCCATCGTGGCCGGCGCCTCCTCGGAGTCCTTGGGGACGGAGCAGCGCGTCGTGGGGCGAGCGGCAGAAGTCCCGGGCCCAGAGCCCGGCCAGCAGG AGCAGTTGGTCTTCGGCAGCGGGGATGCTGTGGAGCTGAGCTGTCCCCCGCCCGGGGGTGGTCCCATGGGGCCCACTGTCTGGGTCAAGGATGGCACAGG GCTGGTGCCCTCGGAGCGTGTCCTGGTGGGGCCCCAGCGGCTGCAGGTGCTGAATGCCTCCCACGAGGACTCCGGGGCCTACAGCTGCCGGCAGCGGCTC ACGCAGCGCGTACTGTGCCACTTCAGTGTGCGGGTGACAGACGCTCCATCCTCGGGAGATGACGAAGACGGGGAGGACGAGGCTGAGGACACAGGTGTGG ACACAGGGGCCCCTTACTGGACACGGCCCGAGCGGATGGACAAGAAGCTGCTGGCCGTGCCGGCCGCCAACACCGTCCGCTTCCGCTGCCCAGCCGCTGG CAACCCCACTCCCTCCATCTCCTGGCTGAAGAACGGCAGGGAGTTCCGCGGCGAGCACCGCATTGGAGGCATCAAGCTGCGGCATCAGCAGTGGAGCCTG GTCATGGAAAGCGTGGTGCCCTCGGACCGCGGCAACTACACCTGCGTCGTGGAGAACAAGTTTGGCAGCATCCGGCAGACGTACACGCTGGACGTGCTGG AGCGCTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTGCCGGCCAACCAGACGGCGGTGCTGGGCAGCGACGTGGAGTTCCACTGCAAGGTGTACAGTGA CGCACAGCCCCACATCCAGTGGCTCAAGCACGTGGAGGTGAATGGCAGCAAGGTGGGCCCGGACGGCACACCCTACGTTACCGTGCTCAAGACGGCGGGC GCTAACACCACCGACAAGGAGCTAGAGGTTCTCTCCTTGCACAACGTCACCTTTGAGGACGCCGGGGAGTACACCTGCCTGGCGGGCAATTCTATTGGGT TTTCTCATCACTCTGCGTGGCTGGTGGTGCTGCCAGCCGAGGAGGAGCTGGTGGAGGCTGACGAGGCGGGCAGTGTGTATGCAGGCATCCTCAGCTACGG GGTGGGCTTCTTCCTGTTCATCCTGGTGGTGGCGGCTGTGACGCTCTGCCGCCTGCGCAGCCCCCCCAAGAAAGGCCTGGGCTCCCCCACCGTGCACAAG ATCTCCCGCTTCCCGCTCAAGCGACAGCAGGTGTCCCTGGAGTCCAACGCGTCCATGAGCTCCAACACACCACTGGTGCGCATCGCAAGGCTGTCCTCAG GGGAGGGCCCCACGCTGGCCAATGTCTCCGAGCTCGAGCTGCCTGCCGACCCCAAATGGGAGCTGTCTCGGGCCCGGCTGACCCTGGGCAAGCCCCTTGG GGAGGGCTGCTTCGGCCAGGTGGTCATGGCGGAGGCCATCGGCATTGACAAGGACCGGGCCGCCAAGCCTGTCACCGTAGCCGTGAAGATGCTGAAAGAC GATGCCACTGACAAGGACCTGTCGGACCTGGTGTCTGAGATGGAGATGATGAAGATGATCGGGAAACACAAAAACATCATCAACCTGCTGGGCGCCTGCA CGCAGGGCGGGCCCCTGTACGTGCTGGTGGAGTACGCGGCCAAGGGTAACCTGCGGGAGTTTCTGCGGGCGCGGCGGCCCCCGGGCCTGGACTACTCCTT CGACACCTGCAAGCCGCCCGAGGAGCAGCTCACCTTCAAGGACCTGGTGTCCTGTGCCTACCAGGTGGCCCGGGGCATGGAGTACTTGGCCTCCCAGAAG TGCATCCACAGGGACCTGGCTGCCCGCAATGTGCTGGTGACCGAGGACAACGTGATGAAGATCGCAGACTTCGGGCTGGCCCGGGACGTGCACAACCTCG ACTACTACAAGAAGACGACCAACCTGGTCCTTTGGGGTCCTGCTCTGGGAGATCTTCACGCTGGGGGGCTCCCCGTACCCCGGCATCCCTGTGGAGGAGC TCTTCAAGCTGCTGAAGGAGGGCCACCGCATGGACAAGCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGCATGCCGCGCCCTC CCAGAGGCCCACCTTCAAGCAGCTGGTGGAGGACCTGGACCGTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCTTTCGAGCAG TACTCCCCGGGTGGCCAGGACACCCCCAGCTCCAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGGCCCCACCCAGCAGTGGGG GCTCGCGGACGTGAAGGGCCACTGGTCCCCAACAATGTGAGGGGTCCCTAGCAGCCCACCCTGCTGCTGGTGCACAGCCACTCCCCGGCATGAGACTCAG TGCAGATGGAGAGACAGCTACACAGAGCTTTGGTCTGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGCACATCCGCGTGTGCCTGTGTGCGTGCGC ATCTTGCCTCCAGGTGCAGAGGTACCCTGGGTGTCCCCGCTGCTGTGCAACGGTCTCCTGACTGGTGCTGCAGCACCGAGGGGCCTTTGTTCTGGGGGGA CCCAGTGCAGAATGTAAGTGGGCCCACCCGGTGGGACCCCCGTGGGGCAGGGAGCTGGGCCCGACATGGCTCCGGCCTCTGCCTTTGCACCACGGGACAT CACAGGGTGGGCCTCGGCCCCTCCCACACCCAAAGCTGAGCCTGCAGGGAAGCCCCACATGTCCAGCACCTTGTGCCTGGGGTGTTAGTGGCACCGCCTC CCCACCTCCAGGCTTTCCCACTTCCCACCCTGCCCCTCAGAGACTGAAATTACGGGTACCTGAAGATGGGAGCCTTTACCTTTTATGCAAAAGGTTTATT CCGGAAACTAGTGTACATTTCTATAAATAGATGCTGTGTATATGGTATATATACATATATATATATAACATATATGGAAGAGGAAAAGGCTGGTACAACG GAGGCCTGCGACCCTGGGGGCACAGGAGGCAGGCATGGCCCTGGGCGGGGCGTGGGGGGGCGTGGAGGGAGGCCCCAGGGGGTCTCACCCATGCAAGCAG AGGACCAGGGCCTTTTCTGGCACCGCAGTTTTGTTTTAAAACTGGACCTGTATATTTGTAAAGCTATTTATGGGCCCCTGGCACTCTTGTTCCCACACCC CAACACTTCCAGCATTTAGCTGGCCACATGGCGGAGAGTTTTAATTTTTAACTTATTGACAACCGAGAAGGTTTATCCCGCCGATAGAGGGACGGCCAAG AATGTACGTCCAGCCTGCCCCGGAGCTGGAGGATCCCCTCCAAGCCTAAAAGGTTGTTAATAGTTGGAGGTGATTCCAGTGAAGATATTTTATTTCCTTT GTCCTTTTTCAGGAGAATTAGATTTCTATAGGATTTTTCTTTAGGAGATTTATTTTTTGGACTTCAAAGCAAGCTGGTATTTTCATACAAATTCTTCTAA TTGCTGTGTGTCCCAGGCAGGGAGACGGTTTCCAGGGAGGGGCCGGCCCTGTGTGCAGGTTCCGATGTTATTAGATGTTACAAGTTTATATATATCTATA TATATAATTTATTGAGTTTTTACAAGATGTATTTGTTGTAGACTTAACACTTCTTACGCAATGCTTCTAGAGTTTTATAGCCTGGACTGCTACCTTTCAA AGCTTGGAGGGAAGCCGTGAATTCAGTTGGTTCGTTCTGTACTGTTACTGGGCCCTGAGTCTGGGCAGCTGTCCCTTGCTTGCCTGCAGGGCCATGGCTC AGGGTGGTCTCTTCTTGGGGCCCAGTGCATGGTGGCCAGAGGTGTCACCCAAACCGGCAGGTGCGATTTTGTTAACCCAGCGACGAACTTTCCGAAAAAT AAAGACACCTGGTTGCTAACCTGG

FGFR3.F3 (forward primer)
FGFR3.R3 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AASRAAGAARSCSAGRTDAPAGRRPEGRGGSWA RGQRAGAGAARSEPGSGARQSPERRPPPAGARAG PWGAACPRALPEDAAAPAPAMGAPACALALCVA VAIVAGASSESLGTEQRVVGRAAEVPGPEPGQQ EQLVFGSGDAVELSCPPPGGGPMGPTVWVKDGTG LVPSERVLVGPQRLQVLNASHEDSGAYSCRQRL TQRVLCHFSVRVTDAPSSGDDEDGEDEAEDTGV DTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAG NPTPSISWLKNGREFRGEHRIGGIKLRHQQWSL VMESVVPSDRGNYTCVVENKFGSIRQTYTLDVL ERSPHRPILQAGLPANQTAVLGSDVEFHCKVYSD AQPHIQWLKHVEVNGSKVGPDGTPYVTVLKTAG ANTTDKELEVLSLHNVTFEDAGEYTCLAGNSIG FSHHSAWLVVLPAEEELVEADEAGSVYAGILSYG VGFFLFILVVAAVTLCRLRSPPKKGLGSPTVHK ISRFPLKRQQVSLESNASMSSNTPLVRIARLSS GEGPTLANVSELELPADPKWELSRARLTLGKPLG EGCFGQVVMAEAIGIDKDRAAKPVTVAVKMLKD DATDKDLSDLVSEMEMMKMIGKHKNIINLLGAC TQGGPLYVLVEYAAKGNLREFLRARRPPGLDYSF DTCKPPEEQLTFKDLVSCAYQVARGMEYLASQK CIHRDLAARNVLVTEDNVMKIADFGLARDVHNL DYYKKTTNLVLWGPALGDLHAGGLPVPRHPCGGA LQAAEGGPPHGQARQLHTRPVHDHAGVLACRAL PEAHLQAAGGGPGPCPYRDVHRRVPGPVGAFRA VLPGWPGHPQLQLLRGRLRVCPRPAAPGPTQQWG LADVKGHWSPTM*GVPSSPPCCWCTATPRHETQ CRWRDSYTELWSVCVCVRVCVCVCTSACACVRA HLASRCRGTLGVPAAVQRSPDWCCSTEGPLFWGD PVQNVSGPTRWDPRGAGSWARHGSGLCLCTTGH HRVGLGPSHTQS*ACREAPHVQHLVPGVLVAPP PHLQAFPLPTLPLRD*NYGYLKMGAFTFYAKGLF RKLVYISINRCCVYGIYTYIYITYMEEEKAGTT EACDPGGTGGRHGPGRGVGGRGGRPQGVSPMQA EDQGLFWHRSFVLKLDLYICKAIYGPLALLFPHP NTSSI*LATWRRVLIFNLLTTEKVYPADRGTAK NVRPACPGAGGSPPSLKGC**LEVIPVKIFYFL CPFSGELDFYRIFL*EIYFLDFKASWYFHTNSSN CCVSQAGRRFPGRGRPCVQVPMLLDVTSLYISI YIIY*VFTRCICCRLNTSYAMLLEFYSLDCYLS KLGGKP*IQLVRSVLLLGPESGQLSLACLQGHGS GWSLLGAQCMVARGVTQTGRCDFVNPATNFPKN KDTWLLTW

Frame 1

RRRGQLAPRGPALPVARTHRRAAGRRDGAGAGP ADSEPEREPRVASRAPALASLPSGARLPPVPAP GRGGQHARARCLRTPRPPPPPWAPLPAPSRSAWP WPSWPAPPRSPWGRSSASWGERQKSRAQSPASR SSWSSAAGMLWS*AVPRPGVVPWGPLSGSRMAQ GWCPRSVSWWGPSGCRC*MPPTRTPGPTAAGSGS RSAYCATSVCG*QTLHPREMTKTGRTRLRTQVW TQGPLTGHGPSGWTRSCWPCRPPTPSASAAQPL ATPLPPSPG*RTAGSSAASTALEASSCGISSGAW SWKAWCPRTAATTPASWRTSLAASGRRTRWTCW SAPRTGPSCRRGCRPTRRRCWAATWSSTARCTV THSPTSSGSSTWR*MAARWARTAHPTLPCSRRRA LTPPTRS*RFSPCTTSPLRTPGSTPAWRAILLG FLITLRGWWCCQPRRSWWRLTRRAVCMQASSAT GWASSCSSWWWRL*RSAACAAPPRKAWAPPPCTR SPASRSSDSRCPWSPTRP*APTHHWCASQGCPQ GRAPRWPMSPSSSCLPTPNGSCLGPG*PWASPL GRAASARWSWRRPSALTRTGPPSLSP*P*RC*KT MPLTRTCRTWCLRWR**R*SGNTKTSSTCWAPA RRAGPCTCWWSTRPRVTCGSFCGRGGPRAWTTP STPASRPRSSSPSRTWCPVPTRWPGAWSTWPPRS ASTGTWLPAMCW*PRTT**RSQTSGWPGTCTTS TTTRRRPTWSFGVLLWEIFTLGGSPYPGIPVEE LFKLLKEGHRMDKPANCTHDLYMIMRECWHAAPS QRPTFKQLVEDLDRVLTVTSTDEYLDLSAPFEQ YSPGGQDTPSSSSSGDDSVFAHDLLPPAPPSSG GSRT*RATGPQQCEGSLAAHPAAGAQPLPGMRLS ADGETATQSFGLCVCVCVCVCVCAHPRVPVCVR ILPPGAEVPWVSPLLCNGLLTGAAAPRGLCSGG TQCRM*VGPPGGTPVGQGAGPDMAPASAFAPRDI TGWASAPPTPKAEPAGKPHMSSTLCLGC*WHRL PTSRLSHFPPCPSETEITGT*RWEPLPFMQKVY SGN*CTFL*IDAVYMVYIHIYI*HIWKRKRLVQR RPATLGAQEAGMALGGAWGGVEGGPRGSHPCKQ RTRAFSGTAVLF*NWTCIFVKLFMGPWHSCSHT PTLPAFSWPHGGEF*FLTY*QPRRFIPPIEGRPR MYVQPAPELEDPLQA*KVVNSWR*FQ*RYFISF VLFQEN*ISIGFFFRRFIFWTSKQAGIFIQILL IAVCPRQGDGFQGGAGPVCRFRCY*MLQVYIYLY I*FIEFLQDVFVVDLTLLTQCF*SFIAWTATFQ SLEGSREFSWFVLYCYWALSLGSCPLLACRAMA QGGLFLGPSAWWPEVSPKPAGAILLTQRRTFRKI KTPGC*P

Frame 2

GVAGSWRRAVLLCRSHGRTGGPPAGGTGRELGP RTASRSGSRA*RAGLRRSPVSRAAPASRRCPRR AVGGSMPARAA*GRRGPRPRHGRPCLRPRALRG RGHRGRRLLGVLGDGAARRGASGRSPGPRARPAG AVGLRQRGCCGAELSPARGWSHGAHCLGQGWHR AGALGACPGGAPAAAGAECLPRGLRGLQLPAAA HAARTVPLQCAGDRRSILGR*RRRGGRG*GHRCG HRGPLLDTARADGQEAAGRAGRQHRPLPLPSRW QPHSLHLLAEERQGVPRRAPHWRHQAAASAVEP GHGKRGALGPRQLHLRRGEQVWQHPADVHAGRAG ALPAPAHPAGGAAGQPDGGAGQRRGVPLQGVQ* RTAPHPVAQARGGEWQQGGPGRHTLRYRAQDGG R*HHRQGARGSLLAQRHL*GRRGVHLPGGQFYWV FSSLCVAGGAASRGGAGGG*RGGQCVCRHPQLR GGLLPVHPGGGGCDALPPAQPPQERPGLPHRAQ DLPLPAQATAGVPGVQRVHELQHTTGAHRKAVLR GGPHAGQCLRARAACRPQMGAVSGPADPGQAPW GGLLRPGGHGGGHRH*QGPGRQACHRSREDAER RCH*QGPVGPGV*DGDDEDDRETQKHHQPAGRLH AGRAPVRAGGVRGQG*PAGVSAGAAAPGPGLLL RHLQAARGAAHLQGPGVLCLPGGPGHGVLGLPE VHPQGPGCPQCAGDRGQRDEDRRLRAGPGRAQPR LLQEDDQPGPLGSCSGRSSRWGAPRTPASLWRS SSSC*RRATAWTSPPTAHTTCT*SCGSAGMPRP PRGPPSSSWWRTWTVSLP*RPPTSTWTCRRLSSS TPRVARTPPAPAPQGTTPCLPTTCCPRPHPAVG ARGREGPLVPNNVRGP*QPTLLLVHSHSPA*DS VQMERQLHRALVCVCVCACVCVCVHIRVCLCACA SCLQVQRYPGCPRCCATVS*LVLQHRGAFVLGG PSAECKWAHPVGPPWGRELGPTWLRPLPLHHGT SQGGPRPLPHPKLSLQGSPTCPAPCAWGVSGTAS PPPGFPTSHPAPQRLKLRVPEDGSLYLLCKRFI PETSVHFYK*MLCIWYIYIYIYNIYGRGKGWYN GGLRPWGHRRQAWPWAGRGGAWREAPGGLTHASR GPGPFLAPQFCFKTGPVYL*SYLWAPGTLVPTP QHFQHLAGHMAESFNF*LIDNREGLSRR*RDGQ ECTSSLPRSWRIPSKPKRLLIVGGDSSEDILFPL SFFRRIRFL*DFSLGDLFFGLQSKLVFSYKFF* LLCVPGRETVSREGPALCAGSDVIRCYKFIYIY IYNLLSFYKMYLL*T*HFLRNASRVL*PGLLPFK AWREAVNSVGSFCTVTGP*VWAAVPCLPAGPWL RVVSSWGPVHGGQRCHPNRQVRFC*PSDELSEK *RHLVANL