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Sequences

Sequence GLI1.bAug05

CCCAGACTCCAGCCCTGGACCGCGCATCCCGAGCCCAGCGCCCAGACAGAGGCCCACTCTTTTCTTCTCCCCGGAGTGCAGTCAAGTTGACCAAGAAGCG
GGCACTGTCCATCTCACCTCTGTCGGATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAGCTTTCATCAACTCGCGATGC
ACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCACCATGAGCCCATCTCTGGGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTT
CCTTTGGGGTCCAGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATCCTCAGTCCCGGGGACCCTTCCCAACTTGCCAGCTGAAGTC
TGAGCTGGACATGCTGGTTGGCAAGTGCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCCAACTCCACAGGCATACAGGATCCCCTGTTGGGGATG
CTGGATGGGCGGGAGGACCTCGAGAGAGAGGAGAAGCGTGAGCCTGAATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGAATTTGACT
CCCAAGAGCAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGGGAGCGGAAGGAGTTCGTGTGCCACTGGGGGGGCTGCTCCAGGGAGCTGAGGCC
CTTCAAAGCCCAGTACATGCTGGTGGTTCACATGCGCAGACACACTGGCGAGAAGCCACACAAGTGCACG
  • GLI1.F1 (forward primer)
  • GLI1.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

PRLQPWTAHPEPSAQTEAHSFLLPGVQSS*PRS
GHCPSHLCRMPAWTCRRLSAPHPAPS*LSSTRDA
HLQEAPTVISPLAP*AHLWDSQPR*ITKKGPRL
PLGSSLVVPMTLPGVG*SHILSPGDPSQLAS*S
LSWTCWLASAGRNPWKVICPAPTPQAYRIPCWGC
WMGGRTSRERRSVSLNLCMKLTAVGMAAARNLT
PKSSWCTTSTASTSTGSGRSSCATGGAAPGS*G
PSKPSTCWWFTCADTLARSHTSA

Frame 1

PDSSPGPRIPSPAPRQRPTLFFSPECSQVDQEA
GTVHLTSVGCQPGPADGYPHLTQLPRSFHQLAM
HISRRLLRSSLHWHHEPISGIPSPDESPKRALAF
LWGPALWSP*LCPGWDDPTSSVPGTLPNLPAEV
*AGHAGWQVPGGTLGR*YVQPQLHRHTGSPVGD
AGWAGGPRERGEA*A*ICV*N*LPLGWLQPGI*L
PRAAGAPHQQRAHPRGAEGVRVPLGGLLQGAEA
LQSPVHAGGSHAQTHWREATQVH

Frame 2

QTPALDRASRAQRPDRGPLFSSPRSAVKLTKKR
ALSISPLSDASLDLQTVIRTSPSSLVAFINSRC
TSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSP
SFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKS
ELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGM
LDGREDLEREEKREPESVYETDCRWDGCSQEFD
SQEQLVHHINSEHIHGERKEFVCHWGGCSRELRP
FKAQYMLVVHMRRHTGEKPHKCT

Sequence GLI1.aAug05

CCCAGACTCCAGCCCTGGACCGCGCATCCCGAGCCCAGCGCCCAGACAGAGTGTCCCCACACCCTCCTCTGAGACGCCATGTTCAACTCGATGACCCCAC
CACCAATCAGTAGCTATGGCGAGCCCTGCTGTCTCCGGCCCCTCCCCAGTCAGGGGGCCCCCAGTGTGGGGACAGAAGGACTGTCTGGCCCGCCCTTCTG
CCACCAAGCTAACCTCATGTCCGGCCCCCACAGTTATGGGCCAGCCAGAGAGACCAACAGCTGCACCGAGGGCCCACTCTTTTCTTCTCCCCGGAGTGCA
GTCAAGTTGACCAAGAAGCGGGCACTGTCCATCTCACCTCTGTCGGATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAG
CTTTCATCAACTCGCGATGCACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCACCATGAGCCCATCTCTGGGATTCCCAGCCCAGATGAATCA
CCAAAAAGGGCCCTCGCCTTCCTTTGGGGTCCAGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATCCTCAGTCCCGGGGACCCTTC
CCAACTTGCCAGCTGAAGTCTGAGCTGGACATGCTGGTTGGCAAGTGCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCCAACTCCACAGGCATAC
AGGATCCCCTGTTGGGGATGCTGGATGGGCGGGAGGACCTCGAGAGAGAGGAGAAGCGTGAGCCTGAATCTGTGTATGAAACTGACTGCCGTTGGGATGG
CTGCAGCCAGGAATTTGACTCCCAAGAGCAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGGGAGCGGAAGGAGTTCGTGTGCCACTGGGGGGGC
TGCTCCAGGGAGCTGAGGCCCTTCAAAGCCCAGTACATGCTGGTGGTTCACATGCGCAGACACACTGGCGAGAAGCCACACAAGTGCACGTTTGAAGGGT
GCCGGAAGTCATACTCACGCCTCGAAAACCTGAAGACGCACCTGCGGTCACACACGGGTGAGAAGCCATACATGTGTGAGCACGAGGGCTGCAGTAAAGC
CTTCAGCAATGCCAGTGACCGAGCCAAGCACCAGAATCGGACCCATTCCAATGAGAAGCCGTATGTATGTAAGCTCCCTGGCTGCACCAAACGCTATACA
GATCCTAGCTCGCTGCGAAAACATGTCAAGACAGTGCATGGTCCTGACGCCCATGTGACCAAACGGCACCGTGGGGATGGCCCCCTGCCTCGGGCACCAT
CCATTTCTACAGTGGAGCCCAAGAGGGAGCGGGAAGGAGGTCCCATCAGGGAGGAAAGCAGACTGACTGTGCCAGAGGGTGCCATGAAGCCACAGCCAAG
CCCTGGGGCCCAGTCATCCTGCAGCAGTGACCACTCCCCGGCAGGGAGTGCAGCCAATACAGACAGTGGTGTGGAAATGACTGGCAATGCAGGGGGCAGC
ACTGAAGACCTCTCCAGCTTGGACGAGGGACCTTGCATTGCTGGCACTGGTCTGTCCACTCTTCGCCGCCTTGAGAACCTCAGGCTGGACCAGCTACATC
AACTCCGGCCAATAGGGACCCGGGGTCTCAAACTGCCCAGCTTGTCCCACACCGGTACCACTGTGTCCCGCCGCGTGGGCCCCCCAGTCTCTCTTGAACG
CCGCAGCAGCAGCTCCAGCAGCATCAGCTCTGCCTATACTGTCAGCCGCCGCTCCTCCCTGGCCTCTCCTTTCCCCCCTGGCTCCCCACCAGAGAATGGA
GCATCCTCCCTGCCTGGCCTTATGCCTGCCCAGCACTACCTGCTTCGGGCAAGATATGCTTCAGCCAGAGGGGGTGGTACTTCGCCCACTGCAGCATCCA
GCCTGGATCGGATAGGTGGTCTTCCCATGCCTCCTTGGAGAAGCCGAGCCGAGTATCCAGGATACAACCCCAATGCAGGGGTCACCCGGAGGGCCAGTGA
CCCAGCCCAGGCTGCTGACCGTCCTGCTCCAGCTAGAGTCCAGAGGTTCAAGAGCCTGGGCTGTGTCCATACCCCACCCACTGTGGCAGGGGGAGGACAG
AACTTTGATCCTTACCTCCCAACCTCTGTCTACTCACCACAGCCCCCCAGCATCACTGAGAATGCTGCCATGGATGCTAGAGGGCTACAGGAAGAGCCAG
AAGTTGGGACCTCCATGGTGGGCAGTGGTCTGAACCCCTATATGGACTTCCCACCTACTGATACTCTGGGATATGGGGGACCTGAAGGGGCAGCAGCTGA
GCCTTATGGAGCGAGGGGTCCAGGCTCTCTGCCTCTTGGGCCTGGTCCACCCACCAACTATGGCCCCAACCCCTGTCCCCAGCAGGCCTCATATCCTGAC
CCCACCCAAGAAACATGGGGTGAGTTCCCTTCCCACTCTGGGCTGTACCCAGGCCCCAAGGCTCTAGGTGGAACCTACAGCCAGTGTCCTCGACTTGAAC
ATTATGGACAAGTGCAAGTCAAGCCAGAACAGGGGTGCCCAGTGGGGTCTGACTCCACAGGACTGGCACCCTGCCTCAATGCCCACCCCAGTGAGGGGCC
CCCACATCCACAGCCTCTCTTTTCCCATTACCCCCAGCCCTCTCCTCCCCAATATCTCCAGTCAGGCCCCTATACCCAGCCACCCCCTGATTATCTTCCT
TCAGAACCCAGGCCTTGCCTGGACTTTGATTCCCCCACCCATTCCACAGGGCAGCTCAAGGCTCAGCTTGTGTGTAATTATGTTCAATCTCAACAGGAGC
TACTGTGGGAGGGTGGGGGCAGGGAAGATGCCCCCGCCCAGGAACCTTCCTACCAGAGTCCCAAGTTTCTGGGGGGTTCCCAGGTTAGCCCAAGCCGTGC
TAAAGCTCCAGTGAACACATATGGACCTGGCTTTGGACCCAACTTGCCCAATCACAAGTCAGGTTCCTATCCCACCCCTTCACCATGCCATGAAAATTTT
GTAGTGGGGGCAAATAGGGCTTCACATAGGGCAGCAGCACCACCTCGACTTCTGCCCCCATTGCCCACTTGCTATGGGCCTCTCAAAGTGGGAGGCACAA
ACCCCAGCTGTGGTCATCCTGAGGTGGGCAGGCTAGGAGGGGGTCCTGCCTTGTACCCTCCTCCCGAAGGACAGGTATGTAACCCCCTGGACTCTCTTGA
TCTTGACAACACTCAGCTGGACTTTGTGGCTATTCTGGATGAGCCCCAGGGGCTGAGTCCTCCTCCTTCCCATGATCAGCGGGGCAGCTCTGGACATACC
CCACCTCCCTCTGGGCCCCCCAACATGGCTGTGGGCAACATGAGTGTCTTACTGAGATCCCTACCTGGGGAAACAGAATTCCTCAACTCTAGTGCCTAAA
GAGTAGGGAATCTCATCCATCACAGATCGCATTTCCTAAGGGGTTTCTATCCTTCCAGAAAAATTGGGGGAGCTGCAGTCCCATGCACAAGATGCCCCAG
GGATGGGAGGTATGGGCTGGGGGCTATGTATAGTCTGTATACGTTTTGAGGAGAAATTTGATAATGACACTGTTTCCTGATAATAAAGGAACTGCATCAG
AAAAA
  • GLI1.F1 (forward primer)
  • GLI1.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

PRLQPWTAHPEPSAQTECPHTLL*DAMFNSMTP
PPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFC
HQANLMSGPHSYGPARETNSCTEGPLFSSPRSA
VKLTKKRALSISPLSDASLDLQTVIRTSPSSLV
AFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNH
QKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPF
PTCQLKSELDMLVGKCREEPLEGDMSSPNSTGI
QDPLLGMLDGREDLEREEKREPESVYETDCRWDG
CSQEFDSQEQLVHHINSEHIHGERKEFVCHWGG
CSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEG
CRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKA
FSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYT
DPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAP
SISTVEPKREREGGPIREESRLTVPEGAMKPQPS
PGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGS
TEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLH
QLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLER
RSSSSSSISSAYTVSRRSSLASPFPPGSPPENG
ASSLPGLMPAQHYLLRARYASARGGGTSPTAAS
SLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASD
PAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQ
NFDPYLPTSVYSPQPPSITENAAMDARGLQEEP
EVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAE
PYGARGPGSLPLGPGPPTNYGPNPCPQQASYPD
PTQETWGEFPSHSGLYPGPKALGGTYSQCPRLE
HYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGP
PHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLP
SEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQE
LLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRA
KAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENF
VVGANRASHRAAAPPRLLPPLPTCYGPLKVGGT
NPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLD
LDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHT
PPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA*
RVGNLIHHRSHFLRGFYPSRKIGGAAVPCTRCPR
DGRYGLGAMYSLYTF*GEI***HCFLIIKELHQ
K

Frame 1

PDSSPGPRIPSPAPRQSVPTPSSETPCSTR*PH
HQSVAMASPAVSGPSPVRGPPVWGQKDCLARPS
ATKLTSCPAPTVMGQPERPTAAPRAHSFLLPGVQ
SS*PRSGHCPSHLCRMPAWTCRRLSAPHPAPS*
LSSTRDAHLQEAPTVISPLAP*AHLWDSQPR*I
TKKGPRLPLGSSLVVPMTLPGVG*SHILSPGDPS
QLAS*SLSWTCWLASAGRNPWKVICPAPTPQAY
RIPCWGCWMGGRTSRERRSVSLNLCMKLTAVGM
AAARNLTPKSSWCTTSTASTSTGSGRSSCATGGA
APGS*GPSKPSTCWWFTCADTLARSHTSARLKG
AGSHTHASKT*RRTCGHTRVRSHTCVSTRAAVK
PSAMPVTEPSTRIGPIPMRSRMYVSSLAAPNAIQ
ILARCENMSRQCMVLTPM*PNGTVGMAPCLGHH
PFLQWSPRGSGKEVPSGRKAD*LCQRVP*SHSQ
ALGPSHPAAVTTPRQGVQPIQTVVWK*LAMQGAA
LKTSPAWTRDLALLALVCPLFAALRTSGWTSYI
NSGQ*GPGVSNCPACPTPVPLCPAAWAPQSLLN
AAAAAPAASALPILSAAAPPWPLLSPLAPHQRME
HPPCLALCLPSTTCFGQDMLQPEGVVLRPLQHP
AWIG*VVFPCLLGEAEPSIQDTTPMQGSPGGPV
TQPRLLTVLLQLESRGSRAWAVSIPHPLWQGEDR
TLILTSQPLSTHHSPPASLRMLPWMLEGYRKSQ
KLGPPWWAVV*TPIWTSHLLILWDMGDLKGQQL
SLMERGVQALCLLGLVHPPTMAPTPVPSRPHILT
PPKKHGVSSLPTLGCTQAPRL*VEPTASVLDLN
IMDKCKSSQNRGAQWGLTPQDWHPASMPTPVRG
PHIHSLSFPITPSPLLPNISSQAPIPSHPLIIFL
QNPGLAWTLIPPPIPQGSSRLSLCVIMFNLNRS
YCGRVGAGKMPPPRNLPTRVPSFWGVPRLAQAV
LKLQ*THMDLALDPTCPITSQVPIPPLHHAMKIL
*WGQIGLHIGQQHHLDFCPHCPLAMGLSKWEAQ
TPAVVILRWAG*EGVLPCTLLPKDRYVTPWTLL
ILTTLSWTLWLFWMSPRG*VLLLPMISGAALDIP
HLPLGPPTWLWAT*VSY*DPYLGKQNSSTLVPK
E*GISSITDRIS*GVSILPEKLGELQSHAQDAP
GMGGMGWGLCIVCIRFEEKFDNDTVS***RNCIR
K

Frame 2

QTPALDRASRAQRPDRVSPHPPLRRHVQLDDPT
TNQ*LWRALLSPAPPQSGGPQCGDRRTVWPALL
PPS*PHVRPPQLWASQRDQQLHRGPTLFFSPEC
SQVDQEAGTVHLTSVGCQPGPADGYPHLTQLPRS
FHQLAMHISRRLLRSSLHWHHEPISGIPSPDES
PKRALAFLWGPALWSP*LCPGWDDPTSSVPGTL
PNLPAEV*AGHAGWQVPGGTLGR*YVQPQLHRHT
GSPVGDAGWAGGPRERGEA*A*ICV*N*LPLGW
LQPGI*LPRAAGAPHQQRAHPRGAEGVRVPLGG
LLQGAEALQSPVHAGGSHAQTHWREATQVHV*RV
PEVILTPRKPEDAPAVTHG*EAIHV*ARGLQ*S
LQQCQ*PSQAPESDPFQ*EAVCM*APWLHQTLY
RS*LAAKTCQDSAWS*RPCDQTAPWGWPPASGTI
HFYSGAQEGAGRRSHQGGKQTDCARGCHEATAK
PWGPVILQQ*PLPGRECSQYRQWCGNDWQCRGQ
H*RPLQLGRGTLHCWHWSVHSSPP*EPQAGPATS
TPANRDPGSQTAQLVPHRYHCVPPRGPPSLS*T
PQQQLQQHQLCLYCQPPLLPGLSFPPWLPTREW
SILPAWPYACPALPASGKICFSQRGWYFAHCSIQ
PGSDRWSSHASLEKPSRVSRIQPQCRGHPEGQ*
PSPGC*PSCSS*SPEVQEPGLCPYPTHCGRGRT
EL*SLPPNLCLLTTAPQHH*ECCHGC*RATGRAR
SWDLHGGQWSEPLYGLPTY*YSGIWGT*RGSS*
ALWSEGSRLSASWAWSTHQLWPQPLSPAGLIS*
PHPRNMG*VPFPLWAVPRPQGSRWNLQPVSST*T
LWTSASQARTGVPSGV*LHRTGTLPQCPPQ*GA
PTSTASLFPLPPALSSPISPVRPLYPATP*LSS
FRTQALPGL*FPHPFHRAAQGSACV*LCSISTGA
TVGGWGQGRCPRPGTFLPESQVSGGFPG*PKPC
*SSSEHIWTWLWTQLAQSQVRFLSHPFTMP*KF
CSGGK*GFT*GSSTTSTSAPIAHLLWASQSGRHK
PQLWSS*GGQARRGSCLVPSSRRTGM*PPGLS*
S*QHSAGLCGYSG*APGAESSSFP*SAGQLWTY
PTSLWAPQHGCGQHECLTEIPTWGNRIPQL*CLK
SRESHPSQIAFPKGFLSFQKNWGSCSPMHKMPQ
GWEVWAGGYV*SVYVLRRNLIMTLFPDNKGTAS
EK

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