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Sequences

Sequence PLD1.bAug05

ATGGTTAATAGTGAAAGATTCCTTTTTATTGTATATGAAACCAGACAGCGGTGCCATTGCCTTCGTCCTGCTGGTAGACAAAGAATTCAAAATTAAGGTG GGGAAGAAGGAGACAGAAACGAAATATGGAATCCGAATTGATAATCTTTCAAGGACACTTATTTTAAAATGCAACAGCTATAGACATGCTCGGTGGTGGG GAGGGGCTATAGAAGAATTCATCCAGAAACATGGCACCAACTTTCTCAAAGATCATCGATTTGGGTCATATGCTGCTATCCAAGAGAATGCTTTAGCTAA ATGGTATGTTAATGCCAAAGGATATTTTGAAGATGTGGCAAATGCAATGGAAGAGGCAAATGAAGAGATTTTTATCACAGACTGGTGGCTGAGTCCAGAA ATCTTCCTGAAACGCCCAGTGGTTGAGGGAAATCGTTGGAGGTTGGACTGCATTCTTAAACGAAAAGCACAACAAGGAGTGAGGATCTTCATAATGCTCT ACAAAGAGGTGGAACTCGCTCTTGGCATCAATAGTGAATACACCAAGAGGACTTTGATGCGTCTACATCCCAACATAAAGGTGATGAGACACCCGGATCA TGTGTCATCCACCGTCTATTTGTGGGCTCACCATGAGAAGCTTGTCATCATTGACCAATCGGTGGCCTTTGTGGGAGGGATTGACCTGGCCTATGGAAGG TGGGACGACAATGAGCACAGACTCACAGACGTGGGCAGTGTGAAGCGGGTCACTTCAGGACCGTCTCTGGGTTCCCTCCCACCTGCCGCAATGGAGTCTA TGGAATCCTTAAGACTCAAAGATAAAAATGAGCCTGTTCAAAACCTACCCATCCAGAAGAGTATTGATGATGTGGATTCAAAACTGAAAGGAATAGGAAA GCCAAGAAAGTTCTCCAAATTTAGTCTCTACAAGCAGCTCCACAGGCACCACCTGCACGACGCAGATAGCATCAGCAGCATTGACAGCACCTCCAATACC GGGTCCATCCGTAGTTTACAGACAGGTGTGGGAGAGCTGCATGGGGAAACCAGATTCTGGCATGGAAAGGACTACTGCAATTTCGTCTTCAAAGACTGGG TTCAACTTGATAAACCTTTTGCTGATTTCATTGACAGGTACTCCACGCCCCGGATGCCCTGGCATGACATTGCCTCTGCAGTCCACGGGAAGGCGGCTCG TGATGTGGCACGTCACTTCATCCAGCGCTGGAACTTCACAAAAATTATGAAATCAAAATATCGGTCCCTTTCTTATCCTTTTCTGCTTCCAAAGTCTCAA ACAACAGCCCATGAGTTGAGATATCAAGTGCCTGGGTCTGTCCATGCTAACGTACAGTTGCTCCGCTCTGCTGCTGATTGGTCTGCTGGTATAAAGTACC ATGAAGAGTCCATCCACGCCGCTTACGTCCATGTGATAGAGAACAGCAGGCACTATATCTATATCGAAAACCAGTTTTTCATAAGCTGTGCTGATGACAA AGTTGTGTTCAACAAGATAGGCGATGCCATTGCCCAGAGGATCCTGAAAGCTCACAGGGAAAACCAGAAATACCGGGTATATGTCGTGATACCACTTCTG CCAGGGTTCGAAGGAGACATTTCAACCGGCGGAGGAAATGCTCTACAGGCAATCATGCACTTCAACTACAGAACCATGTGCAGAGGAGAAAATTCCATCC TTGGACAGTTAAAAGCAGAGCTTGGTAATCAGTGGATAAATTACATATCATTCTGTGGTCTTAGAACACATGCAGAGCTCGAAGGAAACCTAGTAACTGA GCTTATCTATGTCCACAGCAAGTTGTTAATTGCTGATGATAACACTGTTATTATTGGCTCTGCCAACATAAATGACCGCAGCATGCTGGGAAAGCGTGAC AGTGAAATGGCTGTCATTGTGCAAGATACAGAGACTGTTCCTTCAGTAATGGATGGAAAAGAGTACCAAGCTGGCCGGTTTGCCCGAGGACTTCGGCTAC AGTGCTTTAGGGTTGTCCTTGGCTATCTTGATGACCCAAGTGAGGACATTCAGGATCCAGTGAGTGACAAATTCTTCAAGGAGGTGTGGGTTTCAACAGC AGCTCGAAATGCTACAATTTATGACAAGGTTTTCCGGTGCCTTCCCAATGATGAAGTACACAATTTAATTCAGCTGAGAGACTTTATAAACAAGCCCGTA TTAGCTAAGGAAGATCCCATTCGAGCTGAGGAGGAACTGAAGAAGATCCGTGGATTTTTGGTGCAATTCCCCTTTTATTTCTTGTCTGAAGAAAGCCTAC TGCCTTCTGTTGGGACCAAAGAGGCCATAGTGCCCATGGAGGTTTGGACTTAAGAGATATTCATTGGCAGCTCAAAGACTTCCACCCTGGAGACCACACT GCACACAGTGACTTCCTGGGGATGTCATAGCCAAAGCCAGGCCTGACGCATTCTCGTATCCAACCCAAGGACCTTTTGGAATGACTGGGGAGGGCTGCAG TCACATTGATGTAAGGACTGTAAACATCAGCAAGACTTTATAATTCCTTCTGCCTAACTTGTAAAAAGGGGGCTGCATTCTTGTTGGTAGCATGTACTCT GTTGAGTAAAACACATATTCAAATTCCGTATACCAAAATCCATTTCCTTTGTAACAAGAATTTACCAGTAACTGTGATCTAGGTTGCCAAAAGTTGTCTG AATCTCCTTATTCTTCTCTGATCTTCATTTATGCAGCCAATGTCTAGCTGGACCTGCCCTCATCTTGCAGTTCATACAGGCACTGTTTGAGAGATTGTTT ATTATTAGATGTTGTAATGCTGCTTCAAGATTCTTCATGGTTACATGGGATGCCCCTGCTCATCTGGTCCTGAAGTAGTAACATTCACCCAAATGAGGAT ATAGTCATTATTCCTTTTCAGTCACTGTACGAACACGGCAGACTTTAGCCTACACAGTAGACTGTGTTAGGCACTTCTGGTAACATTGACACTGTATCTT GACACCACTAAGCAACAGGAAGGAATAAATTCCAATATTGAGACAGAGATTTATTTCATTTTGCTCCCAAAGGCACTGACAACATGAGTCCTTGTGATTA GGCCCACCTGATCAAATGTAAAAACATGCATGGGATATTTGATTACGTAACAACCAGCTAAAACTGAGAGCACACAGCCTTCGGAAACCCCCAAGGTGTC GAGGAGGATCAGATCCAGATAAGAGAAGATGATTCCTTCTAGTCCTGTAAAATTTCCTGTCATCCTTAGCTGCTGGGTTGATTGAAATTTGATCTCTACA TTTCAAAATCAAACTAGTGAATACAGTTTGTCTTTCAAGAGAATCAGAGGCATCGTAACTCCTACCAAGTTAGTAAGTATGAGTCTAACCTTGTTTGACA TTCAGGTCTTCTGCTACATTATCTTCTTTGAGAAAAATTAATTGATTATAAAATATGTGCTACTTCACTTACATTATTAAATATGTATTAACGCCTCTCA CCAGGGCTTCCAGTGAAGTTACAATGCCCTAGTCTGTGAATTAGTCTGGAAACGTGTTTTTCCTTTTCGGATGTTAGAGTACCCTTTGATAAACTAAATT TTACTAAGCTGAACAACTCTGACAGTCTAAAGAGCTAATGTGGGTTACCAAAAGGCCTGTACCTGTAAAACAAAATGCAGGTGTAATGATTATACATGTC TATGGATTACCTGGACATACTCTCATTTGGGTTGTTCTTCAAAGAAGCAAGCAGCCGATCCCTGTTTTCATAAAGCTAATACTTCAGTTGGAAAAATTAA ACAGGAGCACAAAGTCAGGGATAGGGGTTAGCAGAAGAGAGAAATAGTGTCACATCAAGGGCAGGATCTCATAGCTAGGGAACATTTCACAAATAAGGTG AGATTTTGTAACCAATAATAAAAATGAATGTTTTTATAAGTAAATAACTTATTTTTCATATGGCTAAAGATGGTAAAATGACTTCATTCTATAGCCATTG TAAATAAGAATTTGCTATTGATGAAAGAAGTTCAGATTGGCATTTGAAGTATTGAGTGTATGGGATCTCTAAGGATTTCTTAGATTTTATATTTAAATAT TTTTTAAACCTTAGAGGAGTCAACAAACTGGCTCTTGATTTTCAGCACCCTACTCTCATGAAAAAAGCCTGAAAGGACCCTTTCCCTTATAAGTAATTTA ATCCAATTTCTCCCCATTTTATAGATGAGGAAACTGAGGCTCAGATCAGATGAGAACTCACTTAAATCCACTCAATGTGTAGATGGTAGAGCTGGGACTA GCAACATTGCTGCAGCCCATTGTTGGCCTCTCTCTTCACTTTATCATTGCCCAAGAATGAGGATATGCAGTAAACAGAATTCAGGCAAGATACCTCTAAG CTGTTTTGAACCCTCTGATATTTTGTATTTATGTGTTTGTCTGTCTCCCCCTACTAGAATGTAAGCTCCATGGGGCAGGGACTTCACTGTATTTTGTTCA TAGTGTATCCCCAGAGCCTGGACCAGTGCTTGGCACATAGGAGATGGCAATAAATGCTTGTAGAATTAATAAACAAGGTGAAGGAGAGAGCTAATGAAAT GAAAATCTTAGTCTCATATAAGAAGTCTTACTACTGCTTTCTATGGCAAGTTCCTTTTCTATTGCATCTTCCCAAAGACAAGATGCTCATCTGCAAAGGG GTGATAAGATTTACTTGGAGGGATTTTATGGAAAATTCAGTGCAATAATTGCTTTTTTTTTTTTTTTTGCAGTAGCAGTTTACATAGTACAGCATTCTGC AAAAATTGCATGAAATGTGGACACAAGATGTACTGTATGACTTAATTACCTGTCATTCTCTGACACCATTACTACCTACTAGAAGTTCAAATAATTTATC TTTAACATTGCAATATCTGATAAACTTTTTAAAATGTAAATAATTCAAAGCCTTGAAAATCTATCATGAATCTGCTCAACATTTCAAAGTGACTATTTTG AAATGCCTATAAATGCTATATACCTG

PLD1.F2 (forward primer)
PLD1.R2 (reverse primer)
Single exon skipping

Frame 0

MVNSERFLFIVYETRQRCHCLRPAGRQRIQN*G GEEGDRNEIWNPN**SFKDTYFKMQQL*TCSVVG RGYRRIHPETWHQLSQRSSIWVICCYPRECFS* MVC*CQRIF*RCGKCNGRGK*RDFYHRLVAESR NLPETPSG*GKSLEVGLHS*TKSTTRSEDLHNAL QRGGTRSWHQ**IHQEDFDASTSQHKGDETPGS CVIHRLFVGSP*EACHH*PIGGLCGRD*PGLWK VGRQ*AQTHRRGQCEAGHFRTVSGFPPTCRNGVY GILKTQR*K*ACSKPTHPEEY**CGFKTERNRK AKKVLQI*SLQAAPQAPPARRR*HQQH*QHLQY RVHP*FTDRCGRAAWGNQILAWKGLLQFRLQRLG ST**TFC*FH*QVLHAPDALA*HCLCSPREGGS *CGTSLHPALELHKNYEIKISVPFLSFSASKVS NNSP*VEISSAWVCPC*RTVAPLCC*LVCWYKVP *RVHPRRLRPCDREQQALYLYRKPVFHKLC**Q SCVQQDRRCHCPEDPESSQGKPEIPGICRDTTS ARVRRRHFNRRRKCSTGNHALQLQNHVQRRKFHP WTVKSRAW*SVDKLHIILWS*NTCRARRKPSN* AYLCPQQVVNC***HCYYWLCQHK*PQHAGKA* Q*NGCHCARYRDCSFSNGWKRVPSWPVCPRTSAT VL*GCPWLS**PK*GHSGSSE*QILQGGVGFNS SSKCYNL*QGFPVPSQ**STQFNSAERLYKQAR IS*GRSHSS*GGTEEDPWIFGAIPLLFLV*RKPT AFCWDQRGHSAHGGLDLRDIHWQLKDFHPGDHT AHSDFLGMS*PKPGLTHSRIQPKDLLE*LGRAA VTLM*GL*TSARLYNSFCLTCKKGAAFLLVACTL LSKTHIQIPYTKIHFLCNKNLPVTVI*VAKSCL NLLILL*SSFMQPMSSWTCPHLAVHTGTV*EIV YY*ML*CCFKILHGYMGCPCSSGPEVVTFTQMRI *SLFLFSHCTNTADFSLHSRLC*ALLVTLTLYL DTTKQQEGINSNIETEIYFILLPKALTT*VLVI RPT*SNVKTCMGYLIT*QPAKTESTQPSETPKVS RRIRSR*EKMIPSSPVKFPVILSCWVD*NLIST FQNQTSEYSLSFKRIRGIVTPTKLVSMSLTLFD IQVFCYIIFFEKN*LIIKYVLLHLHY*ICINASH QGFQ*SYNALVCELVWKRVFPFRMLEYPLIN*I LLS*TTLTV*RANVGYQKACTCKTKCRCNDYTC LWITWTYSHLGCSSKKQAADPCFHKANTSVGKIK QEHKVRDRG*QKREIVSHQGQDLIAREHFTNKV RFCNQ**K*MFL*VNNLFFIWLKMVK*LHSIAI VNKNLLLMKEVQIGI*SIECMGSLRIS*ILYLNI F*TLEESTNWLLIFSTLLS*KKPERTLSLISNL IQFLPIL*MRKLRLRSDENSLKSTQCVDGRAGT SNIAAAHCWPLSSLYHCPRMRICSKQNSGKIPLS CFEPSDILYLCVCLSPPTRM*APWGRDFTVFCS *CIPRAWTSAWHIGDGNKCL*N**TR*RRELMK *KS*SHIRSLTTAFYGKFLFYCIFPKTRCSSAKG **DLLGGILWKIQCNNCFFFFFCSSSLHSTAFC KNCMKCGHKMYCMT*LPVIL*HHYYLLEVQIIY L*HCNI**TF*NVNNSKP*KSIMNLLNISK*LF* NAYKCYIP

Frame 1

WLIVKDSFLLYMKPDSGAIAFVLLVDKEFKIKV GKKETETKYGIRIDNLSRTLILKCNSYRHARWW GGAIEEFIQKHGTNFLKDHRFGSYAAIQENALAK WYVNAKGYFEDVANAMEEANEEIFITDWWLSPE IFLKRPVVEGNRWRLDCILKRKAQQGVRIFIML YKEVELALGINSEYTKRTLMRLHPNIKVMRHPDH VSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGR WDDNEHRLTDVGSVKRVTSGPSLGSLPPAAMES MESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGK PRKFSKFSLYKQLHRHHLHDADSISSIDSTSNT GSIRSLQTGVGELHGETRFWHGKDYCNFVFKDW VQLDKPFADFIDRYSTPRMPWHDIASAVHGKAAR DVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQ TTAHELRYQVPGSVHANVQLLRSAADWSAGIKY HEESIHAAYVHVIENSRHYIYIENQFFISCADDK VVFNKIGDAIAQRILKAHRENQKYRVYVVIPLL PGFEGDISTGGGNALQAIMHFNYRTMCRGENSI LGQLKAELGNQWINYISFCGLRTHAELEGNLVTE LIYVHSKLLIADDNTVIIGSANINDRSMLGKRD SEMAVIVQDTETVPSVMDGKEYQAGRFARGLRL QCFRVVLGYLDDPSEDIQDPVSDKFFKEVWVSTA ARNATIYDKVFRCLPNDEVHNLIQLRDFINKPV LAKEDPIRAEEELKKIRGFLVQFPFYFLSEESL LPSVGTKEAIVPMEVWT*EIFIGSSKTSTLETTL HTVTSWGCHSQSQA*RILVSNPRTFWNDWGGLQ SH*CKDCKHQQDFIIPSA*LVKRGLHSCW*HVL C*VKHIFKFRIPKSISFVTRIYQ*L*SRLPKVV* ISLFFSDLHLCSQCLAGPALILQFIQALFERLF IIRCCNAASRFFMVTWDAPAHLVLK**HSPK*G YSHYSFSVTVRTRQTLAYTVDCVRHFW*H*HCIL TPLSNRKE*IPILRQRFISFCSQRH*QHESL*L GPPDQM*KHAWDI*LRNNQLKLRAHSLRKPPRC RGGSDPDKRR*FLLVL*NFLSSLAAGLIEI*SLH FKIKLVNTVCLSRESEAS*LLPS**V*V*PCLT FRSSATLSSLRKIN*L*NMCYFTYIIKYVLTPL TRASSEVTMP*SVN*SGNVFFLFGC*STL**TKF Y*AEQL*QSKELMWVTKRPVPVKQNAGVMIIHV YGLPGHTLIWVVLQRSKQPIPVFIKLILQLEKL NRSTKSGIGVSRREK*CHIKGRIS*LGNISQIR* DFVTNNKNECFYK*ITYFSYG*RW*NDFIL*PL *IRICY**KKFRLAFEVLSVWDL*GFLRFYI*I FFKP*RSQQTGS*FSAPYSHEKSLKGPFPL*VI* SNFSPFYR*GN*GSDQMRTHLNPLNV*MVELGL ATLLQPIVGLSLHFIIAQE*GYAVNRIQARYL* AVLNPLIFCIYVFVCLPLLECKLHGAGTSLYFVH SVSPEPGPVLGT*EMAINACRINKQGEGES**N ENLSLI*EVLLLLSMASSFSIASSQRQDAHLQR GDKIYLEGFYGKFSAIIAFFFFFAVAVYIVQHSA KIA*NVDTRCTV*LNYLSFSDTITTY*KFK*FI FNIAISDKLFKM*IIQSLENLS*ICSTFQSDYF EMPINAIYL

Frame 2

G***KIPFYCI*NQTAVPLPSSCW*TKNSKLRW GRRRQKRNMESELIIFQGHLF*NATAIDMLGGG EGL*KNSSRNMAPTFSKIIDLGHMLLSKRML*L NGMLMPKDILKMWQMQWKRQMKRFLSQTGG*VQK SS*NAQWLREIVGGWTAFLNEKHNKE*GSS*CS TKRWNSLLASIVNTPRGL*CVYIPT*R**DTRI MCHPPSICGLTMRSLSSLTNRWPLWEGLTWPMEG GTTMSTDSQTWAV*SGSLQDRLWVPSHLPQWSL WNP*DSKIKMSLFKTYPSRRVLMMWIQN*KE*E SQESSPNLVSTSSSTGTTCTTQIASAALTAPPIP GPSVVYRQVWESCMGKPDSGMERTTAISSSKTG FNLINLLLISLTGTPRPGCPGMTLPLQSTGRRL VMWHVTSSSAGTSQKL*NQNIGPFLILFCFQSLK QQPMS*DIKCLGLSMLTYSCSALLLIGLLV*ST MKSPSTPLTSM**RTAGTISISKTSFS*AVLMT KLCSTR*AMPLPRGS*KLTGKTRNTGYMS*YHFC QGSKETFQPAEEMLYRQSCTSTTEPCAEEKIPS LDS*KQSLVISG*ITYHSVVLEHMQSSKET**L SLSMSTASC*LLMITLLLLALPT*MTAACWESVT VKWLSLCKIQRLFLQ*WMEKSTKLAGLPEDFGY SALGLSLAILMTQVRTFRIQ*VTNSSRRCGFQQ QLEMLQFMTRFSGAFPMMKYTI*FS*ETL*TSPY *LRKIPFELRRN*RRSVDFWCNSPFISCLKKAY CLLLGPKRP*CPWRFGLKRYSLAAQRLPPWRPH CTQ*LPGDVIAKARPDAFSYPTQGPFGMTGEGCS HIDVRTVNISKTL*FLLPNL*KGGCILVGSMYS VE*NTYSNSVYQNPFPL*QEFTSNCDLGCQKLS ESPYSSLIFIYAANV*LDLPSSCSSYRHCLRDCL LLDVVMLLQDSSWLHGMPLLIWS*SSNIHPNED IVIIPFQSLYEHGRL*PTQ*TVLGTSGNIDTVS *HH*ATGRNKFQY*DRDLFHFAPKGTDNMSPCD* AHLIKCKNMHGIFDYVTTS*N*EHTAFGNPQGV EEDQIQIREDDSF*SCKISCHP*LLG*LKFDLY ISKSN**IQFVFQENQRHRNSYQVSKYESNLV*H SGLLLHYLL*EKLIDYKICATSLTLLNMY*RLS PGLPVKLQCPSL*ISLETCFSFSDVRVPFDKLN FTKLNNSDSLKS*CGLPKGLYL*NKMQV**LYMS MDYLDILSFGLFFKEASSRSLFS*S*YFSWKN* TGAQSQG*GLAEERNSVTSRAGSHS*GTFHK*G EIL*PIIKMNVFISK*LIFHMAKDGKMTSFYSHC K*EFAIDERSSDWHLKY*VYGISKDFLDFIFKY FLNLRGVNKLALDFQHPTLMKKA*KDPFPYK*F NPISPHFIDEETEAQIR*ELT*IHSMCRW*SWD* QHCCSPLLASLFTLSLPKNEDMQ*TEFRQDTSK LF*TL*YFVFMCLSVSPY*NVSSMGQGLHCILF IVYPQSLDQCLAHRRWQ*MLVELINKVKERANEM KILVSYKKSYYCFLWQVPFLLHLPKDKMLICKG VIRFTWRDFMENSVQ*LLFFFFLQ*QFT*YSIL QKLHEMWTQDVLYDLITCHSLTPLLPTRSSNNLS LTLQYLINFLKCK*FKALKIYHESAQHFKVTIL KCL*MLYT

Sequence PLD1.aAug05

ATGGTTAATAGTGAAAGATTCCTTTTTATTGTATATGAAACCAGACAGCGGTGCCATTGCCTTCGTCCTGCTGGTAGACAAAGAATTCAAAATTAAGGTG GGGAAGAAGGAGACAGAAACGAAATATGGAATCCGAATTGATAATCTTTCAAGGACACTTATTTTAAAATGCAACAGCTATAGACATGCTCGGTGGTGGG GAGGGGCTATAGAAGAATTCATCCAGAAACATGGCACCAACTTTCTCAAAGATCATCGATTTGGGTCATATGCTGCTATCCAAGAGAATGCTTTAGCTAA ATGGTATGTTAATGCCAAAGGATATTTTGAAGATGTGGCAAATGCAATGGAAGAGGCAAATGAAGAGATTTTTATCACAGACTGGTGGCTGAGTCCAGAA ATCTTCCTGAAACGCCCAGTGGTTGAGGGAAATCGTTGGAGGTTGGACTGCATTCTTAAACGAAAAGCACAACAAGGAGTGAGGATCTTCATAATGCTCT ACAAAGAGGTGGAACTCGCTCTTGGCATCAATAGTGAATACACCAAGAGGACTTTGATGCGTCTACATCCCAACATAAAGGTGATGAGACACCCGGATCA TGTGTCATCCACCGTCTATTTGTGGGCTCACCATGAGAAGCTTGTCATCATTGACCAATCGGTGGCCTTTGTGGGAGGGATTGACCTGGCCTATGGAAGG TGGGACGACAATGAGCACAGACTCACAGACGTGGGCAGTGTGAAGCGGGTCACTTCAGGACCGTCTCTGGGTTCCCTCCCACCTGCCGCAATGGAGTCTA TGGAATCCTTAAGACTCAAAGATAAAAATGAGCCTGTTCAAAACCTACCCATCCAGAAGAGTATTGATGATGTGGATTCAAAACTGAAAGGAATAGGAAA GCCAAGAAAGTTCTCCAAATTTAGTCTCTACAAGCAGCTCCACAGGCACCACCTGCACGACGCAGATAGCATCAGCAGCATTGACAGCACCTCCAGTTAT TTTAATCACTATAGAAGTCATCACAATTTAATCCATGGTTTAAAACCCCACTTCAAACTCTTTCACCCGTCCAGTGAGTCTGAGCAAGGACTCACTAGAC CTCATGCTGATACCGGGTCCATCCGTAGTTTACAGACAGGTGTGGGAGAGCTGCATGGGGAAACCAGATTCTGGCATGGAAAGGACTACTGCAATTTCGT CTTCAAAGACTGGGTTCAACTTGATAAACCTTTTGCTGATTTCATTGACAGGTACTCCACGCCCCGGATGCCCTGGCATGACATTGCCTCTGCAGTCCAC GGGAAGGCGGCTCGTGATGTGGCACGTCACTTCATCCAGCGCTGGAACTTCACAAAAATTATGAAATCAAAATATCGGTCCCTTTCTTATCCTTTTCTGC TTCCAAAGTCTCAAACAACAGCCCATGAGTTGAGATATCAAGTGCCTGGGTCTGTCCATGCTAACGTACAGTTGCTCCGCTCTGCTGCTGATTGGTCTGC TGGTATAAAGTACCATGAAGAGTCCATCCACGCCGCTTACGTCCATGTGATAGAGAACAGCAGGCACTATATCTATATCGAAAACCAGTTTTTCATAAGC TGTGCTGATGACAAAGTTGTGTTCAACAAGATAGGCGATGCCATTGCCCAGAGGATCCTGAAAGCTCACAGGGAAAACCAGAAATACCGGGTATATGTCG TGATACCACTTCTGCCAGGGTTCGAAGGAGACATTTCAACCGGCGGAGGAAATGCTCTACAGGCAATCATGCACTTCAACTACAGAACCATGTGCAGAGG AGAAAATTCCATCCTTGGACAGTTAAAAGCAGAGCTTGGTAATCAGTGGATAAATTACATATCATTCTGTGGTCTTAGAACACATGCAGAGCTCGAAGGA AACCTAGTAACTGAGCTTATCTATGTCCACAGCAAGTTGTTAATTGCTGATGATAACACTGTTATTATTGGCTCTGCCAACATAAATGACCGCAGCATGC TGGGAAAGCGTGACAGTGAAATGGCTGTCATTGTGCAAGATACAGAGACTGTTCCTTCAGTAATGGATGGAAAAGAGTACCAAGCTGGCCGGTTTGCCCG AGGACTTCGGCTACAGTGCTTTAGGGTTGTCCTTGGCTATCTTGATGACCCAAGTGAGGACATTCAGGATCCAGTGAGTGACAAATTCTTCAAGGAGGTG TGGGTTTCAACAGCAGCTCGAAATGCTACAATTTATGACAAGGTTTTCCGGTGCCTTCCCAATGATGAAGTACACAATTTAATTCAGCTGAGAGACTTTA TAAACAAGCCCGTATTAGCTAAGGAAGATCCCATTCGAGCTGAGGAGGAACTGAAGAAGATCCGTGGATTTTTGGTGCAATTCCCCTTTTATTTCTTGTC TGAAGAAAGCCTACTGCCTTCTGTTGGGACCAAAGAGGCCATAGTGCCCATGGAGGTTTGGACTTAAGAGATATTCATTGGCAGCTCAAAGACTTCCACC CTGGAGACCACACTGCACACAGTGACTTCCTGGGGATGTCATAGCCAAAGCCAGGCCTGACGCATTCTCGTATCCAACCCAAGGACCTTTTGGAATGACT GGGGAGGGCTGCAGTCACATTGATGTAAGGACTGTAAACATCAGCAAGACTTTATAATTCCTTCTGCCTAACTTGTAAAAAGGGGGCTGCATTCTTGTTG GTAGCATGTACTCTGTTGAGTAAAACACATATTCAAATTCCGTATACCAAAATCCATTTCCTTTGTAACAAGAATTTACCAGTAACTGTGATCTAGGTTG CCAAAAGTTGTCTGAATCTCCTTATTCTTCTCTGATCTTCATTTATGCAGCCAATGTCTAGCTGGACCTGCCCTCATCTTGCAGTTCATACAGGCACTGT TTGAGAGATTGTTTATTATTAGATGTTGTAATGCTGCTTCAAGATTCTTCATGGTTACATGGGATGCCCCTGCTCATCTGGTCCTGAAGTAGTAACATTC ACCCAAATGAGGATATAGTCATTATTCCTTTTCAGTCACTGTACGAACACGGCAGACTTTAGCCTACACAGTAGACTGTGTTAGGCACTTCTGGTAACAT TGACACTGTATCTTGACACCACTAAGCAACAGGAAGGAATAAATTCCAATATTGAGACAGAGATTTATTTCATTTTGCTCCCAAAGGCACTGACAACATG AGTCCTTGTGATTAGGCCCACCTGATCAAATGTAAAAACATGCATGGGATATTTGATTACGTAACAACCAGCTAAAACTGAGAGCACACAGCCTTCGGAA ACCCCCAAGGTGTCGAGGAGGATCAGATCCAGATAAGAGAAGATGATTCCTTCTAGTCCTGTAAAATTTCCTGTCATCCTTAGCTGCTGGGTTGATTGAA ATTTGATCTCTACATTTCAAAATCAAACTAGTGAATACAGTTTGTCTTTCAAGAGAATCAGAGGCATCGTAACTCCTACCAAGTTAGTAAGTATGAGTCT AACCTTGTTTGACATTCAGGTCTTCTGCTACATTATCTTCTTTGAGAAAAATTAATTGATTATAAAATATGTGCTACTTCACTTACATTATTAAATATGT ATTAACGCCTCTCACCAGGGCTTCCAGTGAAGTTACAATGCCCTAGTCTGTGAATTAGTCTGGAAACGTGTTTTTCCTTTTCGGATGTTAGAGTACCCTT TGATAAACTAAATTTTACTAAGCTGAACAACTCTGACAGTCTAAAGAGCTAATGTGGGTTACCAAAAGGCCTGTACCTGTAAAACAAAATGCAGGTGTAA TGATTATACATGTCTATGGATTACCTGGACATACTCTCATTTGGGTTGTTCTTCAAAGAAGCAAGCAGCCGATCCCTGTTTTCATAAAGCTAATACTTCA GTTGGAAAAATTAAACAGGAGCACAAAGTCAGGGATAGGGGTTAGCAGAAGAGAGAAATAGTGTCACATCAAGGGCAGGATCTCATAGCTAGGGAACATT TCACAAATAAGGTGAGATTTTGTAACCAATAATAAAAATGAATGTTTTTATAAGTAAATAACTTATTTTTCATATGGCTAAAGATGGTAAAATGACTTCA TTCTATAGCCATTGTAAATAAGAATTTGCTATTGATGAAAGAAGTTCAGATTGGCATTTGAAGTATTGAGTGTATGGGATCTCTAAGGATTTCTTAGATT TTATATTTAAATATTTTTTAAACCTTAGAGGAGTCAACAAACTGGCTCTTGATTTTCAGCACCCTACTCTCATGAAAAAAGCCTGAAAGGACCCTTTCCC TTATAAGTAATTTAATCCAATTTCTCCCCATTTTATAGATGAGGAAACTGAGGCTCAGATCAGATGAGAACTCACTTAAATCCACTCAATGTGTAGATGG TAGAGCTGGGACTAGCAACATTGCTGCAGCCCATTGTTGGCCTCTCTCTTCACTTTATCATTGCCCAAGAATGAGGATATGCAGTAAACAGAATTCAGGC AAGATACCTCTAAGCTGTTTTGAACCCTCTGATATTTTGTATTTATGTGTTTGTCTGTCTCCCCCTACTAGAATGTAAGCTCCATGGGGCAGGGACTTCA CTGTATTTTGTTCATAGTGTATCCCCAGAGCCTGGACCAGTGCTTGGCACATAGGAGATGGCAATAAATGCTTGTAGAATTAATAAACAAGGTGAAGGAG AGAGCTAATGAAATGAAAATCTTAGTCTCATATAAGAAGTCTTACTACTGCTTTCTATGGCAAGTTCCTTTTCTATTGCATCTTCCCAAAGACAAGATGC TCATCTGCAAAGGGGTGATAAGATTTACTTGGAGGGATTTTATGGAAAATTCAGTGCAATAATTGCTTTTTTTTTTTTTTTTGCAGTAGCAGTTTACATA GTACAGCATTCTGCAAAAATTGCATGAAATGTGGACACAAGATGTACTGTATGACTTAATTACCTGTCATTCTCTGACACCATTACTACCTACTAGAAGT TCAAATAATTTATCTTTAACATTGCAATATCTGATAAACTTTTTAAAATGTAAATAATTCAAAGCCTTGAAAATCTATCATGAATCTGCTCAACATTTCA AAGTGACTATTTTGAAATGCCTATAAATGCTATATACCTG

PLD1.F2 (forward primer)
PLD1.R2 (reverse primer)
Single exon skipping

Frame 0

MVNSERFLFIVYETRQRCHCLRPAGRQRIQN*G GEEGDRNEIWNPN**SFKDTYFKMQQL*TCSVVG RGYRRIHPETWHQLSQRSSIWVICCYPRECFS* MVC*CQRIF*RCGKCNGRGK*RDFYHRLVAESR NLPETPSG*GKSLEVGLHS*TKSTTRSEDLHNAL QRGGTRSWHQ**IHQEDFDASTSQHKGDETPGS CVIHRLFVGSP*EACHH*PIGGLCGRD*PGLWK VGRQ*AQTHRRGQCEAGHFRTVSGFPPTCRNGVY GILKTQR*K*ACSKPTHPEEY**CGFKTERNRK AKKVLQI*SLQAAPQAPPARRR*HQQH*QHLQL F*SL*KSSQFNPWFKTPLQTLSPVQ*V*ARTH*T SC*YRVHP*FTDRCGRAAWGNQILAWKGLLQFR LQRLGST**TFC*FH*QVLHAPDALA*HCLCSP REGGS*CGTSLHPALELHKNYEIKISVPFLSFSA SKVSNNSP*VEISSAWVCPC*RTVAPLCC*LVC WYKVP*RVHPRRLRPCDREQQALYLYRKPVFHK LC**QSCVQQDRRCHCPEDPESSQGKPEIPGICR DTTSARVRRRHFNRRRKCSTGNHALQLQNHVQR RKFHPWTVKSRAW*SVDKLHIILWS*NTCRARR KPSN*AYLCPQQVVNC***HCYYWLCQHK*PQHA GKA*Q*NGCHCARYRDCSFSNGWKRVPSWPVCP RTSATVL*GCPWLS**PK*GHSGSSE*QILQGG VGFNSSSKCYNL*QGFPVPSQ**STQFNSAERLY KQARIS*GRSHSS*GGTEEDPWIFGAIPLLFLV *RKPTAFCWDQRGHSAHGGLDLRDIHWQLKDFH PGDHTAHSDFLGMS*PKPGLTHSRIQPKDLLE*L GRAAVTLM*GL*TSARLYNSFCLTCKKGAAFLL VACTLLSKTHIQIPYTKIHFLCNKNLPVTVI*V AKSCLNLLILL*SSFMQPMSSWTCPHLAVHTGTV *EIVYY*ML*CCFKILHGYMGCPCSSGPEVVTF TQMRI*SLFLFSHCTNTADFSLHSRLC*ALLVT LTLYLDTTKQQEGINSNIETEIYFILLPKALTT* VLVIRPT*SNVKTCMGYLIT*QPAKTESTQPSE TPKVSRRIRSR*EKMIPSSPVKFPVILSCWVD* NLISTFQNQTSEYSLSFKRIRGIVTPTKLVSMSL TLFDIQVFCYIIFFEKN*LIIKYVLLHLHY*IC INASHQGFQ*SYNALVCELVWKRVFPFRMLEYP LIN*ILLS*TTLTV*RANVGYQKACTCKTKCRCN DYTCLWITWTYSHLGCSSKKQAADPCFHKANTS VGKIKQEHKVRDRG*QKREIVSHQGQDLIAREH FTNKVRFCNQ**K*MFL*VNNLFFIWLKMVK*LH SIAIVNKNLLLMKEVQIGI*SIECMGSLRIS*I LYLNIF*TLEESTNWLLIFSTLLS*KKPERTLS LISNLIQFLPIL*MRKLRLRSDENSLKSTQCVDG RAGTSNIAAAHCWPLSSLYHCPRMRICSKQNSG KIPLSCFEPSDILYLCVCLSPPTRM*APWGRDF TVFCS*CIPRAWTSAWHIGDGNKCL*N**TR*RR ELMK*KS*SHIRSLTTAFYGKFLFYCIFPKTRC SSAKG**DLLGGILWKIQCNNCFFFFFCSSSLH STAFCKNCMKCGHKMYCMT*LPVIL*HHYYLLEV QIIYL*HCNI**TF*NVNNSKP*KSIMNLLNIS K*LF*NAYKCYIP

Frame 1

WLIVKDSFLLYMKPDSGAIAFVLLVDKEFKIKV GKKETETKYGIRIDNLSRTLILKCNSYRHARWW GGAIEEFIQKHGTNFLKDHRFGSYAAIQENALAK WYVNAKGYFEDVANAMEEANEEIFITDWWLSPE IFLKRPVVEGNRWRLDCILKRKAQQGVRIFIML YKEVELALGINSEYTKRTLMRLHPNIKVMRHPDH VSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGR WDDNEHRLTDVGSVKRVTSGPSLGSLPPAAMES MESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGK PRKFSKFSLYKQLHRHHLHDADSISSIDSTSSY FNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTR PHADTGSIRSLQTGVGELHGETRFWHGKDYCNFV FKDWVQLDKPFADFIDRYSTPRMPWHDIASAVH GKAARDVARHFIQRWNFTKIMKSKYRSLSYPFL LPKSQTTAHELRYQVPGSVHANVQLLRSAADWSA GIKYHEESIHAAYVHVIENSRHYIYIENQFFIS CADDKVVFNKIGDAIAQRILKAHRENQKYRVYV VIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRG ENSILGQLKAELGNQWINYISFCGLRTHAELEG NLVTELIYVHSKLLIADDNTVIIGSANINDRSM LGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFAR GLRLQCFRVVLGYLDDPSEDIQDPVSDKFFKEV WVSTAARNATIYDKVFRCLPNDEVHNLIQLRDF INKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLS EESLLPSVGTKEAIVPMEVWT*EIFIGSSKTST LETTLHTVTSWGCHSQSQA*RILVSNPRTFWND WGGLQSH*CKDCKHQQDFIIPSA*LVKRGLHSCW *HVLC*VKHIFKFRIPKSISFVTRIYQ*L*SRL PKVV*ISLFFSDLHLCSQCLAGPALILQFIQAL FERLFIIRCCNAASRFFMVTWDAPAHLVLK**HS PK*GYSHYSFSVTVRTRQTLAYTVDCVRHFW*H *HCILTPLSNRKE*IPILRQRFISFCSQRH*QH ESL*LGPPDQM*KHAWDI*LRNNQLKLRAHSLRK PPRCRGGSDPDKRR*FLLVL*NFLSSLAAGLIE I*SLHFKIKLVNTVCLSRESEAS*LLPS**V*V *PCLTFRSSATLSSLRKIN*L*NMCYFTYIIKYV LTPLTRASSEVTMP*SVN*SGNVFFLFGC*STL **TKFY*AEQL*QSKELMWVTKRPVPVKQNAGV MIIHVYGLPGHTLIWVVLQRSKQPIPVFIKLILQ LEKLNRSTKSGIGVSRREK*CHIKGRIS*LGNI SQIR*DFVTNNKNECFYK*ITYFSYG*RW*NDF IL*PL*IRICY**KKFRLAFEVLSVWDL*GFLRF YI*IFFKP*RSQQTGS*FSAPYSHEKSLKGPFP L*VI*SNFSPFYR*GN*GSDQMRTHLNPLNV*M VELGLATLLQPIVGLSLHFIIAQE*GYAVNRIQA RYL*AVLNPLIFCIYVFVCLPLLECKLHGAGTS LYFVHSVSPEPGPVLGT*EMAINACRINKQGEG ES**NENLSLI*EVLLLLSMASSFSIASSQRQDA HLQRGDKIYLEGFYGKFSAIIAFFFFFAVAVYI VQHSAKIA*NVDTRCTV*LNYLSFSDTITTY*K FK*FIFNIAISDKLFKM*IIQSLENLS*ICSTFQ SDYFEMPINAIYL

Frame 2

G***KIPFYCI*NQTAVPLPSSCW*TKNSKLRW GRRRQKRNMESELIIFQGHLF*NATAIDMLGGG EGL*KNSSRNMAPTFSKIIDLGHMLLSKRML*L NGMLMPKDILKMWQMQWKRQMKRFLSQTGG*VQK SS*NAQWLREIVGGWTAFLNEKHNKE*GSS*CS TKRWNSLLASIVNTPRGL*CVYIPT*R**DTRI MCHPPSICGLTMRSLSSLTNRWPLWEGLTWPMEG GTTMSTDSQTWAV*SGSLQDRLWVPSHLPQWSL WNP*DSKIKMSLFKTYPSRRVLMMWIQN*KE*E SQESSPNLVSTSSSTGTTCTTQIASAALTAPPVI LITIEVITI*SMV*NPTSNSFTRPVSLSKDSLD LMLIPGPSVVYRQVWESCMGKPDSGMERTTAIS SSKTGFNLINLLLISLTGTPRPGCPGMTLPLQST GRRLVMWHVTSSSAGTSQKL*NQNIGPFLILFC FQSLKQQPMS*DIKCLGLSMLTYSCSALLLIGL LV*STMKSPSTPLTSM**RTAGTISISKTSFS*A VLMTKLCSTR*AMPLPRGS*KLTGKTRNTGYMS *YHFCQGSKETFQPAEEMLYRQSCTSTTEPCAE EKIPSLDS*KQSLVISG*ITYHSVVLEHMQSSKE T**LSLSMSTASC*LLMITLLLLALPT*MTAAC WESVTVKWLSLCKIQRLFLQ*WMEKSTKLAGLP EDFGYSALGLSLAILMTQVRTFRIQ*VTNSSRRC GFQQQLEMLQFMTRFSGAFPMMKYTI*FS*ETL *TSPY*LRKIPFELRRN*RRSVDFWCNSPFISC LKKAYCLLLGPKRP*CPWRFGLKRYSLAAQRLPP WRPHCTQ*LPGDVIAKARPDAFSYPTQGPFGMT GEGCSHIDVRTVNISKTL*FLLPNL*KGGCILV GSMYSVE*NTYSNSVYQNPFPL*QEFTSNCDLGC QKLSESPYSSLIFIYAANV*LDLPSSCSSYRHC LRDCLLLDVVMLLQDSSWLHGMPLLIWS*SSNI HPNEDIVIIPFQSLYEHGRL*PTQ*TVLGTSGNI DTVS*HH*ATGRNKFQY*DRDLFHFAPKGTDNM SPCD*AHLIKCKNMHGIFDYVTTS*N*EHTAFG NPQGVEEDQIQIREDDSF*SCKISCHP*LLG*LK FDLYISKSN**IQFVFQENQRHRNSYQVSKYES NLV*HSGLLLHYLL*EKLIDYKICATSLTLLNM Y*RLSPGLPVKLQCPSL*ISLETCFSFSDVRVPF DKLNFTKLNNSDSLKS*CGLPKGLYL*NKMQV* *LYMSMDYLDILSFGLFFKEASSRSLFS*S*YF SWKN*TGAQSQG*GLAEERNSVTSRAGSHS*GTF HK*GEIL*PIIKMNVFISK*LIFHMAKDGKMTS FYSHCK*EFAIDERSSDWHLKY*VYGISKDFLD FIFKYFLNLRGVNKLALDFQHPTLMKKA*KDPFP YK*FNPISPHFIDEETEAQIR*ELT*IHSMCRW *SWD*QHCCSPLLASLFTLSLPKNEDMQ*TEFR QDTSKLF*TL*YFVFMCLSVSPY*NVSSMGQGLH CILFIVYPQSLDQCLAHRRWQ*MLVELINKVKE RANEMKILVSYKKSYYCFLWQVPFLLHLPKDKM LICKGVIRFTWRDFMENSVQ*LLFFFFLQ*QFT* YSILQKLHEMWTQDVLYDLITCHSLTPLLPTRS SNNLSLTLQYLINFLKCK*FKALKIYHESAQHF KVTILKCL*MLYT