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Sequences

Sequence DNMT3B.kAug05

ATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAG
GCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCAT
GAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTC
TGGGGCAACCTACCCGGGATGAACAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGT
CATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCC
CGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCC
CCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCG
TGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTG
CTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCT
ACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCA
AGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTT
TTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTA
GAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTT
AGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAA
GTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCT
ACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACAT
ACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGA
AGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTT
GTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTT
TTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCT
AGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA
  • DNMT3B.u.f.27 (forward primer)
  • DNMT3B.au.1.r (reverse primer)
  • Single exon skipping

Frame 0

IEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGT
GRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAM
KVGDKRDISRFLECNPVMIDAIKVSAAHRARYF
WGNLPGMNS*RKYRQ*PPSRTRSNRGKTNFSLL
S*MAKKMFCGALSSKGSLAFLCTTQTCPTWAVVP
ARSCWEGPGACLSSDTSSPL*RTTLHVNSSSQA
PSPLGCVAEPGPRRCDS*RHPQALLFLSCVGHT
VYLSSLLLSGGRAT*LLQG*PEVPPPCAQIRPGC
LEQPNTVLIFSSPKTLKLEVGSNVAFFFSLPGS
TTQRNNG*DTKTTVPTALQYSG*C*KIIQDSYC
KSLIFENWLLLCVYRRVQL*ACSYRTFLRAQDRF
FPGQAEEKMLYMSFTRHIPLA*IQGLESARDLL
EYFPQ***FQQG*RHHHIQGYFFPHKPKGRGHS
*LNPSP*LQ*NPLGSSGRGVLSSII*AAIYFVDK
YGSSISPSSLGEECEARSLDKTPPQTHSLSRRP
TLHRHRSPDEKSATLISHLFTKLRGSDSSQGQT
YISHTFPTSER*QGKLQSSVLPLEIF*SFFIP*E
VVFRENGNSDKLHFRNAVIMVFNTFYSSYWCYF
VE*GTTLTSFVGLFIHFLKSQTSIFMFNVFIKI
FFVTGATT*QIWGKNCALFQQFLLIFRLKDDGCL
EFTLCLIKISICL*

Frame 1

LKNGAHLTW*LAEAHATISQM*IQPGKACMRVQ
AGSSSNFTTC*ITHAPRRVMTGRSSGCLRML*P
*RLATRGTSHGSWSVIQ**LMPSKFLLLTGPDTS
GATYPG*TVKESTDNNHQVELDQTGEKPTFPCC
HEWQRRCFVVH*ARKDLWLSCALHRRVQHGPWC
PPEAAGKVLERACHPTPLRPSEGLLCM*IVPARP
QAHWGVWQSQDPGGVIPEGIPRPCSSSAVWVIP
CTSVPSCSVGAEPPDSCRGSLRCRLLVHKSDLA
AWSSLTRCSFFLLLKL*NLK*VATWLFFFPFLGL
PLRETMAKIPKPQCRQLSNTQVNAEKSSKTVIA
RV*FLKTGYCSVFTDVCSCRHVATGHF*GPRIV
FSQGKQKRKCCICLLPGTFPLPKYKGWSLHGTY*
SIFHNDDDFSRDDVIITFRAIFSPTNPRAGATL
S*IPPRDCNRTLWGAQEGVC*VL*YKLPYIL*T
SMAPPYLPLP*ERSVKQGA*IRHPLKPIPSPGDL
PSTGTGPQMRSLLPSFLIFLLNSEAVTAVRDRH
TFLIPSPHLRDDRENCKARCSLWRFFNPFLFHK
KSFLGRTGIQTSCISEMLS*WFLTPFTLLTGAIL
*NKEQR*QVLWGFLYTF*NLKLLFLCLTFSLKF
FL*LEPRRNKYGEKTVPCFNSFC*FLG*KMTDA
*SLPYV*LKSVFVY

Frame 2

*RMGPI*LGDWRKPMQRSLKCESSQERPV*GYR
PALLRILPPAELLTPQGG**PAVLLDV*ECCSH
EGWRQEGHLTVPGV*SSDD*CHQSFCCSQGPIL
LGQPTRDEQLKKVQTITTKSNSIKQGKNQLFPVV
MNGKEDVLWCTELERIFGFPVHYTDVSNMGRGA
RQKLLGRSWSVPVIRHLFAPLKDYFACE*FQPG
PKPTGVCGRARTQEV*FLKASPGPALPQLCGSYR
VPQFPLAQWGQSHLTLAGVA*GAASLCTNQTWL
LGAA*HGAHFFFS*NFKT*SR*QRGFFFFPSWV
YHSEKQWLRYQNHSADSSPILRLMLKNHPRQLLQ
EFNF*KLATALCLQTCAVVGM*LQDIFKGPGSF
FPRASRRENVVYVFYPAHSPCLNTRAGVCTGPI
RVFSTMMMISAGMTSSSHSGLFFPPQTQGQGPLL
AKSLPVTAIEPSGELRKGCAEFYNISCHIFCRQ
VWLLHISLFPRRGV*SKELR*DTPSNPFPLQET
YPPQAQVPR*EVCYPHFSSFY*TQRQ*QQSGTDI
HFSYLPHI*EMTGKTAKLGAPFGDFLILFYSIR
SRF*GEREFRQAAFQKCCHNGF*HLLLFLLVLF
CRIRNNVDKFCGAFYTLFKISNFYFYV*RFH*NF
FCNWSHDVTNMGKKLCLVSTVFANF*AER*RMP
RVYLMFN*NQYLSI

Sequence DNMT3B.dAug05

GAAGGGAGAGAGCAAACAAAGGGGTCAGGAGGGAAAATAATGCACTGGCTTCCTGAGCCCCTGCAGAGGCTGAGCAGGGAGAGGGGGCCAGGGCCAGAGG
GGACAGAGGCTGGCGGCAGACGGGCCGGGACAGGCAGGTCCTAAATGGCATTGTTTGAAGGGGCCGGCTAATTGCACAGAGCAGTCTGAGCCTGAGACCC
CAGCCCTGGCCTCCCCACTCTGTCCTGGGTGCTGGCGTCTGAGCCTTCGGGACAGCCTGTCCACATGGAACCAAGTCCTGAGCCTCCAAGCTTGGAAAGC
ATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCTGCAGCGACCAGTCCTCCGACT
CGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCTACAC
ACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGC
CCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCCGGCAGGGCCGCAACCATGTGG
ACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCTCTTACCTTAC
CATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATG
GAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCA
AGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTGGGTCCAGTGGTTTGGCGATGG
CAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAA
GCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGGACCAGCTGAAGCCCATGTTGG
AGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCGAAGACGCACAGCTGACGACTC
AGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATG
GCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGC
TCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCT
GCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAG
CCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACA
CGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGG
CTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGG
AATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATC
TCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGG
TGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATG
ATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCATCAAAGAATGATAAAC
TCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCA
ACTTTTCCCTGTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAAC
ATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAAT
AGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCT
GTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACA
AATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTT
TCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCC
AAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAG
GGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCT
GCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGG
GCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCAT
ATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCC
CTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCA
GTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTT
TTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCT
TACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACG
TTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAA
AGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA
  • DNMT3B.u.f.27 (forward primer)
  • DNMT3B.au.1.r (reverse primer)
  • Single exon skipping

Frame 0

EGREQTKGSGGKIMHWLPEPLQRLSRERGPGPE
GTEAGGRRAGTGRS*MALFEGAG*LHRAV*A*DP
SPGLPTLSWVLASEPSGQPVHMEPSPEPPSLES
MKGDTRHLNGEEDAGGREDSILVNGACSDQSSD
SPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYT
QDLTGDGDGEDGDGSDTPVMPKLFRETRTRSES
PAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHV
DESPVEFPATRSLRRRATASAGTPWPSPPSSYLT
IDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGM
ESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKI
KGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDG
KFSEVSADKLVALGLFSQHFNLATFNKLVSYRK
AMYHALEKARVRAGKTFPSSPGDSLEDQLKPML
EWAHGGFKPTGIEGLKPNNTQPENKTRRRTADDS
ATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQM
ASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGG
LCQTCRDRFLELFYMYDDDGYQSYCTVCCEGREL
LLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQE
PWSCYMCLPQRCHGVLRRRKDWNVRLQAFFTSD
TGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATG
YLVLKELGIKVGKYVASEVCEESIAVGTVKHEG
NIKYVNDVRNITKKNIEEWGPFDLVIGGSPCND
LSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEG
DDRPFFWMFENVVAMKVGDKRDISRFLECNPVM
IDAIKVSAAHRARYFWGNLPGMNRPVIASKNDK
LELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQ
LFPVVMNGKEDVLWCTELERIFGFPVHYTDVSN
MGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE
*FQPGPKPTGVCGRARTQEV*FLKASPGPALPQL
CGSYRVPQFPLAQWGQSHLTLAGVA*GAASLCT
NQTWLLGAA*HGAHFFFS*NFKT*SR*QRGFFF
FPSWVYHSEKQWLRYQNHSADSSPILRLMLKNHP
RQLLQEFNF*KLATALCLQTCAVVGM*LQDIFK
GPGSFFPRASRRENVVYVFYPAHSPCLNTRAGV
CTGPIRVFSTMMMISAGMTSSSHSGLFFPPQTQG
QGPLLAKSLPVTAIEPSGELRKGCAEFYNISCH
IFCRQVWLLHISLFPRRGV*SKELR*DTPSNPF
PLQETYPPQAQVPR*EVCYPHFSSFY*TQRQ*QQ
SGTDIHFSYLPHI*EMTGKTAKLGAPFGDFLIL
FYSIRSRF*GEREFRQAAFQKCCHNGF*HLLLF
LLVLFCRIRNNVDKFCGAFYTLFKISNFYFYV*R
FH*NFFCNWSHDVTNMGKKLCLVSTVFANF*AE
R*RMPRVYLMFN*NQYLSI

Frame 1

KGESKQRGQEGK*CTGFLSPCRG*AGRGGQGQR
GQRLAADGPGQAGPKWHCLKGPANCTEQSEPET
PALASPLCPGCWRLSLRDSLSTWNQVLSLQAWKA
*RETPGISMERRTPAGGKTRSSSTGPAATSPPT
RPQSWRLSAPRRSEAEDQARDSPRGRCPVC*AT
HRT*QAMATGKMGMALTPQSCQSSSGKPGLVQKA
QLSELEITTVSPAGRGTGLPHVPPEAGRAATMW
TSPPWSSRLPGP*DGGQQHRQERHGRPLPALTL
PSTSQTTQRTHMGRPRAAVPPTPA*PRTASRGAW
SPRRWRQTVEMETVQSIRMGRSLE*GTSCGERS
RASPGGPPWWCLGRPPPSDRLCLACGGSSGLAM
ASSPRSLQTNWWHWGCSASTLIWPPSISSSPIEK
PCTMLWRKLGCELARPSPAALETHWRTS*SPCW
SGPTGASSPLGSRASNPTTRNQRTRLEDAQLTT
QPPLTTAPHPSASRQIAITTAKTEGMKIRAENKW
LQMLPTTRAAWKMAVCLVAGKTPCPSTLSLRGG
SVRHAGIASLSCFTCMMTMAISLTALCAARAES
CCFAATRAAAGVSVWSAWRCWWAQAQRPRPSFRS
PGAVTCVSRSAVMASCGAGRTGTCACRPSSPVT
RGLNMKPPSCTLPFPQPEGGPFESCHCLMASRQ
AT*SSKSWA*R*ESTSLLKCVRSPLLLEP*STRG
ISNT*TT*GTSQRKILKNGAHLTW*LAEAHATI
SQM*IQPGKACMRVQAGSSSNFTTC*ITHAPRR
VMTGRSSGCLRML*P*RLATRGTSHGSWSVIQ**
LMPSKFLLLTGPDTSGATYPG*TGP**HQRMIN
SSCRTAWNTIG*PS*RKYRQ*PPSRTRSNRGKT
NFSLLS*MAKKMFCGALSSKGSLAFLCTTQTCPT
WAVVPARSCWEGPGACLSSDTSSPL*RTTLHVN
SSSQAPSPLGCVAEPGPRRCDS*RHPQALLFLS
CVGHTVYLSSLLLSGGRAT*LLQG*PEVPPPCAQ
IRPGCLEQPNTVLIFSSPKTLKLEVGSNVAFFF
SLPGSTTQRNNG*DTKTTVPTALQYSG*C*KII
QDSYCKSLIFENWLLLCVYRRVQL*ACSYRTFLR
AQDRFFPGQAEEKMLYMSFTRHIPLA*IQGLES
ARDLLEYFPQ***FQQG*RHHHIQGYFFPHKPK
GRGHS*LNPSP*LQ*NPLGSSGRGVLSSII*AAI
YFVDKYGSSISPSSLGEECEARSLDKTPPQTHS
LSRRPTLHRHRSPDEKSATLISHLFTKLRGSDS
SQGQTYISHTFPTSER*QGKLQSSVLPLEIF*SF
FIP*EVVFRENGNSDKLHFRNAVIMVFNTFYSS
YWCYFVE*GTTLTSFVGLFIHFLKSQTSIFMFN
VFIKIFFVTGATT*QIWGKNCALFQQFLLIFRLK
DDGCLEFTLCLIKISICL*

Frame 2

RERANKGVRRENNALAS*APAEAEQGEGARARG
DRGWRQTGRDRQVLNGIV*RGRLIAQSSLSLRP
QPWPPHSVLGAGV*AFGTACPHGTKS*ASKLGK
HEGRHQASQWRGGRRREGRLDPRQRGLQRPVLRL
APNPGGYPHPGDQRPKIKLATLQEGGVQSAKLH
TGLDRRWRRGRWGWL*HPSHAKALPGNQDSFRK
PSCPNSK*QQCLQPGEAQAFPTFHPRPAGPQPCG
RVPRGVPGYQVPETAGNSIGRNAMAVPSQLLPY
HRPHRRHRGHTWDAPEQQYPLRPPSPGQPAGGH
GVPAGGGRQWRWRQFRVSGWEGVWNRGPRVGKDQ
GLLLVARHGGVLEGHLQATGYVWHAVGPVVWRW
QVLRGLCRQTGGTGAVQPAL*FGHLQ*ARLLSK
SHVPCSGES*GASWQDLPQQPWRLIGGPAEAHVG
VGPRGLQAHWDRGPQTQQHATREQDSKTHS*RL
SHL*LLPRTQAPQDKLL*QRQRPRG*RSEPRTN
GFRCCQQQEQPGRWLFVLWQEKPRVLPPSL*GGA
LSDMPGSLP*AVLHV**RWLSVLLHCVLRGPRA
AALQQHELLPVFLCGVPGGAGGHRHSGRGQASG
ALELLHVSPAALSWRPAAPEGLERAPAGLLHQ*H
GA*I*SPQAVPCHSRSPKAAHSSPVIV*WHRDR
LPSPQRVGHKGRKVRRF*SV*GVHCCWNREARG
EYQIRERREEHHKEKY*RMGPI*LGDWRKPMQRS
LKCESSQERPV*GYRPALLRILPPAELLTPQGG
**PAVLLDV*ECCSHEGWRQEGHLTVPGV*SSD
D*CHQSFCCSQGPILLGQPTRDEQARDSIKE**T
RAAGLLGIQ*DSQVKESTDNNHQVELDQTGEKP
TFPCCHEWQRRCFVVH*ARKDLWLSCALHRRVQ
HGPWCPPEAAGKVLERACHPTPLRPSEGLLCM*I
VPARPQAHWGVWQSQDPGGVIPEGIPRPCSSSA
VWVIPCTSVPSCSVGAEPPDSCRGSLRCRLLVH
KSDLAAWSSLTRCSFFLLLKL*NLK*VATWLFFF
PFLGLPLRETMAKIPKPQCRQLSNTQVNAEKSS
KTVIARV*FLKTGYCSVFTDVCSCRHVATGHF*
GPRIVFSQGKQKRKCCICLLPGTFPLPKYKGWSL
HGTY*SIFHNDDDFSRDDVIITFRAIFSPTNPR
AGATLS*IPPRDCNRTLWGAQEGVC*VL*YKLP
YIL*TSMAPPYLPLP*ERSVKQGA*IRHPLKPIP
SPGDLPSTGTGPQMRSLLPSFLIFLLNSEAVTA
VRDRHTFLIPSPHLRDDRENCKARCSLWRFFNP
FLFHKKSFLGRTGIQTSCISEMLS*WFLTPFTLL
TGAIL*NKEQR*QVLWGFLYTF*NLKLLFLCLT
FSLKFFL*LEPRRNKYGEKTVPCFNSFC*FLG*
KMTDA*SLPYV*LKSVFVY

Sequence DNMT3B.bAug05

ACCCACTCCCGCTGCCCCGTCCGGCCCGCGCCGCTTCCTCGCAGCAGCTGCTCCCGGCTCCGCGGCCGCAGCCCGCGTGGACGCTCCGAGCGCCCCCCGA
CGGACGGGACCGGCTCCCTGGCGGTCGGGCGAGCGGGCGGCAACGCTGCCCGGCCGGCAGCGCTGGGGTTAAGTGGCCCAAGTAAACCTAGCTCGGCGAT
CGGCGCCGGAGATTCGCGAGCCCAGCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCGCCCAGCCGCGCCCCAG
CCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCT
GCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGT
GTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAA
ACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCC
GGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTC
CCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAG
GACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGG
ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTG
GGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAAT
AAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGG
ACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCG
AAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAA
GATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCC
ACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGT
GTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCG
GCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGC
AGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATT
GTTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTT
GGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTG
GCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAA
TTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTC
CTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGA
TAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTC
GATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTG
CACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGA
AGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCC
AGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGA
GGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGT
AGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAG
GTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATG
TAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCC
TAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATT
TTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAG
TTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAG
ACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTA
AACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCC
TTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTT
TTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAA
CTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTT
TTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA
  • DNMT3B.u.f.27 (forward primer)
  • DNMT3B.au.1.r (reverse primer)
  • Single exon skipping

Frame 0

THSRCPVRPAPLPRSSCSRLRGRSPRGRSERPP
TDGTGSLAVGRAGGNAARPAALGLSGPSKPSSAI
GAGDSRAQRPARPPAGLPPASPDPRLRRPAAPQ
PALRQESMKGDTRHLNGEEDAGGREDSILVNGA
CSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREV
SSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE
TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRG
RQGRNHVDESPVEFPATRSLRRRATASAGTPWPS
PPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQ
DSQQGGMESPQVEADSGDGDSSEYQDGKEFGIG
DLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRW
VQWFGDGKFSEVSADKLVALGLFSQHFNLATFN
KLVSYRKAMYHALEKARVRAGKTFPSSPGDSLE
DQLKPMLEWAHGGFKPTGIEGLKPNNTQPENKTR
RRTADDSATSDYCPAPKRLKTNCYNNGKDRGDE
DQSREQMASDVANNKSSLEDGCLSCGRKNPVSF
HPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTV
CCEGRELLLCSNTSCCRCFCVECLEVLVGTGTA
AEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRL
QAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSL
FDGIATGYLVLKELGIKVGKYVASEVCEESIAV
GTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVI
GGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLN
YSRPKEGDDRPFFWMFENVVAMKVGDKRDISRF
LECNPVMIDAIKVSAAHRARYFWGNLPGMNRPV
IASKNDKLELQDCLEYNRIAKLKKVQTITTKSNS
IKQGKNQLFPVVMNGKEDVLWCTELERIFGFPV
HYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPL
KDYFACE*FQPGPKPTGVCGRARTQEV*FLKASP
GPALPQLCGSYRVPQFPLAQWGQSHLTLAGVA*
GAASLCTNQTWLLGAA*HGAHFFFS*NFKT*SR
*QRGFFFFPSWVYHSEKQWLRYQNHSADSSPILR
LMLKNHPRQLLQEFNF*KLATALCLQTCAVVGM
*LQDIFKGPGSFFPRASRRENVVYVFYPAHSPC
LNTRAGVCTGPIRVFSTMMMISAGMTSSSHSGLF
FPPQTQGQGPLLAKSLPVTAIEPSGELRKGCAE
FYNISCHIFCRQVWLLHISLFPRRGV*SKELR*
DTPSNPFPLQETYPPQAQVPR*EVCYPHFSSFY*
TQRQ*QQSGTDIHFSYLPHI*EMTGKTAKLGAP
FGDFLILFYSIRSRF*GEREFRQAAFQKCCHNG
F*HLLLFLLVLFCRIRNNVDKFCGAFYTLFKISN
FYFYV*RFH*NFFCNWSHDVTNMGKKLCLVSTV
FANF*AER*RMPRVYLMFN*NQYLSI

Frame 1

PTPAAPSGPRRFLAAAAPGSAAAARVDAPSAPR
RTGPAPWRSGERAATLPGRQRWG*VAQVNLARR
SAPEIREPSALHGRQPASRQPAPTRGSAAQPRPS
QPCGRKA*RETPGISMERRTPAGGKTRSSSTGP
AATSPPTRPQSWRLSAPRRSEAEDQARDSPRGR
CPVC*ATHRT*QAMATGKMGMALTPQSCQSSSGK
PGLVQKAQLSELEITTVSPAGRGTGLPHVPPEA
GRAATMWTSPPWSSRLPGP*DGGQQHRQERHGR
PLPALTLPSTSQTTQRTHMGRPRAAVPPTPA*PR
TASRGAWSPRRWRQTVEMETVQSIRMGRSLE*G
TSCGERSRASPGGPPWWCLGRPPPSDRLCLACG
GSSGLAMASSPRSLQTNWWHWGCSASTLIWPPSI
SSSPIEKPCTMLWRKLGCELARPSPAALETHWR
TS*SPCWSGPTGASSPLGSRASNPTTRNQRTRL
EDAQLTTQPPLTTAPHPSASRQIAITTAKTEGMK
IRAENKWLQMLPTTRAAWKMAVCLVAGKTPCPS
TLSLRGGSVRHAGIASLSCFTCMMTMAISLTAL
CAARAESCCFAATRAAAGVSVWSAWRCWWAQAQR
PRPSFRSPGAVTCVSRSAVMASCGAGRTGTCAC
RPSSPVTRGLNMKPPSCTLPFPQPEGGPFESCH
CLMASRQAT*SSKSWA*R*ESTSLLKCVRSPLLL
EP*STRGISNT*TT*GTSQRKILKNGAHLTW*L
AEAHATISQM*IQPGKACMRVQAGSSSNFTTC*
ITHAPRRVMTGRSSGCLRML*P*RLATRGTSHGS
WSVIQ**LMPSKFLLLTGPDTSGATYPG*TGP*
*HQRMINSSCRTAWNTIG*PS*RKYRQ*PPSRT
RSNRGKTNFSLLS*MAKKMFCGALSSKGSLAFLC
TTQTCPTWAVVPARSCWEGPGACLSSDTSSPL*
RTTLHVNSSSQAPSPLGCVAEPGPRRCDS*RHP
QALLFLSCVGHTVYLSSLLLSGGRAT*LLQG*PE
VPPPCAQIRPGCLEQPNTVLIFSSPKTLKLEVG
SNVAFFFSLPGSTTQRNNG*DTKTTVPTALQYS
G*C*KIIQDSYCKSLIFENWLLLCVYRRVQL*AC
SYRTFLRAQDRFFPGQAEEKMLYMSFTRHIPLA
*IQGLESARDLLEYFPQ***FQQG*RHHHIQGY
FFPHKPKGRGHS*LNPSP*LQ*NPLGSSGRGVLS
SII*AAIYFVDKYGSSISPSSLGEECEARSLDK
TPPQTHSLSRRPTLHRHRSPDEKSATLISHLFT
KLRGSDSSQGQTYISHTFPTSER*QGKLQSSVLP
LEIF*SFFIP*EVVFRENGNSDKLHFRNAVIMV
FNTFYSSYWCYFVE*GTTLTSFVGLFIHFLKSQ
TSIFMFNVFIKIFFVTGATT*QIWGKNCALFQQF
LLIFRLKDDGCLEFTLCLIKISICL*

Frame 2

PLPLPRPARAASSQQLLPAPRPQPAWTLRAPPD
GRDRLPGGRASGRQRCPAGSAGVKWPK*T*LGD
RRRRFASPAPCTAASRPPASQPRPAAPPPSRAP
ASPAAGKHEGRHQASQWRGGRRREGRLDPRQRGL
QRPVLRLAPNPGGYPHPGDQRPKIKLATLQEGG
VQSAKLHTGLDRRWRRGRWGWL*HPSHAKALPG
NQDSFRKPSCPNSK*QQCLQPGEAQAFPTFHPRP
AGPQPCGRVPRGVPGYQVPETAGNSIGRNAMAV
PSQLLPYHRPHRRHRGHTWDAPEQQYPLRPPSP
GQPAGGHGVPAGGGRQWRWRQFRVSGWEGVWNRG
PRVGKDQGLLLVARHGGVLEGHLQATGYVWHAV
GPVVWRWQVLRGLCRQTGGTGAVQPAL*FGHLQ
*ARLLSKSHVPCSGES*GASWQDLPQQPWRLIGG
PAEAHVGVGPRGLQAHWDRGPQTQQHATREQDS
KTHS*RLSHL*LLPRTQAPQDKLL*QRQRPRG*
RSEPRTNGFRCCQQQEQPGRWLFVLWQEKPRVLP
PSL*GGALSDMPGSLP*AVLHV**RWLSVLLHC
VLRGPRAAALQQHELLPVFLCGVPGGAGGHRHS
GRGQASGALELLHVSPAALSWRPAAPEGLERAPA
GLLHQ*HGA*I*SPQAVPCHSRSPKAAHSSPVI
V*WHRDRLPSPQRVGHKGRKVRRF*SV*GVHCC
WNREARGEYQIRERREEHHKEKY*RMGPI*LGDW
RKPMQRSLKCESSQERPV*GYRPALLRILPPAE
LLTPQGG**PAVLLDV*ECCSHEGWRQEGHLTV
PGV*SSDD*CHQSFCCSQGPILLGQPTRDEQARD
SIKE**TRAAGLLGIQ*DSQVKESTDNNHQVEL
DQTGEKPTFPCCHEWQRRCFVVH*ARKDLWLSC
ALHRRVQHGPWCPPEAAGKVLERACHPTPLRPSE
GLLCM*IVPARPQAHWGVWQSQDPGGVIPEGIP
RPCSSSAVWVIPCTSVPSCSVGAEPPDSCRGSL
RCRLLVHKSDLAAWSSLTRCSFFLLLKL*NLK*V
ATWLFFFPFLGLPLRETMAKIPKPQCRQLSNTQ
VNAEKSSKTVIARV*FLKTGYCSVFTDVCSCRH
VATGHF*GPRIVFSQGKQKRKCCICLLPGTFPLP
KYKGWSLHGTY*SIFHNDDDFSRDDVIITFRAI
FSPTNPRAGATLS*IPPRDCNRTLWGAQEGVC*
VL*YKLPYIL*TSMAPPYLPLP*ERSVKQGA*IR
HPLKPIPSPGDLPSTGTGPQMRSLLPSFLIFLL
NSEAVTAVRDRHTFLIPSPHLRDDRENCKARCS
LWRFFNPFLFHKKSFLGRTGIQTSCISEMLS*WF
LTPFTLLTGAIL*NKEQR*QVLWGFLYTF*NLK
LLFLCLTFSLKFFL*LEPRRNKYGEKTVPCFNS
FC*FLG*KMTDA*SLPYV*LKSVFVY

Sequence DNMT3B.aAug05

ACCCACTCCCGCTGCCCCGTCCGGCCCGCGCCGCTTCCTCGCAGCAGCTGCTCCCGGCTCCGCGGCCGCAGCCCGCGTGGACGCTCCGAGCGCCCCCCGA
CGGACGGGACCGGCTCCCTGGCGGTCGGGCGAGCGGGCGGCAACGCTGCCCGGCCGGCAGCGCTGGGGTTAAGTGGCCCAAGTAAACCTAGCTCGGCGAT
CGGCGCCGGAGATTCGCGAGCCCAGCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCGCCCAGCCGCGCCCCAG
CCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCT
GCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGT
GTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAA
ACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCC
GGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTC
CCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAG
GACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGG
ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTG
GGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAAT
AAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGG
ACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGTGGTTAATAAGTC
GAAGGTGCGTCGTGCAGGCAGTAGGAAATTAGAATCAAGGAAATACGAGAACAAGACTCGAAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGC
CCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACA
ACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGA
TCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACG
AGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGT
GTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGC
CCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAG
TTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACG
ACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGC
CAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTC
TGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTT
CTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTT
GGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGTCATG
AATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCC
AGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAA
GCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTA
CCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTG
GAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCA
CTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAG
TTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTC
CCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGT
ATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCT
AAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTAT
GGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCC
TCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACAT
TTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTC
GTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAG
AATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTT
TGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAG
TTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA
  • DNMT3B.u.f.27 (forward primer)
  • DNMT3B.au.1.r (reverse primer)
  • Single exon skipping

Frame 0

THSRCPVRPAPLPRSSCSRLRGRSPRGRSERPP
TDGTGSLAVGRAGGNAARPAALGLSGPSKPSSAI
GAGDSRAQRPARPPAGLPPASPDPRLRRPAAPQ
PALRQESMKGDTRHLNGEEDAGGREDSILVNGA
CSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREV
SSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE
TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRG
RQGRNHVDESPVEFPATRSLRRRATASAGTPWPS
PPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQ
DSQQGGMESPQVEADSGDGDSSEYQDGKEFGIG
DLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRW
VQWFGDGKFSEVSADKLVALGLFSQHFNLATFN
KLVSYRKAMYHALEKARVRAGKTFPSSPGDSLE
DQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKS
KVRRAGSRKLESRKYENKTRRRTADDSATSDYC
PAPKRLKTNCYNNGKDRGDEDQSREQMASDVAN
NKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRD
RFLELFYMYDDDGYQSYCTVCCEGRELLLCSNT
SCCRCFCVECLEVLVGTGTAAEAKLQEPWSCYM
CLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEA
PKLYPAIPAARRRPIRVLSLFDGIATGYLVLKE
LGIKVGKYVASEVCEESIAVGTVKHEGNIKYVN
DVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPA
RKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFF
WMFENVVAMKVGDKRDISRFLECNPVMIDAIKV
SAAHRARYFWGNLPGMNRPVIASKNDKLELQDCL
EYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVM
NGKEDVLWCTELERIFGFPVHYTDVSNMGRGAR
QKLLGRSWSVPVIRHLFAPLKDYFACE*FQPGPK
PTGVCGRARTQEV*FLKASPGPALPQLCGSYRV
PQFPLAQWGQSHLTLAGVA*GAASLCTNQTWLL
GAA*HGAHFFFS*NFKT*SR*QRGFFFFPSWVYH
SEKQWLRYQNHSADSSPILRLMLKNHPRQLLQE
FNF*KLATALCLQTCAVVGM*LQDIFKGPGSFF
PRASRRENVVYVFYPAHSPCLNTRAGVCTGPIRV
FSTMMMISAGMTSSSHSGLFFPPQTQGQGPLLA
KSLPVTAIEPSGELRKGCAEFYNISCHIFCRQV
WLLHISLFPRRGV*SKELR*DTPSNPFPLQETYP
PQAQVPR*EVCYPHFSSFY*TQRQ*QQSGTDIH
FSYLPHI*EMTGKTAKLGAPFGDFLILFYSIRS
RF*GEREFRQAAFQKCCHNGF*HLLLFLLVLFCR
IRNNVDKFCGAFYTLFKISNFYFYV*RFH*NFF
CNWSHDVTNMGKKLCLVSTVFANF*AER*RMPR
VYLMFN*NQYLSI

Frame 1

PTPAAPSGPRRFLAAAAPGSAAAARVDAPSAPR
RTGPAPWRSGERAATLPGRQRWG*VAQVNLARR
SAPEIREPSALHGRQPASRQPAPTRGSAAQPRPS
QPCGRKA*RETPGISMERRTPAGGKTRSSSTGP
AATSPPTRPQSWRLSAPRRSEAEDQARDSPRGR
CPVC*ATHRT*QAMATGKMGMALTPQSCQSSSGK
PGLVQKAQLSELEITTVSPAGRGTGLPHVPPEA
GRAATMWTSPPWSSRLPGP*DGGQQHRQERHGR
PLPALTLPSTSQTTQRTHMGRPRAAVPPTPA*PR
TASRGAWSPRRWRQTVEMETVQSIRMGRSLE*G
TSCGERSRASPGGPPWWCLGRPPPSDRLCLACG
GSSGLAMASSPRSLQTNWWHWGCSASTLIWPPSI
SSSPIEKPCTMLWRKLGCELARPSPAALETHWR
TS*SPCWSGPTGASSPLGSRASNPTTRNQWLIS
RRCVVQAVGN*NQGNTRTRLEDAQLTTQPPLTTA
PHPSASRQIAITTAKTEGMKIRAENKWLQMLPT
TRAAWKMAVCLVAGKTPCPSTLSLRGGSVRHAG
IASLSCFTCMMTMAISLTALCAARAESCCFAATR
AAAGVSVWSAWRCWWAQAQRPRPSFRSPGAVTC
VSRSAVMASCGAGRTGTCACRPSSPVTRGLNMK
PPSCTLPFPQPEGGPFESCHCLMASRQAT*SSKS
WA*R*ESTSLLKCVRSPLLLEP*STRGISNT*T
T*GTSQRKILKNGAHLTW*LAEAHATISQM*IQ
PGKACMRVQAGSSSNFTTC*ITHAPRRVMTGRSS
GCLRML*P*RLATRGTSHGSWSVIQ**LMPSKF
LLLTGPDTSGATYPG*TGP**HQRMINSSCRTA
WNTIG*PS*RKYRQ*PPSRTRSNRGKTNFSLLS*
MAKKMFCGALSSKGSLAFLCTTQTCPTWAVVPA
RSCWEGPGACLSSDTSSPL*RTTLHVNSSSQAP
SPLGCVAEPGPRRCDS*RHPQALLFLSCVGHTVY
LSSLLLSGGRAT*LLQG*PEVPPPCAQIRPGCL
EQPNTVLIFSSPKTLKLEVGSNVAFFFSLPGST
TQRNNG*DTKTTVPTALQYSG*C*KIIQDSYCKS
LIFENWLLLCVYRRVQL*ACSYRTFLRAQDRFF
PGQAEEKMLYMSFTRHIPLA*IQGLESARDLLE
YFPQ***FQQG*RHHHIQGYFFPHKPKGRGHS*L
NPSP*LQ*NPLGSSGRGVLSSII*AAIYFVDKY
GSSISPSSLGEECEARSLDKTPPQTHSLSRRPT
LHRHRSPDEKSATLISHLFTKLRGSDSSQGQTYI
SHTFPTSER*QGKLQSSVLPLEIF*SFFIP*EV
VFRENGNSDKLHFRNAVIMVFNTFYSSYWCYFV
E*GTTLTSFVGLFIHFLKSQTSIFMFNVFIKIFF
VTGATT*QIWGKNCALFQQFLLIFRLKDDGCLE
FTLCLIKISICL*

Frame 2

PLPLPRPARAASSQQLLPAPRPQPAWTLRAPPD
GRDRLPGGRASGRQRCPAGSAGVKWPK*T*LGD
RRRRFASPAPCTAASRPPASQPRPAAPPPSRAP
ASPAAGKHEGRHQASQWRGGRRREGRLDPRQRGL
QRPVLRLAPNPGGYPHPGDQRPKIKLATLQEGG
VQSAKLHTGLDRRWRRGRWGWL*HPSHAKALPG
NQDSFRKPSCPNSK*QQCLQPGEAQAFPTFHPRP
AGPQPCGRVPRGVPGYQVPETAGNSIGRNAMAV
PSQLLPYHRPHRRHRGHTWDAPEQQYPLRPPSP
GQPAGGHGVPAGGGRQWRWRQFRVSGWEGVWNRG
PRVGKDQGLLLVARHGGVLEGHLQATGYVWHAV
GPVVWRWQVLRGLCRQTGGTGAVQPAL*FGHLQ
*ARLLSKSHVPCSGES*GASWQDLPQQPWRLIGG
PAEAHVGVGPRGLQAHWDRGPQTQQHATSG**V
EGASCRQ*EIRIKEIREQDSKTHS*RLSHL*LL
PRTQAPQDKLL*QRQRPRG*RSEPRTNGFRCCQQ
QEQPGRWLFVLWQEKPRVLPPSL*GGALSDMPG
SLP*AVLHV**RWLSVLLHCVLRGPRAAALQQH
ELLPVFLCGVPGGAGGHRHSGRGQASGALELLHV
SPAALSWRPAAPEGLERAPAGLLHQ*HGA*I*S
PQAVPCHSRSPKAAHSSPVIV*WHRDRLPSPQR
VGHKGRKVRRF*SV*GVHCCWNREARGEYQIRER
REEHHKEKY*RMGPI*LGDWRKPMQRSLKCESS
QERPV*GYRPALLRILPPAELLTPQGG**PAVL
LDV*ECCSHEGWRQEGHLTVPGV*SSDD*CHQSF
CCSQGPILLGQPTRDEQARDSIKE**TRAAGLL
GIQ*DSQVKESTDNNHQVELDQTGEKPTFPCCH
EWQRRCFVVH*ARKDLWLSCALHRRVQHGPWCPP
EAAGKVLERACHPTPLRPSEGLLCM*IVPARPQ
AHWGVWQSQDPGGVIPEGIPRPCSSSAVWVIPC
TSVPSCSVGAEPPDSCRGSLRCRLLVHKSDLAAW
SSLTRCSFFLLLKL*NLK*VATWLFFFPFLGLP
LRETMAKIPKPQCRQLSNTQVNAEKSSKTVIAR
V*FLKTGYCSVFTDVCSCRHVATGHF*GPRIVFS
QGKQKRKCCICLLPGTFPLPKYKGWSLHGTY*S
IFHNDDDFSRDDVIITFRAIFSPTNPRAGATLS
*IPPRDCNRTLWGAQEGVC*VL*YKLPYIL*TSM
APPYLPLP*ERSVKQGA*IRHPLKPIPSPGDLP
STGTGPQMRSLLPSFLIFLLNSEAVTAVRDRHT
FLIPSPHLRDDRENCKARCSLWRFFNPFLFHKKS
FLGRTGIQTSCISEMLS*WFLTPFTLLTGAIL*
NKEQR*QVLWGFLYTF*NLKLLFLCLTFSLKFF
L*LEPRRNKYGEKTVPCFNSFC*FLG*KMTDA*S
LPYV*LKSVFVY

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