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Sequences

Sequence BTC.bAug05

BTC.F2 (forward primer)
BTC.R2 (reverse primer)
Single exon skipping

Frame 0

LVRGQPEYVPGCGYGCGAIWVGAAWRLPGPSPA PLWRGVCRRRGDK*EAPSSRRHSRSRPASRPGCF PAL*PGCQPGK*FRFLLPPGLVSRHPLRRPRPG KGAKKEGDLSRGCPARQSGVDGPGRPVQRRQLP ATAPGPCPGSSDPSLCGGRWEFHQKS*N*WPPLW RP*GKLCSYHHTIKAERPLL*VPQAIQALLHQR EMPLRGGRADALLCPSSETS*KKEERRRNGNSG *RYNSYQ*RY*RDKYCLKGYEVTSRLVASCKVPC SFENGQNVSQENS**T*ILNNVFTFYLQL*FVL LFFNKNINYMYIV**LGKKQLVR*QQQKGNFNN LSLKYCHQDY*SN

Frame 1

WSEGSPNMSLGVGMGVGQFGWEQRGGSQDQVLR LFGGVCAGGGGINRRLPPPGDIHGAGRPPALGV SLPCSQGASLGSSFVSFCLRD*FPGTLSGARGPG RGRRRRETCLGAARPGRAGLMDRAARCSGASSL PLLLALALGLVILHCVVADGNSTRSPETNGLLC GDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKG RCRFVVAEQTPSCVPLRKRRKRKKKEEEMETLG KDITPINEDIEETNIA*KAMKLPPGWWQAAKCL AHLKMDRMCLRKTASRHEF*IMYLLFICNFSLCY YFLIRTLIICILSSNWEKSNWLGSNNRREISIT FHLSIVTRITSQ

Frame 2

GPRAARICPWVWVWVWGNLGGSSVEAPRTKSCA SLAGCVQEEGG*IGGSLLPATFTEPAGLPPWVF PCLVARVPAWEVVSFPSASGISFQAPSQAPEAR EGGEEGGRLV*GLPGPAERG*WTGPPGAAAPAPC HCSWPLPWV**SFTVWWQMGIPPEVLKLMASSV ETLRKTVQLPPHNQSGKATSLGAPSNTSITASK GDAASWWPSRRPPVSLFGNVVKERRKKKKWKLWV KI*LLSMKILKRQILLKRL*SYLQVGGKLQSAL LI*KWTECVSGKQLVDMNFK*CIYFLFATLVCV IIF**EH*LYVYCLVIGKKATG*VATTEGKFQ*P FT*VLSPGLLVK

Sequence BTC.aAug05

CTGGTCCGAGGGCAGCCCGAATATGTCCCTGGGTGTGGGTATGGGTGTGGGGCAATTTGGGTGGGAGCAGCGTGGAGGCTCCCAGGACCAAGTCCTGCGC CTCTTTGGCGGGGTGTGTGCAGGAGGAGGGGGGATAAATAGGAGGCTCCCTCCTCCCGGCGACATTCACGGAGCCGGCCGGCCTCCCGCCCTGGGTGTTT CCCTGCCTTGTAGCCAGGGTGCCAGCCTGGGAAGTAGTTTCGTTTCCTTCTGCCTCCGGGATTAGTTTCCAGGCACCCTCTCAGGCGCCCGAGGCCCGGG AAGGGGGCGAAGAAGGAGGGAGACTTGTCTAGGGGCTGCCCGGCCCGGCAGAGCGGGGTTGATGGACCGGGCCGCCCGGTGCAGCGGCGCCAGCTCCCTG CCACTGCTCCTGGCCCTTGCCCTGGGTCTAGTGATCCTTCACTGTGTGGTGGCAGATGGGAATTCCACCAGAAGTCCTGAAACTAATGGCCTCCTCTGTG GAGACCCTGAGGAAAACTGTGCAGCTACCACCACACAATCAAAGCGGAAAGGCCACTTCTCTAGGTGCCCCAAGCAATACAAGCATTACTGCATCAAAGG GAGATGCCGCTTCGTGGTGGCCGAGCAGACGCCCTCCTGTGTCTGTGATGAAGGCTACATTGGAGCAAGGTGTGAGAGAGTTGACTTGTTTTACCTAAGA GGAGACAGAGGACAGATTCTGGTGATTTGTTTGATAGCAGTTATGGTAGTTTTTATTATTTTGGTCATCGGTGTCTGCACATGCTGTCACCCTCTTCGGA AACGTCGTAAAAGAAAGAAGAAAGAAGAAGAAATGGAAACTCTGGGTAAAGATATAACTCCTATCAATGAAGATATTGAAGAGACAAATATTGCTTAAAA GGCTATGAAGTTACCTCCAGGTTGGTGGCAAGCTGCAAAGTGCCTTGCTCATTTGAAAATGGACAGAATGTGTCTCAGGAAAACAGCTAGTAGACATGAA TTTTAAATAATGTATTTACTTTTTATTTGCAACTTTAGTTTGTGTTATTATTTTTTAATAAGAACATTAATTATATGTATATTGTCTAGTAATTGGGAAA AAAGCAACTGGTTAGGTAGCAACAACAGAAGGGAAATTTCAATAACCTTTCACTTAAGTATTGTCACCAGGATTACTAGTCAAACAAAAAAGAAAAGTAG AAAGGAGGTTAGGTCTTAGGAATTGAATTAATAATAAAGCTACCATTTATCAAGCATTTACCATGTGCTAATAAGTTTGAAATATATTATTTCCTTTATT CCTTTCAGCAATCCATGAGATAGCTATTATAATCCTCATTTCCTACATATGGAAACAGGGCCAAAGAAGTCAAGTCAAATAATCTAATCCAGATTTAGAG AGTTGGAGATTGGTAGAGACAGACTAGAAATCAAGATTTATCTAAATCAAAATCTATGCTCTTAACCATCATTTTTGTTAGTCTGAATCGGTGCAACCTA ACTCAATAATATAACCCAAGGTTTTATACTGAACTGTGTTTTGGTATGATTGTGAGATGTCAAGACCGTCTTCCCACAGGCACACAGAATGAGAAATCCT GGCCTATATTGCTGTTACCATCATGCTTTCTATAGAATTGGCTTTTATTATGTTTCTTTGCTCCTTTATAAATTCTCAATGATGTGACTACATTTTTACA TTATTAAAGCAAATAGATTGGCCCCATAACTAGGGAAATGAATAGAGATGGAAAAGTAAATGCTTGGCATTCCTTAAGCCCTGGGTAAGTGTTTATGCTC AATAAAATATAAATAATATTACTGAAAGAGGCTGTTTATATGGGAGTAATGGTCAGTGACAACACTGGAGAGACCAGGTAGGGACCGGTTGTGACCATAT CCTCATTTATGTTCTTGATGACAGCATATATGATTGCTCTCAGGTAAATTTTGATGCAGTAAATTCATGACCATAACATCTATTACTTGGGACCATCTTG CCAAGTGCTCCTTTACTCCATGAAAAAACAATGCTTTAATGTTTCTTTTTTCCTGGACTGAGTTTGGCTGACCAACAAAATCTCTCAAGCCATTTTGTTT TGATCCTAAAATATCAATGGTGGCTCTCGAAATTTGGTAGATCTGATATCAAGAAAGAATTTCAAAGATTTTTATATCTTTTCAAAATGTATTTTATAGT TGCTTTGCATACACTAAATAACTTTGTTTATGTAAACTGTCAATATTATCATGTAATAAAATAAATTTATTTTTAAAACAAAGTAAACGTGTGGTTACTT GGGAATTTTTTATTTTACCATGAATTACTAATGAAAATAAATTTGAACACTATTGCTTTTAAACACCTTATGCTTTGTAAACCCTATCCATTAGTGTACT TGAAAGAATCCATCCTGACCATGTGTTTTCTTTAGGAACTAAAGCATCTGGGAGTGTGTTGGACATCTATGGGACTGTCTTGCCAATATCCTGGCCATAT TTTTGAACTCCACCTCCAGCTGCATTTGATTGGTCTGAGGTGGGCACTTAATAAACTTGGGC

BTC.F2 (forward primer)
BTC.R2 (reverse primer)
Single exon skipping

Frame 0

LVRGQPEYVPGCGYGCGAIWVGAAWRLPGPSPA PLWRGVCRRRGDK*EAPSSRRHSRSRPASRPGCF PAL*PGCQPGK*FRFLLPPGLVSRHPLRRPRPG KGAKKEGDLSRGCPARQSGVDGPGRPVQRRQLP ATAPGPCPGSSDPSLCGGRWEFHQKS*N*WPPLW RP*GKLCSYHHTIKAERPLL*VPQAIQALLHQR EMPLRGGRADALLCL**RLHWSKV*ES*LVLPK RRQRTDSGDLFDSSYGSFYYFGHRCLHMLSPSSE TS*KKEERRRNGNSG*RYNSYQ*RY*RDKYCLK GYEVTSRLVASCKVPCSFENGQNVSQENS**T* ILNNVFTFYLQL*FVLLFFNKNINYMYIV**LGK KQLVR*QQQKGNFNNLSLKYCHQDY*SNKKEK* KGG*VLGIELIIKLPFIKHLPCANKFEIYYFLY SFQQSMR*LL*SSFPTYGNRAKEVKSNNLIQI*R VGDW*RQTRNQDLSKSKSMLLTIIFVSLNRCNL TQ*YNPRFYTELCFGMIVRCQDRLPTGTQNEKS WPILLLPSCFL*NWLLLCFFAPL*ILNDVTTFLH Y*SK*IGPITREMNRDGKVNAWHSLSPG*VFML NKI*IILLKEAVYMGVMVSDNTGETR*GPVVTI SSFMFLMTAYMIALR*ILMQ*IHDHNIYYLGPSC QVLLYSMKKQCFNVSFFLD*VWLTNKISQAILF *S*NINGGSRNLVDLISRKNFKDFYIFSKCIL* LLCIH*ITLFM*TVNIIM**NKFIFKTK*TCGYL GIFYFTMNY**K*I*TLLLLNTLCFVNPIH*CT *KNPS*PCVFFRN*SIWECVGHLWDCLANILAI FLNSTSSCI*LV*GGHLINLG

Frame 1

WSEGSPNMSLGVGMGVGQFGWEQRGGSQDQVLR LFGGVCAGGGGINRRLPPPGDIHGAGRPPALGV SLPCSQGASLGSSFVSFCLRD*FPGTLSGARGPG RGRRRRETCLGAARPGRAGLMDRAARCSGASSL PLLLALALGLVILHCVVADGNSTRSPETNGLLC GDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKG RCRFVVAEQTPSCVCDEGYIGARCERVDLFYLR GDRGQILVICLIAVMVVFIILVIGVCTCCHPLR KRRKRKKKEEEMETLGKDITPINEDIEETNIA*K AMKLPPGWWQAAKCLAHLKMDRMCLRKTASRHE F*IMYLLFICNFSLCYYFLIRTLIICILSSNWE KSNWLGSNNRREISITFHLSIVTRITSQTKKKSR KEVRS*ELN***SYHLSSIYHVLISLKYIISFI PFSNP*DSYYNPHFLHMETGPKKSSQII*SRFR ELEIGRDRLEIKIYLNQNLCS*PSFLLV*IGAT* LNNITQGFILNCVLV*L*DVKTVFPQAHRMRNP GLYCCYHHAFYRIGFYYVSLLLYKFSMM*LHFY IIKANRLAP*LGK*IEMEK*MLGIP*ALGKCLCS IKYK*YY*KRLFIWE*WSVTTLERPGRDRL*PY PHLCS**QHI*LLSGKF*CSKFMTITSITWDHL AKCSFTP*KNNALMFLFSWTEFG*PTKSLKPFCF DPKISMVALEIW*I*YQERISKIFISFQNVFYS CFAYTK*LCLCKLSILSCNKINLFLKQSKRVVT WEFFILP*ITNENKFEHYCF*TPYAL*TLSISVL ERIHPDHVFSLGTKASGSVLDIYGTVLPISWPY F*TPPPAAFDWSEVGT**TW

Frame 2

GPRAARICPWVWVWVWGNLGGSSVEAPRTKSCA SLAGCVQEEGG*IGGSLLPATFTEPAGLPPWVF PCLVARVPAWEVVSFPSASGISFQAPSQAPEAR EGGEEGGRLV*GLPGPAERG*WTGPPGAAAPAPC HCSWPLPWV**SFTVWWQMGIPPEVLKLMASSV ETLRKTVQLPPHNQSGKATSLGAPSNTSITASK GDAASWWPSRRPPVSVMKATLEQGVRELTCFT*E ETEDRFW*FV**QLW*FLLFWSSVSAHAVTLFG NVVKERRKKKKWKLWVKI*LLSMKILKRQILLK RL*SYLQVGGKLQSALLI*KWTECVSGKQLVDMN FK*CIYFLFATLVCVIIF**EH*LYVYCLVIGK KATG*VATTEGKFQ*PFT*VLSPGLLVKQKRKV ERRLGLRN*INNKATIYQAFTMC**V*NILFPLF LSAIHEIAIIILISYIWKQGQRSQVK*SNPDLE SWRLVETD*KSRFI*IKIYALNHHFC*SESVQP NSII*PKVLY*TVFWYDCEMSRPSSHRHTE*EIL AYIAVTIMLSIELAFIMFLCSFINSQ*CDYIFT LLKQIDWPHN*GNE*RWKSKCLAFLKPWVSVYA Q*NINNITERGCLYGSNGQ*QHWRDQVGTGCDHI LIYVLDDSIYDCSQVNFDAVNS*P*HLLLGTIL PSAPLLHEKTML*CFFFPGLSLADQQNLSSHFV LILKYQWWLSKFGRSDIKKEFQRFLYLFKMYFIV ALHTLNNFVYVNCQYYHVIK*IYF*NKVNVWLL GNFLFYHELLMKINLNTIAFKHLMLCKPYPLVY LKESILTMCFL*ELKHLGVCWTSMGLSCQYPGHI FELHLQLHLIGLRWALNKLG