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Sequences

Sequence APC.dAug05

GGCGGAAAAGCGGGCTGTCTCGGAAACTCAGAGCCGGGTTCCTCCCGGGTTTCTGCCGGGTTTCTCCCTGCGGCTCCTGGGTTGTTGAGACTCTTGTGAA GATGGCTTGCGCTGCCGCGCGGTCCCCGGCCGACCAGGACAGGTTTATTTGTATCTATCCTGCTTATTTAAATAATAAGAAGACCATCGCAGAGGGAAGG CGAATCCCCATAAGTAAGTGTTTATGTTTTTAACTGTTCAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAA CGTATTTCTTGAGGTTAAGAATGTCTGAGGGGTCAGGCGGTGCTGGCAAGATGGCTGCACTTGAGAAGATGACGTTTCCCAAGAAGATGACATTTCCAGA GAAACCAAGCCACAAAAAGTACAGGGCCGCCCTGAAGAAGGAGAAACGAAAGAAACGTCGGCAGGAACTTGCTCGACTGAGAGACTCAGGACTCTCACAG GAGGAGGAAGAGGACACTTTTATTGAAGAACAACAACTAGAAGAAGAGAAGCTATTGGAAAGAGAGAGGGAAAGATTACATGAGGAGTGGTTGCTGAGGG AGCAGAAGGCACAAGAAGAATTCAGAATAAAGAAGGAAAAGGAAGAGGCGGCTAAAAAATGGCTAGAAGAACAAGAGAGAAAGTTAAAGGAACAATGGAA AGAACAGCAGAGGAAAGAGAGAGAAGAGGAGGAGCAGAAACAACAGGAGAAGAAAGAAAAAGAGGAAGCTGTGCAGAAGATGCTGGATCAGGCTGAAAAT GATTTAGAAAATAGTACCACATGGCAAAACCCAGAACCACCCGTGGATTTCAGAGTAATGGAGAAGGATCGAGCTAATTGTCCCTTCTACAGTAAAACAG GAGCTTGCAGATTTGGAGACAGATGTTCACGTAAACATAATTTCCCAACATCTAGTCCTACCCTTCTTATTAAGAGCATGTTTACAACGTTTGGAATGGA GCAGTGCAGGAGGGATGACTATGACCCTGACGCAAGCCTGGAGTACAGCGAGGAAGAAACCTACCAACAGTTCCTAGATTTCTATGAGGATGTGTTGCCC GAGTTCAAGAACGTGGGGAAAGTGATTCAGTTCAAGGTCAGCTGCAATTTGGAACCTCACCTGAGGGGCAATGTATATGTTCAGTACCAGTCGGAAGAAG AATGCCAAGCAGCCCTTTCTCTGTTTAACGGACGATGGTATGCAGGACGACAGCTGCAATGTGAATTCTGCCCAGTGACCCGGTGGAAAATGGCGATTTG TGGTTTATTTGAAATACAACAATGTCCAAGAGGAAAACACTGCAACTTTCTTCATGTGTTCAGAAATCCCAACAATGAATTCTGGGAAGCTAATAGAGAC ATCTACTTGTCTTCAGATCAGACTGGCTCCTCCTTTGGCAAGAACTCCGAGAGGAGGGAGAAGATGGGCCACCACGACCACTACTACAGCAGGCAGCGGG GAAGGAGAAACCCTAGTCCAGACCACACCTACAAAAGAAATGGGGAATCCGAGAGAAAAAAGAGTAGTCATAGGGGGAAGAAATCTCACAAACGCACATC AAAGAGTCGGGAGAGGCACAATTCACCAAGCAGAGGAAGAAATAGGCACCGCAGCTGGGACCAGGGCCGCCGGAGCCAGAGCCGCAGGAGCCACCGCAGC CGGAGCCAAAGTTCCTCTAGGTGCCGAAGTCGTGGGAGGAGGAAGTCGGGTAATAGAGACAGAACTGTTCAGAGTCCCCAATCCAAATAAACTAGTTTTG TTCTT

APC.F16 (forward primer)
APC.R14 (reverse primer)
Single exon skipping

Frame 0

GGKAGCLGNSEPGSSRVSAGFLPAAPGLLRLL* RWLALPRGPRPTRTGLFVSILLI*IIRRPSQREG ESP*VSVYVFNCSGC*KSYSYRDSRCMFSSWT* RIS*G*ECLRGQAVLARWLHLRR*RFPRR*HFQ RNQATKSTGPP*RRRNERNVGRNLLD*ETQDSHR RRKRTLLLKNNN*KKRSYWKERGKDYMRSGC*G SRRHKKNSE*RRKRKRRLKNG*KNKRES*RNNG KNSRGKREKRRSRNNRRRKKKRKLCRRCWIRLKM I*KIVPHGKTQNHPWISE*WRRIELIVPSTVKQ ELADLETDVHVNIISQHLVLPFLLRACLQRLEW SSAGGMTMTLTQAWSTARKKPTNSS*ISMRMCCP SSRTWGK*FSSRSAAIWNLT*GAMYMFSTSRKK NAKQPFLCLTDDGMQDDSCNVNSAQ*PGGKWRF VVYLKYNNVQEENTATFFMCSEIPTMNSGKLIET STCLQIRLAPPLARTPRGGRRWATTTTTTAGSG EGETLVQTTPTKEMGNPREKRVVIGGRNLTNAH QRVGRGTIHQAEEEIGTAAGTRAAGARAAGATAA GAKVPLGAEVVGGGSRVIETELFRVPNPNKLVL F

Frame 1

AEKRAVSETQSRVPPGFLPGFSLRLLGC*DSCE DGLRCRAVPGRPGQVYLYLSCLFK**EDHRRGK ANPHK*VFMFLTVQAVENPTATEIQDVCSAVGLN VFLEVKNV*GVRRCWQDGCT*EDDVSQEDDISR ETKPQKVQGRPEEGETKETSAGTCSTERLRTLT GGGRGHFY*RTTTRRREAIGKREGKIT*GVVAEG AEGTRRIQNKEGKGRGG*KMARRTREKVKGTME RTAEERERRGGAETTGEERKRGSCAEDAGSG*K *FRK*YHMAKPRTTRGFQSNGEGSS*LSLLQ*NR SLQIWRQMFT*T*FPNI*SYPSY*EHVYNVWNG AVQEG*L*P*RKPGVQRGRNLPTVPRFL*GCVA RVQERGESDSVQGQLQFGTSPEGQCICSVPVGRR MPSSPFSV*RTMVCRTTAAM*ILPSDPVENGDL WFI*NTTMSKRKTLQLSSCVQKSQQ*ILGS**R HLLVFRSDWLLLWQELREEGEDGPPRPLLQQAAG KEKP*SRPHLQKKWGIREKKE*S*GEEISQTHI KESGEAQFTKQRKK*APQLGPGPPEPEPQEPPQ PEPKFL*VPKSWEEEVG**RQNCSESPIQIN*FC S

Frame 2

RKSGLSRKLRAGFLPGFCRVSPCGSWVVETLVK MACAAARSPADQDRFICIYPAYLNNKKTIAEGR RIPISKCLCF*LFRLLKILQLQRFKMYVQQLDL TYFLRLRMSEGSGGAGKMAALEKMTFPKKMTFPE KPSHKKYRAALKKEKRKKRRQELARLRDSGLSQ EEEEDTFIEEQQLEEEKLLERERERLHEEWLLR EQKAQEEFRIKKEKEEAAKKWLEEQERKLKEQWK EQQRKEREEEEQKQQEKKEKEEAVQKMLDQAEN DLENSTTWQNPEPPVDFRVMEKDRANCPFYSKT GACRFGDRCSRKHNFPTSSPTLLIKSMFTTFGME QCRRDDYDPDASLEYSEEETYQQFLDFYEDVLP EFKNVGKVIQFKVSCNLEPHLRGNVYVQYQSEE ECQAALSLFNGRWYAGRQLQCEFCPVTRWKMAIC GLFEIQQCPRGKHCNFLHVFRNPNNEFWEANRD IYLSSDQTGSSFGKNSERREKMGHHDHYYSRQR GRRNPSPDHTYKRNGESERKKSSHRGKKSHKRTS KSRERHNSPSRGRNRHRSWDQGRRSQSRRSHRS RSQSSSRCRSRGRRKSGNRDRTVQSPQSK*TSF VL

Sequence APC.qAug05

GCCACGCAGAAAAAGGAGGGGTTGAAAAGGGAGTACCCATCTGATATCTAGGCAGCATGAATTGGCCTTTGGTTCCCTGCCACCCGACCCTTTTCTCCTG CCTCTTACTGCTCTAGTGATACCACTTATGCTATTGTCTATGGCAGGTTTATTTGTATCTATCCTGCTTATTTAAATAATAAGAAGACCATCGCAGAGGG AAGGCGAATCCCCATAAGTAAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTTCTTGAGAAAAAT AAAATGTACTCTAGAGAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAGCCTCTGCCTTGTACAGTTCC CATCACGTTAAGAATGTCTGAGGGGTCAGGCGGTGCTGGCAAGATGGCTGCACTTGAGAAGATGACGTTTCCCAAGAAGATGACATTTCCAGAGAAACCA AGCCACAAAAAGTACAGGGCCGCCCTGAAGAAGGAGAAACGAAAGAAACGTCGGCAGGAACTTGCTCGACTGAGAGACTCAGGACTCTCACAGGAGGAGG AAGAGGACACTTTTATTGAAGAACAACAACTAGAAGAAGAGAAGCTATTGGAAAGAGAGAGGGAAAGATTACATGAGGAGTGGTTGCTGAGGGAGCAGAA GGCACAAGAAGAATTCAGAATAAAGAAGGAAAAGGAAGAGGCGGCTAAAAAATGGCTAGAAGAACAAGAGAGAAAGTTAAAGGAACAATGGAAAGAACAG CAGAGGAAAGAGAGAGAAGAGGAGGAGCAGAAACAACAGGAGAAGAAAGAAAAAGAGGAAGCTGTGCAGAAGATGCTGGATCAGGCTGAAAATGATTTAG AAAATAGTACCACATGGCAAAACCCAGAACCACCCGTGGATTTCAGAGTAATGGAGAAGGATCGAGCTAATTGTCCCTTCTACAGTAAAACAGGAGCTTG CAGATTTGGAGACAGATGTTCACGTAAACATAATTTCCCAACATCTAGTCCTACCCTTCTTATTAAGAGCATGTTTACAACGTTTGGAATGGAGCAGTGC AGGAGGGATGACTATGACCCTGACGCAAGCCTGGAGTACAGCGAGGAAGAAACCTACCAACAGTTCCTAGATTTCTATGAGGATGTGTTGCCCGAGTTCA AGAACGTGGGGAAAGTGATTCAGTTCAAGGTCAGCTGCAATTTGGAACCTCACCTGAGGGGCAATGTATATGTTCAGTACCAGTCGGAAGAAGAATGCCA AGCAGCCCTTTCTCTGTTTAACGGACGATGGTATGCAGGACGACAGCTGCAATGTGAATTCTGCCCAGTGACCCGGTGGAAAATGGCGATTTGTGGTTTA TTTGAAATACAACAATGTCCAAGAGGAAAACACTGCAACTTTCTTCATGTGTTCAGAAATCCCAACAATGAATTCTGGGAAGCTAATAGAGACATCTACT TGTCTTCAGATCAGACTGGCTCCTCCTTTGGCAAGAACTCCGAGAGGAGGGAGAAGATGGGCCACCACGACCACTACTACAGCAGGCAGCGGGGAAGGAG AAACCCTAGTCCAGACCACACCTACAAAAGAAATGGGGAATCCGAGAGAAAAAAGAGTAGTCATAGGGGGAAGAAATCTCACAAACGCACATCAAAGAGT CGGGAGAGGCACAATTCACCAAGCAGAGGAAGAAATAGGCACCGCAGCTGGGACCAGGGCCGCCGGAGCCAGAGCCGCAGGAGCCACCGCAGCCGGAGCC AAAGTTCCTCTAGGTGCCGAAGTCGTGGGAGGAGGAAGTCGGGTAATAGAGACAGAACTGTTCAGAGTCCCCAATCCAAATAAACTAGTTTTGTTCTT

APC.F16 (forward primer)
APC.R14 (reverse primer)
Single exon skipping

Frame 0

ATQKKEGLKREYPSDI*AA*IGLWFPATRPFSP ASYCSSDTTYAIVYGRFICIYPAYLNNKKTIAEG RRIPISKAVENPTATEIQDVCSAVGLNVFLEKN KMYSREWNRDVQYRGRVRVQLKQEDGSLCLVQF PSR*ECLRGQAVLARWLHLRR*RFPRR*HFQRNQ ATKSTGPP*RRRNERNVGRNLLD*ETQDSHRRR KRTLLLKNNN*KKRSYWKERGKDYMRSGC*GSR RHKKNSE*RRKRKRRLKNG*KNKRES*RNNGKNS RGKREKRRSRNNRRRKKKRKLCRRCWIRLKMI* KIVPHGKTQNHPWISE*WRRIELIVPSTVKQEL ADLETDVHVNIISQHLVLPFLLRACLQRLEWSSA GGMTMTLTQAWSTARKKPTNSS*ISMRMCCPSS RTWGK*FSSRSAAIWNLT*GAMYMFSTSRKKNA KQPFLCLTDDGMQDDSCNVNSAQ*PGGKWRFVVY LKYNNVQEENTATFFMCSEIPTMNSGKLIETST CLQIRLAPPLARTPRGGRRWATTTTTTAGSGEG ETLVQTTPTKEMGNPREKRVVIGGRNLTNAHQRV GRGTIHQAEEEIGTAAGTRAAGARAAGATAAGA KVPLGAEVVGGGSRVIETELFRVPNPNKLVLF

Frame 1

PRRKRRG*KGSTHLISRQHELAFGSLPPDPFLL PLTALVIPLMLLSMAGLFVSILLI*IIRRPSQR EGESP*VRLLKILQLQRFKMYVQQLDLTYFLRKI KCTLENGIVMSNTEAESGSSSNRKMGASALYSS HHVKNV*GVRRCWQDGCT*EDDVSQEDDISRET KPQKVQGRPEEGETKETSAGTCSTERLRTLTGGG RGHFY*RTTTRRREAIGKREGKIT*GVVAEGAE GTRRIQNKEGKGRGG*KMARRTREKVKGTMERT AEERERRGGAETTGEERKRGSCAEDAGSG*K*FR K*YHMAKPRTTRGFQSNGEGSS*LSLLQ*NRSL QIWRQMFT*T*FPNI*SYPSY*EHVYNVWNGAV QEG*L*P*RKPGVQRGRNLPTVPRFL*GCVARVQ ERGESDSVQGQLQFGTSPEGQCICSVPVGRRMP SSPFSV*RTMVCRTTAAM*ILPSDPVENGDLWF I*NTTMSKRKTLQLSSCVQKSQQ*ILGS**RHLL VFRSDWLLLWQELREEGEDGPPRPLLQQAAGKE KP*SRPHLQKKWGIREKKE*S*GEEISQTHIKE SGEAQFTKQRKK*APQLGPGPPEPEPQEPPQPEP KFL*VPKSWEEEVG**RQNCSESPIQIN*FCS

Frame 2

HAEKGGVEKGVPI*YLGSMNWPLVPCHPTLFSC LLLL**YHLCYCLWQVYLYLSCLFK**EDHRRG KANPHK*GC*KSYSYRDSRCMFSSWT*RIS*EK *NVL*RMES*CPIQRQSPGPAQTGRWEPLPCTVP ITLRMSEGSGGAGKMAALEKMTFPKKMTFPEKP SHKKYRAALKKEKRKKRRQELARLRDSGLSQEE EEDTFIEEQQLEEEKLLERERERLHEEWLLREQK AQEEFRIKKEKEEAAKKWLEEQERKLKEQWKEQ QRKEREEEEQKQQEKKEKEEAVQKMLDQAENDL ENSTTWQNPEPPVDFRVMEKDRANCPFYSKTGAC RFGDRCSRKHNFPTSSPTLLIKSMFTTFGMEQC RRDDYDPDASLEYSEEETYQQFLDFYEDVLPEF KNVGKVIQFKVSCNLEPHLRGNVYVQYQSEEECQ AALSLFNGRWYAGRQLQCEFCPVTRWKMAICGL FEIQQCPRGKHCNFLHVFRNPNNEFWEANRDIY LSSDQTGSSFGKNSERREKMGHHDHYYSRQRGRR NPSPDHTYKRNGESERKKSSHRGKKSHKRTSKS RERHNSPSRGRNRHRSWDQGRRSQSRRSHRSRS QSSSRCRSRGRRKSGNRDRTVQSPQSK*TSFVL