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Sequences

Sequence FN1.jAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCA CACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGA AATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACA AGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCT GGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAG CTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCC ACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACA TCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAAC CGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGGACAAGAAGCTCTCTCTCAGACAACC ATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTT CTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGT TGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAG TGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACA ATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAA GTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACA TGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATT CTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTA AATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTT CTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATG AGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCAT ATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCC GCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATC TTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTAT AATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAA GTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTA ATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTT CTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACC ATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAA AAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACT TTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGG GATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGA GACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCT GAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCA AAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGC AAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCAT GTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAG CTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAG CCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNHYSCTN*PEVHSGH THKPERPVDTTQCSAHWISSAGDPQGEDRTNER NQPCS*QLIRGCIRTYGGHQI*SECLCS*GHFD KQTSSGSCHHSGECQPTKKGSCDRCY*DHHHH*L ENQD*DDHWLPS*CRSSQWPDSNPENHQARCQK LHHHRFTTRH*LQDLPVHLE*QCSELPCGHRRL HCH*CTIQPAFPGHHTQFLAGIMAAATCQDYRLH HQV*EAWVSSQRSGPSAPPWCHRGYYYWPGTGN RIYNLCHCPEE*SEERAPDWKEKDRTRSSLSDN HLMGPIPGHF*VHHFMSSCWH**RTLTVQGSWNF YQCHSDRPHQRCHLQRHSGGTERPAEA*GSGRG CYRGQLCQRRLEPTYG*LVL*PLHSFPLCRWR* VGTNV*IRL*TVVPVLRLWKWSFQM*FI*MVP*Q WCELQDWREVGPSGRKWPDDELHMSWERKRRIQ V*PS*GNVL**WEDIPRRRTVAEGISRCHLLLH MLWRPAGLAL*QLPQTWG*TQSRRHYWPVLQPVF SEIPSENKH*C*LPN*VLHAFRCTG*QRRFPRV NHLSNPEEQACLSAKIHLNWSDVSRPSLEFFFL S*ALCSGGSSPASAQLTASPSITLGVS*GFSHK* GLHIACSASKYSIPLSILNEVILRFGLGSIGKH MQPTKMQMF*NDMTKILSRKVTQTLLLSLKCLA RNTVGTSMILLL*YFKYPQYSLFPNDPSNCLEIS FSYLLFINFSQYFYTEKIVLKTLSMQLIRGIWY NYGG*LFFILYVPKLYYCGKTTVLIKDLHSTT* SSSI*YKTPSGEIIPVNILFQFSKHLKIYDVQV* LLISVQDFLNQLLQKLIGFCHFISSLMEIALHF LL**I*VDPNIY*NC*FTVQKYNRNNL*LLFSN HCNSSLLPSTFPSLNKPLFKEIACKGNKND*DIK SI*IV*YGGVLS*GNPMV**PPSNMSYFGSADT FGMA**TPS*TVCLLLICKGHFFFNLTLRKQTL GLNLTFRPGAVAHTSNPSTLGG*GRWIT*GQEFE TSLANMVKPCLY*KYKCYQAWQWASLVPATQKA EVGEWLEPRRRRLQ*AEIVPLNSSMGDRARLHP KKHK*INVTFRIVNSGNGNNY**KISSS**TRHA KHFLNILMYNERRYFNKHLAKNIKTKYRKILPH VHLKKKNTNSHLN*FTVYKML*HLAISFRGGFR A*FYRA*SSDEVSLAKYRPSPWTSIYY*SLSFIS PIVTKRAALYVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQV TPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKE INLAPDSSSVVVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITIS WRTKTETITGFQVDAVPANGQTPIQRTIKPDVRS YTITGLQPGTDYKIYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLLVSWQPPRARITGY IIKYEKPGSPPREVVPRPRPGVTEATITGLEPGT EYTIYVIALKNNQKSEPLIGRKKTGQEALSQTT ISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGT STSATLTGLTRGATYNVIVEALKDQQRHKVREEV VTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDE WERMSESGFKLLCQCLGFGSGHFRCDSSRWCHD NGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFK CDPHEATCYDDGKTYHVGEQWQKEYLGAICSCT CFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQY SQRYHQRTNTNVNCPIECFMPLDVQADREDSRE* IIFPIQRNKHVSLPRSI*TGVMLADPA*SSSFF LKPFALEEVLQLQLNSQLLQASPWEFPEGFLIN EGCTLPVLLRSIQYRSVF*MK*F*DLVWDQ*ESI CSQPRCKCFEMI*PKF*VGKSPKHFCFHLSVWP AIL*EQA*SCYCDILNIHSTHFFQMILVIA*KY LSLTCYLSIFPSIFIRKKLY*KHLVCS**EEFGI IMVGDYFLYCMCQSFTTVERQLF**KIYIPQLE VHLFDIRHLRGK*FL*IFFFNSANI*KSMMCKS NC*FQYKIF*ISCYKN*LVFVTSSLH*WR*LYTF CFNRFKWTPIFIKIASLPFRSIIEIIFSCSFLT IVILPFFPPPFLH*INLCSKRLPAREIKMTKIL KVFE*YNMEEFYLRETPWYDNPHLTCLTLGQLTL LAWLDKLPAKQFVYS*SVKVISFSI*H*GNKLW D*I*HSGQAQWLTPLIPALWEAEAGGSPEVRSL RPAWPTW*NLVSIKNTNVTRHGSGRLWSRLLRRL R*ENGLNPEGGGCSELRLCH*TPAWGTEQDSIP KNINK*M*HSE**IVEMEIIISKKLVVHNKQDM QSTF*IY*CTMKEGISINTWQRILKLNTERYFLM FI*KKKILTPI*IDLQFIKCFDI*LSHLGEVLE LNFTGHEAQMRFP*RNTDPAPGLLFITDL*ALS AQ*SPRELLCMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PLFLHQLT*SSLRS HPQA*APSGHHPMFSSLDIECG*PPRRRPDQ*K KSTLLLTAHPWLYQDLWWPPNMK*VSMLLRTL*Q ADQLRELSPLWRMSAHQEGLV*QMLLRPPSPLA GEPRLRRSLASKLMPFQPMARLQSREPSSQMSE ATPSQVYNQALTTRSTCTP*MTMLGAPLWSSTPP LPLMHHPTCVSWPPHPIPCWYHGSRHVPGLPAT SSSMRSLGLLPEKWSLGPALVSQRLLLLAWNRE PNIQFMSLP*RIIRRASP*LEGKRQDKKLSLRQP SHGPHSRTLLSTSFHVILLALMKNPYSSGFLEL LPVPL*QASPEVPPTTS*WRH*KTSRGIRFGKR LLPWATLSTKA*TNLRMTRALTPTQFPIMPLEMS GNECLNQALNCCASA*ALEVVISDVIHLDGAMT MV*TTRLERSGTVREKMAR**AAHVLGTEKENS SVTLMRQRVMMMGRHTT*ENSGRRNISVPFAPAH ALEASGAGAVTTAADLGVNPVPKALLASPTTSI LRDTIREQTLMLIAQLSASCL*MYRLTEKIPES KSSFQSRGTSMSLCQDPSKLE*C*QTQLRVLLSF LSPLLWRKFSSFSSTHSFSKHHPGSFLRVFS*M RAAHCLFCFEVFNTAQYFK*SDSKIWFGINRKA YAANQDANVLK*YDQNFK*ESHPNTSAFT*VSGP QYCRNKHDLVTVIF*ISTVLTFSK*S**LPRNI FLLPVIYQFFPVFLYGKNCIENT*YAVDKRNLV *LWWVIIFYTVCAKALLLWKDNCFNKRFTFHNLK FIYLI*DTFGGNNSCEYSFSIQQTFENL*CASL IVDFSTRFSKSVATKTDWFLSLHLFTNGDSFTL SALIDLSGPQYLLKLLVYRSEV**K*SLVALF*P L*FFPSSLHLSFIE*TSVQRDCLQGK*K*LRY* KYLNSIIWRSFILGKPHGMITPI*HVLLWVS*H FWHGLINSQLNSLFTPDL*RSFLFQFNIEETNFG IKFDIQARRSGSHL*SQHFGRLRQVDHLRSGV* DQPGQHGETLSLLKIQMLPGMAVGVFGPGYSEG *GRRMA*TQKAEVAVS*DCAIELQHGGQSKTPSQ KT*INKCDIQNSK*WKWK*LLVKN**FIINKTC KALFKYTNVQ*KKVFQ*TPGKEY*N*IQKDTSS CSFEKKKY*LPFELIYSL*NALTFSYLI*GRF*S LILQGMKLR*GFLSEIQTQPLDFYLLLIFKLYQ PNSHQESCSVCFLKITESTK*R*Y*TTV

Sequence FN1.iAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCA CACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGA AATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACA AGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCT GGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAG CTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCC ACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACA TCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAAC CGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGTTCAAAAGACCCCTTTCGTCACCCAC CCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGC GGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATG GGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGT GCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCG TGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACG AATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTG AACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACC CTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGG AGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGA TACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCT TTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCC CTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGC ACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCC AACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACT GTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTT ACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGG TGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCT ATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGA TTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTA ATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAAT TCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTT GAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCAT GGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAAT TTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCC TGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGG AGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACAT AAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACT TTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTT GAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTT TACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAG TCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNHYSCTN*PEVHSGH THKPERPVDTTQCSAHWISSAGDPQGEDRTNER NQPCS*QLIRGCIRTYGGHQI*SECLCS*GHFD KQTSSGSCHHSGECQPTKKGSCDRCY*DHHHH*L ENQD*DDHWLPS*CRSSQWPDSNPENHQARCQK LHHHRFTTRH*LQDLPVHLE*QCSELPCGHRRL HCH*CTIQPAFPGHHTQFLAGIMAAATCQDYRLH HQV*EAWVSSQRSGPSAPPWCHRGYYYWPGTGN RIYNLCHCPEE*SEERAPDWKEKDSSKDPFRHP PWV*HWKWYSASWHFWSATQCWATNDL*GTWF*A DHTAHNGHPHKA*AKTIPAECRTRSSLSDNHLM GPIPGHF*VHHFMSSCWH**RTLTVQGSWNFYQ CHSDRPHQRCHLQRHSGGTERPAEA*GSGRGCYR GQLCQRRLEPTYG*LVL*PLHSFPLCRWR*VGT NV*IRL*TVVPVLRLWKWSFQM*FI*MVP*QWC ELQDWREVGPSGRKWPDDELHMSWERKRRIQV*P S*GNVL**WEDIPRRRTVAEGISRCHLLLHMLW RPAGLAL*QLPQTWG*TQSRRHYWPVLQPVFSE IPSENKH*C*LPN*VLHAFRCTG*QRRFPRVNHL SNPEEQACLSAKIHLNWSDVSRPSLEFFFLS*A LCSGGSSPASAQLTASPSITLGVS*GFSHK*GL HIACSASKYSIPLSILNEVILRFGLGSIGKHMQP TKMQMF*NDMTKILSRKVTQTLLLSLKCLARNT VGTSMILLL*YFKYPQYSLFPNDPSNCLEISFS YLLFINFSQYFYTEKIVLKTLSMQLIRGIWYNYG G*LFFILYVPKLYYCGKTTVLIKDLHSTT*SSS I*YKTPSGEIIPVNILFQFSKHLKIYDVQV*LL ISVQDFLNQLLQKLIGFCHFISSLMEIALHFLL* *I*VDPNIY*NC*FTVQKYNRNNL*LLFSNHCN SSLLPSTFPSLNKPLFKEIACKGNKND*DIKSI *IV*YGGVLS*GNPMV**PPSNMSYFGSADTFGM A**TPS*TVCLLLICKGHFFFNLTLRKQTLGLN LTFRPGAVAHTSNPSTLGG*GRWIT*GQEFETS LANMVKPCLY*KYKCYQAWQWASLVPATQKAEVG EWLEPRRRRLQ*AEIVPLNSSMGDRARLHPKKH K*INVTFRIVNSGNGNNY**KISSS**TRHAKH FLNILMYNERRYFNKHLAKNIKTKYRKILPHVHL KKKNTNSHLN*FTVYKML*HLAISFRGGFRA*F YRA*SSDEVSLAKYRPSPWTSIYY*SLSFISPI VTKRAALYVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQV TPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKE INLAPDSSSVVVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITIS WRTKTETITGFQVDAVPANGQTPIQRTIKPDVRS YTITGLQPGTDYKIYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLLVSWQPPRARITGY IIKYEKPGSPPREVVPRPRPGVTEATITGLEPGT EYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTH PGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFR RTTPPTTATPIRHRPRPYPPNVGQEALSQTTISW APFQDTSEYIISCHPVGTDEEPLQFRVPGTSTS ATLTGLTRGATYNVIVEALKDQQRHKVREEVVT VGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWER MSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGV NYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCD PHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFG GQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQR YHQRTNTNVNCPIECFMPLDVQADREDSRE*II FPIQRNKHVSLPRSI*TGVMLADPA*SSSFFLKP FALEEVLQLQLNSQLLQASPWEFPEGFLINEGC TLPVLLRSIQYRSVF*MK*F*DLVWDQ*ESICS QPRCKCFEMI*PKF*VGKSPKHFCFHLSVWPAIL *EQA*SCYCDILNIHSTHFFQMILVIA*KYLSL TCYLSIFPSIFIRKKLY*KHLVCS**EEFGIIM VGDYFLYCMCQSFTTVERQLF**KIYIPQLEVHL FDIRHLRGK*FL*IFFFNSANI*KSMMCKSNC* FQYKIF*ISCYKN*LVFVTSSLH*WR*LYTFCF NRFKWTPIFIKIASLPFRSIIEIIFSCSFLTIVI LPFFPPPFLH*INLCSKRLPAREIKMTKILKVF E*YNMEEFYLRETPWYDNPHLTCLTLGQLTLLA WLDKLPAKQFVYS*SVKVISFSI*H*GNKLWD*I *HSGQAQWLTPLIPALWEAEAGGSPEVRSLRPA WPTW*NLVSIKNTNVTRHGSGRLWSRLLRRLR* ENGLNPEGGGCSELRLCH*TPAWGTEQDSIPKNI NK*M*HSE**IVEMEIIISKKLVVHNKQDMQST F*IY*CTMKEGISINTWQRILKLNTERYFLMFI *KKKILTPI*IDLQFIKCFDI*LSHLGEVLELNF TGHEAQMRFP*RNTDPAPGLLFITDL*ALSAQ* SPRELLCMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PLFLHQLT*SSLRS HPQA*APSGHHPMFSSLDIECG*PPRRRPDQ*K KSTLLLTAHPWLYQDLWWPPNMK*VSMLLRTL*Q ADQLRELSPLWRMSAHQEGLV*QMLLRPPSPLA GEPRLRRSLASKLMPFQPMARLQSREPSSQMSE ATPSQVYNQALTTRSTCTP*MTMLGAPLWSSTPP LPLMHHPTCVSWPPHPIPCWYHGSRHVPGLPAT SSSMRSLGLLPEKWSLGPALVSQRLLLLAWNRE PNIQFMSLP*RIIRRASP*LEGKRQFKRPLSSPT LGMTLEMVFSFLALLVSNPVLGNK*SLRNMVLG GPHRPQRPPP*GIGQDHTRRM*DKKLSLRQPSH GPHSRTLLSTSFHVILLALMKNPYSSGFLELLPV PL*QASPEVPPTTS*WRH*KTSRGIRFGKRLLP WATLSTKA*TNLRMTRALTPTQFPIMPLEMSGN ECLNQALNCCASA*ALEVVISDVIHLDGAMTMV* TTRLERSGTVREKMAR**AAHVLGTEKENSSVT LMRQRVMMMGRHTT*ENSGRRNISVPFAPAHAL EASGAGAVTTAADLGVNPVPKALLASPTTSILRD TIREQTLMLIAQLSASCL*MYRLTEKIPESKSS FQSRGTSMSLCQDPSKLE*C*QTQLRVLLSFLS PLLWRKFSSFSSTHSFSKHHPGSFLRVFS*MRAA HCLFCFEVFNTAQYFK*SDSKIWFGINRKAYAA NQDANVLK*YDQNFK*ESHPNTSAFT*VSGPQY CRNKHDLVTVIF*ISTVLTFSK*S**LPRNIFLL PVIYQFFPVFLYGKNCIENT*YAVDKRNLV*LW WVIIFYTVCAKALLLWKDNCFNKRFTFHNLKFI YLI*DTFGGNNSCEYSFSIQQTFENL*CASLIVD FSTRFSKSVATKTDWFLSLHLFTNGDSFTLSAL IDLSGPQYLLKLLVYRSEV**K*SLVALF*PL* FFPSSLHLSFIE*TSVQRDCLQGK*K*LRY*KYL NSIIWRSFILGKPHGMITPI*HVLLWVS*HFWH GLINSQLNSLFTPDL*RSFLFQFNIEETNFGIK FDIQARRSGSHL*SQHFGRLRQVDHLRSGV*DQP GQHGETLSLLKIQMLPGMAVGVFGPGYSEG*GR RMA*TQKAEVAVS*DCAIELQHGGQSKTPSQKT *INKCDIQNSK*WKWK*LLVKN**FIINKTCKAL FKYTNVQ*KKVFQ*TPGKEY*N*IQKDTSSCSF EKKKY*LPFELIYSL*NALTFSYLI*GRF*SLI LQGMKLR*GFLSEIQTQPLDFYLLLIFKLYQPNS HQESCSVCFLKITESTK*R*Y*TTV

Sequence FN1.hAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGAGGTGCCCCAACTCACTGACCTAAGCTTTGTTGA TATAACCGATTCAAGCATCGGCCTGAGGTGGACCCCGCTAAACTCTTCCACCATTATTGGGTACCGCATCACAGTAGTTGCGGCAGGAGAAGGTATCCCT ATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTCACAGGGCTGGAGCCGGGCATTGACTATGATATCAGCGTTATCACTCTCATTAATG GCGGCGAGAGTGCCCCTACTACACTGACACAACAAACGGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAACATTGGTCCAGACACCATGCGTGTCAC CTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGATGTTGCAGAGTTGTCAATTTCTCCTTCA GACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAACAACATGAGAGCACACCTCTTAGAGGAA GACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCACTGGATTGCTCCTCGAGCCACCATCAC TGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGGAATTCCATCACCCTCACCAACCTCACT CCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAACAATCAACAGTTTCTGATGTTCCGAGGG ACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATATTACAGGATCACTTACGGAGAGACAGG AGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCTGGAGTTGATTATACCATCACTGTGTAT GCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACAAACCATCCCAGATGCAAGTGACCGATG TTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCACTCCCAAAAATGGACCAGGACCAACAAA AACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTTAGTGTCTATGCTCAGAATCCAAGCGGA GAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGA CACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATC CGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACC ACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTG GCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGG CACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTG CGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTC CTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCT GAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACT TCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCA CCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGA AGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTT AAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGA AGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTA TGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGC TGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACA CTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAA GCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTT CTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTC GAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTT TGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCT TGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTT TTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATA CTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTT CGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCT AAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCC AATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCA CCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGG AGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGC TAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCG CAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACC TTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGA AGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACAT TCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTA CAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAAC TCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAG ATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCT GTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPRGAPTH*PKLC* YNRFKHRPEVDPAKLFHHYWVPHHSSCGRRRYP YF*RFCGLLSRILHSHRAGAGH*L*YQRYHSH*W RRECPYYTDTTNGCSSSH*PAIHQHWSRHHACH LGSTPIH*FNQLPGALLTCEK*GRCCRVVNFSF RQCSGLNKSPAWYRICSECLQCLRTT*EHTS*RK TENRS*FPNWH*LF*YYCQLFYCALDCSSSHHH WLQDPPSSRALQWETSRRSGAPLSEFHHPHQPH SRHRVCGQHRCS*WQRGKSLIDWPTINSF*CSEG PGSCCCDPHQPTDQLGCSCCHSEILQDHLRRDR RK*PCPGVHCAWEQVYSYHQRP*TWS*LYHHCV CCHWPWRQPRKQQANFH*LPNRN*QTIPDASDRC SGQQH*CQVAAFKFPCYWLQSNHHSQKWTRTNK N*NCRSRSNRNDY*RLAAHSGVCG*CLCSESKR RESASGSDCSNHYSCTN*PEVHSGHTHKPERPVD TTQCSAHWISSAGDPQGEDRTNERNQPCS*QLI RGCIRTYGGHQI*SECLCS*GHFDKQTSSGSCH HSGECQPTKKGSCDRCY*DHHHH*LENQD*DDHW LPS*CRSSQWPDSNPENHQARCQKLHHHRFTTR H*LQDLPVHLE*QCSELPCGHRRLHCH*CTIQP AFPGHHTQFLAGIMAAATCQDYRLHHQV*EAWVS SQRSGPSAPPWCHRGYYYWPGTGNRIYNLCHCP EE*SEERAPDWKEKDRTRSSLSDNHLMGPIPGH F*VHHFMSSCWH**RTLTVQGSWNFYQCHSDRPH QRCHLQRHSGGTERPAEA*GSGRGCYRGQLCQR RLEPTYG*LVL*PLHSFPLCRWR*VGTNV*IRL *TVVPVLRLWKWSFQM*FI*MVP*QWCELQDWRE VGPSGRKWPDDELHMSWERKRRIQV*PS*GNVL **WEDIPRRRTVAEGISRCHLLLHMLWRPAGLA L*QLPQTWG*TQSRRHYWPVLQPVFSEIPSENKH *C*LPN*VLHAFRCTG*QRRFPRVNHLSNPEEQ ACLSAKIHLNWSDVSRPSLEFFFLS*ALCSGGS SPASAQLTASPSITLGVS*GFSHK*GLHIACSAS KYSIPLSILNEVILRFGLGSIGKHMQPTKMQMF *NDMTKILSRKVTQTLLLSLKCLARNTVGTSMI LLL*YFKYPQYSLFPNDPSNCLEISFSYLLFINF SQYFYTEKIVLKTLSMQLIRGIWYNYGG*LFFI LYVPKLYYCGKTTVLIKDLHSTT*SSSI*YKTP SGEIIPVNILFQFSKHLKIYDVQV*LLISVQDFL NQLLQKLIGFCHFISSLMEIALHFLL**I*VDP NIY*NC*FTVQKYNRNNL*LLFSNHCNSSLLPS TFPSLNKPLFKEIACKGNKND*DIKSI*IV*YGG VLS*GNPMV**PPSNMSYFGSADTFGMA**TPS *TVCLLLICKGHFFFNLTLRKQTLGLNLTFRPG AVAHTSNPSTLGG*GRWIT*GQEFETSLANMVKP CLY*KYKCYQAWQWASLVPATQKAEVGEWLEPR RRRLQ*AEIVPLNSSMGDRARLHPKKHK*INVT FRIVNSGNGNNY**KISSS**TRHAKHFLNILMY NERRYFNKHLAKNIKTKYRKILPHVHLKKKNTN SHLN*FTVYKML*HLAISFRGGFRA*FYRA*SS DEVSLAKYRPSPWTSIYY*SLSFISPIVTKRAAL YVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVD ITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIP IFEDFVDSSVGYYTVTGLEPGIDYDISVITLIN GGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVT WAPPPSIDLTNFLVRYSPVKNEEDVAELSISPS DNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRG RQKTGLDSPTGIDFSDITANSFTVHWIAPRATIT GYRIRHHPEHFSGRPREDRVPHSRNSITLTNLT PGTEYVVSIVALNGREESPLLIGQQSTVSDVPR DLEVVAATPTSLLISWDAPAVTVRYYRITYGETG GNSPVQEFTVPGSKSTATISGLKPGVDYTITVY AVTGRGDSPASSKPISINYRTEIDKPSQMQVTD VQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTK TKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSG ESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQW TPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSS VVVSGLMVATKYEVSVYALKDTLTSRPAQGVVT TLENVSPPRRARVTDATETTITISWRTKTETIT GFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPG TDYKIYLYTLNDNARSSPVVIDASTAIDAPSNL RFLATTPNSLLVSWQPPRARITGYIIKYEKPGS PPREVVPRPRPGVTEATITGLEPGTEYTIYVIAL KNNQKSEPLIGRKKTGQEALSQTTISWAPFQDT SEYIISCHPVGTDEEPLQFRVPGTSTSATLTGL TRGATYNVIVEALKDQQRHKVREEVVTVGNSVNE GLNQPTDDSCFDPYTVSHYAVGDEWERMSESGF KLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGE KWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCY DDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWR CDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTN TNVNCPIECFMPLDVQADREDSRE*IIFPIQRNK HVSLPRSI*TGVMLADPA*SSSFFLKPFALEEV LQLQLNSQLLQASPWEFPEGFLINEGCTLPVLL RSIQYRSVF*MK*F*DLVWDQ*ESICSQPRCKCF EMI*PKF*VGKSPKHFCFHLSVWPAIL*EQA*S CYCDILNIHSTHFFQMILVIA*KYLSLTCYLSI FPSIFIRKKLY*KHLVCS**EEFGIIMVGDYFLY CMCQSFTTVERQLF**KIYIPQLEVHLFDIRHL RGK*FL*IFFFNSANI*KSMMCKSNC*FQYKIF *ISCYKN*LVFVTSSLH*WR*LYTFCFNRFKWTP IFIKIASLPFRSIIEIIFSCSFLTIVILPFFPP PFLH*INLCSKRLPAREIKMTKILKVFE*YNME EFYLRETPWYDNPHLTCLTLGQLTLLAWLDKLPA KQFVYS*SVKVISFSI*H*GNKLWD*I*HSGQA QWLTPLIPALWEAEAGGSPEVRSLRPAWPTW*N LVSIKNTNVTRHGSGRLWSRLLRRLR*ENGLNPE GGGCSELRLCH*TPAWGTEQDSIPKNINK*M*H SE**IVEMEIIISKKLVVHNKQDMQSTF*IY*C TMKEGISINTWQRILKLNTERYFLMFI*KKKILT PI*IDLQFIKCFDI*LSHLGEVLELNFTGHEAQ MRFP*RNTDPAPGLLFITDL*ALSAQ*SPRELL CMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQRCPNSLT*ALL I*PIQASA*GGPR*TLPPLLGTASQ*LRQEKVSL FLKILWTPQ*DTTQSQGWSRALTMISALSLSLM AARVPLLH*HNKRLFLLPLTCDSPTLVQTPCVS PGLHPHPLI*PTSWCVTHL*KMRKMLQSCQFLLQ TMQWS*QISCLVQNM**VSPVSTNNMRAHLLEE DRKQVLIPQLALTFLILLPTLLLCTGLLLEPPS LATGSAIIPSTSVGDLEKIGCPTLGIPSPSPTSL QAQSMWSASLLLMAERKVPY*LANNQQFLMFRG TWKLLLRPPPAY*SAGMLLLSQ*DITGSLTERQ EEIALSRSSLCLGASLQLPSAALNLELIIPSLCM LSLAVETAPQAASQFPLITEQKLTNHPRCK*PM FRTTALVSSGCLQVPLLLVTE*PPLPKMDQDQQ KLKLQVQIKQK*LLKACSPQWSMWLVSMLRIQAE RVSLWFRLQ*PLFLHQLT*SSLRSHPQA*APSG HHPMFSSLDIECG*PPRRRPDQ*KKSTLLLTAH PWLYQDLWWPPNMK*VSMLLRTL*QADQLRELSP LWRMSAHQEGLV*QMLLRPPSPLAGEPRLRRSL ASKLMPFQPMARLQSREPSSQMSEATPSQVYNQ ALTTRSTCTP*MTMLGAPLWSSTPPLPLMHHPTC VSWPPHPIPCWYHGSRHVPGLPATSSSMRSLGL LPEKWSLGPALVSQRLLLLAWNREPNIQFMSLP *RIIRRASP*LEGKRQDKKLSLRQPSHGPHSRTL LSTSFHVILLALMKNPYSSGFLELLPVPL*QAS PEVPPTTS*WRH*KTSRGIRFGKRLLPWATLST KA*TNLRMTRALTPTQFPIMPLEMSGNECLNQAL NCCASA*ALEVVISDVIHLDGAMTMV*TTRLER SGTVREKMAR**AAHVLGTEKENSSVTLMRQRV MMMGRHTT*ENSGRRNISVPFAPAHALEASGAGA VTTAADLGVNPVPKALLASPTTSILRDTIREQT LMLIAQLSASCL*MYRLTEKIPESKSSFQSRGT SMSLCQDPSKLE*C*QTQLRVLLSFLSPLLWRKF SSFSSTHSFSKHHPGSFLRVFS*MRAAHCLFCF EVFNTAQYFK*SDSKIWFGINRKAYAANQDANV LK*YDQNFK*ESHPNTSAFT*VSGPQYCRNKHDL VTVIF*ISTVLTFSK*S**LPRNIFLLPVIYQF FPVFLYGKNCIENT*YAVDKRNLV*LWWVIIFY TVCAKALLLWKDNCFNKRFTFHNLKFIYLI*DTF GGNNSCEYSFSIQQTFENL*CASLIVDFSTRFS KSVATKTDWFLSLHLFTNGDSFTLSALIDLSGP QYLLKLLVYRSEV**K*SLVALF*PL*FFPSSLH LSFIE*TSVQRDCLQGK*K*LRY*KYLNSIIWR SFILGKPHGMITPI*HVLLWVS*HFWHGLINSQ LNSLFTPDL*RSFLFQFNIEETNFGIKFDIQARR SGSHL*SQHFGRLRQVDHLRSGV*DQPGQHGET LSLLKIQMLPGMAVGVFGPGYSEG*GRRMA*TQ KAEVAVS*DCAIELQHGGQSKTPSQKT*INKCDI QNSK*WKWK*LLVKN**FIINKTCKALFKYTNV Q*KKVFQ*TPGKEY*N*IQKDTSSCSFEKKKY* LPFELIYSL*NALTFSYLI*GRF*SLILQGMKLR *GFLSEIQTQPLDFYLLLIFKLYQPNSHQESCS VCFLKITESTK*R*Y*TTV

Sequence FN1.gAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCA CACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGA AATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACA AGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCT GGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAG CTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCC ACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACA TCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAAC CGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGACGAGCTTCCCCAACTGGTAACCCTT CCACACCCCAATCTTCATGGACCAGAGATCTTGGATGTTCCTTCCACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTA TTCAGCTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCAC CCCCATAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAG TACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAG GTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTT GAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTG TTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGG ACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGA TGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGAC AACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATG TTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGT CTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAG CTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTA TTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAAT GATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTAC TGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCA GTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTAT GTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGG AAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCA GTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATT TATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTC CTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTT ATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACA GTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGG CTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCT CTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGG AGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAA TAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGA AAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCAT TTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGG TTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGT TTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNHYSCTN*PEVHSGH THKPERPVDTTQCSAHWISSAGDPQGEDRTNER NQPCS*QLIRGCIRTYGGHQI*SECLCS*GHFD KQTSSGSCHHSGECQPTKKGSCDRCY*DHHHH*L ENQD*DDHWLPS*CRSSQWPDSNPENHQARCQK LHHHRFTTRH*LQDLPVHLE*QCSELPCGHRRL HCH*CTIQPAFPGHHTQFLAGIMAAATCQDYRLH HQV*EAWVSSQRSGPSAPPWCHRGYYYWPGTGN RIYNLCHCPEE*SEERAPDWKEKDRRASPTGNP STPQSSWTRDLGCSFHSSKDPFRHPPWV*HWKWY SASWHFWSATQCWATNDL*GTWF*ADHTAHNGH PHKA*AKTIPAECRTRSSLSDNHLMGPIPGHF* VHHFMSSCWH**RTLTVQGSWNFYQCHSDRPHQR CHLQRHSGGTERPAEA*GSGRGCYRGQLCQRRL EPTYG*LVL*PLHSFPLCRWR*VGTNV*IRL*T VVPVLRLWKWSFQM*FI*MVP*QWCELQDWREVG PSGRKWPDDELHMSWERKRRIQV*PS*GNVL** WEDIPRRRTVAEGISRCHLLLHMLWRPAGLAL* QLPQTWG*TQSRRHYWPVLQPVFSEIPSENKH*C *LPN*VLHAFRCTG*QRRFPRVNHLSNPEEQAC LSAKIHLNWSDVSRPSLEFFFLS*ALCSGGSSP ASAQLTASPSITLGVS*GFSHK*GLHIACSASKY SIPLSILNEVILRFGLGSIGKHMQPTKMQMF*N DMTKILSRKVTQTLLLSLKCLARNTVGTSMILL L*YFKYPQYSLFPNDPSNCLEISFSYLLFINFSQ YFYTEKIVLKTLSMQLIRGIWYNYGG*LFFILY VPKLYYCGKTTVLIKDLHSTT*SSSI*YKTPSG EIIPVNILFQFSKHLKIYDVQV*LLISVQDFLNQ LLQKLIGFCHFISSLMEIALHFLL**I*VDPNI Y*NC*FTVQKYNRNNL*LLFSNHCNSSLLPSTF PSLNKPLFKEIACKGNKND*DIKSI*IV*YGGVL S*GNPMV**PPSNMSYFGSADTFGMA**TPS*T VCLLLICKGHFFFNLTLRKQTLGLNLTFRPGAV AHTSNPSTLGG*GRWIT*GQEFETSLANMVKPCL Y*KYKCYQAWQWASLVPATQKAEVGEWLEPRRR RLQ*AEIVPLNSSMGDRARLHPKKHK*INVTFR IVNSGNGNNY**KISSS**TRHAKHFLNILMYNE RRYFNKHLAKNIKTKYRKILPHVHLKKKNTNSH LN*FTVYKML*HLAISFRGGFRA*FYRA*SSDE VSLAKYRPSPWTSIYY*SLSFISPIVTKRAALYV F*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQV TPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKE INLAPDSSSVVVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITIS WRTKTETITGFQVDAVPANGQTPIQRTIKPDVRS YTITGLQPGTDYKIYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLLVSWQPPRARITGY IIKYEKPGSPPREVVPRPRPGVTEATITGLEPGT EYTIYVIALKNNQKSEPLIGRKKTDELPQLVTL PHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNG IQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTAT PIRHRPRPYPPNVGQEALSQTTISWAPFQDTSE YIISCHPVGTDEEPLQFRVPGTSTSATLTGLTR GATYNVIVEALKDQQRHKVREEVVTVGNSVNEGL NQPTDDSCFDPYTVSHYAVGDEWERMSESGFKL LCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKW DRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDD GKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCD NCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTN VNCPIECFMPLDVQADREDSRE*IIFPIQRNKHV SLPRSI*TGVMLADPA*SSSFFLKPFALEEVLQ LQLNSQLLQASPWEFPEGFLINEGCTLPVLLRS IQYRSVF*MK*F*DLVWDQ*ESICSQPRCKCFEM I*PKF*VGKSPKHFCFHLSVWPAIL*EQA*SCY CDILNIHSTHFFQMILVIA*KYLSLTCYLSIFP SIFIRKKLY*KHLVCS**EEFGIIMVGDYFLYCM CQSFTTVERQLF**KIYIPQLEVHLFDIRHLRG K*FL*IFFFNSANI*KSMMCKSNC*FQYKIF*I SCYKN*LVFVTSSLH*WR*LYTFCFNRFKWTPIF IKIASLPFRSIIEIIFSCSFLTIVILPFFPPPF LH*INLCSKRLPAREIKMTKILKVFE*YNMEEF YLRETPWYDNPHLTCLTLGQLTLLAWLDKLPAKQ FVYS*SVKVISFSI*H*GNKLWD*I*HSGQAQW LTPLIPALWEAEAGGSPEVRSLRPAWPTW*NLV SIKNTNVTRHGSGRLWSRLLRRLR*ENGLNPEGG GCSELRLCH*TPAWGTEQDSIPKNINK*M*HSE **IVEMEIIISKKLVVHNKQDMQSTF*IY*CTM KEGISINTWQRILKLNTERYFLMFI*KKKILTPI *IDLQFIKCFDI*LSHLGEVLELNFTGHEAQMR FP*RNTDPAPGLLFITDL*ALSAQ*SPRELLCM FFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PLFLHQLT*SSLRS HPQA*APSGHHPMFSSLDIECG*PPRRRPDQ*K KSTLLLTAHPWLYQDLWWPPNMK*VSMLLRTL*Q ADQLRELSPLWRMSAHQEGLV*QMLLRPPSPLA GEPRLRRSLASKLMPFQPMARLQSREPSSQMSE ATPSQVYNQALTTRSTCTP*MTMLGAPLWSSTPP LPLMHHPTCVSWPPHPIPCWYHGSRHVPGLPAT SSSMRSLGLLPEKWSLGPALVSQRLLLLAWNRE PNIQFMSLP*RIIRRASP*LEGKRQTSFPNW*PF HTPIFMDQRSWMFLPQFKRPLSSPTLGMTLEMV FSFLALLVSNPVLGNK*SLRNMVLGGPHRPQRP PP*GIGQDHTRRM*DKKLSLRQPSHGPHSRTLLS TSFHVILLALMKNPYSSGFLELLPVPL*QASPE VPPTTS*WRH*KTSRGIRFGKRLLPWATLSTKA *TNLRMTRALTPTQFPIMPLEMSGNECLNQALNC CASA*ALEVVISDVIHLDGAMTMV*TTRLERSG TVREKMAR**AAHVLGTEKENSSVTLMRQRVMM MGRHTT*ENSGRRNISVPFAPAHALEASGAGAVT TAADLGVNPVPKALLASPTTSILRDTIREQTLM LIAQLSASCL*MYRLTEKIPESKSSFQSRGTSM SLCQDPSKLE*C*QTQLRVLLSFLSPLLWRKFSS FSSTHSFSKHHPGSFLRVFS*MRAAHCLFCFEV FNTAQYFK*SDSKIWFGINRKAYAANQDANVLK *YDQNFK*ESHPNTSAFT*VSGPQYCRNKHDLVT VIF*ISTVLTFSK*S**LPRNIFLLPVIYQFFP VFLYGKNCIENT*YAVDKRNLV*LWWVIIFYTV CAKALLLWKDNCFNKRFTFHNLKFIYLI*DTFGG NNSCEYSFSIQQTFENL*CASLIVDFSTRFSKS VATKTDWFLSLHLFTNGDSFTLSALIDLSGPQY LLKLLVYRSEV**K*SLVALF*PL*FFPSSLHLS FIE*TSVQRDCLQGK*K*LRY*KYLNSIIWRSF ILGKPHGMITPI*HVLLWVS*HFWHGLINSQLN SLFTPDL*RSFLFQFNIEETNFGIKFDIQARRSG SHL*SQHFGRLRQVDHLRSGV*DQPGQHGETLS LLKIQMLPGMAVGVFGPGYSEG*GRRMA*TQKA EVAVS*DCAIELQHGGQSKTPSQKT*INKCDIQN SK*WKWK*LLVKN**FIINKTCKALFKYTNVQ* KKVFQ*TPGKEY*N*IQKDTSSCSFEKKKY*LP FELIYSL*NALTFSYLI*GRF*SLILQGMKLR*G FLSEIQTQPLDFYLLLIFKLYQPNSHQESCSVC FLKITESTK*R*Y*TTV

Sequence FN1.fAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCA CACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGA AATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACA AGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCT GGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAG CTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCC ACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACA TCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAAC CGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGACGAGCTTCCCCAACTGGTAACCCTT CCACACCCCAATCTTCATGGACCAGAGATCTTGGATGTTCCTTCCACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTA TTCAGCTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCAC CCCCATAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGTGAGGAAATCCAAATTGGTCACATCCCCAGGGAAGATGTAGACTATCACCTGTACCCA CACGGTCCGGGACTCAATCCAAATGCCTCTACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCA TTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCAC CTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAA CCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCC AGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCA GGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAG ACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCC GCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTG CCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTG CCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGC TCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATA CCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGA CCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATA TTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTT TATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAA AGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAAT TCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTA CAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAA ATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATT GAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAG GGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTT TACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACAC CTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAA AAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGC AGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAA TAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGG TATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATT GATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTT AGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAA AAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNHYSCTN*PEVHSGH THKPERPVDTTQCSAHWISSAGDPQGEDRTNER NQPCS*QLIRGCIRTYGGHQI*SECLCS*GHFD KQTSSGSCHHSGECQPTKKGSCDRCY*DHHHH*L ENQD*DDHWLPS*CRSSQWPDSNPENHQARCQK LHHHRFTTRH*LQDLPVHLE*QCSELPCGHRRL HCH*CTIQPAFPGHHTQFLAGIMAAATCQDYRLH HQV*EAWVSSQRSGPSAPPWCHRGYYYWPGTGN RIYNLCHCPEE*SEERAPDWKEKDRRASPTGNP STPQSSWTRDLGCSFHSSKDPFRHPPWV*HWKWY SASWHFWSATQCWATNDL*GTWF*ADHTAHNGH PHKA*AKTIPAECR*GNPNWSHPQGRCRLSPVP TRSGTQSKCLYRTRSSLSDNHLMGPIPGHF*VHH FMSSCWH**RTLTVQGSWNFYQCHSDRPHQRCH LQRHSGGTERPAEA*GSGRGCYRGQLCQRRLEP TYG*LVL*PLHSFPLCRWR*VGTNV*IRL*TVVP VLRLWKWSFQM*FI*MVP*QWCELQDWREVGPS GRKWPDDELHMSWERKRRIQV*PS*GNVL**WE DIPRRRTVAEGISRCHLLLHMLWRPAGLAL*QLP QTWG*TQSRRHYWPVLQPVFSEIPSENKH*C*L PN*VLHAFRCTG*QRRFPRVNHLSNPEEQACLS AKIHLNWSDVSRPSLEFFFLS*ALCSGGSSPASA QLTASPSITLGVS*GFSHK*GLHIACSASKYSI PLSILNEVILRFGLGSIGKHMQPTKMQMF*NDM TKILSRKVTQTLLLSLKCLARNTVGTSMILLL*Y FKYPQYSLFPNDPSNCLEISFSYLLFINFSQYF YTEKIVLKTLSMQLIRGIWYNYGG*LFFILYVP KLYYCGKTTVLIKDLHSTT*SSSI*YKTPSGEII PVNILFQFSKHLKIYDVQV*LLISVQDFLNQLL QKLIGFCHFISSLMEIALHFLL**I*VDPNIY* NC*FTVQKYNRNNL*LLFSNHCNSSLLPSTFPSL NKPLFKEIACKGNKND*DIKSI*IV*YGGVLS* GNPMV**PPSNMSYFGSADTFGMA**TPS*TVC LLLICKGHFFFNLTLRKQTLGLNLTFRPGAVAHT SNPSTLGG*GRWIT*GQEFETSLANMVKPCLY* KYKCYQAWQWASLVPATQKAEVGEWLEPRRRRL Q*AEIVPLNSSMGDRARLHPKKHK*INVTFRIVN SGNGNNY**KISSS**TRHAKHFLNILMYNERR YFNKHLAKNIKTKYRKILPHVHLKKKNTNSHLN *FTVYKML*HLAISFRGGFRA*FYRA*SSDEVSL AKYRPSPWTSIYY*SLSFISPIVTKRAALYVF* KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQV TPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKE INLAPDSSSVVVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITIS WRTKTETITGFQVDAVPANGQTPIQRTIKPDVRS YTITGLQPGTDYKIYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLLVSWQPPRARITGY IIKYEKPGSPPREVVPRPRPGVTEATITGLEPGT EYTIYVIALKNNQKSEPLIGRKKTDELPQLVTL PHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNG IQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTAT PIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYP HGPGLNPNASTGQEALSQTTISWAPFQDTSEYI ISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGAT YNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQ PTDDSCFDPYTVSHYAVGDEWERMSESGFKLLC QCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQ GENGQMMSCTCLGNGKGEFKCDPHEATCYDDGK TYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNC RRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNC PIECFMPLDVQADREDSRE*IIFPIQRNKHVSL PRSI*TGVMLADPA*SSSFFLKPFALEEVLQLQ LNSQLLQASPWEFPEGFLINEGCTLPVLLRSIQY RSVF*MK*F*DLVWDQ*ESICSQPRCKCFEMI* PKF*VGKSPKHFCFHLSVWPAIL*EQA*SCYCD ILNIHSTHFFQMILVIA*KYLSLTCYLSIFPSIF IRKKLY*KHLVCS**EEFGIIMVGDYFLYCMCQ SFTTVERQLF**KIYIPQLEVHLFDIRHLRGK* FL*IFFFNSANI*KSMMCKSNC*FQYKIF*ISCY KN*LVFVTSSLH*WR*LYTFCFNRFKWTPIFIK IASLPFRSIIEIIFSCSFLTIVILPFFPPPFLH *INLCSKRLPAREIKMTKILKVFE*YNMEEFYLR ETPWYDNPHLTCLTLGQLTLLAWLDKLPAKQFV YS*SVKVISFSI*H*GNKLWD*I*HSGQAQWLT PLIPALWEAEAGGSPEVRSLRPAWPTW*NLVSIK NTNVTRHGSGRLWSRLLRRLR*ENGLNPEGGGC SELRLCH*TPAWGTEQDSIPKNINK*M*HSE** IVEMEIIISKKLVVHNKQDMQSTF*IY*CTMKEG ISINTWQRILKLNTERYFLMFI*KKKILTPI*I DLQFIKCFDI*LSHLGEVLELNFTGHEAQMRFP *RNTDPAPGLLFITDL*ALSAQ*SPRELLCMFFK NHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PLFLHQLT*SSLRS HPQA*APSGHHPMFSSLDIECG*PPRRRPDQ*K KSTLLLTAHPWLYQDLWWPPNMK*VSMLLRTL*Q ADQLRELSPLWRMSAHQEGLV*QMLLRPPSPLA GEPRLRRSLASKLMPFQPMARLQSREPSSQMSE ATPSQVYNQALTTRSTCTP*MTMLGAPLWSSTPP LPLMHHPTCVSWPPHPIPCWYHGSRHVPGLPAT SSSMRSLGLLPEKWSLGPALVSQRLLLLAWNRE PNIQFMSLP*RIIRRASP*LEGKRQTSFPNW*PF HTPIFMDQRSWMFLPQFKRPLSSPTLGMTLEMV FSFLALLVSNPVLGNK*SLRNMVLGGPHRPQRP PP*GIGQDHTRRM*VRKSKLVTSPGKM*TITCTH TVRDSIQMPLQDKKLSLRQPSHGPHSRTLLSTS FHVILLALMKNPYSSGFLELLPVPL*QASPEVP PTTS*WRH*KTSRGIRFGKRLLPWATLSTKA*TN LRMTRALTPTQFPIMPLEMSGNECLNQALNCCA SA*ALEVVISDVIHLDGAMTMV*TTRLERSGTV REKMAR**AAHVLGTEKENSSVTLMRQRVMMMGR HTT*ENSGRRNISVPFAPAHALEASGAGAVTTA ADLGVNPVPKALLASPTTSILRDTIREQTLMLI AQLSASCL*MYRLTEKIPESKSSFQSRGTSMSLC QDPSKLE*C*QTQLRVLLSFLSPLLWRKFSSFS STHSFSKHHPGSFLRVFS*MRAAHCLFCFEVFN TAQYFK*SDSKIWFGINRKAYAANQDANVLK*YD QNFK*ESHPNTSAFT*VSGPQYCRNKHDLVTVI F*ISTVLTFSK*S**LPRNIFLLPVIYQFFPVF LYGKNCIENT*YAVDKRNLV*LWWVIIFYTVCAK ALLLWKDNCFNKRFTFHNLKFIYLI*DTFGGNN SCEYSFSIQQTFENL*CASLIVDFSTRFSKSVA TKTDWFLSLHLFTNGDSFTLSALIDLSGPQYLLK LLVYRSEV**K*SLVALF*PL*FFPSSLHLSFI E*TSVQRDCLQGK*K*LRY*KYLNSIIWRSFIL GKPHGMITPI*HVLLWVS*HFWHGLINSQLNSLF TPDL*RSFLFQFNIEETNFGIKFDIQARRSGSH L*SQHFGRLRQVDHLRSGV*DQPGQHGETLSLL KIQMLPGMAVGVFGPGYSEG*GRRMA*TQKAEVA VS*DCAIELQHGGQSKTPSQKT*INKCDIQNSK *WKWK*LLVKN**FIINKTCKALFKYTNVQ*KK VFQ*TPGKEY*N*IQKDTSSCSFEKKKY*LPFEL IYSL*NALTFSYLI*GRF*SLILQGMKLR*GFL SEIQTQPLDFYLLLIFKLYQPNSHQESCSVCFL KITESTK*R*Y*TTV

Sequence FN1.eAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGG ATGTCGATTCCATCAAAATTGCTTGGGAAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATT CCCTGCACCTGATGGTGAAGAAGACACTGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAG AGCCAGCCCCTGATTGGAACCCAGTCCACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACAC CACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGT GGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACT CTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCT TCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCAC TGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGT TTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTC CCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAA GAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGACGAGCTTCCCCAACTGGTAACCCTTCCACACCCCAATCTTCATGGACCAGAGATC TTGGATGTTCCTTCCACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAAC CCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCC GCCGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACT GATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCAC TGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGA CCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGT CATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGA GCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTG GCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGT CCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTT TAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGT GATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCA TCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGT GATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTC ACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCAC TTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAA AACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACA ACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAAT TCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCA CTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAG TATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGA TTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACC CCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCAT TTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAG GCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATG GCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTG AACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATT AGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAA GAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTT TGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCT GGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAA AGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNQH*SP*RTGIH*CG CRFHQNCLGKPTGASFQVQGDLLEP*GWNP*AI PCT*W*RRHCRAARPQTGF*VHSQCGCLAR*YG EPAPDWNPVHSYSCTN*PEVHSGHTHKPERPVDT TQCSAHWISSAGDPQGEDRTNERNQPCS*QLIR GCIRTYGGHQI*SECLCS*GHFDKQTSSGSCHH SGECQPTKKGSCDRCY*DHHHH*LENQD*DDHWL PS*CRSSQWPDSNPENHQARCQKLHHHRFTTRH *LQDLPVHLE*QCSELPCGHRRLHCH*CTIQPA FPGHHTQFLAGIMAAATCQDYRLHHQV*EAWVSS QRSGPSAPPWCHRGYYYWPGTGNRIYNLCHCPE E*SEERAPDWKEKDRRASPTGNPSTPQSSWTRD LGCSFHSSKDPFRHPPWV*HWKWYSASWHFWSAT QCWATNDL*GTWF*ADHTAHNGHPHKA*AKTIP AECRTRSSLSDNHLMGPIPGHF*VHHFMSSCWH **RTLTVQGSWNFYQCHSDRPHQRCHLQRHSGGT ERPAEA*GSGRGCYRGQLCQRRLEPTYG*LVL* PLHSFPLCRWR*VGTNV*IRL*TVVPVLRLWKW SFQM*FI*MVP*QWCELQDWREVGPSGRKWPDDE LHMSWERKRRIQV*PS*GNVL**WEDIPRRRTV AEGISRCHLLLHMLWRPAGLAL*QLPQTWG*TQ SRRHYWPVLQPVFSEIPSENKH*C*LPN*VLHAF RCTG*QRRFPRVNHLSNPEEQACLSAKIHLNWS DVSRPSLEFFFLS*ALCSGGSSPASAQLTASPS ITLGVS*GFSHK*GLHIACSASKYSIPLSILNEV ILRFGLGSIGKHMQPTKMQMF*NDMTKILSRKV TQTLLLSLKCLARNTVGTSMILLL*YFKYPQYS LFPNDPSNCLEISFSYLLFINFSQYFYTEKIVLK TLSMQLIRGIWYNYGG*LFFILYVPKLYYCGKT TVLIKDLHSTT*SSSI*YKTPSGEIIPVNILFQ FSKHLKIYDVQV*LLISVQDFLNQLLQKLIGFCH FISSLMEIALHFLL**I*VDPNIY*NC*FTVQK YNRNNL*LLFSNHCNSSLLPSTFPSLNKPLFKE IACKGNKND*DIKSI*IV*YGGVLS*GNPMV**P PSNMSYFGSADTFGMA**TPS*TVCLLLICKGH FFFNLTLRKQTLGLNLTFRPGAVAHTSNPSTLG G*GRWIT*GQEFETSLANMVKPCLY*KYKCYQAW QWASLVPATQKAEVGEWLEPRRRRLQ*AEIVPL NSSMGDRARLHPKKHK*INVTFRIVNSGNGNNY **KISSS**TRHAKHFLNILMYNERRYFNKHLAK NIKTKYRKILPHVHLKKKNTNSHLN*FTVYKML *HLAISFRGGFRA*FYRA*SSDEVSLAKYRPSP WTSIYY*SLSFISPIVTKRAALYVF*KSQKVQNK GDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDV DVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELF PAPDGEEDTAELQGLRPGSEYTVSVVALHDDME SQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWT PPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSV VVSGLMVATKYEVSVYALKDTLTSRPAQGVVTT LENVSPPRRARVTDATETTITISWRTKTETITG FQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGT DYKIYLYTLNDNARSSPVVIDASTAIDAPSNLR FLATTPNSLLVSWQPPRARITGYIIKYEKPGSP PREVVPRPRPGVTEATITGLEPGTEYTIYVIALK NNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEI LDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQ PSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYP PNVGQEALSQTTISWAPFQDTSEYIISCHPVGT DEEPLQFRVPGTSTSATLTGLTRGATYNVIVEA LKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFD PYTVSHYAVGDEWERMSESGFKLLCQCLGFGSG HFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMM SCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQW QKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPS PEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMP LDVQADREDSRE*IIFPIQRNKHVSLPRSI*TGV MLADPA*SSSFFLKPFALEEVLQLQLNSQLLQA SPWEFPEGFLINEGCTLPVLLRSIQYRSVF*MK *F*DLVWDQ*ESICSQPRCKCFEMI*PKF*VGKS PKHFCFHLSVWPAIL*EQA*SCYCDILNIHSTH FFQMILVIA*KYLSLTCYLSIFPSIFIRKKLY* KHLVCS**EEFGIIMVGDYFLYCMCQSFTTVERQ LF**KIYIPQLEVHLFDIRHLRGK*FL*IFFFN SANI*KSMMCKSNC*FQYKIF*ISCYKN*LVFV TSSLH*WR*LYTFCFNRFKWTPIFIKIASLPFRS IIEIIFSCSFLTIVILPFFPPPFLH*INLCSKR LPAREIKMTKILKVFE*YNMEEFYLRETPWYDN PHLTCLTLGQLTLLAWLDKLPAKQFVYS*SVKVI SFSI*H*GNKLWD*I*HSGQAQWLTPLIPALWE AEAGGSPEVRSLRPAWPTW*NLVSIKNTNVTRH GSGRLWSRLLRRLR*ENGLNPEGGGCSELRLCH* TPAWGTEQDSIPKNINK*M*HSE**IVEMEIII SKKLVVHNKQDMQSTF*IY*CTMKEGISINTWQ RILKLNTERYFLMFI*KKKILTPI*IDLQFIKCF DI*LSHLGEVLELNFTGHEAQMRFP*RNTDPAP GLLFITDL*ALSAQ*SPRELLCMFFKNHRKYKI KVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PTLIALKDWHSLMW MSIPSKLLGKAHRGKFPGTG*PTRALRMESMSY SLHLMVKKTLQSCKASDRVLSTQSVWLPCTMIWR ASP*LEPSPQLFLHQLT*SSLRSHPQA*APSGH HPMFSSLDIECG*PPRRRPDQ*KKSTLLLTAHP WLYQDLWWPPNMK*VSMLLRTL*QADQLRELSPL WRMSAHQEGLV*QMLLRPPSPLAGEPRLRRSLA SKLMPFQPMARLQSREPSSQMSEATPSQVYNQA LTTRSTCTP*MTMLGAPLWSSTPPLPLMHHPTCV SWPPHPIPCWYHGSRHVPGLPATSSSMRSLGLL PEKWSLGPALVSQRLLLLAWNREPNIQFMSLP* RIIRRASP*LEGKRQTSFPNW*PFHTPIFMDQRS WMFLPQFKRPLSSPTLGMTLEMVFSFLALLVSN PVLGNK*SLRNMVLGGPHRPQRPPP*GIGQDHT RRM*DKKLSLRQPSHGPHSRTLLSTSFHVILLAL MKNPYSSGFLELLPVPL*QASPEVPPTTS*WRH *KTSRGIRFGKRLLPWATLSTKA*TNLRMTRAL TPTQFPIMPLEMSGNECLNQALNCCASA*ALEVV ISDVIHLDGAMTMV*TTRLERSGTVREKMAR** AAHVLGTEKENSSVTLMRQRVMMMGRHTT*ENS GRRNISVPFAPAHALEASGAGAVTTAADLGVNPV PKALLASPTTSILRDTIREQTLMLIAQLSASCL *MYRLTEKIPESKSSFQSRGTSMSLCQDPSKLE *C*QTQLRVLLSFLSPLLWRKFSSFSSTHSFSKH HPGSFLRVFS*MRAAHCLFCFEVFNTAQYFK*S DSKIWFGINRKAYAANQDANVLK*YDQNFK*ES HPNTSAFT*VSGPQYCRNKHDLVTVIF*ISTVLT FSK*S**LPRNIFLLPVIYQFFPVFLYGKNCIE NT*YAVDKRNLV*LWWVIIFYTVCAKALLLWKD NCFNKRFTFHNLKFIYLI*DTFGGNNSCEYSFSI QQTFENL*CASLIVDFSTRFSKSVATKTDWFLS LHLFTNGDSFTLSALIDLSGPQYLLKLLVYRSE V**K*SLVALF*PL*FFPSSLHLSFIE*TSVQRD CLQGK*K*LRY*KYLNSIIWRSFILGKPHGMIT PI*HVLLWVS*HFWHGLINSQLNSLFTPDL*RS FLFQFNIEETNFGIKFDIQARRSGSHL*SQHFGR LRQVDHLRSGV*DQPGQHGETLSLLKIQMLPGM AVGVFGPGYSEG*GRRMA*TQKAEVAVS*DCAI ELQHGGQSKTPSQKT*INKCDIQNSK*WKWK*LL VKN**FIINKTCKALFKYTNVQ*KKVFQ*TPGK EY*N*IQKDTSSCSFEKKKY*LPFELIYSL*NA LTFSYLI*GRF*SLILQGMKLR*GFLSEIQTQPL DFYLLLIFKLYQPNSHQESCSVCFLKITESTK* R*Y*TTV

Sequence FN1.dAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGG ATGTCGATTCCATCAAAATTGCTTGGGAAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATT CCCTGCACCTGATGGTGAAGAAGACACTGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAG AGCCAGCCCCTGATTGGAACCCAGTCCACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACAC CACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGT GGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACT CTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCT TCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCAC TGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGT TTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTC CCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAA GAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAG CTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCA TAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACAT CATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCC ACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACC AACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTG CCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGT CAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGA AGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTG CCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAAT TGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTC TGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCA GCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAA TACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATAT GACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGA TATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATT TTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCC AAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATA ATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGC TACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTA AAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCA TTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTT AGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTG TTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCAC ACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATT AAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTT GCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTA AATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAA GGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAA TTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCC TTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTT AAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNQH*SP*RTGIH*CG CRFHQNCLGKPTGASFQVQGDLLEP*GWNP*AI PCT*W*RRHCRAARPQTGF*VHSQCGCLAR*YG EPAPDWNPVHSYSCTN*PEVHSGHTHKPERPVDT TQCSAHWISSAGDPQGEDRTNERNQPCS*QLIR GCIRTYGGHQI*SECLCS*GHFDKQTSSGSCHH SGECQPTKKGSCDRCY*DHHHH*LENQD*DDHWL PS*CRSSQWPDSNPENHQARCQKLHHHRFTTRH *LQDLPVHLE*QCSELPCGHRRLHCH*CTIQPA FPGHHTQFLAGIMAAATCQDYRLHHQV*EAWVSS QRSGPSAPPWCHRGYYYWPGTGNRIYNLCHCPE E*SEERAPDWKEKDSSKDPFRHPPWV*HWKWYS ASWHFWSATQCWATNDL*GTWF*ADHTAHNGHPH KA*AKTIPAECRTRSSLSDNHLMGPIPGHF*VH HFMSSCWH**RTLTVQGSWNFYQCHSDRPHQRC HLQRHSGGTERPAEA*GSGRGCYRGQLCQRRLEP TYG*LVL*PLHSFPLCRWR*VGTNV*IRL*TVV PVLRLWKWSFQM*FI*MVP*QWCELQDWREVGP SGRKWPDDELHMSWERKRRIQV*PS*GNVL**WE DIPRRRTVAEGISRCHLLLHMLWRPAGLAL*QL PQTWG*TQSRRHYWPVLQPVFSEIPSENKH*C* LPN*VLHAFRCTG*QRRFPRVNHLSNPEEQACLS AKIHLNWSDVSRPSLEFFFLS*ALCSGGSSPAS AQLTASPSITLGVS*GFSHK*GLHIACSASKYS IPLSILNEVILRFGLGSIGKHMQPTKMQMF*NDM TKILSRKVTQTLLLSLKCLARNTVGTSMILLL* YFKYPQYSLFPNDPSNCLEISFSYLLFINFSQY FYTEKIVLKTLSMQLIRGIWYNYGG*LFFILYVP KLYYCGKTTVLIKDLHSTT*SSSI*YKTPSGEI IPVNILFQFSKHLKIYDVQV*LLISVQDFLNQL LQKLIGFCHFISSLMEIALHFLL**I*VDPNIY* NC*FTVQKYNRNNL*LLFSNHCNSSLLPSTFPS LNKPLFKEIACKGNKND*DIKSI*IV*YGGVLS *GNPMV**PPSNMSYFGSADTFGMA**TPS*TVC LLLICKGHFFFNLTLRKQTLGLNLTFRPGAVAH TSNPSTLGG*GRWIT*GQEFETSLANMVKPCLY *KYKCYQAWQWASLVPATQKAEVGEWLEPRRRRL Q*AEIVPLNSSMGDRARLHPKKHK*INVTFRIV NSGNGNNY**KISSS**TRHAKHFLNILMYNER RYFNKHLAKNIKTKYRKILPHVHLKKKNTNSHLN *FTVYKML*HLAISFRGGFRA*FYRA*SSDEVS LAKYRPSPWTSIYY*SLSFISPIVTKRAALYVF *KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDV DVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELF PAPDGEEDTAELQGLRPGSEYTVSVVALHDDME SQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWT PPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSV VVSGLMVATKYEVSVYALKDTLTSRPAQGVVTT LENVSPPRRARVTDATETTITISWRTKTETITG FQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGT DYKIYLYTLNDNARSSPVVIDASTAIDAPSNLR FLATTPNSLLVSWQPPRARITGYIIKYEKPGSP PREVVPRPRPGVTEATITGLEPGTEYTIYVIALK NNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQ LPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATP IRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYI ISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGA TYNVIVEALKDQQRHKVREEVVTVGNSVNEGLN QPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLC QCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDR QGENGQMMSCTCLGNGKGEFKCDPHEATCYDDG KTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNC RRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVN CPIECFMPLDVQADREDSRE*IIFPIQRNKHVS LPRSI*TGVMLADPA*SSSFFLKPFALEEVLQLQ LNSQLLQASPWEFPEGFLINEGCTLPVLLRSIQ YRSVF*MK*F*DLVWDQ*ESICSQPRCKCFEMI *PKF*VGKSPKHFCFHLSVWPAIL*EQA*SCYCD ILNIHSTHFFQMILVIA*KYLSLTCYLSIFPSI FIRKKLY*KHLVCS**EEFGIIMVGDYFLYCMC QSFTTVERQLF**KIYIPQLEVHLFDIRHLRGK* FL*IFFFNSANI*KSMMCKSNC*FQYKIF*ISC YKN*LVFVTSSLH*WR*LYTFCFNRFKWTPIFI KIASLPFRSIIEIIFSCSFLTIVILPFFPPPFLH *INLCSKRLPAREIKMTKILKVFE*YNMEEFYL RETPWYDNPHLTCLTLGQLTLLAWLDKLPAKQF VYS*SVKVISFSI*H*GNKLWD*I*HSGQAQWLT PLIPALWEAEAGGSPEVRSLRPAWPTW*NLVSI KNTNVTRHGSGRLWSRLLRRLR*ENGLNPEGGG CSELRLCH*TPAWGTEQDSIPKNINK*M*HSE** IVEMEIIISKKLVVHNKQDMQSTF*IY*CTMKE GISINTWQRILKLNTERYFLMFI*KKKILTPI* IDLQFIKCFDI*LSHLGEVLELNFTGHEAQMRFP *RNTDPAPGLLFITDL*ALSAQ*SPRELLCMFF KNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PTLIALKDWHSLMW MSIPSKLLGKAHRGKFPGTG*PTRALRMESMSY SLHLMVKKTLQSCKASDRVLSTQSVWLPCTMIWR ASP*LEPSPQLFLHQLT*SSLRSHPQA*APSGH HPMFSSLDIECG*PPRRRPDQ*KKSTLLLTAHP WLYQDLWWPPNMK*VSMLLRTL*QADQLRELSPL WRMSAHQEGLV*QMLLRPPSPLAGEPRLRRSLA SKLMPFQPMARLQSREPSSQMSEATPSQVYNQA LTTRSTCTP*MTMLGAPLWSSTPPLPLMHHPTCV SWPPHPIPCWYHGSRHVPGLPATSSSMRSLGLL PEKWSLGPALVSQRLLLLAWNREPNIQFMSLP* RIIRRASP*LEGKRQFKRPLSSPTLGMTLEMVFS FLALLVSNPVLGNK*SLRNMVLGGPHRPQRPPP *GIGQDHTRRM*DKKLSLRQPSHGPHSRTLLST SFHVILLALMKNPYSSGFLELLPVPL*QASPEVP PTTS*WRH*KTSRGIRFGKRLLPWATLSTKA*T NLRMTRALTPTQFPIMPLEMSGNECLNQALNCC ASA*ALEVVISDVIHLDGAMTMV*TTRLERSGTV REKMAR**AAHVLGTEKENSSVTLMRQRVMMMG RHTT*ENSGRRNISVPFAPAHALEASGAGAVTT AADLGVNPVPKALLASPTTSILRDTIREQTLMLI AQLSASCL*MYRLTEKIPESKSSFQSRGTSMSL CQDPSKLE*C*QTQLRVLLSFLSPLLWRKFSSF SSTHSFSKHHPGSFLRVFS*MRAAHCLFCFEVFN TAQYFK*SDSKIWFGINRKAYAANQDANVLK*Y DQNFK*ESHPNTSAFT*VSGPQYCRNKHDLVTV IF*ISTVLTFSK*S**LPRNIFLLPVIYQFFPVF LYGKNCIENT*YAVDKRNLV*LWWVIIFYTVCA KALLLWKDNCFNKRFTFHNLKFIYLI*DTFGGN NSCEYSFSIQQTFENL*CASLIVDFSTRFSKSVA TKTDWFLSLHLFTNGDSFTLSALIDLSGPQYLL KLLVYRSEV**K*SLVALF*PL*FFPSSLHLSF IE*TSVQRDCLQGK*K*LRY*KYLNSIIWRSFIL GKPHGMITPI*HVLLWVS*HFWHGLINSQLNSL FTPDL*RSFLFQFNIEETNFGIKFDIQARRSGS HL*SQHFGRLRQVDHLRSGV*DQPGQHGETLSLL KIQMLPGMAVGVFGPGYSEG*GRRMA*TQKAEV AVS*DCAIELQHGGQSKTPSQKT*INKCDIQNS K*WKWK*LLVKN**FIINKTCKALFKYTNVQ*KK VFQ*TPGKEY*N*IQKDTSSCSFEKKKY*LPFE LIYSL*NALTFSYLI*GRF*SLILQGMKLR*GF LSEIQTQPLDFYLLLIFKLYQPNSHQESCSVCFL KITESTK*R*Y*TTV

Sequence FN1.cAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGAGGTGCCCCAACTCACTGACCTAAGCTTTGTTGA TATAACCGATTCAAGCATCGGCCTGAGGTGGACCCCGCTAAACTCTTCCACCATTATTGGGTACCGCATCACAGTAGTTGCGGCAGGAGAAGGTATCCCT ATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTCACAGGGCTGGAGCCGGGCATTGACTATGATATCAGCGTTATCACTCTCATTAATG GCGGCGAGAGTGCCCCTACTACACTGACACAACAAACGGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAACATTGGTCCAGACACCATGCGTGTCAC CTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGATGTTGCAGAGTTGTCAATTTCTCCTTCA GACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAACAACATGAGAGCACACCTCTTAGAGGAA GACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCACTGGATTGCTCCTCGAGCCACCATCAC TGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGGAATTCCATCACCCTCACCAACCTCACT CCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAACAATCAACAGTTTCTGATGTTCCGAGGG ACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATATTACAGGATCACTTACGGAGAGACAGG AGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCTGGAGTTGATTATACCATCACTGTGTAT GCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACAAACCATCCCAGATGCAAGTGACCGATG TTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCACTCCCAAAAATGGACCAGGACCAACAAA AACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTTAGTGTCTATGCTCAGAATCCAAGCGGA GAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCTTGGG AAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATTCCCTGCACCTGATGGTGAAGAAGACAC TGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAGAGCCAGCCCCTGATTGGAACCCAGTCC ACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATC GAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCAC CAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGG GCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATG GCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTT GAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTG CTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCC CTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGAT TGGAAGGAAAAAGACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAACCC AGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCCGC CGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGA TGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTG AAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACC CCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCA TTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGC TGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGC AGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCC CGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTA GATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGA TGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATC ACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGA TTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCAC CCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTT TTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAA CACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAAC TGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTC AGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACT TCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTA TAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATT GCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCC CATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTT CTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGC TGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGC AGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAA CTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAG TAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGA ATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTG ACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGG ACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAG GTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPRGAPTH*PKLC* YNRFKHRPEVDPAKLFHHYWVPHHSSCGRRRYP YF*RFCGLLSRILHSHRAGAGH*L*YQRYHSH*W RRECPYYTDTTNGCSSSH*PAIHQHWSRHHACH LGSTPIH*FNQLPGALLTCEK*GRCCRVVNFSF RQCSGLNKSPAWYRICSECLQCLRTT*EHTS*RK TENRS*FPNWH*LF*YYCQLFYCALDCSSSHHH WLQDPPSSRALQWETSRRSGAPLSEFHHPHQPH SRHRVCGQHRCS*WQRGKSLIDWPTINSF*CSEG PGSCCCDPHQPTDQLGCSCCHSEILQDHLRRDR RK*PCPGVHCAWEQVYSYHQRP*TWS*LYHHCV CCHWPWRQPRKQQANFH*LPNRN*QTIPDASDRC SGQQH*CQVAAFKFPCYWLQSNHHSQKWTRTNK N*NCRSRSNRNDY*RLAAHSGVCG*CLCSESKR RESASGSDCSNQH*SP*RTGIH*CGCRFHQNCLG KPTGASFQVQGDLLEP*GWNP*AIPCT*W*RRH CRAARPQTGF*VHSQCGCLAR*YGEPAPDWNPV HSYSCTN*PEVHSGHTHKPERPVDTTQCSAHWIS SAGDPQGEDRTNERNQPCS*QLIRGCIRTYGGH QI*SECLCS*GHFDKQTSSGSCHHSGECQPTKK GSCDRCY*DHHHH*LENQD*DDHWLPS*CRSSQW PDSNPENHQARCQKLHHHRFTTRH*LQDLPVHL E*QCSELPCGHRRLHCH*CTIQPAFPGHHTQFL AGIMAAATCQDYRLHHQV*EAWVSSQRSGPSAPP WCHRGYYYWPGTGNRIYNLCHCPEE*SEERAPD WKEKDSSKDPFRHPPWV*HWKWYSASWHFWSAT QCWATNDL*GTWF*ADHTAHNGHPHKA*AKTIPA ECRTRSSLSDNHLMGPIPGHF*VHHFMSSCWH* *RTLTVQGSWNFYQCHSDRPHQRCHLQRHSGGT ERPAEA*GSGRGCYRGQLCQRRLEPTYG*LVL*P LHSFPLCRWR*VGTNV*IRL*TVVPVLRLWKWS FQM*FI*MVP*QWCELQDWREVGPSGRKWPDDE LHMSWERKRRIQV*PS*GNVL**WEDIPRRRTVA EGISRCHLLLHMLWRPAGLAL*QLPQTWG*TQS RRHYWPVLQPVFSEIPSENKH*C*LPN*VLHAF RCTG*QRRFPRVNHLSNPEEQACLSAKIHLNWSD VSRPSLEFFFLS*ALCSGGSSPASAQLTASPSI TLGVS*GFSHK*GLHIACSASKYSIPLSILNEV ILRFGLGSIGKHMQPTKMQMF*NDMTKILSRKVT QTLLLSLKCLARNTVGTSMILLL*YFKYPQYSL FPNDPSNCLEISFSYLLFINFSQYFYTEKIVLK TLSMQLIRGIWYNYGG*LFFILYVPKLYYCGKTT VLIKDLHSTT*SSSI*YKTPSGEIIPVNILFQF SKHLKIYDVQV*LLISVQDFLNQLLQKLIGFCH FISSLMEIALHFLL**I*VDPNIY*NC*FTVQKY NRNNL*LLFSNHCNSSLLPSTFPSLNKPLFKEI ACKGNKND*DIKSI*IV*YGGVLS*GNPMV**P PSNMSYFGSADTFGMA**TPS*TVCLLLICKGHF FFNLTLRKQTLGLNLTFRPGAVAHTSNPSTLGG *GRWIT*GQEFETSLANMVKPCLY*KYKCYQAW QWASLVPATQKAEVGEWLEPRRRRLQ*AEIVPLN SSMGDRARLHPKKHK*INVTFRIVNSGNGNNY* *KISSS**TRHAKHFLNILMYNERRYFNKHLAK NIKTKYRKILPHVHLKKKNTNSHLN*FTVYKML* HLAISFRGGFRA*FYRA*SSDEVSLAKYRPSPW TSIYY*SLSFISPIVTKRAALYVF*KSQKVQNK GDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVD ITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIP IFEDFVDSSVGYYTVTGLEPGIDYDISVITLIN GGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVT WAPPPSIDLTNFLVRYSPVKNEEDVAELSISPS DNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRG RQKTGLDSPTGIDFSDITANSFTVHWIAPRATIT GYRIRHHPEHFSGRPREDRVPHSRNSITLTNLT PGTEYVVSIVALNGREESPLLIGQQSTVSDVPR DLEVVAATPTSLLISWDAPAVTVRYYRITYGETG GNSPVQEFTVPGSKSTATISGLKPGVDYTITVY AVTGRGDSPASSKPISINYRTEIDKPSQMQVTD VQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTK TKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSG ESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAW ESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDT AELQGLRPGSEYTVSVVALHDDMESQPLIGTQS TAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGY RVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVAT KYEVSVYALKDTLTSRPAQGVVTTLENVSPPRR ARVTDATETTITISWRTKTETITGFQVDAVPAN GQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTL NDNARSSPVVIDASTAIDAPSNLRFLATTPNSL LVSWQPPRARITGYIIKYEKPGSPPREVVPRPR PGVTEATITGLEPGTEYTIYVIALKNNQKSEPLI GRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQP SVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYP PNVGQEALSQTTISWAPFQDTSEYIISCHPVGTD EEPLQFRVPGTSTSATLTGLTRGATYNVIVEAL KDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFD PYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGH FRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMS CTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQW QKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSP EGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPL DVQADREDSRE*IIFPIQRNKHVSLPRSI*TGV MLADPA*SSSFFLKPFALEEVLQLQLNSQLLQAS PWEFPEGFLINEGCTLPVLLRSIQYRSVF*MK* F*DLVWDQ*ESICSQPRCKCFEMI*PKF*VGKS PKHFCFHLSVWPAIL*EQA*SCYCDILNIHSTHF FQMILVIA*KYLSLTCYLSIFPSIFIRKKLY*K HLVCS**EEFGIIMVGDYFLYCMCQSFTTVERQ LF**KIYIPQLEVHLFDIRHLRGK*FL*IFFFNS ANI*KSMMCKSNC*FQYKIF*ISCYKN*LVFVT SSLH*WR*LYTFCFNRFKWTPIFIKIASLPFRS IIEIIFSCSFLTIVILPFFPPPFLH*INLCSKRL PAREIKMTKILKVFE*YNMEEFYLRETPWYDNP HLTCLTLGQLTLLAWLDKLPAKQFVYS*SVKVI SFSI*H*GNKLWD*I*HSGQAQWLTPLIPALWEA EAGGSPEVRSLRPAWPTW*NLVSIKNTNVTRHG SGRLWSRLLRRLR*ENGLNPEGGGCSELRLCH* TPAWGTEQDSIPKNINK*M*HSE**IVEMEIIIS KKLVVHNKQDMQSTF*IY*CTMKEGISINTWQR ILKLNTERYFLMFI*KKKILTPI*IDLQFIKCF DI*LSHLGEVLELNFTGHEAQMRFP*RNTDPAPG LLFITDL*ALSAQ*SPRELLCMFFKNHRKYKIK VILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQRCPNSLT*ALL I*PIQASA*GGPR*TLPPLLGTASQ*LRQEKVSL FLKILWTPQ*DTTQSQGWSRALTMISALSLSLM AARVPLLH*HNKRLFLLPLTCDSPTLVQTPCVS PGLHPHPLI*PTSWCVTHL*KMRKMLQSCQFLLQ TMQWS*QISCLVQNM**VSPVSTNNMRAHLLEE DRKQVLIPQLALTFLILLPTLLLCTGLLLEPPS LATGSAIIPSTSVGDLEKIGCPTLGIPSPSPTSL QAQSMWSASLLLMAERKVPY*LANNQQFLMFRG TWKLLLRPPPAY*SAGMLLLSQ*DITGSLTERQ EEIALSRSSLCLGASLQLPSAALNLELIIPSLCM LSLAVETAPQAASQFPLITEQKLTNHPRCK*PM FRTTALVSSGCLQVPLLLVTE*PPLPKMDQDQQ KLKLQVQIKQK*LLKACSPQWSMWLVSMLRIQAE RVSLWFRLQ*PTLIALKDWHSLMWMSIPSKLLG KAHRGKFPGTG*PTRALRMESMSYSLHLMVKKT LQSCKASDRVLSTQSVWLPCTMIWRASP*LEPSP QLFLHQLT*SSLRSHPQA*APSGHHPMFSSLDI ECG*PPRRRPDQ*KKSTLLLTAHPWLYQDLWWP PNMK*VSMLLRTL*QADQLRELSPLWRMSAHQEG LV*QMLLRPPSPLAGEPRLRRSLASKLMPFQPM ARLQSREPSSQMSEATPSQVYNQALTTRSTCTP *MTMLGAPLWSSTPPLPLMHHPTCVSWPPHPIPC WYHGSRHVPGLPATSSSMRSLGLLPEKWSLGPA LVSQRLLLLAWNREPNIQFMSLP*RIIRRASP* LEGKRQFKRPLSSPTLGMTLEMVFSFLALLVSNP VLGNK*SLRNMVLGGPHRPQRPPP*GIGQDHTR RM*DKKLSLRQPSHGPHSRTLLSTSFHVILLAL MKNPYSSGFLELLPVPL*QASPEVPPTTS*WRH* KTSRGIRFGKRLLPWATLSTKA*TNLRMTRALT PTQFPIMPLEMSGNECLNQALNCCASA*ALEVV ISDVIHLDGAMTMV*TTRLERSGTVREKMAR**A AHVLGTEKENSSVTLMRQRVMMMGRHTT*ENSG RRNISVPFAPAHALEASGAGAVTTAADLGVNPV PKALLASPTTSILRDTIREQTLMLIAQLSASCL* MYRLTEKIPESKSSFQSRGTSMSLCQDPSKLE* C*QTQLRVLLSFLSPLLWRKFSSFSSTHSFSKH HPGSFLRVFS*MRAAHCLFCFEVFNTAQYFK*SD SKIWFGINRKAYAANQDANVLK*YDQNFK*ESH PNTSAFT*VSGPQYCRNKHDLVTVIF*ISTVLT FSK*S**LPRNIFLLPVIYQFFPVFLYGKNCIEN T*YAVDKRNLV*LWWVIIFYTVCAKALLLWKDN CFNKRFTFHNLKFIYLI*DTFGGNNSCEYSFSI QQTFENL*CASLIVDFSTRFSKSVATKTDWFLSL HLFTNGDSFTLSALIDLSGPQYLLKLLVYRSEV **K*SLVALF*PL*FFPSSLHLSFIE*TSVQRD CLQGK*K*LRY*KYLNSIIWRSFILGKPHGMITP I*HVLLWVS*HFWHGLINSQLNSLFTPDL*RSF LFQFNIEETNFGIKFDIQARRSGSHL*SQHFGR LRQVDHLRSGV*DQPGQHGETLSLLKIQMLPGMA VGVFGPGYSEG*GRRMA*TQKAEVAVS*DCAIE LQHGGQSKTPSQKT*INKCDIQNSK*WKWK*LL VKN**FIINKTCKALFKYTNVQ*KKVFQ*TPGKE Y*N*IQKDTSSCSFEKKKY*LPFELIYSL*NAL TFSYLI*GRF*SLILQGMKLR*GFLSEIQTQPL DFYLLLIFKLYQPNSHQESCSVCFLKITESTK*R *Y*TTV

Sequence FN1.bAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGAGGTGCCCCAACTCACTGACCTAAGCTTTGTTGA TATAACCGATTCAAGCATCGGCCTGAGGTGGACCCCGCTAAACTCTTCCACCATTATTGGGTACCGCATCACAGTAGTTGCGGCAGGAGAAGGTATCCCT ATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTCACAGGGCTGGAGCCGGGCATTGACTATGATATCAGCGTTATCACTCTCATTAATG GCGGCGAGAGTGCCCCTACTACACTGACACAACAAACGGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAACATTGGTCCAGACACCATGCGTGTCAC CTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGATGTTGCAGAGTTGTCAATTTCTCCTTCA GACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAACAACATGAGAGCACACCTCTTAGAGGAA GACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCACTGGATTGCTCCTCGAGCCACCATCAC TGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGGAATTCCATCACCCTCACCAACCTCACT CCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAACAATCAACAGTTTCTGATGTTCCGAGGG ACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATATTACAGGATCACTTACGGAGAGACAGG AGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCTGGAGTTGATTATACCATCACTGTGTAT GCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACAAACCATCCCAGATGCAAGTGACCGATG TTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCACTCCCAAAAATGGACCAGGACCAACAAA AACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTTAGTGTCTATGCTCAGAATCCAAGCGGA GAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCTTGGG AAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATTCCCTGCACCTGATGGTGAAGAAGACAC TGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAGAGCCAGCCCCTGATTGGAACCCAGTCC ACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATC GAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCAC CAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGG GCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATG GCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTT GAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTG CTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCC CTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGAT TGGAAGGAAAAAGACAGACGAGCTTCCCCAACTGGTAACCCTTCCACACCCCAATCTTCATGGACCAGAGATCTTGGATGTTCCTTCCACAGTTCAAAAG ACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTG AGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGACAAGAAGCTCTCTC TCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTT CCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTC GGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGT TGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGG TGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAG GAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTG CTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTAC AACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATT CCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTC TTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTT CTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAAT AGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAG TGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCC TAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGG AATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCC ACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATG TGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGC TTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTC TTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACT AAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTC AGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAA CAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTC AGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTAC TCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGA CTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAA CAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGAT ACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGA GGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAA GCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPRGAPTH*PKLC* YNRFKHRPEVDPAKLFHHYWVPHHSSCGRRRYP YF*RFCGLLSRILHSHRAGAGH*L*YQRYHSH*W RRECPYYTDTTNGCSSSH*PAIHQHWSRHHACH LGSTPIH*FNQLPGALLTCEK*GRCCRVVNFSF RQCSGLNKSPAWYRICSECLQCLRTT*EHTS*RK TENRS*FPNWH*LF*YYCQLFYCALDCSSSHHH WLQDPPSSRALQWETSRRSGAPLSEFHHPHQPH SRHRVCGQHRCS*WQRGKSLIDWPTINSF*CSEG PGSCCCDPHQPTDQLGCSCCHSEILQDHLRRDR RK*PCPGVHCAWEQVYSYHQRP*TWS*LYHHCV CCHWPWRQPRKQQANFH*LPNRN*QTIPDASDRC SGQQH*CQVAAFKFPCYWLQSNHHSQKWTRTNK N*NCRSRSNRNDY*RLAAHSGVCG*CLCSESKR RESASGSDCSNQH*SP*RTGIH*CGCRFHQNCLG KPTGASFQVQGDLLEP*GWNP*AIPCT*W*RRH CRAARPQTGF*VHSQCGCLAR*YGEPAPDWNPV HSYSCTN*PEVHSGHTHKPERPVDTTQCSAHWIS SAGDPQGEDRTNERNQPCS*QLIRGCIRTYGGH QI*SECLCS*GHFDKQTSSGSCHHSGECQPTKK GSCDRCY*DHHHH*LENQD*DDHWLPS*CRSSQW PDSNPENHQARCQKLHHHRFTTRH*LQDLPVHL E*QCSELPCGHRRLHCH*CTIQPAFPGHHTQFL AGIMAAATCQDYRLHHQV*EAWVSSQRSGPSAPP WCHRGYYYWPGTGNRIYNLCHCPEE*SEERAPD WKEKDRRASPTGNPSTPQSSWTRDLGCSFHSSK DPFRHPPWV*HWKWYSASWHFWSATQCWATNDL* GTWF*ADHTAHNGHPHKA*AKTIPAECRTRSSL SDNHLMGPIPGHF*VHHFMSSCWH**RTLTVQG SWNFYQCHSDRPHQRCHLQRHSGGTERPAEA*GS GRGCYRGQLCQRRLEPTYG*LVL*PLHSFPLCR WR*VGTNV*IRL*TVVPVLRLWKWSFQM*FI*M VP*QWCELQDWREVGPSGRKWPDDELHMSWERKR RIQV*PS*GNVL**WEDIPRRRTVAEGISRCHL LLHMLWRPAGLAL*QLPQTWG*TQSRRHYWPVL QPVFSEIPSENKH*C*LPN*VLHAFRCTG*QRRF PRVNHLSNPEEQACLSAKIHLNWSDVSRPSLEF FFLS*ALCSGGSSPASAQLTASPSITLGVS*GF SHK*GLHIACSASKYSIPLSILNEVILRFGLGSI GKHMQPTKMQMF*NDMTKILSRKVTQTLLLSLK CLARNTVGTSMILLL*YFKYPQYSLFPNDPSNC LEISFSYLLFINFSQYFYTEKIVLKTLSMQLIRG IWYNYGG*LFFILYVPKLYYCGKTTVLIKDLHS TT*SSSI*YKTPSGEIIPVNILFQFSKHLKIYD VQV*LLISVQDFLNQLLQKLIGFCHFISSLMEIA LHFLL**I*VDPNIY*NC*FTVQKYNRNNL*LL FSNHCNSSLLPSTFPSLNKPLFKEIACKGNKND *DIKSI*IV*YGGVLS*GNPMV**PPSNMSYFGS ADTFGMA**TPS*TVCLLLICKGHFFFNLTLRK QTLGLNLTFRPGAVAHTSNPSTLGG*GRWIT*G QEFETSLANMVKPCLY*KYKCYQAWQWASLVPAT QKAEVGEWLEPRRRRLQ*AEIVPLNSSMGDRAR LHPKKHK*INVTFRIVNSGNGNNY**KISSS** TRHAKHFLNILMYNERRYFNKHLAKNIKTKYRKI LPHVHLKKKNTNSHLN*FTVYKML*HLAISFRG GFRA*FYRA*SSDEVSLAKYRPSPWTSIYY*SL SFISPIVTKRAALYVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVD ITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIP IFEDFVDSSVGYYTVTGLEPGIDYDISVITLIN GGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVT WAPPPSIDLTNFLVRYSPVKNEEDVAELSISPS DNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRG RQKTGLDSPTGIDFSDITANSFTVHWIAPRATIT GYRIRHHPEHFSGRPREDRVPHSRNSITLTNLT PGTEYVVSIVALNGREESPLLIGQQSTVSDVPR DLEVVAATPTSLLISWDAPAVTVRYYRITYGETG GNSPVQEFTVPGSKSTATISGLKPGVDYTITVY AVTGRGDSPASSKPISINYRTEIDKPSQMQVTD VQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTK TKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSG ESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAW ESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDT AELQGLRPGSEYTVSVVALHDDMESQPLIGTQS TAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGY RVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVAT KYEVSVYALKDTLTSRPAQGVVTTLENVSPPRR ARVTDATETTITISWRTKTETITGFQVDAVPAN GQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTL NDNARSSPVVIDASTAIDAPSNLRFLATTPNSL LVSWQPPRARITGYIIKYEKPGSPPREVVPRPR PGVTEATITGLEPGTEYTIYVIALKNNQKSEPLI GRKKTDELPQLVTLPHPNLHGPEILDVPSTVQK TPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIF EEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALS QTTISWAPFQDTSEYIISCHPVGTDEEPLQFRV PGTSTSATLTGLTRGATYNVIVEALKDQQRHKV REEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAV GDEWERMSESGFKLLCQCLGFGSGHFRCDSSRW CHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGK GEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAIC SCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSY NQYSQRYHQRTNTNVNCPIECFMPLDVQADRED SRE*IIFPIQRNKHVSLPRSI*TGVMLADPA*SS SFFLKPFALEEVLQLQLNSQLLQASPWEFPEGF LINEGCTLPVLLRSIQYRSVF*MK*F*DLVWDQ *ESICSQPRCKCFEMI*PKF*VGKSPKHFCFHLS VWPAIL*EQA*SCYCDILNIHSTHFFQMILVIA *KYLSLTCYLSIFPSIFIRKKLY*KHLVCS**E EFGIIMVGDYFLYCMCQSFTTVERQLF**KIYIP QLEVHLFDIRHLRGK*FL*IFFFNSANI*KSMM CKSNC*FQYKIF*ISCYKN*LVFVTSSLH*WR* LYTFCFNRFKWTPIFIKIASLPFRSIIEIIFSCS FLTIVILPFFPPPFLH*INLCSKRLPAREIKMT KILKVFE*YNMEEFYLRETPWYDNPHLTCLTLG QLTLLAWLDKLPAKQFVYS*SVKVISFSI*H*GN KLWD*I*HSGQAQWLTPLIPALWEAEAGGSPEV RSLRPAWPTW*NLVSIKNTNVTRHGSGRLWSRL LRRLR*ENGLNPEGGGCSELRLCH*TPAWGTEQD SIPKNINK*M*HSE**IVEMEIIISKKLVVHNK QDMQSTF*IY*CTMKEGISINTWQRILKLNTER YFLMFI*KKKILTPI*IDLQFIKCFDI*LSHLGE VLELNFTGHEAQMRFP*RNTDPAPGLLFITDL* ALSAQ*SPRELLCMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQRCPNSLT*ALL I*PIQASA*GGPR*TLPPLLGTASQ*LRQEKVSL FLKILWTPQ*DTTQSQGWSRALTMISALSLSLM AARVPLLH*HNKRLFLLPLTCDSPTLVQTPCVS PGLHPHPLI*PTSWCVTHL*KMRKMLQSCQFLLQ TMQWS*QISCLVQNM**VSPVSTNNMRAHLLEE DRKQVLIPQLALTFLILLPTLLLCTGLLLEPPS LATGSAIIPSTSVGDLEKIGCPTLGIPSPSPTSL QAQSMWSASLLLMAERKVPY*LANNQQFLMFRG TWKLLLRPPPAY*SAGMLLLSQ*DITGSLTERQ EEIALSRSSLCLGASLQLPSAALNLELIIPSLCM LSLAVETAPQAASQFPLITEQKLTNHPRCK*PM FRTTALVSSGCLQVPLLLVTE*PPLPKMDQDQQ KLKLQVQIKQK*LLKACSPQWSMWLVSMLRIQAE RVSLWFRLQ*PTLIALKDWHSLMWMSIPSKLLG KAHRGKFPGTG*PTRALRMESMSYSLHLMVKKT LQSCKASDRVLSTQSVWLPCTMIWRASP*LEPSP QLFLHQLT*SSLRSHPQA*APSGHHPMFSSLDI ECG*PPRRRPDQ*KKSTLLLTAHPWLYQDLWWP PNMK*VSMLLRTL*QADQLRELSPLWRMSAHQEG LV*QMLLRPPSPLAGEPRLRRSLASKLMPFQPM ARLQSREPSSQMSEATPSQVYNQALTTRSTCTP *MTMLGAPLWSSTPPLPLMHHPTCVSWPPHPIPC WYHGSRHVPGLPATSSSMRSLGLLPEKWSLGPA LVSQRLLLLAWNREPNIQFMSLP*RIIRRASP* LEGKRQTSFPNW*PFHTPIFMDQRSWMFLPQFKR PLSSPTLGMTLEMVFSFLALLVSNPVLGNK*SL RNMVLGGPHRPQRPPP*GIGQDHTRRM*DKKLS LRQPSHGPHSRTLLSTSFHVILLALMKNPYSSGF LELLPVPL*QASPEVPPTTS*WRH*KTSRGIRF GKRLLPWATLSTKA*TNLRMTRALTPTQFPIMP LEMSGNECLNQALNCCASA*ALEVVISDVIHLDG AMTMV*TTRLERSGTVREKMAR**AAHVLGTEK ENSSVTLMRQRVMMMGRHTT*ENSGRRNISVPF APAHALEASGAGAVTTAADLGVNPVPKALLASPT TSILRDTIREQTLMLIAQLSASCL*MYRLTEKI PESKSSFQSRGTSMSLCQDPSKLE*C*QTQLRV LLSFLSPLLWRKFSSFSSTHSFSKHHPGSFLRVF S*MRAAHCLFCFEVFNTAQYFK*SDSKIWFGIN RKAYAANQDANVLK*YDQNFK*ESHPNTSAFT* VSGPQYCRNKHDLVTVIF*ISTVLTFSK*S**LP RNIFLLPVIYQFFPVFLYGKNCIENT*YAVDKR NLV*LWWVIIFYTVCAKALLLWKDNCFNKRFTF HNLKFIYLI*DTFGGNNSCEYSFSIQQTFENL*C ASLIVDFSTRFSKSVATKTDWFLSLHLFTNGDS FTLSALIDLSGPQYLLKLLVYRSEV**K*SLVA LF*PL*FFPSSLHLSFIE*TSVQRDCLQGK*K*L RY*KYLNSIIWRSFILGKPHGMITPI*HVLLWV S*HFWHGLINSQLNSLFTPDL*RSFLFQFNIEE TNFGIKFDIQARRSGSHL*SQHFGRLRQVDHLRS GV*DQPGQHGETLSLLKIQMLPGMAVGVFGPGY SEG*GRRMA*TQKAEVAVS*DCAIELQHGGQSK TPSQKT*INKCDIQNSK*WKWK*LLVKN**FIIN KTCKALFKYTNVQ*KKVFQ*TPGKEY*N*IQKD TSSCSFEKKKY*LPFELIYSL*NALTFSYLI*G RF*SLILQGMKLR*GFLSEIQTQPLDFYLLLIFK LYQPNSHQESCSVCFLKITESTK*R*Y*TTV

Sequence FN1.aAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGAGGTGCCCCAACTCACTGACCTAAGCTTTGTTGA TATAACCGATTCAAGCATCGGCCTGAGGTGGACCCCGCTAAACTCTTCCACCATTATTGGGTACCGCATCACAGTAGTTGCGGCAGGAGAAGGTATCCCT ATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTCACAGGGCTGGAGCCGGGCATTGACTATGATATCAGCGTTATCACTCTCATTAATG GCGGCGAGAGTGCCCCTACTACACTGACACAACAAACGGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAACATTGGTCCAGACACCATGCGTGTCAC CTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGATGTTGCAGAGTTGTCAATTTCTCCTTCA GACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAACAACATGAGAGCACACCTCTTAGAGGAA GACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCACTGGATTGCTCCTCGAGCCACCATCAC TGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGGAATTCCATCACCCTCACCAACCTCACT CCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAACAATCAACAGTTTCTGATGTTCCGAGGG ACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATATTACAGGATCACTTACGGAGAGACAGG AGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCTGGAGTTGATTATACCATCACTGTGTAT GCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACAAACCATCCCAGATGCAAGTGACCGATG TTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCACTCCCAAAAATGGACCAGGACCAACAAA AACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTTAGTGTCTATGCTCAGAATCCAAGCGGA GAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCTTGGG AAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATTCCCTGCACCTGATGGTGAAGAAGACAC TGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAGAGCCAGCCCCTGATTGGAACCCAGTCC ACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATC GAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCAC CAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGG GCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATG GCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTT GAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTG CTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCC CTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGAT TGGAAGGAAAAAGACAGACGAGCTTCCCCAACTGGTAACCCTTCCACACCCCAATCTTCATGGACCAGAGATCTTGGATGTTCCTTCCACAGTTCAAAAG ACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTG AGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGTGAGGAAATCCAAAT TGGTCACATCCCCAGGGAAGATGTAGACTATCACCTGTACCCACACGGTCCGGGACTCAATCCAAATGCCTCTACAGGACAAGAAGCTCTCTCTCAGACA ACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAA CTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGA GGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGAT GAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATG ACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATT CAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGC ACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGT ATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGA GTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTC TTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAA ATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAG CATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGG CCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAAT ATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGG TATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTT GAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGT CTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACAC TTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTA ACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATAT TAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGAC ACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTT TGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTT TGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAG GCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATC CCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACA TGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCT CATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTA GAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTAT CAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.40 (forward primer)
FN1.u.r.45 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPRGAPTH*PKLC* YNRFKHRPEVDPAKLFHHYWVPHHSSCGRRRYP YF*RFCGLLSRILHSHRAGAGH*L*YQRYHSH*W RRECPYYTDTTNGCSSSH*PAIHQHWSRHHACH LGSTPIH*FNQLPGALLTCEK*GRCCRVVNFSF RQCSGLNKSPAWYRICSECLQCLRTT*EHTS*RK TENRS*FPNWH*LF*YYCQLFYCALDCSSSHHH WLQDPPSSRALQWETSRRSGAPLSEFHHPHQPH SRHRVCGQHRCS*WQRGKSLIDWPTINSF*CSEG PGSCCCDPHQPTDQLGCSCCHSEILQDHLRRDR RK*PCPGVHCAWEQVYSYHQRP*TWS*LYHHCV CCHWPWRQPRKQQANFH*LPNRN*QTIPDASDRC SGQQH*CQVAAFKFPCYWLQSNHHSQKWTRTNK N*NCRSRSNRNDY*RLAAHSGVCG*CLCSESKR RESASGSDCSNQH*SP*RTGIH*CGCRFHQNCLG KPTGASFQVQGDLLEP*GWNP*AIPCT*W*RRH CRAARPQTGF*VHSQCGCLAR*YGEPAPDWNPV HSYSCTN*PEVHSGHTHKPERPVDTTQCSAHWIS SAGDPQGEDRTNERNQPCS*QLIRGCIRTYGGH QI*SECLCS*GHFDKQTSSGSCHHSGECQPTKK GSCDRCY*DHHHH*LENQD*DDHWLPS*CRSSQW PDSNPENHQARCQKLHHHRFTTRH*LQDLPVHL E*QCSELPCGHRRLHCH*CTIQPAFPGHHTQFL AGIMAAATCQDYRLHHQV*EAWVSSQRSGPSAPP WCHRGYYYWPGTGNRIYNLCHCPEE*SEERAPD WKEKDRRASPTGNPSTPQSSWTRDLGCSFHSSK DPFRHPPWV*HWKWYSASWHFWSATQCWATNDL* GTWF*ADHTAHNGHPHKA*AKTIPAECR*GNPN WSHPQGRCRLSPVPTRSGTQSKCLYRTRSSLSD NHLMGPIPGHF*VHHFMSSCWH**RTLTVQGSWN FYQCHSDRPHQRCHLQRHSGGTERPAEA*GSGR GCYRGQLCQRRLEPTYG*LVL*PLHSFPLCRWR *VGTNV*IRL*TVVPVLRLWKWSFQM*FI*MVP* QWCELQDWREVGPSGRKWPDDELHMSWERKRRI QV*PS*GNVL**WEDIPRRRTVAEGISRCHLLL HMLWRPAGLAL*QLPQTWG*TQSRRHYWPVLQPV FSEIPSENKH*C*LPN*VLHAFRCTG*QRRFPR VNHLSNPEEQACLSAKIHLNWSDVSRPSLEFFF LS*ALCSGGSSPASAQLTASPSITLGVS*GFSHK *GLHIACSASKYSIPLSILNEVILRFGLGSIGK HMQPTKMQMF*NDMTKILSRKVTQTLLLSLKCL ARNTVGTSMILLL*YFKYPQYSLFPNDPSNCLEI SFSYLLFINFSQYFYTEKIVLKTLSMQLIRGIW YNYGG*LFFILYVPKLYYCGKTTVLIKDLHSTT *SSSI*YKTPSGEIIPVNILFQFSKHLKIYDVQV *LLISVQDFLNQLLQKLIGFCHFISSLMEIALH FLL**I*VDPNIY*NC*FTVQKYNRNNL*LLFS NHCNSSLLPSTFPSLNKPLFKEIACKGNKND*DI KSI*IV*YGGVLS*GNPMV**PPSNMSYFGSAD TFGMA**TPS*TVCLLLICKGHFFFNLTLRKQT LGLNLTFRPGAVAHTSNPSTLGG*GRWIT*GQEF ETSLANMVKPCLY*KYKCYQAWQWASLVPATQK AEVGEWLEPRRRRLQ*AEIVPLNSSMGDRARLH PKKHK*INVTFRIVNSGNGNNY**KISSS**TRH AKHFLNILMYNERRYFNKHLAKNIKTKYRKILP HVHLKKKNTNSHLN*FTVYKML*HLAISFRGGF RA*FYRA*SSDEVSLAKYRPSPWTSIYY*SLSFI SPIVTKRAALYVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVD ITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIP IFEDFVDSSVGYYTVTGLEPGIDYDISVITLIN GGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVT WAPPPSIDLTNFLVRYSPVKNEEDVAELSISPS DNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRG RQKTGLDSPTGIDFSDITANSFTVHWIAPRATIT GYRIRHHPEHFSGRPREDRVPHSRNSITLTNLT PGTEYVVSIVALNGREESPLLIGQQSTVSDVPR DLEVVAATPTSLLISWDAPAVTVRYYRITYGETG GNSPVQEFTVPGSKSTATISGLKPGVDYTITVY AVTGRGDSPASSKPISINYRTEIDKPSQMQVTD VQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTK TKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSG ESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAW ESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDT AELQGLRPGSEYTVSVVALHDDMESQPLIGTQS TAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGY RVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVAT KYEVSVYALKDTLTSRPAQGVVTTLENVSPPRR ARVTDATETTITISWRTKTETITGFQVDAVPAN GQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTL NDNARSSPVVIDASTAIDAPSNLRFLATTPNSL LVSWQPPRARITGYIIKYEKPGSPPREVVPRPR PGVTEATITGLEPGTEYTIYVIALKNNQKSEPLI GRKKTDELPQLVTLPHPNLHGPEILDVPSTVQK TPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIF EEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQI GHIPREDVDYHLYPHGPGLNPNASTGQEALSQT TISWAPFQDTSEYIISCHPVGTDEEPLQFRVPG TSTSATLTGLTRGATYNVIVEALKDQQRHKVREE VVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGD EWERMSESGFKLLCQCLGFGSGHFRCDSSRWCH DNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEF KCDPHEATCYDDGKTYHVGEQWQKEYLGAICSC TCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQ YSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE *IIFPIQRNKHVSLPRSI*TGVMLADPA*SSSF FLKPFALEEVLQLQLNSQLLQASPWEFPEGFLI NEGCTLPVLLRSIQYRSVF*MK*F*DLVWDQ*ES ICSQPRCKCFEMI*PKF*VGKSPKHFCFHLSVW PAIL*EQA*SCYCDILNIHSTHFFQMILVIA*K YLSLTCYLSIFPSIFIRKKLY*KHLVCS**EEFG IIMVGDYFLYCMCQSFTTVERQLF**KIYIPQL EVHLFDIRHLRGK*FL*IFFFNSANI*KSMMCK SNC*FQYKIF*ISCYKN*LVFVTSSLH*WR*LYT FCFNRFKWTPIFIKIASLPFRSIIEIIFSCSFL TIVILPFFPPPFLH*INLCSKRLPAREIKMTKI LKVFE*YNMEEFYLRETPWYDNPHLTCLTLGQLT LLAWLDKLPAKQFVYS*SVKVISFSI*H*GNKL WD*I*HSGQAQWLTPLIPALWEAEAGGSPEVRS LRPAWPTW*NLVSIKNTNVTRHGSGRLWSRLLRR LR*ENGLNPEGGGCSELRLCH*TPAWGTEQDSI PKNINK*M*HSE**IVEMEIIISKKLVVHNKQD MQSTF*IY*CTMKEGISINTWQRILKLNTERYFL MFI*KKKILTPI*IDLQFIKCFDI*LSHLGEVL ELNFTGHEAQMRFP*RNTDPAPGLLFITDL*AL SAQ*SPRELLCMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQRCPNSLT*ALL I*PIQASA*GGPR*TLPPLLGTASQ*LRQEKVSL FLKILWTPQ*DTTQSQGWSRALTMISALSLSLM AARVPLLH*HNKRLFLLPLTCDSPTLVQTPCVS PGLHPHPLI*PTSWCVTHL*KMRKMLQSCQFLLQ TMQWS*QISCLVQNM**VSPVSTNNMRAHLLEE DRKQVLIPQLALTFLILLPTLLLCTGLLLEPPS LATGSAIIPSTSVGDLEKIGCPTLGIPSPSPTSL QAQSMWSASLLLMAERKVPY*LANNQQFLMFRG TWKLLLRPPPAY*SAGMLLLSQ*DITGSLTERQ EEIALSRSSLCLGASLQLPSAALNLELIIPSLCM LSLAVETAPQAASQFPLITEQKLTNHPRCK*PM FRTTALVSSGCLQVPLLLVTE*PPLPKMDQDQQ KLKLQVQIKQK*LLKACSPQWSMWLVSMLRIQAE RVSLWFRLQ*PTLIALKDWHSLMWMSIPSKLLG KAHRGKFPGTG*PTRALRMESMSYSLHLMVKKT LQSCKASDRVLSTQSVWLPCTMIWRASP*LEPSP QLFLHQLT*SSLRSHPQA*APSGHHPMFSSLDI ECG*PPRRRPDQ*KKSTLLLTAHPWLYQDLWWP PNMK*VSMLLRTL*QADQLRELSPLWRMSAHQEG LV*QMLLRPPSPLAGEPRLRRSLASKLMPFQPM ARLQSREPSSQMSEATPSQVYNQALTTRSTCTP *MTMLGAPLWSSTPPLPLMHHPTCVSWPPHPIPC WYHGSRHVPGLPATSSSMRSLGLLPEKWSLGPA LVSQRLLLLAWNREPNIQFMSLP*RIIRRASP* LEGKRQTSFPNW*PFHTPIFMDQRSWMFLPQFKR PLSSPTLGMTLEMVFSFLALLVSNPVLGNK*SL RNMVLGGPHRPQRPPP*GIGQDHTRRM*VRKSK LVTSPGKM*TITCTHTVRDSIQMPLQDKKLSLRQ PSHGPHSRTLLSTSFHVILLALMKNPYSSGFLE LLPVPL*QASPEVPPTTS*WRH*KTSRGIRFGK RLLPWATLSTKA*TNLRMTRALTPTQFPIMPLEM SGNECLNQALNCCASA*ALEVVISDVIHLDGAM TMV*TTRLERSGTVREKMAR**AAHVLGTEKEN SSVTLMRQRVMMMGRHTT*ENSGRRNISVPFAPA HALEASGAGAVTTAADLGVNPVPKALLASPTTS ILRDTIREQTLMLIAQLSASCL*MYRLTEKIPE SKSSFQSRGTSMSLCQDPSKLE*C*QTQLRVLLS FLSPLLWRKFSSFSSTHSFSKHHPGSFLRVFS* MRAAHCLFCFEVFNTAQYFK*SDSKIWFGINRK AYAANQDANVLK*YDQNFK*ESHPNTSAFT*VSG PQYCRNKHDLVTVIF*ISTVLTFSK*S**LPRN IFLLPVIYQFFPVFLYGKNCIENT*YAVDKRNL V*LWWVIIFYTVCAKALLLWKDNCFNKRFTFHNL KFIYLI*DTFGGNNSCEYSFSIQQTFENL*CAS LIVDFSTRFSKSVATKTDWFLSLHLFTNGDSFT LSALIDLSGPQYLLKLLVYRSEV**K*SLVALF* PL*FFPSSLHLSFIE*TSVQRDCLQGK*K*LRY *KYLNSIIWRSFILGKPHGMITPI*HVLLWVS* HFWHGLINSQLNSLFTPDL*RSFLFQFNIEETNF GIKFDIQARRSGSHL*SQHFGRLRQVDHLRSGV *DQPGQHGETLSLLKIQMLPGMAVGVFGPGYSE G*GRRMA*TQKAEVAVS*DCAIELQHGGQSKTPS QKT*INKCDIQNSK*WKWK*LLVKN**FIINKT CKALFKYTNVQ*KKVFQ*TPGKEY*N*IQKDTS SCSFEKKKY*LPFELIYSL*NALTFSYLI*GRF* SLILQGMKLR*GFLSEIQTQPLDFYLLLIFKLY QPNSHQESCSVCFLKITESTK*R*Y*TTV