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Sequences

Sequence AFF3.fAug05

AAAGTGCCATTCAGAAAGCTCGCCAGCGAGAAGAGAGCTCCACGCTCAGCCTCAGAAGAAAGACGTTCAACGACTTACTGGGGAGAGAAAGAAAAGGAAC GGGAGCTGAGAGAAAGTTTCACACATATGATTTAGACCTTACGATCAGCCATTGTTTACATCGGGAAGCATCAATCCGGAGTAACTGGCAAGCAGCTGAT CACCTGCCTTTCCAGACCGGAGACGGGCGGCAAGTTTGAAGTCGACACCCAGAGGCCAGTTTGTCCCCCTTCCCGCGCGCGGGCGGCCCGAGCCTCGGCG GCGGCGGTAGCGGCGGCGGCGACGCTGACACCTCCCACCATGGACAGCTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGAGTCACTGTGTGTCTATG AACCAGATAGAAATGCATTACGGAGGAAAGAACGAGAAAGAAGAAATCAAGAAACTCAACAGGATGATGGCACGTTTAATTCTAGTTACTCTCTCTTCAG TGAGCCCTACAAGACTAACAAGGGGGATGAACTCTCCAACCGGATCCAGAACACTTTAGGCAATTATGATGAAATGAAAGACTTTTTAACTGATAGATCC AATCAGAGTCATCTCGTTGGAGTTCCCAAACCTGGGGTTCCTCAGACTCCTGTGAACAAGATCGATGAACATTTTGTTGCAGATTCAAGAGCCCAGAACC AGCCCTCGTCTATCTGTAGCACTACAACTTCCACACCAGCAGCTGTCCCCGTGCAGCAGAGTAAGAGAGGCACTATGGG

AFF3.F2 (forward primer)
AFF3.R31 (reverse primer)
Single exon skipping

Frame 0

KVPFRKLASEKRAPRSASEERRSTTYWGEKEKE RELRESFTHMI*TLRSAIVYIGKHQSGVTGKQLI TCLSRPETGGKFEVDTQRPVCPPSRARAARASA AAVAAAATLTPPTMDSFDLALLQEWDLESLCVY EPDRNALRRKERERRNQETQQDDGTFNSSYSLFS EPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRS NQSHLVGVPKPGVPQTPVNKIDEHFVADSRAQN QPSSICSTTTSTPAAVPVQQSKRGTM

Frame 1

KCHSESSPARRELHAQPQKKDVQRLTGERKKRN GS*EKVSHI*FRPYDQPLFTSGSINPE*LASS* SPAFPDRRRAASLKSTPRGQFVPLPARGRPEPRR RR*RRRRR*HLPPWTAST*PCSRNGTSSHCVSM NQIEMHYGGKNEKEEIKKLNRMMARLILVTLSS VSPTRLTRGMNSPTGSRTL*AIMMK*KTF*LIDP IRVISLEFPNLGFLRLL*TRSMNILLQIQEPRT SPRLSVALQLPHQQLSPCSRVREALW

Frame 2

SAIQKARQREESSTLSLRRKTFNDLLGRERKGT GAERKFHTYDLDLTISHCLHREASIRSNWQAAD HLPFQTGDGRQV*SRHPEASLSPFPRAGGPSLG GGGSGGGDADTSHHGQLRLSPAPGMGPRVTVCL* TR*KCITEERTRKKKSRNSTG*WHV*F*LLSLQ *ALQD*QGG*TLQPDPEHFRQL**NERLFN**I QSESSRWSSQTWGSSDSCEQDR*TFCCRFKSPEP ALVYL*HYNFHTSSCPRAAE*ERHYG

Sequence AFF3.cAug05

GTGTGCCTGCGCGCAAGCCGGGGAGCGGCGCGCGGGCGGCCCGAGCCTCGGCGGCGGCGGTAGCGGCGGCGGCGACGCTGACACCTCCCACCATGGACAG CTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGAGTCACTGTGTGTCTATGAACCAGATAGAAATGCATTACGGAGGAAAGAACGAGAAAGAAGAAAT CAAGAAACTCAACAGGATGATGGCACGTTTAATTCTAGTTACTCTCTCTTCAGTGAGCCCTACAAGACTAACAAGGGGGATGAACTCTCCAACCGGATCC AGAACACTTTAGGCAATTATGATGAAATGAAAGACTTTTTAACTGATAGATCCAATCAGAGTCATCTCGTTGGAGTTCCCAAACCTGGGGTTCCTCAGAC TCCTGTGAACAAGATCGATGAACATTTTGTTGCAGATTCAAGAGCCCAGAACCAGCCCTCGTCTATCTGTAGCACTACAACTTCCACACCAGCAGCTGTC CCCGTGCAGCAGAGTAAGAGAGGCACTATGGGCTGGCAGAAGGCTGGGCACCCACCCTCTGACGGCCAACAGAGAGCAACACAACAGGGCTCTCTCAGGA CCTTGCTTGGAGATGGTGTTGGCAGACAGCAGCCTCGGGCCAAACAAGTGTGCAATGTGGAGGTGGGCCTTCAGACCCAGGAGAGGCCACCTGCCATGGC GGCCAAGCACAGCAGCAGCGGACACTGTGTTCAGAACTTTCCTCCATCCCTAGCTTCAAAACCCAGCCTGGTCCAGCAGAAACCGACCGCGTATGTGAGG CCAATGGACGGCCAAGATCAGGCCCCTGATGAGTCTCCTAAGCTGAAGTCGTCTTCGGAAACCAGCGTGCACTGCACATCATACAGGGGAGTCCCTGCCA GCAAGCCGGAGCCTGCCAGAGCCAAGGCCAAGCTCTCCAAGTTCAGCATCCCCAAGCAGGGGGAGGAGAGTAGATCTGGAGAAACCAACAGCTGTGTTGA AGAAATAATCCGGGTAAGATTATTCTTTAAACAGTCTTATCAATCTGTGGGACATAGACTCTAAATAGAAAGGAAACCTGCGGCTCCATGATAAAGGAGC CCATAGTCTGTGGTTTGACATTTACCTTTTCTGAGAGTAGTCACATAAGTTTTTGAAACAACACTTCTGTTAACAATCTAGGAGCTGCACCCCCTCTGGG CACCTCTGCACAGGCTGAGGTGACACCCTATTAAAGTGATCAGCTGGAAGACAGAAGAACAGAATTTAGATGAAAAATATGCTGGTGCTCACATGTGGAT GCCCCATTTATCACCCTGCTATTTAAAAGCATCCTTTAATAGTCTGTACATTTTTTTGAGTCAGGCAGGGGTTGCCTGGTTATTTTTCCTGTAAACTTAA TTGGAATCAGAAGCACTTGACAAAGAGGTAATGTTCAGGATCTGGAACTGTTATTTCTCTATTGTTTCACATGTTATTTTCCCTGTCACCCTCTGTGAGC CCGTCCTTTTCATCACCGTTGT

AFF3.F2 (forward primer)
AFF3.R31 (reverse primer)
Single exon skipping

Frame 0

VCLRASRGAARGRPEPRRRR*RRRRR*HLPPWT AST*PCSRNGTSSHCVSMNQIEMHYGGKNEKEEI KKLNRMMARLILVTLSSVSPTRLTRGMNSPTGS RTL*AIMMK*KTF*LIDPIRVISLEFPNLGFLR LL*TRSMNILLQIQEPRTSPRLSVALQLPHQQLS PCSRVREALWAGRRLGTHPLTANREQHNRALSG PCLEMVLADSSLGPNKCAMWRWAFRPRRGHLPW RPSTAAADTVFRTFLHP*LQNPAWSSRNRPRM*G QWTAKIRPLMSLLS*SRLRKPACTAHHTGESLP ASRSLPEPRPSSPSSASPSRGRRVDLEKPTAVL KK*SG*DYSLNSLINLWDIDSK*KGNLRLHDKGA HSLWFDIYLF*E*SHKFLKQHFC*QSRSCTPSG HLCTG*GDTLLK*SAGRQKNRI*MKNMLVLTCG CPIYHPAI*KHPLIVCTFF*VRQGLPGYFSCKLN WNQKHLTKR*CSGSGTVISLLFHMLFSLSPSVS PSFSSPL

Frame 1

CACAQAGERRAGGPSLGGGGSGGGDADTSHHGQ LRLSPAPGMGPRVTVCL*TR*KCITEERTRKKK SRNSTG*WHV*F*LLSLQ*ALQD*QGG*TLQPDP EHFRQL**NERLFN**IQSESSRWSSQTWGSSD SCEQDR*TFCCRFKSPEPALVYL*HYNFHTSSC PRAAE*ERHYGLAEGWAPTL*RPTESNTTGLSQD LAWRWCWQTAASGQTSVQCGGGPSDPGEATCHG GQAQQQRTLCSELSSIPSFKTQPGPAETDRVCE ANGRPRSGP**VS*AEVVFGNQRALHIIQGSPCQ QAGACQSQGQALQVQHPQAGGGE*IWRNQQLC* RNNPGKIIL*TVLSICGT*TLNRKETCGSMIKE PIVCGLTFTFSESSHISF*NNTSVNNLGAAPPLG TSAQAEVTPY*SDQLEDRRTEFR*KICWCSHVD APFITLLFKSIL**SVHFFESGRGCLVIFPVNL IGIRST*QRGNVQDLELLFLYCFTCYFPCHPL*A RPFHHRC

Frame 2

VPARKPGSGARAARASAAAVAAAATLTPPTMDS FDLALLQEWDLESLCVYEPDRNALRRKERERRN QETQQDDGTFNSSYSLFSEPYKTNKGDELSNRI QNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQT PVNKIDEHFVADSRAQNQPSSICSTTTSTPAAV PVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLR TLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMA AKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVR PMDGQDQAPDESPKLKSSSETSVHCTSYRGVPA SKPEPARAKAKLSKFSIPKQGEESRSGETNSCVE EIIRVRLFFKQSYQSVGHRL*IERKPAAP**RS P*SVV*HLPFLRVVT*VFETTLLLTI*ELHPLW APLHRLR*HPIKVISWKTEEQNLDEKYAGAHMWM PHLSPCYLKASFNSLYIFLSQAGVAWLFFL*T* LESEALDKEVMFRIWNCYFSIVSHVIFPVTLCE PVLFITV

Sequence AFF3.aAug05

AAAGTGCCATTCAGAAAGCTCGCCAGCGAGAAGAGAGCTCCACGCTCAGCCTCAGAAGAAAGACGTTCAACGACTTACTGGGGAGAGAAAGAAAAGGAAC GGGAGCTGAGAGCTGATCACCTGCCTTTCCAGACCGGAGACGGGCGGCAAGTTTGAAGTCGACACCCAGAGGCCAGTTTGTCCCCCTTCCCGCGCGCGGG CGGCCCGAGCCTCGGCGGCGGCGGTAGCGGCGGCGGCGACGCTGACACCTCCCACCATGGACAGCTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGA GTCACTGTGTGTCTATGAACCAGATAGAAATGCATTACGGAGGAAAGAACGAGAAAGAAGAAATCAAGAAACTCAACAGGATGATGGCACGTTTAATTCT AGTTACTCTCTCTTCAGTGAGCCCTACAAGACTAACAAGGGGGATGAACTCTCCAACCGGATCCAGAACACTTTAGGCAATTATGATGAAATGAAAGACT TTTTAACTGATAGATCCAATCAGAGTCATCTCGTTGGAGTTCCCAAACCTGGGGTTCCTCAGACTCCTGTGAACAAGATCGATGAACATTTTGTTGCAGA TTCAAGAGCCCAGAACCAGCCCTCGTCTATCTGTAGCACTACAACTTCCACACCAGCAGCTGTCCCCGTGCAGCAGAGTAAGAGAGGCACTATGGGCTGG CAGAAGGCTGGGCACCCACCCTCTGACGGCCAACAGAGAGCAACACAACAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTGTTGGCAGACAGCAGCCTC GGGCCAAACAAGTGTGCAATGTGGAGGTGGGCCTTCAGACCCAGGAGAGGCCACCTGCCATGGCGGCCAAGCACAGCAGCAGCGGACACTGTGTTCAGAA CTTTCCTCCATCCCTAGCTTCAAAACCCAGCCTGGTCCAGCAGAAACCGACCGCGTATGTGAGGCCAATGGACGGCCAAGATCAGGCCCCTGATGAGTCT CCTAAGCTGAAGTCGTCTTCGGAAACCAGCGTGCACTGCACATCATACAGGGGAGTCCCTGCCAGCAAGCCGGAGCCTGCCAGAGCCAAGGCCAAGCTCT CCAAGTTCAGCATCCCCAAGCAGGGGGAGGAGAGTAGATCTGGAGAAACCAACAGCTGTGTTGAAGAAATAATCCGGGAGATGACCTGGCTTCCACCACT TTCTGCTATTCAAGCACCTGGCAAAGTGGAACCAACCAAATTTCCATTTCCAAATAAGGACTCTCAGCTTGTATCCTCTGGACACAATAATCCAAAGAAA GGTGATGCAGAGCCAGAGAGTCCAGACAGTGGCACATCGAATACATCAATGCTGGAAGATGACCTTAAGCTAAGCAGTGATGAAGAGGAGAATGAACAGC AGGCAGCTCAGAGAACGGCTCTCCGCGCTCTCTCTGACAGCGCCGTGGTCCAGCAGCCCAACTGCAGAACCTCGGTGCCTTCCAGCAAGGGCAGCAGCAG CAGCAGCAGCAGCGGCAGCAGCAGCTCCTCCAGCGACTCAGAGAGCAGCTCCGGATCTGACTCGGAGACCGAGAGCAGCTCCAGCGAGAGTGAGGGCAGC AAGCCCCCCCACTTCTCCAGCCCCGAGGCTGAACCGGCATCCTCTAACAAGTGGCAGCTGGATAAATGGCTAAACAAAGTTAATCCCCACAAGCCTCCTA TTCTGATCCAAAATGAAAGCCACGGGTCAGAGAGCAATCAGTACTACAACCCGGTGAAAGAGGACGTCCAGGACTGTGGGAAAGTCCCCGACGTTTGCCA GCCCAGCCTGAGAGAGAAGGAGATCAAGAGCACTTGCAAGGAGGAGCAAAGGCCAAGGACAGCCAACAAGGCCCCTGGGAGTAAAGGCGTGAAGCAGAAG TCCCCGCCCGCGGCCGTGGCCGTGGCGGTGAGCGCAGCCGCCCCGCCACCCGCAGTGCCCTGTGCGCCCGCGGAGAACGCGCCCGCGCCTGCCCGGAGGT CCGCGGGCAAGAAGCCCACCAGGCGCACCGAGAGGACCTCAGCCGGGGACGGCGCCAACTGCCACCGGCCCGAGGAGCCCGCGGCCGCGGACGCGCTGGG GACGAGCGTGGTGGTCCCCCCGGAGCCCACCAAAACCAGGCCCTGTGGCAACAACAGAGCGAGCCACCGCAAGGAGCTGCGCTCCTCCGTGACCTGCGAG AAGCGCCGCACGCGGGGGCTAAGCAGGATCGTCCCCAAATCCAAGGAGTTCATTGAGACAGAGTCGTCATCTTCATCCTCCTCCTCGGACTCCGACCTGG AGTCCGAGCAGGAGGAGTACCCTCTGTCCAAAGCACAGACCGTGGCTGCCTCTGCCTCCTCCGGGAATGATCAGAGGCTGAAGGAGGCCGCTGCCAACGG GGGCAGTGGTCCTAGGGCCCCTGTAGGCTCCATCAACGCCAGGACCACCAGTGACATCGCCAAGGAGCTGGAGGAGCAGTTCTACACACTGGTCCCCTTT GGCCGGAACGAACTTCTCTCCCCTCTAAAGGACAGTGATGAGATCAGGTCTCTCTGGGTCAAAATCGACCTGACCCTCCTGTCCAGGATCCCAGAACACC TGCCCCAGGAGCCAGGGGTATTGAGCGCCCCTGCCACCAAGGACTCTGAGAGCGCACCGCCCAGCCACACCTCGGACACACCTGCAGAAAAGGCTTTGCC AAAATCCAAGAGGAAACGCAAGTGTGACAACGAAGACGACTACAGGGAGATCAAGAAGTCCCAGGGAGAGAAAGACAGCTCTTCAAGACTGGCCACCTCC ACCAGTAATACTTTGTCTGCAAACCACTGCAACATGAACATCAACAGTGTGGCAATACCAATAAATAAAAATGAAAAAATGCTTCGGTCGCCCATCTCAC CCCTCTCTGATGCATCTAAACACAAATACACCAGCGAGGACTTAACTTCTTCCAGCCGACCTAATGGCAACAGTTTGTTTACTTCAGCCTCTTCCAGCAA AAAGCCTAAGGCCGACAGCCAGCTGCAGCCTCACGGCGGAGACCTCACGAAAGCAGCTCACAACAATTCTGAAAACATTCCCCTCCACAAGTCACGGCCG CAGACGAAGCCGTGGTCTCCAGGCTCCAACGGCCACAGGGACTGCAAGAGGCAGAAACTTGTCTTCGATGATATGCCTCGCAGTGCCGATTATTTTATGC AAGAAGCTAAACGAATGAAGCATAAAGCAGATGCAATGGTGGAAAAGTTTGGAAAGGCTTTGAACTATGCTGAAGCAGCATTGTCGTTTATCGAGTGTGG AAATGCAATGGAACAAGGCCCCATGGAATCCAAATCTCCTTATACGATGTATTCAGAAACAGTAGAGCTCATCAGGTATGCTATGAGACTAAAAACCCAC TCAGGCCCCAATGCCACACCAGAAGACAAACAACTGGCTGCATTATGTTACCGATGCCTGGCCCTCCTGTACTGGCGGATGTTTCGACTCAAAAGGGACC ACGCTGTAAAGTATTCAAAAGCACTAATCGACTATTTCAAGAACTCATCTAAAGCCGCCCAAGCCCCATCTCCGTGGGGGGCCAGTGGAAAGAGCACTGG AACCCCATCCCCCATGTCTCCCAACCCCTCTCCCGCCAGCTCCGTGGGGTCTCAGGGCAGCCTCTCCAACGCCAGCGCCCTGTCCCCGTCGACCATCGTC AGCATCCCACAGCGCATCCACCAGATGGCGGCCAACCACGTCAGCATCACCAACAGCATCCTGCACAGCTACGACTACTGGGAGATGGCCGACAACCTGG CCAAGGAAAACCGAGAATTCTTCAACGACCTGGATCTGCTCATGGGGCCGGTCACCCTGCACAGCAGCATGGAGCACCTGGTCCAGTACTCCCAACAGGG CCTGCACTGGCTGCGGAACAGCGCCCACCTGTCATAGGGACCTCACCCTGGGGCCAGAGTGGGCTCTGGTCTCCACAGATGGCTCAACGTTTTTGGACAC TGTGCTACTGAAACTCCCAGCCACAGCATTTATCAGACTGCGGTGAACATTTCCTCAGCATTGAACAATGATCTTTTGCAGGGCATGTGTGTTTGTATGT GTGGGTATATAAGAAGCCGCCTGTGTATGTGTGTAAGATACACAGCTCTTCAGAACACACCTTCTTTGTCTAGTTTCCATAATCCCAGGCTAGACAAAGT GATCAGAGGTTTCTGACTAGAGGAAGTACACACACACACACACACACACACACACACCGTACACTCACACACAATCACAGACACACACTTTCTATTCCAT TCCAGTGCTAAACCGTGACTTCTTAGAAAATTCAACCAAAATCTCATGGGTTAGTTAACCAGGTGCAATACAGCACACCAAAAGCTTGACTGCCAGTGAA GATGGACCTGCTCTTATACTGTTGATTACTTTCAGTATTAATATACTATTCCCCAATTATTTTTTGATTGGTATGTATTAATGGGTAATTGAATAATGAA AATAAGCCAGGTATTTTTGTGCCGGAAGTTTACTAGATTGCATGTTACCAAATACCCTGCTCTTCCTCTAATTCCCTTCCCCCATCTGACTCTATTATCA ACTAAATGCATTCCAGGCAAACAGATGGACACTCACAGCCCTTCCCAGCCCCACCTCCAAGTAGTATGCAAATGGCACTGAGATTTAGCAAAAACGCGGC GTCTTTGAAAGTTAACAGACACTAAGGTAGCAGTGCCTGTGTGTTGGAGCTCAGTTAGGAGTTGAAGTTTTCTGCACCTGTGATAACAAGCCCACCAGCC ACATGCATTTCTTCTTCCTGCCACTGTGACTCGGTGGCTGTAGAGCCACATGGCATTTGTCAGCCAATCTGTTTCAAGTCTTTTCTTTCCACCAATAGCT TTCTTACAGATTTTCTTGGCTAAATGAGGCCATTACCTAGATTGTCCCTTCAGTAGGAGCCTGAATCCTTCGCTTACCAGGCTGAGAGCTATTTGCCATG ATAATGCTGATTCTCTCACTCCTCATGCAGAATGTGCACCTGCAGCCCTTTGATGCGTTTTGATTTGGGGGCGATTTTTCAGGCTTTGATTAGCAGGCTG CTTGCAATGCAGAAGATCACAGTTCCTTTCTGGGGCAGCAGCAACCAGAGCCTCTTCCTGTGTCCTGTGTGTCTGGTATGTTTACACCTTGCTCTGAGTT TTTCTCTTGTTTTCTTTTTTTAATTAAGTTTACATTTTAGTTTCTTTGACATCTTGTTTACAGTTTTTGTCTGATGTTTTTTCTTTTGTAGTGTCTTATG CCCTAGATTTAGTCATCTTGTTAAATATATTTGGATATGAAATCTTGACGAAAAGAAAATAACTCCTGAAAAAGAGGGTCTATCTTGCCACACGTTAAAA ACAAAAATAATGTCCTTGTGGCCTCTTACTTGATAAAGTTGCACCAGGACGGTCTGAGAAAGGCAGGCGGTGTAATATGTGGTGCAATCCATTTTTTCAA GGTGATGACGGCACAAAATACTTAGACTAGATGCTGATCCATAAACATTCACTGATATTCTTACAGGTTGGTTTGCAGTGTTAACATTTTTGTTAGCCTG ATCTGTAGGTGGGAAGTAAGATCATCATAATTCCTTAGCTCTGAGCACTGATTCAAAATTGTATAAATGACTGAACAACTTAGATGATTTGAAATTAATT GAAAATAAGAAGGGCTTGTGGAATTTGCATTTGGCTTTGGCCTTCAAGCATTTGGAAATATTAAGAGAATTTCCATTTTACATGTCTCCAACTGGGGTGC TTCTTACAACCCTTTCACTTTTTTTTTGAGACGAGTTTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCATCCTCTGCCTCC TGGGTTCAAGTGATTGTCCCGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGTGCACTACCATGCCCAGCTAATTTTTGTGTTTTTAGTAGAGACTGG GTTTCACCATGTTGGCCAGGCTCGTCTCAAACTCCTGACCTCAGGTGATCTACCCGCCTCCACCTCCCAAAGTGTTGTGATTACAGGCGTGAGCCACCAC ACCCAGCCCTTTCACTAATTTTTGGCAGAGTTCACAATCCCATCCTATGTCAGAAACTTGAACCTAACTTAAGAAATTTTGAGTCATTTTTTTGGAGGAC TGAGGCTTTGTTCTACCTACTCTTGAAGAATCCTGATGAAATATTCAAACACATAAAGGGATGGCCCTGGAGGTCTATCCTGCATACCTGGAGAGCTTCT CTGGTCCTGCCCACCTTGAAAGTTGCAGACTTTGTTGTAACATCTATGTGATGAGCTTCCCTGTGAAGCCCCATTTGACAGGTGGGCCAGCAGTGTTTTA TCAGCACTCTGATGGCCCACATTTCCCAGGAAAACTTCTTGAAACTTACCGTTGTGGGCAGTGGTCAGAGCGTGTAGTTCTTATGACTGTCTTGTTTCAT CTGTGGAGCACGTGTGGAGCACGTTGGTACAGCAGTTTCTGTGGGGTTTGGCATGGGTGATTTTGGTTAGTAACCATCCTCCAATTGTTGCTTGTGAAAG TCATTCATAACTTGTTCCTTTTACTTAGTTTTGTTGTTACTGCTGTTGTTGTTGGTTTGGTTGGTTTTGAGACCGGGTCTGGCTCTGTAGCCCAAGCTGG AATGCGGTGGCGCGATCATGGCTCCCTGCAGCCTCAAACTCCTGGGCTCAAGCAATCCTCCTGCCTCAGCCTCCCAAGCAGCTGGGAAGATAGGCACGTG CCACCACACCCGGCTAATTTTTTCTTTATTTTTTTTGTAGAGATGGGGGTCTCACTATGTTGCCCAGGCTGGTCTCAAACTCCTGGGCTCAAGCGATCCT TTGGCCTCGGCCTCCCAAAGTGCATGAGCCACCATGCCTGGCCTGTTTAGTTTTGTTTCAAGTTGAAATACCTTTCTTGTGTTTTCTAATTAGAAAAGTA ATATCTACTCATTGTAAAAACTCAAACAGTGCAGAAATGTAGAAAGTAGAAAGTGTAAGTCCCTGGTTGTCCCTTCTGCCTGAGACAACCACTGCTCACA GTTTGATGTATATCCTTCCAGAGACTCTCAAATTTAAGCAAATAATTTTTATTACCATGTCTTTTTATTTGAAGACGTACATTTGCCTCCAAAGTTCAAC ACAAGTTCAACTGACCATATCCTTCCATGACCTGAATAGATGCTATCCTTTATCACGATGTTCAATTGCCTTTGAAAGAGAGTAGTCCAGGTATATTCCT GATCAAAATTTGGCATTTTTGATGATACTACTCTACACAGATCAGACTCATGTGCAGAATCGTGCCTGGAGAGAGAGGTTTGGTTAAGACAGAGATTTCT GGAAACATTCAAATTGCAAATGGAAACTTGAAACCCACAATCTAATGAGGAATGTACTGGAAAAATAATCTGAAGAGTTGACAAATTGTGTACTAGATTG AACACATGGAATGCAATGCAATGAGACTTTCTGCACTAAAACTTATCCTCATATGTACAACAATGATGTGTGTATTATATAACAGTGATGTGTACATTTC TGACACCCCATACATAATATACACAGTTTGTATAAATGCATACATTTAAAAATATATATGTACAATACAGCTAACATAAAACTGTAGTACGCCTGAAGGA TATTACTAGTGCCTAATATTGAGTATGAGTCACTGCGTGTTCGCATCAACTTGGAAGTGCAGTAATTGTTATAAAATTAATCAGTGCAGCCAACATTATT TATGAATCACATCTTTGAAACTGTGCAGTAGCATATACATATATATTTTTAAATAACATTTTTCACAGTTTTCCAGAGTTACTGTTGAAATCTGCATCAC CAAAAAAAAAAAAAAGCAAGATTTTTTTAACAATGTAGACACTCTTCAGACCCAGTAATCTGCGTGTGATTTCCTATTTGTAGATTCCCAAGAGACTTTA GCAGTCACCAGCCTTAATGCATGTACAGGATATTATTGTGACTTAATTTATCTGCAGTTTTTAATCCATGTGAAATTGGAATTTATAAACGGACTTGGAT TAAACATGTCTGCCTTTCTAAGGTTGCAAATGTTACATTAAATGATTTATGTTGTAAATGAGAGAAGTTGAGTTGTACGTAAAAGACATCCACCAATTTC TGTGAATATTTTTACACGGAAACTTGAGAATCAAAATACGTTCAAGTCAGTTGTTCTGGCTCCCAGTTTATAATCTCGAAAAGCAATCTTTTTGTTTCAT AAATGGCTTAAATGTAGCTCCCACAGATTATCAGTATGAAGTCTGACATTTTTCATGGAAAAGAAGTATGTTTTTCTTCATTATAACTATAGTTTACTAT TTGTCTTTGCTATTTCCTGATACATACACATACGCACACACGCGCGCACATGCATGCACGTATGCACATGCACGCTCCCAGTGTCCCAGTGAAGGCTCAA CAGGAAGCTTGAGAAGGTCATCTCAATGGCCACAGGGTTCCTGTTCAACCAGTAGCCCTGGGATGCCGCTTTGCTTGTCATTCTTTAGTGTCACAAGTTA GTTCCCTGACTTGTGTGCAGTCAGGGAACTGCACACAACTGAAACCAACATGGTGGAATCATGTTTGCAATATTTGGTTGAGTAAAATAAGATAATTTTG AACTAAAGAAGACGGAGGCATTAGACCAGTGGCTTGACATGAAGCATCCCCTATGTGGGAGGGTAAGTTTGTTAACTTCATTTTTAGTCTTATCTCCAAC CCTTTTCCTTATCCTCCTCCCCAAACTAAAGAAGCAGAAACAGCAACAAAAGCAAAACCATCCCTTCACCTGATCCAGCCCATGTCCTTAGATTCAAATA TCCTTTGTCTTTTTCCCATTATACCATCTGACA

AFF3.F2 (forward primer)
AFF3.R31 (reverse primer)
Single exon skipping

Frame 0

KVPFRKLASEKRAPRSASEERRSTTYWGEKEKE RELRADHLPFQTGDGRQV*SRHPEASLSPFPRAG GPSLGGGGSGGGDADTSHHGQLRLSPAPGMGPR VTVCL*TR*KCITEERTRKKKSRNSTG*WHV*F *LLSLQ*ALQD*QGG*TLQPDPEHFRQL**NERL FN**IQSESSRWSSQTWGSSDSCEQDR*TFCCR FKSPEPALVYL*HYNFHTSSCPRAAE*ERHYGL AEGWAPTL*RPTESNTTGLSQDLAWRWCWQTAAS GQTSVQCGGGPSDPGEATCHGGQAQQQRTLCSE LSSIPSFKTQPGPAETDRVCEANGRPRSGP**V S*AEVVFGNQRALHIIQGSPCQQAGACQSQGQAL QVQHPQAGGGE*IWRNQQLC*RNNPGDDLASTT FCYSSTWQSGTNQISISK*GLSACILWTQ*SKE R*CRARESRQWHIEYINAGR*P*AKQ**RGE*TA GSSENGSPRSL*QRRGPAAQLQNLGAFQQGQQQ QQQQRQQQLLQRLREQLRI*LGDREQLQRE*GQ QAPPLLQPRG*TGIL*QVAAG*MAKQS*SPQASY SDPK*KPRVREQSVLQPGERGRPGLWESPRRLP AQPEREGDQEHLQGGAKAKDSQQGPWE*RREAE VPARGRGRGGERSRPATRSALCARGERARACPEV RGQEAHQAHREDLSRGRRQLPPARGARGRGRAG DERGGPPGAHQNQALWQQQSEPPQGAALLRDLR EAPHAGAKQDRPQIQGVH*DRVVIFILLLGLRPG VRAGGVPSVQSTDRGCLCLLRE*SEAEGGRCQR GQWS*GPCRLHQRQDHQ*HRQGAGGAVLHTGPL WPERTSLPSKGQ**DQVSLGQNRPDPPVQDPRTP APGARGIERPCHQGL*ERTAQPHLGHTCRKGFA KIQEETQV*QRRRLQGDQEVPGRERQLFKTGHL HQ*YFVCKPLQHEHQQCGNTNK*K*KNASVAHLT PL*CI*TQIHQRGLNFFQPT*WQQFVYFSLFQQ KA*GRQPAAASRRRPHESSSQQF*KHSPPQVTA ADEAVVSRLQRPQGLQEAETCLR*YASQCRLFYA RS*TNEA*SRCNGGKVWKGFELC*SSIVVYRVW KCNGTRPHGIQISLYDVFRNSRAHQVCYETKNP LRPQCHTRRQTTGCIMLPMPGPPVLADVSTQKGP RCKVFKSTNRLFQELI*SRPSPISVGGQWKEHW NPIPHVSQPLSRQLRGVSGQPLQRQRPVPVDHR QHPTAHPPDGGQPRQHHQQHPAQLRLLGDGRQPG QGKPRILQRPGSAHGAGHPAQQHGAPGPVLPTG PALAAEQRPPVIGTSPWGQSGLWSPQMAQRFWT LCY*NSQPQHLSDCGEHFLSIEQ*SFAGHVCLYV WVYKKPPVYVCKIHSSSEHTFFV*FP*SQARQS DQRFLTRGSTHTHTHTHTHRTLTHNHRHTLSIP FQC*TVTS*KIQPKSHGLVNQVQYSTPKA*LPVK MDLLLYC*LLSVLIYYSPIIF*LVCING*LNNE NKPGIFVPEVY*IACYQIPCSSSNSLPPSDSII N*MHSRQTDGHSQPFPAPPPSSMQMALRFSKNAA SLKVNRH*GSSACVLELS*ELKFSAPVITSPPA TCISSSCHCDSVAVEPHGICQPICFKSFLSTNS FLTDFLG*MRPLPRLSLQ*EPESFAYQAESYLP* *C*FSHSSCRMCTCSPLMRFDLGAIFQALISRL LAMQKITVPFWGSSNQSLFLCPVCLVCLHLALS FSLVFFF*LSLHFSFFDILFTVFV*CFFFCSVLC PRFSHLVKYIWI*NLDEKKITPEKEGLSCHTLK TKIMSLWPLT**SCTRTV*ERQAV*YVVQSIFS R**RHKILRLDADP*TFTDILTGWFAVLTFLLA* SVGGK*DHHNSLALSTDSKLYK*LNNLDDLKLI ENKKGLWNLHLALAFKHLEILREFPFYMSPTGV LLTTLSLFF*DEFRSVAQAGVQWHDLGSLHPLPP GFK*LSRLSLPSSWDYRCALPCPANFCVFSRDW VSPCWPGSSQTPDLR*STRLHLPKCCDYRREPP HPALSLIFGRVHNPILCQKLEPNLRNFESFFWRT EALFYLLLKNPDEIFKHIKGWPWRSILHTWRAS LVLPTLKVADFVVTSM**ASL*SPI*QVGQQCF ISTLMAHISQENFLKLTVVGSGQSV*FL*LSCFI CGARVEHVGTAVSVGFGMGDFG**PSSNCCL*K SFITCSFYLVLLLLLLLLVWLVLRPGLAL*PKL ECGGAIMAPCSLKLLGSSNPPASASQAAGKIGTC HHTRLIFSLFFL*RWGSHYVAQAGLKLLGSSDP LASASQSA*ATMPGLFSFVSS*NTFLVFSN*KS NIYSL*KLKQCRNVESRKCKSLVVPSA*DNHCSQ FDVYPSRDSQI*ANNFYYHVFLFEDVHLPPKFN TSSTDHILP*PE*MLSFITMFNCL*KRVVQVYS *SKFGIFDDTTLHRSDSCAESCLEREVWLRQRFL ETFKLQMET*NPQSNEECTGKII*RVDKLCTRL NTWNAMQ*DFLH*NLSSYVQQ*CVYYITVMCTF LTPHT*YTQFV*MHTFKNIYVQYS*HKTVVRLKD ITSA*Y*V*VTACSHQLGSAVIVIKLISAANII YESHL*NCAVAYTYIFLNNIFHSFPELLLKSAS PKKKKSKIFLTM*TLFRPSNLRVISYL*IPKRL* QSPALMHVQDIIVT*FICSF*SM*NWNL*TDLD *TCLPF*GCKCYIK*FML*MREVELYVKDIHQF L*IFLHGNLRIKIRSSQLFWLPVYNLEKQSFCFI NGLNVAPTDYQYEV*HFSWKRSMFFFIITIVYY LSLLFPDTYTYAHTRAHACTYAHARSQCPSEGS TGSLRRSSQWPQGSCSTSSPGMPLCLSFFSVTS* FPDLCAVRELHTTETNMVESCLQYLVE*NKIIL N*RRRRH*TSGLT*SIPYVGG*VC*LHF*SYLQ PFSLSSSPN*RSRNSNKSKTIPSPDPAHVLRFKY PLSFSHYTI*

Frame 1

KCHSESSPARRELHAQPQKKDVQRLTGERKKRN GS*ELITCLSRPETGGKFEVDTQRPVCPPSRAR AARASAAAVAAAATLTPPTMDSFDLALLQEWDLE SLCVYEPDRNALRRKERERRNQETQQDDGTFNS SYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKD FLTDRSNQSHLVGVPKPGVPQTPVNKIDEHFVAD SRAQNQPSSICSTTTSTPAAVPVQQSKRGTMGW QKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQP RAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQN FPPSLASKPSLVQQKPTAYVRPMDGQDQAPDES PKLKSSSETSVHCTSYRGVPASKPEPARAKAKL SKFSIPKQGEESRSGETNSCVEEIIREMTWLPPL SAIQAPGKVEPTKFPFPNKDSQLVSSGHNNPKK GDAEPESPDSGTSNTSMLEDDLKLSSDEEENEQ QAAQRTALRALSDSAVVQQPNCRTSVPSSKGSSS SSSSGSSSSSSDSESSSGSDSETESSSSESEGS KPPHFSSPEAEPASSNKWQLDKWLNKVNPHKPP ILIQNESHGSESNQYYNPVKEDVQDCGKVPDVCQ PSLREKEIKSTCKEEQRPRTANKAPGSKGVKQK SPPAAVAVAVSAAAPPPAVPCAPAENAPAPARR SAGKKPTRRTERTSAGDGANCHRPEEPAAADALG TSVVVPPEPTKTRPCGNNRASHRKELRSSVTCE KRRTRGLSRIVPKSKEFIETESSSSSSSSDSDL ESEQEEYPLSKAQTVAASASSGNDQRLKEAAANG GSGPRAPVGSINARTTSDIAKELEEQFYTLVPF GRNELLSPLKDSDEIRSLWVKIDLTLLSRIPEH LPQEPGVLSAPATKDSESAPPSHTSDTPAEKALP KSKRKRKCDNEDDYREIKKSQGEKDSSSRLATS TSNTLSANHCNMNINSVAIPINKNEKMLRSPIS PLSDASKHKYTSEDLTSSSRPNGNSLFTSASSSK KPKADSQLQPHGGDLTKAAHNNSENIPLHKSRP QTKPWSPGSNGHRDCKRQKLVFDDMPRSADYFM QEAKRMKHKADAMVEKFGKALNYAEAALSFIECG NAMEQGPMESKSPYTMYSETVELIRYAMRLKTH SGPNATPEDKQLAALCYRCLALLYWRMFRLKRD HAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTG TPSPMSPNPSPASSVGSQGSLSNASALSPSTIV SIPQRIHQMAANHVSITNSILHSYDYWEMADNL AKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQG LHWLRNSAHLS*GPHPGARVGSGLHRWLNVFGH CATETPSHSIYQTAVNISSALNNDLLQGMCVCM CGYIRSRLCMCVRYTALQNTPSLSSFHNPRLDKV IRGF*LEEVHTHTHTHTHTVHSHTITDTHFLFH SSAKP*LLRKFNQNLMG*LTRCNTAHQKLDCQ* RWTCSYTVDYFQY*YTIPQLFFDWYVLMGN*IMK ISQVFLCRKFTRLHVTKYPALPLIPFPHLTLLS TKCIPGKQMDTHSPSQPHLQVVCKWH*DLAKTR RL*KLTDTKVAVPVCWSSVRS*SFLHL**QAHQP HAFLLPATVTRWL*SHMAFVSQSVSSLFFPPIA FLQIFLAK*GHYLDCPFSRSLNPSLTRLRAICH DNADSLTPHAECAPAAL*CVLIWGRFFRL*LAGC LQCRRSQFLSGAAATRASSCVLCVWYVYTLL*V FLLFSFFN*VYILVSLTSCLQFLSDVFSFVVSY ALDLVILLNIFGYEILTKRK*LLKKRVYLATR*K QK*CPCGLLLDKVAPGRSEKGRRCNMWCNPFFQ GDDGTKYLD*MLIHKHSLIFLQVGLQC*HFC*P DL*VGSKIIIIP*L*ALIQNCIND*TT*MI*N*L KIRRACGICIWLWPSSIWKY*ENFHFTCLQLGC FLQPFHFFFETSFALLPRLECSGTISAHCILCL LGSSDCPASASQVAGITGVHYHAQLIFVFLVETG FHHVGQARLKLLTSGDLPASTSQSVVITGVSHH TQPFH*FLAEFTIPSYVRNLNLT*EILSHFFGG LRLCSTYS*RILMKYSNT*RDGPGGLSCIPGELL WSCPP*KLQTLL*HLCDELPCEAPFDRWASSVL SAL*WPTFPRKTS*NLPLWAVVRACSSYDCLVS SVEHVWSTLVQQFLWGLAWVILVSNHPPIVACES HS*LVPFT*FCCYCCCCWFGWF*DRVWLCSPSW NAVARSWLPAASNSWAQAILLPQPPKQLGR*AR ATTPG*FFLYFFCRDGGLTMLPRLVSNSWAQAIL WPRPPKVHEPPCLACLVLFQVEIPFLCFLIRKV ISTHCKNSNSAEM*KVESVSPWLSLLPETTTAH SLMYILPETLKFKQIIFITMSFYLKTYICLQSST QVQLTISFHDLNRCYPLSRCSIAFERE*SRYIP DQNLAFLMILLYTDQTHVQNRAWRERFG*DRDF WKHSNCKWKLETHNLMRNVLEK*SEELTNCVLD* THGMQCNETFCTKTYPHMYNNDVCII*Q*CVHF *HPIHNIHSLYKCIHLKIYMYNTANIKL*YA*R ILLVPNIEYESLRVRINLEVQ*LL*N*SVQPTLF MNHIFETVQ*HIHIYF*ITFFTVFQSYC*NLHH QKKKKARFF*QCRHSSDPVICV*FPICRFPRDF SSHQP*CMYRILL*LNLSAVFNPCEIGIYKRTWI KHVCLSKVANVTLNDLCCK*EKLSCT*KTSTNF CEYFYTET*ESKYVQVSCSGSQFIISKSNLFVS *MA*M*LPQIISMKSDIFHGKEVCFSSL*L*FTI CLCYFLIHTHTHTRAHMHARMHMHAPSVPVKAQ QEA*EGHLNGHRVPVQPVALGCRFACHSLVSQV SSLTCVQSGNCTQLKPTWWNHVCNIWLSKIR*F* TKEDGGIRPVA*HEASPMWEGKFVNFIFSLISN PFPYPPPQTKEAETATKAKPSLHLIQPMSLDSN ILCLFPIIPSD

Frame 2

SAIQKARQREESSTLSLRRKTFNDLLGRERKGT GAES*SPAFPDRRRAASLKSTPRGQFVPLPARG RPEPRRRR*RRRRR*HLPPWTAST*PCSRNGTS SHCVSMNQIEMHYGGKNEKEEIKKLNRMMARLIL VTLSSVSPTRLTRGMNSPTGSRTL*AIMMK*KT F*LIDPIRVISLEFPNLGFLRLL*TRSMNILLQ IQEPRTSPRLSVALQLPHQQLSPCSRVREALWAG RRLGTHPLTANREQHNRALSGPCLEMVLADSSL GPNKCAMWRWAFRPRRGHLPWRPSTAAADTVFR TFLHP*LQNPAWSSRNRPRM*GQWTAKIRPLMSL LS*SRLRKPACTAHHTGESLPASRSLPEPRPSS PSSASPSRGRRVDLEKPTAVLKK*SGR*PGFHH FLLFKHLAKWNQPNFHFQIRTLSLYPLDTIIQRK VMQSQRVQTVAHRIHQCWKMTLS*AVMKRRMNS RQLRERLSALSLTAPWSSSPTAEPRCLPARAAA AAAAAAAAAPPATQRAAPDLTRRPRAAPARVRAA SPPTSPAPRLNRHPLTSGSWING*TKLIPTSLL F*SKMKATGQRAISTTTR*KRTSRTVGKSPTFA SPA*ERRRSRALARRSKGQGQPTRPLGVKA*SRS PRPRPWPWR*AQPPRHPQCPVRPRRTRPRLPGG PRARSPPGAPRGPQPGTAPTATGPRSPRPRTRW GRAWWSPRSPPKPGPVATTERATARSCAPP*PAR SAARGG*AGSSPNPRSSLRQSRHLHPPPRTPTW SPSRRSTLCPKHRPWLPLPPPGMIRG*RRPLPT GAVVLGPL*APSTPGPPVTSPRSWRSSSTHWSPL AGTNFSPL*RTVMRSGLSGSKST*PSCPGSQNT CPRSQGY*APLPPRTLRAHRPATPRTHLQKRLC QNPRGNASVTTKTTTGRSRSPRERKTALQDWPPP PVILCLQTTAT*TSTVWQYQ*IKMKKCFGRPSH PSLMHLNTNTPART*LLPADLMATVCLLQPLPA KSLRPTASCSLTAETSRKQLTTILKTFPSTSHGR RRSRGLQAPTATGTARGRNLSSMICLAVPIILC KKLNE*SIKQMQWWKSLERL*TMLKQHCRLSSV EMQWNKAPWNPNLLIRCIQKQ*SSSGML*D*KPT QAPMPHQKTNNWLHYVTDAWPSCTGGCFDSKGT TL*SIQKH*STISRTHLKPPKPHLRGGPVERAL EPHPPCLPTPLPPAPWGLRAASPTPAPCPRRPSS ASHSASTRWRPTTSASPTASCTATTTGRWPTTW PRKTENSSTTWICSWGRSPCTAAWSTWSSTPNR ACTGCGTAPTCHRDLTLGPEWALVSTDGSTFLDT VLLKLPATAFIRLR*TFPQH*TMIFCRACVFVC VGI*EAACVCV*DTQLFRTHLLCLVSIIPG*TK *SEVSD*RKYTHTHTHTHTPYTHTQSQTHTFYSI PVLNRDFLENSTKISWVS*PGAIQHTKSLTASE DGPALILLITFSINILFPNYFLIGMY*WVIE** K*ARYFCAGSLLDCMLPNTLLFL*FPSPI*LYYQ LNAFQANRWTLTALPSPTSK*YANGTEI*QKRG VFES*QTLR*QCLCVGAQLGVEVFCTCDNKPTS HMHFFFLPL*LGGCRATWHLSANLFQVFSFHQ*L SYRFSWLNEAIT*IVPSVGA*ILRLPG*ELFAM IMLILSLLMQNVHLQPFDAF*FGGDFSGFD*QA ACNAEDHSSFLGQQQPEPLPVSCVSGMFTPCSEF FSCFLFLIKFTF*FL*HLVYSFCLMFFLL*CLM P*I*SSC*IYLDMKS*RKENNS*KRGSILPHVK NKNNVLVASYLIKLHQDGLRKAGGVICGAIHFFK VMTAQNT*TRC*SINIH*YSYRLVCSVNIFVSL ICRWEVRSS*FLSSEH*FKIV*MTEQLR*FEIN *K*EGLVEFAFGFGLQAFGNIKRISILHVSNWGA SYNPFTFFLRRVSLCCPGWSAVARSRLTASSAS WVQVIVPPQPPK*LGLQVCTTMPS*FLCF**RL GFTMLARLVSNS*PQVIYPPPPPKVL*LQA*ATT PSPFTNFWQSSQSHPMSET*T*LKKF*VIFLED *GFVLPTLEES**NIQTHKGMALEVYPAYLESF SGPAHLESCRLCCNIYVMSFPVKPHLTGGPAVFY QHSDGPHFPGKLLETYRCGQWSERVVLMTVLFH LWSTCGARWYSSFCGVWHG*FWLVTILQLLLVK VIHNLFLLLSFVVTAVVVGLVGFETGSGSVAQAG MRWRDHGSLQPQTPGLKQSSCLSLPSSWEDRHV PPHPANFFFIFFVEMGVSLCCPGWSQTPGLKRS FGLGLPKCMSHHAWPV*FCFKLKYLSCVF*LEK* YLLIVKTQTVQKCRK*KV*VPGCPFCLRQPLLT V*CISFQRLSNLSK*FLLPCLFI*RRTFASKVQ HKFN*PYPSMT*IDAILYHDVQLPLKESSPGIFL IKIWHF**YYSTQIRLMCRIVPGERGLVKTEIS GNIQIANGNLKPTI**GMYWKNNLKS*QIVY*I EHMECNAMRLSALKLILICTTMMCVLYNSDVYIS DTPYIIYTVCINAYI*KYICTIQLT*NCSTPEG YY*CLILSMSHCVFASTWKCSNCYKINQCSQHY L*ITSLKLCSSIYIYIFK*HFSQFSRVTVEICIT KKKKKQDFFNNVDTLQTQ*SACDFLFVDSQETL AVTSLNACTGYYCDLIYLQFLIHVKLEFINGLG LNMSAFLRLQMLH*MIYVVNERS*VVRKRHPPIS VNIFTRKLENQNTFKSVVLAPSL*SRKAIFLFH KWLKCSSHRLSV*SLTFFMEKKYVFLHYNYSLL FVFAIS*YIHIRTHARTCMHVCTCTLPVSQ*RLN RKLEKVISMATGFLFNQ*PWDAALLVIL*CHKL VP*LVCSQGTAHN*NQHGGIMFAIFG*VK*DNF ELKKTEALDQWLDMKHPLCGRVSLLTSFLVLSPT LFLILLPKLKKQKQQQKQNHPFT*SSPCP*IQI SFVFFPLYHLT

Sequence AFF3.bAug05

AAATCCGATCTCAAATAGGAAAGAAGTCTCATTGGCCTTTTGGTCTTCTACCTGTGTCCTGAATTGAGTTTGTTAATCCTCATTCGGAGCTGATCACCTG CCTTTCCAGACCGGAGACGGGCGGCAAGTTTGAAGTCGACACCCAGAGGCCAGTTTGTCCCCCTTCCCGCGCGCGGGCGGCCCGAGCCTCGGCGGCGGCG GTAGCGGCGGCGGCGACGCTGACACCTCCCACCATGGACAGCTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGAGTCACTGTGGGGTGAAGACATCT TAAACCAGAGGAATGACTCTCTAGTTGTGGAATTTCAGTCATCAGCCAGCAGATGCAGGAGTGTCTATGAACCAGATAGAAATGCATTACGGAGGAAAGA ACGAGAAAGAAGAAATCAAGAAACTCAACAGGATGATGGCACGTTTAATTCTAGTTACTCTCTCTTCAGTGAGCCCTACAAGACTAACAAGGGGGATGAA CTCTCCAACCGGATCCAGAACACTTTAGGCAATTATGATGAAATGAAAGACTTTTTAACTGATAGATCCAATCAGAGTCATCTCGTTGGAGTTCCCAAAC CTGGGGTTCCTCAGACTCCTGTGAACAAGATCGATGAACATTTTGTTGCAGATTCAAGAGCCCAGAACCAGCCCTCGTCTATCTGTAGCACTACAACTTC CACACCAGCAGCTGTCCCCGTGCAGCAGAGTAAGAGAGGCACTATGGGCTGGCAGAAGGCTGGGCACCCACCCTCTGACGGCCAACAGAGAGCAACACAA CAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTGTTGGCAGACAGCAGCCTCGGGCCAAACAAGTGTGCAATGTGGAGGTGGGCCTTCAGACCCAGGAGA GGCCACCTGCCATGGCGGCCAAGCACAGCAGCAGCGGACACTGTGTTCAGAACTTTCCTCCATCCCTAGCTTCAAAACCCAGCCTGGTCCAGCAGAAACC GACCGCGTATGTGAGGCCAATGGACGGCCAAGATCAGGCCCCTGATGAGTCTCCTAAGCTGAAGTCGTCTTCGGAAACCAGCGTGCACTGCACATCATAC AGGGGAGTCCCTGCCAGCAAGCCGGAGCCTGCCAGAGCCAAGGCCAAGCTCTCCAAGTTCAGCATCCCCAAGCAGGGGGAGGAGAGTAGATCTGGAGAAA CCAACAGCTGTGTTGAAGAAATAATCCGGGAGATGACCTGGCTTCCACCACTTTCTGCTATTCAAGCACCTGGCAAAGTGGAACCAACCAAATTTCCATT TCCAAATAAGGACTCTCAGCTTGTATCCTCTGGACACAATAATCCAAAGAAAGGTGATGCAGAGCCAGAGAGTCCAGACAGTGGCACATCGAATACATCA ATGCTGGAAGATGACCTTAAGCTAAGCAGTGATGAAGAGGAGAATGAACAGCAGGCAGCTCAGAGAACGGCTCTCCGCGCTCTCTCTGACAGCGCCGTGG TCCAGCAGCCCAACTGCAGAACCTCGGTGCCTTCCAGCAAGGGCAGCAGCAGCAGCAGCAGCAGCGGCAGCAGCAGCTCCTCCAGCGACTCAGAGAGCAG CTCCGGATCTGACTCGGAGACCGAGAGCAGCTCCAGCGAGAGTGAGGGCAGCAAGCCCCCCCACTTCTCCAGCCCCGAGGCTGAACCGGCATCCTCTAAC AAGTGGCAGCTGGATAAATGGCTAAACAAAGTTAATCCCCACAAGCCTCCTATTCTGATCCAAAATGAAAGCCACGGGTCAGAGAGCAATCAGTACTACA ACCCGGTGAAAGAGGACGTCCAGGACTGTGGGAAAGTCCCCGACGTTTGCCAGCCCAGCCTGAGAGAGAAGGAGATCAAGAGCACTTGCAAGGAGGAGCA AAGGCCAAGGACAGCCAACAAGGCCCCTGGGAGTAAAGGCGTGAAGCAGAAGTCCCCGCCCGCGGCCGTGGCCGTGGCGGTGAGCGCAGCCGCCCCGCCA CCCGCAGTGCCCTGTGCGCCCGCGGAGAACGCGCCCGCGCCTGCCCGGAGGTCCGCGGGCAAGAAGCCCACCAGGCGCACCGAGAGGACCTCAGCCGGGG ACGGCGCCAACTGCCACCGGCCCGAGGAGCCCGCGGCCGCGGACGCGCTGGGGACGAGCGTGGTGGTCCCCCCGGAGCCCACCAAAACCAGGCCCTGTGG CAACAACAGAGCGAGCCACCGCAAGGAGCTGCGCTCCTCCGTGACCTGCGAGAAGCGCCGCACGCGGGGGCTAAGCAGGATCGTCCCCAAATCCAAGGAG TTCATTGAGACAGAGTCGTCATCTTCATCCTCCTCCTCGGACTCCGACCTGGAGTCCGAGCAGGAGGAGTACCCTCTGTCCAAAGCACAGACCGTGGCTG CCTCTGCCTCCTCCGGGAATGATCAGAGGCTGAAGGAGGCCGCTGCCAACGGGGGCAGTGGTCCTAGGGCCCCTGTAGGCTCCATCAACGCCAGGACCAC CAGTGACATCGCCAAGGAGCTGGAGGAGCAGTTCTACACACTGGTCCCCTTTGGCCGGAACGAACTTCTCTCCCCTCTAAAGGACAGTGATGAGATCAGG TCTCTCTGGGTCAAAATCGACCTGACCCTCCTGTCCAGGATCCCAGAACACCTGCCCCAGGAGCCAGGGGTATTGAGCGCCCCTGCCACCAAGGACTCTG AGAGCGCACCGCCCAGCCACACCTCGGACACACCTGCAGAAAAGGCTTTGCCAAAATCCAAGAGGAAACGCAAGTGTGACAACGAAGACGACTACAGGGA GATCAAGAAGTCCCAGGGAGAGAAAGACAGCTCTTCAAGACTGGCCACCTCCACCAGTAATACTTTGTCTGCAAACCACTGCAACATGAACATCAACAGT GTGGCAATACCAAT

AFF3.F2 (forward primer)
AFF3.R31 (reverse primer)
Single exon skipping

Frame 0

KSDLK*ERSLIGLLVFYLCPELSLLILIRS*SP AFPDRRRAASLKSTPRGQFVPLPARGRPEPRRRR *RRRRR*HLPPWTAST*PCSRNGTSSHCGVKTS *TRGMTL*LWNFSHQPADAGVSMNQIEMHYGGK NEKEEIKKLNRMMARLILVTLSSVSPTRLTRGMN SPTGSRTL*AIMMK*KTF*LIDPIRVISLEFPN LGFLRLL*TRSMNILLQIQEPRTSPRLSVALQL PHQQLSPCSRVREALWAGRRLGTHPLTANREQHN RALSGPCLEMVLADSSLGPNKCAMWRWAFRPRR GHLPWRPSTAAADTVFRTFLHP*LQNPAWSSRN RPRM*GQWTAKIRPLMSLLS*SRLRKPACTAHHT GESLPASRSLPEPRPSSPSSASPSRGRRVDLEK PTAVLKK*SGR*PGFHHFLLFKHLAKWNQPNFH FQIRTLSLYPLDTIIQRKVMQSQRVQTVAHRIHQ CWKMTLS*AVMKRRMNSRQLRERLSALSLTAPW SSSPTAEPRCLPARAAAAAAAAAAAAPPATQRA APDLTRRPRAAPARVRAASPPTSPAPRLNRHPLT SGSWING*TKLIPTSLLF*SKMKATGQRAISTT TR*KRTSRTVGKSPTFASPA*ERRRSRALARRS KGQGQPTRPLGVKA*SRSPRPRPWPWR*AQPPRH PQCPVRPRRTRPRLPGGPRARSPPGAPRGPQPG TAPTATGPRSPRPRTRWGRAWWSPRSPPKPGPV ATTERATARSCAPP*PARSAARGG*AGSSPNPRS SLRQSRHLHPPPRTPTWSPSRRSTLCPKHRPWL PLPPPGMIRG*RRPLPTGAVVLGPL*APSTPGP PVTSPRSWRSSSTHWSPLAGTNFSPL*RTVMRSG LSGSKST*PSCPGSQNTCPRSQGY*APLPPRTL RAHRPATPRTHLQKRLCQNPRGNASVTTKTTTG RSRSPRERKTALQDWPPPPVILCLQTTAT*TSTV WQYQ

Frame 1

NPISNRKEVSLAFWSSTCVLN*VC*SSFGADHL PFQTGDGRQV*SRHPEASLSPFPRAGGPSLGGG GSGGGDADTSHHGQLRLSPAPGMGPRVTVG*RHL KPEE*LSSCGISVISQQMQECL*TR*KCITEER TRKKKSRNSTG*WHV*F*LLSLQ*ALQD*QGG* TLQPDPEHFRQL**NERLFN**IQSESSRWSSQT WGSSDSCEQDR*TFCCRFKSPEPALVYL*HYNF HTSSCPRAAE*ERHYGLAEGWAPTL*RPTESNT TGLSQDLAWRWCWQTAASGQTSVQCGGGPSDPGE ATCHGGQAQQQRTLCSELSSIPSFKTQPGPAET DRVCEANGRPRSGP**VS*AEVVFGNQRALHII QGSPCQQAGACQSQGQALQVQHPQAGGGE*IWRN QQLC*RNNPGDDLASTTFCYSSTWQSGTNQISI SK*GLSACILWTQ*SKER*CRARESRQWHIEYI NAGR*P*AKQ**RGE*TAGSSENGSPRSL*QRRG PAAQLQNLGAFQQGQQQQQQQRQQQLLQRLREQ LRI*LGDREQLQRE*GQQAPPLLQPRG*TGIL* QVAAG*MAKQS*SPQASYSDPK*KPRVREQSVLQ PGERGRPGLWESPRRLPAQPEREGDQEHLQGGA KAKDSQQGPWE*RREAEVPARGRGRGGERSRPA TRSALCARGERARACPEVRGQEAHQAHREDLSRG RRQLPPARGARGRGRAGDERGGPPGAHQNQALW QQQSEPPQGAALLRDLREAPHAGAKQDRPQIQG VH*DRVVIFILLLGLRPGVRAGGVPSVQSTDRGC LCLLRE*SEAEGGRCQRGQWS*GPCRLHQRQDH Q*HRQGAGGAVLHTGPLWPERTSLPSKGQ**DQ VSLGQNRPDPPVQDPRTPAPGARGIERPCHQGL* ERTAQPHLGHTCRKGFAKIQEETQV*QRRRLQG DQEVPGRERQLFKTGHLHQ*YFVCKPLQHEHQQ CGNTN

Frame 2

IRSQIGKKSHWPFGLLPVS*IEFVNPHSELITC LSRPETGGKFEVDTQRPVCPPSRARAARASAAA VAAAATLTPPTMDSFDLALLQEWDLESLWGEDI LNQRNDSLVVEFQSSASRCRSVYEPDRNALRRKE RERRNQETQQDDGTFNSSYSLFSEPYKTNKGDE LSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPK PGVPQTPVNKIDEHFVADSRAQNQPSSICSTTTS TPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQ QGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQE RPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKP TAYVRPMDGQDQAPDESPKLKSSSETSVHCTSY RGVPASKPEPARAKAKLSKFSIPKQGEESRSGE TNSCVEEIIREMTWLPPLSAIQAPGKVEPTKFPF PNKDSQLVSSGHNNPKKGDAEPESPDSGTSNTS MLEDDLKLSSDEEENEQQAAQRTALRALSDSAV VQQPNCRTSVPSSKGSSSSSSSGSSSSSSDSESS SGSDSETESSSSESEGSKPPHFSSPEAEPASSN KWQLDKWLNKVNPHKPPILIQNESHGSESNQYY NPVKEDVQDCGKVPDVCQPSLREKEIKSTCKEEQ RPRTANKAPGSKGVKQKSPPAAVAVAVSAAAPP PAVPCAPAENAPAPARRSAGKKPTRRTERTSAG DGANCHRPEEPAAADALGTSVVVPPEPTKTRPCG NNRASHRKELRSSVTCEKRRTRGLSRIVPKSKE FIETESSSSSSSSDSDLESEQEEYPLSKAQTVA ASASSGNDQRLKEAAANGGSGPRAPVGSINARTT SDIAKELEEQFYTLVPFGRNELLSPLKDSDEIR SLWVKIDLTLLSRIPEHLPQEPGVLSAPATKDS ESAPPSHTSDTPAEKALPKSKRKRKCDNEDDYRE IKKSQGEKDSSSRLATSTSNTLSANHCNMNINS VAIP