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Sequences

Sequence PAXIP1.aAug05

GTCCGTGGTAGGAATGCATCTTAGTTGAATATCCTCTTCAGGTTAATCTTCAAAATATTTCCTTCTTAAAAGTTTAGCTGTACAGTTGTGTTGCCTTGGG TGTGACATCAGTGGCCCCCACCTTGGAGGATGGGGATCCCCAGTGATCAGAGTTAGGCCTGGAAGGGCGGAGACTCATATTTCGCAGATGAACTTGGATG GTGTAGAAGGAATTGTAGAAGGCTGAGTGATCTTGGCCTTCTTGAGGTTCGTCCTTGTAACAACTTTTTGGACCCTTTTTGCCCTGTAACTTTAGGGTAA AGAGACTTCAAGGCCGGGTTTACCCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACCTGAGGTCATGAGTTCGAGACCAGCCTGGCCAA CATGGTGAAGCCTCATCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGGGCGCCTGTGGTCTCAGCTACTCGGGAGGCTGAGGCAGGAGAATT GCTGGAACCTGGGAGGTGGAAGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCAACAACAGCTAGACTCCGTCTCAAAAAAAAAAAAAA AGCCTTCTATCTTTGAATTTACAGAGTAAAACAGTTGCTGAACACGGAGCGATGAAGATGAGTGCCTGTAACAGTGTTAGCTTTTTCCAGTATTGATGAT TCCCCCGCCCCTCTTTTTTTTGTTTGTTTTTTTGTTTTTGTTTTTTGCTATCCACCATTTTTCTCTTCCCCCTCCTTCCTAGCCTTCGTGTTTCATTTTA GGACTTTGGCTCTTATAGGGGACTTGAACCAAATGTTACTTAAAACCTGGAACAAGTTGAACTGGAACCATATTTACTCGACTTTGAATCAAATCTAAGT GCTTAAAAATGTCCTTGGCAAACCACTTTCATCTGAAACAGTATATTTTCTGAAAAGTTTTGAGCCTGGACATCTGATTCTCTGACTCTTATGACTGTGG ATTATAAGTTCACATGTTGACCATCTAATATGAGGCAGGCATGGACTAGGTGCCAGGTATAATACAGAGGTGAAGAGGACATGGTACCTGCCCTTTAGCG CCCCTCATTGGTGGGGGAGGGAGCACACCTGGACATATCACAGTACAAGGTGTAGCAGATTTCTCCACTGGGGTGCAAAGTAGACACCACTGAAATGTCA CTCAGTAGATGTCATTTCAGTTGGGTTTTGAGATAAGCAGAAGTTAAGAACATGAAGGAATATTTTCATGTGGAGCGTATGTGCTGAGGAATGAAGGCAT TCATGCGTTTGGGATACATCAGGATGATCACTATATCTGGAACAGAGTGATATGAGATAAATCTATTAAGATACTCAGTAGTGTGGAGATTGAAGAGAAG CTATTGGTTTTGGCATCCTCAAAGTTCGTGAAAACCGAGTAAAGTGTTTTTGGTAGAGTGTTGGTGGAAGGGGCTGGATTGCAGGGAGTTGAAGAGTGAA TGGAAACTGAGAACAGAAAGGTTAGACTCTTCAGGAAGTTTGTCCTGAGAAGGAAATAGAGAAGGTGCCTGAGGAAGAGAGGGCTGGGGAGGGATCTGTG TTGCATCACAAGCGCGTGGAAGGATGTGGGTGACTTTACTGTTTACAGGTGGAGGGAACATAGCTATGGACAGGGAAACTCATGAAGAAGGGAGTAGGAT CAGAAACAGACTGGAGGATAGGAAAAGGAGGGGTGCCTTTTCTGAAGACCAGTCGAATGGATGCGTGAAGCTAAAAATGCTTAGAGGTCAAGGGAAGGAA GAAAGGTAAAGTGGCCCATGGCCAGTGACCGCTGTTTTTTCACTTACTAAATATTGAGCCTCTGCTCTAGTTCATATCTATTAGTGGTCGGTAAAGTATT CTGAAAAGGTTGTCTAGCTTGTGGGTGACTTTATTTTTTTTTGAGGTTGGAGCAAAAGTTTAATAAGTGAAAGAAGAAAGCTCTCTGCTGCAGAGAGGGG ACCTGGAAGAGCGTTGCCCTGGTTATTCAGCTTCTCAAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACATAATCTCAGAGGATG GGGACAATCCAGAGGTGGGAGAAGCTCGGGAAGTCTTTGACTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGTGGAACTCTTCTGCC AGTAAATGGTTTTTCTCCAGAATCATGTCAGATTTTTTTTGGAATCACTGCCTGCCTTTCTCAGGTGTCATCTGAAGACAGAAGTGCCCTGTGGGCTTTG GTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTCAATAAGAAATGCACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATACGAATGTGCTTTAA AGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGGTTCTGGATTGCGTATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCATCCTCGTCTGATTAT TTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGAGGAAGTAGAAAATGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATGAGAAGTCAAGCCCT GCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAGCAGTTTTCACCTAAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTTTGATGATTCTTCAG ATTCATCACCGGAAAAACAGGAGAGAAATTTAAACTGGACCCCGGCCGAAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCTCAGGGAAAGGAGCC TGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGTCCCAGGTAACATTTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTGGACAAAACCTCCAA AGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGTCCAGCTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCAGATGAACCGGCCAT CAAATGTAGCACATATCTTACAGACTCTTTCAGCACCTACGAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACAAATGCCAATGCAGT GCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACACATGCTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGGTTTTACACCTTCAG CCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAGCAGCAGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTCACAGCAACAGCAGC AGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGCAACAGCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCATCGCCCTCAGCAGCA GCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCAGCAGTTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTTGCCCAGCTCCAGCAGCAG CACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATTCAGCAGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGATGCAAAGTCAGACAG CGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGCATCAGGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCACCACCATCGCCTCA GCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGAGATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCCAGAGCAGATG TCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCACCTTCTCTGTG AGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGAAGAAAATGGT ACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTGTTGATAGTGAC AGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAAGAACCAACTGGTT TAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTGTGTCAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCACTGAGGCAGATTCA GTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCATTTGCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGTTCCCTTAAAAGTG TCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCAAACTGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGAATTGAAGACG TACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATTAAGAAGCTCTA CATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCTCATTGCCAGCAAAGTGACTCGCACCGTGAAGTTCCTGACGGCGATTTCTGTC GTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGATGCTGAGGCAGAAG TACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAG TCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGGAGGAAAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGT TTGTCGGAAATAATTTTAATATCCTGTGAAAATGACCTTCATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGA CTGGAGTGCTCACTCAAACGCTGGACTATGAATCATATAAGTTTAACTGATGGCGTCTAGGCTGCCGTGCATGTCGACTCCTGCGGTGCGGGGCTGGCTG TCTGGCTGGCGAGGAGCTGCTGCGCTTCCTTCACATGCTCTTGTTTTCCAGCTGCTTTCCTGGGGGATCAGACTGTGAAGCAGGAAGACAGATATAATAA ATATACTGCATCTTTTTAAGATGTGCAATTTTATTCTGAGGAAACATAAATTATGTTTTGTATTATATGACTTTAAGAGCCCACATTAGGTTTTATGATT CATTTGCCAGGTTTTTAAATGTTTTCACAAAACTGTTACGGGACTTCAACTAGAAATAAAATGGTGTAAATAAAGACCTTGCTATCTCTAAATTATGGAT GTTAAAGATTTGAAATGTTTTGTACTTTGATTATTTTTATTTCTTATACTCTGTTTTCTTTTATATTGATATCTTGCCCACATTTTAAATAAATGTACTT TTGAACTTAGAA

PAXIP1.F1 (forward primer)
PAXIP1.R1 (reverse primer)
Alternative donor

Frame 0

VRGRNAS*LNILFRLIFKIFPS*KFSCTVVLPW V*HQWPPPWRMGIPSDQS*AWKGGDSYFADELGW CRRNCRRLSDLGLLEVRPCNNFLDPFCPVTLG* RDFKAGFTPVIPALWEAKAGGSPEVMSSRPAWP TW*SLISTKNTKISRAWWRAPVVSATREAEAGEL LEPGRWKLQ*AEIAPLHSSLGNNS*TPSQKKKK SLLSLNLQSKTVAEHGAMKMSACNSVSFFQY** FPRPSFFCLFFCFCFLLSTIFLFPLLPSLRVSF* DFGSYRGLEPNVT*NLEQVELEPYLLDFESNLS A*KCPWQTTFI*NSIFSEKF*AWTSDSLTLMTV DYKFTC*PSNMRQAWTRCQV*YRGEEDMVPAL*R PSLVGEGAHLDISQYKV*QISPLGCKVDTTEMS LSRCHFSWVLR*AEVKNMKEYFHVERMC*GMKA FMRLGYIRMITISGTE*YEINLLRYSVVWRLKRS YWFWHPQSS*KPSKVFLVECWWKGLDCRELKSE WKLRTERLDSSGSLS*EGNREGA*GREGWGGIC VASQARGRMWVTLLFTGGGNIAMDRETHEEGSRI RNRLEDRKRRGAFSEDQSNGCVKLKMLRGQGKE ER*SGPWPVTAVFSLTKY*ASALVHIY*WSVKY SEKVV*LVGDFIFF*GWSKSLISERRKLSAAERG PGRALPWLFSFSRLEKRRKFPTMH*PHT*SQRM GTIQRWEKLGKSLTYLL*SLLG*FCPFSVELFC Q*MVFLQNHVRFFLESLPAFLRCHLKTEVPCGLW LRSMGEIAS*PSIRNARI*LFQSQRGRNTNVL* SEQVLKL*LLTGFWIAYQRKPKRTKHFIILV*L FMKRKKRKRKRRRK*KMRNKILRMRVVQMRSQAL PALKKGLLQVTSSFHLNPTLKNLKGN*CLMILQ IHHRKNRREI*TGPRPKSHS*LQQNAGCLRERS LG*LTCVPMSHPSQVTFCPLRSGVI*WLLDKTSK VLKDQK**LPGVQLYGH*GILLIMLTFSR*TGH QM*HISYRLFQHLRKI*NSR*ITASRDIQMPMQ CCLAK*K*LQRHTCYSSSSRPSSSSSSTRFYTFS PSR*CSSSSSSSSRSLSNLTPSSRRIHFHSNSS SSSKPIRISFHSNSYSFHSNSCILHSSCIALSS SSSPFSSSMPCSSSSISCSSTSSSSSSLPSSSSS TACSSSSSNSRFSSSSSSACTSSSSSSRCKVRQ RHT*VRRHRRCSIRFHLSSPRSSSSNSSHHHRL SSISFLDMIQQWRFQKKASYWDVCLQLRIIQSRC LISNCWPPGKG*SRHMAALLTPPSRVDARTFSV RVKSAARMHRQ*EKERDVLLHTG*TQS*RRRKW YRRTEPFTSQWPSHQEESHVHSILFL*LDLLIVT EMT*N*WLIWQVPNIRVIYAAATQSSSVKNQLV *SMKKPKSGGYPVSTPSGLATFFWETLRH*GRF SIVATRHSVCRIHLPLPSI*F*IF*MLGEFP*KC LQSC**V*DYLPN*NRMK*LMSSLLPKEPELKT YHLPLKS*LQN*PLLCFSLDSSLSRFNSILRSS TFLVERLRSLHRSAHTSLPAK*LAP*SS*RRFLS *ST**RQSGWKNASGVRSSLMSRTTFSEMLRQK YFSLSAWKNP*NGHTFLHSLRQNIFTSHLESAQ VFPL*RQS*SVQEERCYPSSHLSGSSWSTSRTRV CRK*F*YPVKMTFIYAENILPEA*MFTMQSSF* LECSLKRWTMNHISLTDGV*AAVHVDSCGAGLA VWLARSCCASFTCSCFPAAFLGDQTVKQEDRYNK YTASF*DVQFYSEET*IMFCII*L*EPTLGFMI HLPGF*MFSQNCYGTSTRNKMV*IKTLLSLNYG C*RFEMFCTLIIFISYTLFSFILISCPHFK*MYF *T*

Frame 1

SVVGMHLS*ISSSG*SSKYFLLKSLAVQLCCLG CDISGPHLGGWGSPVIRVRPGRAETHISQMNLD GVEGIVEG*VILAFLRFVLVTTFWTLFAL*L*GK ETSRPGLPL*SQHFGRPRRVDHLRS*VRDQPGQ HGEASSLLKIQKLAGRGGGRLWSQLLGRLRQEN CWNLGGGSCSEPRSRHCTPAWATTARLRLKKKKK AFYL*IYRVKQLLNTER*R*VPVTVLAFSSIDD SPAPLFFVCFFVFVFCYPPFFSSPSFLAFVFHF RTLALIGDLNQMLLKTWNKLNWNHIYSTLNQI*V LKNVLGKPLSSETVYFLKSFEPGHLIL*LL*LW IISSHVDHLI*GRHGLGARYNTEVKRTWYLPFS APHWWGREHTWTYHSTRCSRFLHWGAK*TPLKCH SVDVISVGF*DKQKLRT*RNIFMWSVCAEE*RH SCVWDTSG*SLYLEQSDMR*IY*DTQ*CGD*RE AIGFGILKVRENRVKCFW*SVGGRGWIAGS*RVN GN*EQKG*TLQEVCPEKEIEKVPEEERAGEGSV LHHKRVEGCG*LYCLQVEGT*LWTGKLMKKGVG SETDWRIGKGGVPFLKTSRMDA*S*KCLEVKGRK KGKVAHGQ*PLFFHLLNIEPLL*FISISGR*SI LKRLSSLWVTLFFFEVGAKV**VKEESSLLQRG DLEERCPGYSASQGWKSEGSFLQCTSLTHNLRGW GQSRGGRSSGSL*LTCCKAFLGDSVRSVWNSSA SKWFFSRIMSDFFWNHCLPFSGVI*RQKCPVGF GYVLWGRLPANPQ*EMHAFDCSRAKGGEIRMCFK ASKY*NCDS*LGSGLRIRENQKGRSILSSSSDY L*RGRRGRGRGGGSRK*GTRFSE*G*YR*EVKP CQLSRRVSFR*PAVFT*IQH*KI*RGINV**FFR FITGKTGEKFKLDPGRSPTVSCSKTQAASGKGA WVD*LVCQCPTRPR*HFAP*GPG*FNGCWTKPP KF*KIRNDSYLESSCTDTEEYY**C*HSADEPAI KCSTYLTDSFSTYEKFRTAGESQPAGTYKCQCS AV*PSESDSRDTHATAAAAGPAAAAAAPGFTPS APADNAAPAAAAAADLSATLPPAAAASIFTATAA AAASPSASVFTATATVSTATVASSTAAASPSAA APALSAAACPAAAVPSAAAAPAPAAAACPAPAA AQPAPAAAATADSAAAAPAHAPAAAAAADAKSDS ATLESDVTGAAASGSTSAAPAAAAATAATTIAS AASAFWT*SSSGDSRRRLLIGMCVCNCGLSRAD V**ATAGHLEKDNPGTWRHC*PHLHESMHAPSL* ESSQQRVCTGNKRKKEMCYCTLVKHSLKEEENG TAAPSPSLPSGLPTRRKAMFTAYYFCDWIC*** QR*PKINGLFGRCQIYGLSMPQQHSPHL*RTNWF KV*KSQRVEDTLCQRPVAWRHSSGKL*GTEADS V*SLHGIQSAGSICPYPAFSFKSFRCLESSLKS VCRVVDEYKTTSQTETE*SS*CPAFFQKSQN*RR TTSH*KANSRIDPFCAFHWIRACPGSTVY*EAL HSWWRGCGVCTEVHTPHCQQSDSHREVPDGDFC REAHSDARVAGRMLQVSEVH**AELHSPRC*GRS TFLFQLGRILKTGTRFSTL*GKIFLHHTWNLPK SFHYEGNRRVCRRKGVIQAAIFPEAHGAQAELE FVGNNFNIL*K*PSFMPRIFCQRHRCSQCRVRSD WSAHSNAGL*II*V*LMASRLPCMSTPAVRGWL SGWRGAAALPSHALVFQLLSWGIRL*SRKTDII NILHLFKMCNFILRKHKLCFVLYDFKSPH*VL*F ICQVFKCFHKTVTGLQLEIKWCK*RPCYL*IMD VKDLKCFVL*LFLFLILCFLLY*YLAHILNKCT FELR

Frame 2

PW*ECILVEYPLQVNLQNISFLKV*LYSCVALG VTSVAPTLEDGDPQ*SELGLEGRRLIFRR*TWM V*KEL*KAE*SWPS*GSSL*QLFGPFLPCNFRV KRLQGRVYPCNPSTLGGQGGWIT*GHEFETSLAN MVKPHLY*KYKN*PGVVAGACGLSYSGG*GRRI AGTWEVEVAVSRDRATALQPGQQQLDSVSKKKK KPSIFEFTE*NSC*TRSDEDECL*QC*LFPVLMI PPPLFFLFVFLFLFFAIHHFSLPPPS*PSCFIL GLWLL*GT*TKCYLKPGTS*TGTIFTRL*IKSK CLKMSLANHFHLKQYIF*KVLSLDI*FSDSYDCG L*VHMLTI*YEAGMD*VPGIIQR*RGHGTCPLA PLIGGGGSTPGHITVQGVADFSTGVQSRHH*NV TQ*MSFQLGFEISRS*EHEGIFSCGAYVLRNEGI HAFGIHQDDHYIWNRVI*DKSIKILSSVEIEEK LLVLASSKFVKTE*SVFGRVLVEGAGLQGVEE* METENRKVRLFRKFVLRRK*RRCLRKRGLGRDLC CITSAWKDVGDFTVYRWREHSYGQGNS*RRE*D QKQTGG*EKEGCLF*RPVEWMREAKNA*RSREG RKVKWPMASDRCFFTY*ILSLCSSSYLLVVGKVF *KGCLACG*LYFFLRLEQKFNK*KKKALCCREG TWKSVALVIQLLKAGKAKEVSYNALASHIISED GDNPEVGEAREVFDLPVVKPSWVILSVQCGTLLP VNGFSPESCQIFFGITACLSQVSSEDRSALWAL VTFYGGDCQLTLNKKCTHLIVPEPKGEKYECAL KRASIKIVTPDWVLDCVSEKTKKDEAFYHPRLII YEEEEEEEEEEEEVENEEQDSQNEGSTDEKSSP ASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSS DSSPEKQERNLNWTPAEVPQLAAAKRRLPQGKEP GLINLCANVPPVPGNILPPEVRGNLMAAGQNLQ SSERSEMIATWSPAVRTLRNITNNADIQQMNRP SNVAHILQTLSAPTKNLEQQVNHSQQGHTNANAV LFSQVKVTPETHMLQQQQQAQQQQQQHPVLHLQ PQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQ QQQQAHPHQFSQQQLQFPQQQLHPPQQLHRPQQQ LQPFQQQHALQQQFHQLQQHQLQQQQLAQLQQQ HSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQT APHLSQTSQALQHQVPPQQPPQQQQQQQPPPSPQ QHQLFGHDPAVEIPEEGFLLGCVFAIADYPEQM SDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLC ESQVSSAYAQAIRERKRCVTAHWLNTVLKKKKMV PPHRALHFPVAFPPGGKPCSQHIISVTGFVDSD RDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTG LKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQ YSRYTAFSLQDPFAPTQHLVLNLLDAWRVPLKV SAELLMSIRLPPKLKQNEVANVQPSSKRARIED VPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLY ILGGEVAESAQKCTHLIASKVTRTVKFLTAISV VKHIVTPEWLEECFRCQKFIDEQNYILRDAEAE VLFSFSLEESLKRAHVSPLFKAKYFYITPGICPS LSTMKAIVECAGGKVLSKQPSFRKLMEHKQNSS LSEIILISCENDLHLCREYFARGIDVHNAEFVL TGVLTQTLDYESYKFN*WRLGCRACRLLRCGAGC LAGEELLRFLHMLLFSSCFPGGSDCEAGRQI** IYCIFLRCAILF*GNINYVLYYMTLRAHIRFYD SFARFLNVFTKLLRDFN*K*NGVNKDLAISKLWM LKI*NVLYFDYFYFLYSVFFYIDILPTF*INVL LNLE

Sequence PAXIP1.bAug05

GCCGCGGAGCCTCCCGGGCCGCCGCGATCATGTCGGACCAGGCGCCCAAAGTTCCTGAGGAGATGTTCAGGGAGGTCAAGTATTACGCGGTGGGCGACAT CGACCCGCAGGTTATTCAGCTTCTCAAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACATAATCTCAGAGGATGGGGACAATCCA GAGGTGGGAGAAGCTCGGGAAGTCTTTGACTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGTGGAACTCTTCTGCCAGTAAATGGTT TTTCTCCAGAATCATGTCAGATTTTTTTTGGAATCACTGCCTGCCTTTCTCAGGGTGTTGATACAAGCTGGAGCTCTTTGTTGGAGTCTTCCAGAGCTCT CCCAGGGAGAGGTAGGGAAGGGAGCTTGTCCAGCAGAAGTTGGGAAGCACAGAGATCATCTGCCTTCTTCTGACCCGGTATTGATGCAGGCTGAGGCCTC TGTTGTAATGTGCTGGGTGTCATCTGAAGACAGAAGTGCCCTGTGGGCTTTGGTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTCAATAAGAAATGC ACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATACGAATGTGCTTTAAAGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGGTTCTGGATTGCG TATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCATCCTCGTCTGATTATTTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGAGGAAGTAGAAAA TGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATGAGAAGTCAAGCCCTGCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAGCAGTTTTCACCT AAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTTTGATGATTCTTCAGATTCATCACCGGAAAAACAGGAGAGAAATTTAAACTGGACCCCGGCCG AAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCTCAGGGAAAGGAGCCTGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGTCCCAGGTAACAT TTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTGGACAAAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGTCCAGCTGTACGG ACACTGAGGAATATTACTAATAATGCTGACATTCAGCAGATGAACCGGCCATCAAATGTAGCACATATCTTACAGACTCTTTCAGCACCTACGAAAAATT TAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACAAATGCCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACACATGCTACAGCA GCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGGTTTTACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAGCAGCAGATCTCT CAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTCACAGCAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGCAACAGCTACAGT TTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCATCGCCCTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCAGCAGTTCCATCA GCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTTGCCCAGCTCCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATTCAGCAGCAGCAG CTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGATGCAAAGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGCATCAGGTTCCAC CTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCACCACCATCGCCTCAGCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGAGATTCCAGAAGA AGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCCAGGCACAT GGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGA GATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGAAGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGG AAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTGTTGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACG GGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAAGAACCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTGTGTCAACGCCC AGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCACTGAGGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCATTTGCCCCTAC CCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGTTCCCTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCAAACTGAAACAG AATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGAATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGC TTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATTAAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCT CATTGCCAGCAAAGTGACTCGCACCGTGAAGTTCCTGACGGCGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGT CAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGATGCTGAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTT CTCCACTCTTTAAGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGGAGGAAAGGTGTT ATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGACCTTCATTTATGC CGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCATATAAGTTTAACT GATGGCGTCTAGGCTGCCGTGCATGTCGACTCCTGCGGTGCGGGGCTGGCTGTCTGGCTGGCGAGGAGCTGCTGCGCTTCCTTCACATGCTCTTGTTTTC CAGCTGCTTTCCTGGGGGATCAGACTGTGAAGCAGGAAGACAGATATAATAAATATACTGCATCTTTTTAAGATGTGCAATTTTATTCTGAGGAAACATA AATTATGTTTTGTATTATATGACTTTAAGAGCCCACATTAGGTTTTATGATTCATTTGCCAGGTTTTTAAATGTTTTCACAAAACTGTTACGGGACTTCA ACTAGAAATAAAATGGTGTAAATAAAGACCTTGCTATCTCTAAATTATGGATGTTAAAGATTTGAAATGTTTTGTACTTTGATTATTTTTATTTCTTATA CTCTGTTTTCTTTTATATTGATATCTTGCCCACATTTTAAATAAATGTACTTTTGAACTTAGAA

PAXIP1.F1 (forward primer)
PAXIP1.R1 (reverse primer)
Alternative donor

Frame 0

AAEPPGPPRSCRTRRPKFLRRCSGRSSITRWAT STRRLFSFSRLEKRRKFPTMH*PHT*SQRMGTIQ RWEKLGKSLTYLL*SLLG*FCPFSVELFCQ*MV FLQNHVRFFLESLPAFLRVLIQAGALCWSLPEL SQGEVGKGACPAEVGKHRDHLPSSDPVLMQAEAS VVMCWVSSEDRSALWALVTFYGGDCQLTLNKKC THLIVPEPKGEKYECALKRASIKIVTPDWVLDC VSEKTKKDEAFYHPRLIIYEEEEEEEEEEEEVEN EEQDSQNEGSTDEKSSPASSQEGSPSGDQQFSP KSNTEKSKGELMFDDSSDSSPEKQERNLNWTPA EVPQLAAAKRRLPQGKEPGLINLCANVPPVPGNI LPPEVRGNLMAAGQNLQSSERSEMIATWSPAVR TLRNITNNADIQQMNRPSNVAHILQTLSAPTKN LEQQVNHSQQGHTNANAVLFSQVKVTPETHMLQQ QQQAQQQQQQHPVLHLQPQQIMQLQQQQQQQIS QQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQLQ FPQQQLHPPQQLHRPQQQLQPFQQQHALQQQFHQ LQQHQLQQQQLAQLQQQHSLLQQQQQQQIQQQQ LQRMHQQQQQQQMQSQTAPHLSQTSQALQHQVP PQQPPQQQQQQQPPPSPQQHQLFGHDPAVEIPEE GFLLGCVFAIADYPEQMSDKQLLATWKRIIQAH GGTVDPTFTSRCTHLLCESQVSSAYAQAIRERK RCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGG KPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYT GYLCRSNTVLICKEPTGLKYEKAKEWRIPCVNA QWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPT QHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQ NEVANVQPSSKRARIEDVPPPTKKLTPELTPFV LFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHL IASKVTRTVKFLTAISVVKHIVTPEWLEECFRC QKFIDEQNYILRDAEAEVLFSFSLEESLKRAHV SPLFKAKYFYITPGICPSLSTMKAIVECAGGKVL SKQPSFRKLMEHKQNSSLSEIILISCENDLHLC REYFARGIDVHNAEFVLTGVLTQTLDYESYKFN *WRLGCRACRLLRCGAGCLAGEELLRFLHMLLFS SCFPGGSDCEAGRQI**IYCIFLRCAILF*GNI NYVLYYMTLRAHIRFYDSFARFLNVFTKLLRDF N*K*NGVNKDLAISKLWMLKI*NVLYFDYFYFLY SVFFYIDILPTF*INVLLNLE

Frame 1

PRSLPGRRDHVGPGAQSS*GDVQGGQVLRGGRH RPAGYSASQGWKSEGSFLQCTSLTHNLRGWGQS RGGRSSGSL*LTCCKAFLGDSVRSVWNSSASKWF FSRIMSDFFWNHCLPFSGC*YKLELFVGVFQSS PRER*GRELVQQKLGSTEIICLLLTRY*CRLRP LL*CAGCHLKTEVPCGLWLRSMGEIAS*PSIRNA RI*LFQSQRGRNTNVL*SEQVLKL*LLTGFWIA YQRKPKRTKHFIILV*LFMKRKKRKRKRRRK*K MRNKILRMRVVQMRSQALPALKKGLLQVTSSFHL NPTLKNLKGN*CLMILQIHHRKNRREI*TGPRP KSHS*LQQNAGCLRERSLG*LTCVPMSHPSQVT FCPLRSGVI*WLLDKTSKVLKDQK**LPGVQLYG H*GILLIMLTFSR*TGHQM*HISYRLFQHLRKI *NSR*ITASRDIQMPMQCCLAK*K*LQRHTCYS SSSRPSSSSSSTRFYTFSPSR*CSSSSSSSSRSL SNLTPSSRRIHFHSNSSSSSKPIRISFHSNSYS FHSNSCILHSSCIALSSSSSPFSSSMPCSSSSI SCSSTSSSSSSLPSSSSSTACSSSSSNSRFSSSS SSACTSSSSSSRCKVRQRHT*VRRHRRCSIRFH LSSPRSSSSNSSHHHRLSSISFLDMIQQWRFQK KASYWDVCLQLRIIQSRCLISNCWPPGKG*SRHM AALLTPPSRVDARTFSVRVKSAARMHRQ*EKER DVLLHTG*TQS*RRRKWYRRTEPFTSQWPSHQE ESHVHSILFL*LDLLIVTEMT*N*WLIWQVPNIR VIYAAATQSSSVKNQLV*SMKKPKSGGYPVSTP SGLATFFWETLRH*GRFSIVATRHSVCRIHLPL PSI*F*IF*MLGEFP*KCLQSC**V*DYLPN*NR MK*LMSSLLPKEPELKTYHLPLKS*LQN*PLLC FSLDSSLSRFNSILRSSTFLVERLRSLHRSAHT SLPAK*LAP*SS*RRFLS*ST**RQSGWKNASGV RSSLMSRTTFSEMLRQKYFSLSAWKNP*NGHTF LHSLRQNIFTSHLESAQVFPL*RQS*SVQEERC YPSSHLSGSSWSTSRTRVCRK*F*YPVKMTFIYA ENILPEA*MFTMQSSF*LECSLKRWTMNHISLT DGV*AAVHVDSCGAGLAVWLARSCCASFTCSCF PAAFLGDQTVKQEDRYNKYTASF*DVQFYSEET* IMFCII*L*EPTLGFMIHLPGF*MFSQNCYGTS TRNKMV*IKTLLSLNYGC*RFEMFCTLIIFISY TLFSFILISCPHFK*MYF*T*

Frame 2

RGASRAAAIMSDQAPKVPEEMFREVKYYAVGDI DPQVIQLLKAGKAKEVSYNALASHIISEDGDNP EVGEAREVFDLPVVKPSWVILSVQCGTLLPVNG FSPESCQIFFGITACLSQGVDTSWSSLLESSRAL PGRGREGSLSSRSWEAQRSSAFF*PGIDAG*GL CCNVLGVI*RQKCPVGFGYVLWGRLPANPQ*EM HAFDCSRAKGGEIRMCFKASKY*NCDS*LGSGLR IRENQKGRSILSSSSDYL*RGRRGRGRGGGSRK *GTRFSE*G*YR*EVKPCQLSRRVSFR*PAVFT *IQH*KI*RGINV**FFRFITGKTGEKFKLDPGR SPTVSCSKTQAASGKGAWVD*LVCQCPTRPR*H FAP*GPG*FNGCWTKPPKF*KIRNDSYLESSCT DTEEYY**C*HSADEPAIKCSTYLTDSFSTYEKF RTAGESQPAGTYKCQCSAV*PSESDSRDTHATA AAAGPAAAAAAPGFTPSAPADNAAPAAAAAADL SATLPPAAAASIFTATAAAAASPSASVFTATATV STATVASSTAAASPSAAAPALSAAACPAAAVPS AAAAPAPAAAACPAPAAAQPAPAAAATADSAAA APAHAPAAAAAADAKSDSATLESDVTGAAASGST SAAPAAAAATAATTIASAASAFWT*SSSGDSRR RLLIGMCVCNCGLSRADV**ATAGHLEKDNPGT WRHC*PHLHESMHAPSL*ESSQQRVCTGNKRKKE MCYCTLVKHSLKEEENGTAAPSPSLPSGLPTRR KAMFTAYYFCDWIC***QR*PKINGLFGRCQIY GLSMPQQHSPHL*RTNWFKV*KSQRVEDTLCQRP VAWRHSSGKL*GTEADSV*SLHGIQSAGSICPY PAFSFKSFRCLESSLKSVCRVVDEYKTTSQTET E*SS*CPAFFQKSQN*RRTTSH*KANSRIDPFCA FHWIRACPGSTVY*EALHSWWRGCGVCTEVHTP HCQQSDSHREVPDGDFCREAHSDARVAGRMLQV SEVH**AELHSPRC*GRSTFLFQLGRILKTGTRF STL*GKIFLHHTWNLPKSFHYEGNRRVCRRKGV IQAAIFPEAHGAQAELEFVGNNFNIL*K*PSFM PRIFCQRHRCSQCRVRSDWSAHSNAGL*II*V*L MASRLPCMSTPAVRGWLSGWRGAAALPSHALVF QLLSWGIRL*SRKTDIINILHLFKMCNFILRKH KLCFVLYDFKSPH*VL*FICQVFKCFHKTVTGLQ LEIKWCK*RPCYL*IMDVKDLKCFVL*LFLFLI LCFLLY*YLAHILNKCTFELR

Sequence PAXIP1.dAug05

CAATAAAGTAAGCTAAAGAAAAGAAAATGTTATTAAGAAAATCATAAGGAAGAGAAAATATATGTACTATTCATTAAGGGGAAGTATATCATCATAAAGG TCTTCATCCTCGTCGTTTTCACGATGAGTGGGCCTAGGAGGAGGAGGGACAGGAGGGGCTGGTCTTACTCCCTCAGGGGTGGCAGAGGTGGAAGAAAATC CAGCTATCAGTGGACCCACACAGTTCACACCTGTGTTGCTTAAGTATAGGTGATACTACATTCATTTTCTCTTGCTGTGTAACAGATTATCTCAAAACCC AGTGGCTTAAAACAATAAACATTACCTCTCAGTTTGGTCGTGGGTCAGAAATCCTGCAACAGCTTGGCAGGGCGGTTGTGGCTCAGCTATCTCCTGAGGT TGCAGTCAGGATGCCAGCGGAGGCTGCAGGAGCCCCTTCCAGGATGGTTCCGTCACATGGCTGTGGGCTGGAGGCCTCAGCTCCTCACCACAGAGGCTCT CCAGAGGGCTGGTTGTGTGTCTTCACAGCATGACAGCTGGCTCCCTTCCCCAGGGGGTGTGACCCAAGATAGAGGGAAAACCCGCAGCGTTATGGTGTCT TTTGTGACTGAGTTATGGGAGACTCCCACTGTCAATTCCACCATATTAAGTGACTAAGTCTAGACCATATTTAAGGCTAGGGGAGTAAAGCTCCAACTCT TAGAGGGAGGAGTATACAGTAATTTGCAGACATATTATAGAACCACCATATGTACATTTTTCATAGAGTTTGGGAATGAAATAGTAAAAGGTATATAGAA AACTAAGAAAAGGGAAAATTCTGGGGGGCTGGGATGAGGAAGTGATTAGCACCAGGGAAAACCAAAGTTTATACCAGAAAGGAACTCTAATCTTAGGCTG CCATATTAAGCCATGTGGCTGGGCTACATTGTGTTAAGTCACTGATGAATGATCTAAACAAGAGTCTGGATATAACCAAATCAGGAAGGCTTGAAGAATG TGTGTGTTATTGGGGGGAACGGTGTCCACAGTAGGAGGTAGATGGTTATGTAAAACTAGAGAAAAGGAACTAATATAAAGTGTTAGTTGGAATATATTAA TAATTGTCAGTGGAAACAGCTTAAAGTGCTAAATGTGCTTCAGGGGAGTGAAAAGCAGGGATGGGTGAGGAACCTGTGGTTTTGTCCTGTGCCTCATTAA ACTATATTTATGCTTAGCCATAGGAAAAATAAACATTCACATCTTAAAAGTAGTAAGTGAAAGAATCGGTTTTCTTAGGGGGCAGGTCTTTTTAAAGTTT ATATTTCACCTAATAGGCCGTCAGATTCTCCTTTTTCCATGTACAATATTTTGAGTTCTGAAGAATGTCCAGGGTCGTGCAGCTGTCACAGCCCGGACAC AGAGCAATTCCTTCCCCCAGAAAACCAGATGTGTTTTGAGAAGAAACTGTTGGGATTTACTAATTTCATTCTGATTTGCTTTTTATTTCCCTTTTTAAGG GAGGTGGATCTGCCCACCATAGACCTTTGGCAGTATCTGAAGATCTTTAGTAGTCGCGGCTAGGGGGCTGCTGCTGGCATAGGGTGGGTAGAAGTTGGGG ACACTGCCAAACATCCTCCTCTGTACAGGACAGCCCCCACTTCCCAGAACTCCCTGGTCCAGAGTGGCAGTTACATTGAGCTTGAGAAGCCCTGGAGTGC ATTCCATTGTTCTTCTCTTGTATCCTGGGAATTCAGATTAAATTTAGAGTATTGGAGTTCATTTTCATTGTATGGTGCTGGGTAGAAAAGTCCGTAAATA GAACTGTTACATGTCCTTGATACTTTGAGGAAATACATGATATAAATATATGATAGAGTCTTTTAATAGCTGCCTTATTTGAAATTGGAATTCTCCTCTA TTCTGAGTGATTGATGACTGCATTGGTCGGGTCTTTGTGTGTAGATAATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCA CCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAG AAGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTG TTGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAAGA ACCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTGTGTCAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCACTG AGGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCATTTGCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGTTC CCTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCAAACTGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCCAG AATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATT AAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCTCATTGCCAGCAAAGTGACTCGCACCGTGAAGTTCCTGACGG CGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGATGC TGAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTTCTCCACTCTTTAAGGTACACTTTAAGGGAAAAGTAAATCAG TCCAAGTAAGCATAACACACGGACCTGCTCAAGGGAACTACCAGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAGTCTTTCCACTATGAAGGC AATCGTAGAGTGTGCAGGAGGAAAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGTTTGTCGGAAATAATTTTA ATATCCTGTGAAAATGACCTTCATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGACTGGAGTGCTCACTCAAA CGCTGGACTATGAATCATATAAGTTTAACTGATGGCGTCTAGGCTGCCGTGCATGTCGACTCCTGCGGTGCGGGGCTGGCTGTCTGGCTGGCGAGGAGCT GCTGCGCTTCCTTCACATGCTCTTGTTTTCCAGCTGCTTTCCTGGGGGATCAGACTGTGAAGCAGGAAGACAGATATAATAAATATACTGCATCTTTTTA AGATGTGCAATTTTATTCTGAGGAAACATAAATTATGTTTTGTATTATATGACTTTAAGAGCCCACATTAGGTTTTATGATTCATTTGCCAGGTTTTTAA ATGTTTTCACAAAACTGTTACGGGACTTCAACTAGAAATAAAATGGTGTAAATAAAGACCTTGCTATCTCTAAATTATGGATGTTAAAGATTTGAAATGT TTTGTACTTTGATTATTTTTATTTCTTATACTCTGTTTTCTTTTATATTGATATCTTGCCCACATTTTAAATAAATGTACTTTTGAACTTAGAA

PAXIP1.F1 (forward primer)
PAXIP1.R1 (reverse primer)
Alternative donor

Frame 0

Q*SKLKKRKCY*ENHKEEKIYVLFIKGKYIIIK VFILVVFTMSGPRRRRDRRGWSYSLRGGRGGRKS SYQWTHTVHTCVA*V*VILHSFSLAV*QIISKP SGLKQ*TLPLSLVVGQKSCNSLAGRLWLSYLLR LQSGCQRRLQEPLPGWFRHMAVGWRPQLLTTEAL QRAGCVSSQHDSWLPSPGGVTQDRGKTRSVMVS FVTELWETPTVNSTILSD*V*TIFKARGVKLQL LEGGVYSNLQTYYRTTICTFFIEFGNEIVKGI*K TKKRENSGGLG*GSD*HQGKPKFIPERNSNLRL PY*AMWLGYIVLSH**MI*TRVWI*PNQEGLKN VCVIGGNGVHSRR*MVM*N*RKGTNIKC*LEYIN NCQWKQLKVLNVLQGSEKQGWVRNLWFCPVPH* TIFMLSHRKNKHSHLKSSK*KNRFS*GAGLFKV YISPNRPSDSPFSMYNILSSEECPGSCSCHSPDT EQFLPPENQMCFEKKLLGFTNFILICFLFPFLR EVDLPTIDLWQYLKIFSSRG*GAAAGIGWVEVG DTAKHPPLYRTAPTSQNSLVQSGSYIELEKPWSA FHCSSLVSWEFRLNLEYWSSFSLYGAG*KSP*I ELLHVLDTLRKYMI*IYDRVF**LPYLKLEFSS ILSD**LHWSGLCV*IIQAHGGTVDPTFTSRCTH LLCESQVSSAYAQAIRERKRCVTAHWLNTVLKK KKMVPPHRALHFPVAFPPGGKPCSQHIISVTGF VDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKE PTGLKYEKAKEWRIPCVNAQWLGDILLGNFEAL RQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRV PLKVSAELLMSIRLPPKLKQNEVANVQPSSKRAR IEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYI KKLYILGGEVAESAQKCTHLIASKVTRTVKFLT AISVVKHIVTPEWLEECFRCQKFIDEQNYILRDA EAEVLFSFSLEESLKRAHVSPLFKVHFKGKVNQ SK*A*HTDLLKGTTRQNIFTSHLESAQVFPL*R QS*SVQEERCYPSSHLSGSSWSTSRTRVCRK*F* YPVKMTFIYAENILPEA*MFTMQSSF*LECSLK RWTMNHISLTDGV*AAVHVDSCGAGLAVWLARS CCASFTCSCFPAAFLGDQTVKQEDRYNKYTASF* DVQFYSEET*IMFCII*L*EPTLGFMIHLPGF* MFSQNCYGTSTRNKMV*IKTLLSLNYGC*RFEM FCTLIIFISYTLFSFILISCPHFK*MYF*T*

Frame 1

NKVS*RKENVIKKIIRKRKYMYYSLRGSISS*R SSSSSFSR*VGLGGGGTGGAGLTPSGVAEVEEN PAISGPTQFTPVLLKYR*YYIHFLLLCNRLSQNP VA*NNKHYLSVWSWVRNPATAWQGGCGSAIS*G CSQDASGGCRSPFQDGSVTWLWAGGLSSSPQRL SRGLVVCLHSMTAGSLPQGV*PKIEGKPAALWCL L*LSYGRLPLSIPPY*VTKSRPYLRLGE*SSNS *REEYTVICRHIIEPPYVHFS*SLGMK**KVYR KLRKGKILGGWDEEVISTRENQSLYQKGTLILGC HIKPCGWATLC*VTDE*SKQESGYNQIRKA*RM CVLLGGTVSTVGGRWLCKTREKELI*SVSWNIL IIVSGNSLKC*MCFRGVKSRDG*GTCGFVLCLIK LYLCLAIGKINIHILKVVSERIGFLRGQVFLKF IFHLIGRQILLFPCTIF*VLKNVQGRAAVTART QSNSFPQKTRCVLRRNCWDLLISF*FAFYFPF*G RWICPP*TFGSI*RSLVVAARGLLLA*GG*KLG TLPNILLCTGQPPLPRTPWSRVAVTLSLRSPGV HSIVLLLYPGNSD*I*SIGVHFHCMVLGRKVRK* NCYMSLIL*GNT*YKYMIESFNSCLI*NWNSPL F*VIDDCIGRVFVCR*SRHMAALLTPPSRVDAR TFSVRVKSAARMHRQ*EKERDVLLHTG*TQS*RR RKWYRRTEPFTSQWPSHQEESHVHSILFL*LDL LIVTEMT*N*WLIWQVPNIRVIYAAATQSSSVK NQLV*SMKKPKSGGYPVSTPSGLATFFWETLRH* GRFSIVATRHSVCRIHLPLPSI*F*IF*MLGEF P*KCLQSC**V*DYLPN*NRMK*LMSSLLPKEP ELKTYHLPLKS*LQN*PLLCFSLDSSLSRFNSIL RSSTFLVERLRSLHRSAHTSLPAK*LAP*SS*R RFLS*ST**RQSGWKNASGVRSSLMSRTTFSEM LRQKYFSLSAWKNP*NGHTFLHSLRYTLREK*IS PSKHNTRTCSRELPGKIFLHHTWNLPKSFHYEG NRRVCRRKGVIQAAIFPEAHGAQAELEFVGNNF NIL*K*PSFMPRIFCQRHRCSQCRVRSDWSAHSN AGL*II*V*LMASRLPCMSTPAVRGWLSGWRGA AALPSHALVFQLLSWGIRL*SRKTDIINILHLF KMCNFILRKHKLCFVLYDFKSPH*VL*FICQVFK CFHKTVTGLQLEIKWCK*RPCYL*IMDVKDLKC FVL*LFLFLILCFLLY*YLAHILNKCTFELR

Frame 2

IK*AKEKKMLLRKS*GRENICTIH*GEVYHHKG LHPRRFHDEWA*EEEGQEGLVLLPQGWQRWKKI QLSVDPHSSHLCCLSIGDTTFIFSCCVTDYLKT QWLKTINITSQFGRGSEILQQLGRAVVAQLSPEV AVRMPAEAAGAPSRMVPSHGCGLEASAPHHRGS PEGWLCVFTA*QLAPFPRGCDPR*RENPQRYGV FCD*VMGDSHCQFHHIK*LSLDHI*G*GSKAPTL RGRSIQ*FADIL*NHHMYIFHRVWE*NSKRYIE N*EKGKFWGAGMRK*LAPGKTKVYTRKEL*S*A AILSHVAGLHCVKSLMNDLNKSLDITKSGRLEEC VCYWGERCPQ*EVDGYVKLEKRN*YKVLVGIY* *LSVETA*SAKCASGE*KAGMGEEPVVLSCASL NYIYA*P*EK*TFTS*K**VKESVFLGGRSF*SL YFT**AVRFSFFHVQYFEF*RMSRVVQLSQPGH RAIPSPRKPDVF*EETVGIY*FHSDLLFISLFK GGGSAHHRPLAVSEDL**SRLGGCCWHRVGRSWG HCQTSSSVQDSPHFPELPGPEWQLH*A*EALEC IPLFFSCILGIQIKFRVLEFIFIVWCWVEKSVN RTVTCP*YFEEIHDINI**SLLIAALFEIGILLY SE*LMTALVGSLCVDNPGTWRHC*PHLHESMHA PSL*ESSQQRVCTGNKRKKEMCYCTLVKHSLKE EENGTAAPSPSLPSGLPTRRKAMFTAYYFCDWIC ***QR*PKINGLFGRCQIYGLSMPQQHSPHL*R TNWFKV*KSQRVEDTLCQRPVAWRHSSGKL*GT EADSV*SLHGIQSAGSICPYPAFSFKSFRCLESS LKSVCRVVDEYKTTSQTETE*SS*CPAFFQKSQ N*RRTTSH*KANSRIDPFCAFHWIRACPGSTVY *EALHSWWRGCGVCTEVHTPHCQQSDSHREVPDG DFCREAHSDARVAGRMLQVSEVH**AELHSPRC *GRSTFLFQLGRILKTGTRFSTL*GTL*GKSKS VQVSITHGPAQGNYQAKYFYITPGICPSLSTMKA IVECAGGKVLSKQPSFRKLMEHKQNSSLSEIIL ISCENDLHLCREYFARGIDVHNAEFVLTGVLTQ TLDYESYKFN*WRLGCRACRLLRCGAGCLAGEEL LRFLHMLLFSSCFPGGSDCEAGRQI**IYCIFL RCAILF*GNINYVLYYMTLRAHIRFYDSFARFL NVFTKLLRDFN*K*NGVNKDLAISKLWMLKI*NV LYFDYFYFLYSVFFYIDILPTF*INVLLNLE