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Sequences

Sequence STIM1.aAug05

TGGAAGCCGCTGTCCTGGGCCTGGCCGGTGTGCGTCCGCCTGCTGGACCTGGGCACCGCCAGCCGCCTGGGCACGGGACTGGGCGGGGGCGCTGACCTCG GCCTAGGAGGCCCAGGATCCCGGAGACGCCCGCGCCCTCAGGACCCTGCGGGTCGCACGCCCTCCCCAGCTTCTGCTGCTCGCCGCTCTTCGGCAGGGCG AGGTCAGGTGCCCCCTTCTCGCCTCTCTTCTCTTCTCTTCTCTTCCTCCTCCACTTCTGTGCCCGCGGAGACTCCGGCCGCCCCCTTCCGCAGGGGTGTA GTAATCTGCGGAGCTGACAGCAGCCCCGCAGCCACCCTGCCCGAAGTCTCCGGAAGCGGCACGAGCTCAGGCCGCCGCAGCCCCGGCGGACCCACTGTTG GACCTGAGGAGCCAGCCCTCCTCCCGCACCCAAACTTGGAGCACTTGACCTTTGGCTGTTGGAGGGGGCAGGCTCGCGGGTGGCTGGACAGCTGCGGAGC CGCGAGGGCATCTTGCCTGGAGACCGTCGGCTGCACTCCCGGGCTCCTGGCTTTGCCTCTGGGATCCCGAGGTGTCCACATCAGACGCATGTTGACTGAG ACCTAGAGTCATGGATGTATGCGTCCGTCTTGCCCTGTGGCTCCTCTGGGGACTCCTCCTGCACCAGGGCCAGAGCCTCAGCCATAGTCACAGTGAGAAG GCGACAGGAACCAGCTCGGGGGCCAACTCTGAGGAGTCCACTGCAGCAGAGTTTTGCCGAATTGACAAGCCCCTGTGTCACAGTGAGGATGAGAAACTCA GCTTCGAGGCAGTCCGTAACATCCACAAACTGATGGACGATGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTGAGGGAAGACCTCAA TTACCATGACCCAACAGTGAAACACAGCACCTTCCATGGTGAGGATAAGCTCATCAGCGTGGAGGACCTGTGGAAGGCATGGAAGTCATCAGAAGTATAC AATTGGACCGTGGATGAGGTGGTACAGTGGCTGATCACATATGTGGAGCTGCCTCAGTATGAGGAGACCTTCCGGAAGCTGCAGCTCAGTGGCCATGCCA TGCCAAGGCTGGCTGTCACCAACACCACCATGACAGGGACTGTGCTGAAGATGACAGACCGGAGTCATCGGCAGAAGCTGCAGCTGAAGGCTCTGGATAC AGTGCTCTTTGGGCCTCCTCTCTTGACTCGCCATAATCACCTCAAGGACTTCATGCTGGTGGTGTCTATCGTTATTGGTGTGGGCGGCTGCTGGTTTGCC TATATCCAGAACCGTTACTCCAAGGAGCACATGAAGAAGATGATGAAGGACTTGGAGGGGTTACACCGAGCTGAGCAGAGTCTGCATGACCTTCAGGAAA GGCTGCACAAGGCCCAGGAGGAGCACCGCACAGTGGAGGTGGAGAAGGTCCATCTGGAAAAGAAGCTGCGCGATGAGATCAACCTTGCTAAGCAGGAAGC CCAGCGGCTGAAGGAGCTGCGGGAGGGTACTGAGAATGAGCGGAGCCGCCAAAAATATGCTGAGGAGGAGTTGGAGCAGGTTCGGGAGGCCTTGAGGAAA GCAGAGAAGGAGCTAGAATCTCACAGCTCATGGTATGCTCCAGAGGCCCTTCAGAAGTGGCTGCAGCTGACACATGAGGTGGAGGTGCAATATTACAACA TCAAGAAGCAAAATGCTGAGAAGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAGATAAAAAAGAAGAGAAACACACTCTTTGGCACCTTCCACGTGGC CCACAGCTCTTCCCTGGATGATGTAGATCATAAAATTCTAACAGCTAAGCAAGCACTGAGCGAGGTGACAGCAGCATTGCGGGAGCGCCTGCACCGCTGG CAACAGATCGAGATCCTCTGTGGCTTCCAGATTGTCAACAACCCTGGCATCCACTCACTGGTGGCTGCCCTCAACATAGACCCCAGCTGGATGGGCAGTA CACGCCCCAACCCTGCTCACTTCATCATGACTGACGACGTGGATGACATGGATGAGGAGATTGTGTCTCCCTTGTCCATGCAGTCCCCTAGCCTGCAGAG CAGTGTTCGGCAGCGCCTGACGGAGCCACAGCATGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACATGAGTGACCGC CAGCGTGTGGCCCCCAAACCTCCTCAGATGAGCCGTGCTGCAGACGAGGCTCTCAATGCCATGACTTCCAATGGCAGCCACCGGCTGATCGAGGGGGTCC ACCCAGGGTCTCTGGTGGAGAAACTGCCTGACAGCCCTGCCCTGGCCAAGAAGGCATTACTGGCGCTGAACCATGGGCTGGACAAGGCCCACAGCCTGAT GGAGCTGAGCCCCTCAGCCCCACCTGGTGGCTCTCCACATTTGGATTCTTCCCGTTCTCACAGCCCCAGCTCCCCAGACCCAGACACACCATCTCCAGTT GGGGACAGCCGAGCCCTGCAAGCCAGCCGAAACACACGCATTCCCCACCTGGCTGGCAAGAAGGCTGTGGCTGAGGAGGATAATGGCTCTATTGGCGAGG AAACAGACTCCAGCCCAGGCCGGAAGAAGTTTCCCCTCAAAATCTTTAAGAAGCCTCTTAAGAAGTAGGCAGGATGGGGTGGCAGTAAAGGGACAGCTTG TCCTTCCCTGGGTGTTCTGTCTCTCCTTCCCTCCCTTCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGAAGGGCTGGTCCAGGGGTCTGGGCACT GTACATACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTATTAACTGACCACCATGGCCTGCCTGCCCTGCCTCCGTCCCAACCATGGGCTGCTGC TGTCACTCCCTCTCCACTTCAGTGCATGTCTTAGTTGCTGTTCCCTCAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTCTCTGCTGTCCCAGTTTT GAGGTTTGGTTTCTTGTTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACCACTCCCCAACTTCCCCTAGCAGTTGCAGGGAAGATAGGACGAGTAGCT TCTGACATGTGTGCCTCAGATCTGTTCCACCCCACTCACAGTGGTTCTGTTTGCTCCAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTGTTCTTTGGTA TTGATGTGGGTCAGAAGGAGCCTCATCCTAATCTCACTCAGGCCTCCAGGGATCCATGGGGGAGTGAAACCAATTCTCAGAGAACAACCCACCAGAGACT TTTAAAGAGAGGCCAGGCTTGGGAATGGGTTGGGAGAGGCATCTGTTCATTGGAGCATGAGTGGATGCCAGAACTGTAGGTTATAAGGCAGTCACTTTTT CTCTCTACTCCCACCCACACCTGCCTCCCTCTTACCCCTGCTCCCCCACACTGCAGGAGGATTTGTCTCTAAGAGGTGCTGCCCCAAAGCTCCCCAAGCA TCAATACTCCTAGGGCTCAGGACAAGTGGCTCCCCTGGCCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGAT GCTGTCATTTTTTGAACCAAAAGACAAACAGGTTAAAAGGAAAAAAAGTAATCTGAATTTCCCAAGTGCCTACGCTGCATATTCCCCTTGTTAGATCCCA TTTTCATGTTACTTTGTAGCCTTGGCCAGAGGCTCAAAAAGGACACAACCAGTTTGGGGAAGGGGTGGCTAAGGAAGATGGTATAGGTGAAGGCGGCTGT GTGACCACTTTCCCCCACCCTTCCCACCCTCTAGACAACTCTCTCCCTTACCTGTTTTTGCTATGGCTGTAAAGGTATTTTTCCTCTGCCCCACTCCCTG CCATACCTTTATCCTGGGATCCTATTTTGGGCCTGGGGTGGGTATACCTGGGGCTGGTCTTAGGAGGGTGCTAGGCTGCAGACTGCCTTGTACTCCCTGG ACACCCTCAAATGGGGTTTTCTGTGTTATTTCATAAAATTCTTTGAAGTCCAATAAAGCATGTAGGAGATTTTAACCACT

STIM1.F1 (forward primer)
STIM1.R1 (reverse primer)
Single exon skipping

Frame 0

WKPLSWAWPVCVRLLDLGTASRLGTGLGGGADL GLGGPGSRRRPRPQDPAGRTPSPASAARRSSAGR GQVPPSRLSSLLFSSSSTSVPAETPAAPFRRGV VICGADSSPAATLPEVSGSGTSSGRRSPGGPTV GPEEPALLPHPNLEHLTFGCWRGQARGWLDSCGA ARASCLETVGCTPGLLALPLGSRGVHIRRMLTE T*SHGCMRPSCPVAPLGTPPAPGPEPQP*SQ*E GDRNQLGGQL*GVHCSRVLPN*QAPVSQ*G*ETQ LRGSP*HPQTDGR*CQW*CGCGRK**VPEGRPQ LP*PNSETQHLPW*G*AHQRGGPVEGMEVIRSI QLDRG*GGTVADHICGAASV*GDLPEAAAQWPCH AKAGCHQHHHDRDCAEDDRPESSAEAAAEGSGY SALWASSLDSP*SPQGLHAGGVYRYWCGRLLVC LYPEPLLQGAHEEDDEGLGGVTPS*AESA*PSGK AAQGPGGAPHSGGGEGPSGKEAAR*DQPC*AGS PAAEGAAGGY*E*AEPPKIC*GGVGAGSGGLEE SREGARISQLMVCSRGPSEVAAADT*GGGAILQH QEAKC*EAAAGGQGGG*EDKKEEKHTLWHLPRG PQLFPG*CRS*NSNS*ASTERGDSSIAGAPAPL ATDRDPLWLPDCQQPWHPLTGGCPQHRPQLDGQY TPQPCSLHHD*RRG*HG*GDCVSLVHAVP*PAE QCSAAPDGATAWPGISEGFDPFRFGVLPPHE*P PACGPQTSSDEPCCRRGSQCHDFQWQPPADRGGP PRVSGGETA*QPCPGQEGITGAEPWAGQGPQPD GAEPLSPTWWLSTFGFFPFSQPQLPRPRHTISS WGQPSPASQPKHTHSPPGWQEGCG*GG*WLYWRG NRLQPRPEEVSPQNL*EAS*EVGRMGWQ*RDSL SFPGCSVSPSLPSFKITGPKSGAWEGLVQGSGH CTYLPPHPWVLHYYLLTDHHGLPALPPSQPWAAA VTPSPLQCMS*LLFPQLPAPPLGFSFCLCCPSF EVWFLVSVSCFRAPPSHHSPTSPSSCREDRTSS F*HVCLRSVPPHSQWFCLLQTGARA*SLKFVLWY *CGSEGASS*SHSGLQGSMGE*NQFSENNPPET FKERPGLGMGWERHLFIGA*VDARTVGYKAVTF SLYSHPHLPPSYPCSPTLQEDLSLRGAAPKLPKH QYS*GSGQVAPLARRATAMIQGSYGALELLGKD AVIF*TKRQTG*KEKK*SEFPKCLRCIFPLLDP IFMLLCSLGQRLKKDTTSLGKGWLRKMV*VKAAV *PLSPTLPTL*TTLSLTCFCYGCKGIFPLPHSL PYLYPGILFWAWGGYTWGWS*EGARLQTALYSL DTLKWGFLCYFIKFFEVQ*SM*EILTT

Frame 1

GSRCPGPGRCASACWTWAPPAAWARDWAGALTS A*EAQDPGDARALRTLRVARPPQLLLLAALRQG EVRCPLLASLLFSSLPPPLLCPRRLRPPPSAGV* *SAELTAAPQPPCPKSPEAARAQAAAAPADPLL DLRSQPSSRTQTWST*PLAVGGGRLAGGWTAAE PRGHLAWRPSAALPGSWLCLWDPEVSTSDAC*LR PRVMDVCVRLALWLLWGLLLHQGQSLSHSHSEK ATGTSSGANSEESTAAEFCRIDKPLCHSEDEKL SFEAVRNIHKLMDDDANGDVDVEESDEFLREDLN YHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVY NWTVDEVVQWLITYVELPQYEETFRKLQLSGHA MPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDT VLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFA YIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQE RLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEA QRLKELREGTENERSRQKYAEEELEQVREALRK AEKELESHSSWYAPEALQKWLQLTHEVEVQYYN IKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVA HSSSLDDVDHKILTAKQALSEVTAALRERLHRW QQIEILCGFQIVNNPGIHSLVAALNIDPSWMGS TRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQS SVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDR QRVAPKPPQMSRAADEALNAMTSNGSHRLIEGV HPGSLVEKLPDSPALAKKALLALNHGLDKAHSLM ELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPV GDSRALQASRNTRIPHLAGKKAVAEEDNGSIGE ETDSSPGRKKFPLKIFKKPLKK*AGWGGSKGTAC PSLGVLSLLPSLPSR*LAPRVGHGKGWSRGLGT VHTCPLILGSFIIIY*LTTMACLPCLRPNHGLL LSLPLHFSACLSCCSLSSQLHLWGSASVSAVPVL RFGFLFLSLAFGLLPPTTPQLPLAVAGKIGRVA SDMCASDLFHPTHSGSVCSRLGLGPNL*SLFFG IDVGQKEPHPNLTQASRDPWGSETNSQRTTHQRL LKRGQAWEWVGRGICSLEHEWMPEL*VIRQSLF LSTPTHTCLPLTPAPPHCRRICL*EVLPQSSPS INTPRAQDKWLPWPGEPQP*YRALMEPWSCWARM LSFFEPKDKQVKRKKSNLNFPSAYAAYSPC*IP FSCYFVALARGSKRTQPVWGRGG*GRWYR*RRL CDHFPPPFPPSRQLSPLPVFAMAVKVFFLCPTPC HTFILGSYFGPGVGIPGAGLRRVLGCRLPCTPW TPSNGVFCVIS*NSLKSNKACRRF*P

Frame 2

EAAVLGLAGVRPPAGPGHRQPPGHGTGRGR*PR PRRPRIPETPAPSGPCGSHALPSFCCSPLFGRA RSGAPFSPLFSSLLFLLHFCARGDSGRPLPQGC SNLRS*QQPRSHPARSLRKRHELRPPQPRRTHCW T*GASPPPAPKLGALDLWLLEGAGSRVAGQLRS REGILPGDRRLHSRAPGFASGIPRCPHQTHVD* DLESWMYASVLPCGSSGDSSCTRARASAIVTVRR RQEPARGPTLRSPLQQSFAELTSPCVTVRMRNS ASRQSVTSTN*WTMMPMVMWMWKKVMSS*GKTS ITMTQQ*NTAPSMVRISSSAWRTCGRHGSHQKYT IGPWMRWYSG*SHMWSCLSMRRPSGSCSSVAMP CQGWLSPTPP*QGLC*R*QTGVIGRSCS*RLWI QCSLGLLS*LAIITSRTSCWWCLSLLVWAAAGLP ISRTVTPRST*RR**RTWRGYTELSRVCMTFRK GCTRPRRSTAQWRWRRSIWKRSCAMRSTLLSRK PSG*RSCGRVLRMSGAAKNMLRRSWSRFGRP*GK QRRS*NLTAHGMLQRPFRSGCS*HMRWRCNITT SRSKMLRSSCWWPRRGLRR*KRRETHSLAPSTW PTALPWMM*IIKF*QLSKH*AR*QQHCGSACTAG NRSRSSVASRLSTTLASTHWWLPST*TPAGWAV HAPTLLTSS*LTTWMTWMRRLCLPCPCSPLACR AVFGSA*RSHSMAWDLRGI*PIPIRSPPST*VTA SVWPPNLLR*AVLQTRLSMP*LPMAATG*SRGS TQGLWWRNCLTALPWPRRHYWR*TMGWTRPTA* WS*APQPHLVALHIWILPVLTAPAPQTQTHHLQL GTAEPCKPAETHAFPTWLARRLWLRRIMALLAR KQTPAQAGRSFPSKSLRSLLRSRQDGVAVKGQL VLPWVFCLSFPPFLQDNWPQEWGMGRAGPGVWAL YIPAPSSLGPSLLFIN*PPWPACPASVPTMGCC CHSLSTSVHVLVAVPSAPSSTSGVQLLSLLSQF *GLVSCFCLLLSGSSLPPLPNFP*QLQGR*DE*L LTCVPQICSTPLTVVLFAPDWG*GLIFEVCSLV LMWVRRSLILISLRPPGIHGGVKPILREQPTRD F*REARLGNGLGEASVHWSMSGCQNCRL*GSHFF SLLPPTPASLLPLLPHTAGGFVSKRCCPKAPQA SILLGLRTSGSPGQESHSHDTGLLWSPGVVGQG CCHFLNQKTNRLKGKKVI*ISQVPTLHIPLVRSH FHVTL*PWPEAQKGHNQFGEGVAKEDGIGEGGC VTTFPHPSHPLDNSLPYLFLLWL*RYFSSAPLP AIPLSWDPILGLGWVYLGLVLGGC*AADCLVLPG HPQMGFSVLFHKIL*SPIKHVGDFNH

Sequence STIM1.bAug05

CAGGAGTCCAAGAGGACTCCTAGCTCAATAGTTCCTCCTCATCTTCCCTAATCCACCCCTCTGCTACCACCCCAGTCATTTCTCAGCCATTAGGTTTTCC CTGGATCTTTTTGTCAAGCTTGCAGGGTATTCTACATTCATTGAACTTTCTAGATACCACTACATAAGAGGAATAATTGTGGCATTTGAAGTCACACAGA TTGGAATTCTAGCCTCAGTTCTTCCTACAAGTTGCAAGACTCTGTATCTGCCTGTCCCAGGCTGATAATGATACCAATATATCATTATCTGCTGGGAGGA CAAATGGGAAATACCTTGTAAACTGGTACTTGTTATGATTTTAGTATGAGATAGTTTCGAGGAGGGATTCAGATGCCTGAGAGCCTGGCTTCATAGCCTT GATTACTGGCAGCAAAATGATGGTCAGTGTGTGAATTAGGTAATGACTGTGGTTTCTATGGGCCTTGAGCTAGCTCAGAGCCAGACACACAGCAGAGCAG GGAGAATATATGCTGAGAGTTGGAGCTGTCATTTTCCTCTTTGATGCCATGACTCATGGCATGTTGGCTGGCACCCCCTTGCCTGGCCTCCTCCAGCTCC CTGCATTGCCCCCCCAGGCTGCACAAGGCCCAGGAGGAGCACCGCACAGTGGAGGTGGAGAAGGTCCATCTGGAAAAGAAGCTGCGCGATGAGATCAACC TTGCTAAGCAGGAAGCCCAGCGGCTGAAGGAGCTGCGGGAGGGTACTGAGAATGAGCGGAGCCGCCAAAAATATGCTGAGGAGGAGTTGGAGCAGGTTCG GGAGGCCTTGAGGAAAGCAGAGAAGGAGCTAGAATCTCACAGCTCATGGTATGCTCCAGAGGCCCTTCAGAAGTGGCTGCAGCTGACACATGAGGTGGAG GTGCAATATTACAACATCAAGAAGCAAAATGCTGAGAAGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAGATAAAAAAGAAGAGAAACACACTCTTTG GCACCTTCCACGTGGCCCACAGCTCTTCCCTGGATGATGTAGATCATAAAATTCTAACAGCTAAGCAAGCACTGAGCGAGGTGACAGCAGCATTGCGGGA GCGCCTGCACCGCTGGCAACAGATCGAGATCCTCTGTGGCTTCCAGATTGTCAACAACCCTGGCATCCACTCACTGGTGGCTGCCCTCAACATAGACCCC AGCTGGATGGGCAGTACACGCCCCAACCCTGCTCACTTCATCATGACTGACGACGTGGATGACATGGATGAGGAGATTGTGTCTCCCTTGTCCATGCAGT ATGCTGCCTGGCTGATGGGGCGTAGGTTCAGTGACCGCTCTCTCTGCTCTACATCCGCCGGCTCGGATGATCAGTCCCTCTGGAAATACCCCGCCCCTAG CCTGCAGAGCAGTGTTCGGCAGCGCCTGACGGAGCCACAGCATGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACATG AGTGACCGCCAGCGTGTGGCCCCCAAACCTCCTCAGATGAGCCGTGCTGCAGACGAGGCTCTCAATGCCATGACTTCCAATGGCAGCCACCGGCTGATCG AGGGGGTCCACCCAGGGTCTCTGGTGGAGAAACTGCCTGACAGCCCTGCCCTGGCCAAGAAGGCATTACTGGCGCTGAACCATGGGCTGGACAAGGCCCA CAGCCTGATGGAGCTGAGCCCCTCAGCCCCACCTGGTGGCTCTCCACATTTGGATTCTTCCCGTTCTCACAGCCCCAGCTCCCCAGACCCAGACACACCA TCTCCAGTTGGGGACAGCCGAGCCCTGCAAGCCAGCCGAAACACACGCATTCCCCACCTGGCTGGCAAGAAGGCTGTGGCTGAGGAGGATAATGGCTCTA TTGGCGAGGAAACAGACTCCAGCCCAGGCCGGAAGAAGTTTCCCCTCAAAATCTTTAAGAAGCCTCTTAAGAAGTAGGCAGGATGGGGTGGCAGTAAAGG GACAGCTTGTCCTTCCCTGGGTGTTCTGTCTCTCCTTCCCTCCCTTCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGAAGGGCTGGTCCAGGGGT CTGGGCACTGTACATACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTATTAACTGACCACCATGGCCTGCCTGCCCTGCCTCCGTCCCAACCATG GGCTGCTGCTGTCACTCCCTCTCCACTTCAGTGCATGTCTTAGTTGCTGTTCCCTCAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTCTCTGCTGT CCCAGTTTTGAGGTTTGGTTTCTTGTTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACCACTCCCCAACTTCCCCTAGCAGTTGCAGGGAAGATAGGA CGAGTAGCTTCTGACATGTGTGCCTCAGATCTGTTCCACCCCACTCACAGTGGTTCTGTTTGCTCCAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTGT TCTTTGGTATTGATGTGGGTCAGAAGGAGCCTCATCCTAATCTCACTCAGGCCTCCAGGGATCCATGGGGGAGTGAAACCAATTCTCAGAGAACAACCCA CCAGAGACTTTTAAAGAGAGGCCAGGCTTGGGAATGGGTTGGGAGAGGCATCTGTTCATTGGAGCATGAGTGGATGCCAGAACTGTAGGTTATAAGGCAG TCACTTTTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTACCCCTGCTCCCCCACACTGCAGGAGGATTTGTCTCTAAGAGGTGCTGCCCCAAAGCT CCCCAAGCATCAATACTCCTAGGGCTCAGGACAAGTGGCTCCCCTGGCCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTGGAGTTGTTG GGCAAGGATGCTGTCATTTTTTGAACCAAAAGACAAACAGGTTAAAAGGAAAAAAAGTAATCTGAATTTCCCAAGTGCCTACGCTGCATATTCCCCTTGT TAGATCCCATTTTCATGTTACTTTGTAGCCTTGGCCAGAGGCTCAAAAAGGACACAACCAGTTTGGGGAAGGGGTGGCTAAGGAAGATGGTATAGGTGAA GGCGGCTGTGTGACCACTTTCCCCCACCCTTCCCACCCTCTAGACAACTCTCTCCCTTACCTGTTTTTGCTATGGCTGTAAAGGTATTTTTCCTCTGCCC CACTCCCTGCCATACCTTTATCCTGGGATCCTATTTTGGGCCTGGGGTGGGTATACCTGGGGCTGGTCTTAGGAGGGTGCTAGGCTGCAGACTGCCTTGT ACTCCCTGGACACCCTCAAATGGGGTTTTCTGTGTTATTTCATAAAATTCTTTGAAGTCCAATAAAGCATGTAGGAGATTTTAACCACT

STIM1.F1 (forward primer)
STIM1.R1 (reverse primer)
Single exon skipping

Frame 0

QESKRTPSSIVPPHLP*STPLLPPQSFLSH*VF PGSFCQACRVFYIH*TF*IPLHKRNNCGI*SHTD WNSSLSSSYKLQDSVSACPRLIMIPIYHYLLGG QMGNTL*TGTCYDFSMR*FRGGIQMPESLAS*P *LLAAK*WSVCELGNDCGFYGP*ASSEPDTQQSR ENIC*ELELSFSSLMP*LMACWLAPPCLASSSS LHCPPRLHKAQEEHRTVEVEKVHLEKKLRDEIN LAKQEAQRLKELREGTENERSRQKYAEEELEQVR EALRKAEKELESHSSWYAPEALQKWLQLTHEVE VQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLF GTFHVAHSSSLDDVDHKILTAKQALSEVTAALRE RLHRWQQIEILCGFQIVNNPGIHSLVAALNIDP SWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQ YAAWLMGRRFSDRSLCSTSAGSDDQSLWKYPAPS LQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHM SDRQRVAPKPPQMSRAADEALNAMTSNGSHRLI EGVHPGSLVEKLPDSPALAKKALLALNHGLDKAH SLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTP SPVGDSRALQASRNTRIPHLAGKKAVAEEDNGS IGEETDSSPGRKKFPLKIFKKPLKK*AGWGGSKG TACPSLGVLSLLPSLPSR*LAPRVGHGKGWSRG LGTVHTCPLILGSFIIIY*LTTMACLPCLRPNH GLLLSLPLHFSACLSCCSLSSQLHLWGSASVSAV PVLRFGFLFLSLAFGLLPPTTPQLPLAVAGKIG RVASDMCASDLFHPTHSGSVCSRLGLGPNL*SL FFGIDVGQKEPHPNLTQASRDPWGSETNSQRTTH QRLLKRGQAWEWVGRGICSLEHEWMPEL*VIRQ SLFLSTPTHTCLPLTPAPPHCRRICL*EVLPQS SPSINTPRAQDKWLPWPGEPQP*YRALMEPWSCW ARMLSFFEPKDKQVKRKKSNLNFPSAYAAYSPC *IPFSCYFVALARGSKRTQPVWGRGG*GRWYR* RRLCDHFPPPFPPSRQLSPLPVFAMAVKVFFLCP TPCHTFILGSYFGPGVGIPGAGLRRVLGCRLPC TPWTPSNGVFCVIS*NSLKSNKACRRF*P

Frame 1

RSPRGLLAQ*FLLIFPNPPLCYHPSHFSAIRFS LDLFVKLAGYSTFIELSRYHYIRGIIVAFEVTQ IGILASVLPTSCKTLYLPVPG***YQYIIICWED KWEIPCKLVLVMILV*DSFEEGFRCLRAWLHSL DYWQQNDGQCVN*VMTVVSMGLELAQSQTHSRA GRIYAESWSCHFPL*CHDSWHVGWHPLAWPPPAP CIAPPGCTRPRRSTAQWRWRRSIWKRSCAMRST LLSRKPSG*RSCGRVLRMSGAAKNMLRRSWSRF GRP*GKQRRS*NLTAHGMLQRPFRSGCS*HMRWR CNITTSRSKMLRSSCWWPRRGLRR*KRRETHSL APSTWPTALPWMM*IIKF*QLSKH*AR*QQHCG SACTAGNRSRSSVASRLSTTLASTHWWLPST*TP AGWAVHAPTLLTSS*LTTWMTWMRRLCLPCPCS MLPG*WGVGSVTALSALHPPARMISPSGNTPPL ACRAVFGSA*RSHSMAWDLRGI*PIPIRSPPST* VTASVWPPNLLR*AVLQTRLSMP*LPMAATG*S RGSTQGLWWRNCLTALPWPRRHYWR*TMGWTRP TA*WS*APQPHLVALHIWILPVLTAPAPQTQTHH LQLGTAEPCKPAETHAFPTWLARRLWLRRIMAL LARKQTPAQAGRSFPSKSLRSLLRSRQDGVAVK GQLVLPWVFCLSFPPFLQDNWPQEWGMGRAGPGV WALYIPAPSSLGPSLLFIN*PPWPACPASVPTM GCCCHSLSTSVHVLVAVPSAPSSTSGVQLLSLL SQF*GLVSCFCLLLSGSSLPPLPNFP*QLQGR*D E*LLTCVPQICSTPLTVVLFAPDWG*GLIFEVC SLVLMWVRRSLILISLRPPGIHGGVKPILREQP TRDF*REARLGNGLGEASVHWSMSGCQNCRL*GS HFFSLLPPTPASLLPLLPHTAGGFVSKRCCPKA PQASILLGLRTSGSPGQESHSHDTGLLWSPGVV GQGCCHFLNQKTNRLKGKKVI*ISQVPTLHIPLV RSHFHVTL*PWPEAQKGHNQFGEGVAKEDGIGE GGCVTTFPHPSHPLDNSLPYLFLLWL*RYFSSA PLPAIPLSWDPILGLGWVYLGLVLGGC*AADCLV LPGHPQMGFSVLFHKIL*SPIKHVGDFNH

Frame 2

GVQEDS*LNSSSSSSLIHPSATTPVISQPLGFP WIFLSSLQGILHSLNFLDTTT*EE*LWHLKSHR LEF*PQFFLQVARLCICLSQADNDTNISLSAGR TNGKYLVNWYLL*F*YEIVSRRDSDA*EPGFIAL ITGSKMMVSV*IR**LWFLWALS*LRARHTAEQ GEYMLRVGAVIFLFDAMTHGMLAGTPLPGLLQL PALPPQAAQGPGGAPHSGGGEGPSGKEAAR*DQP C*AGSPAAEGAAGGY*E*AEPPKIC*GGVGAGS GGLEESREGARISQLMVCSRGPSEVAAADT*GG GAILQHQEAKC*EAAAGGQGGG*EDKKEEKHTLW HLPRGPQLFPG*CRS*NSNS*ASTERGDSSIAG APAPLATDRDPLWLPDCQQPWHPLTGGCPQHRP QLDGQYTPQPCSLHHD*RRG*HG*GDCVSLVHAV CCLADGA*VQ*PLSLLYIRRLG*SVPLEIPRP* PAEQCSAAPDGATAWPGISEGFDPFRFGVLPPH E*PPACGPQTSSDEPCCRRGSQCHDFQWQPPADR GGPPRVSGGETA*QPCPGQEGITGAEPWAGQGP QPDGAEPLSPTWWLSTFGFFPFSQPQLPRPRHT ISSWGQPSPASQPKHTHSPPGWQEGCG*GG*WLY WRGNRLQPRPEEVSPQNL*EAS*EVGRMGWQ*R DSLSFPGCSVSPSLPSFKITGPKSGAWEGLVQG SGHCTYLPPHPWVLHYYLLTDHHGLPALPPSQPW AAAVTPSPLQCMS*LLFPQLPAPPLGFSFCLCC PSFEVWFLVSVSCFRAPPSHHSPTSPSSCREDR TSSF*HVCLRSVPPHSQWFCLLQTGARA*SLKFV LWY*CGSEGASS*SHSGLQGSMGE*NQFSENNP PETFKERPGLGMGWERHLFIGA*VDARTVGYKA VTFSLYSHPHLPPSYPCSPTLQEDLSLRGAAPKL PKHQYS*GSGQVAPLARRATAMIQGSYGALELL GKDAVIF*TKRQTG*KEKK*SEFPKCLRCIFPL LDPIFMLLCSLGQRLKKDTTSLGKGWLRKMV*VK AAV*PLSPTLPTL*TTLSLTCFCYGCKGIFPLP HSLPYLYPGILFWAWGGYTWGWS*EGARLQTAL YSLDTLKWGFLCYFIKFFEVQ*SM*EILTT