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Sequences

Sequence GNB3.aAug05

GCGCCTGGAGCCTGTGATTTGTCAGGGAAATCTCAGCCACCTGCTCCCCTCATGCAAATGACCCAAGCCCCTGGCAGCTGCTCCTTAGGCCTCCAAGTGT
AAGCCCTGTCTTCCCTCTCTGAGCTTTGCTGAGACTTAGGGCCGCCTGCTTTCTCTCTGACCCCTGCTTTCACAGCCTCGAGAGGGCAAGGAGGGGGCCA
GACCCCAGCCTGGGAGCAGCGTGCGGGCCTTCCCTCCACTTGAAATCCGTTGCCCGCCCACAATAGGGGCAGACCTGTCCATCCTTCTCTGTGGGTCCCC
TGTACCTTTCTCCCCCAACAGGATCAGACCCAGAGGCAGCTGGTTGGGGTTTGTCGAGAAGAAGGATTATCCAGATCAGTCCTTTCTAATCTCAGCTCCT
GCCTGTACCCTCCCATACTCACCAAACCCTCTTCCCCACCACCCTGAGCTGAGGAGCACAGTTTGAGGCCCCCCCAACCCCCCGCCGGTCGGGGCCAGGC
CAGGCCAGGCCAGCTCCTCTGGCAGCAGAGCCTGGGCAGGTGACGGGCGGGCGCGGGCGTCGCAGCTGAGGGAGTAAGGAGGCTCCCAGGAACCGGAGCT
GGAAACCCGGCCGAGGTCCAGCCAGAGCCCAAGAGCCAGAGTGACCCCTCGACCTGTCAGCCATGGGGGAGATGGAGCAACTGCGTCAGGAAGCGGAGCA
GCTCAAGAAGCAGATTGCAGATGCCAGGAAAGCCTGTGCTGACGTTACTCTGGCAGAGCTGGTGTCTGGCCTAGAGGTGGTGGGACGAGTCCAGATGCGG
ACGCGGCGGACGTTAAGGGGACACCTGGCCAAGATTTACGCCATGCACTGGGCCACTGATTCTAAGCTGCTGGTAAGTGCCTCGCAAGATGGGAAGCTGA
TCGTGTGGGACAGCTACACCACCAACAAGGTGCACGCCATCCCACTGCGCTCCTCCTGGGTCATGACCTGTGCCTATGCCCCATCAGGGAACTTTGTGGC
ATGTGGGGGGCTGGACAACATGTGTTCCATCTACAACCTCAAATCCCGTGAGGGCAATGTCAAGGTCAGCCGGGAGCTTTCTGCTCACACAGGTTATCTC
TCCTGCTGCCGCTTCCTGGATGACAACAATATTGTGACCAGCTCGGGGGACACCACGTGTGCCTTGTGGGACATTGAGACTGGGCAGCAGAAGACTGTAT
TTGTGGGACACACGGGTGACTGCATGAGCCTGGCTGTGTCTCCTGACTTCAATCTCTTCATTTCGGGGGCCTGTGATGCCAGTGCCAAGCTCTGGGATGT
GCGAGAGGGGACCTGCCGTCAGACTTTCACTGGCCACGAGTCGGACATCAACGCCATCTGTTTCTTCCCCAATGGAGAGGCCATCTGCACGGGCTCGGAT
GACGCTTCCTGCCGCTTGTTTGACCTGCGGGCAGACCAGGAGCTGATCTGCTTCTCCCACGAGAGCATCATCTGCGGCATCACGTCCGTGGCCTTCTCCC
TCAGTGGCCGCCTACTATTCGCTGGCTACGACGACTTCAACTGCAATGTCTGGGACTCCATGAAGTCTGAGCGTGTGGGCATCCTCTCTGGCCACGATAA
CAGGGTGAGCTGCCTGGGAGTCACAGCTGACGGGATGGCTGTGGCCACAGGTTCCTGGGACAGCTTCCTCAAAATCTGGAACTGAGGAGGCTGGAGAAAG
GGAAGTGGAAGGCAGTGAACACACTCAGCAGCCCCCTGCCCGACCCCATCTCATTCAGGTGTTCTCTTCTATATTCCGGGTGCCATTCCCACTAAGCTTT
CTCCTTTGAGGGCAGTGGGGAGCATGGGACTGTGCCTTTGGGAGGCAGCATCAGGGACACAGGGGCAAAGAACTGCCCCATCTCCTCCCATGGCCTTCCC
TCCCCACAGTCCTCACAGCCTCTCCCTTAATGAGCAAGGACAACCTGCCCCTCCCCAGCCCTTTGCAGGCCCAGCAGACTTGAGTCTGAGGCCCCAGGCC
CTAGGATTCCTCCCCCAGAGCCACTACCTTTGTCCAGGCCTGGGTGGTATAGGGCGTTTGGCCCTGTGACTATGGCTCTGGCACCACTAGGGTCCTGGCC
CTCTTCTTATTCATGCTTTCTCCTTTTTCTACCTTTTTTTCTCTCCTAAGACACCTGCAATAAAGTGTAGCACCCTGGTAC
  • GNB3.F12 (forward primer)
  • GNB3.R2 (reverse primer)
  • Intron retention

Frame 0

APGACDLSGKSQPPAPLMQMTQAPGSCSLGLQV
*ALSSLSELC*DLGPPAFSLTPAFTASRGQGGGQ
TPAWEQRAGLPST*NPLPAHNRGRPVHPSLWVP
CTFLPQQDQTQRQLVGVCREEGLSRSVLSNLSS
CLYPPILTKPSSPPP*AEEHSLRPPQPPAGRGQA
RPGQLLWQQSLGR*RAGAGVAAEGVRRLPGTGA
GNPAEVQPEPKSQSDPSTCQPWGRWSNCVRKRS
SSRSRLQMPGKPVLTLLWQSWCLA*RWWDESRCG
RGGR*GDTWPRFTPCTGPLILSCW*VPRKMGS*
SCGTATPPTRCTPSHCAPPGS*PVPMPHQGTLW
HVGGWTTCVPSTTSNPVRAMSRSAGSFLLTQVIS
PAAASWMTTIL*PARGTPRVPCGTLRLGSRRLY
LWDTRVTA*AWLCLLTSISSFRGPVMPVPSSGM
CERGPAVRLSLATSRTSTPSVSSPMERPSARARM
TLPAACLTCGQTRS*SASPTRASSAASRPWPSP
SVAAYYSLATTTSTAMSGTP*SLSVWASSLATI
TG*AAWESQLTGWLWPQVPGTASSKSGTEEAGER
EVEGSEHTQQPPARPHLIQVFSSIFRVPFPLSF
LL*GQWGAWDCAFGRQHQGHRGKELPHLLPWPS
LPTVLTASPLMSKDNLPLPSPLQAQQT*V*GPRP
*DSSPRATTFVQAWVV*GVWPCDYGSGTTRVLA
LFLFMLSPFSTFFSLLRHLQ*SVAPWY

Frame 1

RLEPVICQGNLSHLLPSCK*PKPLAAAP*ASKC
KPCLPSLSFAET*GRLLSL*PLLSQPREGKEGA
RPQPGSSVRAFPPLEIRCPPTIGADLSILLCGSP
VPFSPNRIRPRGSWLGFVEKKDYPDQSFLISAP
ACTLPYSPNPLPHHPELRSTV*GPPNPPPVGAR
PGQASSSGSRAWAGDGRARASQLRE*GGSQEPEL
ETRPRSSQSPRARVTPRPVSHGGDGATASGSGA
AQEADCRCQESLC*RYSGRAGVWPRGGGTSPDA
DAADVKGTPGQDLRHALGH*F*AAGKCLARWEAD
RVGQLHHQQGARHPTALLLGHDLCLCPIRELCG
MWGAGQHVFHLQPQIP*GQCQGQPGAFCSHRLS
LLLPLPG*QQYCDQLGGHHVCLVGH*DWAAEDCI
CGTHG*LHEPGCVS*LQSLHFGGL*CQCQALGC
ARGDLPSDFHWPRVGHQRHLFLPQWRGHLHGLG
*RFLPLV*PAGRPGADLLLPREHHLRHHVRGLLP
QWPPTIRWLRRLQLQCLGLHEV*ACGHPLWPR*
QGELPGSHS*RDGCGHRFLGQLPQNLELRRLEK
GKWKAVNTLSSPLPDPISFRCSLLYSGCHSH*AF
SFEGSGEHGTVPLGGSIRDTGAKNCPISSHGLP
SPQSSQPLP**ARTTCPSPALCRPSRLESEAPG
PRIPPPEPLPLSRPGWYRAFGPVTMALAPLGSWP
SSYSCFLLFLPFFLS*DTCNKV*HPG

Frame 2

AWSL*FVREISATCSPHANDPSPWQLLLRPPSV
SPVFPL*ALLRLRAACFLSDPCFHSLERARRGP
DPSLGAACGPSLHLKSVARPQ*GQTCPSFSVGP
LYLSPPTGSDPEAAGWGLSRRRIIQISPF*SQLL
PVPSHTHQTLFPTTLS*GAQFEAPPTPRRSGPG
QARPAPLAAEPGQVTGGRGRRS*GSKEAPRNRS
WKPGRGPARAQEPE*PLDLSAMGEMEQLRQEAEQ
LKKQIADARKACADVTLAELVSGLEVVGRVQMR
TRRTLRGHLAKIYAMHWATDSKLLVSASQDGKL
IVWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVA
CGGLDNMCSIYNLKSREGNVKVSRELSAHTGYL
SCCRFLDDNNIVTSSGDTTCALWDIETGQQKTV
FVGHTGDCMSLAVSPDFNLFISGACDASAKLWDV
REGTCRQTFTGHESDINAICFFPNGEAICTGSD
DASCRLFDLRADQELICFSHESIICGITSVAFS
LSGRLLFAGYDDFNCNVWDSMKSERVGILSGHDN
RVSCLGVTADGMAVATGSWDSFLKIWN*GGWRK
GSGRQ*THSAAPCPTPSHSGVLFYIPGAIPTKL
SPLRAVGSMGLCLWEAASGTQGQRTAPSPPMAFP
PHSPHSLSLNEQGQPAPPQPFAGPADLSLRPQA
LGFLPQSHYLCPGLGGIGRLAL*LWLWHH*GPG
PLLIHAFSFFYLFFSPKTPAIKCSTLV

Sequence GNB3.bAug05

GCGCCTGGAGCCTGTGATTTGTCAGGGAAATCTCAGCCACCTGCTCCCCTCATGCAAATGACCCAAGCCCCTGGCAGCTGCTCCTTAGGCCTCCAAGTGT
AAGCCCTGTCTTCCCTCTCTGAGCTTTGCTGAGACTTAGGGCCGCCTGCTTTCTCTCTGACCCCTGCTTTCACAGCCTCGAGAGGGCAAGGAGGGGGCCA
GACCCCAGCCTGGGAGCAGCGTGCGGGCCTTCCCTCCACTTGAAATCCGTTGCCCGCCCACAATAGGGGCAGACCTGTCCATCCTTCTCTGTGGGTCCCC
TGTACCTTTCTCCCCCAACAGGATCAGACCCAGAGGCAGCTGGTTGGGGTTTGTCGAGAAGAAGGATTATCCAGATCAGTCCTTTCTAATCTCAGCTCCT
GCCTGTACCCTCCCATACTCACCAAACCCTCTTCCCCACCACCCTGAGCTGAGGAGCACAGTTTGAGGTACTGACAATGGGGCCATCTATCACCCTTATA
TCCCACCTCCTGGCCTGGTTGCTAAGTGGCCCTGACTGCCAAGATCATCACCATGGATGGGGGCCGGGACCAGGGGGGCCACTGGAGCTGTAGTTGTCTG
CTCCTTTGCACCCCTCCCAAACCCCAACCCTTAATCCTCACAGCTTTGCTCTAATCCTGTGGGGCCTGATGCTCTTATCTCTGCCTGCAGAGACTGACCA
GGGAAGCACCCCTTCACCCCCTCCTCTCTCAGGGCTTGTGGTGGGGGCTGCCCCTCTCTATGGCCCCACACTCCTGCCCCCAACCCTCCTCCCGGCTGGG
CCCAAGTCTCTCTGGAATCCGTACACCTCCCTCCCTCCCCCCACCCCTTCCCCCCACCCCTGCCAGCTGATTTCATTTGCCTGACGTTGTGGTTGGCTCT
ACCCTTCCCTGTCAGGCCCCCCCAACCCCCCGCCGGTCGGGGCCAGGCCAGGCCAGGCCAGCTCCTCTGGCAGCAGAGCCTGGGCAGGTGACGGGCGGGC
GCGGGCGTCGCAGCTGAGGGAGTAAGGAGGCTCCCAGGAACCGGAGCTGGAAACCCGGCCGAGGTCCAGCCAGAGCCCAAGAGCCAGAGTGACCCCTCGA
CCTGTCAGCCATGGGGGAGATGGAGCAACTGCGTCAGGAAGCGGAGCAGCTCAAGAAGCAGATTGCAGTAACTCCAGAGCCCTACCCCTGGGGCCCCAGA
AAACAGCTGGGGACATGAGGAGCGTGGCCCAGGGGGAGGGGGCTGGGTTTGCTCTTGAGTGACTAGAAGTGACCAGGGACTTTCTAAACTTGGTTCGCAT
ATTTGAGACCCCCAAGCCCCAGAGCCCCAAGACCAACCTGCCCTGAAGGCCCATGCACCTGCCACCCCCACCTCGCCCTTGCTCCCCTGATGTCTGCTTT
CCCTGCAG
GATGCCAGGAAAGCCTGTGCTGACGTTACTCTGGCAGAGCTGGTGTCTGGCCTAGAGGTGGTGGGACGAGTCCAGATGCGGACGCGGCGGAC
GTTAAGGGGACACCTGGCCAAGATTTACGCCATGCACTGGGCCACTGATTCTAAGCTGCTGGTAAGTGCCTCGCAAGATGGGAAGCTGATCGTGTGGGAC
AGCTACACCACCAACAAGGTGCACGCCATCCCACTGCGCTCCTCCTGGGTCATGACCTGTGCCTATGCCCCATCAGGGAACTTTGTGGCATGTGGGGGGC
TGGACAACATGTGTTCCATCTACAACCTCAAATCCCGTGAGGGCAATGTCAAGGTCAGCCGGGAGCTTTCTGCTCACACAGGTTATCTCTCCTGCTGCCG
CTTCCTGGATGACAACAATATTGTGACCAGCTCGGGGGACACCACGTGTGCCTTGTGGGACATTGAGACTGGGCAGCAGAAGACTGTATTTGTGGGACAC
ACGGGTGACTGCATGAGCCTGGCTGTGTCTCCTGACTTCAATCTCTTCATTTCGGGGGCCTGTGATGCCAGTGCCAAGCTCTGGGATGTGCGAGAGGGGA
CCTGCCGTCAGACTTTCACTGGCCACGAGTCGGACATCAACGCCATCTGTTTCTTCCCCAATGGAGAGGCCATCTGCACGGGCTCGGATGACGCTTCCTG
CCGCTTGTTTGACCTGCGGGCAGACCAGGAGCTGATCTGCTTCTCCCACGAGAGCATCATCTGCGGCATCACGTCCGTGGCCTTCTCCCTCAGTGGCCGC
CTACTATTCGCTGGCTACGACGACTTCAACTGCAATGTCTGGGACTCCATGAAGTCTGAGCGTGTGGGCATCCTCTCTGGCCACGATAACAGGGTGAGCT
GCCTGGGAGTCACAGCTGACGGGATGGCTGTGGCCACAGGTTCCTGGGACAGCTTCCTCAAAATCTGGAACTGAGGAGGCTGGAGAAAGGGAAGTGGAAG
GCAGTGAACACACTCAGCAGCCCCCTGCCCGACCCCATCTCATTCAGGTGTTCTCTTCTATATTCCGGGTGCCATTCCCACTAAGCTTTCTCCTTTGAGG
GCAGTGGGGAGCATGGGACTGTGCCTTTGGGAGGCAGCATCAGGGACACAGGGGCAAAGAACTGCCCCATCTCCTCCCATGGCCTTCCCTCCCCACAGTC
CTCACAGCCTCTCCCTTAATGAGCAAGGACAACCTGCCCCTCCCCAGCCCTTTGCAGGCCCAGCAGACTTGAGTCTGAGGCCCCAGGCCCTAGGATTCCT
CCCCCAGAGCCACTACCTTTGTCCAGGCCTGGGTGGTATAGGGCGTTTGGCCCTGTGACTATGGCTCTGGCACCACTAGGGTCCTGGCCCTCTTCTTATT
CATGCTTTCTCCTTTTTCTACCTTTTTTTCTCTCCTAAGACACCTGCAATAAAGTGTAGCACCCTGGTAC
  • GNB3.F12 (forward primer)
  • GNB3.R2 (reverse primer)
  • Intron retention

Frame 0

APGACDLSGKSQPPAPLMQMTQAPGSCSLGLQV
*ALSSLSELC*DLGPPAFSLTPAFTASRGQGGGQ
TPAWEQRAGLPST*NPLPAHNRGRPVHPSLWVP
CTFLPQQDQTQRQLVGVCREEGLSRSVLSNLSS
CLYPPILTKPSSPPP*AEEHSLRY*QWGHLSPLY
PTSWPGC*VALTAKIITMDGGRDQGGHWSCSCL
LLCTPPKPQPLILTALL*SCGA*CSYLCLQRLT
REAPLHPLLSQGLWWGLPLSMAPHSCPQPSSRLG
PSLSGIRTPPSLPPPLPPTPAS*FHLPDVVVGS
TLPCQAPPTPRRSGPGQARPAPLAAEPGQVTGG
RGRRS*GSKEAPRNRSWKPGRGPARAQEPE*PLD
LSAMGEMEQLRQEAEQLKKQIAVTPEPYPWGPR
KQLGT*GAWPRGRGLGLLLSD*K*PGTF*TWFA
YLRPPSPRAPRPTCPEGPCTCHPHLALAPLMSAF
PA
GCQESLC*RYSGRAGVWPRGGGTSPDADAAD
VKGTPGQDLRHALGH*F*AAGKCLARWEADRVG
QLHHQQGARHPTALLLGHDLCLCPIRELCGMWGA
GQHVFHLQPQIP*GQCQGQPGAFCSHRLSLLLP
LPG*QQYCDQLGGHHVCLVGH*DWAAEDCICGT
HG*LHEPGCVS*LQSLHFGGL*CQCQALGCARGD
LPSDFHWPRVGHQRHLFLPQWRGHLHGLG*RFL
PLV*PAGRPGADLLLPREHHLRHHVRGLLPQWP
PTIRWLRRLQLQCLGLHEV*ACGHPLWPR*QGEL
PGSHS*RDGCGHRFLGQLPQNLELRRLEKGKWK
AVNTLSSPLPDPISFRCSLLYSGCHSH*AFSFE
GSGEHGTVPLGGSIRDTGAKNCPISSHGLPSPQS
SQPLP**ARTTCPSPALCRPSRLESEAPGPRIP
PPEPLPLSRPGWYRAFGPVTMALAPLGSWPSSY
SCFLLFLPFFLS*DTCNKV*HPG

Frame 1

RLEPVICQGNLSHLLPSCK*PKPLAAAP*ASKC
KPCLPSLSFAET*GRLLSL*PLLSQPREGKEGA
RPQPGSSVRAFPPLEIRCPPTIGADLSILLCGSP
VPFSPNRIRPRGSWLGFVEKKDYPDQSFLISAP
ACTLPYSPNPLPHHPELRSTV*GTDNGAIYHPY
IPPPGLVAKWP*LPRSSPWMGAGTRGATGAVVVC
SFAPLPNPNP*SSQLCSNPVGPDALISACRD*P
GKHPFTPSSLRACGGGCPSLWPHTPAPNPPPGW
AQVSLESVHLPPSPHPFPPPLPADFICLTLWLAL
PFPVRPPQPPAGRGQARPGQLLWQQSLGR*RAG
AGVAAEGVRRLPGTGAGNPAEVQPEPKSQSDPS
TCQPWGRWSNCVRKRSSSRSRLQ*LQSPTPGAPE
NSWGHEERGPGGGGWVCS*VTRSDQGLSKLGSH
I*DPQAPEPQDQPALKAHAPATPTSPLLP*CLL
SLQ
DARKACADVTLAELVSGLEVVGRVQMRTRRT
LRGHLAKIYAMHWATDSKLLVSASQDGKLIVWD
SYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGG
LDNMCSIYNLKSREGNVKVSRELSAHTGYLSCCR
FLDDNNIVTSSGDTTCALWDIETGQQKTVFVGH
TGDCMSLAVSPDFNLFISGACDASAKLWDVREG
TCRQTFTGHESDINAICFFPNGEAICTGSDDASC
RLFDLRADQELICFSHESIICGITSVAFSLSGR
LLFAGYDDFNCNVWDSMKSERVGILSGHDNRVS
CLGVTADGMAVATGSWDSFLKIWN*GGWRKGSGR
Q*THSAAPCPTPSHSGVLFYIPGAIPTKLSPLR
AVGSMGLCLWEAASGTQGQRTAPSPPMAFPPHS
PHSLSLNEQGQPAPPQPFAGPADLSLRPQALGFL
PQSHYLCPGLGGIGRLAL*LWLWHH*GPGPLLI
HAFSFFYLFFSPKTPAIKCSTLV

Frame 2

AWSL*FVREISATCSPHANDPSPWQLLLRPPSV
SPVFPL*ALLRLRAACFLSDPCFHSLERARRGP
DPSLGAACGPSLHLKSVARPQ*GQTCPSFSVGP
LYLSPPTGSDPEAAGWGLSRRRIIQISPF*SQLL
PVPSHTHQTLFPTTLS*GAQFEVLTMGPSITLI
SHLLAWLLSGPDCQDHHHGWGPGPGGPLEL*LS
APLHPSQTPTLNPHSFALILWGLMLLSLPAETDQ
GSTPSPPPLSGLVVGAAPLYGPTLLPPTLLPAG
PKSLWNPYTSLPPPTPSPHPCQLISFA*RCGWL
YPSLSGPPNPPPVGARPGQASSSGSRAWAGDGRA
RASQLRE*GGSQEPELETRPRSSQSPRARVTPR
PVSHGGDGATASGSGAAQEADCSNSRALPLGPQ
KTAGDMRSVAQGEGAGFALE*LEVTRDFLNLVRI
FETPKPQSPKTNLP*RPMHLPPPPRPCSPDVCF
PCR
MPGKPVLTLLWQSWCLA*RWWDESRCGRGG
R*GDTWPRFTPCTGPLILSCW*VPRKMGS*SCGT
ATPPTRCTPSHCAPPGS*PVPMPHQGTLWHVGG
WTTCVPSTTSNPVRAMSRSAGSFLLTQVISPAA
ASWMTTIL*PARGTPRVPCGTLRLGSRRLYLWDT
RVTA*AWLCLLTSISSFRGPVMPVPSSGMCERG
PAVRLSLATSRTSTPSVSSPMERPSARARMTLP
AACLTCGQTRS*SASPTRASSAASRPWPSPSVAA
YYSLATTTSTAMSGTP*SLSVWASSLATITG*A
AWESQLTGWLWPQVPGTASSKSGTEEAGEREVE
GSEHTQQPPARPHLIQVFSSIFRVPFPLSFLL*G
QWGAWDCAFGRQHQGHRGKELPHLLPWPSLPTV
LTASPLMSKDNLPLPSPLQAQQT*V*GPRP*DS
SPRATTFVQAWVV*GVWPCDYGSGTTRVLALFLF
MLSPFSTFFSLLRHLQ*SVAPWY

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